Sequences with E-value BETTER than threshold (0.002) 542 e-153
541 e-153
526 e-148
526 e-148
523 e-147
522 e-147
522 e-147
519 e-146
519 e-146
515 e-145
513 e-144
513 e-144
513 e-144
513 e-144
513 e-144
511 e-144
511 e-144
510 e-143
510 e-143
509 e-143
508 e-143
507 e-142
505 e-142
505 e-142
505 e-142
505 e-142
505 e-142
505 e-142
505 e-142
504 e-141
503 e-141
500 e-140
497 e-139
496 e-139
494 e-138
489 e-137
487 e-136
487 e-136
485 e-136
485 e-136
485 e-136
484 e-135
478 e-134
466 e-130
452 e-126
449 e-125
445 e-124
445 e-124
445 e-124
439 e-122
Alignments
>ref|XP_039644.1| (XM_039644) phosphatidylinositol glycan, class K [Homo sapiens]
gb|AAB81597.1| (AF022913) GPI transamidase [Homo sapiens]
gb|AAH20737.1|AAH20737 (BC020737) Unknown (protein for MGC:22559) [Homo sapiens]
Length = 395
Score = 542 bits (1382), Expect = e-153
Identities = 179/386 (46%), Positives = 245/386 (63%), Gaps = 17/386 (4%)
Query: 5 FVALLLLNLLQIIAA----------ESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVK 54
+LLL+ + A+ S HTNNWAVL+ TSRFWFNYRH AN L +YRSVK
Sbjct: 14 LATVLLLSFGSVAASHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYRSVK 73
Query: 55 RLGIPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIR 114
RLGIPDS I+LM+ADD ACN RN P TVF + + L++YG+++E+DY+ YEVTVE F+R
Sbjct: 74 RLGIPDSHIVLMLADDMACNPRNPKPATVFSHKNMELNVYGDDVEVDYRSYEVTVENFLR 133
Query: 115 LLTERVPENTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQH 174
+LT R+P +TP SKRLL+++RSNILIYMTGHGG+GF+KFQD+EE+++ +LADA EQ+ Q
Sbjct: 134 VLTGRIPPSTPRSKRLLSDDRSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMWQK 193
Query: 175 KRYNEILFMVDTCQANSLYTKIYSPNVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSN 234
+RYNE+LF++DTCQ S+Y + YSPN++A+ SS+VG S SH D IGV ++DR+TF
Sbjct: 194 RRYNELLFIIDTCQGASMYERFYSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYV 253
Query: 235 LEFLENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIEL 294
LEFLE ++ S+ M DL STPG + +R P ++LITDFFG+VR +E+
Sbjct: 254 LEFLE-EINPASQTNMNDLFQVCPKSLCVSTPGHRTDLFQRDPKNVLITDFFGSVRKVEI 312
Query: 295 HSEKINWMLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNLKSFLPPTRELKYKKHP 354
+E I E S K Q + + K + + +LK HP
Sbjct: 313 TTETIKLQQDSEIME-SSYKE----DQMDEKLMEPLKYAEQLPVAQIIHQKPKLK-DWHP 366
Query: 355 ISRIISAVVCISFSIGFPYYASKYLK 380
I + + + F Y K++K
Sbjct: 367 PGGFILGLWALIIMVFFKTYGIKHMK 392
>emb|CAA68871.1| (Y07596) gpi8 [Homo sapiens]
Length = 396
Score = 541 bits (1381), Expect = e-153
Identities = 179/386 (46%), Positives = 245/386 (63%), Gaps = 17/386 (4%)
Query: 5 FVALLLLNLLQIIAA----------ESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVK 54
+LLL+ + A+ S HTNNWAVL+ TSRFWFNYRH AN L +YRSVK
Sbjct: 15 LATVLLLSFGSVAASHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYRSVK 74
Query: 55 RLGIPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIR 114
RLGIPDS I+LM+ADD ACN RN P TVF + + L++YG+++E+DY+ YEVTVE F+R
Sbjct: 75 RLGIPDSHIVLMLADDMACNPRNPKPATVFSHKNMELNVYGDDVEVDYRSYEVTVENFLR 134
Query: 115 LLTERVPENTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQH 174
+LT R+P +TP SKRLL+++RSNILIYMTGHGG+GF+KFQD+EE+++ +LADA EQ+ Q
Sbjct: 135 VLTGRIPPSTPRSKRLLSDDRSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMWQK 194
Query: 175 KRYNEILFMVDTCQANSLYTKIYSPNVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSN 234
+RYNE+LF++DTCQ S+Y + YSPN++A+ SS+VG S SH D IGV ++DR+TF
Sbjct: 195 RRYNELLFIIDTCQGASMYERFYSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYV 254
Query: 235 LEFLENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIEL 294
LEFLE ++ S+ M DL STPG + +R P ++LITDFFG+VR +E+
Sbjct: 255 LEFLE-EINPASQTNMNDLFQVCPKSLCVSTPGHRTDLFQRDPKNVLITDFFGSVRKVEI 313
Query: 295 HSEKINWMLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNLKSFLPPTRELKYKKHP 354
+E I E S K Q + + K + + +LK HP
Sbjct: 314 TTETIKLQQDSEIME-SSYKE----DQMDEKLMEPLKYAEQLPVAQIIHQKPKLK-DWHP 367
Query: 355 ISRIISAVVCISFSIGFPYYASKYLK 380
I + + + F Y K++K
Sbjct: 368 PGGFILGLWALIIMVFFKTYGIKHMK 393
>pir||T40853 probable cysteine proteinase (EC 3.4.22.-) [similarity] - fission
yeast (Schizosaccharomyces pombe)
emb|CAB57844.1| (AL121783) putative gpi-anchor transamidase [Schizosaccharomyces
pombe]
emb|CAC13970.1| (AJ250428) GPI8p transamidase [Schizosaccharomyces pombe]
Length = 380
Score = 526 bits (1342), Expect = e-148
Identities = 380/380 (100%), Positives = 380/380 (100%)
Query: 1 MIVQFVALLLLNLLQIIAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPD 60
MIVQFVALLLLNLLQIIAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPD
Sbjct: 1 MIVQFVALLLLNLLQIIAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPD 60
Query: 61 SQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERV 120
SQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERV
Sbjct: 61 SQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERV 120
Query: 121 PENTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEI 180
PENTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEI
Sbjct: 121 PENTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEI 180
Query: 181 LFMVDTCQANSLYTKIYSPNVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSNLEFLEN 240
LFMVDTCQANSLYTKIYSPNVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSNLEFLEN
Sbjct: 181 LFMVDTCQANSLYTKIYSPNVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSNLEFLEN 240
Query: 241 RVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIELHSEKIN 300
RVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIELHSEKIN
Sbjct: 241 RVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIELHSEKIN 300
Query: 301 WMLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNLKSFLPPTRELKYKKHPISRIIS 360
WMLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNLKSFLPPTRELKYKKHPISRIIS
Sbjct: 301 WMLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNLKSFLPPTRELKYKKHPISRIIS 360
Query: 361 AVVCISFSIGFPYYASKYLK 380
AVVCISFSIGFPYYASKYLK
Sbjct: 361 AVVCISFSIGFPYYASKYLK 380
>sp|P49043|VPE_CITSI VACUOLAR PROCESSING ENZYME PRECURSOR (VPE)
pir||S51117 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - sweet
orange
emb|CAA87720.1| (Z47793) cystein proteinase (by similarity) [Citrus sinensis]
prf||2208463A vascular processing protease [Citrus sinensis]
Length = 494
Score = 526 bits (1341), Expect = e-148
Identities = 72/362 (19%), Positives = 141/362 (38%), Gaps = 40/362 (11%)
Query: 18 AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRN 77
+ S WAVL++ S ++NYRH A++ Y+ +++ G+ D II+ + DD A N N
Sbjct: 52 DDDDSVGTRWAVLLAGSNGFWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNEEN 111
Query: 78 LFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPEN-TPASKRLLTNERS 136
PG + N D+Y + + DY G +VTVE F ++ + K + +
Sbjct: 112 PRPGVII-NHPHGDDVY-KGVPKDYTGEDVTVEKFFAVVLGNKTALTGGSGKVVDSGPND 169
Query: 137 NILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKI 196
+I I+ + HGG G + + + +++L D +++ H Y ++F ++ C++ S++ +
Sbjct: 170 HIFIFYSDHGGPGVLGMPTSRYIYADELIDVLKKKHASGNYKSLVFYLEACESGSIFEGL 229
Query: 197 YSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSKSK 247
N+ A +S SS+ + + + D ++ + +E +
Sbjct: 230 LLEGLNIYATTASNAEESSWGTYCPGEIPGPPPEYSTCLGDLYSIAWMEDSDIH-----N 284
Query: 248 LTMQDLINSYNPYEIHSTPG-----VQPINLRRSPDDILITDFFGNVRDIELHSEKINWM 302
L + L Y + + + + G + N+
Sbjct: 285 LRTETLHQQYELVKTRTASYNSYGSHVMQYGDIGLSKNNLFTYLGT------NPANDNYT 338
Query: 303 LPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTRELKYKKH 353
EN+ +P+ K V Q D+ +G K F + H
Sbjct: 339 FVDENSLRPASK---AVNQRDADLLHFWDKYRKAPEGTPRKAEAQKQFFEAMSHRMHVDH 395
Query: 354 PI 355
I
Sbjct: 396 SI 397
>pir||T12043 probable legumain (EC 3.4.22.34) precursor - kidney bean
emb|CAB17078.1| (Z99956) asparagine-specific endopeptidase precursor [Phaseolus
vulgaris]
Length = 484
Score = 523 bits (1335), Expect = e-147
Identities = 70/377 (18%), Positives = 141/377 (36%), Gaps = 45/377 (11%)
Query: 9 LLLNLLQIIAA----ESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQII 64
L+ + L++ + ++ H WA+L + S ++NYRH A++ Y+ +++ G+ D II
Sbjct: 28 LVGDFLRLPSDSGNGDNVHGTRWAILFAGSSGYWNYRHQADICHAYQLLRKGGLKDENII 87
Query: 65 LMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPEN- 123
+ + DD A NS N G + N+ ++Y + + DY G +VT F L +
Sbjct: 88 VFMYDDIAFNSENPRRGVII-NSPNGDEVY-KGVPKDYTGEDVTAHNFYAALLGDKSKLT 145
Query: 124 TPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFM 183
+ K + + +I I+ + HGG G + + + DL + +++ H Y ++F
Sbjct: 146 GGSGKVVNSGPNDHIFIFYSDHGGPGVLGSPAGPYIYASDLNEVLKKKHASGTYKNLVFY 205
Query: 184 VDTCQANSLYTKIYSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSN 234
++ C++ S++ + NV A +S SS+ + + + D ++ +
Sbjct: 206 LEACESGSIFEGLLPEDINVYATTASNADESSWGTYCPGEDPSPPPEYSTCLGDLYSVAW 265
Query: 235 LEFLENRVDSKSKLTMQDLINSYNPYEIHSTP------GVQPINLRRSPDDILITDFFGN 288
+E + L + L Y + + ++ + G
Sbjct: 266 MEDSDRH-----NLRTETLHQQYKLVKERTISGGLYYGSHVMQYGDVGLSKDILFHYLGT 320
Query: 289 VRDIELHSEKINWMLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLK 339
N EN+ S K V Q D+ +G K
Sbjct: 321 D------PANENLTFVDENSLWSSSK---AVNQRDADLVHFWDKFRKAPEGSPKKNEARK 371
Query: 340 SFLPPTRELKYKKHPIS 356
L + +
Sbjct: 372 QVLEVMSHRMHIDDSVE 388
>gb|AAF69014.1|AF260827_1 (AF260827) cysteine protease [Ipomoea batatas]
Length = 492
Score = 522 bits (1332), Expect = e-147
Identities = 72/366 (19%), Positives = 138/366 (37%), Gaps = 39/366 (10%)
Query: 15 QIIAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACN 74
+ + S WAVLI+ S ++NYRH A++ Y+ +K G+ D I++ + DD A N
Sbjct: 44 READGDDSVGTRWAVLIAGSNGYWNYRHQADICHAYQILKAGGLKDENIVVFMYDDIAYN 103
Query: 75 SRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPE-NTPASKRLLTN 133
N G + N+ D+Y + DY G +VT + ++ + K + +
Sbjct: 104 EENPRKGIII-NSPHGEDVY-HGVPKDYTGDDVTANNLLAVILGDKSAVKGGSGKVVDSG 161
Query: 134 ERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLY 193
+I IY + HGG G + + L +++L A+++ H Y ++F ++ C++ S++
Sbjct: 162 PNDHIFIYYSDHGGPGVLGMPTSPYLYADELNAALKKKHAAGAYKSLVFYLEACESGSIF 221
Query: 194 TKIYSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDS 244
I N+ A +S SS+ + + + D ++ + +E +
Sbjct: 222 EGILPKDINIYATTASNAIESSWGTYCPGEYPSPPPEYETCLGDLYSIAWMEDSDIH--- 278
Query: 245 KSKLTMQDLINSYNPYEIHSTP------GVQPINLRRSPDDILITDFFGNVRDIELHSEK 298
L + L YN + + + + + G +
Sbjct: 279 --NLRTESLKQQYNLVKDRTLNGNTAYGSHVMQYGDLELNADSLFMYMGT------NPAN 330
Query: 299 INWMLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTRELK 349
N+ E + K S R V Q D+ +G K F
Sbjct: 331 ENFTFVDEKSLKLSAPRR-AVNQRDADLLHFWDKFRNAPEGSARKSEAQKQFTEAITHRT 389
Query: 350 YKKHPI 355
+ + I
Sbjct: 390 HLDNSI 395
>ref|NP_563825.1| (NM_100749) putative GPI-anchor transamidase (U32517) [Arabidopsis
thaliana]
Length = 388
Score = 522 bits (1331), Expect = e-147
Identities = 163/375 (43%), Positives = 237/375 (62%), Gaps = 9/375 (2%)
Query: 2 IVQFVALLLLNLLQIIAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDS 61
I+ V LL + + + HTNNWAVL+ TSRFWFNYRH AN L +YR+VKRLGIPD
Sbjct: 3 ILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFWFNYRHMANTLSLYRTVKRLGIPDE 62
Query: 62 QIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVP 121
+IILM+ADD ACN+RN +P VF+N + L+LYG+ +E+DY+GYEVTVE F+R+LT R
Sbjct: 63 RIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHE 122
Query: 122 ENTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEIL 181
P SKRLL++E S+IL+YMTGHGGD F+KFQDAEEL S DLADA++Q+ + +R+ E++
Sbjct: 123 NAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELM 182
Query: 182 FMVDTCQANSLYTKIYSPNVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSNLEFLENR 241
MVDTCQA +L+ ++ SP VLAIGSS G +SYSHH D DIGV+V+DRFT+ L F E R
Sbjct: 183 IMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFE-R 241
Query: 242 VDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIELHSEKINW 301
++ ++ L SY+P + ST + + ++ +T+FFG+V + +
Sbjct: 242 LNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHT-DSAYK 300
Query: 302 MLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNLKSFLP-------PTRELKYKKHP 354
+ + + F +++D++++ + I N + R ++K
Sbjct: 301 AFSSKISERKINSEMPFNQLSEHDLKEELENTNIPNDELIAEKQKCPYSQMRADLHEKVE 360
Query: 355 ISRIISAVVCISFSI 369
+ VV +S ++
Sbjct: 361 KLENVDTVVNLSIAV 375
>dbj|BAA76744.1| (D89971) asparaginyl endopeptidase (VmPE-1) [Vigna mungo]
Length = 483
Score = 519 bits (1325), Expect = e-146
Identities = 69/379 (18%), Positives = 140/379 (36%), Gaps = 45/379 (11%)
Query: 13 LLQIIA-AESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDY 71
Q A E+ WAVLI+ S ++NYRH ++V Y+ + + G+ + I++ + DD
Sbjct: 34 FFQAPATDENDEGTRWAVLIAGSNGYWNYRHQSDVCHAYQLLTKGGLKEENIVVFMYDDI 93
Query: 72 ACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPEN-TPASKRL 130
A N N PG + N+ D+Y + + DY G +VTV F + + K +
Sbjct: 94 AFNEENPRPGVII-NSPHGNDVY-KGVPKDYVGEDVTVNNFFAAILGNKSALTGGSGKVV 151
Query: 131 LTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQAN 190
+ +I IY + HGG G + + + + DL + +++ H Y + F ++ C++
Sbjct: 152 NSGPNDHIFIYYSDHGGPGVLGMPTSPYMYASDLIEVLKKKHASGTYKSLAFYLEGCESG 211
Query: 191 SLYTKIYSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENR 241
S++ + N+ A ++ SS+ + + + D ++ + +E +
Sbjct: 212 SIFGGLLPEGLNIYATTAANAEESSWGTYCPGDNPSPPPEYETCLGDLYSVAWMEDSDIH 271
Query: 242 VDSKSKLTMQDLINSYNPYEIHSTP------GVQPINLRRSPDDILITDFFGNVRDIELH 295
L + L + + + ++ + G +
Sbjct: 272 -----NLRTETLHQQFELVKQRTMNGNSAYGSHVMQYGDVGLSKNNVSLYLGT------N 320
Query: 296 SEKINWMLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTR 346
N+ +N+ P K V Q D+ G K L
Sbjct: 321 PANDNFPFREKNSLVPPSK---AVNQRDADLVHFWDKFPKAPLGSSRKSVAQKQILEAMS 377
Query: 347 ELKYKKHP---ISRIISAV 362
+ I +++ +
Sbjct: 378 HRMHIDDSVTLIGKLLFGI 396
>gb|AAK15049.1|AF238384_1 (AF238384) asparaginyl endopeptidase [Vigna radiata]
Length = 483
Score = 519 bits (1324), Expect = e-146
Identities = 68/370 (18%), Positives = 134/370 (35%), Gaps = 44/370 (11%)
Query: 9 LLLNLLQIIA----AESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQII 64
L+ + L++ + ++ WA+L + S ++NYRH A++ Y+ +++ G+ + II
Sbjct: 28 LVGDFLRLPSDSGNDDNVKGTRWAILFAGSNGYWNYRHQADICHAYQILRKGGLKEENII 87
Query: 65 LMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPEN- 123
+ + DD A N N PG + N D+Y E + DY G + T F L
Sbjct: 88 VFMYDDIAFNWDNPRPGVII-NKPDGDDVY-EGVPKDYTGEDATAHNFYSALLGDKSALT 145
Query: 124 TPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFM 183
+ K + + I I+ + HGG G + + + DL + +++ H Y ++F
Sbjct: 146 GGSGKVVNSGPDDRIFIFYSDHGGPGVLGTPAGPYIYASDLVEVLKKKHASGTYKNLVFY 205
Query: 184 VDTCQANSLYTKIYSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSN 234
++ C+A S++ + N+ A +S SS+ + + + D ++ +
Sbjct: 206 LEACEAGSIFEGLLPEDINIYATTASNAEESSWGTYCPGEYPSPPPEYSTCLGDLYSVAW 265
Query: 235 LEFLENRVDSKSKLTMQDLINSYNPYEIHSTP-----GVQPINLRRSPDDILITDFFGNV 289
+E + L + L Y + + + + G
Sbjct: 266 MEDSDRH-----NLRTESLHQQYKVVKDRTLSGGWYGSHVMQYGDVEFSKDTLFLYLGTD 320
Query: 290 RDIELHSEKINWMLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKS 340
N EN+ S + V Q D+ +G K
Sbjct: 321 ------PANDNLTFVDENSLWSSS---TAVNQRDADLVHFWHKFRKAPEGSPKKNEARKQ 371
Query: 341 FLPPTRELKY 350
L +
Sbjct: 372 VLEVMSHRMH 381
>dbj|BAA76745.1| (D89972) asparaginyl endopeptidase (VmPE-1A) [Vigna mungo]
Length = 482
Score = 515 bits (1313), Expect = e-145
Identities = 67/369 (18%), Positives = 133/369 (35%), Gaps = 44/369 (11%)
Query: 10 LLNLLQIIA----AESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIIL 65
+ + L++ + ++ WA+L + S ++NYRH A++ Y+ +++ G+ + II+
Sbjct: 28 VGDFLRLPSDSGNDDNVQGTRWAILFAGSNGYWNYRHQADICHAYQILRKGGLKEENIIV 87
Query: 66 MIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPEN-T 124
+ DD A N N PG + N D+Y E + DY G + T F L
Sbjct: 88 FMYDDIAFNWDNPRPGVII-NKPDGDDVY-EGVPKDYTGEDATAHNFYSALLGDKSALTG 145
Query: 125 PASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMV 184
+ K + + I I+ + HGG G + + + DL + +++ H Y ++F +
Sbjct: 146 GSGKVVSSGPDDRIFIFYSDHGGPGVLGTPAGPYIYASDLVEVLKKKHASGTYKNLVFYL 205
Query: 185 DTCQANSLYTKIYSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNL 235
+ C+A S++ + N+ A +S SS+ + + + D ++ + +
Sbjct: 206 EACEAGSIFEGLLPEDINIYATTASNAEESSWGTYCPGEYPSPPPEYSTCLGDLYSVAWM 265
Query: 236 EFLENRVDSKSKLTMQDLINSYNPYEIHSTP-----GVQPINLRRSPDDILITDFFGNVR 290
E + L + L Y + + + + G
Sbjct: 266 EDSDRH-----NLRTESLHQQYKVVKDRTLSGGWYGSHVMQYGDVEFSKDALFLYLGTD- 319
Query: 291 DIELHSEKINWMLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSF 341
N EN+ S + V Q D+ +G K
Sbjct: 320 -----PANDNLTFVDENSLWSSS---TAVNQRDADLVHFWHKFRKAPEGSPKKNEARKQV 371
Query: 342 LPPTRELKY 350
L +
Sbjct: 372 LEVMSHRMH 380
>dbj|BAA84650.1| (AB025310) asparaginyl endopeptidase [Oryza sativa]
dbj|BAB85400.1| (AP003924) asparaginyl endopeptidase [Oryza sativa (japonica
cultivar-group)]
Length = 501
Score = 513 bits (1309), Expect = e-144
Identities = 76/365 (20%), Positives = 139/365 (37%), Gaps = 42/365 (11%)
Query: 18 AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRN 77
AAE++ WAVLI+ S ++NYRH A+V Y+ +KR G+ D II+ + DD A N N
Sbjct: 57 AAEAAEGTRWAVLIAGSNGYYNYRHQADVCHAYQIMKRGGLKDENIIVFMYDDIAHNPEN 116
Query: 78 LFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPE-NTPASKRLLTNERS 136
PG + N + D+Y + DY G EV V+ +L + K L +
Sbjct: 117 PRPGVII-NHPQGGDVYA-GVPKDYTGKEVNVKNLFAVLLGNKTAVKGGSGKVLDSGPND 174
Query: 137 NILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKI 196
+I I+ + HGG G + L +DL D +++ H Y ++F ++ C++ S++ +
Sbjct: 175 HIFIFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEACESGSIFEGL 234
Query: 197 YSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSKSK 247
NV A +S SS+ + + + D ++ + +E +
Sbjct: 235 LPNGINVYATTASNADESSWGTYCPGEYPSPPPEYDTCLGDLYSVAWMEDSDVH-----N 289
Query: 248 LTMQDLINSYNPYEIHSTP-------GVQPINLRRSPDDILITDFFGNVRDIELHSEKIN 300
L + L YN + ++ + + + G+ + N
Sbjct: 290 LRTESLKQQYNLVKERTSVQHTYYSGSHVMEYGSLELNAHHVFMYMGS------NPANDN 343
Query: 301 WMLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTRELKYK 351
+N+ + V Q D+ + K L +
Sbjct: 344 ATFVEDNSLPSFSR---AVNQRDADLVYFWQKYRKLPESSPEKNEARKQLLEMMAHRSHV 400
Query: 352 KHPIS 356
+ +
Sbjct: 401 DNSVE 405
>ref|NP_180165.1| (NM_128154) putative vacuolar processing enzyme [Arabidopsis
thaliana]
sp|P49047|VPEA_ARATH VACUOLAR PROCESSING ENZYME, ALPHA-ISOZYME PRECURSOR (ALPHA-VPE)
pir||T02629 vacuolar processing enzyme (EC 3.4.22.-) isozyme alpha precursor -
Arabidopsis thaliana
gb|AAC31241.1| (AC004747) vacuolar processing enzyme, alpha-isozyme precursor
(alpha-VPE) [Arabidopsis thaliana]
dbj|BAA09614.2| (D61393) alpha-VPE [Arabidopsis thaliana]
gb|AAL24163.1| (AY058055) At2g25940/F17H15.3 [Arabidopsis thaliana]
Length = 478
Score = 513 bits (1309), Expect = e-144
Identities = 70/371 (18%), Positives = 138/371 (36%), Gaps = 42/371 (11%)
Query: 9 LLLNLLQIIAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIA 68
L + + WAVL++ S ++NYRH A+V Y+ +K+ G+ + I++ +
Sbjct: 28 LASKFFR-PTENDDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMY 86
Query: 69 DDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPE-NTPAS 127
DD A N N PG + N+ D+Y + DY G EV V+ + ++ +
Sbjct: 87 DDIAKNEENPRPGVII-NSPNGEDVY-NGVPKDYTGDEVNVDNLLAVILGNKTALKGGSG 144
Query: 128 KRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTC 187
K + + +I IY + HGG G + + L + DL D +++ + Y ++F ++ C
Sbjct: 145 KVVDSGPNDHIFIYYSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEAC 204
Query: 188 QANSLYTKIYSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFL 238
++ S++ + N+ A +S SS+ + + + D ++ + +E
Sbjct: 205 ESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDS 264
Query: 239 ENRVDSKSKLTMQDLINSYNPYEIHST------PGVQPINLRRSPDDILITDFFGNVRDI 292
E L + L Y + + + F G
Sbjct: 265 EKH-----NLQTETLHEQYELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGT---- 315
Query: 293 ELHSEKINWMLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLP 343
+ N+ EN+ +P + Q D+ +G + K L
Sbjct: 316 --NPADENFTFVNENSIRPPSR---VTNQRDADLVHFWHKYQKAPEGSARKVEAQKQVLE 370
Query: 344 PTRELKYKKHP 354
+ +
Sbjct: 371 AMSHRLHVDNS 381
>gb|AAF73260.1|AF154349_1 (AF154349) legumain [Rattus norvegicus]
Length = 435
Score = 513 bits (1309), Expect = e-144
Identities = 95/388 (24%), Positives = 162/388 (41%), Gaps = 28/388 (7%)
Query: 1 MIVQFVALLLLNLLQII-----AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKR 55
MI + LL L L +W V+++ S W+NYRH A+ Y+ + R
Sbjct: 1 MIWKVAVLLSLVLGAGAVHIGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHR 60
Query: 56 LGIPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRL 115
GIPD QII+M+ DD A N N PG V N D+Y + + DY G +VT E F+ +
Sbjct: 61 NGIPDEQIIVMMYDDIANNEENPTPGVVI-NRPNGTDVY-KGVPKDYTGEDVTPENFLAV 118
Query: 116 LTERVPE--NTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQ 173
L + K L + R ++ +Y T HG G + F + E+L +DL I +++
Sbjct: 119 LRGDEEAVKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNKTIRYMYE 177
Query: 174 HKRYNEILFMVDTCQANSLYTKIYSP-NVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTF 232
HK Y +++F ++ C++ S+ + +V A ++ SSY+ + D + + D ++
Sbjct: 178 HKMYQKMVFYIEACESGSMMNHLPDDIDVYATTAANPNESSYACYYDEERSTYLGDWYSV 237
Query: 233 SNLEFLENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDI 292
+ +E + LT + L Y+ + H+ +S + + F G
Sbjct: 238 NWMEDSDVED-----LTKETLHKQYHLVKSHTNTSHVMQYGNKSISTMKVMQFQGMKHR- 291
Query: 293 ELHSEKINWMLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNLKSFLP-PTRELKYK 351
S I+ PS V ++ L + L
Sbjct: 292 --ASSPISLPPVTHLDLTPS----PDVPLT--ILKRKLLRTNNMKESQVLVGQIQHLLDA 343
Query: 352 KHPISRIISAVVCISFSIGFPYYASKYL 379
+H I + + +V + GF A K+L
Sbjct: 344 RHIIEKSVQKIVSL--LAGFGETAQKHL 369
>ref|NP_195020.1| (NM_119448) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
pir||T05302 vacuolar processing enzyme (EC 3.4.22.-) isozyme gamma precursor -
Arabidopsis thaliana
emb|CAA21203.1| (AL031804) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
emb|CAB80011.1| (AL161582) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
gb|AAK43975.1|AF370160_1 (AF370160) putative gamma-VPE protein [Arabidopsis thaliana]
gb|AAL11612.1|AF424619_1 (AF424619) AT4g32940/F26P21_60 [Arabidopsis thaliana]
gb|AAL15210.1| (AY059104) putative gamma-VPE protein [Arabidopsis thaliana]
Length = 494
Score = 513 bits (1308), Expect = e-144
Identities = 68/373 (18%), Positives = 142/373 (37%), Gaps = 44/373 (11%)
Query: 18 AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRN 77
+S+ WAVL++ S ++NYRH A++ Y+ +++ G+ + I++ + DD A N N
Sbjct: 51 DDDSNSGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYEN 110
Query: 78 LFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPE-NTPASKRLLTNERS 136
PGT+ N+ D+Y + + DY G +V V+ ++ + K + +
Sbjct: 111 PRPGTII-NSPHGKDVY-QGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPND 168
Query: 137 NILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKI 196
+I I+ + HGG G + + L + DL D +++ H Y ++F ++ C++ S++ +
Sbjct: 169 HIFIFYSDHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGL 228
Query: 197 YSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSKSK 247
N+ A +S SS+ + + + D ++ + +E
Sbjct: 229 LPEGLNIYATTASNAEESSWGTYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMH-----N 283
Query: 248 LTMQDLINSYNPYEIHSTP------GVQPINLRRSPDDILITDFFGNVRDIELHSEKINW 301
L + L Y + + P + + G + N+
Sbjct: 284 LQTETLHQQYELVKRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGT------NPANDNF 337
Query: 302 MLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTRELKYKK 352
N+ KP + Q D+ +G K L +
Sbjct: 338 TFADANSLKPPSR---VTNQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHID 394
Query: 353 HP---ISRIISAV 362
+ + +I+ +
Sbjct: 395 NSVILVGKILFGI 407
>sp|Q39119|VPEG_ARATH VACUOLAR PROCESSING ENZYME, GAMMA-ISOZYME PRECURSOR (GAMMA-VPE)
dbj|BAA18924.1| (D61395) gamma-VPE [Arabidopsis thaliana]
Length = 490
Score = 513 bits (1308), Expect = e-144
Identities = 68/373 (18%), Positives = 142/373 (37%), Gaps = 44/373 (11%)
Query: 18 AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRN 77
+S+ WAVL++ S ++NYRH A++ Y+ +++ G+ + I++ + DD A N N
Sbjct: 47 DDDSNSGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYEN 106
Query: 78 LFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPE-NTPASKRLLTNERS 136
PGT+ N+ D+Y + + DY G +V V+ ++ + K + +
Sbjct: 107 PRPGTII-NSPHGKDVY-QGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPND 164
Query: 137 NILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKI 196
+I I+ + HGG G + + L + DL D +++ H Y ++F ++ C++ S++ +
Sbjct: 165 HIFIFYSDHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGL 224
Query: 197 YSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSKSK 247
N+ A +S SS+ + + + D ++ + +E
Sbjct: 225 LPEGLNIYATTASNAEESSWGTYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMH-----N 279
Query: 248 LTMQDLINSYNPYEIHSTP------GVQPINLRRSPDDILITDFFGNVRDIELHSEKINW 301
L + L Y + + P + + G + N+
Sbjct: 280 LQTETLHQQYELVKRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGT------NPANDNF 333
Query: 302 MLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTRELKYKK 352
N+ KP + Q D+ +G K L +
Sbjct: 334 TFADANSLKPPSR---VTNQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHID 390
Query: 353 HP---ISRIISAV 362
+ + +I+ +
Sbjct: 391 NSVILVGKILFGI 403
>emb|CAC18099.1| (AJ251453) putative legumain [Zea mays]
Length = 486
Score = 511 bits (1304), Expect = e-144
Identities = 68/364 (18%), Positives = 130/364 (35%), Gaps = 42/364 (11%)
Query: 19 AESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRNL 78
+ WAVLI+ S ++NYRH A++ Y+ +K+ G+ D II+ + DD A + N
Sbjct: 43 DDGDVGTRWAVLIAGSSGYYNYRHQADICHAYQIMKKGGLKDENIIVFMYDDIAHSPENP 102
Query: 79 FPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPEN-TPASKRLLTNERSN 137
PG + N + D+Y + DY G +V V+ F +L + K + + +
Sbjct: 103 RPGVII-NHPQGGDVYA-GVPKDYTGRDVNVDNFFAVLLGNKTALRGGSGKVVDSGPNDH 160
Query: 138 ILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKIY 197
I ++ + HGG G + L +DL D +++ H Y ++F ++ C++ S++ +
Sbjct: 161 IFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLL 220
Query: 198 SP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSKSKL 248
NV A +S SS+ + + + D ++ + +E + L
Sbjct: 221 PNDINVYATTASNADESSWGTYCPGEFPSPPPEYDTCLGDLYSVAWMEDSDFH-----NL 275
Query: 249 TMQDLINSYNPYEIHSTP-------GVQPINLRRSPDDILITDFFGNVRDIELHSEKINW 301
+ L Y + + + + + G
Sbjct: 276 RTESLKQQYKLVKDRTAVHDTFSYGSHVMQYGALELNVQRLFSYIGTD------PANDGN 329
Query: 302 MLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTRELKYKK 352
+N+ K V Q D+ D K L +
Sbjct: 330 TFIEDNSLPSFSK---AVNQRDADLVYFWQKYRKLADSSHAKNEARKELLEVMAHRSHVD 386
Query: 353 HPIS 356
+
Sbjct: 387 SSVE 390
>ref|NP_071562.1| (NM_022226) protease, cysteine, 1 (legumain) [Rattus norvegicus]
dbj|BAA84750.1| (AB032766) legumain [Rattus norvegicus]
Length = 435
Score = 511 bits (1304), Expect = e-144
Identities = 94/388 (24%), Positives = 161/388 (41%), Gaps = 28/388 (7%)
Query: 1 MIVQFVALLLLNLLQII-----AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKR 55
M + LL L L +W V+++ S W+NYRH A+ Y+ + R
Sbjct: 1 MTWRVAVLLSLVLGAGAVHIGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHR 60
Query: 56 LGIPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRL 115
GIPD QII+M+ DD A N N PG V N D+Y + + DY G +VT E F+ +
Sbjct: 61 NGIPDEQIIVMMYDDIANNEENPTPGVVI-NRPNGTDVY-KGVPKDYTGEDVTPENFLAV 118
Query: 116 LTERVPE--NTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQ 173
L + K L + R ++ +Y T HG G + F + E+L +DL I +++
Sbjct: 119 LRGDEEAVKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNKTIRYMYE 177
Query: 174 HKRYNEILFMVDTCQANSLYTKIYSP-NVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTF 232
HK Y +++F ++ C++ S+ + +V A ++ SSY+ + D + + D ++
Sbjct: 178 HKMYQKMVFYIEACESGSMMNHLPDDIDVYATTAANPNESSYACYYDEERSTYLGDWYSV 237
Query: 233 SNLEFLENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDI 292
+ +E + LT + L Y+ + H+ +S + + F G
Sbjct: 238 NWMEDSDVED-----LTKETLHKQYHLVKSHTNTSHVMQYGNKSISTMKVMQFQGMKHR- 291
Query: 293 ELHSEKINWMLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNLKSFLP-PTRELKYK 351
S I+ PS V ++ L + L
Sbjct: 292 --ASSPISLPPVTHLDLTPS----PDVPLT--ILKRKLLRTNNMKESQVLVGQIQHLLDA 343
Query: 352 KHPISRIISAVVCISFSIGFPYYASKYL 379
+H I + + +V + GF A K+L
Sbjct: 344 RHIIEKSVQKIVSL--LAGFGETAQKHL 369
>emb|CAB64544.1| (AJ131718) legumain-like protease [Zea mays]
Length = 486
Score = 510 bits (1301), Expect = e-143
Identities = 68/364 (18%), Positives = 131/364 (35%), Gaps = 42/364 (11%)
Query: 19 AESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRNL 78
+ + WAVLI+ S ++NYRH A++ Y+ +K+ G+ D II+ + DD A + N
Sbjct: 43 DDGAVGTRWAVLIAGSSGYYNYRHQADICHAYQIMKKGGLKDENIIVFMYDDIAHSPENP 102
Query: 79 FPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPEN-TPASKRLLTNERSN 137
PG + N + D+Y + DY G +V V+ F +L + K + + +
Sbjct: 103 RPGVII-NHPQGGDVYA-GVPKDYTGRDVNVDNFFAVLLGNKTALRGGSGKVVDSGPDDH 160
Query: 138 ILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKIY 197
I ++ + HGG G + L +DL D +++ H Y ++F ++ C++ S++ +
Sbjct: 161 IFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLL 220
Query: 198 SP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSKSKL 248
NV A +S SS+ + + + D ++ + +E + L
Sbjct: 221 PNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLGDLYSVAWMEDSDFH-----NL 275
Query: 249 TMQDLINSYNPYEIHSTP-------GVQPINLRRSPDDILITDFFGNVRDIELHSEKINW 301
+ L Y + + + + + G
Sbjct: 276 RTESLKQQYKLVKDRTAVHDTFSYGSHVMQYGALELNVQHLFSYIGTD------PANDGN 329
Query: 302 MLPGENTTKPSIKRNSFVFQAQNDMQ---------DDGKGFGISNLKSFLPPTRELKYKK 352
+N+ K V Q D+ D K L +
Sbjct: 330 TFIEDNSLPSFSK---AVNQRDADLVYFWQKYRKFADSPPAKSEARKELLEVMAHRSHVD 386
Query: 353 HPIS 356
+
Sbjct: 387 SSVE 390
>gb|AAD04883.1| (AF082347) C13 endopeptidase NP1 precursor [Zea mays]
Length = 485
Score = 510 bits (1300), Expect = e-143
Identities = 67/364 (18%), Positives = 134/364 (36%), Gaps = 42/364 (11%)
Query: 19 AESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRNL 78
+ + WAVLI+ S ++NYRH A++ Y+ +K+ G+ D I++ + DD A + N
Sbjct: 42 DDDAVGTRWAVLIAGSNGYYNYRHQADICHAYQIMKKGGLKDENIVVFMYDDIAHSPENP 101
Query: 79 FPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPEN-TPASKRLLTNERSN 137
PG + N + D+Y + DY G +V V+ F +L + K + + +
Sbjct: 102 RPGVII-NHPQGGDVYA-GVPKDYTGRDVNVDNFFAVLLGNKTALRGGSGKVVDSGPNDH 159
Query: 138 ILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKIY 197
I ++ + HGG G + L +DL D +++ H Y ++F ++ C++ S++ +
Sbjct: 160 IFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLL 219
Query: 198 SP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSKSKL 248
NV A +S SS+ + + + D ++ + +E + L
Sbjct: 220 PNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLGDLYSVAWMEDSDFH-----NL 274
Query: 249 TMQDLINSYNPYEIHSTP-------GVQPINLRRSPDDILITDFFGNVRDIELHSEKINW 301
+ L YN + + + + + G + +
Sbjct: 275 RTESLKQQYNLVKDRTAVQDTFSYGSHVMQYGSLELNVKHLFSYIGT------NPANDDN 328
Query: 302 MLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTRELKYKK 352
+N+ K V Q D+ D + L +
Sbjct: 329 TFIEDNSLPSLSK---AVNQRDADLVYFWQKYRKLADSSPEKNEARRELLEVMAHRSHVD 385
Query: 353 HPIS 356
+
Sbjct: 386 SSVE 389
>dbj|BAB29018.1| (AK013853) data source:SPTR, source key:O14822,
evidence:ISS~homolog to GPI TRANSAMIDASE~putative [Mus
musculus]
Length = 395
Score = 509 bits (1299), Expect = e-143
Identities = 176/391 (45%), Positives = 244/391 (62%), Gaps = 21/391 (5%)
Query: 2 IVQFVALLLLNLLQIIAA----------ESSHTNNWAVLISTSRFWFNYRHTANVLGIYR 51
+ AL LL+L A S HTNNWAVL+ TSRFWFNYRH AN L +YR
Sbjct: 11 VATLAALALLSLGSSAAGHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYR 70
Query: 52 SVKRLGIPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEA 111
SVKRLGIPDS I+LM+ADD ACN+RN P TVF + + L++YG+++E+DY+ Y TVE
Sbjct: 71 SVKRLGIPDSHIVLMLADDMACNARNPKPATVFSHKNMELNVYGDDVEVDYRSYRGTVEN 130
Query: 112 FIRLLTERVPENTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQI 171
F+R+LT VP +TP SKRLL++++SNILIYMTGHGG+GF+KFQD+EE+++ +LADA EQ+
Sbjct: 131 FLRVLTXEVPPSTPRSKRLLSDDKSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQM 190
Query: 172 HQHKRYNEILFMVDTCQANSLYTKIYSPNVLAIGSSEVGTSSYSHHADIDIGVAVIDRFT 231
Q +RYNE+LF++DTCQ S+Y + YSPN++A+ SS+VG S SH D IGV ++DR+T
Sbjct: 191 WQKRRYNELLFIIDTCQGASMYERFYSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYT 250
Query: 232 FSNLEFLENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRD 291
F LEF ++ S+ M DL STPG + +R P ++LITDFFG+VR
Sbjct: 251 FYVLEFW-KEINPASQTNMNDLFQVCPKSLCVSTPGHRTDLFQRDPKNVLITDFFGSVRK 309
Query: 292 IELHSEKINWMLPGENTTKPSIKRNSFVFQAQNDMQ--DDGKGFGISNLKSFLPPTRELK 349
+E+ +EKI+ + S K + + ++ + I + K P R
Sbjct: 310 VEITTEKISLQWDSQV-VDSSSKEDGTAEERMGPLKYAEQLPVAQIIHQK---PKPR--- 362
Query: 350 YKKHPISRIISAVVCISFSIGFPYYASKYLK 380
HP I + + + F Y K++K
Sbjct: 363 -DWHPPGGFILGLWALIIMVFFKTYGIKHMK 392
>pir||S60049 vacuolar processing enzyme (EC 3.4.22.-) isozyme alpha precursor -
Arabidopsis thaliana
Length = 478
Score = 508 bits (1296), Expect = e-143
Identities = 70/371 (18%), Positives = 139/371 (36%), Gaps = 42/371 (11%)
Query: 9 LLLNLLQIIAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIA 68
L + + WAVL++ S ++NYRH A+V Y+ +K+ G+ + I++++
Sbjct: 28 LASKFFR-PTENDDDSTKWAVLVAGSSGYWNYRHQADVCHAYQRLKKGGVKEENIVVLMY 86
Query: 69 DDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPE-NTPAS 127
DD A N N PG + N+ D+Y + DY G EV V+ + ++ +
Sbjct: 87 DDIAENEENPRPGVII-NSPNGEDVY-NGVPKDYTGDEVNVDNLLAVILGNKTALKGGSG 144
Query: 128 KRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTC 187
K + + +I IY + HGG G + + L + DL D +++ + Y ++F ++ C
Sbjct: 145 KVVDSGPNDHIFIYYSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEAC 204
Query: 188 QANSLYTKIYSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFL 238
++ S++ + N+ A +S SS+ + + + D ++ + +E
Sbjct: 205 ESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDS 264
Query: 239 ENRVDSKSKLTMQDLINSYNPYEIHST------PGVQPINLRRSPDDILITDFFGNVRDI 292
E L + L Y + + + F G
Sbjct: 265 EKH-----NLQTETLHEQYELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGT---- 315
Query: 293 ELHSEKINWMLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLP 343
+ N+ EN+ +P + Q D+ +G + K L
Sbjct: 316 --NPADENFTFVNENSIRPPSR---VTNQRDADLVHFWHKYQKAPEGSARKVEAQKQVLE 370
Query: 344 PTRELKYKKHP 354
+ +
Sbjct: 371 AMSHRLHVDNS 381
>emb|CAB64545.1| (AJ131719) legumain-like protease [Zea mays]
Length = 481
Score = 507 bits (1293), Expect = e-142
Identities = 69/366 (18%), Positives = 136/366 (36%), Gaps = 42/366 (11%)
Query: 17 IAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSR 76
AA+ + WAVLI+ S ++NYRH A++ Y+ +K+ G+ D I++ + DD A +
Sbjct: 36 AAADDAVGTRWAVLIAGSNGYYNYRHQADICHAYQIMKKGGLKDENIVVFMYDDIAHSPE 95
Query: 77 NLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPEN-TPASKRLLTNER 135
N PG + N + D+Y + DY G +V V+ F +L + K + +
Sbjct: 96 NPRPGVII-NHPQGGDVYA-GVPKDYTGRDVNVDNFFAVLLGNKTALRGGSGKVVDSGPN 153
Query: 136 SNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTK 195
+I ++ + HGG G + L +DL D +++ H Y ++F ++ C++ S++
Sbjct: 154 DHISVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEACESGSIFEG 213
Query: 196 IYSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSKS 246
+ NV A +S SS+ + + + D ++ + +E +
Sbjct: 214 LLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLGDLYSVAWMEDSDFH----- 268
Query: 247 KLTMQDLINSYNPYEIHSTP-------GVQPINLRRSPDDILITDFFGNVRDIELHSEKI 299
L + L YN + + + + + G +
Sbjct: 269 NLRTESLKQQYNLVKDRTAVQDTFSYGSHVMQYGSLGLNVKHLFSYIGT------NPAND 322
Query: 300 NWMLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTRELKY 350
+ +N+ K V Q D+ D + L +
Sbjct: 323 DNTFIEDNSLPSFSK---AVNQRDADLVYFWQKYRKLADSSPEKNEARRELLEVMAHRSH 379
Query: 351 KKHPIS 356
+
Sbjct: 380 VDSSVE 385
>pir||T12044 probable legumain (EC 3.4.22.34) precursor - kidney bean
emb|CAB17079.1| (Z99957) legumain-like proteinase precursor [Phaseolus vulgaris]
Length = 493
Score = 505 bits (1289), Expect = e-142
Identities = 65/359 (18%), Positives = 129/359 (35%), Gaps = 43/359 (11%)
Query: 18 AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRN 77
A WAVL++ S + NYRH A+V Y+ + + G+ + I++ + DD A + N
Sbjct: 50 AESDEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVVFMYDDIATHELN 109
Query: 78 LFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPE-NTPASKRLLTNERS 136
PG + +N + D+Y + DY G VT F +L + + K + +
Sbjct: 110 PRPGVIINN-PQGPDVYA-GVPKDYTGESVTSHNFFAVLLGDKSKVKGGSGKVINSKPED 167
Query: 137 NILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKI 196
I +Y + HGG G + + L + D D +++ H Y E++ V+ C++ S++ I
Sbjct: 168 RIFVYYSDHGGPGVLGMPNMPYLYAMDFIDVLKKKHASGGYKEMVIYVEACESGSIFEGI 227
Query: 197 YSP--NVLAIGSSEVGTSSYSHHAD-------IDIGVAVIDRFTFSNLEFLENRVDSKSK 247
N+ +S +S+ + + + D ++ + +E E+
Sbjct: 228 MPKDLNIYVTTASNAQENSWGTYCPGMYPPPPPEYITCLGDLYSVAWMEDSESH-----N 282
Query: 248 LTMQDLINSYNPYEIHSTP-------GVQPINLRRSPDDILITDFFGNVRDIELHSEKIN 300
L + + Y + ++ + + + G
Sbjct: 283 LKKESVEQQYQSVKQRTSNFEAYAMGSHVMQYGDANMTAEKLYLYHGFDPATVN------ 336
Query: 301 WMLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTRELKY 350
P N S + V Q ++ + LK + K+
Sbjct: 337 --FPPHNGRLKS--KMEVVNQRDAELLFMWQVYQRSNHLPEKKTDILKQIEEIVKHRKH 391
>sp|P49045|VPE_SOYBN VACUOLAR PROCESSING ENZYME PRECURSOR (VPE)
pir||T07132 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - soybean
dbj|BAA06030.1| (D28876) cysteine proteinase [Glycine max]
Length = 495
Score = 505 bits (1289), Expect = e-142
Identities = 65/365 (17%), Positives = 127/365 (33%), Gaps = 43/365 (11%)
Query: 18 AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRN 77
A WAVL++ S + NYRH A+V Y+ + + G+ + I++ + DD A N N
Sbjct: 52 ADSDEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGLKEENIVVFMYDDIATNELN 111
Query: 78 LFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPE-NTPASKRLLTNERS 136
G + N DLY + DY G VT E ++ + + K + +
Sbjct: 112 PRHGVII-NHPEGEDLYA-GVPKDYTGDNVTTENLFAVILGDKSKLKGGSGKVINSKPED 169
Query: 137 NILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKI 196
I IY + HGG G + + L + D D +++ H Y E++ V+ C++ S++ I
Sbjct: 170 RIFIYYSDHGGPGILGMPNMPYLYAMDFIDVLKKKHASGSYKEMVIYVEACESGSVFEGI 229
Query: 197 YSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSKSK 247
N+ +S +S+ + + + D ++ + +E E
Sbjct: 230 MPKDLNIYVTTASNAQENSWGTYCPGMDPSPPPEYITCLGDLYSVAWMEDSEAH-----N 284
Query: 248 LTMQDLINSYNPYEIHSTP-------GVQPINLRRSPDDILITDFFGNVRDIELHSEKIN 300
L + + Y + ++ + + + G
Sbjct: 285 LKRESVKQQYKSVKQRTSNFNNYAMGSHVMQYGDTNITAEKLYLYQGFDPATVN------ 338
Query: 301 WMLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTRELKYK 351
P +N + + V Q ++ + LK + K+
Sbjct: 339 --FPPQNGRLET--KMEVVNQRDAELFLLWQMYQRSNHQSENKTDILKQIAETVKHRKHI 394
Query: 352 KHPIS 356
+
Sbjct: 395 DGSVE 399
>ref|NP_035305.1| (NM_011175) protease, cysteine, 1; preprolegumain [Mus musculus]
emb|CAA04439.1| (AJ000990) legumain [Mus musculus]
gb|AAF21659.1|AF044266_1 (AF044266) preprolegumain; cysteine Protease [Mus musculus]
Length = 435
Score = 505 bits (1288), Expect = e-142
Identities = 92/388 (23%), Positives = 162/388 (41%), Gaps = 28/388 (7%)
Query: 1 MIVQFVALLLLNLLQII-----AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKR 55
M + LL L L +W V+++ S W+NYRH A+ Y+ + R
Sbjct: 1 MTWRVAVLLSLVLGAGAVPVGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHR 60
Query: 56 LGIPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRL 115
GIPD QII+M+ DD A + N PG V N D+Y + + DY G +VT E F+ +
Sbjct: 61 NGIPDEQIIVMMYDDIANSEENPTPGVVI-NRPNGTDVY-KGVLKDYTGEDVTPENFLAV 118
Query: 116 LTERVPE--NTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQ 173
L + K L + R ++ IY T HG G + F + ++L +DL I +++
Sbjct: 119 LRGDAEAVKGKGSGKVLKSGPRDHVFIYFTDHGATGILVFPN-DDLHVKDLNKTIRYMYE 177
Query: 174 HKRYNEILFMVDTCQANSLYTKIYSP-NVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTF 232
HK Y +++F ++ C++ S+ + NV A ++ SSY+ + D + G + D ++
Sbjct: 178 HKMYQKMVFYIEACESGSMMNHLPDDINVYATTAANPKESSYACYYDEERGTYLGDWYSV 237
Query: 233 SNLEFLENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDI 292
+ +E + LT + L Y+ + H+ +S + + F G
Sbjct: 238 NWMEDSDVED-----LTKETLHKQYHLVKSHTNTSHVMQYGNKSISTMKVMQFQGMKHR- 291
Query: 293 ELHSEKINWMLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNLKS-FLPPTRELKYK 351
S I+ PS V ++ + ++
Sbjct: 292 --ASSPISLPPVTHLDLTPS----PDVPLT--ILKRKLLRTNDVKESQNLIGQIQQFLDA 343
Query: 352 KHPISRIISAVVCISFSIGFPYYASKYL 379
+H I + + +V + GF A ++L
Sbjct: 344 RHVIEKSVHKIVSL--LAGFGETAERHL 369
>sp|P49046|LEGU_CANEN Legumain precursor (Asparaginyl endopeptidase)
pir||JX0344 legumain (EC 3.4.22.34) precursor - jack bean
dbj|BAA06596.1| (D31787) asparaginyl endopeptidase (Legumain) [Canavalia
ensiformis]
Length = 475
Score = 505 bits (1287), Expect = e-142
Identities = 65/390 (16%), Positives = 137/390 (34%), Gaps = 57/390 (14%)
Query: 1 MIVQFVALLLL------------NLLQIIAA--ESSHTNNWAVLISTSRFWFNYRHTANV 46
M++ V L L +++Q+ + WAVL++ S + NYRH A+V
Sbjct: 1 MVMMLVMLSLHGTAARLNRREWDSVIQLPTEPVDDEVGTRWAVLVAGSNGYGNYRHQADV 60
Query: 47 LGIYRSVKRLGIPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYE 106
Y+ + + G+ + I++ + DD A N+ N PG + N + D+Y + DY G +
Sbjct: 61 CHAYQLLIKGGVKEENIVVFMYDDIAYNAMNPRPGVII-NHPQGPDVYA-GVPKDYTGED 118
Query: 107 VTVEAFIRLLTERVPE-NTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLA 165
VT E ++ + + K + +N I I+ + HGG G + +A + + D
Sbjct: 119 VTPENLYAVILGDKSKVKGGSGKVINSNPEDRIFIFYSDHGGPGVLGMPNAPFVYAMDFI 178
Query: 166 DAIEQIHQHKRYNEILFMVDTCQANSLYTKIYSP--NVLAIGSSEVGTSSYSHHA----- 218
D +++ H Y E++ ++ C++ S++ I N+ +S +S+ +
Sbjct: 179 DVLKKKHASGGYKEMVIYIEACESGSIFEGIMPKDLNIYVTTASNAQENSFGTYCPGMNP 238
Query: 219 --DIDIGVAVIDRFTFSNLEFLENRVDSKSKLTMQDLINSYNPYEIHSTP-------GVQ 269
+ + D ++ S +E E L + + Y ++
Sbjct: 239 PPPEEYVTCLGDLYSVSWMEDSETH-----NLKRETVQQQYQSVRKRTSNSNSYRFGSHV 293
Query: 270 PINLRRSPDDILITDFFGNVRDIELHSEKINWMLPGENTTKPSIKRNSFVFQAQNDM--- 326
+ + + G + V Q ++
Sbjct: 294 MQYGDTNITAEKLYLYHGFDPATVNFPPHNGN----------LEAKMEVVNQRDAELLFM 343
Query: 327 ------QDDGKGFGISNLKSFLPPTRELKY 350
+ L+ + +
Sbjct: 344 WQMYQRSNHQPEKKTHILEQITETVKHRNH 373
>gb|AAF89679.1| (AF169973) asparaginyl endopeptidase [Sesamum indicum]
Length = 489
Score = 505 bits (1287), Expect = e-142
Identities = 71/372 (19%), Positives = 133/372 (35%), Gaps = 46/372 (12%)
Query: 13 LLQIIAAE---SSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIAD 69
+++ + WAVL++ S + NYRH A+V Y+ +K+ G+ D II+ + D
Sbjct: 38 IIRWPLDRRETEDNATRWAVLVAGSNGFGNYRHQADVCHAYQILKKGGLRDENIIVFMYD 97
Query: 70 DYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPE-NTPASK 128
D A N N G + N D+Y + DY G +VT E ++ + K
Sbjct: 98 DIAMNELNPRKGVII-NHPTGGDVYA-GVPKDYTGEQVTAENLYAVILGDKSAIKGGSGK 155
Query: 129 RLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQ 188
+ + I IY + HGG G + + L + D + +++ H Y E++ V+ C+
Sbjct: 156 VVDSKPNDRIFIYYSDHGGPGVLGMPNMPYLYANDFIEVLKKKHASGTYKEMVIYVEACE 215
Query: 189 ANSLYTKIYSP--NVLAIGSSEVGTSSYSHHAD-------IDIGVAVIDRFTFSNLEFLE 239
+ S++ + ++ +S SS+ + + + D ++ + +E E
Sbjct: 216 SGSVFEGLMPDDLDIYVTTASNAEESSWGTYCPGMDPPPPPEYITCLGDLYSVAWMEDSE 275
Query: 240 NRVDSKSKLTMQDLINSYNPYEIHSTP-------GVQPINLRRSPDDILITDFFGNVRDI 292
+ L + + Y + ++ +S + + G
Sbjct: 276 SH-----NLKRETVEQQYQQVKERTSNFNTYNAGSHVMEYGNKSIKSEKLYLYQGFDPAT 330
Query: 293 ELHSEKINWMLPGENTTKPSIKRNSFVFQAQNDMQDDGKG---------FGISNLKSFLP 343
E M P EN KP V Q D+ + K
Sbjct: 331 EN-------MPPSENHLKP---HMDVVNQRDADLLFLWERYKRLDGGAKKKSELFKLITD 380
Query: 344 PTRELKYKKHPI 355
K+ I
Sbjct: 381 TMLHRKHMDDSI 392
>emb|CAC43295.1| (AJ309173) putative vacuolar processing enzyme [Beta vulgaris]
Length = 486
Score = 505 bits (1287), Expect = e-142
Identities = 73/362 (20%), Positives = 140/362 (38%), Gaps = 39/362 (10%)
Query: 18 AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRN 77
+ + S WAVLI+ S ++NYRH A+V Y+ +K+ G+ D II+ + DD A + N
Sbjct: 43 SDDDSVGTRWAVLIAGSSGYWNYRHQADVCHAYQVLKKGGLKDENIIVFMYDDIAYDEEN 102
Query: 78 LFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPEN-TPASKRLLTNERS 136
PG + N+ D+Y + DY G +VTV F + + K + +
Sbjct: 103 PRPGVLI-NSPYGHDVYA-GVPKDYTGEDVTVNNFFAAILGNKDAITGGSGKVVNSGPND 160
Query: 137 NILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKI 196
+I I+ + HGG G + L +++L + +++ H Y ++ ++ C++ S++ I
Sbjct: 161 HIFIFYSDHGGAGVLGMPTYPYLYADELIETLKEKHASGTYKSLVVYIEACESGSIFEGI 220
Query: 197 YSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSKSK 247
N+ A +S SS+ + + + D ++ S +E E
Sbjct: 221 LPEGLNIYATTASNAVESSWGTYCPGQDPNVPPEYDTCLGDLYSVSWIEDSERH-----N 275
Query: 248 LTMQDLINSYNPYEIHSTP-----GVQPINLRRSPDDILITDFFGNVRDIELHSEKINWM 302
L + L Y + + + ++ + G + N+
Sbjct: 276 LHTESLKQQYEVVKTKTAEKPFYGSHVMQYGDKELTQDMLYLYMGT------NPNNENYT 329
Query: 303 LPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTRELKYKKH 353
+N+ P+ V Q D+ +G I+ K F+ +
Sbjct: 330 YVDDNSLHPTSSN--AVNQRDADLIHFWNKFRKASEGSQRKINAQKQFMEVMSHRVHLDD 387
Query: 354 PI 355
I
Sbjct: 388 SI 389
>emb|CAC18100.1| (AJ251454) putative legumain [Zea mays]
Length = 485
Score = 505 bits (1287), Expect = e-142
Identities = 68/364 (18%), Positives = 134/364 (36%), Gaps = 42/364 (11%)
Query: 19 AESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRNL 78
+ + WAVLI+ S ++NYRH A++ Y+ +K+ G+ D I++ + DD A + N
Sbjct: 42 DDDAVGTRWAVLIAGSNGYYNYRHQADICHAYQIMKKGGLKDENIVVFMYDDIAHSPENP 101
Query: 79 FPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPEN-TPASKRLLTNERSN 137
PG + N + D+Y + DY G EV V+ F +L + K + + +
Sbjct: 102 RPGVII-NHPQGGDVYA-GVPKDYTGREVNVDNFFAVLLGNKTALRGGSGKVVDSGPNDH 159
Query: 138 ILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKIY 197
I ++ + HGG G + L +DL D +++ H Y ++F ++ C++ S++ +
Sbjct: 160 IFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLL 219
Query: 198 SP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSKSKL 248
NV A +S SS+ + + + D ++ + +E + L
Sbjct: 220 PNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLGDLYSVAWMEDSDFH-----NL 274
Query: 249 TMQDLINSYNPYEIHSTP-------GVQPINLRRSPDDILITDFFGNVRDIELHSEKINW 301
+ L YN + + + + + G + +
Sbjct: 275 RTESLKQQYNLVKDRTAVQDTFSYGSHVMQYGSLELNVKHLFSYIGT------NPANDDN 328
Query: 302 MLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTRELKYKK 352
+N+ K V Q D+ D + L +
Sbjct: 329 TSIEDNSLPSFSK---AVNQRDADLVYFWQKYRKLADSSHEKNEARRELLEVMAHRSHVD 385
Query: 353 HPIS 356
+
Sbjct: 386 SSVE 389
>sp|P49042|VPE_RICCO Vacuolar processing enzyme precursor (VPE)
pir||JQ2387 vacuolar processing enzyme (EC 3.4.22.-) precursor - castor bean
dbj|BAA04225.1| (D17401) precursor of vacuolar processing enzyme [Ricinus communis]
Length = 497
Score = 504 bits (1286), Expect = e-141
Identities = 61/360 (16%), Positives = 128/360 (34%), Gaps = 43/360 (11%)
Query: 17 IAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSR 76
+ + WAVL++ S + NYRH A+V Y+ +++ G+ + II+ + DD A N
Sbjct: 53 VDDDDQLGTRWAVLVAGSMGFGNYRHQADVCHAYQLLRKGGLKEENIIVFMYDDIAKNEL 112
Query: 77 NLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPEN-TPASKRLLTNER 135
N PG + N + D+Y + DY G VT + +L + K + +
Sbjct: 113 NPRPGVII-NHPQGEDVYA-GVPKDYTGEHVTAKNLYAVLLGDKSAVQGGSGKVVDSKPN 170
Query: 136 SNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTK 195
I +Y + HGG G + + L + D + +++ H Y +++ V+ C++ S++
Sbjct: 171 DRIFLYYSDHGGPGVLGMPNLPYLYAMDFIEVLKKKHAAGGYKKMVIYVEACESGSIFEG 230
Query: 196 IYSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSKS 246
I ++ +S SS+ + + + D ++ + +E E+
Sbjct: 231 IMPKDVDIYVTTASNAQESSWGTYCPGMEPSPPPEFTTCLGDLYSVAWMEDSESH----- 285
Query: 247 KLTMQDLINSYNPYEIHSTPG-------VQPINLRRSPDDILITDFFGNVRDIELHSEKI 299
L + + Y+ + ++ +S + F G
Sbjct: 286 NLKKETVKQQYSSVKARTSNYNTYAAGSHVMQYGNQSIKADKLYLFQGFDPASVNFPPNN 345
Query: 300 NWMLPGENTTKPSIKRNSFVFQAQNDMQ---------DDGKGFGISNLKSFLPPTRELKY 350
V Q ++ ++G L+ + +
Sbjct: 346 AH----------LNAPMEVVNQRDAELHFMWQLYKRSENGSEKKKEILQQIKDAIKHRSH 395
>sp|P49044|VPE_VICSA VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) (PROTEINASE B)
pir||S49175 legumain (EC 3.4.22.34) precursor [similarity] - spring vetch
pir||S68984 cysteine proteinase precursor - spring vetch
emb|CAA84383.1| (Z34899) cysteine proteinase [Vicia sativa]
Length = 493
Score = 503 bits (1282), Expect = e-141
Identities = 69/369 (18%), Positives = 132/369 (35%), Gaps = 40/369 (10%)
Query: 13 LLQII-AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDY 71
+ + WA+L++ S ++NYRH ++V Y+ +++ G + II+ + DD
Sbjct: 42 FFREPKNDDDFEGTRWAILLAGSNGYWNYRHQSDVCHAYQLLRKGGSKEENIIVFMYDDI 101
Query: 72 ACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPEN-TPASKRL 130
A N N PG + N D+Y + DY G EV + F L + K +
Sbjct: 102 ASNEENPRPGVII-NKPDGDDVYA-GVPKDYTGAEVHADNFYAALLGNKSALTGGSGKVV 159
Query: 131 LTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQAN 190
+ +I +Y T HGG G + L + DL + +++ H Y ++F ++ C++
Sbjct: 160 DSGPNDHIFVYYTDHGGPGVLGMPVGPYLYASDLNEVLKKKHASGTYKSLVFYLEACESG 219
Query: 191 SLYTKIYSP--NVLAIGSSEVGTSSYSHHAD-------IDIGVAVIDRFTFSNLEFLENR 241
S++ + N+ A +S SS+ ++ + + D ++ + +E E
Sbjct: 220 SIFEGLLPDDLNIYATTASNAEESSWGYYCPGDKPPPPPEYSTCLGDLYSIAWMEDSEVH 279
Query: 242 VDSKSKLTMQDLINSYNPYEIHSTP----GVQPINLRRSPDDILITDFFGNVRDIELHSE 297
L + L Y + + + + G +
Sbjct: 280 -----NLQTESLQQQYKLVKNRTISEPYGSHVMEYGDIGLSKNDLYQYLGT------NPA 328
Query: 298 KINWMLPG--ENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTR 346
N EN+ K V Q D+ +G K L
Sbjct: 329 NDNNSFVDETENSLKLRTPSA-AVNQRDADLIHFWEKFRKAPEGSSQKNEAEKQVLEAMS 387
Query: 347 ELKYKKHPI 355
K+ + +
Sbjct: 388 HRKHIDNSV 396
>ref|NP_176458.1| (NM_104948) beta-VPE [Arabidopsis thaliana]
gb|AAK56243.1|AF367254_1 (AF367254) At1g62710/F23N19_8 [Arabidopsis thaliana]
gb|AAL15381.1| (AY059156) At1g62710/F23N19_8 [Arabidopsis thaliana]
Length = 486
Score = 500 bits (1274), Expect = e-140
Identities = 70/364 (19%), Positives = 132/364 (36%), Gaps = 43/364 (11%)
Query: 19 AESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRNL 78
E WAVL++ S + NYRH A+V Y+ +++ G+ + I++++ DD A + N
Sbjct: 44 DEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVVLMYDDIANHPLNP 103
Query: 79 FPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTER-VPENTPASKRLLTNERSN 137
PGT+ N D+Y + DY G VT F +L + K + + +
Sbjct: 104 RPGTLI-NHPDGDDVYA-GVPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPNDH 161
Query: 138 ILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKIY 197
I +Y HGG G + + + + D + +++ H Y E++ V+ C++ S++ I
Sbjct: 162 IFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACESGSIFEGIM 221
Query: 198 SP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSKSKL 248
N+ +S SSY + + + D ++ + +E E L
Sbjct: 222 PKDLNIYVTTASNAQESSYGTYCPGMNPSPPSEYITCLGDLYSVAWMEDSETH-----NL 276
Query: 249 TMQDLINSYNPYEIHSTPG-------VQPINLRRSPDDILITDFFGNVRDIELHSEKINW 301
+ + Y+ ++ ++ S + + G
Sbjct: 277 KKETIKQQYHTVKMRTSNYNTYSGGSHVMEYGNNSIKSEKLYLYQGFDPATVNLPLN--- 333
Query: 302 MLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTRELKYKK 352
P + V Q D+ +DG LK TR K+
Sbjct: 334 -------ELPVKSKIGVVNQRDADLLFLWHMYRTSEDGSRKKDDTLKELTETTRHRKHLD 386
Query: 353 HPIS 356
+
Sbjct: 387 ASVE 390
>gb|AAF89646.1|AF169019_1 (AF169019) seed maturation protein PM40 [Glycine max]
Length = 496
Score = 497 bits (1267), Expect = e-139
Identities = 58/367 (15%), Positives = 128/367 (34%), Gaps = 44/367 (11%)
Query: 17 IAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSR 76
++ WAVL++ S + NYRH A+V Y+ + + G+ + I++ + DD A +
Sbjct: 51 ADSDHEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGLKEENIVVFMYDDIATDEL 110
Query: 77 NLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPE-NTPASKRLLTNER 135
N PG + N D+Y + DY G VT + ++ + + K + +
Sbjct: 111 NPRPGVII-NHPEGQDVYA-GVPKDYTGENVTAQNLFAVILGDKNKVKGGSGKVINSKPE 168
Query: 136 SNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANS-LYT 194
I IY + HGG G + + L + D + +++ H Y +++ V+ C++ + +
Sbjct: 169 DRIFIYYSDHGGPGVLGMPNMPYLYAMDFIEVLKKKHASGGYKKMVIYVEACESGNHVLK 228
Query: 195 KIYSPN--VLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSK 245
I + + +S +S+ + + + D ++ + +E E
Sbjct: 229 GIMPKDLQIYVTTASNAQENSWGTYCPGMDPSPPPEYITCLGDLYSVAWMEDSETH---- 284
Query: 246 SKLTMQDLINSYNPYEIHSTP-------GVQPINLRRSPDDILITDFFGNVRDIELHSEK 298
L + + Y + ++ + + + G
Sbjct: 285 -NLKRESVKQQYKSVKQRTSNFNNYAMGSHVMQYGDTNITAEKLYLYQGFDPAAVN---- 339
Query: 299 INWMLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTRELK 349
P +N + + V Q ++ + LK + K
Sbjct: 340 ----FPPQNGRLET--KMEVVNQRDAELFFMWQMYQRSNHQPEKKTDILKQIAETVKHRK 393
Query: 350 YKKHPIS 356
+ +
Sbjct: 394 HIDGSVE 400
>pir||T00731 hypothetical protein F22O13.26 - Arabidopsis thaliana
Length = 428
Score = 496 bits (1265), Expect = e-139
Identities = 151/348 (43%), Positives = 221/348 (63%), Gaps = 6/348 (1%)
Query: 2 IVQFVALLLLNLLQIIAAESSHTNNWAVLISTSRFW----FNYRHTANVLGIYRSVKRLG 57
I+ V LL + + + HTNNWAVL+ TSRF ++LG+ R+VKRLG
Sbjct: 3 ILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFCSLHSLVLTFIFSLLGVSRTVKRLG 62
Query: 58 IPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLT 117
IPD +IILM+ADD ACN+RN +P VF+N + L+LYG+ +E+DY+GYEVTVE F+R+LT
Sbjct: 63 IPDERIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLT 122
Query: 118 ERVPENTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRY 177
R P SKRLL++E S+IL+YMTGHGGD F+KFQDAEEL S DLADA++Q+ + +R+
Sbjct: 123 GRHENAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRF 182
Query: 178 NEILFMVDTCQANSLYTKIYSPNVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSNLEF 237
E++ MVDTCQA +L+ ++ SP VLAIGSS G +SYSHH D DIGV+V+DRFT+ L F
Sbjct: 183 KELMIMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAF 242
Query: 238 LENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIELHSE 297
E R++ ++ L SY+P + ST + + ++ +T+FFG+V + +
Sbjct: 243 FE-RLNIYDNASLNSLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHT-D 300
Query: 298 KINWMLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNLKSFLPPT 345
+ + + F +++D++++ + I N + T
Sbjct: 301 SAYKAFSSKISERKINSEMPFNQLSEHDLKEELENTNIPNDELIAEVT 348
>emb|CAB16318.1| (Z99174) cysteine proteinase precursor [Vicia narbonensis]
Length = 488
Score = 494 bits (1259), Expect = e-138
Identities = 62/361 (17%), Positives = 127/361 (35%), Gaps = 45/361 (12%)
Query: 17 IAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSR 76
E WAVL++ S + NYRH A+V Y+ + + G+ + I++ + DD A N
Sbjct: 44 ADVEDEMGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVVFMYDDIAYNEM 103
Query: 77 NLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPEN-TPASKRLLTNER 135
N PG + N + ++Y + + DY G VT E F ++ + + K + +
Sbjct: 104 NPRPGVII-NHPQGPNVY-DGVPKDYNGDFVTAENFYAVILGDKSKVRGGSGKVINSKAE 161
Query: 136 SNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTK 195
I IY + HGG G + + + + D D +++ H Y +++ V+ C++ S++
Sbjct: 162 DRIFIYCSDHGGPGVLGMPNMPYVYAMDFIDVLKKKHASGGYKKMVIYVEACESGSIFEG 221
Query: 196 IYSP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSKS 246
I NV +S +S+ + + + D ++ + +E E
Sbjct: 222 IMPKDINVYVTTASNAQENSWGTYCPGVDPAPPPEYITCLGDLYSVAWMEDSETH----- 276
Query: 247 KLTMQDLINSYNPYEIHSTPG-------VQPINLRRSPDDILITDFFGNVRDIELHSEKI 299
L + + Y ++ + D + + G S
Sbjct: 277 NLKRETVKQQYMSVRERTSNYKNYPLGSHVMQYGDTNITDEKLYLYHGFDPADGEPSSNN 336
Query: 300 NWMLPGENTTKPSIKRNSFVFQAQND----------MQDDGKGFGISNLKSFLPPTRELK 349
+ + V Q + + + L+ +
Sbjct: 337 DI----------LEAKMEVVNQRDAEILFMWHMYQRLDHQTEKKKD-TLEKISETVKHRN 385
Query: 350 Y 350
+
Sbjct: 386 H 386
>pir||S60050 vacuolar processing enzyme (EC 3.4.22.-) isozyme beta precursor -
Arabidopsis thaliana
dbj|BAA09615.1| (D61394) beta-VPE [Arabidopsis thaliana]
Length = 484
Score = 489 bits (1248), Expect = e-137
Identities = 70/364 (19%), Positives = 132/364 (36%), Gaps = 45/364 (12%)
Query: 19 AESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRNL 78
E WAVL++ S + NYRH A+V Y+ +++ G+ + I++++ DD A + N
Sbjct: 44 DEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVVLMYDDIANHPLNP 103
Query: 79 FPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTER-VPENTPASKRLLTNERSN 137
PGT+ N D+Y + DY G VT F +L + K + + +
Sbjct: 104 RPGTLI-NHPDGDDVYA-GVPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPNDH 161
Query: 138 ILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKIY 197
I +Y HGG G + + + + D + +++ H Y E++ V+ C++ S++ I
Sbjct: 162 IFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACESGSIFEGIM 221
Query: 198 SP--NVLAIGSSEVGTSSYSHHA-------DIDIGVAVIDRFTFSNLEFLENRVDSKSKL 248
N+ +S SSY + + + D ++ + +E E L
Sbjct: 222 PKDLNIYVTTASNAQESSYGTYCPGMNPSPPSEYITCLGDLYSVAWMEDSETH-----NL 276
Query: 249 TMQDLINSYNPYEIHSTPG-------VQPINLRRSPDDILITDFFGNVRDIELHSEKINW 301
+ + Y+ + ++ S + + G
Sbjct: 277 KKETIKQQYHT--MRTSNYNTYSGGSHVMEYGNNSIKSEKLYLYQGFEPATVNLPLN--- 331
Query: 302 MLPGENTTKPSIKRNSFVFQAQNDM---------QDDGKGFGISNLKSFLPPTRELKYKK 352
P+ + V Q D+ +DG LK TR K+
Sbjct: 332 -------ELPAKSKIGVVNQRDADLLFLWHMHRTSEDGSRKKDDTLKELTETTRHRKHLD 384
Query: 353 HPIS 356
+
Sbjct: 385 ASVE 388
>dbj|BAA09530.1| (D55696) cysteine protease [Homo sapiens]
Length = 433
Score = 487 bits (1242), Expect = e-136
Identities = 84/372 (22%), Positives = 155/372 (41%), Gaps = 24/372 (6%)
Query: 1 MIVQFVALLLLNLLQII---AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLG 57
M+ + L + L +WAV+++ S W+NYRH A+ Y+ + R G
Sbjct: 1 MVWKVAVFLSVALGIGAVPIDDPEDGGKHWAVIVAGSNGWYNYRHQADACHAYQIIHRNG 60
Query: 58 IPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLT 117
IPD QI++M+ DD A + N PG V N D+Y + + DY G +VT + F+ +L
Sbjct: 61 IPDEQIVVMMYDDIAYSEDNPTPGIVI-NRPNGTDVY-QGVPKDYTGEDVTPQNFLAVLR 118
Query: 118 ERVPE--NTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHK 175
+ K L + + ++ IY T HG G + F + E+L +DL + I +++HK
Sbjct: 119 GDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYKHK 177
Query: 176 RYNEILFMVDTCQANSLYTKIYSP-NVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSN 234
Y +++F ++ C++ S+ + NV A ++ SSY+ + D + D ++ +
Sbjct: 178 MYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNW 237
Query: 235 LEFLENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIEL 294
+E + LT + L Y+ + H+ ++ + + F G R
Sbjct: 238 MEDSDVED-----LTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRK--- 289
Query: 295 HSEKINWMLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNL-KSFLPPTRELKYKKH 353
S + PS V M+ + + +H
Sbjct: 290 ASSPVPLPPVTHLDLTPS----PDVPLT--IMKRKLMNTNDLEESRQLTEEIQRHLDARH 343
Query: 354 PISRIISAVVCI 365
I + + +V +
Sbjct: 344 LIEKSVRKIVSL 355
>ref|XP_007399.4| (XM_007399) legumain [Homo sapiens]
Length = 401
Score = 487 bits (1241), Expect = e-136
Identities = 83/372 (22%), Positives = 154/372 (41%), Gaps = 24/372 (6%)
Query: 1 MIVQFVALLLLNLLQII---AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLG 57
M+ + L + L +W V+++ S W+NYRH A+ Y+ + R G
Sbjct: 1 MVWKVAVFLSVALGIGAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNG 60
Query: 58 IPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLT 117
IPD QI++M+ DD A + N PG V N D+Y + + DY G +VT + F+ +L
Sbjct: 61 IPDEQIVVMMYDDIAYSEDNPTPGIVI-NRPNGTDVY-QGVPKDYTGEDVTPQNFLAVLR 118
Query: 118 ERVPE--NTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHK 175
+ K L + + ++ IY T HG G + F + E+L +DL + I +++HK
Sbjct: 119 GDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYKHK 177
Query: 176 RYNEILFMVDTCQANSLYTKIYSP-NVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSN 234
Y +++F ++ C++ S+ + NV A ++ SSY+ + D + D ++ +
Sbjct: 178 MYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNW 237
Query: 235 LEFLENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIEL 294
+E + LT + L Y+ + H+ ++ + + F G R
Sbjct: 238 MEDSDVED-----LTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRK--- 289
Query: 295 HSEKINWMLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNL-KSFLPPTRELKYKKH 353
S + PS V M+ + + +H
Sbjct: 290 ASSPVPLPPVTHLDLTPS----PDVPLT--IMKRKLMNTNDLEESRQLTEEIQRHLDARH 343
Query: 354 PISRIISAVVCI 365
I + + +V +
Sbjct: 344 LIEKSVRKIVSL 355
>pir||T10944 cysteine proteinase (EC 3.4.22.-) precursor - spring vetch
emb|CAA07639.1| (AJ007743) cysteine proteinase precursor [Vicia sativa]
Length = 503
Score = 485 bits (1238), Expect = e-136
Identities = 58/362 (16%), Positives = 125/362 (34%), Gaps = 46/362 (12%)
Query: 17 IAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSR 76
E WAVL++ S + NYRH A+V Y+ + + G+ + I++ + DD A +
Sbjct: 58 ADVEDEIGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVVFMYDDIAYSEF 117
Query: 77 NLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPEN-TPASKRLLTNER 135
N PG + N + ++Y + + DY G VT + ++ + + K + +
Sbjct: 118 NPRPGVII-NHPQGPNVY-DGVPKDYTGDFVTADNLYAVILGDKSKVRGGSGKVINSKAE 175
Query: 136 SNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTK 195
I IY + HGG G + + + + D D +++ H + Y +++ V+ C++ S++
Sbjct: 176 DRIFIYYSDHGGPGVLGMPNMPYVYAMDFIDVLKKKHASRGYQQMVIYVEACESGSVFQG 235
Query: 196 IYSPNV--LAIGSSEVGTSSYSHHA--------DIDIGVAVIDRFTFSNLEFLENRVDSK 245
I ++ +S SS+ + + + D ++ + +E E
Sbjct: 236 IMPKDIDVYVTTASNAEESSWGTYLVPGVYPASPPEYITCLGDLYSVAWMEDSETH---- 291
Query: 246 SKLTMQDLINSYNPYEIHSTP-------GVQPINLRRSPDDILITDFFGNVRDIELHSEK 298
L + L + + + + D + + G
Sbjct: 292 -NLKRETLKQQFASVKERTLNNNNYGLGSHVTEYGDTNITDEKLYLYHGFDPASVNLPPN 350
Query: 299 INWMLPGENTTKPSIKRNSFVFQAQND----------MQDDGKGFGISNLKSFLPPTREL 348
+ V Q + + + LK +
Sbjct: 351 NGR----------LESKMEVVNQRDAEILFMWQMYQRLDHQSEKKRDI-LKKISETVKHR 399
Query: 349 KY 350
+
Sbjct: 400 NH 401
>gb|AAH03061.1|AAH03061 (BC003061) protease, cysteine, 1 (legumain) [Homo sapiens]
Length = 433
Score = 485 bits (1237), Expect = e-136
Identities = 83/372 (22%), Positives = 154/372 (41%), Gaps = 24/372 (6%)
Query: 1 MIVQFVALLLLNLLQII---AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLG 57
M+ + L + L +W V+++ S W+NYRH A+ Y+ + R G
Sbjct: 1 MVWKVAVFLSVALGIGAIPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNG 60
Query: 58 IPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLT 117
IPD QI++M+ DD A + N PG V N D+Y + + DY G +VT + F+ +L
Sbjct: 61 IPDEQIVVMMYDDIAYSEDNPTPGIVI-NRPNGTDVY-QGVPKDYTGEDVTPQNFLAVLR 118
Query: 118 ERVPE--NTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHK 175
+ K L + + ++ IY T HG G + F + E+L +DL + I +++HK
Sbjct: 119 GDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYKHK 177
Query: 176 RYNEILFMVDTCQANSLYTKIYSP-NVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSN 234
Y +++F ++ C++ S+ + NV A ++ SSY+ + D + D ++ +
Sbjct: 178 MYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNW 237
Query: 235 LEFLENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIEL 294
+E + LT + L Y+ + H+ ++ + + F G R
Sbjct: 238 MEDSDVED-----LTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRK--- 289
Query: 295 HSEKINWMLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNL-KSFLPPTRELKYKKH 353
S + PS V M+ + + +H
Sbjct: 290 ASSPVPLPPVTHLDLTPS----PDVPLT--IMKRKLMNTNDLEESRQLTEEIQRHLDARH 343
Query: 354 PISRIISAVVCI 365
I + + +V +
Sbjct: 344 LIEKSVRKIVSL 355
>ref|NP_005597.1| (NM_005606) legumain; protease, cysteine, 1 (legumain) [Homo
sapiens]
sp|Q99538|LEGU_HUMAN Legumain precursor (Asparaginyl endopeptidase)
emb|CAA70989.1| (Y09862) legumain [Homo sapiens]
gb|AAH08004.1|AAH08004 (BC008004) protease, cysteine, 1 (legumain) [Homo sapiens]
Length = 433
Score = 485 bits (1237), Expect = e-136
Identities = 83/372 (22%), Positives = 154/372 (41%), Gaps = 24/372 (6%)
Query: 1 MIVQFVALLLLNLLQII---AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLG 57
M+ + L + L +W V+++ S W+NYRH A+ Y+ + R G
Sbjct: 1 MVWKVAVFLSVALGIGAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNG 60
Query: 58 IPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLT 117
IPD QI++M+ DD A + N PG V N D+Y + + DY G +VT + F+ +L
Sbjct: 61 IPDEQIVVMMYDDIAYSEDNPTPGIVI-NRPNGTDVY-QGVPKDYTGEDVTPQNFLAVLR 118
Query: 118 ERVPE--NTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHK 175
+ K L + + ++ IY T HG G + F + E+L +DL + I +++HK
Sbjct: 119 GDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYKHK 177
Query: 176 RYNEILFMVDTCQANSLYTKIYSP-NVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSN 234
Y +++F ++ C++ S+ + NV A ++ SSY+ + D + D ++ +
Sbjct: 178 MYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNW 237
Query: 235 LEFLENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIEL 294
+E + LT + L Y+ + H+ ++ + + F G R
Sbjct: 238 MEDSDVED-----LTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRK--- 289
Query: 295 HSEKINWMLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNL-KSFLPPTRELKYKKH 353
S + PS V M+ + + +H
Sbjct: 290 ASSPVPLPPVTHLDLTPS----PDVPLT--IMKRKLMNTNDLEESRQLTEEIQRHLDARH 343
Query: 354 PISRIISAVVCI 365
I + + +V +
Sbjct: 344 LIEKSVRKIVSL 355
>gb|AAF99765.1|AC003981_15 (AC003981) F22O13.24 [Arabidopsis thaliana]
Length = 407
Score = 484 bits (1234), Expect = e-135
Identities = 149/344 (43%), Positives = 214/344 (61%), Gaps = 19/344 (5%)
Query: 2 IVQFVALLLLNLLQIIAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDS 61
I+ V LL + + + HTNNWAVL+ TSRFW +VKRLGIPD
Sbjct: 3 ILTLVMLLCYSFVSSTGDTTIHTNNWAVLVCTSRFW--------------TVKRLGIPDE 48
Query: 62 QIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVP 121
+IILM+ADD ACN+RN +P VF+N + L+LYG+ +E+DY+GYEVTVE F+R+LT R
Sbjct: 49 RIILMLADDMACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHE 108
Query: 122 ENTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEIL 181
P SKRLL++E S+IL+YMTGHGGD F+KFQDAEEL S DLADA++Q+ + +R+ E++
Sbjct: 109 NAVPRSKRLLSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELM 168
Query: 182 FMVDTCQANSLYTKIYSPNVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSNLEFLENR 241
MVDTCQA +L+ ++ SP VLAIGSS G +SYSHH D DIGV+V+DRFT+ L F E R
Sbjct: 169 IMVDTCQAATLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFE-R 227
Query: 242 VDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIELHSEKINW 301
++ L SY+P + ST + + ++ +T+FFG+V + +
Sbjct: 228 LNIYDNA---SLNRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHT-DSAYK 283
Query: 302 MLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNLKSFLPPT 345
+ + + F +++D++++ + I N + T
Sbjct: 284 AFSSKISERKINSEMPFNQLSEHDLKEELENTNIPNDELIAEVT 327
>dbj|BAB69947.1| (AB060129) legumain [Bos taurus]
Length = 433
Score = 478 bits (1220), Expect = e-134
Identities = 90/372 (24%), Positives = 155/372 (41%), Gaps = 24/372 (6%)
Query: 1 MIVQFVALLLLNLLQII---AAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLG 57
MI +F LL L L +W V+++ S W+NYRH A+ Y+ V R G
Sbjct: 1 MIWEFTVLLSLVLGTGAVPLEDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIVHRNG 60
Query: 58 IPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLT 117
IPD QII+M+ DD A + N PG V N D+Y + + DY G +VT + F+ +L
Sbjct: 61 IPDEQIIVMMYDDIANSEDNPTPGIVI-NRPNGSDVY-QGVLKDYTGEDVTPKNFLAVLR 118
Query: 118 ERVPE--NTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHK 175
+ K L + R ++ +Y T HG G + F + E+L +DL + I +++HK
Sbjct: 119 GDAEAVKGVGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNETIRYMYEHK 177
Query: 176 RYNEILFMVDTCQANSLYTKIYSP-NVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSN 234
Y +++F ++ C++ S+ + NV A ++ SSY+ + D + D ++ +
Sbjct: 178 MYQKMVFYIEACESGSMMNHLPPDINVYATTAANPRESSYACYYDEQRSTFLGDWYSVNW 237
Query: 235 LEFLENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIEL 294
+E + LT + L Y + H+ +S + + F G
Sbjct: 238 MEDSDVED-----LTKETLHKQYQLVKSHTNTSHVMQYGNKSISAMKLMQFQGLKHQ--- 289
Query: 295 HSEKINWMLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNLKSFLPP-TRELKYKKH 353
S I+ PS V + M+ L ++
Sbjct: 290 ASSPISLPAVSRLDLTPS----PEVPLS--IMKRKLMSTNDLQESRRLVQKIDRHLEARN 343
Query: 354 PISRIISAVVCI 365
I + + +V +
Sbjct: 344 IIEKSVRKIVTL 355
>emb|CAB42655.1| (AJ238882) putative preprolegumain [Vicia narbonensis]
Length = 380
Score = 466 bits (1187), Expect = e-130
Identities = 56/336 (16%), Positives = 120/336 (35%), Gaps = 41/336 (12%)
Query: 45 NVLGIYRSVKRLGIPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKG 104
+V Y+ +++ G+ + II+ + DD A + N PG + N+ ++Y E + DY G
Sbjct: 1 DVCHAYQLLRKGGLKEENIIVFMYDDIAYSEENPRPGVII-NSPHGENVY-EGVPKDYTG 58
Query: 105 YEVTVEAFIRLLTERVPEN-TPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSED 163
+VTV F L + K + + I ++ + HGG G + + + + D
Sbjct: 59 EDVTVGNFFAALLGNKSALSGGSGKVVDSGPNDRIFVFYSDHGGPGVLGMPTSPYMYASD 118
Query: 164 LADAIEQIHQHKRYNEILFMVDTCQANSLYTKIYSP--NVLAIGSSEVGTSSYSHHA--- 218
L + ++ H Y ++F ++ C++ S++ + N+ A ++ SS+ +
Sbjct: 119 LVEVLKIKHAAGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTAANAEESSWGTYCPGE 178
Query: 219 ----DIDIGVAVIDRFTFSNLEFLENRVDSKSKLTMQDLINSYNPYEIHSTP------GV 268
+ + D ++ + +E + L + L Y + ++
Sbjct: 179 NPSPPPEYETCLADLYSVAWMEDSDIH-----NLQTETLHQQYELVKERTSNGNSNYGSH 233
Query: 269 QPINLRRSPDDILITDFFGNVRDIELHSEKINWMLPGENTTKPSIKRNSFVFQAQNDM-- 326
+ + G+ + N+ G N+ P K + Q D+
Sbjct: 234 VMQYGDIELSKDSLFLYLGS------NPSNENFTFVGRNSLVPPSK---AINQRDADLIH 284
Query: 327 -------QDDGKGFGISNLKSFLPPTRELKYKKHPI 355
G + K L + I
Sbjct: 285 FWDKFRKAPQGSPRKAAAQKEVLEAMSHRMHIDDSI 320
>emb|CAB42651.1| (AJ238881) putative preprolegumain [Nicotiana tabacum]
Length = 419
Score = 452 bits (1153), Expect = e-126
Identities = 55/335 (16%), Positives = 113/335 (33%), Gaps = 44/335 (13%)
Query: 42 HTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEID 101
H A+V Y+ +KR G+ D I++ + DD A + N PG + N D+Y + D
Sbjct: 1 HQADVCHAYQILKRGGLKDENIVVFMYDDIAKSELNPRPGVII-NHPNGSDVYA-GVPKD 58
Query: 102 YKGYEVTVEAFIRLLTERVPE-NTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELS 160
Y G VT +L + K + + I +Y + HGG G + + L
Sbjct: 59 YTGEHVTAANLYAVLLGDKSAVKGGSGKIVDSKPNDRIFLYYSDHGGPGVLGMPNMPFLY 118
Query: 161 SEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKIYSP--NVLAIGSSEVGTSSYSHHA 218
++D + +++ H Y E++ ++ C++ S++ + N+ +S SS+ +
Sbjct: 119 AKDFIEVLKKKHAAGTYKEMVLYIEACESGSVFEGMMPEDLNIYVTTASNAEESSWGTYC 178
Query: 219 D-------IDIGVAVIDRFTFSNLEFLENRVDSKSKLTMQDLINSYNPYEIHSTP----- 266
+ + D ++ + +E E+ L + + Y + ++
Sbjct: 179 PGMDPPPPPEYITCLGDLYSVAWMEDSESH-----NLKKETIKQQYEKVKERTSNFNNYN 233
Query: 267 --GVQPINLRRSPDDILITDFFGNVRDIELHSEKINWMLPGENTTKPSIKRNSFVFQAQN 324
+ + + G S K + V Q
Sbjct: 234 AGSHVMEYGSKEIKPEKVYLYQGFDPATANLSAN-----------KIAFAHVEVVNQRDA 282
Query: 325 DMQDDGKGFGISNL---------KSFLPPTRELKY 350
D+ + + K K+
Sbjct: 283 DLLFLWERYKELADNSLEKAKLRKEITDTMLHRKH 317
>emb|CAB71158.1| (AJ250582) asparaginyl endopeptidase [Schistosoma mansoni]
Length = 429
Score = 449 bits (1144), Expect = e-125
Identities = 89/367 (24%), Positives = 159/367 (43%), Gaps = 37/367 (10%)
Query: 1 MIVQFVALLLLNLLQI---------IAAES-SHTNNWAVLISTSRFWFNYRHTANVLGIY 50
M+ + +L++L + ++ E+ S N WAVL++ S + NYRH A+V Y
Sbjct: 2 MLFSLFLISILHILLVKCQLDTNYEVSDETVSDNNKWAVLVAGSNGYPNYRHQADVCHAY 61
Query: 51 RSVKRLGIPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVE 110
++ GI II M+ DD A N N FPG +F++ + D Y E + IDY+G +V +
Sbjct: 62 HVLRSKGIKPEHIITMMYDDIAYNLMNPFPGKLFNDYNHK-DWY-EGVVIDYRGKKVNSK 119
Query: 111 AFIRLLTERVPENTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQ 170
F+++L K L + + ++ IY T HG G I F D +EL ++ ++
Sbjct: 120 TFLKVLKGDKSAG---GKVLKSGKNDDVFIYFTDHGAPGLIAFPD-DELYAKQFMSTLKY 175
Query: 171 IHQHKRYNEILFMVDTCQANSLYTKIYSPN--VLAIGSSEVGTSSYSHHA-DIDIGVAVI 227
+H HKRY++++ ++ C++ S++ +I N + A ++ SSY D I +
Sbjct: 176 LHSHKRYSKLVIYIEACESGSMFQRILPSNLSIYATTAASPTESSYGTFCDDPTITTCLA 235
Query: 228 DRFTFSNLEFLENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFG 287
D +++ + + LT + L Y + + + +++F G
Sbjct: 236 DLYSYDWIVDSQTH-----HLTQRTLDQQYKEVKRETNLSHVQRYGDTRMGKLHVSEFQG 290
Query: 288 NVRDIELHSEKINWMLPGENTTKPSIKRNSFV--FQAQNDMQDDGKGFGISNLKSFLPPT 345
+ EN P R+S + + + KSFL
Sbjct: 291 SRDKS-----------STENDEPPMKPRHSIASRDIPLHTLHRQIMMTNNAEDKSFLMQI 339
Query: 346 RELKYKK 352
LK K+
Sbjct: 340 LGLKLKR 346
>gb|AAF45703.1| (AE003422) EG:133E12.3 gene product [Drosophila melanogaster]
Length = 322
Score = 445 bits (1134), Expect = e-124
Identities = 168/290 (57%), Positives = 213/290 (72%), Gaps = 1/290 (0%)
Query: 17 IAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSR 76
A S+HTNNWAVL+ SRFWFNYRH ANVL IYRSVKRLGIPDSQIILMIADD ACN+R
Sbjct: 33 AAQRSTHTNNWAVLVDASRFWFNYRHVANVLSIYRSVKRLGIPDSQIILMIADDMACNAR 92
Query: 77 NLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPENTPASKRLLTNERS 136
N PG V++NA++ +++YG+++E+DY+GYEVTVE F+RLLT R T SK+LL++ S
Sbjct: 93 NPRPGQVYNNANQHINVYGDDVEVDYRGYEVTVENFVRLLTGRTQNGTARSKKLLSDAGS 152
Query: 137 NILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKI 196
N+LIY+TGHGGDGF+KFQD+EE++S++LAD I+Q+ + KRYNE+ FMVDTCQA SLY K
Sbjct: 153 NVLIYLTGHGGDGFLKFQDSEEITSQELADGIQQMWEKKRYNELFFMVDTCQAASLYEKF 212
Query: 197 YSPNVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSNLEFLENRVDSKSKLTMQDLINS 256
SPNVLA+ SS VG S SHH D IGV +IDR+T+ LEFLE V SK T+ + +
Sbjct: 213 TSPNVLAVASSLVGEDSLSHHVDPSIGVYMIDRYTYYALEFLEK-VQPFSKRTIGEFLQV 271
Query: 257 YNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIELHSEKINWMLPGE 306
ST GV+ R P + ITDFFG +R + +++IN L E
Sbjct: 272 CPKRVCISTVGVRKDLYPRDPHKVPITDFFGAIRPTRVSTDRINVTLANE 321
>sp|P09841|HGLB_SCHMA Hemoglobinase precursor (Antigen SM32)
pir||A60145 hemoglobinase (EC 3.4.-.-) precursor - fluke (Schistosoma mansoni)
Length = 429
Score = 445 bits (1133), Expect = e-124
Identities = 88/367 (23%), Positives = 159/367 (42%), Gaps = 37/367 (10%)
Query: 1 MIVQFVALLLLNLLQI---------IAAES-SHTNNWAVLISTSRFWFNYRHTANVLGIY 50
M+ + +L++L + ++ E+ S N WAVL++ S + NYRH A+V Y
Sbjct: 2 MLFSLFLISILHILLVKCQLDTNYEVSDETVSDNNKWAVLVAGSNGYPNYRHQADVCHAY 61
Query: 51 RSVKRLGIPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVE 110
++ GI II M+ DD A N N FPG +F++ + D Y E + IDY+G V +
Sbjct: 62 HVLRSKGIKPEHIITMMYDDIAYNLMNPFPGKLFNDYNHK-DWY-EGVVIDYRGKNVNSK 119
Query: 111 AFIRLLTERVPENTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQ 170
F+++L K L + + ++ IY T HG G I F D +EL +++ ++
Sbjct: 120 TFLKVLKGDKSAG---GKVLKSGKNDDVFIYFTDHGAPGLIAFPD-DELYAKEFMSTLKY 175
Query: 171 IHQHKRYNEILFMVDTCQANSLYTKIYSPN--VLAIGSSEVGTSSYSHHA-DIDIGVAVI 227
+H HKRY++++ ++ ++ S++ +I N + A ++ SYS D I +
Sbjct: 176 LHSHKRYSKLVIYIEANESGSMFQQILPSNLSIYATTAANSTECSYSTFCGDPTITTCLA 235
Query: 228 DRFTFSNLEFLENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFG 287
D ++++ + + LT + L Y + + + +++F G
Sbjct: 236 DLYSYNWIVDSQTH-----HLTQRTLDQQYKEVKRETDLSHVQRYGDTRMGKLYVSEFQG 290
Query: 288 NVRDIELHSEKINWMLPGENTTKPSIKRNSFV--FQAQNDMQDDGKGFGISNLKSFLPPT 345
+ EN P R+S + + + KSFL
Sbjct: 291 SRDKS-----------SSENDEPPMKPRHSIASRDIPLHTLHRQIMMTNNAEDKSFLMQI 339
Query: 346 RELKYKK 352
LK K+
Sbjct: 340 LGLKLKR 346
>pir||T13411 hypothetical protein 133E12.3 - fruit fly (Drosophila melanogaster)
emb|CAA15687.1| (AL009192) /prediction=(method:''genefinder'', version:''084'',
score:''46.36'')~/prediction=(method:''genscan'',
version:''1.0'',
score:''114.92'')~/match=(desc:''GPI-ANCHOR TRANSMIDASE
(EC 3.-.-.-)'', species:''SACCHAROMYCES CEREVISIAE
(BAKER'S YEAST>
Length = 326
Score = 445 bits (1133), Expect = e-124
Identities = 168/290 (57%), Positives = 213/290 (72%), Gaps = 1/290 (0%)
Query: 17 IAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSR 76
A S+HTNNWAVL+ SRFWFNYRH ANVL IYRSVKRLGIPDSQIILMIADD ACN+R
Sbjct: 37 AAQRSTHTNNWAVLVDASRFWFNYRHVANVLSIYRSVKRLGIPDSQIILMIADDMACNAR 96
Query: 77 NLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPENTPASKRLLTNERS 136
N PG V++NA++ +++YG+++E+DY+GYEVTVE F+RLLT R T SK+LL++ S
Sbjct: 97 NPRPGQVYNNANQHINVYGDDVEVDYRGYEVTVENFVRLLTGRTQNGTARSKKLLSDAGS 156
Query: 137 NILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKI 196
N+LIY+TGHGGDGF+KFQD+EE++S++LAD I+Q+ + KRYNE+ FMVDTCQA SLY K
Sbjct: 157 NVLIYLTGHGGDGFLKFQDSEEITSQELADGIQQMWEKKRYNELFFMVDTCQAASLYEKF 216
Query: 197 YSPNVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSNLEFLENRVDSKSKLTMQDLINS 256
SPNVLA+ SS VG S SHH D IGV +IDR+T+ LEFLE V SK T+ + +
Sbjct: 217 TSPNVLAVASSLVGEDSLSHHVDPSIGVYMIDRYTYYALEFLEK-VQPFSKRTIGEFLQV 275
Query: 257 YNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIELHSEKINWMLPGE 306
ST GV+ R P + ITDFFG +R + +++IN L E
Sbjct: 276 CPKRVCISTVGVRKDLYPRDPHKVPITDFFGAIRPTRVSTDRINVTLANE 325
>gb|AAA29895.1| (M21308) hemoglobinase [Schistosoma mansoni]
Length = 429
Score = 439 bits (1118), Expect = e-122
Identities = 87/367 (23%), Positives = 159/367 (42%), Gaps = 37/367 (10%)
Query: 1 MIVQFVALLLLNLLQI---------IAAES-SHTNNWAVLISTSRFWFNYRHTANVLGIY 50
M+ + +L++L + ++ E+ S N WAVL++ S + NYRH A+V Y
Sbjct: 2 MLFSLFLISILHILLVKCQLDTNYEVSDETVSDNNKWAVLVAGSNGYPNYRHQADVCHAY 61
Query: 51 RSVKRLGIPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVE 110
++ GI II M+ DD A N N F G +F++ + D Y E + IDY+G +V +
Sbjct: 62 HVLRSKGIKPEHIITMMYDDIAYNLMNPFLGKLFNDYNHK-DWY-EGVVIDYRGKKVNSK 119
Query: 111 AFIRLLTERVPENTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQ 170
F+++L K L + + ++ IY T HG G I F D +EL +++ ++
Sbjct: 120 TFLKVLKGDKSAG---GKVLKSGKNDDVFIYFTDHGAPGLIAFPD-DELYAKEFMSTLKY 175
Query: 171 IHQHKRYNEILFMVDTCQANSLYTKIYSPN--VLAIGSSEVGTSSYSHHA-DIDIGVAVI 227
+H HKRY++++ ++ ++ S++ +I N + A ++ SYS D I +
Sbjct: 176 LHSHKRYSKLVIYIEANESGSMFQQILPSNLSIYATTAANPTECSYSTFCGDPTITTCLA 235
Query: 228 DRFTFSNLEFLENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFG 287
D ++++ + + LT + L Y + + + +++F G
Sbjct: 236 DLYSYNWIVDSQTH-----HLTQRTLDQQYKEVKRETDLSHVQRYGDTRMGKLYVSEFQG 290
Query: 288 NVRDIELHSEKINWMLPGENTTKPSIKRNSFV--FQAQNDMQDDGKGFGISNLKSFLPPT 345
+ EN P R+S + + + KSFL
Sbjct: 291 SRDKS-----------STENDESPMKPRHSIASRDIPLHTLHRQIMMTNNAEDKSFLMQI 339
Query: 346 RELKYKK 352
LK K+
Sbjct: 340 LGLKLKR 346
CPU time: 101.67 user secs. 2.56 sys. secs 104.23 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.312 0.177 0.550
Gapped
Lambda K H
0.270 0.0620 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 264902829
Number of Sequences: 887402
Number of extensions: 15555517
Number of successful extensions: 28066
Number of sequences better than 10.0: 85
Number of HSP's better than 10.0 without gapping: 75
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 27502
Number of HSP's gapped (non-prelim): 92
length of query: 380
length of database: 277,845,442
effective HSP length: 57
effective length of query: 323
effective length of database: 227,263,528
effective search space: 73406119544
effective search space used: 73406119544
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (20.9 bits)
S2: 74 (32.8 bits)
