IMP GPI Biosynthesis Report IMP-Bioinformatics
GPI Biosynthesis Main Page  
GPI Site Motif   GPI Site Prediction   Home Page B.E.  

GPI Anchor Biosynthesis Report: CAC67556.1 (PIG-K family, Trypanosoma brucei)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (319 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 5

Distribution of 50 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

emb|CAC67556.1| (AJ308106) Gpi8 transamidase [Trypanosoma brucei] 492 e-138
emb|CAB55340.1| (AJ242865) GPI:protein transamidase [Leishmania ... 431 e-120
ref|NP_563825.1| (NM_100749) putative GPI-anchor transamidase (U... 418 e-116
pir||T12043 probable legumain (EC 3.4.22.34) precursor - kidney ... 410 e-113
emb|CAC18099.1| (AJ251453) putative legumain [Zea mays] 409 e-113
emb|CAB64544.1| (AJ131718) legumain-like protease [Zea mays] 408 e-113
dbj|BAA76744.1| (D89971) asparaginyl endopeptidase (VmPE-1) [Vig... 408 e-112
gb|AAK15049.1|AF238384_1 (AF238384) asparaginyl endopeptidase [V... 407 e-112
emb|CAB64545.1| (AJ131719) legumain-like protease [Zea mays] 406 e-112
sp|P49043|VPE_CITSI VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) >... 406 e-112
sp|P49045|VPE_SOYBN VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) >... 405 e-112
sp|P49046|LEGU_CANEN Legumain precursor (Asparaginyl endopeptida... 405 e-112
emb|CAB16318.1| (Z99174) cysteine proteinase precursor [Vicia na... 404 e-112
gb|AAF69014.1|AF260827_1 (AF260827) cysteine protease [Ipomoea b... 404 e-111
dbj|BAA76745.1| (D89972) asparaginyl endopeptidase (VmPE-1A) [Vi... 403 e-111
dbj|BAA84650.1| (AB025310) asparaginyl endopeptidase [Oryza sati... 402 e-111
sp|P49042|VPE_RICCO Vacuolar processing enzyme precursor (VPE) >... 401 e-111
gb|AAD04883.1| (AF082347) C13 endopeptidase NP1 precursor [Zea m... 401 e-111
pir||T12044 probable legumain (EC 3.4.22.34) precursor - kidney ... 401 e-110
pir||S60050 vacuolar processing enzyme (EC 3.4.22.-) isozyme bet... 400 e-110
emb|CAC18100.1| (AJ251454) putative legumain [Zea mays] 400 e-110
emb|CAC43295.1| (AJ309173) putative vacuolar processing enzyme [... 399 e-110
ref|NP_195020.1| (NM_119448) gamma-VPE (vacuolar processing enzy... 399 e-110
ref|NP_176458.1| (NM_104948) beta-VPE [Arabidopsis thaliana] >gi... 399 e-110
sp|Q39119|VPEG_ARATH VACUOLAR PROCESSING ENZYME, GAMMA-ISOZYME P... 399 e-110
sp|P49044|VPE_VICSA VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) (... 398 e-110
ref|NP_180165.1| (NM_128154) putative vacuolar processing enzyme... 397 e-110
ref|XP_039644.1| (XM_039644) phosphatidylinositol glycan, class ... 397 e-109
emb|CAA68871.1| (Y07596) gpi8 [Homo sapiens] 397 e-109
gb|AAF89646.1|AF169019_1 (AF169019) seed maturation protein PM40... 397 e-109
pir||T10944 cysteine proteinase (EC 3.4.22.-) precursor - spring... 394 e-108
gb|AAF89679.1| (AF169973) asparaginyl endopeptidase [Sesamum ind... 393 e-108
pir||S60049 vacuolar processing enzyme (EC 3.4.22.-) isozyme alp... 392 e-108
gb|AAF99765.1|AC003981_15 (AC003981) F22O13.24 [Arabidopsis thal... 389 e-107
pir||T00731 hypothetical protein F22O13.26 - Arabidopsis thaliana 386 e-106
dbj|BAB29018.1| (AK013853) data source:SPTR, source key:O14822, ... 381 e-105
gb|AAF73260.1|AF154349_1 (AF154349) legumain [Rattus norvegicus] 375 e-103
ref|NP_071562.1| (NM_022226) protease, cysteine, 1 (legumain) [R... 374 e-102
ref|NP_035305.1| (NM_011175) protease, cysteine, 1; preproleguma... 373 e-102
dbj|BAA09530.1| (D55696) cysteine protease [Homo sapiens] 370 e-101
ref|XP_007399.4| (XM_007399) legumain [Homo sapiens] 370 e-101
gb|AAH03061.1|AAH03061 (BC003061) protease, cysteine, 1 (legumai... 368 e-101
emb|CAB51545.1| (AJ243876) vacuolar processing enzyme [Lycopersi... 368 e-101
ref|NP_005597.1| (NM_005606) legumain; protease, cysteine, 1 (le... 368 e-101
pir||T40853 probable cysteine proteinase (EC 3.4.22.-) [similari... 367 e-100
dbj|BAB69947.1| (AB060129) legumain [Bos taurus] 365 e-100
gb|AAF45703.1| (AE003422) EG:133E12.3 gene product [Drosophila m... 363 3e-99
ref|NP_188656.1| (NM_112912) vacuolar processing enzyme/asparagi... 358 9e-98
ref|NP_010618.1| (NC_001136) Protein involved in the attachment ... 357 1e-97
emb|CAB42651.1| (AJ238881) putative preprolegumain [Nicotiana ta... 353 2e-96
Alignments
>emb|CAC67556.1| (AJ308106) Gpi8 transamidase [Trypanosoma brucei]
          Length = 319

 Score =  492 bits (1254), Expect = e-138
 Identities = 319/319 (100%), Positives = 319/319 (100%)

Query: 1   MLPMLLWLVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG 60
           MLPMLLWLVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG
Sbjct: 1   MLPMLLWLVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG 60

Query: 61  MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120
           MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV
Sbjct: 61  MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120

Query: 121 LQGRYDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQR 180
           LQGRYDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQR
Sbjct: 121 LQGRYDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQR 180

Query: 181 RYRKLVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFL 240
           RYRKLVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFL
Sbjct: 181 RYRKLVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFL 240

Query: 241 EVLKDSKCRPENGEVTLLQKSFYDFNYGPERLSLPQPLSEPAHFDAVNRPNAIREWKMDE 300
           EVLKDSKCRPENGEVTLLQKSFYDFNYGPERLSLPQPLSEPAHFDAVNRPNAIREWKMDE
Sbjct: 241 EVLKDSKCRPENGEVTLLQKSFYDFNYGPERLSLPQPLSEPAHFDAVNRPNAIREWKMDE 300

Query: 301 FFCEQDRDKIPVELRYDLF 319
           FFCEQDRDKIPVELRYDLF
Sbjct: 301 FFCEQDRDKIPVELRYDLF 319
>emb|CAB55340.1| (AJ242865) GPI:protein transamidase [Leishmania mexicana]
          Length = 349

 Score =  431 bits (1098), Expect = e-120
 Identities = 170/316 (53%), Positives = 220/316 (68%), Gaps = 12/316 (3%)

Query: 15  APAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDSY 74
           A A  G  G  ++N WAVI+SSSRY FN RHT NAL MYHL R+HG+DDDHIL+FLSDS+
Sbjct: 33  ASAPLGATGKGQSNNWAVIVSSSRYLFNYRHTANALTMYHLLRQHGIDDDHILLFLSDSF 92

Query: 75  ACDPRKPNPATIYGAPAQAEQPN------LYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
           ACDPR   PA I+  P  A   +      LYGC+ +VDYA  DV VRRFL VLQGRYDEN
Sbjct: 93  ACDPRNVYPAEIFSQPPGAHDADGRASMNLYGCSAQVDYAGSDVDVRRFLSVLQGRYDEN 152

Query: 129 TPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFL 188
           TPP+RRL +D  SNIIIY AGH A+ +FKFQD+EF+SS+DI++TL MM +QRRY ++VFL
Sbjct: 153 TPPTRRLLSDNTSNIIIYVAGHGAKSYFKFQDTEFLSSSDISETLTMMHQQRRYGRVVFL 212

Query: 189 VDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVLKDSKC 248
            DTC A++LC  ++APNVVCLA+S+A  +SYS   D   G  +++ W  E   +L  + C
Sbjct: 213 ADTCHAIALCEHVEAPNVVCLAASDAESESYSCQYDEQLGTHMVSFWMNEMYLLLNGTSC 272

Query: 249 ------RPENGEVTLLQKSFYDFNYGPERLSLPQPLSEPAHFDAVNRPNAIREWKMDEFF 302
                 R  +  V++L +S+Y+FNY P R+   +  S+PAH DAVN P A+REW + +F 
Sbjct: 273 SNPLTRRIGDDAVSVLHQSWYNFNYHPYRVEASRNRSKPAHRDAVNDPTALREWIVADFV 332

Query: 303 CEQDRDKIPVELRYDL 318
           C Q    +PV++RYDL
Sbjct: 333 CGQVSAAVPVDVRYDL 348
>ref|NP_563825.1| (NM_100749) putative GPI-anchor transamidase (U32517) [Arabidopsis
           thaliana]
          Length = 388

 Score =  418 bits (1066), Expect = e-116
 Identities = 98/281 (34%), Positives = 163/281 (57%), Gaps = 12/281 (4%)

Query: 5   LLWLVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDD 64
           +L LV  L  +  +        TN WAV++ +SR++FN RH  N L++Y   ++ G+ D+
Sbjct: 3   ILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFWFNYRHMANTLSLYRTVKRLGIPDE 62

Query: 65  HILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGR 124
            I++ L+D  AC+ R   PA ++    +  + NLYG N+ VDY  Y+V V  FL VL GR
Sbjct: 63  RIILMLADDMACNARNEYPAQVFN--NENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGR 120

Query: 125 YDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRK 184
           ++   P S+RL +DE S+I++Y  GH  ++F KFQD+E + S D+AD +  M E+RR+++
Sbjct: 121 HENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKE 180

Query: 185 LVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVLK 244
           L+ +VDTC+A +L  ++++P V+ + SS    +SYSHHLD   G +V+ R+T+  L   +
Sbjct: 181 LMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFE 240

Query: 245 ------DSKCR---PENGEVTLLQKSFYDFN-YGPERLSLP 275
                 ++             L+  ++Y  + Y P  + +P
Sbjct: 241 RLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVP 281
>pir||T12043 probable legumain (EC 3.4.22.34) precursor - kidney bean
 emb|CAB17078.1| (Z99956) asparagine-specific endopeptidase precursor [Phaseolus
           vulgaris]
          Length = 484

 Score =  410 bits (1045), Expect = e-113
 Identities = 58/264 (21%), Positives = 104/264 (38%), Gaps = 17/264 (6%)

Query: 8   LVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHIL 67
           LV +    P+  G         WA++ + S  ++N RH  +    Y L RK G+ D++I+
Sbjct: 28  LVGDFLRLPSDSGNGDNVHGTRWAILFAGSSGYWNYRHQADICHAYQLLRKGGLKDENII 87

Query: 68  VFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDE 127
           VF+ D  A +   P    I  +P       +Y   +  DY   DV    F   L G   +
Sbjct: 88  VFMYDDIAFNSENPRRGVIINSPN---GDEVYK-GVPKDYTGEDVTAHNFYAALLGDKSK 143

Query: 128 NTPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLV 186
            T  S + +++  N +I I+ + H            ++ ++D+ + L        Y+ LV
Sbjct: 144 LTGGSGKVVNSGPNDHIFIFYSDHGGPGVLGSPAGPYIYASDLNEVLKKKHASGTYKNLV 203

Query: 187 FLVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFE 238
           F ++ C + S+   +     NV    +S A   S+  +        PP   T +      
Sbjct: 204 FYLEACESGSIFEGLLPEDINVYATTASNADESSWGTYCPGEDPSPPPEYSTCLGD--LY 261

Query: 239 FLEVLKDSKCRPENGEVTLLQKSF 262
            +  ++DS     N     L + +
Sbjct: 262 SVAWMEDS--DRHNLRTETLHQQY 283
>emb|CAC18099.1| (AJ251453) putative legumain [Zea mays]
          Length = 486

 Score =  409 bits (1041), Expect = e-113
 Identities = 60/258 (23%), Positives = 105/258 (40%), Gaps = 17/258 (6%)

Query: 14  LAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDS 73
            A AA+     +    WAV+++ S  ++N RH  +    Y + +K G+ D++I+VF+ D 
Sbjct: 35  RAAAADETDDGDVGTRWAVLIAGSSGYYNYRHQADICHAYQIMKKGGLKDENIIVFMYDD 94

Query: 74  YACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS- 132
            A  P  P P  I   P   +  ++Y   +  DY   DV V  F  VL G        S 
Sbjct: 95  IAHSPENPRPGVIINHP---QGGDVYA-GVPKDYTGRDVNVDNFFAVLLGNKTALRGGSG 150

Query: 133 RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTC 192
           + +D+  N +I ++ + H            ++   D+ D L        Y+ LVF ++ C
Sbjct: 151 KVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEAC 210

Query: 193 RALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFLEVLK 244
            + S+   +     NV    +S A   S+  +        PP   T +       +  ++
Sbjct: 211 ESGSIFEGLLPNDINVYATTASNADESSWGTYCPGEFPSPPPEYDTCLGD--LYSVAWME 268

Query: 245 DSKCRPENGEVTLLQKSF 262
           DS     N     L++ +
Sbjct: 269 DS--DFHNLRTESLKQQY 284
>emb|CAB64544.1| (AJ131718) legumain-like protease [Zea mays]
          Length = 486

 Score =  408 bits (1038), Expect = e-113
 Identities = 59/258 (22%), Positives = 104/258 (39%), Gaps = 17/258 (6%)

Query: 14  LAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDS 73
            A AA+          WAV+++ S  ++N RH  +    Y + +K G+ D++I+VF+ D 
Sbjct: 35  RAAAADETDDGAVGTRWAVLIAGSSGYYNYRHQADICHAYQIMKKGGLKDENIIVFMYDD 94

Query: 74  YACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS- 132
            A  P  P P  I   P   +  ++Y   +  DY   DV V  F  VL G        S 
Sbjct: 95  IAHSPENPRPGVIINHP---QGGDVYA-GVPKDYTGRDVNVDNFFAVLLGNKTALRGGSG 150

Query: 133 RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTC 192
           + +D+  + +I ++ + H            ++   D+ D L        Y+ LVF ++ C
Sbjct: 151 KVVDSGPDDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEAC 210

Query: 193 RALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFLEVLK 244
            + S+   +     NV    +S A   S+  +        PP   T +       +  ++
Sbjct: 211 ESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLGD--LYSVAWME 268

Query: 245 DSKCRPENGEVTLLQKSF 262
           DS     N     L++ +
Sbjct: 269 DS--DFHNLRTESLKQQY 284
>dbj|BAA76744.1| (D89971) asparaginyl endopeptidase (VmPE-1) [Vigna mungo]
          Length = 483

 Score =  408 bits (1038), Expect = e-112
 Identities = 59/261 (22%), Positives = 106/261 (40%), Gaps = 20/261 (7%)

Query: 11  NLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFL 70
             F APA +          WAV+++ S  ++N RH ++    Y L  K G+ +++I+VF+
Sbjct: 33  RFFQAPATDENDE---GTRWAVLIAGSNGYWNYRHQSDVCHAYQLLTKGGLKEENIVVFM 89

Query: 71  SDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTP 130
            D  A +   P P  I  +P      ++Y   +  DY   DV V  F   + G     T 
Sbjct: 90  YDDIAFNEENPRPGVIINSP---HGNDVYK-GVPKDYVGEDVTVNNFFAAILGNKSALTG 145

Query: 131 PS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLV 189
            S + +++  N +I IY + H          S +M ++D+ + L        Y+ L F +
Sbjct: 146 GSGKVVNSGPNDHIFIYYSDHGGPGVLGMPTSPYMYASDLIEVLKKKHASGTYKSLAFYL 205

Query: 190 DTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFLE 241
           + C + S+   +     N+    ++ A   S+  +        PP   T +       + 
Sbjct: 206 EGCESGSIFGGLLPEGLNIYATTAANAEESSWGTYCPGDNPSPPPEYETCLGD--LYSVA 263

Query: 242 VLKDSKCRPENGEVTLLQKSF 262
            ++DS     N     L + F
Sbjct: 264 WMEDS--DIHNLRTETLHQQF 282
>gb|AAK15049.1|AF238384_1 (AF238384) asparaginyl endopeptidase [Vigna radiata]
          Length = 483

 Score =  407 bits (1037), Expect = e-112
 Identities = 56/264 (21%), Positives = 103/264 (38%), Gaps = 17/264 (6%)

Query: 8   LVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHIL 67
           LV +    P+  G     K   WA++ + S  ++N RH  +    Y + RK G+ +++I+
Sbjct: 28  LVGDFLRLPSDSGNDDNVKGTRWAILFAGSNGYWNYRHQADICHAYQILRKGGLKEENII 87

Query: 68  VFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDE 127
           VF+ D  A +   P P  I   P      ++Y   +  DY   D     F   L G    
Sbjct: 88  VFMYDDIAFNWDNPRPGVIINKPD---GDDVY-EGVPKDYTGEDATAHNFYSALLGDKSA 143

Query: 128 NTPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLV 186
            T  S + +++  +  I I+ + H            ++ ++D+ + L        Y+ LV
Sbjct: 144 LTGGSGKVVNSGPDDRIFIFYSDHGGPGVLGTPAGPYIYASDLVEVLKKKHASGTYKNLV 203

Query: 187 FLVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFE 238
           F ++ C A S+   +     N+    +S A   S+  +        PP   T +      
Sbjct: 204 FYLEACEAGSIFEGLLPEDINIYATTASNAEESSWGTYCPGEYPSPPPEYSTCLGD--LY 261

Query: 239 FLEVLKDSKCRPENGEVTLLQKSF 262
            +  ++DS     N     L + +
Sbjct: 262 SVAWMEDS--DRHNLRTESLHQQY 283
>emb|CAB64545.1| (AJ131719) legumain-like protease [Zea mays]
          Length = 481

 Score =  406 bits (1034), Expect = e-112
 Identities = 58/259 (22%), Positives = 102/259 (38%), Gaps = 17/259 (6%)

Query: 14  LAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDS 73
             P+            WAV+++ S  ++N RH  +    Y + +K G+ D++I+VF+ D 
Sbjct: 30  RLPSDRAAADDAVGTRWAVLIAGSNGYYNYRHQADICHAYQIMKKGGLKDENIVVFMYDD 89

Query: 74  YACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS- 132
            A  P  P P  I   P   +  ++Y   +  DY   DV V  F  VL G        S 
Sbjct: 90  IAHSPENPRPGVIINHP---QGGDVYA-GVPKDYTGRDVNVDNFFAVLLGNKTALRGGSG 145

Query: 133 RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTC 192
           + +D+  N +I ++ + H            ++   D+ D L        Y+ LVF ++ C
Sbjct: 146 KVVDSGPNDHISVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEAC 205

Query: 193 RALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFLEVLK 244
            + S+   +     NV    +S A   S+  +        PP   T +       +  ++
Sbjct: 206 ESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLGD--LYSVAWME 263

Query: 245 DSKCRPENGEVTLLQKSFY 263
           DS     N     L++ + 
Sbjct: 264 DS--DFHNLRTESLKQQYN 280
>sp|P49043|VPE_CITSI VACUOLAR PROCESSING ENZYME PRECURSOR (VPE)
 pir||S51117 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - sweet
           orange
 emb|CAA87720.1| (Z47793) cystein proteinase (by similarity) [Citrus sinensis]
 prf||2208463A vascular processing protease [Citrus sinensis]
          Length = 494

 Score =  406 bits (1033), Expect = e-112
 Identities = 61/255 (23%), Positives = 104/255 (39%), Gaps = 17/255 (6%)

Query: 17  AAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDSYAC 76
           A       +    WAV+L+ S  F+N RH  +    Y L RK G+ D++I+VF+ D  A 
Sbjct: 48  AKVNDDDDSVGTRWAVLLAGSNGFWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAF 107

Query: 77  DPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS-RRL 135
           +   P P  I   P      ++Y   +  DY   DV V +F  V+ G     T  S + +
Sbjct: 108 NEENPRPGVIINHP---HGDDVYK-GVPKDYTGEDVTVEKFFAVVLGNKTALTGGSGKVV 163

Query: 136 DTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTCRAL 195
           D+  N +I I+ + H          S ++ + ++ D L        Y+ LVF ++ C + 
Sbjct: 164 DSGPNDHIFIFYSDHGGPGVLGMPTSRYIYADELIDVLKKKHASGNYKSLVFYLEACESG 223

Query: 196 SLCLEIK--APNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFLEVLKDSK 247
           S+   +     N+    +S A   S+  +        PP   T +       +  ++DS 
Sbjct: 224 SIFEGLLLEGLNIYATTASNAEESSWGTYCPGEIPGPPPEYSTCLGD--LYSIAWMEDS- 280

Query: 248 CRPENGEVTLLQKSF 262
               N     L + +
Sbjct: 281 -DIHNLRTETLHQQY 294
>sp|P49045|VPE_SOYBN VACUOLAR PROCESSING ENZYME PRECURSOR (VPE)
 pir||T07132 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - soybean
 dbj|BAA06030.1| (D28876) cysteine proteinase [Glycine max]
          Length = 495

 Score =  405 bits (1032), Expect = e-112
 Identities = 54/263 (20%), Positives = 101/263 (37%), Gaps = 19/263 (7%)

Query: 9   VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
           V  L   P             WAV+++ S  + N RH  +    Y L  K G+ +++I+V
Sbjct: 42  VIKLPTEPVDADSDE--VGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGLKEENIVV 99

Query: 69  FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
           F+ D  A +   P    I   P   E  +LY   +  DY   +V       V+ G   + 
Sbjct: 100 FMYDDIATNELNPRHGVIINHP---EGEDLYA-GVPKDYTGDNVTTENLFAVILGDKSKL 155

Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
              S + +++     I IY + H         +  ++ + D  D L        Y+++V 
Sbjct: 156 KGGSGKVINSKPEDRIFIYYSDHGGPGILGMPNMPYLYAMDFIDVLKKKHASGSYKEMVI 215

Query: 188 LVDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
            V+ C + S+   I     N+    +S A  +S+  +        PP   T +       
Sbjct: 216 YVEACESGSVFEGIMPKDLNIYVTTASNAQENSWGTYCPGMDPSPPPEYITCLGD--LYS 273

Query: 240 LEVLKDSKCRPENGEVTLLQKSF 262
           +  ++DS+    N +   +++ +
Sbjct: 274 VAWMEDSEAH--NLKRESVKQQY 294
>sp|P49046|LEGU_CANEN Legumain precursor (Asparaginyl endopeptidase)
 pir||JX0344 legumain (EC 3.4.22.34) precursor - jack bean
 dbj|BAA06596.1| (D31787) asparaginyl endopeptidase (Legumain) [Canavalia
           ensiformis]
          Length = 475

 Score =  405 bits (1031), Expect = e-112
 Identities = 54/263 (20%), Positives = 105/263 (39%), Gaps = 22/263 (8%)

Query: 9   VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
           V  L   P  +          WAV+++ S  + N RH  +    Y L  K G+ +++I+V
Sbjct: 25  VIQLPTEPVDDE-----VGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVV 79

Query: 69  FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
           F+ D  A +   P P  I   P   + P++Y   +  DY   DV       V+ G   + 
Sbjct: 80  FMYDDIAYNAMNPRPGVIINHP---QGPDVYA-GVPKDYTGEDVTPENLYAVILGDKSKV 135

Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
              S + ++++    I I+ + H         ++ F+ + D  D L        Y+++V 
Sbjct: 136 KGGSGKVINSNPEDRIFIFYSDHGGPGVLGMPNAPFVYAMDFIDVLKKKHASGGYKEMVI 195

Query: 188 LVDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
            ++ C + S+   I     N+    +S A  +S+  +        P    T +       
Sbjct: 196 YIEACESGSIFEGIMPKDLNIYVTTASNAQENSFGTYCPGMNPPPPEEYVTCLGD--LYS 253

Query: 240 LEVLKDSKCRPENGEVTLLQKSF 262
           +  ++DS+    N +   +Q+ +
Sbjct: 254 VSWMEDSETH--NLKRETVQQQY 274
>emb|CAB16318.1| (Z99174) cysteine proteinase precursor [Vicia narbonensis]
          Length = 488

 Score =  404 bits (1030), Expect = e-112
 Identities = 58/263 (22%), Positives = 104/263 (39%), Gaps = 18/263 (6%)

Query: 9   VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
           V  L   P  +          WAV+++ S  + N RH  +    Y L  K G+ +++I+V
Sbjct: 34  VIRLPGEPV-DADVEDEMGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVV 92

Query: 69  FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
           F+ D  A +   P P  I   P   + PN+Y   +  DY    V    F  V+ G   + 
Sbjct: 93  FMYDDIAYNEMNPRPGVIINHP---QGPNVY-DGVPKDYNGDFVTAENFYAVILGDKSKV 148

Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
              S + +++     I IY + H         +  ++ + D  D L        Y+K+V 
Sbjct: 149 RGGSGKVINSKAEDRIFIYCSDHGGPGVLGMPNMPYVYAMDFIDVLKKKHASGGYKKMVI 208

Query: 188 LVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
            V+ C + S+   I     NV    +S A  +S+  +        PP   T +       
Sbjct: 209 YVEACESGSIFEGIMPKDINVYVTTASNAQENSWGTYCPGVDPAPPPEYITCLGD--LYS 266

Query: 240 LEVLKDSKCRPENGEVTLLQKSF 262
           +  ++DS+    N +   +++ +
Sbjct: 267 VAWMEDSETH--NLKRETVKQQY 287
>gb|AAF69014.1|AF260827_1 (AF260827) cysteine protease [Ipomoea batatas]
          Length = 492

 Score =  404 bits (1028), Expect = e-111
 Identities = 58/267 (21%), Positives = 104/267 (38%), Gaps = 21/267 (7%)

Query: 10  ANLFLAPAAEG----FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDH 65
           A  F  PA E         +    WAV+++ S  ++N RH  +    Y + +  G+ D++
Sbjct: 32  ARRFFRPAEEENREADGDDSVGTRWAVLIAGSNGYWNYRHQADICHAYQILKAGGLKDEN 91

Query: 66  ILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRY 125
           I+VF+ D  A +   P    I  +P      ++Y   +  DY   DV     L V+ G  
Sbjct: 92  IVVFMYDDIAYNEENPRKGIIINSP---HGEDVY-HGVPKDYTGDDVTANNLLAVILGDK 147

Query: 126 DENTPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRK 184
                 S + +D+  N +I IY + H          S ++ + ++   L        Y+ 
Sbjct: 148 SAVKGGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPYLYADELNAALKKKHAAGAYKS 207

Query: 185 LVFLVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWT 236
           LVF ++ C + S+   I     N+    +S A   S+  +        PP   T +    
Sbjct: 208 LVFYLEACESGSIFEGILPKDINIYATTASNAIESSWGTYCPGEYPSPPPEYETCLGD-- 265

Query: 237 FEFLEVLKDSKCRPENGEVTLLQKSFY 263
              +  ++DS     N     L++ + 
Sbjct: 266 LYSIAWMEDS--DIHNLRTESLKQQYN 290
>dbj|BAA76745.1| (D89972) asparaginyl endopeptidase (VmPE-1A) [Vigna mungo]
          Length = 482

 Score =  403 bits (1027), Expect = e-111
 Identities = 54/263 (20%), Positives = 101/263 (37%), Gaps = 17/263 (6%)

Query: 9   VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
           V +    P+  G     +   WA++ + S  ++N RH  +    Y + RK G+ +++I+V
Sbjct: 28  VGDFLRLPSDSGNDDNVQGTRWAILFAGSNGYWNYRHQADICHAYQILRKGGLKEENIIV 87

Query: 69  FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
           F+ D  A +   P P  I   P      ++Y   +  DY   D     F   L G     
Sbjct: 88  FMYDDIAFNWDNPRPGVIINKPD---GDDVY-EGVPKDYTGEDATAHNFYSALLGDKSAL 143

Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
           T  S + + +  +  I I+ + H            ++ ++D+ + L        Y+ LVF
Sbjct: 144 TGGSGKVVSSGPDDRIFIFYSDHGGPGVLGTPAGPYIYASDLVEVLKKKHASGTYKNLVF 203

Query: 188 LVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
            ++ C A S+   +     N+    +S A   S+  +        PP   T +       
Sbjct: 204 YLEACEAGSIFEGLLPEDINIYATTASNAEESSWGTYCPGEYPSPPPEYSTCLGD--LYS 261

Query: 240 LEVLKDSKCRPENGEVTLLQKSF 262
           +  ++DS     N     L + +
Sbjct: 262 VAWMEDS--DRHNLRTESLHQQY 282
>dbj|BAA84650.1| (AB025310) asparaginyl endopeptidase [Oryza sativa]
 dbj|BAB85400.1| (AP003924) asparaginyl endopeptidase [Oryza sativa (japonica
           cultivar-group)]
          Length = 501

 Score =  402 bits (1023), Expect = e-111
 Identities = 58/256 (22%), Positives = 105/256 (40%), Gaps = 17/256 (6%)

Query: 17  AAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDSYAC 76
           AA+      +   WAV+++ S  ++N RH  +    Y + ++ G+ D++I+VF+ D  A 
Sbjct: 53  AADDAAEAAEGTRWAVLIAGSNGYYNYRHQADVCHAYQIMKRGGLKDENIIVFMYDDIAH 112

Query: 77  DPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS-RRL 135
           +P  P P  I   P   +  ++Y   +  DY   +V V+    VL G        S + L
Sbjct: 113 NPENPRPGVIINHP---QGGDVYA-GVPKDYTGKEVNVKNLFAVLLGNKTAVKGGSGKVL 168

Query: 136 DTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTCRAL 195
           D+  N +I I+ + H            ++   D+ D L        Y+ LVF ++ C + 
Sbjct: 169 DSGPNDHIFIFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEACESG 228

Query: 196 SLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFLEVLKDSK 247
           S+   +     NV    +S A   S+  +        PP   T +       +  ++DS 
Sbjct: 229 SIFEGLLPNGINVYATTASNADESSWGTYCPGEYPSPPPEYDTCLGD--LYSVAWMEDS- 285

Query: 248 CRPENGEVTLLQKSFY 263
               N     L++ + 
Sbjct: 286 -DVHNLRTESLKQQYN 300
>sp|P49042|VPE_RICCO Vacuolar processing enzyme precursor (VPE)
 pir||JQ2387 vacuolar processing enzyme (EC 3.4.22.-) precursor - castor bean
 dbj|BAA04225.1| (D17401) precursor of vacuolar processing enzyme [Ricinus communis]
          Length = 497

 Score =  401 bits (1022), Expect = e-111
 Identities = 56/264 (21%), Positives = 101/264 (38%), Gaps = 22/264 (8%)

Query: 21  FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDSYACDPRK 80
                    WAV+++ S  F N RH  +    Y L RK G+ +++I+VF+ D  A +   
Sbjct: 54  DDDDQLGTRWAVLVAGSMGFGNYRHQADVCHAYQLLRKGGLKEENIIVFMYDDIAKNELN 113

Query: 81  PNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS-RRLDTDE 139
           P P  I   P   +  ++Y   +  DY    V  +    VL G        S + +D+  
Sbjct: 114 PRPGVIINHP---QGEDVYA-GVPKDYTGEHVTAKNLYAVLLGDKSAVQGGSGKVVDSKP 169

Query: 140 NSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTCRALSLCL 199
           N  I +Y + H         +  ++ + D  + L        Y+K+V  V+ C + S+  
Sbjct: 170 NDRIFLYYSDHGGPGVLGMPNLPYLYAMDFIEVLKKKHAAGGYKKMVIYVEACESGSIFE 229

Query: 200 EIKAPNV--VCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFLEVLKDSKCRPE 251
            I   +V      +S A   S+  +        PP   T +       +  ++DS+    
Sbjct: 230 GIMPKDVDIYVTTASNAQESSWGTYCPGMEPSPPPEFTTCLGD--LYSVAWMEDSESH-- 285

Query: 252 NGEVTLLQKSFYD-----FNYGPE 270
           N +   +++ +        NY   
Sbjct: 286 NLKKETVKQQYSSVKARTSNYNTY 309
>gb|AAD04883.1| (AF082347) C13 endopeptidase NP1 precursor [Zea mays]
          Length = 485

 Score =  401 bits (1021), Expect = e-111
 Identities = 60/264 (22%), Positives = 104/264 (38%), Gaps = 18/264 (6%)

Query: 10  ANLFLA-PAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
             L     AA+          WAV+++ S  ++N RH  +    Y + +K G+ D++I+V
Sbjct: 29  IRLPSERAAADETDDDAVGTRWAVLIAGSNGYYNYRHQADICHAYQIMKKGGLKDENIVV 88

Query: 69  FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
           F+ D  A  P  P P  I   P   +  ++Y   +  DY   DV V  F  VL G     
Sbjct: 89  FMYDDIAHSPENPRPGVIINHP---QGGDVYA-GVPKDYTGRDVNVDNFFAVLLGNKTAL 144

Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
              S + +D+  N +I ++ + H            ++   D+ D L        Y+ LVF
Sbjct: 145 RGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVF 204

Query: 188 LVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
            ++ C + S+   +     NV    +S A   S+  +        PP   T +       
Sbjct: 205 YLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLGD--LYS 262

Query: 240 LEVLKDSKCRPENGEVTLLQKSFY 263
           +  ++DS     N     L++ + 
Sbjct: 263 VAWMEDS--DFHNLRTESLKQQYN 284
>pir||T12044 probable legumain (EC 3.4.22.34) precursor - kidney bean
 emb|CAB17079.1| (Z99957) legumain-like proteinase precursor [Phaseolus vulgaris]
          Length = 493

 Score =  401 bits (1020), Expect = e-110
 Identities = 54/263 (20%), Positives = 101/263 (37%), Gaps = 19/263 (7%)

Query: 9   VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
           V  L                 WAV+++ S  + N RH  +    Y L  K G+ +++I+V
Sbjct: 40  VIKLPTQEVDAESDE--VGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVV 97

Query: 69  FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
           F+ D  A     P P  I   P   + P++Y   +  DY    V    F  VL G   + 
Sbjct: 98  FMYDDIATHELNPRPGVIINNP---QGPDVYA-GVPKDYTGESVTSHNFFAVLLGDKSKV 153

Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
              S + +++     I +Y + H         +  ++ + D  D L        Y+++V 
Sbjct: 154 KGGSGKVINSKPEDRIFVYYSDHGGPGVLGMPNMPYLYAMDFIDVLKKKHASGGYKEMVI 213

Query: 188 LVDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHLD------PPSGFTVITRWTFEF 239
            V+ C + S+   I     N+    +S A  +S+  +        PP   T +       
Sbjct: 214 YVEACESGSIFEGIMPKDLNIYVTTASNAQENSWGTYCPGMYPPPPPEYITCLGD--LYS 271

Query: 240 LEVLKDSKCRPENGEVTLLQKSF 262
           +  ++DS+    N +   +++ +
Sbjct: 272 VAWMEDSESH--NLKKESVEQQY 292
>pir||S60050 vacuolar processing enzyme (EC 3.4.22.-) isozyme beta precursor -
           Arabidopsis thaliana
 dbj|BAA09615.1| (D61394) beta-VPE [Arabidopsis thaliana]
          Length = 484

 Score =  400 bits (1018), Expect = e-110
 Identities = 56/274 (20%), Positives = 103/274 (37%), Gaps = 21/274 (7%)

Query: 10  ANLFLAPAAEGF-HGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
             +    A             WAV+++ S  + N RH  +    Y + RK G+ +++I+V
Sbjct: 31  ILMPTEEANPADQDEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVV 90

Query: 69  FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
            + D  A  P  P P T+   P      ++Y   +  DY    V    F  VL G     
Sbjct: 91  LMYDDIANHPLNPRPGTLINHPD---GDDVYA-GVPKDYTGSSVTAANFYAVLLGDQKAV 146

Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
              S + + +  N +I +Y A H         ++  + + D  +TL        Y+++V 
Sbjct: 147 KGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVI 206

Query: 188 LVDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
            V+ C + S+   I     N+    +S A   SY  +        P    T +       
Sbjct: 207 YVEACESGSIFEGIMPKDLNIYVTTASNAQESSYGTYCPGMNPSPPSEYITCLGD--LYS 264

Query: 240 LEVLKDSKCRPENGEVTLLQKSFY---DFNYGPE 270
           +  ++DS+    N +   +++ ++     NY   
Sbjct: 265 VAWMEDSETH--NLKKETIKQQYHTMRTSNYNTY 296
>emb|CAC18100.1| (AJ251454) putative legumain [Zea mays]
          Length = 485

 Score =  400 bits (1018), Expect = e-110
 Identities = 59/264 (22%), Positives = 104/264 (39%), Gaps = 18/264 (6%)

Query: 10  ANLFLA-PAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
             L     AA+          WAV+++ S  ++N RH  +    Y + +K G+ D++I+V
Sbjct: 29  IRLPSERAAADETDDDAVGTRWAVLIAGSNGYYNYRHQADICHAYQIMKKGGLKDENIVV 88

Query: 69  FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
           F+ D  A  P  P P  I   P   +  ++Y   +  DY   +V V  F  VL G     
Sbjct: 89  FMYDDIAHSPENPRPGVIINHP---QGGDVYA-GVPKDYTGREVNVDNFFAVLLGNKTAL 144

Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
              S + +D+  N +I ++ + H            ++   D+ D L        Y+ LVF
Sbjct: 145 RGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVF 204

Query: 188 LVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
            ++ C + S+   +     NV    +S A   S+  +        PP   T +       
Sbjct: 205 YLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLGD--LYS 262

Query: 240 LEVLKDSKCRPENGEVTLLQKSFY 263
           +  ++DS     N     L++ + 
Sbjct: 263 VAWMEDS--DFHNLRTESLKQQYN 284
>emb|CAC43295.1| (AJ309173) putative vacuolar processing enzyme [Beta vulgaris]
          Length = 486

 Score =  399 bits (1016), Expect = e-110
 Identities = 58/269 (21%), Positives = 110/269 (40%), Gaps = 21/269 (7%)

Query: 7   WLVANLFLAPAAE----GFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMD 62
            +V NL   P+           +    WAV+++ S  ++N RH  +    Y + +K G+ 
Sbjct: 25  LMVDNLIRWPSDHPSIFESDDDSVGTRWAVLIAGSSGYWNYRHQADVCHAYQVLKKGGLK 84

Query: 63  DDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQ 122
           D++I+VF+ D  A D   P P  +  +P      ++Y   +  DY   DV V  F   + 
Sbjct: 85  DENIIVFMYDDIAYDEENPRPGVLINSP---YGHDVYA-GVPKDYTGEDVTVNNFFAAIL 140

Query: 123 GRYDENTPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRR 181
           G  D  T  S + +++  N +I I+ + H            ++ + ++ +TL        
Sbjct: 141 GNKDAITGGSGKVVNSGPNDHIFIFYSDHGGAGVLGMPTYPYLYADELIETLKEKHASGT 200

Query: 182 YRKLVFLVDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHLD------PPSGFTVIT 233
           Y+ LV  ++ C + S+   I     N+    +S A   S+  +        PP   T + 
Sbjct: 201 YKSLVVYIEACESGSIFEGILPEGLNIYATTASNAVESSWGTYCPGQDPNVPPEYDTCLG 260

Query: 234 RWTFEFLEVLKDSKCRPENGEVTLLQKSF 262
                 +  ++DS+    N     L++ +
Sbjct: 261 D--LYSVSWIEDSE--RHNLHTESLKQQY 285
>ref|NP_195020.1| (NM_119448) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
           thaliana]
 pir||T05302 vacuolar processing enzyme (EC 3.4.22.-) isozyme gamma precursor -
           Arabidopsis thaliana
 emb|CAA21203.1| (AL031804) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
           thaliana]
 emb|CAB80011.1| (AL161582) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
           thaliana]
 gb|AAK43975.1|AF370160_1 (AF370160) putative gamma-VPE protein [Arabidopsis thaliana]
 gb|AAL11612.1|AF424619_1 (AF424619) AT4g32940/F26P21_60 [Arabidopsis thaliana]
 gb|AAL15210.1| (AY059104) putative gamma-VPE protein [Arabidopsis thaliana]
          Length = 494

 Score =  399 bits (1016), Expect = e-110
 Identities = 63/262 (24%), Positives = 109/262 (41%), Gaps = 18/262 (6%)

Query: 10  ANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVF 69
           A+ F  PA E     N    WAV+++ S  ++N RH  +    Y L RK G+ +++I+VF
Sbjct: 41  ASRFFRPA-ENDDDSNSGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVF 99

Query: 70  LSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENT 129
           + D  A +   P P TI  +P      ++Y   +  DY   DV V     V+ G      
Sbjct: 100 MYDDIANNYENPRPGTIINSP---HGKDVYQ-GVPKDYTGDDVNVDNLFAVILGDKTAVK 155

Query: 130 PPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFL 188
             S + +D+  N +I I+ + H          S ++ + D+ D L        Y+ LVF 
Sbjct: 156 GGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFY 215

Query: 189 VDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFL 240
           ++ C + S+   +     N+    +S A   S+  +        PP   T +       +
Sbjct: 216 LEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEEPSPPPEYETCLGD--LYSV 273

Query: 241 EVLKDSKCRPENGEVTLLQKSF 262
             ++DS     N +   L + +
Sbjct: 274 AWMEDSG--MHNLQTETLHQQY 293
>ref|NP_176458.1| (NM_104948) beta-VPE [Arabidopsis thaliana]
 gb|AAK56243.1|AF367254_1 (AF367254) At1g62710/F23N19_8 [Arabidopsis thaliana]
 gb|AAL15381.1| (AY059156) At1g62710/F23N19_8 [Arabidopsis thaliana]
          Length = 486

 Score =  399 bits (1015), Expect = e-110
 Identities = 56/276 (20%), Positives = 103/276 (37%), Gaps = 23/276 (8%)

Query: 10  ANLFLAPAAEGF-HGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
             +    A             WAV+++ S  + N RH  +    Y + RK G+ +++I+V
Sbjct: 31  ILMPTEEANPADQDEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVV 90

Query: 69  FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
            + D  A  P  P P T+   P      ++Y   +  DY    V    F  VL G     
Sbjct: 91  LMYDDIANHPLNPRPGTLINHPD---GDDVYA-GVPKDYTGSSVTAANFYAVLLGDQKAV 146

Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
              S + + +  N +I +Y A H         ++  + + D  +TL        Y+++V 
Sbjct: 147 KGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVI 206

Query: 188 LVDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
            V+ C + S+   I     N+    +S A   SY  +        P    T +       
Sbjct: 207 YVEACESGSIFEGIMPKDLNIYVTTASNAQESSYGTYCPGMNPSPPSEYITCLGD--LYS 264

Query: 240 LEVLKDSKCRPENGEVTLLQKSFY-----DFNYGPE 270
           +  ++DS+    N +   +++ ++       NY   
Sbjct: 265 VAWMEDSETH--NLKKETIKQQYHTVKMRTSNYNTY 298
>sp|Q39119|VPEG_ARATH VACUOLAR PROCESSING ENZYME, GAMMA-ISOZYME PRECURSOR (GAMMA-VPE)
 dbj|BAA18924.1| (D61395) gamma-VPE [Arabidopsis thaliana]
          Length = 490

 Score =  399 bits (1015), Expect = e-110
 Identities = 63/262 (24%), Positives = 109/262 (41%), Gaps = 18/262 (6%)

Query: 10  ANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVF 69
           A+ F  PA E     N    WAV+++ S  ++N RH  +    Y L RK G+ +++I+VF
Sbjct: 37  ASRFFRPA-ENDDDSNSGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVF 95

Query: 70  LSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENT 129
           + D  A +   P P TI  +P      ++Y   +  DY   DV V     V+ G      
Sbjct: 96  MYDDIANNYENPRPGTIINSP---HGKDVYQ-GVPKDYTGDDVNVDNLFAVILGDKTAVK 151

Query: 130 PPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFL 188
             S + +D+  N +I I+ + H          S ++ + D+ D L        Y+ LVF 
Sbjct: 152 GGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFY 211

Query: 189 VDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFL 240
           ++ C + S+   +     N+    +S A   S+  +        PP   T +       +
Sbjct: 212 LEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEEPSPPPEYETCLGD--LYSV 269

Query: 241 EVLKDSKCRPENGEVTLLQKSF 262
             ++DS     N +   L + +
Sbjct: 270 AWMEDSG--MHNLQTETLHQQY 289
>sp|P49044|VPE_VICSA VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) (PROTEINASE B)
 pir||S49175 legumain (EC 3.4.22.34) precursor [similarity] - spring vetch
 pir||S68984 cysteine proteinase precursor - spring vetch
 emb|CAA84383.1| (Z34899) cysteine proteinase [Vicia sativa]
          Length = 493

 Score =  398 bits (1013), Expect = e-110
 Identities = 56/273 (20%), Positives = 104/273 (37%), Gaps = 24/273 (8%)

Query: 11  NLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFL 70
             F  P  +          WA++L+ S  ++N RH ++    Y L RK G  +++I+VF+
Sbjct: 41  RFFREPKNDDDFE---GTRWAILLAGSNGYWNYRHQSDVCHAYQLLRKGGSKEENIIVFM 97

Query: 71  SDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTP 130
            D  A +   P P  I   P      ++Y   +  DY   +V    F   L G     T 
Sbjct: 98  YDDIASNEENPRPGVIINKPD---GDDVYA-GVPKDYTGAEVHADNFYAALLGNKSALTG 153

Query: 131 PS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLV 189
            S + +D+  N +I +Y   H            ++ ++D+ + L        Y+ LVF +
Sbjct: 154 GSGKVVDSGPNDHIFVYYTDHGGPGVLGMPVGPYLYASDLNEVLKKKHASGTYKSLVFYL 213

Query: 190 DTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHLDPP------SGFTVITRWTFEFLE 241
           + C + S+   +     N+    +S A   S+ ++             T +       + 
Sbjct: 214 EACESGSIFEGLLPDDLNIYATTASNAEESSWGYYCPGDKPPPPPEYSTCLGD--LYSIA 271

Query: 242 VLKDSKCRPENGEVTLLQKSF----YDFNYGPE 270
            ++DS+    N +   LQ+ +          P 
Sbjct: 272 WMEDSE--VHNLQTESLQQQYKLVKNRTISEPY 302
>ref|NP_180165.1| (NM_128154) putative vacuolar processing enzyme [Arabidopsis
           thaliana]
 sp|P49047|VPEA_ARATH VACUOLAR PROCESSING ENZYME, ALPHA-ISOZYME PRECURSOR (ALPHA-VPE)
 pir||T02629 vacuolar processing enzyme (EC 3.4.22.-) isozyme alpha precursor -
           Arabidopsis thaliana
 gb|AAC31241.1| (AC004747) vacuolar processing enzyme, alpha-isozyme precursor
           (alpha-VPE) [Arabidopsis thaliana]
 dbj|BAA09614.2| (D61393) alpha-VPE [Arabidopsis thaliana]
 gb|AAL24163.1| (AY058055) At2g25940/F17H15.3 [Arabidopsis thaliana]
          Length = 478

 Score =  397 bits (1012), Expect = e-110
 Identities = 58/262 (22%), Positives = 107/262 (40%), Gaps = 21/262 (8%)

Query: 10  ANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVF 69
           A+ F  P          +  WAV+++ S  ++N RH  +    Y L +K G+ +++I+VF
Sbjct: 29  ASKFFRPTENDDD----STKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVF 84

Query: 70  LSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENT 129
           + D  A +   P P  I  +P      ++Y   +  DY   +V V   L V+ G      
Sbjct: 85  MYDDIAKNEENPRPGVIINSPN---GEDVY-NGVPKDYTGDEVNVDNLLAVILGNKTALK 140

Query: 130 PPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFL 188
             S + +D+  N +I IY + H          S  + + D+ D L   +    Y+ LVF 
Sbjct: 141 GGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFY 200

Query: 189 VDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFL 240
           ++ C + S+   +     N+    +S A   S+  +        P    T +       +
Sbjct: 201 LEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGD--LYSV 258

Query: 241 EVLKDSKCRPENGEVTLLQKSF 262
             ++DS+    N +   L + +
Sbjct: 259 AWIEDSEKH--NLQTETLHEQY 278
>ref|XP_039644.1| (XM_039644) phosphatidylinositol glycan, class K [Homo sapiens]
 gb|AAB81597.1| (AF022913) GPI transamidase [Homo sapiens]
 gb|AAH20737.1|AAH20737 (BC020737) Unknown (protein for MGC:22559) [Homo sapiens]
          Length = 395

 Score =  397 bits (1011), Expect = e-109
 Identities = 102/267 (38%), Positives = 158/267 (58%), Gaps = 11/267 (4%)

Query: 9   VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
           VA   +   AE F     TN WAV++ +SR++FN RH  N L++Y   ++ G+ D HI++
Sbjct: 25  VAASHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVL 84

Query: 69  FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
            L+D  AC+PR P PAT++    +  + N+YG ++ VDY SY+V V  FL VL GR   +
Sbjct: 85  MLADDMACNPRNPKPATVFSH--KNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIPPS 142

Query: 129 TPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFL 188
           TP S+RL +D+ SNI+IY  GH    F KFQDSE +++ ++AD    MW++RRY +L+F+
Sbjct: 143 TPRSKRLLSDDRSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFI 202

Query: 189 VDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVLKD--- 245
           +DTC+  S+     +PN++ LASS+   DS SH  DP  G  ++ R+TF  LE L++   
Sbjct: 203 IDTCQGASMYERFYSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINP 262

Query: 246 -SKCRPENG-----EVTLLQKSFYDFN 266
            S+    +      +   +    +  +
Sbjct: 263 ASQTNMNDLFQVCPKSLCVSTPGHRTD 289
>emb|CAA68871.1| (Y07596) gpi8 [Homo sapiens]
          Length = 396

 Score =  397 bits (1010), Expect = e-109
 Identities = 102/267 (38%), Positives = 158/267 (58%), Gaps = 11/267 (4%)

Query: 9   VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
           VA   +   AE F     TN WAV++ +SR++FN RH  N L++Y   ++ G+ D HI++
Sbjct: 26  VAASHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVL 85

Query: 69  FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
            L+D  AC+PR P PAT++    +  + N+YG ++ VDY SY+V V  FL VL GR   +
Sbjct: 86  MLADDMACNPRNPKPATVFSH--KNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIPPS 143

Query: 129 TPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFL 188
           TP S+RL +D+ SNI+IY  GH    F KFQDSE +++ ++AD    MW++RRY +L+F+
Sbjct: 144 TPRSKRLLSDDRSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFI 203

Query: 189 VDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVLKD--- 245
           +DTC+  S+     +PN++ LASS+   DS SH  DP  G  ++ R+TF  LE L++   
Sbjct: 204 IDTCQGASMYERFYSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINP 263

Query: 246 -SKCRPENG-----EVTLLQKSFYDFN 266
            S+    +      +   +    +  +
Sbjct: 264 ASQTNMNDLFQVCPKSLCVSTPGHRTD 290
>gb|AAF89646.1|AF169019_1 (AF169019) seed maturation protein PM40 [Glycine max]
          Length = 496

 Score =  397 bits (1010), Expect = e-109
 Identities = 53/264 (20%), Positives = 104/264 (39%), Gaps = 19/264 (7%)

Query: 9   VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
           V  L   P             WAV+++ S  + N RH  +    Y L  K G+ +++I+V
Sbjct: 41  VIKLPAEPVDADSDHE-VGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGLKEENIVV 99

Query: 69  FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
           F+ D  A D   P P  I   P   E  ++Y   +  DY   +V  +    V+ G  ++ 
Sbjct: 100 FMYDDIATDELNPRPGVIINHP---EGQDVYA-GVPKDYTGENVTAQNLFAVILGDKNKV 155

Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
              S + +++     I IY + H         +  ++ + D  + L        Y+K+V 
Sbjct: 156 KGGSGKVINSKPEDRIFIYYSDHGGPGVLGMPNMPYLYAMDFIEVLKKKHASGGYKKMVI 215

Query: 188 LVDTCRALS-LCLEIKAPN--VVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFE 238
            V+ C + + +   I   +  +    +S A  +S+  +        PP   T +      
Sbjct: 216 YVEACESGNHVLKGIMPKDLQIYVTTASNAQENSWGTYCPGMDPSPPPEYITCLGD--LY 273

Query: 239 FLEVLKDSKCRPENGEVTLLQKSF 262
            +  ++DS+    N +   +++ +
Sbjct: 274 SVAWMEDSETH--NLKRESVKQQY 295
>pir||T10944 cysteine proteinase (EC 3.4.22.-) precursor - spring vetch
 emb|CAA07639.1| (AJ007743) cysteine proteinase precursor [Vicia sativa]
          Length = 503

 Score =  394 bits (1002), Expect = e-108
 Identities = 59/264 (22%), Positives = 103/264 (38%), Gaps = 19/264 (7%)

Query: 9   VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
           V  L   P  +          WAV+++ S  + N RH  +    Y L  K G+ +++I+V
Sbjct: 48  VIRLPGEPV-DADVEDEIGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVV 106

Query: 69  FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
           F+ D  A     P P  I   P   + PN+Y   +  DY    V       V+ G   + 
Sbjct: 107 FMYDDIAYSEFNPRPGVIINHP---QGPNVY-DGVPKDYTGDFVTADNLYAVILGDKSKV 162

Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
              S + +++     I IY + H         +  ++ + D  D L      R Y+++V 
Sbjct: 163 RGGSGKVINSKAEDRIFIYYSDHGGPGVLGMPNMPYVYAMDFIDVLKKKHASRGYQQMVI 222

Query: 188 LVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL-------DPPSGFTVITRWTFE 238
            V+ C + S+   I     +V    +S A   S+  +L        PP   T +      
Sbjct: 223 YVEACESGSVFQGIMPKDIDVYVTTASNAEESSWGTYLVPGVYPASPPEYITCLGD--LY 280

Query: 239 FLEVLKDSKCRPENGEVTLLQKSF 262
            +  ++DS+    N +   L++ F
Sbjct: 281 SVAWMEDSETH--NLKRETLKQQF 302
>gb|AAF89679.1| (AF169973) asparaginyl endopeptidase [Sesamum indicum]
          Length = 489

 Score =  393 bits (1001), Expect = e-108
 Identities = 51/258 (19%), Positives = 98/258 (37%), Gaps = 18/258 (6%)

Query: 14  LAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDS 73
             P        N    WAV+++ S  F N RH  +    Y + +K G+ D++I+VF+ D 
Sbjct: 40  RWPLDRRETEDNA-TRWAVLVAGSNGFGNYRHQADVCHAYQILKKGGLRDENIIVFMYDD 98

Query: 74  YACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS- 132
            A +   P    I   P      ++Y   +  DY    V       V+ G        S 
Sbjct: 99  IAMNELNPRKGVIINHPT---GGDVYA-GVPKDYTGEQVTAENLYAVILGDKSAIKGGSG 154

Query: 133 RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTC 192
           + +D+  N  I IY + H         +  ++ + D  + L        Y+++V  V+ C
Sbjct: 155 KVVDSKPNDRIFIYYSDHGGPGVLGMPNMPYLYANDFIEVLKKKHASGTYKEMVIYVEAC 214

Query: 193 RALSLCLEIKA--PNVVCLASSEAHLDSYSHHLD------PPSGFTVITRWTFEFLEVLK 244
            + S+   +     ++    +S A   S+  +        PP   T +       +  ++
Sbjct: 215 ESGSVFEGLMPDDLDIYVTTASNAEESSWGTYCPGMDPPPPPEYITCLGD--LYSVAWME 272

Query: 245 DSKCRPENGEVTLLQKSF 262
           DS+    N +   +++ +
Sbjct: 273 DSESH--NLKRETVEQQY 288
>pir||S60049 vacuolar processing enzyme (EC 3.4.22.-) isozyme alpha precursor -
           Arabidopsis thaliana
          Length = 478

 Score =  392 bits (998), Expect = e-108
 Identities = 56/262 (21%), Positives = 105/262 (39%), Gaps = 21/262 (8%)

Query: 10  ANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVF 69
           A+ F  P          +  WAV+++ S  ++N RH  +    Y   +K G+ +++I+V 
Sbjct: 29  ASKFFRPTENDDD----STKWAVLVAGSSGYWNYRHQADVCHAYQRLKKGGVKEENIVVL 84

Query: 70  LSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENT 129
           + D  A +   P P  I  +P      ++Y   +  DY   +V V   L V+ G      
Sbjct: 85  MYDDIAENEENPRPGVIINSPN---GEDVY-NGVPKDYTGDEVNVDNLLAVILGNKTALK 140

Query: 130 PPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFL 188
             S + +D+  N +I IY + H          S  + + D+ D L   +    Y+ LVF 
Sbjct: 141 GGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFY 200

Query: 189 VDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFL 240
           ++ C + S+   +     N+    +S A   S+  +        P    T +       +
Sbjct: 201 LEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGD--LYSV 258

Query: 241 EVLKDSKCRPENGEVTLLQKSF 262
             ++DS+    N +   L + +
Sbjct: 259 AWIEDSEKH--NLQTETLHEQY 278
>gb|AAF99765.1|AC003981_15 (AC003981) F22O13.24 [Arabidopsis thaliana]
          Length = 407

 Score =  389 bits (990), Expect = e-107
 Identities = 91/278 (32%), Positives = 154/278 (54%), Gaps = 23/278 (8%)

Query: 5   LLWLVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDD 64
           +L LV  L  +  +        TN WAV++ +SR++                ++ G+ D+
Sbjct: 3   ILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFW--------------TVKRLGIPDE 48

Query: 65  HILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGR 124
            I++ L+D  AC+ R   PA ++    +  + NLYG N+ VDY  Y+V V  FL VL GR
Sbjct: 49  RIILMLADDMACNARNEYPAQVFN--NENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGR 106

Query: 125 YDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRK 184
           ++   P S+RL +DE S+I++Y  GH  ++F KFQD+E + S D+AD +  M E+RR+++
Sbjct: 107 HENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKE 166

Query: 185 LVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVLK 244
           L+ +VDTC+A +L  ++++P V+ + SS    +SYSHHLD   G +V+ R+T+  L   +
Sbjct: 167 LMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFE 226

Query: 245 ------DSKCRPENGEVTLLQKSFYDFN-YGPERLSLP 275
                 ++          L+  ++Y  + Y P  + +P
Sbjct: 227 RLNIYDNASLNRSYDPRLLMSTAYYRTDLYQPHLVEVP 264
>pir||T00731 hypothetical protein F22O13.26 - Arabidopsis thaliana
          Length = 428

 Score =  386 bits (983), Expect = e-106
 Identities = 93/285 (32%), Positives = 157/285 (54%), Gaps = 16/285 (5%)

Query: 5   LLWLVANLFLAPAAEGFHGMNKTNTWAVILSSSRY----FFNLRHTTNALAMYHLCRKHG 60
           +L LV  L  +  +        TN WAV++ +SR+       L    + L +    ++ G
Sbjct: 3   ILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFCSLHSLVLTFIFSLLGVSRTVKRLG 62

Query: 61  MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120
           + D+ I++ L+D  AC+ R   PA ++    +  + NLYG N+ VDY  Y+V V  FL V
Sbjct: 63  IPDERIILMLADDMACNARNEYPAQVFN--NENHKLNLYGDNVEVDYRGYEVTVENFLRV 120

Query: 121 LQGRYDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQR 180
           L GR++   P S+RL +DE S+I++Y  GH  ++F KFQD+E + S D+AD +  M E+R
Sbjct: 121 LTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 180

Query: 181 RYRKLVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFL 240
           R+++L+ +VDTC+A +L  ++++P V+ + SS    +SYSHHLD   G +V+ R+T+  L
Sbjct: 181 RFKELMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTL 240

Query: 241 EVLK------DSKCR---PENGEVTLLQKSFYDFN-YGPERLSLP 275
              +      ++             L+  ++Y  + Y P  + +P
Sbjct: 241 AFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVP 285
>dbj|BAB29018.1| (AK013853) data source:SPTR, source key:O14822,
           evidence:ISS~homolog to GPI TRANSAMIDASE~putative [Mus
           musculus]
          Length = 395

 Score =  381 bits (971), Expect = e-105
 Identities = 96/263 (36%), Positives = 150/263 (56%), Gaps = 11/263 (4%)

Query: 13  FLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSD 72
            +   AE F     TN WAV++ +SR++FN RH  N L++Y   ++ G+ D HI++ L+D
Sbjct: 29  HIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVLMLAD 88

Query: 73  SYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS 132
             AC+ R P PAT++    +  + N+YG ++ VDY SY   V  FL VL      +TP S
Sbjct: 89  DMACNARNPKPATVFSH--KNMELNVYGDDVEVDYRSYRGTVENFLRVLTXEVPPSTPRS 146

Query: 133 RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTC 192
           +RL +D+ SNI+IY  GH    F KFQDSE +++ ++AD    MW++RRY +L+F++DTC
Sbjct: 147 KRLLSDDKSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTC 206

Query: 193 RALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVLKD----SKC 248
           +  S+     +PN++ LASS+   DS SH  DP  G  ++ R+TF  LE  K+    S+ 
Sbjct: 207 QGASMYERFYSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFWKEINPASQT 266

Query: 249 RPENG-----EVTLLQKSFYDFN 266
              +      +   +    +  +
Sbjct: 267 NMNDLFQVCPKSLCVSTPGHRTD 289
>gb|AAF73260.1|AF154349_1 (AF154349) legumain [Rattus norvegicus]
          Length = 435

 Score =  375 bits (955), Expect = e-103
 Identities = 60/268 (22%), Positives = 113/268 (41%), Gaps = 19/268 (7%)

Query: 5   LLWLVANLFLAPAAEG------FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRK 58
           ++W VA L       G          +    W VI++ S  ++N RH  +A   Y +  +
Sbjct: 1   MIWKVAVLLSLVLGAGAVHIGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHR 60

Query: 59  HGMDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFL 118
           +G+ D+ I+V + D  A +   P P  +   P      ++Y   +  DY   DV    FL
Sbjct: 61  NGIPDEQIIVMMYDDIANNEENPTPGVVINRPN---GTDVYK-GVPKDYTGEDVTPENFL 116

Query: 119 GVLQGRYDEN--TPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMM 176
            VL+G  +        + L +    ++ +Y   H A     F + E +   D+  T+  M
Sbjct: 117 AVLRGDEEAVKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNKTIRYM 175

Query: 177 WEQRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRW 235
           +E + Y+K+VF ++ C + S+   +    +V    ++  +  SY+ + D     T +  W
Sbjct: 176 YEHKMYQKMVFYIEACESGSMMNHLPDDIDVYATTAANPNESSYACYYDEER-STYLGDW 234

Query: 236 TFEFLEVLKDSKCRPENGEVTLLQKSFY 263
               +  ++DS    E+     L K ++
Sbjct: 235 --YSVNWMEDS--DVEDLTKETLHKQYH 258
>ref|NP_071562.1| (NM_022226) protease, cysteine, 1 (legumain) [Rattus norvegicus]
 dbj|BAA84750.1| (AB032766) legumain [Rattus norvegicus]
          Length = 435

 Score =  374 bits (952), Expect = e-102
 Identities = 60/268 (22%), Positives = 112/268 (41%), Gaps = 19/268 (7%)

Query: 5   LLWLVANLFLAPAAEG------FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRK 58
           + W VA L       G          +    W VI++ S  ++N RH  +A   Y +  +
Sbjct: 1   MTWRVAVLLSLVLGAGAVHIGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHR 60

Query: 59  HGMDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFL 118
           +G+ D+ I+V + D  A +   P P  +   P      ++Y   +  DY   DV    FL
Sbjct: 61  NGIPDEQIIVMMYDDIANNEENPTPGVVINRPN---GTDVYK-GVPKDYTGEDVTPENFL 116

Query: 119 GVLQGRYDEN--TPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMM 176
            VL+G  +        + L +    ++ +Y   H A     F + E +   D+  T+  M
Sbjct: 117 AVLRGDEEAVKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNKTIRYM 175

Query: 177 WEQRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRW 235
           +E + Y+K+VF ++ C + S+   +    +V    ++  +  SY+ + D     T +  W
Sbjct: 176 YEHKMYQKMVFYIEACESGSMMNHLPDDIDVYATTAANPNESSYACYYDEER-STYLGDW 234

Query: 236 TFEFLEVLKDSKCRPENGEVTLLQKSFY 263
               +  ++DS    E+     L K ++
Sbjct: 235 --YSVNWMEDS--DVEDLTKETLHKQYH 258
>ref|NP_035305.1| (NM_011175) protease, cysteine, 1; preprolegumain [Mus musculus]
 emb|CAA04439.1| (AJ000990) legumain [Mus musculus]
 gb|AAF21659.1|AF044266_1 (AF044266) preprolegumain; cysteine Protease [Mus musculus]
          Length = 435

 Score =  373 bits (948), Expect = e-102
 Identities = 62/268 (23%), Positives = 111/268 (41%), Gaps = 19/268 (7%)

Query: 5   LLWLVANLFLAPAAEG------FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRK 58
           + W VA L       G          +    W VI++ S  ++N RH  +A   Y +  +
Sbjct: 1   MTWRVAVLLSLVLGAGAVPVGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHR 60

Query: 59  HGMDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFL 118
           +G+ D+ I+V + D  A     P P  +   P      ++Y   +  DY   DV    FL
Sbjct: 61  NGIPDEQIIVMMYDDIANSEENPTPGVVINRPN---GTDVYK-GVLKDYTGEDVTPENFL 116

Query: 119 GVLQGRYDEN--TPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMM 176
            VL+G  +        + L +    ++ IY   H A     F + + +   D+  T+  M
Sbjct: 117 AVLRGDAEAVKGKGSGKVLKSGPRDHVFIYFTDHGATGILVFPN-DDLHVKDLNKTIRYM 175

Query: 177 WEQRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRW 235
           +E + Y+K+VF ++ C + S+   +    NV    ++     SY+ + D   G T +  W
Sbjct: 176 YEHKMYQKMVFYIEACESGSMMNHLPDDINVYATTAANPKESSYACYYDEERG-TYLGDW 234

Query: 236 TFEFLEVLKDSKCRPENGEVTLLQKSFY 263
               +  ++DS    E+     L K ++
Sbjct: 235 --YSVNWMEDS--DVEDLTKETLHKQYH 258
>dbj|BAA09530.1| (D55696) cysteine protease [Homo sapiens]
          Length = 433

 Score =  370 bits (942), Expect = e-101
 Identities = 61/266 (22%), Positives = 113/266 (41%), Gaps = 17/266 (6%)

Query: 5   LLWLVANLFLAPAAEG----FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG 60
           ++W VA         G        +    WAVI++ S  ++N RH  +A   Y +  ++G
Sbjct: 1   MVWKVAVFLSVALGIGAVPIDDPEDGGKHWAVIVAGSNGWYNYRHQADACHAYQIIHRNG 60

Query: 61  MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120
           + D+ I+V + D  A     P P  +   P      ++Y   +  DY   DV  + FL V
Sbjct: 61  IPDEQIVVMMYDDIAYSEDNPTPGIVINRPN---GTDVYQ-GVPKDYTGEDVTPQNFLAV 116

Query: 121 LQGRYDENT--PPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWE 178
           L+G  +        + L +    ++ IY   H +     F + E +   D+ +T+  M++
Sbjct: 117 LRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYK 175

Query: 179 QRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRWTF 237
            + YRK+VF ++ C + S+   +    NV    ++     SY+ + D     T +  W  
Sbjct: 176 HKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKR-STYLGDW-- 232

Query: 238 EFLEVLKDSKCRPENGEVTLLQKSFY 263
             +  ++DS    E+     L K ++
Sbjct: 233 YSVNWMEDS--DVEDLTKETLHKQYH 256
>ref|XP_007399.4| (XM_007399) legumain [Homo sapiens]
          Length = 401

 Score =  370 bits (941), Expect = e-101
 Identities = 60/266 (22%), Positives = 112/266 (41%), Gaps = 17/266 (6%)

Query: 5   LLWLVANLFLAPAAEG----FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG 60
           ++W VA         G        +    W VI++ S  ++N RH  +A   Y +  ++G
Sbjct: 1   MVWKVAVFLSVALGIGAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNG 60

Query: 61  MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120
           + D+ I+V + D  A     P P  +   P      ++Y   +  DY   DV  + FL V
Sbjct: 61  IPDEQIVVMMYDDIAYSEDNPTPGIVINRPN---GTDVYQ-GVPKDYTGEDVTPQNFLAV 116

Query: 121 LQGRYDENT--PPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWE 178
           L+G  +        + L +    ++ IY   H +     F + E +   D+ +T+  M++
Sbjct: 117 LRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYK 175

Query: 179 QRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRWTF 237
            + YRK+VF ++ C + S+   +    NV    ++     SY+ + D     T +  W  
Sbjct: 176 HKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKR-STYLGDW-- 232

Query: 238 EFLEVLKDSKCRPENGEVTLLQKSFY 263
             +  ++DS    E+     L K ++
Sbjct: 233 YSVNWMEDS--DVEDLTKETLHKQYH 256
>gb|AAH03061.1|AAH03061 (BC003061) protease, cysteine, 1 (legumain) [Homo sapiens]
          Length = 433

 Score =  368 bits (937), Expect = e-101
 Identities = 60/266 (22%), Positives = 112/266 (41%), Gaps = 17/266 (6%)

Query: 5   LLWLVANLFLAPAAEG----FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG 60
           ++W VA         G        +    W VI++ S  ++N RH  +A   Y +  ++G
Sbjct: 1   MVWKVAVFLSVALGIGAIPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNG 60

Query: 61  MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120
           + D+ I+V + D  A     P P  +   P      ++Y   +  DY   DV  + FL V
Sbjct: 61  IPDEQIVVMMYDDIAYSEDNPTPGIVINRPN---GTDVYQ-GVPKDYTGEDVTPQNFLAV 116

Query: 121 LQGRYDENT--PPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWE 178
           L+G  +        + L +    ++ IY   H +     F + E +   D+ +T+  M++
Sbjct: 117 LRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYK 175

Query: 179 QRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRWTF 237
            + YRK+VF ++ C + S+   +    NV    ++     SY+ + D     T +  W  
Sbjct: 176 HKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKR-STYLGDW-- 232

Query: 238 EFLEVLKDSKCRPENGEVTLLQKSFY 263
             +  ++DS    E+     L K ++
Sbjct: 233 YSVNWMEDS--DVEDLTKETLHKQYH 256
>emb|CAB51545.1| (AJ243876) vacuolar processing enzyme [Lycopersicon esculentum]
          Length = 460

 Score =  368 bits (937), Expect = e-101
 Identities = 60/262 (22%), Positives = 110/262 (41%), Gaps = 24/262 (9%)

Query: 19  EGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDSYACDP 78
           +  +  +    WAV+++ S+ ++N RH  N    Y L +K G+ D+HI+VF+ D  A +P
Sbjct: 29  DEDYEDSIGTKWAVLVAGSKEWYNYRHQANLCHAYQLLKKGGLKDEHIIVFMYDDIANNP 88

Query: 79  RKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS-RRLDT 137
             P P  I   P      ++Y   +  DY   D   + F  V+ G     T  S + +++
Sbjct: 89  ENPRPGVIINNP---HGHDVYK-GVPKDYTGKDCNAQNFYSVILGNKSALTGGSGKVVNS 144

Query: 138 DENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTCRALSL 197
             N  I IY   H A       +   + + D+ + L      R Y+K+VF ++ C + S+
Sbjct: 145 GPNDYIFIYYTDHGAPGLVGMPEDPPVYAIDLNEVLKKKHASRTYKKMVFYLEACDSGSM 204

Query: 198 CLEIKA--PNVVCLASSEAHLDSYSHHL-----------DPPSGF--TVITRWTFEFLEV 242
             ++     N+    SS+   D ++ +             PP  F    +       +  
Sbjct: 205 FADLLDEGLNIYATTSSKPDEDGWATYCYFTGDTSCYGECPPKDFKDNCLGD--LFSVSW 262

Query: 243 LKDSKCRPENGEVTLLQKSFYD 264
           L++S     + +V  L+K +  
Sbjct: 263 LENSDLH--DLQVETLEKQYLR 282
>ref|NP_005597.1| (NM_005606) legumain; protease, cysteine, 1 (legumain) [Homo
           sapiens]
 sp|Q99538|LEGU_HUMAN Legumain precursor (Asparaginyl endopeptidase)
 emb|CAA70989.1| (Y09862) legumain [Homo sapiens]
 gb|AAH08004.1|AAH08004 (BC008004) protease, cysteine, 1 (legumain) [Homo sapiens]
          Length = 433

 Score =  368 bits (936), Expect = e-101
 Identities = 60/266 (22%), Positives = 112/266 (41%), Gaps = 17/266 (6%)

Query: 5   LLWLVANLFLAPAAEG----FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG 60
           ++W VA         G        +    W VI++ S  ++N RH  +A   Y +  ++G
Sbjct: 1   MVWKVAVFLSVALGIGAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNG 60

Query: 61  MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120
           + D+ I+V + D  A     P P  +   P      ++Y   +  DY   DV  + FL V
Sbjct: 61  IPDEQIVVMMYDDIAYSEDNPTPGIVINRPN---GTDVYQ-GVPKDYTGEDVTPQNFLAV 116

Query: 121 LQGRYDENT--PPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWE 178
           L+G  +        + L +    ++ IY   H +     F + E +   D+ +T+  M++
Sbjct: 117 LRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYK 175

Query: 179 QRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRWTF 237
            + YRK+VF ++ C + S+   +    NV    ++     SY+ + D     T +  W  
Sbjct: 176 HKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKR-STYLGDW-- 232

Query: 238 EFLEVLKDSKCRPENGEVTLLQKSFY 263
             +  ++DS    E+     L K ++
Sbjct: 233 YSVNWMEDS--DVEDLTKETLHKQYH 256
>pir||T40853 probable cysteine proteinase (EC 3.4.22.-) [similarity] - fission
           yeast (Schizosaccharomyces pombe)
 emb|CAB57844.1| (AL121783) putative gpi-anchor transamidase [Schizosaccharomyces
           pombe]
 emb|CAC13970.1| (AJ250428) GPI8p transamidase [Schizosaccharomyces pombe]
          Length = 380

 Score =  367 bits (934), Expect = e-100
 Identities = 107/269 (39%), Positives = 159/269 (58%), Gaps = 7/269 (2%)

Query: 4   MLLWLVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDD 63
           M++  VA L L          + TN WAV++S+SR++FN RHT N L +Y   ++ G+ D
Sbjct: 1   MIVQFVALLLLNLLQIIAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPD 60

Query: 64  DHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQG 123
             I++ ++D YAC+ R   P T++    +A   +LYG  I +DY  Y+V V  F+ +L  
Sbjct: 61  SQIILMIADDYACNSRNLFPGTVFDNADRA--LDLYGEEIEIDYKGYEVTVEAFIRLLTE 118

Query: 124 RYDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYR 183
           R  ENTP S+RL T+E SNI+IY  GH  + F KFQD+E +SS D+AD +  + + +RY 
Sbjct: 119 RVPENTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYN 178

Query: 184 KLVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVL 243
           +++F+VDTC+A SL  +I +PNV+ + SSE    SYSHH D   G  VI R+TF  LE L
Sbjct: 179 EILFMVDTCQANSLYTKIYSPNVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSNLEFL 238

Query: 244 KDSKCRPENGEVTLLQKSFYDFNYGPERL 272
           ++   R ++     +Q       Y P  +
Sbjct: 239 EN---RVDSKSKLTMQDLINS--YNPYEI 262
>dbj|BAB69947.1| (AB060129) legumain [Bos taurus]
          Length = 433

 Score =  365 bits (930), Expect = e-100
 Identities = 59/265 (22%), Positives = 110/265 (41%), Gaps = 17/265 (6%)

Query: 5   LLWLVANLFLAPAAEGF----HGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG 60
           ++W    L       G        +    W VI++ S  ++N RH  +A   Y +  ++G
Sbjct: 1   MIWEFTVLLSLVLGTGAVPLEDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIVHRNG 60

Query: 61  MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120
           + D+ I+V + D  A     P P  +   P      ++Y   +  DY   DV  + FL V
Sbjct: 61  IPDEQIIVMMYDDIANSEDNPTPGIVINRPN---GSDVYQ-GVLKDYTGEDVTPKNFLAV 116

Query: 121 LQGRYDENT--PPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWE 178
           L+G  +        + L +    ++ +Y   H A     F + E +   D+ +T+  M+E
Sbjct: 117 LRGDAEAVKGVGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNETIRYMYE 175

Query: 179 QRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRWTF 237
            + Y+K+VF ++ C + S+   +    NV    ++     SY+ + D     T +  W  
Sbjct: 176 HKMYQKMVFYIEACESGSMMNHLPPDINVYATTAANPRESSYACYYDEQR-STFLGDW-- 232

Query: 238 EFLEVLKDSKCRPENGEVTLLQKSF 262
             +  ++DS    E+     L K +
Sbjct: 233 YSVNWMEDS--DVEDLTKETLHKQY 255
>gb|AAF45703.1| (AE003422) EG:133E12.3 gene product [Drosophila melanogaster]
          Length = 322

 Score =  363 bits (923), Expect = 3e-99
 Identities = 91/226 (40%), Positives = 140/226 (61%), Gaps = 2/226 (0%)

Query: 19  EGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDSYACDP 78
           +       TN WAV++ +SR++FN RH  N L++Y   ++ G+ D  I++ ++D  AC+ 
Sbjct: 32  DAAQRSTHTNNWAVLVDASRFWFNYRHVANVLSIYRSVKRLGIPDSQIILMIADDMACNA 91

Query: 79  RKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPSRRLDTD 138
           R P P  +Y      +  N+YG ++ VDY  Y+V V  F+ +L GR    T  S++L +D
Sbjct: 92  RNPRPGQVYNNAN--QHINVYGDDVEVDYRGYEVTVENFVRLLTGRTQNGTARSKKLLSD 149

Query: 139 ENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTCRALSLC 198
             SN++IY  GH  + F KFQDSE ++S ++AD +  MWE++RY +L F+VDTC+A SL 
Sbjct: 150 AGSNVLIYLTGHGGDGFLKFQDSEEITSQELADGIQQMWEKKRYNELFFMVDTCQAASLY 209

Query: 199 LEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVLK 244
            +  +PNV+ +ASS    DS SHH+DP  G  +I R+T+  LE L+
Sbjct: 210 EKFTSPNVLAVASSLVGEDSLSHHVDPSIGVYMIDRYTYYALEFLE 255
>ref|NP_188656.1| (NM_112912) vacuolar processing enzyme/asparaginyl endopeptidase,
           putative [Arabidopsis thaliana]
 dbj|BAB01880.1| (AP000383) vacuolar processing enzyme (proteinase) [Arabidopsis
           thaliana]
          Length = 466

 Score =  358 bits (910), Expect = 9e-98
 Identities = 58/254 (22%), Positives = 104/254 (40%), Gaps = 17/254 (6%)

Query: 19  EGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDSYACDP 78
           E      K   WAV+++ S  ++N RH  +    Y + RK G+ D++I+VF+ D  A   
Sbjct: 36  ESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAYQILRKGGLKDENIIVFMYDDIAFSS 95

Query: 79  RKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPP-SRRLDT 137
             P P  I   P      ++Y   +  DY    V V+ F  VL G     T    + + +
Sbjct: 96  ENPRPGVIINKPD---GEDVYK-GVPKDYTKEAVNVQNFYNVLLGNESGVTGGNGKVVKS 151

Query: 138 DENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTCRALSL 197
             N NI IY A H A         + + + D  + L  M ++++Y K+V  V+ C + S+
Sbjct: 152 GPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMHKRKKYNKMVIYVEACESGSM 211

Query: 198 CLEIKA--PNVVCLASSEAHLDSYSHHLDPPSGF------TVITRWTFEFLEVLKDSKCR 249
              I     N+  + ++ +   S+  +             T +       +  L+DS   
Sbjct: 212 FEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEIGTCLGD--TFSISWLEDSDLH 269

Query: 250 PENGEVTLLQKSFY 263
             +     L++ ++
Sbjct: 270 --DMSKETLEQQYH 281
>ref|NP_010618.1| (NC_001136) Protein involved in the attachment of
           glycosylphosphatidylinositol (GPI) anchors to proteins;
           Gpi8p [Saccharomyces cerevisiae]
 sp|P49018|GPI8_YEAST GPI-ANCHOR TRANSAMIDASE
 pir||S59796 probable membrane protein YDR331w - yeast (Saccharomyces
           cerevisiae)
 gb|AAB64766.1| (U32517) Ydr331wp [Saccharomyces cerevisiae]
          Length = 411

 Score =  357 bits (909), Expect = 1e-97
 Identities = 104/270 (38%), Positives = 158/270 (58%), Gaps = 10/270 (3%)

Query: 2   LPMLLWLVANLFLAPA--AEGFHGMNK--TNTWAVILSSSRYFFNLRHTTNALAMYHLCR 57
           LP+LL  +  L L+ A   +  H +    TN WAV++S+SR++FN RH  N L+MY   +
Sbjct: 7   LPLLLLYIFLLPLSGANNTDAAHEVIATNTNNWAVLVSTSRFWFNYRHMANVLSMYRTVK 66

Query: 58  KHGMDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRF 117
           + G+ D  I++ LSD  AC+ R   P +++    +    +LYG ++ VDY  Y+V V  F
Sbjct: 67  RLGIPDSQIILMLSDDVACNSRNLFPGSVFN--NKDHAIDLYGDSVEVDYRGYEVTVENF 124

Query: 118 LGVLQGRYDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMW 177
           + +L  R+ E+ P S+RL TDENSNI IY  GH  + F KFQD+E ++S DIAD    M+
Sbjct: 125 IRLLTDRWTEDHPKSKRLLTDENSNIFIYMTGHGGDDFLKFQDAEEIASEDIADAFQQMY 184

Query: 178 EQRRYRKLVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTF 237
           E++RY ++ F++DTC+A ++  +  +PN++ + SSE    SYSHH D   G  VI R+T+
Sbjct: 185 EKKRYNEIFFMIDTCQANTMYSKFYSPNILAVGSSEMDESSYSHHSDVEIGVAVIDRFTY 244

Query: 238 EFLEVLKDSKCRPENGEVTLLQKSFYDFNY 267
             L+ L+             LQ  F  F +
Sbjct: 245 YCLDFLEQIDKN----STLTLQDLFDSFTF 270
>emb|CAB42651.1| (AJ238881) putative preprolegumain [Nicotiana tabacum]
          Length = 419

 Score =  353 bits (898), Expect = 2e-96
 Identities = 43/227 (18%), Positives = 86/227 (36%), Gaps = 17/227 (7%)

Query: 45  HTTNALAMYHLCRKHGMDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIR 104
           H  +    Y + ++ G+ D++I+VF+ D  A     P P  I   P      ++Y   + 
Sbjct: 1   HQADVCHAYQILKRGGLKDENIVVFMYDDIAKSELNPRPGVIINHPN---GSDVYA-GVP 56

Query: 105 VDYASYDVGVRRFLGVLQGRYDENTPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEF 163
            DY    V       VL G        S + +D+  N  I +Y + H         +  F
Sbjct: 57  KDYTGEHVTAANLYAVLLGDKSAVKGGSGKIVDSKPNDRIFLYYSDHGGPGVLGMPNMPF 116

Query: 164 MSSTDIADTLMMMWEQRRYRKLVFLVDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSH 221
           + + D  + L        Y+++V  ++ C + S+   +     N+    +S A   S+  
Sbjct: 117 LYAKDFIEVLKKKHAAGTYKEMVLYIEACESGSVFEGMMPEDLNIYVTTASNAEESSWGT 176

Query: 222 HLD------PPSGFTVITRWTFEFLEVLKDSKCRPENGEVTLLQKSF 262
           +        PP   T +       +  ++DS+    N +   +++ +
Sbjct: 177 YCPGMDPPPPPEYITCLGD--LYSVAWMEDSESH--NLKKETIKQQY 219
CPU time:    92.70 user secs.	    2.28 sys. secs	   94.98 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.314    0.179    0.583 

Gapped
Lambda     K      H
   0.270   0.0629    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 255118333
Number of Sequences: 887402
Number of extensions: 14913996
Number of successful extensions: 27949
Number of sequences better than 10.0: 77
Number of HSP's better than 10.0 without gapping: 71
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 27541
Number of HSP's gapped (non-prelim): 83
length of query: 319
length of database: 277,845,442
effective HSP length: 52
effective length of query: 267
effective length of database: 231,700,538
effective search space: 61864043646
effective search space used: 61864043646
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.0 bits)
S2: 74 (32.8 bits)