Sequences with E-value BETTER than threshold (0.002)
emb|CAC67556.1| (AJ308106) Gpi8 transamidase [Trypanosoma brucei] 492 e-138
emb|CAB55340.1| (AJ242865) GPI:protein transamidase [Leishmania ... 431 e-120
ref|NP_563825.1| (NM_100749) putative GPI-anchor transamidase (U... 418 e-116
pir||T12043 probable legumain (EC 3.4.22.34) precursor - kidney ... 410 e-113
emb|CAC18099.1| (AJ251453) putative legumain [Zea mays] 409 e-113
emb|CAB64544.1| (AJ131718) legumain-like protease [Zea mays] 408 e-113
dbj|BAA76744.1| (D89971) asparaginyl endopeptidase (VmPE-1) [Vig... 408 e-112
gb|AAK15049.1|AF238384_1 (AF238384) asparaginyl endopeptidase [V... 407 e-112
emb|CAB64545.1| (AJ131719) legumain-like protease [Zea mays] 406 e-112
sp|P49043|VPE_CITSI VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) >... 406 e-112
sp|P49045|VPE_SOYBN VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) >... 405 e-112
sp|P49046|LEGU_CANEN Legumain precursor (Asparaginyl endopeptida... 405 e-112
emb|CAB16318.1| (Z99174) cysteine proteinase precursor [Vicia na... 404 e-112
gb|AAF69014.1|AF260827_1 (AF260827) cysteine protease [Ipomoea b... 404 e-111
dbj|BAA76745.1| (D89972) asparaginyl endopeptidase (VmPE-1A) [Vi... 403 e-111
dbj|BAA84650.1| (AB025310) asparaginyl endopeptidase [Oryza sati... 402 e-111
sp|P49042|VPE_RICCO Vacuolar processing enzyme precursor (VPE) >... 401 e-111
gb|AAD04883.1| (AF082347) C13 endopeptidase NP1 precursor [Zea m... 401 e-111
pir||T12044 probable legumain (EC 3.4.22.34) precursor - kidney ... 401 e-110
pir||S60050 vacuolar processing enzyme (EC 3.4.22.-) isozyme bet... 400 e-110
emb|CAC18100.1| (AJ251454) putative legumain [Zea mays] 400 e-110
emb|CAC43295.1| (AJ309173) putative vacuolar processing enzyme [... 399 e-110
ref|NP_195020.1| (NM_119448) gamma-VPE (vacuolar processing enzy... 399 e-110
ref|NP_176458.1| (NM_104948) beta-VPE [Arabidopsis thaliana] >gi... 399 e-110
sp|Q39119|VPEG_ARATH VACUOLAR PROCESSING ENZYME, GAMMA-ISOZYME P... 399 e-110
sp|P49044|VPE_VICSA VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) (... 398 e-110
ref|NP_180165.1| (NM_128154) putative vacuolar processing enzyme... 397 e-110
ref|XP_039644.1| (XM_039644) phosphatidylinositol glycan, class ... 397 e-109
emb|CAA68871.1| (Y07596) gpi8 [Homo sapiens] 397 e-109
gb|AAF89646.1|AF169019_1 (AF169019) seed maturation protein PM40... 397 e-109
pir||T10944 cysteine proteinase (EC 3.4.22.-) precursor - spring... 394 e-108
gb|AAF89679.1| (AF169973) asparaginyl endopeptidase [Sesamum ind... 393 e-108
pir||S60049 vacuolar processing enzyme (EC 3.4.22.-) isozyme alp... 392 e-108
gb|AAF99765.1|AC003981_15 (AC003981) F22O13.24 [Arabidopsis thal... 389 e-107
pir||T00731 hypothetical protein F22O13.26 - Arabidopsis thaliana 386 e-106
dbj|BAB29018.1| (AK013853) data source:SPTR, source key:O14822, ... 381 e-105
gb|AAF73260.1|AF154349_1 (AF154349) legumain [Rattus norvegicus] 375 e-103
ref|NP_071562.1| (NM_022226) protease, cysteine, 1 (legumain) [R... 374 e-102
ref|NP_035305.1| (NM_011175) protease, cysteine, 1; preproleguma... 373 e-102
dbj|BAA09530.1| (D55696) cysteine protease [Homo sapiens] 370 e-101
ref|XP_007399.4| (XM_007399) legumain [Homo sapiens] 370 e-101
gb|AAH03061.1|AAH03061 (BC003061) protease, cysteine, 1 (legumai... 368 e-101
emb|CAB51545.1| (AJ243876) vacuolar processing enzyme [Lycopersi... 368 e-101
ref|NP_005597.1| (NM_005606) legumain; protease, cysteine, 1 (le... 368 e-101
pir||T40853 probable cysteine proteinase (EC 3.4.22.-) [similari... 367 e-100
dbj|BAB69947.1| (AB060129) legumain [Bos taurus] 365 e-100
gb|AAF45703.1| (AE003422) EG:133E12.3 gene product [Drosophila m... 363 3e-99
ref|NP_188656.1| (NM_112912) vacuolar processing enzyme/asparagi... 358 9e-98
ref|NP_010618.1| (NC_001136) Protein involved in the attachment ... 357 1e-97
emb|CAB42651.1| (AJ238881) putative preprolegumain [Nicotiana ta... 353 2e-96
Alignments
>emb|CAC67556.1| (AJ308106) Gpi8 transamidase [Trypanosoma brucei]
Length = 319
Score = 492 bits (1254), Expect = e-138
Identities = 319/319 (100%), Positives = 319/319 (100%)
Query: 1 MLPMLLWLVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG 60
MLPMLLWLVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG
Sbjct: 1 MLPMLLWLVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG 60
Query: 61 MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120
MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV
Sbjct: 61 MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120
Query: 121 LQGRYDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQR 180
LQGRYDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQR
Sbjct: 121 LQGRYDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQR 180
Query: 181 RYRKLVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFL 240
RYRKLVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFL
Sbjct: 181 RYRKLVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFL 240
Query: 241 EVLKDSKCRPENGEVTLLQKSFYDFNYGPERLSLPQPLSEPAHFDAVNRPNAIREWKMDE 300
EVLKDSKCRPENGEVTLLQKSFYDFNYGPERLSLPQPLSEPAHFDAVNRPNAIREWKMDE
Sbjct: 241 EVLKDSKCRPENGEVTLLQKSFYDFNYGPERLSLPQPLSEPAHFDAVNRPNAIREWKMDE 300
Query: 301 FFCEQDRDKIPVELRYDLF 319
FFCEQDRDKIPVELRYDLF
Sbjct: 301 FFCEQDRDKIPVELRYDLF 319
>emb|CAB55340.1| (AJ242865) GPI:protein transamidase [Leishmania mexicana]
Length = 349
Score = 431 bits (1098), Expect = e-120
Identities = 170/316 (53%), Positives = 220/316 (68%), Gaps = 12/316 (3%)
Query: 15 APAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDSY 74
A A G G ++N WAVI+SSSRY FN RHT NAL MYHL R+HG+DDDHIL+FLSDS+
Sbjct: 33 ASAPLGATGKGQSNNWAVIVSSSRYLFNYRHTANALTMYHLLRQHGIDDDHILLFLSDSF 92
Query: 75 ACDPRKPNPATIYGAPAQAEQPN------LYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
ACDPR PA I+ P A + LYGC+ +VDYA DV VRRFL VLQGRYDEN
Sbjct: 93 ACDPRNVYPAEIFSQPPGAHDADGRASMNLYGCSAQVDYAGSDVDVRRFLSVLQGRYDEN 152
Query: 129 TPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFL 188
TPP+RRL +D SNIIIY AGH A+ +FKFQD+EF+SS+DI++TL MM +QRRY ++VFL
Sbjct: 153 TPPTRRLLSDNTSNIIIYVAGHGAKSYFKFQDTEFLSSSDISETLTMMHQQRRYGRVVFL 212
Query: 189 VDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVLKDSKC 248
DTC A++LC ++APNVVCLA+S+A +SYS D G +++ W E +L + C
Sbjct: 213 ADTCHAIALCEHVEAPNVVCLAASDAESESYSCQYDEQLGTHMVSFWMNEMYLLLNGTSC 272
Query: 249 ------RPENGEVTLLQKSFYDFNYGPERLSLPQPLSEPAHFDAVNRPNAIREWKMDEFF 302
R + V++L +S+Y+FNY P R+ + S+PAH DAVN P A+REW + +F
Sbjct: 273 SNPLTRRIGDDAVSVLHQSWYNFNYHPYRVEASRNRSKPAHRDAVNDPTALREWIVADFV 332
Query: 303 CEQDRDKIPVELRYDL 318
C Q +PV++RYDL
Sbjct: 333 CGQVSAAVPVDVRYDL 348
>ref|NP_563825.1| (NM_100749) putative GPI-anchor transamidase (U32517) [Arabidopsis
thaliana]
Length = 388
Score = 418 bits (1066), Expect = e-116
Identities = 98/281 (34%), Positives = 163/281 (57%), Gaps = 12/281 (4%)
Query: 5 LLWLVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDD 64
+L LV L + + TN WAV++ +SR++FN RH N L++Y ++ G+ D+
Sbjct: 3 ILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFWFNYRHMANTLSLYRTVKRLGIPDE 62
Query: 65 HILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGR 124
I++ L+D AC+ R PA ++ + + NLYG N+ VDY Y+V V FL VL GR
Sbjct: 63 RIILMLADDMACNARNEYPAQVFN--NENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGR 120
Query: 125 YDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRK 184
++ P S+RL +DE S+I++Y GH ++F KFQD+E + S D+AD + M E+RR+++
Sbjct: 121 HENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKE 180
Query: 185 LVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVLK 244
L+ +VDTC+A +L ++++P V+ + SS +SYSHHLD G +V+ R+T+ L +
Sbjct: 181 LMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFE 240
Query: 245 ------DSKCR---PENGEVTLLQKSFYDFN-YGPERLSLP 275
++ L+ ++Y + Y P + +P
Sbjct: 241 RLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVP 281
>pir||T12043 probable legumain (EC 3.4.22.34) precursor - kidney bean
emb|CAB17078.1| (Z99956) asparagine-specific endopeptidase precursor [Phaseolus
vulgaris]
Length = 484
Score = 410 bits (1045), Expect = e-113
Identities = 58/264 (21%), Positives = 104/264 (38%), Gaps = 17/264 (6%)
Query: 8 LVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHIL 67
LV + P+ G WA++ + S ++N RH + Y L RK G+ D++I+
Sbjct: 28 LVGDFLRLPSDSGNGDNVHGTRWAILFAGSSGYWNYRHQADICHAYQLLRKGGLKDENII 87
Query: 68 VFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDE 127
VF+ D A + P I +P +Y + DY DV F L G +
Sbjct: 88 VFMYDDIAFNSENPRRGVIINSPN---GDEVYK-GVPKDYTGEDVTAHNFYAALLGDKSK 143
Query: 128 NTPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLV 186
T S + +++ N +I I+ + H ++ ++D+ + L Y+ LV
Sbjct: 144 LTGGSGKVVNSGPNDHIFIFYSDHGGPGVLGSPAGPYIYASDLNEVLKKKHASGTYKNLV 203
Query: 187 FLVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFE 238
F ++ C + S+ + NV +S A S+ + PP T +
Sbjct: 204 FYLEACESGSIFEGLLPEDINVYATTASNADESSWGTYCPGEDPSPPPEYSTCLGD--LY 261
Query: 239 FLEVLKDSKCRPENGEVTLLQKSF 262
+ ++DS N L + +
Sbjct: 262 SVAWMEDS--DRHNLRTETLHQQY 283
>emb|CAC18099.1| (AJ251453) putative legumain [Zea mays]
Length = 486
Score = 409 bits (1041), Expect = e-113
Identities = 60/258 (23%), Positives = 105/258 (40%), Gaps = 17/258 (6%)
Query: 14 LAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDS 73
A AA+ + WAV+++ S ++N RH + Y + +K G+ D++I+VF+ D
Sbjct: 35 RAAAADETDDGDVGTRWAVLIAGSSGYYNYRHQADICHAYQIMKKGGLKDENIIVFMYDD 94
Query: 74 YACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS- 132
A P P P I P + ++Y + DY DV V F VL G S
Sbjct: 95 IAHSPENPRPGVIINHP---QGGDVYA-GVPKDYTGRDVNVDNFFAVLLGNKTALRGGSG 150
Query: 133 RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTC 192
+ +D+ N +I ++ + H ++ D+ D L Y+ LVF ++ C
Sbjct: 151 KVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEAC 210
Query: 193 RALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFLEVLK 244
+ S+ + NV +S A S+ + PP T + + ++
Sbjct: 211 ESGSIFEGLLPNDINVYATTASNADESSWGTYCPGEFPSPPPEYDTCLGD--LYSVAWME 268
Query: 245 DSKCRPENGEVTLLQKSF 262
DS N L++ +
Sbjct: 269 DS--DFHNLRTESLKQQY 284
>emb|CAB64544.1| (AJ131718) legumain-like protease [Zea mays]
Length = 486
Score = 408 bits (1038), Expect = e-113
Identities = 59/258 (22%), Positives = 104/258 (39%), Gaps = 17/258 (6%)
Query: 14 LAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDS 73
A AA+ WAV+++ S ++N RH + Y + +K G+ D++I+VF+ D
Sbjct: 35 RAAAADETDDGAVGTRWAVLIAGSSGYYNYRHQADICHAYQIMKKGGLKDENIIVFMYDD 94
Query: 74 YACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS- 132
A P P P I P + ++Y + DY DV V F VL G S
Sbjct: 95 IAHSPENPRPGVIINHP---QGGDVYA-GVPKDYTGRDVNVDNFFAVLLGNKTALRGGSG 150
Query: 133 RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTC 192
+ +D+ + +I ++ + H ++ D+ D L Y+ LVF ++ C
Sbjct: 151 KVVDSGPDDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEAC 210
Query: 193 RALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFLEVLK 244
+ S+ + NV +S A S+ + PP T + + ++
Sbjct: 211 ESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLGD--LYSVAWME 268
Query: 245 DSKCRPENGEVTLLQKSF 262
DS N L++ +
Sbjct: 269 DS--DFHNLRTESLKQQY 284
>dbj|BAA76744.1| (D89971) asparaginyl endopeptidase (VmPE-1) [Vigna mungo]
Length = 483
Score = 408 bits (1038), Expect = e-112
Identities = 59/261 (22%), Positives = 106/261 (40%), Gaps = 20/261 (7%)
Query: 11 NLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFL 70
F APA + WAV+++ S ++N RH ++ Y L K G+ +++I+VF+
Sbjct: 33 RFFQAPATDENDE---GTRWAVLIAGSNGYWNYRHQSDVCHAYQLLTKGGLKEENIVVFM 89
Query: 71 SDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTP 130
D A + P P I +P ++Y + DY DV V F + G T
Sbjct: 90 YDDIAFNEENPRPGVIINSP---HGNDVYK-GVPKDYVGEDVTVNNFFAAILGNKSALTG 145
Query: 131 PS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLV 189
S + +++ N +I IY + H S +M ++D+ + L Y+ L F +
Sbjct: 146 GSGKVVNSGPNDHIFIYYSDHGGPGVLGMPTSPYMYASDLIEVLKKKHASGTYKSLAFYL 205
Query: 190 DTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFLE 241
+ C + S+ + N+ ++ A S+ + PP T + +
Sbjct: 206 EGCESGSIFGGLLPEGLNIYATTAANAEESSWGTYCPGDNPSPPPEYETCLGD--LYSVA 263
Query: 242 VLKDSKCRPENGEVTLLQKSF 262
++DS N L + F
Sbjct: 264 WMEDS--DIHNLRTETLHQQF 282
>gb|AAK15049.1|AF238384_1 (AF238384) asparaginyl endopeptidase [Vigna radiata]
Length = 483
Score = 407 bits (1037), Expect = e-112
Identities = 56/264 (21%), Positives = 103/264 (38%), Gaps = 17/264 (6%)
Query: 8 LVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHIL 67
LV + P+ G K WA++ + S ++N RH + Y + RK G+ +++I+
Sbjct: 28 LVGDFLRLPSDSGNDDNVKGTRWAILFAGSNGYWNYRHQADICHAYQILRKGGLKEENII 87
Query: 68 VFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDE 127
VF+ D A + P P I P ++Y + DY D F L G
Sbjct: 88 VFMYDDIAFNWDNPRPGVIINKPD---GDDVY-EGVPKDYTGEDATAHNFYSALLGDKSA 143
Query: 128 NTPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLV 186
T S + +++ + I I+ + H ++ ++D+ + L Y+ LV
Sbjct: 144 LTGGSGKVVNSGPDDRIFIFYSDHGGPGVLGTPAGPYIYASDLVEVLKKKHASGTYKNLV 203
Query: 187 FLVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFE 238
F ++ C A S+ + N+ +S A S+ + PP T +
Sbjct: 204 FYLEACEAGSIFEGLLPEDINIYATTASNAEESSWGTYCPGEYPSPPPEYSTCLGD--LY 261
Query: 239 FLEVLKDSKCRPENGEVTLLQKSF 262
+ ++DS N L + +
Sbjct: 262 SVAWMEDS--DRHNLRTESLHQQY 283
>emb|CAB64545.1| (AJ131719) legumain-like protease [Zea mays]
Length = 481
Score = 406 bits (1034), Expect = e-112
Identities = 58/259 (22%), Positives = 102/259 (38%), Gaps = 17/259 (6%)
Query: 14 LAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDS 73
P+ WAV+++ S ++N RH + Y + +K G+ D++I+VF+ D
Sbjct: 30 RLPSDRAAADDAVGTRWAVLIAGSNGYYNYRHQADICHAYQIMKKGGLKDENIVVFMYDD 89
Query: 74 YACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS- 132
A P P P I P + ++Y + DY DV V F VL G S
Sbjct: 90 IAHSPENPRPGVIINHP---QGGDVYA-GVPKDYTGRDVNVDNFFAVLLGNKTALRGGSG 145
Query: 133 RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTC 192
+ +D+ N +I ++ + H ++ D+ D L Y+ LVF ++ C
Sbjct: 146 KVVDSGPNDHISVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEAC 205
Query: 193 RALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFLEVLK 244
+ S+ + NV +S A S+ + PP T + + ++
Sbjct: 206 ESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLGD--LYSVAWME 263
Query: 245 DSKCRPENGEVTLLQKSFY 263
DS N L++ +
Sbjct: 264 DS--DFHNLRTESLKQQYN 280
>sp|P49043|VPE_CITSI VACUOLAR PROCESSING ENZYME PRECURSOR (VPE)
pir||S51117 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - sweet
orange
emb|CAA87720.1| (Z47793) cystein proteinase (by similarity) [Citrus sinensis]
prf||2208463A vascular processing protease [Citrus sinensis]
Length = 494
Score = 406 bits (1033), Expect = e-112
Identities = 61/255 (23%), Positives = 104/255 (39%), Gaps = 17/255 (6%)
Query: 17 AAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDSYAC 76
A + WAV+L+ S F+N RH + Y L RK G+ D++I+VF+ D A
Sbjct: 48 AKVNDDDDSVGTRWAVLLAGSNGFWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAF 107
Query: 77 DPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS-RRL 135
+ P P I P ++Y + DY DV V +F V+ G T S + +
Sbjct: 108 NEENPRPGVIINHP---HGDDVYK-GVPKDYTGEDVTVEKFFAVVLGNKTALTGGSGKVV 163
Query: 136 DTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTCRAL 195
D+ N +I I+ + H S ++ + ++ D L Y+ LVF ++ C +
Sbjct: 164 DSGPNDHIFIFYSDHGGPGVLGMPTSRYIYADELIDVLKKKHASGNYKSLVFYLEACESG 223
Query: 196 SLCLEIK--APNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFLEVLKDSK 247
S+ + N+ +S A S+ + PP T + + ++DS
Sbjct: 224 SIFEGLLLEGLNIYATTASNAEESSWGTYCPGEIPGPPPEYSTCLGD--LYSIAWMEDS- 280
Query: 248 CRPENGEVTLLQKSF 262
N L + +
Sbjct: 281 -DIHNLRTETLHQQY 294
>sp|P49045|VPE_SOYBN VACUOLAR PROCESSING ENZYME PRECURSOR (VPE)
pir||T07132 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - soybean
dbj|BAA06030.1| (D28876) cysteine proteinase [Glycine max]
Length = 495
Score = 405 bits (1032), Expect = e-112
Identities = 54/263 (20%), Positives = 101/263 (37%), Gaps = 19/263 (7%)
Query: 9 VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
V L P WAV+++ S + N RH + Y L K G+ +++I+V
Sbjct: 42 VIKLPTEPVDADSDE--VGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGLKEENIVV 99
Query: 69 FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
F+ D A + P I P E +LY + DY +V V+ G +
Sbjct: 100 FMYDDIATNELNPRHGVIINHP---EGEDLYA-GVPKDYTGDNVTTENLFAVILGDKSKL 155
Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
S + +++ I IY + H + ++ + D D L Y+++V
Sbjct: 156 KGGSGKVINSKPEDRIFIYYSDHGGPGILGMPNMPYLYAMDFIDVLKKKHASGSYKEMVI 215
Query: 188 LVDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
V+ C + S+ I N+ +S A +S+ + PP T +
Sbjct: 216 YVEACESGSVFEGIMPKDLNIYVTTASNAQENSWGTYCPGMDPSPPPEYITCLGD--LYS 273
Query: 240 LEVLKDSKCRPENGEVTLLQKSF 262
+ ++DS+ N + +++ +
Sbjct: 274 VAWMEDSEAH--NLKRESVKQQY 294
>sp|P49046|LEGU_CANEN Legumain precursor (Asparaginyl endopeptidase)
pir||JX0344 legumain (EC 3.4.22.34) precursor - jack bean
dbj|BAA06596.1| (D31787) asparaginyl endopeptidase (Legumain) [Canavalia
ensiformis]
Length = 475
Score = 405 bits (1031), Expect = e-112
Identities = 54/263 (20%), Positives = 105/263 (39%), Gaps = 22/263 (8%)
Query: 9 VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
V L P + WAV+++ S + N RH + Y L K G+ +++I+V
Sbjct: 25 VIQLPTEPVDDE-----VGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVV 79
Query: 69 FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
F+ D A + P P I P + P++Y + DY DV V+ G +
Sbjct: 80 FMYDDIAYNAMNPRPGVIINHP---QGPDVYA-GVPKDYTGEDVTPENLYAVILGDKSKV 135
Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
S + ++++ I I+ + H ++ F+ + D D L Y+++V
Sbjct: 136 KGGSGKVINSNPEDRIFIFYSDHGGPGVLGMPNAPFVYAMDFIDVLKKKHASGGYKEMVI 195
Query: 188 LVDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
++ C + S+ I N+ +S A +S+ + P T +
Sbjct: 196 YIEACESGSIFEGIMPKDLNIYVTTASNAQENSFGTYCPGMNPPPPEEYVTCLGD--LYS 253
Query: 240 LEVLKDSKCRPENGEVTLLQKSF 262
+ ++DS+ N + +Q+ +
Sbjct: 254 VSWMEDSETH--NLKRETVQQQY 274
>emb|CAB16318.1| (Z99174) cysteine proteinase precursor [Vicia narbonensis]
Length = 488
Score = 404 bits (1030), Expect = e-112
Identities = 58/263 (22%), Positives = 104/263 (39%), Gaps = 18/263 (6%)
Query: 9 VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
V L P + WAV+++ S + N RH + Y L K G+ +++I+V
Sbjct: 34 VIRLPGEPV-DADVEDEMGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVV 92
Query: 69 FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
F+ D A + P P I P + PN+Y + DY V F V+ G +
Sbjct: 93 FMYDDIAYNEMNPRPGVIINHP---QGPNVY-DGVPKDYNGDFVTAENFYAVILGDKSKV 148
Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
S + +++ I IY + H + ++ + D D L Y+K+V
Sbjct: 149 RGGSGKVINSKAEDRIFIYCSDHGGPGVLGMPNMPYVYAMDFIDVLKKKHASGGYKKMVI 208
Query: 188 LVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
V+ C + S+ I NV +S A +S+ + PP T +
Sbjct: 209 YVEACESGSIFEGIMPKDINVYVTTASNAQENSWGTYCPGVDPAPPPEYITCLGD--LYS 266
Query: 240 LEVLKDSKCRPENGEVTLLQKSF 262
+ ++DS+ N + +++ +
Sbjct: 267 VAWMEDSETH--NLKRETVKQQY 287
>gb|AAF69014.1|AF260827_1 (AF260827) cysteine protease [Ipomoea batatas]
Length = 492
Score = 404 bits (1028), Expect = e-111
Identities = 58/267 (21%), Positives = 104/267 (38%), Gaps = 21/267 (7%)
Query: 10 ANLFLAPAAEG----FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDH 65
A F PA E + WAV+++ S ++N RH + Y + + G+ D++
Sbjct: 32 ARRFFRPAEEENREADGDDSVGTRWAVLIAGSNGYWNYRHQADICHAYQILKAGGLKDEN 91
Query: 66 ILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRY 125
I+VF+ D A + P I +P ++Y + DY DV L V+ G
Sbjct: 92 IVVFMYDDIAYNEENPRKGIIINSP---HGEDVY-HGVPKDYTGDDVTANNLLAVILGDK 147
Query: 126 DENTPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRK 184
S + +D+ N +I IY + H S ++ + ++ L Y+
Sbjct: 148 SAVKGGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPYLYADELNAALKKKHAAGAYKS 207
Query: 185 LVFLVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWT 236
LVF ++ C + S+ I N+ +S A S+ + PP T +
Sbjct: 208 LVFYLEACESGSIFEGILPKDINIYATTASNAIESSWGTYCPGEYPSPPPEYETCLGD-- 265
Query: 237 FEFLEVLKDSKCRPENGEVTLLQKSFY 263
+ ++DS N L++ +
Sbjct: 266 LYSIAWMEDS--DIHNLRTESLKQQYN 290
>dbj|BAA76745.1| (D89972) asparaginyl endopeptidase (VmPE-1A) [Vigna mungo]
Length = 482
Score = 403 bits (1027), Expect = e-111
Identities = 54/263 (20%), Positives = 101/263 (37%), Gaps = 17/263 (6%)
Query: 9 VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
V + P+ G + WA++ + S ++N RH + Y + RK G+ +++I+V
Sbjct: 28 VGDFLRLPSDSGNDDNVQGTRWAILFAGSNGYWNYRHQADICHAYQILRKGGLKEENIIV 87
Query: 69 FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
F+ D A + P P I P ++Y + DY D F L G
Sbjct: 88 FMYDDIAFNWDNPRPGVIINKPD---GDDVY-EGVPKDYTGEDATAHNFYSALLGDKSAL 143
Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
T S + + + + I I+ + H ++ ++D+ + L Y+ LVF
Sbjct: 144 TGGSGKVVSSGPDDRIFIFYSDHGGPGVLGTPAGPYIYASDLVEVLKKKHASGTYKNLVF 203
Query: 188 LVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
++ C A S+ + N+ +S A S+ + PP T +
Sbjct: 204 YLEACEAGSIFEGLLPEDINIYATTASNAEESSWGTYCPGEYPSPPPEYSTCLGD--LYS 261
Query: 240 LEVLKDSKCRPENGEVTLLQKSF 262
+ ++DS N L + +
Sbjct: 262 VAWMEDS--DRHNLRTESLHQQY 282
>dbj|BAA84650.1| (AB025310) asparaginyl endopeptidase [Oryza sativa]
dbj|BAB85400.1| (AP003924) asparaginyl endopeptidase [Oryza sativa (japonica
cultivar-group)]
Length = 501
Score = 402 bits (1023), Expect = e-111
Identities = 58/256 (22%), Positives = 105/256 (40%), Gaps = 17/256 (6%)
Query: 17 AAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDSYAC 76
AA+ + WAV+++ S ++N RH + Y + ++ G+ D++I+VF+ D A
Sbjct: 53 AADDAAEAAEGTRWAVLIAGSNGYYNYRHQADVCHAYQIMKRGGLKDENIIVFMYDDIAH 112
Query: 77 DPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS-RRL 135
+P P P I P + ++Y + DY +V V+ VL G S + L
Sbjct: 113 NPENPRPGVIINHP---QGGDVYA-GVPKDYTGKEVNVKNLFAVLLGNKTAVKGGSGKVL 168
Query: 136 DTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTCRAL 195
D+ N +I I+ + H ++ D+ D L Y+ LVF ++ C +
Sbjct: 169 DSGPNDHIFIFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEACESG 228
Query: 196 SLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFLEVLKDSK 247
S+ + NV +S A S+ + PP T + + ++DS
Sbjct: 229 SIFEGLLPNGINVYATTASNADESSWGTYCPGEYPSPPPEYDTCLGD--LYSVAWMEDS- 285
Query: 248 CRPENGEVTLLQKSFY 263
N L++ +
Sbjct: 286 -DVHNLRTESLKQQYN 300
>sp|P49042|VPE_RICCO Vacuolar processing enzyme precursor (VPE)
pir||JQ2387 vacuolar processing enzyme (EC 3.4.22.-) precursor - castor bean
dbj|BAA04225.1| (D17401) precursor of vacuolar processing enzyme [Ricinus communis]
Length = 497
Score = 401 bits (1022), Expect = e-111
Identities = 56/264 (21%), Positives = 101/264 (38%), Gaps = 22/264 (8%)
Query: 21 FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDSYACDPRK 80
WAV+++ S F N RH + Y L RK G+ +++I+VF+ D A +
Sbjct: 54 DDDDQLGTRWAVLVAGSMGFGNYRHQADVCHAYQLLRKGGLKEENIIVFMYDDIAKNELN 113
Query: 81 PNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS-RRLDTDE 139
P P I P + ++Y + DY V + VL G S + +D+
Sbjct: 114 PRPGVIINHP---QGEDVYA-GVPKDYTGEHVTAKNLYAVLLGDKSAVQGGSGKVVDSKP 169
Query: 140 NSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTCRALSLCL 199
N I +Y + H + ++ + D + L Y+K+V V+ C + S+
Sbjct: 170 NDRIFLYYSDHGGPGVLGMPNLPYLYAMDFIEVLKKKHAAGGYKKMVIYVEACESGSIFE 229
Query: 200 EIKAPNV--VCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFLEVLKDSKCRPE 251
I +V +S A S+ + PP T + + ++DS+
Sbjct: 230 GIMPKDVDIYVTTASNAQESSWGTYCPGMEPSPPPEFTTCLGD--LYSVAWMEDSESH-- 285
Query: 252 NGEVTLLQKSFYD-----FNYGPE 270
N + +++ + NY
Sbjct: 286 NLKKETVKQQYSSVKARTSNYNTY 309
>gb|AAD04883.1| (AF082347) C13 endopeptidase NP1 precursor [Zea mays]
Length = 485
Score = 401 bits (1021), Expect = e-111
Identities = 60/264 (22%), Positives = 104/264 (38%), Gaps = 18/264 (6%)
Query: 10 ANLFLA-PAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
L AA+ WAV+++ S ++N RH + Y + +K G+ D++I+V
Sbjct: 29 IRLPSERAAADETDDDAVGTRWAVLIAGSNGYYNYRHQADICHAYQIMKKGGLKDENIVV 88
Query: 69 FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
F+ D A P P P I P + ++Y + DY DV V F VL G
Sbjct: 89 FMYDDIAHSPENPRPGVIINHP---QGGDVYA-GVPKDYTGRDVNVDNFFAVLLGNKTAL 144
Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
S + +D+ N +I ++ + H ++ D+ D L Y+ LVF
Sbjct: 145 RGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVF 204
Query: 188 LVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
++ C + S+ + NV +S A S+ + PP T +
Sbjct: 205 YLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLGD--LYS 262
Query: 240 LEVLKDSKCRPENGEVTLLQKSFY 263
+ ++DS N L++ +
Sbjct: 263 VAWMEDS--DFHNLRTESLKQQYN 284
>pir||T12044 probable legumain (EC 3.4.22.34) precursor - kidney bean
emb|CAB17079.1| (Z99957) legumain-like proteinase precursor [Phaseolus vulgaris]
Length = 493
Score = 401 bits (1020), Expect = e-110
Identities = 54/263 (20%), Positives = 101/263 (37%), Gaps = 19/263 (7%)
Query: 9 VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
V L WAV+++ S + N RH + Y L K G+ +++I+V
Sbjct: 40 VIKLPTQEVDAESDE--VGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVV 97
Query: 69 FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
F+ D A P P I P + P++Y + DY V F VL G +
Sbjct: 98 FMYDDIATHELNPRPGVIINNP---QGPDVYA-GVPKDYTGESVTSHNFFAVLLGDKSKV 153
Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
S + +++ I +Y + H + ++ + D D L Y+++V
Sbjct: 154 KGGSGKVINSKPEDRIFVYYSDHGGPGVLGMPNMPYLYAMDFIDVLKKKHASGGYKEMVI 213
Query: 188 LVDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHLD------PPSGFTVITRWTFEF 239
V+ C + S+ I N+ +S A +S+ + PP T +
Sbjct: 214 YVEACESGSIFEGIMPKDLNIYVTTASNAQENSWGTYCPGMYPPPPPEYITCLGD--LYS 271
Query: 240 LEVLKDSKCRPENGEVTLLQKSF 262
+ ++DS+ N + +++ +
Sbjct: 272 VAWMEDSESH--NLKKESVEQQY 292
>pir||S60050 vacuolar processing enzyme (EC 3.4.22.-) isozyme beta precursor -
Arabidopsis thaliana
dbj|BAA09615.1| (D61394) beta-VPE [Arabidopsis thaliana]
Length = 484
Score = 400 bits (1018), Expect = e-110
Identities = 56/274 (20%), Positives = 103/274 (37%), Gaps = 21/274 (7%)
Query: 10 ANLFLAPAAEGF-HGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
+ A WAV+++ S + N RH + Y + RK G+ +++I+V
Sbjct: 31 ILMPTEEANPADQDEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVV 90
Query: 69 FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
+ D A P P P T+ P ++Y + DY V F VL G
Sbjct: 91 LMYDDIANHPLNPRPGTLINHPD---GDDVYA-GVPKDYTGSSVTAANFYAVLLGDQKAV 146
Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
S + + + N +I +Y A H ++ + + D +TL Y+++V
Sbjct: 147 KGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVI 206
Query: 188 LVDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
V+ C + S+ I N+ +S A SY + P T +
Sbjct: 207 YVEACESGSIFEGIMPKDLNIYVTTASNAQESSYGTYCPGMNPSPPSEYITCLGD--LYS 264
Query: 240 LEVLKDSKCRPENGEVTLLQKSFY---DFNYGPE 270
+ ++DS+ N + +++ ++ NY
Sbjct: 265 VAWMEDSETH--NLKKETIKQQYHTMRTSNYNTY 296
>emb|CAC18100.1| (AJ251454) putative legumain [Zea mays]
Length = 485
Score = 400 bits (1018), Expect = e-110
Identities = 59/264 (22%), Positives = 104/264 (39%), Gaps = 18/264 (6%)
Query: 10 ANLFLA-PAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
L AA+ WAV+++ S ++N RH + Y + +K G+ D++I+V
Sbjct: 29 IRLPSERAAADETDDDAVGTRWAVLIAGSNGYYNYRHQADICHAYQIMKKGGLKDENIVV 88
Query: 69 FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
F+ D A P P P I P + ++Y + DY +V V F VL G
Sbjct: 89 FMYDDIAHSPENPRPGVIINHP---QGGDVYA-GVPKDYTGREVNVDNFFAVLLGNKTAL 144
Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
S + +D+ N +I ++ + H ++ D+ D L Y+ LVF
Sbjct: 145 RGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVF 204
Query: 188 LVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
++ C + S+ + NV +S A S+ + PP T +
Sbjct: 205 YLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLGD--LYS 262
Query: 240 LEVLKDSKCRPENGEVTLLQKSFY 263
+ ++DS N L++ +
Sbjct: 263 VAWMEDS--DFHNLRTESLKQQYN 284
>emb|CAC43295.1| (AJ309173) putative vacuolar processing enzyme [Beta vulgaris]
Length = 486
Score = 399 bits (1016), Expect = e-110
Identities = 58/269 (21%), Positives = 110/269 (40%), Gaps = 21/269 (7%)
Query: 7 WLVANLFLAPAAE----GFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMD 62
+V NL P+ + WAV+++ S ++N RH + Y + +K G+
Sbjct: 25 LMVDNLIRWPSDHPSIFESDDDSVGTRWAVLIAGSSGYWNYRHQADVCHAYQVLKKGGLK 84
Query: 63 DDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQ 122
D++I+VF+ D A D P P + +P ++Y + DY DV V F +
Sbjct: 85 DENIIVFMYDDIAYDEENPRPGVLINSP---YGHDVYA-GVPKDYTGEDVTVNNFFAAIL 140
Query: 123 GRYDENTPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRR 181
G D T S + +++ N +I I+ + H ++ + ++ +TL
Sbjct: 141 GNKDAITGGSGKVVNSGPNDHIFIFYSDHGGAGVLGMPTYPYLYADELIETLKEKHASGT 200
Query: 182 YRKLVFLVDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHLD------PPSGFTVIT 233
Y+ LV ++ C + S+ I N+ +S A S+ + PP T +
Sbjct: 201 YKSLVVYIEACESGSIFEGILPEGLNIYATTASNAVESSWGTYCPGQDPNVPPEYDTCLG 260
Query: 234 RWTFEFLEVLKDSKCRPENGEVTLLQKSF 262
+ ++DS+ N L++ +
Sbjct: 261 D--LYSVSWIEDSE--RHNLHTESLKQQY 285
>ref|NP_195020.1| (NM_119448) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
pir||T05302 vacuolar processing enzyme (EC 3.4.22.-) isozyme gamma precursor -
Arabidopsis thaliana
emb|CAA21203.1| (AL031804) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
emb|CAB80011.1| (AL161582) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
gb|AAK43975.1|AF370160_1 (AF370160) putative gamma-VPE protein [Arabidopsis thaliana]
gb|AAL11612.1|AF424619_1 (AF424619) AT4g32940/F26P21_60 [Arabidopsis thaliana]
gb|AAL15210.1| (AY059104) putative gamma-VPE protein [Arabidopsis thaliana]
Length = 494
Score = 399 bits (1016), Expect = e-110
Identities = 63/262 (24%), Positives = 109/262 (41%), Gaps = 18/262 (6%)
Query: 10 ANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVF 69
A+ F PA E N WAV+++ S ++N RH + Y L RK G+ +++I+VF
Sbjct: 41 ASRFFRPA-ENDDDSNSGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVF 99
Query: 70 LSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENT 129
+ D A + P P TI +P ++Y + DY DV V V+ G
Sbjct: 100 MYDDIANNYENPRPGTIINSP---HGKDVYQ-GVPKDYTGDDVNVDNLFAVILGDKTAVK 155
Query: 130 PPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFL 188
S + +D+ N +I I+ + H S ++ + D+ D L Y+ LVF
Sbjct: 156 GGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFY 215
Query: 189 VDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFL 240
++ C + S+ + N+ +S A S+ + PP T + +
Sbjct: 216 LEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEEPSPPPEYETCLGD--LYSV 273
Query: 241 EVLKDSKCRPENGEVTLLQKSF 262
++DS N + L + +
Sbjct: 274 AWMEDSG--MHNLQTETLHQQY 293
>ref|NP_176458.1| (NM_104948) beta-VPE [Arabidopsis thaliana]
gb|AAK56243.1|AF367254_1 (AF367254) At1g62710/F23N19_8 [Arabidopsis thaliana]
gb|AAL15381.1| (AY059156) At1g62710/F23N19_8 [Arabidopsis thaliana]
Length = 486
Score = 399 bits (1015), Expect = e-110
Identities = 56/276 (20%), Positives = 103/276 (37%), Gaps = 23/276 (8%)
Query: 10 ANLFLAPAAEGF-HGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
+ A WAV+++ S + N RH + Y + RK G+ +++I+V
Sbjct: 31 ILMPTEEANPADQDEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVV 90
Query: 69 FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
+ D A P P P T+ P ++Y + DY V F VL G
Sbjct: 91 LMYDDIANHPLNPRPGTLINHPD---GDDVYA-GVPKDYTGSSVTAANFYAVLLGDQKAV 146
Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
S + + + N +I +Y A H ++ + + D +TL Y+++V
Sbjct: 147 KGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVI 206
Query: 188 LVDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEF 239
V+ C + S+ I N+ +S A SY + P T +
Sbjct: 207 YVEACESGSIFEGIMPKDLNIYVTTASNAQESSYGTYCPGMNPSPPSEYITCLGD--LYS 264
Query: 240 LEVLKDSKCRPENGEVTLLQKSFY-----DFNYGPE 270
+ ++DS+ N + +++ ++ NY
Sbjct: 265 VAWMEDSETH--NLKKETIKQQYHTVKMRTSNYNTY 298
>sp|Q39119|VPEG_ARATH VACUOLAR PROCESSING ENZYME, GAMMA-ISOZYME PRECURSOR (GAMMA-VPE)
dbj|BAA18924.1| (D61395) gamma-VPE [Arabidopsis thaliana]
Length = 490
Score = 399 bits (1015), Expect = e-110
Identities = 63/262 (24%), Positives = 109/262 (41%), Gaps = 18/262 (6%)
Query: 10 ANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVF 69
A+ F PA E N WAV+++ S ++N RH + Y L RK G+ +++I+VF
Sbjct: 37 ASRFFRPA-ENDDDSNSGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVF 95
Query: 70 LSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENT 129
+ D A + P P TI +P ++Y + DY DV V V+ G
Sbjct: 96 MYDDIANNYENPRPGTIINSP---HGKDVYQ-GVPKDYTGDDVNVDNLFAVILGDKTAVK 151
Query: 130 PPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFL 188
S + +D+ N +I I+ + H S ++ + D+ D L Y+ LVF
Sbjct: 152 GGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFY 211
Query: 189 VDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFL 240
++ C + S+ + N+ +S A S+ + PP T + +
Sbjct: 212 LEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEEPSPPPEYETCLGD--LYSV 269
Query: 241 EVLKDSKCRPENGEVTLLQKSF 262
++DS N + L + +
Sbjct: 270 AWMEDSG--MHNLQTETLHQQY 289
>sp|P49044|VPE_VICSA VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) (PROTEINASE B)
pir||S49175 legumain (EC 3.4.22.34) precursor [similarity] - spring vetch
pir||S68984 cysteine proteinase precursor - spring vetch
emb|CAA84383.1| (Z34899) cysteine proteinase [Vicia sativa]
Length = 493
Score = 398 bits (1013), Expect = e-110
Identities = 56/273 (20%), Positives = 104/273 (37%), Gaps = 24/273 (8%)
Query: 11 NLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFL 70
F P + WA++L+ S ++N RH ++ Y L RK G +++I+VF+
Sbjct: 41 RFFREPKNDDDFE---GTRWAILLAGSNGYWNYRHQSDVCHAYQLLRKGGSKEENIIVFM 97
Query: 71 SDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTP 130
D A + P P I P ++Y + DY +V F L G T
Sbjct: 98 YDDIASNEENPRPGVIINKPD---GDDVYA-GVPKDYTGAEVHADNFYAALLGNKSALTG 153
Query: 131 PS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLV 189
S + +D+ N +I +Y H ++ ++D+ + L Y+ LVF +
Sbjct: 154 GSGKVVDSGPNDHIFVYYTDHGGPGVLGMPVGPYLYASDLNEVLKKKHASGTYKSLVFYL 213
Query: 190 DTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHLDPP------SGFTVITRWTFEFLE 241
+ C + S+ + N+ +S A S+ ++ T + +
Sbjct: 214 EACESGSIFEGLLPDDLNIYATTASNAEESSWGYYCPGDKPPPPPEYSTCLGD--LYSIA 271
Query: 242 VLKDSKCRPENGEVTLLQKSF----YDFNYGPE 270
++DS+ N + LQ+ + P
Sbjct: 272 WMEDSE--VHNLQTESLQQQYKLVKNRTISEPY 302
>ref|NP_180165.1| (NM_128154) putative vacuolar processing enzyme [Arabidopsis
thaliana]
sp|P49047|VPEA_ARATH VACUOLAR PROCESSING ENZYME, ALPHA-ISOZYME PRECURSOR (ALPHA-VPE)
pir||T02629 vacuolar processing enzyme (EC 3.4.22.-) isozyme alpha precursor -
Arabidopsis thaliana
gb|AAC31241.1| (AC004747) vacuolar processing enzyme, alpha-isozyme precursor
(alpha-VPE) [Arabidopsis thaliana]
dbj|BAA09614.2| (D61393) alpha-VPE [Arabidopsis thaliana]
gb|AAL24163.1| (AY058055) At2g25940/F17H15.3 [Arabidopsis thaliana]
Length = 478
Score = 397 bits (1012), Expect = e-110
Identities = 58/262 (22%), Positives = 107/262 (40%), Gaps = 21/262 (8%)
Query: 10 ANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVF 69
A+ F P + WAV+++ S ++N RH + Y L +K G+ +++I+VF
Sbjct: 29 ASKFFRPTENDDD----STKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVF 84
Query: 70 LSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENT 129
+ D A + P P I +P ++Y + DY +V V L V+ G
Sbjct: 85 MYDDIAKNEENPRPGVIINSPN---GEDVY-NGVPKDYTGDEVNVDNLLAVILGNKTALK 140
Query: 130 PPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFL 188
S + +D+ N +I IY + H S + + D+ D L + Y+ LVF
Sbjct: 141 GGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFY 200
Query: 189 VDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFL 240
++ C + S+ + N+ +S A S+ + P T + +
Sbjct: 201 LEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGD--LYSV 258
Query: 241 EVLKDSKCRPENGEVTLLQKSF 262
++DS+ N + L + +
Sbjct: 259 AWIEDSEKH--NLQTETLHEQY 278
>ref|XP_039644.1| (XM_039644) phosphatidylinositol glycan, class K [Homo sapiens]
gb|AAB81597.1| (AF022913) GPI transamidase [Homo sapiens]
gb|AAH20737.1|AAH20737 (BC020737) Unknown (protein for MGC:22559) [Homo sapiens]
Length = 395
Score = 397 bits (1011), Expect = e-109
Identities = 102/267 (38%), Positives = 158/267 (58%), Gaps = 11/267 (4%)
Query: 9 VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
VA + AE F TN WAV++ +SR++FN RH N L++Y ++ G+ D HI++
Sbjct: 25 VAASHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVL 84
Query: 69 FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
L+D AC+PR P PAT++ + + N+YG ++ VDY SY+V V FL VL GR +
Sbjct: 85 MLADDMACNPRNPKPATVFSH--KNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIPPS 142
Query: 129 TPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFL 188
TP S+RL +D+ SNI+IY GH F KFQDSE +++ ++AD MW++RRY +L+F+
Sbjct: 143 TPRSKRLLSDDRSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFI 202
Query: 189 VDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVLKD--- 245
+DTC+ S+ +PN++ LASS+ DS SH DP G ++ R+TF LE L++
Sbjct: 203 IDTCQGASMYERFYSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINP 262
Query: 246 -SKCRPENG-----EVTLLQKSFYDFN 266
S+ + + + + +
Sbjct: 263 ASQTNMNDLFQVCPKSLCVSTPGHRTD 289
>emb|CAA68871.1| (Y07596) gpi8 [Homo sapiens]
Length = 396
Score = 397 bits (1010), Expect = e-109
Identities = 102/267 (38%), Positives = 158/267 (58%), Gaps = 11/267 (4%)
Query: 9 VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
VA + AE F TN WAV++ +SR++FN RH N L++Y ++ G+ D HI++
Sbjct: 26 VAASHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVL 85
Query: 69 FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
L+D AC+PR P PAT++ + + N+YG ++ VDY SY+V V FL VL GR +
Sbjct: 86 MLADDMACNPRNPKPATVFSH--KNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIPPS 143
Query: 129 TPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFL 188
TP S+RL +D+ SNI+IY GH F KFQDSE +++ ++AD MW++RRY +L+F+
Sbjct: 144 TPRSKRLLSDDRSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFI 203
Query: 189 VDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVLKD--- 245
+DTC+ S+ +PN++ LASS+ DS SH DP G ++ R+TF LE L++
Sbjct: 204 IDTCQGASMYERFYSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINP 263
Query: 246 -SKCRPENG-----EVTLLQKSFYDFN 266
S+ + + + + +
Sbjct: 264 ASQTNMNDLFQVCPKSLCVSTPGHRTD 290
>gb|AAF89646.1|AF169019_1 (AF169019) seed maturation protein PM40 [Glycine max]
Length = 496
Score = 397 bits (1010), Expect = e-109
Identities = 53/264 (20%), Positives = 104/264 (39%), Gaps = 19/264 (7%)
Query: 9 VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
V L P WAV+++ S + N RH + Y L K G+ +++I+V
Sbjct: 41 VIKLPAEPVDADSDHE-VGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGLKEENIVV 99
Query: 69 FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
F+ D A D P P I P E ++Y + DY +V + V+ G ++
Sbjct: 100 FMYDDIATDELNPRPGVIINHP---EGQDVYA-GVPKDYTGENVTAQNLFAVILGDKNKV 155
Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
S + +++ I IY + H + ++ + D + L Y+K+V
Sbjct: 156 KGGSGKVINSKPEDRIFIYYSDHGGPGVLGMPNMPYLYAMDFIEVLKKKHASGGYKKMVI 215
Query: 188 LVDTCRALS-LCLEIKAPN--VVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFE 238
V+ C + + + I + + +S A +S+ + PP T +
Sbjct: 216 YVEACESGNHVLKGIMPKDLQIYVTTASNAQENSWGTYCPGMDPSPPPEYITCLGD--LY 273
Query: 239 FLEVLKDSKCRPENGEVTLLQKSF 262
+ ++DS+ N + +++ +
Sbjct: 274 SVAWMEDSETH--NLKRESVKQQY 295
>pir||T10944 cysteine proteinase (EC 3.4.22.-) precursor - spring vetch
emb|CAA07639.1| (AJ007743) cysteine proteinase precursor [Vicia sativa]
Length = 503
Score = 394 bits (1002), Expect = e-108
Identities = 59/264 (22%), Positives = 103/264 (38%), Gaps = 19/264 (7%)
Query: 9 VANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILV 68
V L P + WAV+++ S + N RH + Y L K G+ +++I+V
Sbjct: 48 VIRLPGEPV-DADVEDEIGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVV 106
Query: 69 FLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDEN 128
F+ D A P P I P + PN+Y + DY V V+ G +
Sbjct: 107 FMYDDIAYSEFNPRPGVIINHP---QGPNVY-DGVPKDYTGDFVTADNLYAVILGDKSKV 162
Query: 129 TPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVF 187
S + +++ I IY + H + ++ + D D L R Y+++V
Sbjct: 163 RGGSGKVINSKAEDRIFIYYSDHGGPGVLGMPNMPYVYAMDFIDVLKKKHASRGYQQMVI 222
Query: 188 LVDTCRALSLCLEIKAP--NVVCLASSEAHLDSYSHHL-------DPPSGFTVITRWTFE 238
V+ C + S+ I +V +S A S+ +L PP T +
Sbjct: 223 YVEACESGSVFQGIMPKDIDVYVTTASNAEESSWGTYLVPGVYPASPPEYITCLGD--LY 280
Query: 239 FLEVLKDSKCRPENGEVTLLQKSF 262
+ ++DS+ N + L++ F
Sbjct: 281 SVAWMEDSETH--NLKRETLKQQF 302
>gb|AAF89679.1| (AF169973) asparaginyl endopeptidase [Sesamum indicum]
Length = 489
Score = 393 bits (1001), Expect = e-108
Identities = 51/258 (19%), Positives = 98/258 (37%), Gaps = 18/258 (6%)
Query: 14 LAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDS 73
P N WAV+++ S F N RH + Y + +K G+ D++I+VF+ D
Sbjct: 40 RWPLDRRETEDNA-TRWAVLVAGSNGFGNYRHQADVCHAYQILKKGGLRDENIIVFMYDD 98
Query: 74 YACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS- 132
A + P I P ++Y + DY V V+ G S
Sbjct: 99 IAMNELNPRKGVIINHPT---GGDVYA-GVPKDYTGEQVTAENLYAVILGDKSAIKGGSG 154
Query: 133 RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTC 192
+ +D+ N I IY + H + ++ + D + L Y+++V V+ C
Sbjct: 155 KVVDSKPNDRIFIYYSDHGGPGVLGMPNMPYLYANDFIEVLKKKHASGTYKEMVIYVEAC 214
Query: 193 RALSLCLEIKA--PNVVCLASSEAHLDSYSHHLD------PPSGFTVITRWTFEFLEVLK 244
+ S+ + ++ +S A S+ + PP T + + ++
Sbjct: 215 ESGSVFEGLMPDDLDIYVTTASNAEESSWGTYCPGMDPPPPPEYITCLGD--LYSVAWME 272
Query: 245 DSKCRPENGEVTLLQKSF 262
DS+ N + +++ +
Sbjct: 273 DSESH--NLKRETVEQQY 288
>pir||S60049 vacuolar processing enzyme (EC 3.4.22.-) isozyme alpha precursor -
Arabidopsis thaliana
Length = 478
Score = 392 bits (998), Expect = e-108
Identities = 56/262 (21%), Positives = 105/262 (39%), Gaps = 21/262 (8%)
Query: 10 ANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVF 69
A+ F P + WAV+++ S ++N RH + Y +K G+ +++I+V
Sbjct: 29 ASKFFRPTENDDD----STKWAVLVAGSSGYWNYRHQADVCHAYQRLKKGGVKEENIVVL 84
Query: 70 LSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENT 129
+ D A + P P I +P ++Y + DY +V V L V+ G
Sbjct: 85 MYDDIAENEENPRPGVIINSPN---GEDVY-NGVPKDYTGDEVNVDNLLAVILGNKTALK 140
Query: 130 PPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFL 188
S + +D+ N +I IY + H S + + D+ D L + Y+ LVF
Sbjct: 141 GGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFY 200
Query: 189 VDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSHHL------DPPSGFTVITRWTFEFL 240
++ C + S+ + N+ +S A S+ + P T + +
Sbjct: 201 LEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGD--LYSV 258
Query: 241 EVLKDSKCRPENGEVTLLQKSF 262
++DS+ N + L + +
Sbjct: 259 AWIEDSEKH--NLQTETLHEQY 278
>gb|AAF99765.1|AC003981_15 (AC003981) F22O13.24 [Arabidopsis thaliana]
Length = 407
Score = 389 bits (990), Expect = e-107
Identities = 91/278 (32%), Positives = 154/278 (54%), Gaps = 23/278 (8%)
Query: 5 LLWLVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDD 64
+L LV L + + TN WAV++ +SR++ ++ G+ D+
Sbjct: 3 ILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFW--------------TVKRLGIPDE 48
Query: 65 HILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGR 124
I++ L+D AC+ R PA ++ + + NLYG N+ VDY Y+V V FL VL GR
Sbjct: 49 RIILMLADDMACNARNEYPAQVFN--NENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGR 106
Query: 125 YDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRK 184
++ P S+RL +DE S+I++Y GH ++F KFQD+E + S D+AD + M E+RR+++
Sbjct: 107 HENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKE 166
Query: 185 LVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVLK 244
L+ +VDTC+A +L ++++P V+ + SS +SYSHHLD G +V+ R+T+ L +
Sbjct: 167 LMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFE 226
Query: 245 ------DSKCRPENGEVTLLQKSFYDFN-YGPERLSLP 275
++ L+ ++Y + Y P + +P
Sbjct: 227 RLNIYDNASLNRSYDPRLLMSTAYYRTDLYQPHLVEVP 264
>pir||T00731 hypothetical protein F22O13.26 - Arabidopsis thaliana
Length = 428
Score = 386 bits (983), Expect = e-106
Identities = 93/285 (32%), Positives = 157/285 (54%), Gaps = 16/285 (5%)
Query: 5 LLWLVANLFLAPAAEGFHGMNKTNTWAVILSSSRY----FFNLRHTTNALAMYHLCRKHG 60
+L LV L + + TN WAV++ +SR+ L + L + ++ G
Sbjct: 3 ILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFCSLHSLVLTFIFSLLGVSRTVKRLG 62
Query: 61 MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120
+ D+ I++ L+D AC+ R PA ++ + + NLYG N+ VDY Y+V V FL V
Sbjct: 63 IPDERIILMLADDMACNARNEYPAQVFN--NENHKLNLYGDNVEVDYRGYEVTVENFLRV 120
Query: 121 LQGRYDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQR 180
L GR++ P S+RL +DE S+I++Y GH ++F KFQD+E + S D+AD + M E+R
Sbjct: 121 LTGRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKR 180
Query: 181 RYRKLVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFL 240
R+++L+ +VDTC+A +L ++++P V+ + SS +SYSHHLD G +V+ R+T+ L
Sbjct: 181 RFKELMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTL 240
Query: 241 EVLK------DSKCR---PENGEVTLLQKSFYDFN-YGPERLSLP 275
+ ++ L+ ++Y + Y P + +P
Sbjct: 241 AFFERLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVP 285
>dbj|BAB29018.1| (AK013853) data source:SPTR, source key:O14822,
evidence:ISS~homolog to GPI TRANSAMIDASE~putative [Mus
musculus]
Length = 395
Score = 381 bits (971), Expect = e-105
Identities = 96/263 (36%), Positives = 150/263 (56%), Gaps = 11/263 (4%)
Query: 13 FLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSD 72
+ AE F TN WAV++ +SR++FN RH N L++Y ++ G+ D HI++ L+D
Sbjct: 29 HIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVLMLAD 88
Query: 73 SYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS 132
AC+ R P PAT++ + + N+YG ++ VDY SY V FL VL +TP S
Sbjct: 89 DMACNARNPKPATVFSH--KNMELNVYGDDVEVDYRSYRGTVENFLRVLTXEVPPSTPRS 146
Query: 133 RRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTC 192
+RL +D+ SNI+IY GH F KFQDSE +++ ++AD MW++RRY +L+F++DTC
Sbjct: 147 KRLLSDDKSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTC 206
Query: 193 RALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVLKD----SKC 248
+ S+ +PN++ LASS+ DS SH DP G ++ R+TF LE K+ S+
Sbjct: 207 QGASMYERFYSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFWKEINPASQT 266
Query: 249 RPENG-----EVTLLQKSFYDFN 266
+ + + + +
Sbjct: 267 NMNDLFQVCPKSLCVSTPGHRTD 289
>gb|AAF73260.1|AF154349_1 (AF154349) legumain [Rattus norvegicus]
Length = 435
Score = 375 bits (955), Expect = e-103
Identities = 60/268 (22%), Positives = 113/268 (41%), Gaps = 19/268 (7%)
Query: 5 LLWLVANLFLAPAAEG------FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRK 58
++W VA L G + W VI++ S ++N RH +A Y + +
Sbjct: 1 MIWKVAVLLSLVLGAGAVHIGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHR 60
Query: 59 HGMDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFL 118
+G+ D+ I+V + D A + P P + P ++Y + DY DV FL
Sbjct: 61 NGIPDEQIIVMMYDDIANNEENPTPGVVINRPN---GTDVYK-GVPKDYTGEDVTPENFL 116
Query: 119 GVLQGRYDEN--TPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMM 176
VL+G + + L + ++ +Y H A F + E + D+ T+ M
Sbjct: 117 AVLRGDEEAVKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNKTIRYM 175
Query: 177 WEQRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRW 235
+E + Y+K+VF ++ C + S+ + +V ++ + SY+ + D T + W
Sbjct: 176 YEHKMYQKMVFYIEACESGSMMNHLPDDIDVYATTAANPNESSYACYYDEER-STYLGDW 234
Query: 236 TFEFLEVLKDSKCRPENGEVTLLQKSFY 263
+ ++DS E+ L K ++
Sbjct: 235 --YSVNWMEDS--DVEDLTKETLHKQYH 258
>ref|NP_071562.1| (NM_022226) protease, cysteine, 1 (legumain) [Rattus norvegicus]
dbj|BAA84750.1| (AB032766) legumain [Rattus norvegicus]
Length = 435
Score = 374 bits (952), Expect = e-102
Identities = 60/268 (22%), Positives = 112/268 (41%), Gaps = 19/268 (7%)
Query: 5 LLWLVANLFLAPAAEG------FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRK 58
+ W VA L G + W VI++ S ++N RH +A Y + +
Sbjct: 1 MTWRVAVLLSLVLGAGAVHIGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHR 60
Query: 59 HGMDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFL 118
+G+ D+ I+V + D A + P P + P ++Y + DY DV FL
Sbjct: 61 NGIPDEQIIVMMYDDIANNEENPTPGVVINRPN---GTDVYK-GVPKDYTGEDVTPENFL 116
Query: 119 GVLQGRYDEN--TPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMM 176
VL+G + + L + ++ +Y H A F + E + D+ T+ M
Sbjct: 117 AVLRGDEEAVKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNKTIRYM 175
Query: 177 WEQRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRW 235
+E + Y+K+VF ++ C + S+ + +V ++ + SY+ + D T + W
Sbjct: 176 YEHKMYQKMVFYIEACESGSMMNHLPDDIDVYATTAANPNESSYACYYDEER-STYLGDW 234
Query: 236 TFEFLEVLKDSKCRPENGEVTLLQKSFY 263
+ ++DS E+ L K ++
Sbjct: 235 --YSVNWMEDS--DVEDLTKETLHKQYH 258
>ref|NP_035305.1| (NM_011175) protease, cysteine, 1; preprolegumain [Mus musculus]
emb|CAA04439.1| (AJ000990) legumain [Mus musculus]
gb|AAF21659.1|AF044266_1 (AF044266) preprolegumain; cysteine Protease [Mus musculus]
Length = 435
Score = 373 bits (948), Expect = e-102
Identities = 62/268 (23%), Positives = 111/268 (41%), Gaps = 19/268 (7%)
Query: 5 LLWLVANLFLAPAAEG------FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRK 58
+ W VA L G + W VI++ S ++N RH +A Y + +
Sbjct: 1 MTWRVAVLLSLVLGAGAVPVGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHR 60
Query: 59 HGMDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFL 118
+G+ D+ I+V + D A P P + P ++Y + DY DV FL
Sbjct: 61 NGIPDEQIIVMMYDDIANSEENPTPGVVINRPN---GTDVYK-GVLKDYTGEDVTPENFL 116
Query: 119 GVLQGRYDEN--TPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMM 176
VL+G + + L + ++ IY H A F + + + D+ T+ M
Sbjct: 117 AVLRGDAEAVKGKGSGKVLKSGPRDHVFIYFTDHGATGILVFPN-DDLHVKDLNKTIRYM 175
Query: 177 WEQRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRW 235
+E + Y+K+VF ++ C + S+ + NV ++ SY+ + D G T + W
Sbjct: 176 YEHKMYQKMVFYIEACESGSMMNHLPDDINVYATTAANPKESSYACYYDEERG-TYLGDW 234
Query: 236 TFEFLEVLKDSKCRPENGEVTLLQKSFY 263
+ ++DS E+ L K ++
Sbjct: 235 --YSVNWMEDS--DVEDLTKETLHKQYH 258
>dbj|BAA09530.1| (D55696) cysteine protease [Homo sapiens]
Length = 433
Score = 370 bits (942), Expect = e-101
Identities = 61/266 (22%), Positives = 113/266 (41%), Gaps = 17/266 (6%)
Query: 5 LLWLVANLFLAPAAEG----FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG 60
++W VA G + WAVI++ S ++N RH +A Y + ++G
Sbjct: 1 MVWKVAVFLSVALGIGAVPIDDPEDGGKHWAVIVAGSNGWYNYRHQADACHAYQIIHRNG 60
Query: 61 MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120
+ D+ I+V + D A P P + P ++Y + DY DV + FL V
Sbjct: 61 IPDEQIVVMMYDDIAYSEDNPTPGIVINRPN---GTDVYQ-GVPKDYTGEDVTPQNFLAV 116
Query: 121 LQGRYDENT--PPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWE 178
L+G + + L + ++ IY H + F + E + D+ +T+ M++
Sbjct: 117 LRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYK 175
Query: 179 QRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRWTF 237
+ YRK+VF ++ C + S+ + NV ++ SY+ + D T + W
Sbjct: 176 HKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKR-STYLGDW-- 232
Query: 238 EFLEVLKDSKCRPENGEVTLLQKSFY 263
+ ++DS E+ L K ++
Sbjct: 233 YSVNWMEDS--DVEDLTKETLHKQYH 256
>ref|XP_007399.4| (XM_007399) legumain [Homo sapiens]
Length = 401
Score = 370 bits (941), Expect = e-101
Identities = 60/266 (22%), Positives = 112/266 (41%), Gaps = 17/266 (6%)
Query: 5 LLWLVANLFLAPAAEG----FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG 60
++W VA G + W VI++ S ++N RH +A Y + ++G
Sbjct: 1 MVWKVAVFLSVALGIGAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNG 60
Query: 61 MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120
+ D+ I+V + D A P P + P ++Y + DY DV + FL V
Sbjct: 61 IPDEQIVVMMYDDIAYSEDNPTPGIVINRPN---GTDVYQ-GVPKDYTGEDVTPQNFLAV 116
Query: 121 LQGRYDENT--PPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWE 178
L+G + + L + ++ IY H + F + E + D+ +T+ M++
Sbjct: 117 LRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYK 175
Query: 179 QRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRWTF 237
+ YRK+VF ++ C + S+ + NV ++ SY+ + D T + W
Sbjct: 176 HKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKR-STYLGDW-- 232
Query: 238 EFLEVLKDSKCRPENGEVTLLQKSFY 263
+ ++DS E+ L K ++
Sbjct: 233 YSVNWMEDS--DVEDLTKETLHKQYH 256
>gb|AAH03061.1|AAH03061 (BC003061) protease, cysteine, 1 (legumain) [Homo sapiens]
Length = 433
Score = 368 bits (937), Expect = e-101
Identities = 60/266 (22%), Positives = 112/266 (41%), Gaps = 17/266 (6%)
Query: 5 LLWLVANLFLAPAAEG----FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG 60
++W VA G + W VI++ S ++N RH +A Y + ++G
Sbjct: 1 MVWKVAVFLSVALGIGAIPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNG 60
Query: 61 MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120
+ D+ I+V + D A P P + P ++Y + DY DV + FL V
Sbjct: 61 IPDEQIVVMMYDDIAYSEDNPTPGIVINRPN---GTDVYQ-GVPKDYTGEDVTPQNFLAV 116
Query: 121 LQGRYDENT--PPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWE 178
L+G + + L + ++ IY H + F + E + D+ +T+ M++
Sbjct: 117 LRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYK 175
Query: 179 QRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRWTF 237
+ YRK+VF ++ C + S+ + NV ++ SY+ + D T + W
Sbjct: 176 HKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKR-STYLGDW-- 232
Query: 238 EFLEVLKDSKCRPENGEVTLLQKSFY 263
+ ++DS E+ L K ++
Sbjct: 233 YSVNWMEDS--DVEDLTKETLHKQYH 256
>emb|CAB51545.1| (AJ243876) vacuolar processing enzyme [Lycopersicon esculentum]
Length = 460
Score = 368 bits (937), Expect = e-101
Identities = 60/262 (22%), Positives = 110/262 (41%), Gaps = 24/262 (9%)
Query: 19 EGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDSYACDP 78
+ + + WAV+++ S+ ++N RH N Y L +K G+ D+HI+VF+ D A +P
Sbjct: 29 DEDYEDSIGTKWAVLVAGSKEWYNYRHQANLCHAYQLLKKGGLKDEHIIVFMYDDIANNP 88
Query: 79 RKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPS-RRLDT 137
P P I P ++Y + DY D + F V+ G T S + +++
Sbjct: 89 ENPRPGVIINNP---HGHDVYK-GVPKDYTGKDCNAQNFYSVILGNKSALTGGSGKVVNS 144
Query: 138 DENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTCRALSL 197
N I IY H A + + + D+ + L R Y+K+VF ++ C + S+
Sbjct: 145 GPNDYIFIYYTDHGAPGLVGMPEDPPVYAIDLNEVLKKKHASRTYKKMVFYLEACDSGSM 204
Query: 198 CLEIKA--PNVVCLASSEAHLDSYSHHL-----------DPPSGF--TVITRWTFEFLEV 242
++ N+ SS+ D ++ + PP F + +
Sbjct: 205 FADLLDEGLNIYATTSSKPDEDGWATYCYFTGDTSCYGECPPKDFKDNCLGD--LFSVSW 262
Query: 243 LKDSKCRPENGEVTLLQKSFYD 264
L++S + +V L+K +
Sbjct: 263 LENSDLH--DLQVETLEKQYLR 282
>ref|NP_005597.1| (NM_005606) legumain; protease, cysteine, 1 (legumain) [Homo
sapiens]
sp|Q99538|LEGU_HUMAN Legumain precursor (Asparaginyl endopeptidase)
emb|CAA70989.1| (Y09862) legumain [Homo sapiens]
gb|AAH08004.1|AAH08004 (BC008004) protease, cysteine, 1 (legumain) [Homo sapiens]
Length = 433
Score = 368 bits (936), Expect = e-101
Identities = 60/266 (22%), Positives = 112/266 (41%), Gaps = 17/266 (6%)
Query: 5 LLWLVANLFLAPAAEG----FHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG 60
++W VA G + W VI++ S ++N RH +A Y + ++G
Sbjct: 1 MVWKVAVFLSVALGIGAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNG 60
Query: 61 MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120
+ D+ I+V + D A P P + P ++Y + DY DV + FL V
Sbjct: 61 IPDEQIVVMMYDDIAYSEDNPTPGIVINRPN---GTDVYQ-GVPKDYTGEDVTPQNFLAV 116
Query: 121 LQGRYDENT--PPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWE 178
L+G + + L + ++ IY H + F + E + D+ +T+ M++
Sbjct: 117 LRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYK 175
Query: 179 QRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRWTF 237
+ YRK+VF ++ C + S+ + NV ++ SY+ + D T + W
Sbjct: 176 HKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKR-STYLGDW-- 232
Query: 238 EFLEVLKDSKCRPENGEVTLLQKSFY 263
+ ++DS E+ L K ++
Sbjct: 233 YSVNWMEDS--DVEDLTKETLHKQYH 256
>pir||T40853 probable cysteine proteinase (EC 3.4.22.-) [similarity] - fission
yeast (Schizosaccharomyces pombe)
emb|CAB57844.1| (AL121783) putative gpi-anchor transamidase [Schizosaccharomyces
pombe]
emb|CAC13970.1| (AJ250428) GPI8p transamidase [Schizosaccharomyces pombe]
Length = 380
Score = 367 bits (934), Expect = e-100
Identities = 107/269 (39%), Positives = 159/269 (58%), Gaps = 7/269 (2%)
Query: 4 MLLWLVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDD 63
M++ VA L L + TN WAV++S+SR++FN RHT N L +Y ++ G+ D
Sbjct: 1 MIVQFVALLLLNLLQIIAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPD 60
Query: 64 DHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQG 123
I++ ++D YAC+ R P T++ +A +LYG I +DY Y+V V F+ +L
Sbjct: 61 SQIILMIADDYACNSRNLFPGTVFDNADRA--LDLYGEEIEIDYKGYEVTVEAFIRLLTE 118
Query: 124 RYDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYR 183
R ENTP S+RL T+E SNI+IY GH + F KFQD+E +SS D+AD + + + +RY
Sbjct: 119 RVPENTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYN 178
Query: 184 KLVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVL 243
+++F+VDTC+A SL +I +PNV+ + SSE SYSHH D G VI R+TF LE L
Sbjct: 179 EILFMVDTCQANSLYTKIYSPNVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSNLEFL 238
Query: 244 KDSKCRPENGEVTLLQKSFYDFNYGPERL 272
++ R ++ +Q Y P +
Sbjct: 239 EN---RVDSKSKLTMQDLINS--YNPYEI 262
>dbj|BAB69947.1| (AB060129) legumain [Bos taurus]
Length = 433
Score = 365 bits (930), Expect = e-100
Identities = 59/265 (22%), Positives = 110/265 (41%), Gaps = 17/265 (6%)
Query: 5 LLWLVANLFLAPAAEGF----HGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHG 60
++W L G + W VI++ S ++N RH +A Y + ++G
Sbjct: 1 MIWEFTVLLSLVLGTGAVPLEDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIVHRNG 60
Query: 61 MDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGV 120
+ D+ I+V + D A P P + P ++Y + DY DV + FL V
Sbjct: 61 IPDEQIIVMMYDDIANSEDNPTPGIVINRPN---GSDVYQ-GVLKDYTGEDVTPKNFLAV 116
Query: 121 LQGRYDENT--PPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWE 178
L+G + + L + ++ +Y H A F + E + D+ +T+ M+E
Sbjct: 117 LRGDAEAVKGVGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNETIRYMYE 175
Query: 179 QRRYRKLVFLVDTCRALSLCLEIKAP-NVVCLASSEAHLDSYSHHLDPPSGFTVITRWTF 237
+ Y+K+VF ++ C + S+ + NV ++ SY+ + D T + W
Sbjct: 176 HKMYQKMVFYIEACESGSMMNHLPPDINVYATTAANPRESSYACYYDEQR-STFLGDW-- 232
Query: 238 EFLEVLKDSKCRPENGEVTLLQKSF 262
+ ++DS E+ L K +
Sbjct: 233 YSVNWMEDS--DVEDLTKETLHKQY 255
>gb|AAF45703.1| (AE003422) EG:133E12.3 gene product [Drosophila melanogaster]
Length = 322
Score = 363 bits (923), Expect = 3e-99
Identities = 91/226 (40%), Positives = 140/226 (61%), Gaps = 2/226 (0%)
Query: 19 EGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDSYACDP 78
+ TN WAV++ +SR++FN RH N L++Y ++ G+ D I++ ++D AC+
Sbjct: 32 DAAQRSTHTNNWAVLVDASRFWFNYRHVANVLSIYRSVKRLGIPDSQIILMIADDMACNA 91
Query: 79 RKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPSRRLDTD 138
R P P +Y + N+YG ++ VDY Y+V V F+ +L GR T S++L +D
Sbjct: 92 RNPRPGQVYNNAN--QHINVYGDDVEVDYRGYEVTVENFVRLLTGRTQNGTARSKKLLSD 149
Query: 139 ENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTCRALSLC 198
SN++IY GH + F KFQDSE ++S ++AD + MWE++RY +L F+VDTC+A SL
Sbjct: 150 AGSNVLIYLTGHGGDGFLKFQDSEEITSQELADGIQQMWEKKRYNELFFMVDTCQAASLY 209
Query: 199 LEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVLK 244
+ +PNV+ +ASS DS SHH+DP G +I R+T+ LE L+
Sbjct: 210 EKFTSPNVLAVASSLVGEDSLSHHVDPSIGVYMIDRYTYYALEFLE 255
>ref|NP_188656.1| (NM_112912) vacuolar processing enzyme/asparaginyl endopeptidase,
putative [Arabidopsis thaliana]
dbj|BAB01880.1| (AP000383) vacuolar processing enzyme (proteinase) [Arabidopsis
thaliana]
Length = 466
Score = 358 bits (910), Expect = 9e-98
Identities = 58/254 (22%), Positives = 104/254 (40%), Gaps = 17/254 (6%)
Query: 19 EGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDSYACDP 78
E K WAV+++ S ++N RH + Y + RK G+ D++I+VF+ D A
Sbjct: 36 ESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAYQILRKGGLKDENIIVFMYDDIAFSS 95
Query: 79 RKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPP-SRRLDT 137
P P I P ++Y + DY V V+ F VL G T + + +
Sbjct: 96 ENPRPGVIINKPD---GEDVYK-GVPKDYTKEAVNVQNFYNVLLGNESGVTGGNGKVVKS 151
Query: 138 DENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTCRALSL 197
N NI IY A H A + + + D + L M ++++Y K+V V+ C + S+
Sbjct: 152 GPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMHKRKKYNKMVIYVEACESGSM 211
Query: 198 CLEIKA--PNVVCLASSEAHLDSYSHHLDPPSGF------TVITRWTFEFLEVLKDSKCR 249
I N+ + ++ + S+ + T + + L+DS
Sbjct: 212 FEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEIGTCLGD--TFSISWLEDSDLH 269
Query: 250 PENGEVTLLQKSFY 263
+ L++ ++
Sbjct: 270 --DMSKETLEQQYH 281
>ref|NP_010618.1| (NC_001136) Protein involved in the attachment of
glycosylphosphatidylinositol (GPI) anchors to proteins;
Gpi8p [Saccharomyces cerevisiae]
sp|P49018|GPI8_YEAST GPI-ANCHOR TRANSAMIDASE
pir||S59796 probable membrane protein YDR331w - yeast (Saccharomyces
cerevisiae)
gb|AAB64766.1| (U32517) Ydr331wp [Saccharomyces cerevisiae]
Length = 411
Score = 357 bits (909), Expect = 1e-97
Identities = 104/270 (38%), Positives = 158/270 (58%), Gaps = 10/270 (3%)
Query: 2 LPMLLWLVANLFLAPA--AEGFHGMNK--TNTWAVILSSSRYFFNLRHTTNALAMYHLCR 57
LP+LL + L L+ A + H + TN WAV++S+SR++FN RH N L+MY +
Sbjct: 7 LPLLLLYIFLLPLSGANNTDAAHEVIATNTNNWAVLVSTSRFWFNYRHMANVLSMYRTVK 66
Query: 58 KHGMDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRF 117
+ G+ D I++ LSD AC+ R P +++ + +LYG ++ VDY Y+V V F
Sbjct: 67 RLGIPDSQIILMLSDDVACNSRNLFPGSVFN--NKDHAIDLYGDSVEVDYRGYEVTVENF 124
Query: 118 LGVLQGRYDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMW 177
+ +L R+ E+ P S+RL TDENSNI IY GH + F KFQD+E ++S DIAD M+
Sbjct: 125 IRLLTDRWTEDHPKSKRLLTDENSNIFIYMTGHGGDDFLKFQDAEEIASEDIADAFQQMY 184
Query: 178 EQRRYRKLVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTF 237
E++RY ++ F++DTC+A ++ + +PN++ + SSE SYSHH D G VI R+T+
Sbjct: 185 EKKRYNEIFFMIDTCQANTMYSKFYSPNILAVGSSEMDESSYSHHSDVEIGVAVIDRFTY 244
Query: 238 EFLEVLKDSKCRPENGEVTLLQKSFYDFNY 267
L+ L+ LQ F F +
Sbjct: 245 YCLDFLEQIDKN----STLTLQDLFDSFTF 270
>emb|CAB42651.1| (AJ238881) putative preprolegumain [Nicotiana tabacum]
Length = 419
Score = 353 bits (898), Expect = 2e-96
Identities = 43/227 (18%), Positives = 86/227 (36%), Gaps = 17/227 (7%)
Query: 45 HTTNALAMYHLCRKHGMDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIR 104
H + Y + ++ G+ D++I+VF+ D A P P I P ++Y +
Sbjct: 1 HQADVCHAYQILKRGGLKDENIVVFMYDDIAKSELNPRPGVIINHPN---GSDVYA-GVP 56
Query: 105 VDYASYDVGVRRFLGVLQGRYDENTPPS-RRLDTDENSNIIIYAAGHSAEKFFKFQDSEF 163
DY V VL G S + +D+ N I +Y + H + F
Sbjct: 57 KDYTGEHVTAANLYAVLLGDKSAVKGGSGKIVDSKPNDRIFLYYSDHGGPGVLGMPNMPF 116
Query: 164 MSSTDIADTLMMMWEQRRYRKLVFLVDTCRALSLCLEIKA--PNVVCLASSEAHLDSYSH 221
+ + D + L Y+++V ++ C + S+ + N+ +S A S+
Sbjct: 117 LYAKDFIEVLKKKHAAGTYKEMVLYIEACESGSVFEGMMPEDLNIYVTTASNAEESSWGT 176
Query: 222 HLD------PPSGFTVITRWTFEFLEVLKDSKCRPENGEVTLLQKSF 262
+ PP T + + ++DS+ N + +++ +
Sbjct: 177 YCPGMDPPPPPEYITCLGD--LYSVAWMEDSESH--NLKKETIKQQY 219
CPU time: 92.70 user secs. 2.28 sys. secs 94.98 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.314 0.179 0.583
Gapped
Lambda K H
0.270 0.0629 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 255118333
Number of Sequences: 887402
Number of extensions: 14913996
Number of successful extensions: 27949
Number of sequences better than 10.0: 77
Number of HSP's better than 10.0 without gapping: 71
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 27541
Number of HSP's gapped (non-prelim): 83
length of query: 319
length of database: 277,845,442
effective HSP length: 52
effective length of query: 267
effective length of database: 231,700,538
effective search space: 61864043646
effective search space used: 61864043646
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.0 bits)
S2: 74 (32.8 bits)