Sequences with E-value BETTER than threshold (0.002)
emb|CAC18099.1| putative legumain [Zea mays] 608 e-173
dbj|BAC86022.1| unnamed protein product [Homo sapiens] 607 e-172
dbj|BAC54827.1| vacuolar processing enzyme-1a [Nicotiana tabacum] 606 e-172
dbj|BAC54828.1| vacuolar processing enzyme-1b [Nicotiana tabacum] 606 e-172
dbj|BAA76744.1| asparaginyl endopeptidase (VmPE-1) [Vigna mungo] 605 e-172
emb|CAB64544.1| legumain-like protease [Zea mays] 603 e-171
gb|AAF69014.1| cysteine protease [Ipomoea batatas] 602 e-171
dbj|BAC54830.1| vacuolar processing enzyme-3 [Nicotiana tabacum] 602 e-171
emb|CAB64545.1| legumain-like protease [Zea mays] 600 e-170
sp|P49043|VPE_CITSI Vacuolar processing enzyme precursor (VPE) >... 598 e-170
sp|P49042|VPE_RICCO Vacuolar processing enzyme precursor (VPE) >... 598 e-170
sp|P49045|VPE_SOYBN Vacuolar processing enzyme precursor (VPE) >... 598 e-170
gb|AAD04883.1| C13 endopeptidase NP1 precursor [Zea mays] 598 e-169
ref|NP_195020.1| gamma-VPE (vacuolar processing enzyme) [Arabido... 598 e-169
sp|Q39119|VPEG_ARATH Vacuolar processing enzyme, gamma-isozyme p... 598 e-169
ref|NP_180165.1| vacuolar processing enzyme -related [Arabidopsi... 597 e-169
dbj|BAC41386.1| asparaginyl endopeptidase REP-2 [Oryza sativa (j... 596 e-169
pir||T12044 probable legumain (EC 3.4.22.34) precursor - kidney ... 595 e-169
sp|P49044|VPE_VICSA Vacuolar processing enzyme precursor (VPE) (... 594 e-168
pir||T12043 probable legumain (EC 3.4.22.34) precursor - kidney ... 594 e-168
emb|CAC18100.1| putative legumain [Zea mays] 593 e-168
gb|AAF89646.1| seed maturation protein PM40 [Glycine max] 593 e-168
sp|P49046|LEGU_CANEN Legumain precursor (Asparaginyl endopeptida... 592 e-168
gb|AAK15049.1| asparaginyl endopeptidase [Vigna radiata] 591 e-167
ref|NP_918390.1| asparaginyl endopeptidase [Oryza sativa (japoni... 591 e-167
dbj|BAC54829.1| vacuolar processing enzyme-2 [Nicotiana tabacum] 589 e-167
emb|CAB16318.1| cysteine proteinase precursor [Vicia narbonensis] 589 e-167
dbj|BAA76745.1| asparaginyl endopeptidase (VmPE-1A) [Vigna mungo] 588 e-167
ref|NP_176458.1| beta-VPE [Arabidopsis thaliana] >gi|22002012|sp... 586 e-166
pir||T10944 cysteine proteinase (EC 3.4.22.-) precursor - spring... 584 e-165
gb|AAF89679.1| asparaginyl endopeptidase [Sesamum indicum] 578 e-163
emb|CAC43295.1| putative vacuolar processing enzyme [Beta vulgaris] 577 e-163
pir||S60050 vacuolar processing enzyme (EC 3.4.22.-) isozyme bet... 576 e-163
dbj|BAC76418.1| vacuolar processing enzyme [Oryza sativa (japoni... 571 e-161
ref|XP_327545.1| hypothetical protein [Neurospora crassa] >gi|28... 547 e-154
gb|AAL40390.1| C13 cysteine proteinase precursor [Oryza sativa s... 534 e-150
emb|CAB42655.1| putative preprolegumain [Vicia narbonensis] 533 e-150
emb|CAB42651.1| putative preprolegumain [Nicotiana tabacum] 528 e-148
dbj|BAA09530.1| cysteine protease [Homo sapiens] 522 e-147
sp|Q99538|LGMN_HUMAN Legumain precursor (Asparaginyl endopeptida... 521 e-146
ref|NP_005597.2| legumain; protease, cysteine, 1 (legumain) [Hom... 521 e-146
gb|AAH56842.1| MGC64351 protein [Xenopus laevis] 512 e-144
sp|Q9R0J8|LGMN_RAT Legumain precursor (Asparaginyl endopeptidase... 512 e-144
ref|NP_071562.2| protease, cysteine, 1 (legumain) [Rattus norveg... 512 e-143
ref|NP_776526.1| legumain [Bos taurus] >gi|16197488|dbj|BAB69947... 511 e-143
ref|NP_035305.1| legumain; protease, cysteine, 1; preprolegumain... 510 e-143
emb|CAD61872.1| unnamed protein product [Homo sapiens] 510 e-143
emb|CAD61895.1| unnamed protein product [Homo sapiens] 509 e-143
gb|AAD04882.1| C13 endopeptidase NP1 precursor [Hordeum vulgare] 501 e-140
gb|EAA46796.1| hypothetical protein MG10490.4 [Magnaporthe grise... 499 e-140
Alignments
>emb|CAC18099.1| putative legumain [Zea mays]
Length = 486
Score = 608 bits (1553), Expect = e-173
Identities = 79/380 (20%), Positives = 149/380 (38%), Gaps = 32/380 (8%)
Query: 1 MSPYFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLG 60
+ P L + + + WAVL+A S ++NYRH A++ Y+ +K+ G
Sbjct: 25 LEPAIRLPSQRAAA---ADETDDGDVGTRWAVLIAGSSGYYNYRHQADICHAYQIMKKGG 81
Query: 61 IPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-L 119
+ D II+ + DD+A +P N PG + N + D+Y + DY G +V V+NF + L
Sbjct: 82 LKDENIIVFMYDDIAHSPENPRPGVII-NHPQGGDVYA-GVPKDYTGRDVNVDNFFAVLL 139
Query: 120 TDRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKR 179
++ K + S ++ V+ + HGG L + DL D +
Sbjct: 140 GNKTALRGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGT 199
Query: 180 YHELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVI 230
Y L+F ++ C++ +++ N+ AT +S D+SS+ + + +
Sbjct: 200 YKSLVFYLEACESGSIFEGLLPNDINVYATTASNADESSWGTYCPGEFPSPPPEYDTCLG 259
Query: 231 DRWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRL 286
D Y + ++E+ ++L Y K + + G E +
Sbjct: 260 D---LYSVAWMEDSDFHNLRTESLK---QQYKLVKDRTAVHDTFSYGSHVMQYGALELNV 313
Query: 287 RTVVDFFGNVQSVE--IEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAV-ANSTSQQE 343
+ + + G + + D P K + Q F Q Y A+S+ +
Sbjct: 314 QRLFSYIGTDPANDGNTFIEDNSLPSFSK----AVNQRDADLVYFWQKYRKLADSSHAKN 369
Query: 344 ETRSAPAYEVKKRNVGPARL 363
E R + R+ + +
Sbjct: 370 EARKELLEVMAHRSHVDSSV 389
>dbj|BAC86022.1| unnamed protein product [Homo sapiens]
Length = 483
Score = 607 bits (1550), Expect = e-172
Identities = 75/374 (20%), Positives = 143/374 (38%), Gaps = 30/374 (8%)
Query: 8 QALPFLLSLLFAVF--ASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQ 65
LP S F + + WAVL+A S ++NYRH ++V Y+ +++ G+ +
Sbjct: 25 LRLPSEASTFFKAPGGDQNDEGTRWAVLIAGSNGYWNYRHQSDVCHAYQLLRKGGLKEEN 84
Query: 66 IILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFI-RLLTDRLD 124
I++ + DD+A N N PG + N+ D+Y + DY G +VTV NF +L ++
Sbjct: 85 IVVFMYDDIAFNEENPRPGVII-NSPHGNDVY-KGVPKDYIGEDVTVGNFFAAILGNKSA 142
Query: 125 EDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELL 184
K + S ++ +Y + HGG L + A DL + + Y L+
Sbjct: 143 LTGGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTNPYVYASDLIEVLKKKHASGSYKSLV 202
Query: 185 FMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHADN-------DVGVAVIDRWTY 235
F ++ C++ +++ NI AT +S ++SS+ + + + D
Sbjct: 203 FYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEYPSPPSEYETCLGD---L 259
Query: 236 YVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS---NPGVRWDLFPGGEQEGRLRTVVDF 292
Y + ++E+ +T L Y+ K + N + G+ +V +
Sbjct: 260 YSVAWMEDSDIHNLQTET---LHQQYELVKQRTMNGNSIYGSHVMQYGDIGLSENNLVLY 316
Query: 293 FGNVQSVEI--EKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVAN-STSQQEETRSAP 349
G + + K + Q F + A +S++
Sbjct: 317 LGTNPANDNFTFVLKNSLVPPSK----AVNQRDADLIHFWDKFRKAPVGSSRKAAAEKQI 372
Query: 350 AYEVKKRNVGPARL 363
+ R +
Sbjct: 373 LEAMSHRMHIDDSM 386
>dbj|BAC54827.1| vacuolar processing enzyme-1a [Nicotiana tabacum]
Length = 490
Score = 606 bits (1548), Expect = e-172
Identities = 77/380 (20%), Positives = 144/380 (37%), Gaps = 37/380 (9%)
Query: 8 QALPFLLSLLF--------AVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRL 59
LP +S F + WA+L+A S ++NYRH A++ Y+ +K+
Sbjct: 27 LKLPSEVSRFFGADKSNVGDDHDDDSVGTRWAILLAGSNGYWNYRHQADICHAYQLLKKG 86
Query: 60 GIPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFI-RL 118
G+ D I++ + DD+A N N PG + N+ D+Y + DY G +VTV NF L
Sbjct: 87 GLKDENIVVFMYDDIANNEENPRPGVII-NSPHGEDVY-KGVPKDYTGDDVTVNNFFAAL 144
Query: 119 LTDRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKK 178
L ++ K + S ++L++ + HGG L + A DL D +
Sbjct: 145 LGNKTALSGGSGKVVNSGPNDHILIFYSDHGGPGVLGMPTDPYLYANDLIDVLKKKHASG 204
Query: 179 RYHELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHADN-------DVGVAV 229
Y L+F ++ C++ +++ NI AT +S ++SS+ + + +
Sbjct: 205 TYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEYPSPPIEYETCL 264
Query: 230 IDRWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHSNPG---VRWDLFPGGEQEGRL 286
D Y + ++E+ ++L Y + + G + G+
Sbjct: 265 GD---LYSISWMEDSELHNLRTESLK---QQYHLVRERTATGNPVYGSHVMQYGDLHLSK 318
Query: 287 RTVVDFFGNVQSVEI--EKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTS-QQE 343
+ + G + + D S + Q F + A S ++
Sbjct: 319 DALYLYMGTNPANDNYTFMDDNSLRVS-----KAVNQRDADLLHFWYKFRKAPEGSVRKI 373
Query: 344 ETRSAPAYEVKKRNVGPARL 363
E + + R +
Sbjct: 374 EAQKQLNEAISHRVHLDNSI 393
>dbj|BAC54828.1| vacuolar processing enzyme-1b [Nicotiana tabacum]
Length = 489
Score = 606 bits (1547), Expect = e-172
Identities = 75/379 (19%), Positives = 143/379 (36%), Gaps = 36/379 (9%)
Query: 8 QALPFLLSLLFAVF-------ASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLG 60
LP +S F + WA+L+A S ++NYRH A++ Y+ +K+ G
Sbjct: 27 LKLPSEVSRFFGADESNAGDHDDDSVGTRWAILLAGSNGYWNYRHQADICHAYQLLKKGG 86
Query: 61 IPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-L 119
+ D I++ + DD+A N N G + N+ D+Y + DY G +VTV+NF + L
Sbjct: 87 LKDENIVVFMYDDIANNEENPRRGVII-NSPHGEDVY-KGVPKDYTGDDVTVDNFFAVIL 144
Query: 120 TDRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKR 179
++ K + S ++ ++ + HGG L + A DL D +
Sbjct: 145 GNKTALSGGSGKVVNSGPNDHIFIFYSDHGGPGVLGMPTDPYLYANDLIDVLKKKHASGT 204
Query: 180 YHELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHADNDVG-------VAVI 230
Y L+F ++ C++ +++ NI AT +S ++SS+ + + +
Sbjct: 205 YKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEYPSPPIEYMTCLG 264
Query: 231 DRWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHSNPG---VRWDLFPGGEQEGRLR 287
D Y + ++E+ ++L Y K + G + G+
Sbjct: 265 D---LYSISWMEDSELHNLRTESLK---QQYHLVKERTATGNPVYGSHVMQYGDLHLSKD 318
Query: 288 TVVDFFGNVQSVEI--EKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTS-QQEE 344
+ + G + + D S + Q F + A S ++ E
Sbjct: 319 ALYLYMGTNPANDNYTFMDDNSLRVS-----KAVNQRDADLLHFWHKFRTAPEGSVRKIE 373
Query: 345 TRSAPAYEVKKRNVGPARL 363
+ + R +
Sbjct: 374 AQKQLNEAISHRVHLDNSV 392
>dbj|BAA76744.1| asparaginyl endopeptidase (VmPE-1) [Vigna mungo]
Length = 483
Score = 605 bits (1544), Expect = e-172
Identities = 73/375 (19%), Positives = 143/375 (37%), Gaps = 30/375 (8%)
Query: 8 QALPFLLSLLFAVF--ASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQ 65
+P S F + + WAVL+A S ++NYRH ++V Y+ + + G+ +
Sbjct: 25 LRMPSEASRFFQAPATDENDEGTRWAVLIAGSNGYWNYRHQSDVCHAYQLLTKGGLKEEN 84
Query: 66 IILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFI-RLLTDRLD 124
I++ + DD+A N N PG + N+ D+Y + DY G +VTV NF +L ++
Sbjct: 85 IVVFMYDDIAFNEENPRPGVII-NSPHGNDVY-KGVPKDYVGEDVTVNNFFAAILGNKSA 142
Query: 125 EDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELL 184
K + S ++ +Y + HGG L + + A DL + + Y L
Sbjct: 143 LTGGSGKVVNSGPNDHIFIYYSDHGGPGVLGMPTSPYMYASDLIEVLKKKHASGTYKSLA 202
Query: 185 FMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVIDRWTY 235
F ++ C++ +++ NI AT ++ ++SS+ + + + D
Sbjct: 203 FYLEGCESGSIFGGLLPEGLNIYATTAANAEESSWGTYCPGDNPSPPPEYETCLGD---L 259
Query: 236 YVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS---NPGVRWDLFPGGEQEGRLRTVVDF 292
Y + ++E+ +T L ++ K + N + G+ V +
Sbjct: 260 YSVAWMEDSDIHNLRTET---LHQQFELVKQRTMNGNSAYGSHVMQYGDVGLSKNNVSLY 316
Query: 293 FGNVQSVEIEK--ADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVAN-STSQQEETRSAP 349
G + + K + Q F + A +S++ +
Sbjct: 317 LGTNPANDNFPFREKNSLVPPSK----AVNQRDADLVHFWDKFPKAPLGSSRKSVAQKQI 372
Query: 350 AYEVKKRNVGPARLT 364
+ R +T
Sbjct: 373 LEAMSHRMHIDDSVT 387
>emb|CAB64544.1| legumain-like protease [Zea mays]
Length = 486
Score = 603 bits (1540), Expect = e-171
Identities = 77/380 (20%), Positives = 146/380 (38%), Gaps = 32/380 (8%)
Query: 1 MSPYFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLG 60
+ L + + WAVL+A S ++NYRH A++ Y+ +K+ G
Sbjct: 25 LETAIRLPSQRAAA---ADETDDGAVGTRWAVLIAGSSGYYNYRHQADICHAYQIMKKGG 81
Query: 61 IPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-L 119
+ D II+ + DD+A +P N PG + N + D+Y + DY G +V V+NF + L
Sbjct: 82 LKDENIIVFMYDDIAHSPENPRPGVII-NHPQGGDVYA-GVPKDYTGRDVNVDNFFAVLL 139
Query: 120 TDRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKR 179
++ K + S ++ V+ + HGG L + DL D +
Sbjct: 140 GNKTALRGGSGKVVDSGPDDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGT 199
Query: 180 YHELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVI 230
Y L+F ++ C++ +++ N+ AT +S ++SS+ + + +
Sbjct: 200 YKSLVFYLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLG 259
Query: 231 DRWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRL 286
D Y + ++E+ ++L Y K + + G E +
Sbjct: 260 D---LYSVAWMEDSDFHNLRTESLK---QQYKLVKDRTAVHDTFSYGSHVMQYGALELNV 313
Query: 287 RTVVDFFGNVQSVE--IEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAV-ANSTSQQE 343
+ + + G + + D P K + Q F Q Y A+S +
Sbjct: 314 QHLFSYIGTDPANDGNTFIEDNSLPSFSK----AVNQRDADLVYFWQKYRKFADSPPAKS 369
Query: 344 ETRSAPAYEVKKRNVGPARL 363
E R + R+ + +
Sbjct: 370 EARKELLEVMAHRSHVDSSV 389
>gb|AAF69014.1| cysteine protease [Ipomoea batatas]
Length = 492
Score = 602 bits (1537), Expect = e-171
Identities = 75/378 (19%), Positives = 142/378 (36%), Gaps = 30/378 (7%)
Query: 8 QALPFLLSLLFAVFA--------SAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRL 59
LP F + WAVL+A S ++NYRH A++ Y+ +K
Sbjct: 26 LKLPSEARRFFRPAEEENREADGDDSVGTRWAVLIAGSNGYWNYRHQADICHAYQILKAG 85
Query: 60 GIPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL- 118
G+ D I++ + DD+A N N G + N+ D+Y + DY G +VT N + +
Sbjct: 86 GLKDENIVVFMYDDIAYNEENPRKGIII-NSPHGEDVYH-GVPKDYTGDDVTANNLLAVI 143
Query: 119 LTDRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKK 178
L D+ K + S ++ +Y + HGG L + + A +L A +
Sbjct: 144 LGDKSAVKGGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPYLYADELNAALKKKHAAG 203
Query: 179 RYHELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAV 229
Y L+F ++ C++ +++ NI AT +S +SS+ + + +
Sbjct: 204 AYKSLVFYLEACESGSIFEGILPKDINIYATTASNAIESSWGTYCPGEYPSPPPEYETCL 263
Query: 230 IDRWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS---NPGVRWDLFPGGEQEGRL 286
D Y + ++E+ ++L Y+ K + N + G+ E
Sbjct: 264 GD---LYSIAWMEDSDIHNLRTESLK---QQYNLVKDRTLNGNTAYGSHVMQYGDLELNA 317
Query: 287 RTVVDFFGNVQSVEIEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTS-QQEET 345
++ + G + E ++ L + Q F + A S ++ E
Sbjct: 318 DSLFMYMGTNPANENFTFVDEKSLKLSAPRRAVNQRDADLLHFWDKFRNAPEGSARKSEA 377
Query: 346 RSAPAYEVKKRNVGPARL 363
+ + R +
Sbjct: 378 QKQFTEAITHRTHLDNSI 395
>dbj|BAC54830.1| vacuolar processing enzyme-3 [Nicotiana tabacum]
Length = 481
Score = 602 bits (1536), Expect = e-171
Identities = 78/372 (20%), Positives = 144/372 (37%), Gaps = 29/372 (7%)
Query: 8 QALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQII 67
LP S F + WAVL+A S ++NYRH A+V Y+ +++ G+ D II
Sbjct: 26 LKLPSEASRFFDEA-DDSVGTRWAVLLAGSNGYWNYRHQADVCHAYQLLRKGGLKDENII 84
Query: 68 LMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LTDRLDED 126
+ + DD+A N N G + N+ D+Y + DY G +V V+NF+ + L ++
Sbjct: 85 MFMYDDIAYNEENPRQGVII-NSPAGEDVY-KGVPKDYTGDDVNVDNFLAVLLGNKTALT 142
Query: 127 VPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELLFM 186
K + S ++ ++ + HGG L + A DL D + Y L+
Sbjct: 143 GGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTNPYLYASDLIDVLKKKHASGTYKSLVLY 202
Query: 187 IDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHADND-------VGVAVIDRWTYYV 237
I+ C++ +++ NI AT +S +SS+ + D + D Y
Sbjct: 203 IEACESGSIFEGLLPKGLNIYATTASNAVESSWGTYCPGDYPSLPPGYETCLGD---LYA 259
Query: 238 LEFLENRVTSLDSKQTLGDLFDSYDETKIHS---NPGVRWDLFPGGEQEGRLRTVVDFFG 294
+ ++E+ + +L Y K + N + G+ + + ++ + G
Sbjct: 260 VSWMEDSEMHNLRTE---NLRQQYHLVKERTANGNSAYGSHVLQFGDLQLGMDSLFMYMG 316
Query: 295 NVQSVEI--EKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTS-QQEETRSAPAY 351
+ + D S K + Q F + A S ++ E +
Sbjct: 317 TNPANDNYTYVDDNSLRASSK----AVNQRDADLLHFWDKFRKAPEGSARKVEAQKQFTE 372
Query: 352 EVKKRNVGPARL 363
+ R +
Sbjct: 373 AMSHRMHLDNSM 384
>emb|CAB64545.1| legumain-like protease [Zea mays]
Length = 481
Score = 600 bits (1532), Expect = e-170
Identities = 76/380 (20%), Positives = 149/380 (39%), Gaps = 37/380 (9%)
Query: 1 MSPYFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLG 60
+ P L + + WAVL+A S ++NYRH A++ Y+ +K+ G
Sbjct: 25 LEPTIRLPSDRAAA--------DDAVGTRWAVLIAGSNGYYNYRHQADICHAYQIMKKGG 76
Query: 61 IPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-L 119
+ D I++ + DD+A +P N PG + N + D+Y + DY G +V V+NF + L
Sbjct: 77 LKDENIVVFMYDDIAHSPENPRPGVII-NHPQGGDVYA-GVPKDYTGRDVNVDNFFAVLL 134
Query: 120 TDRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKR 179
++ K + S ++ V+ + HGG L + DL D +
Sbjct: 135 GNKTALRGGSGKVVDSGPNDHISVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGT 194
Query: 180 YHELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVI 230
Y L+F ++ C++ +++ N+ AT +S ++SS+ + + +
Sbjct: 195 YKSLVFYLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLG 254
Query: 231 DRWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRL 286
D Y + ++E+ ++L Y+ K + + G +
Sbjct: 255 D---LYSVAWMEDSDFHNLRTESLK---QQYNLVKDRTAVQDTFSYGSHVMQYGSLGLNV 308
Query: 287 RTVVDFFGNVQSVE--IEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAV-ANSTSQQE 343
+ + + G + + D P K + Q F Q Y A+S+ ++
Sbjct: 309 KHLFSYIGTNPANDDNTFIEDNSLPSFSK----AVNQRDADLVYFWQKYRKLADSSPEKN 364
Query: 344 ETRSAPAYEVKKRNVGPARL 363
E R + R+ + +
Sbjct: 365 EARRELLEVMAHRSHVDSSV 384
>sp|P49043|VPE_CITSI Vacuolar processing enzyme precursor (VPE)
pir||S51117 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - sweet
orange
emb|CAA87720.1| cystein proteinase (by similarity) [Citrus sinensis]
prf||2208463A vascular processing protease
Length = 494
Score = 598 bits (1527), Expect = e-170
Identities = 75/379 (19%), Positives = 141/379 (36%), Gaps = 35/379 (9%)
Query: 8 QALPFLLSLLF--------AVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRL 59
LP F + WAVL+A S ++NYRH A++ Y+ +++
Sbjct: 31 LKLPSEAYRFFHNGGGGAKVNDDDDSVGTRWAVLLAGSNGFWNYRHQADICHAYQLLRKG 90
Query: 60 GIPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIR-L 118
G+ D II+ + DD+A N N PG + N D+Y + DY G +VTVE F +
Sbjct: 91 GLKDENIIVFMYDDIAFNEENPRPGVII-NHPHGDDVY-KGVPKDYTGEDVTVEKFFAVV 148
Query: 119 LTDRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKK 178
L ++ K + S ++ ++ + HGG L + I A +L D +
Sbjct: 149 LGNKTALTGGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSRYIYADELIDVLKKKHASG 208
Query: 179 RYHELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAV 229
Y L+F ++ C++ +++ NI AT +S ++SS+ + + +
Sbjct: 209 NYKSLVFYLEACESGSIFEGLLLEGLNIYATTASNAEESSWGTYCPGEIPGPPPEYSTCL 268
Query: 230 IDRWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHSNP--GVRWDLFPGGEQEGRLR 287
D Y + ++E+ +T L Y+ K + + G+
Sbjct: 269 GD---LYSIAWMEDSDIHNLRTET---LHQQYELVKTRTASYNSYGSHVMQYGDIGLSKN 322
Query: 288 TVVDFFGNVQSVEI--EKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANST-SQQEE 344
+ + G + + + + K + Q F Y A ++ E
Sbjct: 323 NLFTYLGTNPANDNYTFVDENSLRPASK----AVNQRDADLLHFWDKYRKAPEGTPRKAE 378
Query: 345 TRSAPAYEVKKRNVGPARL 363
+ + R +
Sbjct: 379 AQKQFFEAMSHRMHVDHSI 397
>sp|P49042|VPE_RICCO Vacuolar processing enzyme precursor (VPE)
pir||JQ2387 vacuolar processing enzyme (EC 3.4.22.-) precursor - castor bean
dbj|BAA04225.1| precursor of vacuolar processing enzyme [Ricinus communis]
Length = 497
Score = 598 bits (1527), Expect = e-170
Identities = 72/377 (19%), Positives = 143/377 (37%), Gaps = 29/377 (7%)
Query: 2 SPYFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGI 61
P + V + + WAVLVA S + NYRH A+V Y+ +++ G+
Sbjct: 38 EPGILMPTEEAEP---VQVDDDDQLGTRWAVLVAGSMGFGNYRHQADVCHAYQLLRKGGL 94
Query: 62 PDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LT 120
+ II+ + DD+A N N PG + N + D+Y + DY G VT +N + L
Sbjct: 95 KEENIIVFMYDDIAKNELNPRPGVII-NHPQGEDVYA-GVPKDYTGEHVTAKNLYAVLLG 152
Query: 121 DRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRY 180
D+ K + S + +Y + HGG L + + A D + + Y
Sbjct: 153 DKSAVQGGSGKVVDSKPNDRIFLYYSDHGGPGVLGMPNLPYLYAMDFIEVLKKKHAAGGY 212
Query: 181 HELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVID 231
+++ ++ C++ +++ +I T +S +SS+ + + + D
Sbjct: 213 KKMVIYVEACESGSIFEGIMPKDVDIYVTTASNAQESSWGTYCPGMEPSPPPEFTTCLGD 272
Query: 232 RWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRLR 287
Y + ++E+ + K+T+ Y K + + G Q +
Sbjct: 273 ---LYSVAWMEDSESHNLKKETVK---QQYSSVKARTSNYNTYAAGSHVMQYGNQSIKAD 326
Query: 288 TVVDFFGNVQSVEIEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVA-NSTSQQEETR 346
+ F G + + L + + Q + Q Y + N + +++E
Sbjct: 327 KLYLFQGFDPASVNFPPNN---AHLNAPMEVVNQRDAELHFMWQLYKRSENGSEKKKEIL 383
Query: 347 SAPAYEVKKRNVGPARL 363
+K R+ + +
Sbjct: 384 QQIKDAIKHRSHLDSSM 400
>sp|P49045|VPE_SOYBN Vacuolar processing enzyme precursor (VPE)
pir||T07132 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - soybean
dbj|BAA06030.1| cysteine proteinase [Glycine max]
Length = 495
Score = 598 bits (1527), Expect = e-170
Identities = 76/375 (20%), Positives = 139/375 (36%), Gaps = 33/375 (8%)
Query: 4 YFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPD 63
L P S E + WAVLVA S + NYRH A+V Y+ + + G+ +
Sbjct: 42 VIKLPTEP-------VDADSDEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGLKE 94
Query: 64 SQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LTDR 122
I++ + DD+A N N G + N DLY + DY G VT EN + L D+
Sbjct: 95 ENIVVFMYDDIATNELNPRHGVII-NHPEGEDLYA-GVPKDYTGDNVTTENLFAVILGDK 152
Query: 123 LDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHE 182
K + S + +Y + HGG L + + A D D + Y E
Sbjct: 153 SKLKGGSGKVINSKPEDRIFIYYSDHGGPGILGMPNMPYLYAMDFIDVLKKKHASGSYKE 212
Query: 183 LLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVIDRW 233
++ ++ C++ +++ NI T +S ++S+ + + + D
Sbjct: 213 MVIYVEACESGSVFEGIMPKDLNIYVTTASNAQENSWGTYCPGMDPSPPPEYITCLGD-- 270
Query: 234 TYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRLRTV 289
Y + ++E+ ++++ Y K + N + + G+ +
Sbjct: 271 -LYSVAWMEDSEAHNLKRESVK---QQYKSVKQRTSNFNNYAMGSHVMQYGDTNITAEKL 326
Query: 290 VDFFGNVQSVEIEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQ-QEETRSA 348
+ G + G L+ + + Q + Q Y +N S+ + +
Sbjct: 327 YLYQGFDPATVNFPPQN---GRLETKMEVVNQRDAELFLLWQMYQRSNHQSENKTDILKQ 383
Query: 349 PAYEVKKRNVGPARL 363
A VK R +
Sbjct: 384 IAETVKHRKHIDGSV 398
>gb|AAD04883.1| C13 endopeptidase NP1 precursor [Zea mays]
Length = 485
Score = 598 bits (1527), Expect = e-169
Identities = 77/380 (20%), Positives = 150/380 (39%), Gaps = 33/380 (8%)
Query: 1 MSPYFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLG 60
+ P L + + WAVL+A S ++NYRH A++ Y+ +K+ G
Sbjct: 25 LEPTIRLPSER----AAADETDDDAVGTRWAVLIAGSNGYYNYRHQADICHAYQIMKKGG 80
Query: 61 IPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-L 119
+ D I++ + DD+A +P N PG + N + D+Y + DY G +V V+NF + L
Sbjct: 81 LKDENIVVFMYDDIAHSPENPRPGVII-NHPQGGDVYA-GVPKDYTGRDVNVDNFFAVLL 138
Query: 120 TDRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKR 179
++ K + S ++ V+ + HGG L + DL D +
Sbjct: 139 GNKTALRGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGT 198
Query: 180 YHELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVI 230
Y L+F ++ C++ +++ N+ AT +S ++SS+ + + +
Sbjct: 199 YKSLVFYLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLG 258
Query: 231 DRWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRL 286
D Y + ++E+ ++L Y+ K + + G E +
Sbjct: 259 D---LYSVAWMEDSDFHNLRTESLK---QQYNLVKDRTAVQDTFSYGSHVMQYGSLELNV 312
Query: 287 RTVVDFFGNVQSVE--IEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAV-ANSTSQQE 343
+ + + G + + D P K + Q F Q Y A+S+ ++
Sbjct: 313 KHLFSYIGTNPANDDNTFIEDNSLPSLSK----AVNQRDADLVYFWQKYRKLADSSPEKN 368
Query: 344 ETRSAPAYEVKKRNVGPARL 363
E R + R+ + +
Sbjct: 369 EARRELLEVMAHRSHVDSSV 388
>ref|NP_195020.1| gamma-VPE (vacuolar processing enzyme) [Arabidopsis thaliana]
pir||T05302 vacuolar processing enzyme (EC 3.4.22.-) isozyme gamma precursor -
Arabidopsis thaliana
emb|CAA21203.1| gamma-VPE (vacuolar processing enzyme) [Arabidopsis thaliana]
emb|CAB80011.1| gamma-VPE (vacuolar processing enzyme) [Arabidopsis thaliana]
gb|AAK43975.1| putative vacuolar processing enzyme gamma-VPE [Arabidopsis
thaliana]
gb|AAL11612.1| AT4g32940/F26P21_60 [Arabidopsis thaliana]
gb|AAL15210.1| putative vacuolar processing enzyme gamma-VPE [Arabidopsis
thaliana]
gb|AAM91361.1| At4g32940/F26P21_60 [Arabidopsis thaliana]
Length = 494
Score = 598 bits (1526), Expect = e-169
Identities = 75/374 (20%), Positives = 144/374 (38%), Gaps = 31/374 (8%)
Query: 9 ALPFLLSLLFAVFASAEH---TSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQ 65
LP S F + + + WAVLVA S ++NYRH A++ Y+ +++ G+ +
Sbjct: 36 KLPSQASRFFRPAENDDDSNSGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEEN 95
Query: 66 IILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LTDRLD 124
I++ + DD+A N N PGT+ N+ D+Y + DY G +V V+N + L D+
Sbjct: 96 IVVFMYDDIANNYENPRPGTII-NSPHGKDVY-QGVPKDYTGDDVNVDNLFAVILGDKTA 153
Query: 125 EDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELL 184
K + S ++ ++ + HGG L + + A DL D + Y L+
Sbjct: 154 VKGGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLV 213
Query: 185 FMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVIDRWTY 235
F ++ C++ +++ NI AT +S ++SS+ + + + D
Sbjct: 214 FYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEEPSPPPEYETCLGD---L 270
Query: 236 YVLEFLENRVTSLDSKQTLGDLFDSYDETKIHSN---PGVRWDLFPGGEQEGRLRTVVDF 292
Y + ++E+ +T L Y+ K + + G+ + +
Sbjct: 271 YSVAWMEDSGMHNLQTET---LHQQYELVKRRTAPVGYSYGSHVMQYGDVGISKDNLDLY 327
Query: 293 FGNVQSVEIEK--ADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTS-QQEETRSAP 349
G + + + Q F + Y A S ++ E +
Sbjct: 328 MGTNPANDNFTFADANSLKPPSRV----TNQRDADLVHFWEKYRKAPEGSARKTEAQKQV 383
Query: 350 AYEVKKRNVGPARL 363
+ R +
Sbjct: 384 LEAMSHRLHIDNSV 397
>sp|Q39119|VPEG_ARATH Vacuolar processing enzyme, gamma-isozyme precursor (Gamma-VPE)
dbj|BAA18924.1| gamma-VPE [Arabidopsis thaliana]
Length = 490
Score = 598 bits (1526), Expect = e-169
Identities = 75/374 (20%), Positives = 144/374 (38%), Gaps = 31/374 (8%)
Query: 9 ALPFLLSLLFAVFASAEH---TSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQ 65
LP S F + + + WAVLVA S ++NYRH A++ Y+ +++ G+ +
Sbjct: 32 KLPSQASRFFRPAENDDDSNSGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEEN 91
Query: 66 IILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LTDRLD 124
I++ + DD+A N N PGT+ N+ D+Y + DY G +V V+N + L D+
Sbjct: 92 IVVFMYDDIANNYENPRPGTII-NSPHGKDVY-QGVPKDYTGDDVNVDNLFAVILGDKTA 149
Query: 125 EDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELL 184
K + S ++ ++ + HGG L + + A DL D + Y L+
Sbjct: 150 VKGGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLV 209
Query: 185 FMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVIDRWTY 235
F ++ C++ +++ NI AT +S ++SS+ + + + D
Sbjct: 210 FYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEEPSPPPEYETCLGD---L 266
Query: 236 YVLEFLENRVTSLDSKQTLGDLFDSYDETKIHSN---PGVRWDLFPGGEQEGRLRTVVDF 292
Y + ++E+ +T L Y+ K + + G+ + +
Sbjct: 267 YSVAWMEDSGMHNLQTET---LHQQYELVKRRTAPVGYSYGSHVMQYGDVGISKDNLDLY 323
Query: 293 FGNVQSVEIEK--ADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTS-QQEETRSAP 349
G + + + Q F + Y A S ++ E +
Sbjct: 324 MGTNPANDNFTFADANSLKPPSRV----TNQRDADLVHFWEKYRKAPEGSARKTEAQKQV 379
Query: 350 AYEVKKRNVGPARL 363
+ R +
Sbjct: 380 LEAMSHRLHIDNSV 393
>ref|NP_180165.1| vacuolar processing enzyme -related [Arabidopsis thaliana]
sp|P49047|VPEA_ARATH Vacuolar processing enzyme, alpha-isozyme precursor (Alpha-VPE)
pir||T02629 vacuolar processing enzyme (EC 3.4.22.-) isozyme alpha precursor -
Arabidopsis thaliana
gb|AAC31241.1| putative vacuolar processing enzyme [Arabidopsis thaliana]
dbj|BAA09614.2| alpha-VPE [Arabidopsis thaliana]
gb|AAL24163.1| At2g25940/F17H15.3 [Arabidopsis thaliana]
gb|AAL90957.1| At2g25940/F17H15.3 [Arabidopsis thaliana]
gb|AAM15043.1| putative vacuolar processing enzyme [Arabidopsis thaliana]
Length = 478
Score = 597 bits (1525), Expect = e-169
Identities = 81/370 (21%), Positives = 149/370 (39%), Gaps = 28/370 (7%)
Query: 9 ALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQIIL 68
LP L S F + + ++ WAVLVA S ++NYRH A+V Y+ +K+ G+ + I++
Sbjct: 24 KLPSLASKFFRPTENDDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVV 83
Query: 69 MLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LTDRLDEDV 127
+ DD+A N N PG + N+ D+Y + + DY G EV V+N + + L ++
Sbjct: 84 FMYDDIAKNEENPRPGVII-NSPNGEDVY-NGVPKDYTGDEVNVDNLLAVILGNKTALKG 141
Query: 128 PRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELLFMI 187
K + S ++ +Y + HGG L + + A DL D + + Y L+F +
Sbjct: 142 GSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYL 201
Query: 188 DTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHADN-------DVGVAVIDRWTYYVL 238
+ C++ +++ NI AT +S ++SS+ + + + D Y +
Sbjct: 202 EACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGD---LYSV 258
Query: 239 EFLENRVTSLDSKQTLGDLFDSYDETKIHSN---PGVRWDLFPGGEQEGRLRTVVDFFGN 295
++E+ +T L + Y+ K + + G+ +V F G
Sbjct: 259 AWIEDSEKHNLQTET---LHEQYELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGT 315
Query: 296 VQSVEI--EKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTS-QQEETRSAPAYE 352
+ E + + Q F Y A S ++ E +
Sbjct: 316 NPADENFTFVNENSIRPPSRV----TNQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEA 371
Query: 353 VKKRNVGPAR 362
+ R
Sbjct: 372 MSHRLHVDNS 381
>dbj|BAC41386.1| asparaginyl endopeptidase REP-2 [Oryza sativa (japonica
cultivar-group)]
dbj|BAC41387.1| asparaginyl endopeptidase REP-2 [Oryza sativa (japonica
cultivar-group)]
Length = 496
Score = 596 bits (1523), Expect = e-169
Identities = 69/385 (17%), Positives = 149/385 (37%), Gaps = 35/385 (9%)
Query: 2 SPYFGLQ--------ALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLY 53
P + A S + WAVLVA S + NYRH A+V Y
Sbjct: 27 EPVIRMPGEVVEEEVATVPRGSEGTEEEEKDGVGTRWAVLVAGSSGYGNYRHQADVCHAY 86
Query: 54 RTVKRLGIPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVE 113
+ +++ G+ + I++ + DD+A N N PG + N + D+Y + DY G EVT +
Sbjct: 87 QILRKGGLKEENIVVFMYDDIANNILNPRPGVIV-NHPQGEDVYA-GVPKDYTGDEVTAK 144
Query: 114 NFIRL-LTDRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFG 172
NF + L ++ K + S ++ ++ + HGG L + + A D
Sbjct: 145 NFYAVLLGNKTAVTGGSRKVIDSKPNDHIFIFYSDHGGPGVLGMPNLPYLYAADFMKVLQ 204
Query: 173 QMWEKKRYHELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHADN------- 223
+ Y +++ ++ C++ +++ NI T +S ++SS+ +
Sbjct: 205 EKHASNTYAKMVIYVEACESGSIFEGLMPEDLNIYVTTASNAEESSWGTYCPGMEPSPPS 264
Query: 224 DVGVAVIDRWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPG 279
+ + D Y + ++E+ T ++++ Y+ K + + G +
Sbjct: 265 EYITCLGD---LYSVSWMEDSETHNLKEESIK---KQYEVVKKRTSDMNSYGAGSHVMEY 318
Query: 280 GEQEGRLRTVVDFFGNVQSVEIEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVA-NS 338
G++ + + + G + A+ S + + Q + Y + +
Sbjct: 319 GDRTFKDDKLYLYQGFDPAN----AEVKNKLSWEGPKAAVNQRDADLLFLWRRYELLHDK 374
Query: 339 TSQQEETRSAPAYEVKKRNVGPARL 363
+ ++ + + V R + + +
Sbjct: 375 SEEKLKALREISDTVMHRKLLDSSV 399
>pir||T12044 probable legumain (EC 3.4.22.34) precursor - kidney bean
emb|CAB17079.1| legumain-like proteinase precursor [Phaseolus vulgaris]
Length = 493
Score = 595 bits (1520), Expect = e-169
Identities = 74/375 (19%), Positives = 140/375 (36%), Gaps = 33/375 (8%)
Query: 4 YFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPD 63
L S E + WAVLVA S + NYRH A+V Y+ + + G+ +
Sbjct: 40 VIKLPTQE-------VDAESDEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKE 92
Query: 64 SQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LTDR 122
I++ + DD+A + N PG + +N + D+Y + DY G VT NF + L D+
Sbjct: 93 ENIVVFMYDDIATHELNPRPGVIINN-PQGPDVYA-GVPKDYTGESVTSHNFFAVLLGDK 150
Query: 123 LDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHE 182
K + S + VY + HGG L + + A D D + Y E
Sbjct: 151 SKVKGGSGKVINSKPEDRIFVYYSDHGGPGVLGMPNMPYLYAMDFIDVLKKKHASGGYKE 210
Query: 183 LLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHADN-------DVGVAVIDRW 233
++ ++ C++ +++ NI T +S ++S+ + + + D
Sbjct: 211 MVIYVEACESGSIFEGIMPKDLNIYVTTASNAQENSWGTYCPGMYPPPPPEYITCLGD-- 268
Query: 234 TYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRLRTV 289
Y + ++E+ + K+++ Y K + + + G+ +
Sbjct: 269 -LYSVAWMEDSESHNLKKESVE---QQYQSVKQRTSNFEAYAMGSHVMQYGDANMTAEKL 324
Query: 290 VDFFGNVQSVEIEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQE-ETRSA 348
+ G + G LK + + Q + Q Y +N +++ +
Sbjct: 325 YLYHGFDPATVNFPPHN---GRLKSKMEVVNQRDAELLFMWQVYQRSNHLPEKKTDILKQ 381
Query: 349 PAYEVKKRNVGPARL 363
VK R +
Sbjct: 382 IEEIVKHRKHLDGSV 396
>sp|P49044|VPE_VICSA Vacuolar processing enzyme precursor (VPE) (Proteinase B)
pir||S49175 legumain (EC 3.4.22.34) precursor [similarity] - spring vetch
emb|CAA84383.1| cysteine proteinase [Vicia sativa]
Length = 493
Score = 594 bits (1516), Expect = e-168
Identities = 76/372 (20%), Positives = 143/372 (38%), Gaps = 24/372 (6%)
Query: 8 QALPFLLSLLFAVFASAEH--TSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQ 65
LP S F + + + WA+L+A S ++NYRH ++V Y+ +++ G +
Sbjct: 33 LRLPSETSRFFREPKNDDDFEGTRWAILLAGSNGYWNYRHQSDVCHAYQLLRKGGSKEEN 92
Query: 66 IILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFI-RLLTDRLD 124
II+ + DD+A N N PG + N D+Y + DY G EV +NF LL ++
Sbjct: 93 IIVFMYDDIASNEENPRPGVII-NKPDGDDVYA-GVPKDYTGAEVHADNFYAALLGNKSA 150
Query: 125 EDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELL 184
K + S ++ VY T HGG L + A DL + + Y L+
Sbjct: 151 LTGGSGKVVDSGPNDHIFVYYTDHGGPGVLGMPVGPYLYASDLNEVLKKKHASGTYKSLV 210
Query: 185 FMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHADN-------DVGVAVIDRWTY 235
F ++ C++ +++ NI AT +S ++SS+ ++ + + D
Sbjct: 211 FYLEACESGSIFEGLLPDDLNIYATTASNAEESSWGYYCPGDKPPPPPEYSTCLGD---L 267
Query: 236 YVLEFLENRVTSLDSKQTLGDLFDSYDETKIHSNP-GVRWDLFPGGEQEGRLRTVVDFFG 294
Y + ++E+ ++ L Y K + + G+ + + G
Sbjct: 268 YSIAWMEDSEVHNLQTES---LQQQYKLVKNRTISEPYGSHVMEYGDIGLSKNDLYQYLG 324
Query: 295 NVQSVEI--EKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANST-SQQEETRSAPAY 351
+ + + + L+ + Q F + + A SQ+ E
Sbjct: 325 TNPANDNNSFVDETENSLKLRTPSAAVNQRDADLIHFWEKFRKAPEGSSQKNEAEKQVLE 384
Query: 352 EVKKRNVGPARL 363
+ R +
Sbjct: 385 AMSHRKHIDNSV 396
>pir||T12043 probable legumain (EC 3.4.22.34) precursor - kidney bean
emb|CAB17078.1| asparagine-specific endopeptidase precursor [Phaseolus vulgaris]
Length = 484
Score = 594 bits (1516), Expect = e-168
Identities = 76/376 (20%), Positives = 140/376 (37%), Gaps = 36/376 (9%)
Query: 4 YFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPD 63
+ L + H + WA+L A S ++NYRH A++ Y+ +++ G+ D
Sbjct: 32 FLRLPSDSGNG--------DNVHGTRWAILFAGSSGYWNYRHQADICHAYQLLRKGGLKD 83
Query: 64 SQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFI-RLLTDR 122
II+ + DD+A N N G + N+ ++Y + DY G +VT NF LL D+
Sbjct: 84 ENIIVFMYDDIAFNSENPRRGVII-NSPNGDEVY-KGVPKDYTGEDVTAHNFYAALLGDK 141
Query: 123 LDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHE 182
K + S ++ ++ + HGG L I A DL + + Y
Sbjct: 142 SKLTGGSGKVVNSGPNDHIFIFYSDHGGPGVLGSPAGPYIYASDLNEVLKKKHASGTYKN 201
Query: 183 LLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVIDRW 233
L+F ++ C++ +++ N+ AT +S D+SS+ + + + D
Sbjct: 202 LVFYLEACESGSIFEGLLPEDINVYATTASNADESSWGTYCPGEDPSPPPEYSTCLGD-- 259
Query: 234 TYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHSNPG---VRWDLFPGGEQEGRLRTVV 290
Y + ++E+ +T L Y K + G + G+ +
Sbjct: 260 -LYSVAWMEDSDRHNLRTET---LHQQYKLVKERTISGGLYYGSHVMQYGDVGLSKDILF 315
Query: 291 DFFGNVQSVEI--EKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTS-QQEETRS 347
+ G + E + S K + Q F + A S ++ E R
Sbjct: 316 HYLGTDPANENLTFVDENSLWSSSK----AVNQRDADLVHFWDKFRKAPEGSPKKNEARK 371
Query: 348 APAYEVKKRNVGPARL 363
+ R +
Sbjct: 372 QVLEVMSHRMHIDDSV 387
>emb|CAC18100.1| putative legumain [Zea mays]
Length = 485
Score = 593 bits (1515), Expect = e-168
Identities = 78/380 (20%), Positives = 150/380 (38%), Gaps = 33/380 (8%)
Query: 1 MSPYFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLG 60
+ P L + + WAVL+A S ++NYRH A++ Y+ +K+ G
Sbjct: 25 LEPTIRLPSER----AAADETDDDAVGTRWAVLIAGSNGYYNYRHQADICHAYQIMKKGG 80
Query: 61 IPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-L 119
+ D I++ + DD+A +P N PG + N + D+Y + DY G EV V+NF + L
Sbjct: 81 LKDENIVVFMYDDIAHSPENPRPGVII-NHPQGGDVYA-GVPKDYTGREVNVDNFFAVLL 138
Query: 120 TDRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKR 179
++ K + S ++ V+ + HGG L + DL D +
Sbjct: 139 GNKTALRGGSGKVVDSGPNDHIFVFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGT 198
Query: 180 YHELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVI 230
Y L+F ++ C++ +++ N+ AT +S ++SS+ + + +
Sbjct: 199 YKSLVFYLEACESGSIFEGLLPNDINVYATTASNAEESSWGTYCPGEFPSPPPEYDTCLG 258
Query: 231 DRWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRL 286
D Y + ++E+ ++L Y+ K + + G E +
Sbjct: 259 D---LYSVAWMEDSDFHNLRTESLK---QQYNLVKDRTAVQDTFSYGSHVMQYGSLELNV 312
Query: 287 RTVVDFFGNVQSVE--IEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAV-ANSTSQQE 343
+ + + G + + D P K + Q F Q Y A+S+ ++
Sbjct: 313 KHLFSYIGTNPANDDNTSIEDNSLPSFSK----AVNQRDADLVYFWQKYRKLADSSHEKN 368
Query: 344 ETRSAPAYEVKKRNVGPARL 363
E R + R+ + +
Sbjct: 369 EARRELLEVMAHRSHVDSSV 388
>gb|AAF89646.1| seed maturation protein PM40 [Glycine max]
Length = 496
Score = 593 bits (1513), Expect = e-168
Identities = 71/376 (18%), Positives = 140/376 (36%), Gaps = 33/376 (8%)
Query: 4 YFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPD 63
L A P + E + WAVLVA S + NYRH A+V Y+ + + G+ +
Sbjct: 41 VIKLPAEP------VDADSDHEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGLKE 94
Query: 64 SQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LTDR 122
I++ + DD+A + N PG + N D+Y + DY G VT +N + L D+
Sbjct: 95 ENIVVFMYDDIATDELNPRPGVII-NHPEGQDVYA-GVPKDYTGENVTAQNLFAVILGDK 152
Query: 123 LDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHE 182
K + S + +Y + HGG L + + A D + + Y +
Sbjct: 153 NKVKGGSGKVINSKPEDRIFIYYSDHGGPGVLGMPNMPYLYAMDFIEVLKKKHASGGYKK 212
Query: 183 LLFMIDTCQANT-MYTHFYSPN--IIATGSSELDQSSYSHHA-------DNDVGVAVIDR 232
++ ++ C++ + + I T +S ++S+ + + + D
Sbjct: 213 MVIYVEACESGNHVLKGIMPKDLQIYVTTASNAQENSWGTYCPGMDPSPPPEYITCLGD- 271
Query: 233 WTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRLRT 288
Y + ++E+ T ++++ Y K + N + + G+
Sbjct: 272 --LYSVAWMEDSETHNLKRESVK---QQYKSVKQRTSNFNNYAMGSHVMQYGDTNITAEK 326
Query: 289 VVDFFGNVQSVEIEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQE-ETRS 347
+ + G + G L+ + + Q + Q Y +N +++ +
Sbjct: 327 LYLYQGFDPAAVNFPPQN---GRLETKMEVVNQRDAELFFMWQMYQRSNHQPEKKTDILK 383
Query: 348 APAYEVKKRNVGPARL 363
A VK R +
Sbjct: 384 QIAETVKHRKHIDGSV 399
>sp|P49046|LEGU_CANEN Legumain precursor (Asparaginyl endopeptidase)
pir||JX0344 legumain (EC 3.4.22.34) precursor - jack bean
dbj|BAA06596.1| asparaginyl endopeptidase [Canavalia ensiformis]
Length = 475
Score = 592 bits (1511), Expect = e-168
Identities = 75/375 (20%), Positives = 143/375 (38%), Gaps = 36/375 (9%)
Query: 4 YFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPD 63
L P E + WAVLVA S + NYRH A+V Y+ + + G+ +
Sbjct: 25 VIQLPTEP----------VDDEVGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKE 74
Query: 64 SQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LTDR 122
I++ + DD+A N N PG + N + D+Y + DY G +VT EN + L D+
Sbjct: 75 ENIVVFMYDDIAYNAMNPRPGVII-NHPQGPDVYA-GVPKDYTGEDVTPENLYAVILGDK 132
Query: 123 LDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHE 182
K + S+ + ++ + HGG L +A + A D D + Y E
Sbjct: 133 SKVKGGSGKVINSNPEDRIFIFYSDHGGPGVLGMPNAPFVYAMDFIDVLKKKHASGGYKE 192
Query: 183 LLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHADN-------DVGVAVIDRW 233
++ I+ C++ +++ NI T +S ++S+ + + + D
Sbjct: 193 MVIYIEACESGSIFEGIMPKDLNIYVTTASNAQENSFGTYCPGMNPPPPEEYVTCLGD-- 250
Query: 234 TYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRLRTV 289
Y + ++E+ T ++T+ Y + + + + G+ +
Sbjct: 251 -LYSVSWMEDSETHNLKRETVQ---QQYQSVRKRTSNSNSYRFGSHVMQYGDTNITAEKL 306
Query: 290 VDFFGNVQSVEIEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQE-ETRSA 348
+ G + G+L+ + + Q + Q Y +N +++
Sbjct: 307 YLYHGFDPATVNFPPHN---GNLEAKMEVVNQRDAELLFMWQMYQRSNHQPEKKTHILEQ 363
Query: 349 PAYEVKKRNVGPARL 363
VK RN +
Sbjct: 364 ITETVKHRNHLDGSV 378
>gb|AAK15049.1| asparaginyl endopeptidase [Vigna radiata]
Length = 483
Score = 591 bits (1509), Expect = e-167
Identities = 75/375 (20%), Positives = 139/375 (37%), Gaps = 35/375 (9%)
Query: 4 YFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPD 63
+ L + + WA+L A S ++NYRH A++ Y+ +++ G+ +
Sbjct: 32 FLRLPSDSGN--------DDNVKGTRWAILFAGSNGYWNYRHQADICHAYQILRKGGLKE 83
Query: 64 SQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFI-RLLTDR 122
II+ + DD+A N N PG + N D+Y + + DY G + T NF LL D+
Sbjct: 84 ENIIVFMYDDIAFNWDNPRPGVII-NKPDGDDVY-EGVPKDYTGEDATAHNFYSALLGDK 141
Query: 123 LDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHE 182
K + S + ++ + HGG L I A DL + + Y
Sbjct: 142 SALTGGSGKVVNSGPDDRIFIFYSDHGGPGVLGTPAGPYIYASDLVEVLKKKHASGTYKN 201
Query: 183 LLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVIDRW 233
L+F ++ C+A +++ NI AT +S ++SS+ + + + D
Sbjct: 202 LVFYLEACEAGSIFEGLLPEDINIYATTASNAEESSWGTYCPGEYPSPPPEYSTCLGD-- 259
Query: 234 TYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHSNPG--VRWDLFPGGEQEGRLRTVVD 291
Y + ++E+ ++ L Y K + G + G+ E T+
Sbjct: 260 -LYSVAWMEDSDRHNLRTES---LHQQYKVVKDRTLSGGWYGSHVMQYGDVEFSKDTLFL 315
Query: 292 FFGNVQSVEI--EKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTS-QQEETRSA 348
+ G + + + S + Q F + A S ++ E R
Sbjct: 316 YLGTDPANDNLTFVDENSLWSSS----TAVNQRDADLVHFWHKFRKAPEGSPKKNEARKQ 371
Query: 349 PAYEVKKRNVGPARL 363
+ R +
Sbjct: 372 VLEVMSHRMHIDDSV 386
>ref|NP_918390.1| asparaginyl endopeptidase [Oryza sativa (japonica cultivar-group)]
dbj|BAA84650.1| asparaginyl endopeptidase [Oryza sativa]
dbj|BAB85400.1| asparaginyl endopeptidase [Oryza sativa (japonica cultivar-group)]
Length = 501
Score = 591 bits (1508), Expect = e-167
Identities = 82/377 (21%), Positives = 146/377 (37%), Gaps = 30/377 (7%)
Query: 5 FGLQALPFL-LSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPD 63
L + + A A + WAVL+A S ++NYRH A+V Y+ +KR G+ D
Sbjct: 40 LRLPSERAVAAGAAADDAAEAAEGTRWAVLIAGSNGYYNYRHQADVCHAYQIMKRGGLKD 99
Query: 64 SQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LTDR 122
II+ + DD+A NP N PG + N + D+Y + DY G EV V+N + L ++
Sbjct: 100 ENIIVFMYDDIAHNPENPRPGVII-NHPQGGDVYA-GVPKDYTGKEVNVKNLFAVLLGNK 157
Query: 123 LDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHE 182
K L S ++ ++ + HGG L + DL D + Y
Sbjct: 158 TAVKGGSGKVLDSGPNDHIFIFYSDHGGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKS 217
Query: 183 LLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVIDRW 233
L+F ++ C++ +++ N+ AT +S D+SS+ + + + D
Sbjct: 218 LVFYLEACESGSIFEGLLPNGINVYATTASNADESSWGTYCPGEYPSPPPEYDTCLGD-- 275
Query: 234 TYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRLRTV 289
Y + ++E+ ++L Y+ K + + G E V
Sbjct: 276 -LYSVAWMEDSDVHNLRTESLK---QQYNLVKERTSVQHTYYSGSHVMEYGSLELNAHHV 331
Query: 290 VDFFGNVQSVEI--EKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTS-QQEETR 346
+ G+ + + D P + + Q F Q Y +S ++ E R
Sbjct: 332 FMYMGSNPANDNATFVEDNSLPSFSR----AVNQRDADLVYFWQKYRKLPESSPEKNEAR 387
Query: 347 SAPAYEVKKRNVGPARL 363
+ R+ +
Sbjct: 388 KQLLEMMAHRSHVDNSV 404
>dbj|BAC54829.1| vacuolar processing enzyme-2 [Nicotiana tabacum]
Length = 484
Score = 589 bits (1504), Expect = e-167
Identities = 82/373 (21%), Positives = 150/373 (39%), Gaps = 29/373 (7%)
Query: 8 QALPFLLSLLFA-VFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQI 66
LP S F+ + + WAVLVA SR ++NYRH A+V Y+ +K+ G+ D I
Sbjct: 27 LKLPSEASTFFSGNYDDDSIGTKWAVLVAGSRGYWNYRHQADVCHAYQLLKKGGLKDENI 86
Query: 67 ILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LTDRLDE 125
I+ + DD+A N N PG + N+ D+Y + DY G+ VT NF+ + L ++
Sbjct: 87 IVFMYDDIAHNFENPRPGVII-NSPNGDDVY-KGVPKDYTGHHVTANNFLAVILGNKAAL 144
Query: 126 DVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELLF 185
K + S ++ ++ + HGG L + A DL D + Y L+F
Sbjct: 145 SGGSGKVVESGPNDHIFIFYSDHGGPGVLGMPSGPYLYADDLIDVLKRKHASGTYKSLVF 204
Query: 186 MIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVIDRWTYY 236
I+ C++ +++ NI AT +S ++ S+ + + + D Y
Sbjct: 205 YIEACESGSIFEGLLPEGLNIYATTASNAEEDSWGTYCPGDYPGPPPEYQTCLGD---LY 261
Query: 237 VLEFLENRVTSLDSKQTLGDLFDSYDETKIHSN---PGVRWDLFPGGEQEGRLRTVVDFF 293
+ ++E+ ++TLG Y+ K + P + G+ + + +
Sbjct: 262 AVSWMEDSEKHNLRRETLGM---QYELVKRRTANSFPYASSHVMQYGDLKLMDDPLSLYM 318
Query: 294 GNVQSVEI--EKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTS-QQEETRSAPA 350
G + + + S K + Q F + A S ++ E + +
Sbjct: 319 GTNPANDNYTFLDENSSLLSAKP----VNQRDADLLHFWDKFLKAPQGSVRKVEAQKQLS 374
Query: 351 YEVKKRNVGPARL 363
+ R +
Sbjct: 375 EAMSHRMHIDDSI 387
>emb|CAB16318.1| cysteine proteinase precursor [Vicia narbonensis]
Length = 488
Score = 589 bits (1504), Expect = e-167
Identities = 76/377 (20%), Positives = 148/377 (39%), Gaps = 32/377 (8%)
Query: 2 SPYFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGI 61
P L P E + WAVLVA S + NYRH A+V Y+ + + G+
Sbjct: 32 EPVIRLPGEP------VDADVEDEMGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGV 85
Query: 62 PDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LT 120
+ I++ + DD+A N N PG + N + ++Y D + DY G VT ENF + L
Sbjct: 86 KEENIVVFMYDDIAYNEMNPRPGVII-NHPQGPNVY-DGVPKDYNGDFVTAENFYAVILG 143
Query: 121 DRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRY 180
D+ K + S A + +Y + HGG L + + A D D + Y
Sbjct: 144 DKSKVRGGSGKVINSKAEDRIFIYCSDHGGPGVLGMPNMPYVYAMDFIDVLKKKHASGGY 203
Query: 181 HELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVID 231
+++ ++ C++ +++ N+ T +S ++S+ + + + D
Sbjct: 204 KKMVIYVEACESGSIFEGIMPKDINVYVTTASNAQENSWGTYCPGVDPAPPPEYITCLGD 263
Query: 232 RWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRLR 287
Y + ++E+ T ++T+ Y + + N + + G+
Sbjct: 264 ---LYSVAWMEDSETHNLKRETVK---QQYMSVRERTSNYKNYPLGSHVMQYGDTNITDE 317
Query: 288 TVVDFFGNVQSVEIEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANS-TSQQEETR 346
+ + G + ++ D L+ + + Q + Y + T ++++T
Sbjct: 318 KLYLYHGFDPADGEPSSNNDI---LEAKMEVVNQRDAEILFMWHMYQRLDHQTEKKKDTL 374
Query: 347 SAPAYEVKKRNVGPARL 363
+ VK RN +
Sbjct: 375 EKISETVKHRNHLDGSV 391
>dbj|BAA76745.1| asparaginyl endopeptidase (VmPE-1A) [Vigna mungo]
Length = 482
Score = 588 bits (1502), Expect = e-167
Identities = 74/375 (19%), Positives = 138/375 (36%), Gaps = 35/375 (9%)
Query: 4 YFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPD 63
+ L + + WA+L A S ++NYRH A++ Y+ +++ G+ +
Sbjct: 31 FLRLPSDSGN--------DDNVQGTRWAILFAGSNGYWNYRHQADICHAYQILRKGGLKE 82
Query: 64 SQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFI-RLLTDR 122
II+ + DD+A N N PG + N D+Y + + DY G + T NF LL D+
Sbjct: 83 ENIIVFMYDDIAFNWDNPRPGVII-NKPDGDDVY-EGVPKDYTGEDATAHNFYSALLGDK 140
Query: 123 LDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHE 182
K + S + ++ + HGG L I A DL + + Y
Sbjct: 141 SALTGGSGKVVSSGPDDRIFIFYSDHGGPGVLGTPAGPYIYASDLVEVLKKKHASGTYKN 200
Query: 183 LLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGVAVIDRW 233
L+F ++ C+A +++ NI AT +S ++SS+ + + + D
Sbjct: 201 LVFYLEACEAGSIFEGLLPEDINIYATTASNAEESSWGTYCPGEYPSPPPEYSTCLGD-- 258
Query: 234 TYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHSNPG--VRWDLFPGGEQEGRLRTVVD 291
Y + ++E+ ++ L Y K + G + G+ E +
Sbjct: 259 -LYSVAWMEDSDRHNLRTES---LHQQYKVVKDRTLSGGWYGSHVMQYGDVEFSKDALFL 314
Query: 292 FFGNVQSVEI--EKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTS-QQEETRSA 348
+ G + + + S + Q F + A S ++ E R
Sbjct: 315 YLGTDPANDNLTFVDENSLWSSS----TAVNQRDADLVHFWHKFRKAPEGSPKKNEARKQ 370
Query: 349 PAYEVKKRNVGPARL 363
+ R +
Sbjct: 371 VLEVMSHRMHIDDSV 385
>ref|NP_176458.1| beta-VPE [Arabidopsis thaliana]
sp|Q39044|VPEB_ARATH Vacuolar processing enzyme, beta-isozyme precursor (Beta-VPE)
gb|AAK56243.1| At1g62710/F23N19_8 [Arabidopsis thaliana]
gb|AAL15381.1| At1g62710/F23N19_8 [Arabidopsis thaliana]
Length = 486
Score = 586 bits (1497), Expect = e-166
Identities = 77/377 (20%), Positives = 140/377 (36%), Gaps = 30/377 (7%)
Query: 2 SPYFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGI 61
P + A + WAVLVA S + NYRH A+V Y+ +++ G+
Sbjct: 28 EPKILMPTEEAN----PADQDEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGL 83
Query: 62 PDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LT 120
+ I++++ DD+A +P N PGT+ N D+Y + DY G VT NF + L
Sbjct: 84 KEENIVVLMYDDIANHPLNPRPGTLI-NHPDGDDVYA-GVPKDYTGSSVTAANFYAVLLG 141
Query: 121 DRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRY 180
D+ K + S ++ VY HGG L + I A D + + Y
Sbjct: 142 DQKAVKGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTY 201
Query: 181 HELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHADN-------DVGVAVID 231
E++ ++ C++ +++ NI T +S +SSY + + + D
Sbjct: 202 KEMVIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSYGTYCPGMNPSPPSEYITCLGD 261
Query: 232 RWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRLR 287
Y + ++E+ T K+T+ Y K+ + + G +
Sbjct: 262 ---LYSVAWMEDSETHNLKKETIK---QQYHTVKMRTSNYNTYSGGSHVMEYGNNSIKSE 315
Query: 288 TVVDFFGNVQSVEIEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQE-ETR 346
+ + G + + P K + Q Y + S+++ +T
Sbjct: 316 KLYLYQGFDPATVNLPL-NELPVKSKIG--VVNQRDADLLFLWHMYRTSEDGSRKKDDTL 372
Query: 347 SAPAYEVKKRNVGPARL 363
+ R A +
Sbjct: 373 KELTETTRHRKHLDASV 389
>pir||T10944 cysteine proteinase (EC 3.4.22.-) precursor - spring vetch
emb|CAA07639.1| cysteine proteinase precursor [Vicia sativa]
Length = 503
Score = 584 bits (1492), Expect = e-165
Identities = 76/378 (20%), Positives = 150/378 (39%), Gaps = 33/378 (8%)
Query: 2 SPYFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGI 61
P L P E + WAVLVA S + NYRH A+V Y+ + + G+
Sbjct: 46 EPVIRLPGEP------VDADVEDEIGTRWAVLVAGSNGYGNYRHQADVCHAYQLLIKGGV 99
Query: 62 PDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LT 120
+ I++ + DD+A + N PG + N + ++Y D + DY G VT +N + L
Sbjct: 100 KEENIVVFMYDDIAYSEFNPRPGVII-NHPQGPNVY-DGVPKDYTGDFVTADNLYAVILG 157
Query: 121 DRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRY 180
D+ K + S A + +Y + HGG L + + A D D + + Y
Sbjct: 158 DKSKVRGGSGKVINSKAEDRIFIYYSDHGGPGVLGMPNMPYVYAMDFIDVLKKKHASRGY 217
Query: 181 HELLFMIDTCQANTMYTHFYSPNI--IATGSSELDQSSYSHH--------ADNDVGVAVI 230
+++ ++ C++ +++ +I T +S ++SS+ + + + +
Sbjct: 218 QQMVIYVEACESGSVFQGIMPKDIDVYVTTASNAEESSWGTYLVPGVYPASPPEYITCLG 277
Query: 231 DRWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRL 286
D Y + ++E+ T ++TL + K + N G+ + G+
Sbjct: 278 D---LYSVAWMEDSETHNLKRETLK---QQFASVKERTLNNNNYGLGSHVTEYGDTNITD 331
Query: 287 RTVVDFFGNVQSVEIEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQE-ET 345
+ + G + + G L+ + + Q + Q Y + S+++ +
Sbjct: 332 EKLYLYHGFDPASVNLPPNN---GRLESKMEVVNQRDAEILFMWQMYQRLDHQSEKKRDI 388
Query: 346 RSAPAYEVKKRNVGPARL 363
+ VK RN +
Sbjct: 389 LKKISETVKHRNHLDGSV 406
>gb|AAF89679.1| asparaginyl endopeptidase [Sesamum indicum]
Length = 489
Score = 578 bits (1475), Expect = e-163
Identities = 67/358 (18%), Positives = 139/358 (38%), Gaps = 28/358 (7%)
Query: 22 ASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQIILMLPDDMACNPRNA 81
+ ++ + WAVLVA S + NYRH A+V Y+ +K+ G+ D II+ + DD+A N N
Sbjct: 47 ETEDNATRWAVLVAGSNGFGNYRHQADVCHAYQILKKGGLRDENIIVFMYDDIAMNELNP 106
Query: 82 FPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LTDRLDEDVPRSKRLGSDAGSN 140
G + N D+Y + DY G +VT EN + L D+ K + S
Sbjct: 107 RKGVII-NHPTGGDVYA-GVPKDYTGEQVTAENLYAVILGDKSAIKGGSGKVVDSKPNDR 164
Query: 141 VLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELLFMIDTCQANTMYTHFY 200
+ +Y + HGG L + + A D + + Y E++ ++ C++ +++
Sbjct: 165 IFIYYSDHGGPGVLGMPNMPYLYANDFIEVLKKKHASGTYKEMVIYVEACESGSVFEGLM 224
Query: 201 SP--NIIATGSSELDQSSYSHHADN-------DVGVAVIDRWTYYVLEFLENRVTSLDSK 251
+I T +S ++SS+ + + + D Y + ++E+ + +
Sbjct: 225 PDDLDIYVTTASNAEESSWGTYCPGMDPPPPPEYITCLGD---LYSVAWMEDSESHNLKR 281
Query: 252 QTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRLRTVVDFFGNVQSVEI-EKADA 306
+T+ Y + K + + G + + + + G + E ++
Sbjct: 282 ETVE---QQYQQVKERTSNFNTYNAGSHVMEYGNKSIKSEKLYLYQGFDPATENMPPSEN 338
Query: 307 DEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQE-ETRSAPAYEVKKRNVGPARL 363
+ + Q + Y + ++++ E + R +
Sbjct: 339 HLKPHMDV----VNQRDADLLFLWERYKRLDGGAKKKSELFKLITDTMLHRKHMDDSI 392
>emb|CAC43295.1| putative vacuolar processing enzyme [Beta vulgaris]
Length = 486
Score = 577 bits (1473), Expect = e-163
Identities = 72/357 (20%), Positives = 139/357 (38%), Gaps = 26/357 (7%)
Query: 22 ASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQIILMLPDDMACNPRNA 81
+ WAVL+A S ++NYRH A+V Y+ +K+ G+ D II+ + DD+A + N
Sbjct: 44 DDDSVGTRWAVLIAGSSGYWNYRHQADVCHAYQVLKKGGLKDENIIVFMYDDIAYDEENP 103
Query: 82 FPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFI-RLLTDRLDEDVPRSKRLGSDAGSN 140
PG + N+ D+Y + DY G +VTV NF +L ++ K + S +
Sbjct: 104 RPGVLI-NSPYGHDVYA-GVPKDYTGEDVTVNNFFAAILGNKDAITGGSGKVVNSGPNDH 161
Query: 141 VLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELLFMIDTCQANTMYTHFY 200
+ ++ + HGG L + A +L + + Y L+ I+ C++ +++
Sbjct: 162 IFIFYSDHGGAGVLGMPTYPYLYADELIETLKEKHASGTYKSLVVYIEACESGSIFEGIL 221
Query: 201 SP--NIIATGSSELDQSSYSHHA-------DNDVGVAVIDRWTYYVLEFLENRVTSLDSK 251
NI AT +S +SS+ + + + D Y + ++E+
Sbjct: 222 PEGLNIYATTASNAVESSWGTYCPGQDPNVPPEYDTCLGD---LYSVSWIEDSERHNLHT 278
Query: 252 QTLGDLFDSYDETKIHSN--PGVRWDLFPGGEQEGRLRTVVDFFGNVQSVEI--EKADAD 307
++L Y+ K + P + G++E + + G + E D
Sbjct: 279 ESLK---QQYEVVKTKTAEKPFYGSHVMQYGDKELTQDMLYLYMGTNPNNENYTYVDDNS 335
Query: 308 EPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTS-QQEETRSAPAYEVKKRNVGPARL 363
+ + Q F + A+ S ++ + + R +
Sbjct: 336 LHPTSSN---AVNQRDADLIHFWNKFRKASEGSQRKINAQKQFMEVMSHRVHLDDSI 389
>pir||S60050 vacuolar processing enzyme (EC 3.4.22.-) isozyme beta precursor -
Arabidopsis thaliana
dbj|BAA09615.1| vacuolar processing enzyme [Arabidopsis thaliana]
Length = 484
Score = 576 bits (1471), Expect = e-163
Identities = 75/375 (20%), Positives = 140/375 (37%), Gaps = 28/375 (7%)
Query: 2 SPYFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGI 61
P + A + WAVLVA S + NYRH A+V Y+ +++ G+
Sbjct: 28 EPKILMPTEEAN----PADQDEDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGL 83
Query: 62 PDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRL-LT 120
+ I++++ DD+A +P N PGT+ N D+Y + DY G VT NF + L
Sbjct: 84 KEENIVVLMYDDIANHPLNPRPGTLI-NHPDGDDVYA-GVPKDYTGSSVTAANFYAVLLG 141
Query: 121 DRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRY 180
D+ K + S ++ VY HGG L + I A D + + Y
Sbjct: 142 DQKAVKGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTY 201
Query: 181 HELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHADN-------DVGVAVID 231
E++ ++ C++ +++ NI T +S +SSY + + + D
Sbjct: 202 KEMVIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSYGTYCPGMNPSPPSEYITCLGD 261
Query: 232 RWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKI--HSNPGVRWDLFPGGEQEGRLRTV 289
Y + ++E+ T K+T+ Y + ++ + G + +
Sbjct: 262 ---LYSVAWMEDSETHNLKKETIK---QQYHTMRTSNYNTYSGGSHVMEYGNNSIKSEKL 315
Query: 290 VDFFGNVQSVEIEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQE-ETRSA 348
+ G + + P K + Q + + S+++ +T
Sbjct: 316 YLYQGFEPATVNLPL-NELPAKSKIG--VVNQRDADLLFLWHMHRTSEDGSRKKDDTLKE 372
Query: 349 PAYEVKKRNVGPARL 363
+ R A +
Sbjct: 373 LTETTRHRKHLDASV 387
>dbj|BAC76418.1| vacuolar processing enzyme [Oryza sativa (japonica cultivar-group)]
emb|CAE03021.2| OSJNBa0091D06.14 [Oryza sativa (japonica cultivar-group)]
Length = 497
Score = 571 bits (1458), Expect = e-161
Identities = 71/381 (18%), Positives = 145/381 (37%), Gaps = 31/381 (8%)
Query: 2 SPYFGLQALPFLLSLLFAVFASAEH----TSNWAVLVATSRFWFNYRHLANVLSLYRTVK 57
P + + A + + WAVLVA S + NYRH A+V Y+ ++
Sbjct: 31 EPLIRMPTEEGDDAEAAAPAPAPAAADYGGTRWAVLVAGSSGYGNYRHQADVCHAYQILQ 90
Query: 58 RLGIPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIR 117
+ G+ + I++ + DD+A N N PGT+ N + D+Y + DY G++VT ENF
Sbjct: 91 KGGVKEENIVVFMYDDIAHNILNPRPGTII-NHPKGGDVYA-GVPKDYTGHQVTTENFFA 148
Query: 118 L-LTDRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWE 176
+ L ++ K + S ++ +Y + HGG L + + A D +
Sbjct: 149 VLLGNKTAVTGGSGKVIDSKPEDHIFIYYSDHGGPGVLGMPNLPYLYAGDFIKVLQKKHA 208
Query: 177 KKRYHELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA-------DNDVGV 227
Y +++ ++ C++ +++ NI T +S ++S+ + +
Sbjct: 209 SNSYSKMVIYVEACESGSIFEGLMPENLNIYVTTASNAVENSWGTYCPGEEPSPPPEYIT 268
Query: 228 AVIDRWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHSNPGV----RWDLFPGGEQE 283
+ D Y + ++E+ T K+T+ D Y+ K ++ + G++
Sbjct: 269 CLGD---MYSVAWMEDSETHNLKKETIED---QYELVKKRTSNANKLNEGSHVMEYGDKT 322
Query: 284 GRLRTVVDFFGNVQSVEIEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQ-Q 342
+ + + G + + P + Q + Y N S+ +
Sbjct: 323 FKDEKLFLYQGFNPANGNITNELIWP----VPKATVNQRDADLLFMWKRYEQLNGVSEDK 378
Query: 343 EETRSAPAYEVKKRNVGPARL 363
+ R + +
Sbjct: 379 LRALREIEDTIAHRKHLDSSI 399
>ref|XP_327545.1| hypothetical protein [Neurospora crassa]
gb|EAA32877.1| hypothetical protein [Neurospora crassa]
Length = 401
Score = 547 bits (1397), Expect = e-154
Identities = 262/397 (65%), Positives = 305/397 (75%), Gaps = 8/397 (2%)
Query: 1 MSPYFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLG 60
+S L AL + L A AEHTSNWAVLV TSRFWFNYRHLANVLS+YRTVKRLG
Sbjct: 3 LSNILRLPALLGAMLLGTAAVVEAEHTSNWAVLVCTSRFWFNYRHLANVLSIYRTVKRLG 62
Query: 61 IPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRLLT 120
IPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRLLT
Sbjct: 63 IPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRLLT 122
Query: 121 DRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRY 180
DR+ +++PRSKRL +D SN+LVYMTGHGG++FLKFQDAEEIGA+DLADAF QMWEKKRY
Sbjct: 123 DRVGDEMPRSKRLLTDDRSNILVYMTGHGGNEFLKFQDAEEIGAFDLADAFEQMWEKKRY 182
Query: 181 HELLFMIDTCQANTMYTHFYSPNIIATGSSELDQSSYSHHADNDVGVAVIDRWTYYVLEF 240
HE+LFMIDTCQANTMY+ YSPNIIATGSSELDQSSYSHHADND+GVAVIDR+TYY LEF
Sbjct: 183 HEILFMIDTCQANTMYSKLYSPNIIATGSSELDQSSYSHHADNDIGVAVIDRYTYYNLEF 242
Query: 241 LENRVTSLDSKQTLGDLFDSYDETKIHSNPGVRWDLFPGGEQEGRLRTVVDFFGNVQSVE 300
LEN V SK+T+GDLFDSY KIHSN GVR+DLFPGG + R R V DFFGNVQ+VE
Sbjct: 243 LENEVKDTSSKKTVGDLFDSYTYEKIHSNAGVRYDLFPGGAEAARSRLVTDFFGNVQNVE 302
Query: 301 IEKADADEPGSLKEDLIEIAQLVEKWRTFS---QDYAVANSTSQQEETRSAPAYE--VKK 355
+ D + +L+EDL+ +++ + R + + +V + + ET+ E +
Sbjct: 303 V---DGSKNTTLEEDLLALSKTIAALREKAGEEEAISVGKGNAARNETKKETKNETLLTH 359
Query: 356 RNVGPARLTDETPWEKRLIGLSILGACAAVWFTGSTL 392
++ LTD+ K+L L + CA +W GS L
Sbjct: 360 KSQFAKPLTDDDWLVKKLSALGAVVGCAVLWQMGSWL 396
>gb|AAL40390.1| C13 cysteine proteinase precursor [Oryza sativa subsp. indica]
Length = 465
Score = 534 bits (1362), Expect = e-150
Identities = 62/377 (16%), Positives = 141/377 (36%), Gaps = 41/377 (10%)
Query: 9 ALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQIIL 68
A S + WAVLVA S + NYRH A+V Y+ +++ G+ + I++
Sbjct: 11 ATVPRGSEGTEEEEKDGVGTRWAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVV 70
Query: 69 MLPDDMACNPRNAFPGTV-YSNADRAVDLYGDNIEVDYRGYEVTVE------NFIRL-LT 120
+ DD+A N N PG + + V ++ +T+E NF + L
Sbjct: 71 FMYDDIANNILNPRPGLLSIIHR---VKMFMQEF------RRITLETKSLQKNFYAVLLG 121
Query: 121 DRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRY 180
++ K + S ++ ++ + HGG L + + A D + Y
Sbjct: 122 NKTAVTGGSRKVIDSKPNDHIFIFYSDHGGPGVLGMPNLPYLYAADFMKVLQEKHASNTY 181
Query: 181 HELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHADN-------DVGVAVID 231
+++ ++ C++ +++ NI T +S ++SS+ + + + D
Sbjct: 182 AKMVIYVEACESGSIFEGLMPEDLNIYVTTASNAEESSWGTYCPGMEPSPPSEYITCLGD 241
Query: 232 RWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NPGVRWDLFPGGEQEGRLR 287
Y + ++E+ T ++++ Y+ K + + G + G++ +
Sbjct: 242 ---LYSVSWMEDSETHNLKEESIK---KQYEVVKKRTSDMNSYGAGSHVMEYGDRTFKDD 295
Query: 288 TVVDFFGNVQSVEIEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVA-NSTSQQEETR 346
+ + G + A+ S + Q + Y + + + ++ +
Sbjct: 296 KLYLYQGFDPAN----AEVKNKLSWEGPKAAANQRDADLLFLWRRYELLHDKSEEKLKAL 351
Query: 347 SAPAYEVKKRNVGPARL 363
+ V R + + +
Sbjct: 352 REISDTVMHRKLLDSSV 368
>emb|CAB42655.1| putative preprolegumain [Vicia narbonensis]
Length = 380
Score = 533 bits (1359), Expect = e-150
Identities = 63/332 (18%), Positives = 125/332 (36%), Gaps = 28/332 (8%)
Query: 48 NVLSLYRTVKRLGIPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRG 107
+V Y+ +++ G+ + II+ + DD+A + N PG + N+ ++Y + + DY G
Sbjct: 1 DVCHAYQLLRKGGLKEENIIVFMYDDIAYSEENPRPGVII-NSPHGENVY-EGVPKDYTG 58
Query: 108 YEVTVENFI-RLLTDRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWD 166
+VTV NF LL ++ K + S + V+ + HGG L + + A D
Sbjct: 59 EDVTVGNFFAALLGNKSALSGGSGKVVDSGPNDRIFVFYSDHGGPGVLGMPTSPYMYASD 118
Query: 167 LADAFGQMWEKKRYHELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA--- 221
L + Y L+F ++ C++ +++ NI AT ++ ++SS+ +
Sbjct: 119 LVEVLKIKHAAGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTAANAEESSWGTYCPGE 178
Query: 222 ----DNDVGVAVIDRWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS---NPGVRW 274
+ + D Y + ++E+ +T L Y+ K + N
Sbjct: 179 NPSPPPEYETCLAD---LYSVAWMEDSDIHNLQTET---LHQQYELVKERTSNGNSNYGS 232
Query: 275 DLFPGGEQEGRLRTVVDFFGNVQSVEI--EKADADEPGSLKEDLIEIAQLVEKWRTFSQD 332
+ G+ E ++ + G+ S E K I Q F
Sbjct: 233 HVMQYGDIELSKDSLFLYLGSNPSNENFTFVGRNSLVPPSK----AINQRDADLIHFWDK 288
Query: 333 YAVANSTS-QQEETRSAPAYEVKKRNVGPARL 363
+ A S ++ + + R +
Sbjct: 289 FRKAPQGSPRKAAAQKEVLEAMSHRMHIDDSI 320
>emb|CAB42651.1| putative preprolegumain [Nicotiana tabacum]
Length = 419
Score = 528 bits (1347), Expect = e-148
Identities = 63/334 (18%), Positives = 129/334 (37%), Gaps = 27/334 (8%)
Query: 45 HLANVLSLYRTVKRLGIPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVD 104
H A+V Y+ +KR G+ D I++ + DD+A + N PG + N D+Y + D
Sbjct: 1 HQADVCHAYQILKRGGLKDENIVVFMYDDIAKSELNPRPGVII-NHPNGSDVYA-GVPKD 58
Query: 105 YRGYEVTVENFIRL-LTDRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIG 163
Y G VT N + L D+ K + S + +Y + HGG L + +
Sbjct: 59 YTGEHVTAANLYAVLLGDKSAVKGGSGKIVDSKPNDRIFLYYSDHGGPGVLGMPNMPFLY 118
Query: 164 AWDLADAFGQMWEKKRYHELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA 221
A D + + Y E++ I+ C++ +++ NI T +S ++SS+ +
Sbjct: 119 AKDFIEVLKKKHAAGTYKEMVLYIEACESGSVFEGMMPEDLNIYVTTASNAEESSWGTYC 178
Query: 222 DN-------DVGVAVIDRWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHS----NP 270
+ + D Y + ++E+ + K+T+ Y++ K + N
Sbjct: 179 PGMDPPPPPEYITCLGD---LYSVAWMEDSESHNLKKETIK---QQYEKVKERTSNFNNY 232
Query: 271 GVRWDLFPGGEQEGRLRTVVDFFGNVQSVEIEKADADEPGSLKEDLIEIAQLVEKWRTFS 330
+ G +E + V + G + A+ ++ + Q
Sbjct: 233 NAGSHVMEYGSKEIKPEKVYLYQGFDPATANLSANKIAFAHVEV----VNQRDADLLFLW 288
Query: 331 QDY-AVANSTSQQEETRSAPAYEVKKRNVGPARL 363
+ Y +A+++ ++ + R + R +
Sbjct: 289 ERYKELADNSLEKAKLRKEITDTMLHRKHLDGSV 322
>dbj|BAA09530.1| cysteine protease [Homo sapiens]
Length = 433
Score = 522 bits (1333), Expect = e-147
Identities = 92/355 (25%), Positives = 152/355 (41%), Gaps = 21/355 (5%)
Query: 10 LPFLLSLLFAVFASAEH-TSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQIIL 68
L L + E +WAV+VA S W+NYRH A+ Y+ + R GIPD QI++
Sbjct: 9 LSVALGIGAVPIDDPEDGGKHWAVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVV 68
Query: 69 MLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRLL-TDRLDEDV 127
M+ DD+A + N PG V N D+Y + DY G +VT +NF+ +L D
Sbjct: 69 MMYDDIAYSEDNPTPGIVI-NRPNGTDVY-QGVPKDYTGEDVTPQNFLAVLRGDAEAVKG 126
Query: 128 -PRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELLFM 186
K L S +V +Y T HG L F + E++ DL + M++ K Y +++F
Sbjct: 127 IGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYKHKMYRKMVFY 185
Query: 187 IDTCQANTMYTHFYSP-NIIATGSSELDQSSYSHHADNDVGVAVIDRWTYYVLEFLENRV 245
I+ C++ +M H N+ AT ++ +SSY+ + D + D +Y + ++E+
Sbjct: 186 IEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGD---WYSVNWMEDSD 242
Query: 246 TSLDSKQTLGDLFDSYDETKIHSNPGVRWDLFPGGEQEGRLRTVVDFFG----NVQSVEI 301
+K+T L Y K H+N + G + V+ F G V +
Sbjct: 243 VEDLTKET---LHKQYHLVKSHTNT---SHVMQYGNKTISTMKVMQFQGMKRKASSPVPL 296
Query: 302 EKA-DADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQEETRSAPAYEVKK 355
D S L + + + + + + + R V+K
Sbjct: 297 PPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLDARHLIEKSVRK 351
>sp|Q99538|LGMN_HUMAN Legumain precursor (Asparaginyl endopeptidase) (Protease, cysteine
1)
emb|CAA70989.1| legumain [Homo sapiens]
emb|CAD61906.1| unnamed protein product [Homo sapiens]
Length = 433
Score = 521 bits (1330), Expect = e-146
Identities = 91/355 (25%), Positives = 151/355 (41%), Gaps = 21/355 (5%)
Query: 10 LPFLLSLLFAVFASAEH-TSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQIIL 68
L L + E +W V+VA S W+NYRH A+ Y+ + R GIPD QI++
Sbjct: 9 LSVALGIGAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVV 68
Query: 69 MLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRLL-TDRLDEDV 127
M+ DD+A + N PG V N D+Y + DY G +VT +NF+ +L D
Sbjct: 69 MMYDDIAYSEDNPTPGIVI-NRPNGTDVY-QGVPKDYTGEDVTPQNFLAVLRGDAEAVKG 126
Query: 128 -PRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELLFM 186
K L S +V +Y T HG L F + E++ DL + M++ K Y +++F
Sbjct: 127 IGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYKHKMYRKMVFY 185
Query: 187 IDTCQANTMYTHFYSP-NIIATGSSELDQSSYSHHADNDVGVAVIDRWTYYVLEFLENRV 245
I+ C++ +M H N+ AT ++ +SSY+ + D + D +Y + ++E+
Sbjct: 186 IEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGD---WYSVNWMEDSD 242
Query: 246 TSLDSKQTLGDLFDSYDETKIHSNPGVRWDLFPGGEQEGRLRTVVDFFG----NVQSVEI 301
+K+T L Y K H+N + G + V+ F G V +
Sbjct: 243 VEDLTKET---LHKQYHLVKSHTNT---SHVMQYGNKTISTMKVMQFQGMKRKASSPVPL 296
Query: 302 EKA-DADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQEETRSAPAYEVKK 355
D S L + + + + + + + R V+K
Sbjct: 297 PPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLDARHLIEKSVRK 351
>ref|NP_005597.2| legumain; protease, cysteine, 1 (legumain) [Homo sapiens]
gb|AAH03061.1| Legumain [Homo sapiens]
Length = 433
Score = 521 bits (1328), Expect = e-146
Identities = 91/355 (25%), Positives = 151/355 (41%), Gaps = 21/355 (5%)
Query: 10 LPFLLSLLFAVFASAEH-TSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQIIL 68
L L + E +W V+VA S W+NYRH A+ Y+ + R GIPD QI++
Sbjct: 9 LSVALGIGAIPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVV 68
Query: 69 MLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRLL-TDRLDEDV 127
M+ DD+A + N PG V N D+Y + DY G +VT +NF+ +L D
Sbjct: 69 MMYDDIAYSEDNPTPGIVI-NRPNGTDVY-QGVPKDYTGEDVTPQNFLAVLRGDAEAVKG 126
Query: 128 -PRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELLFM 186
K L S +V +Y T HG L F + E++ DL + M++ K Y +++F
Sbjct: 127 IGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYKHKMYRKMVFY 185
Query: 187 IDTCQANTMYTHFYSP-NIIATGSSELDQSSYSHHADNDVGVAVIDRWTYYVLEFLENRV 245
I+ C++ +M H N+ AT ++ +SSY+ + D + D +Y + ++E+
Sbjct: 186 IEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGD---WYSVNWMEDSD 242
Query: 246 TSLDSKQTLGDLFDSYDETKIHSNPGVRWDLFPGGEQEGRLRTVVDFFG----NVQSVEI 301
+K+T L Y K H+N + G + V+ F G V +
Sbjct: 243 VEDLTKET---LHKQYHLVKSHTNT---SHVMQYGNKTISTMKVMQFQGMKRKASSPVPL 296
Query: 302 EKA-DADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQEETRSAPAYEVKK 355
D S L + + + + + + + R V+K
Sbjct: 297 PPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLDARHLIEKSVRK 351
>gb|AAH56842.1| MGC64351 protein [Xenopus laevis]
Length = 433
Score = 512 bits (1307), Expect = e-144
Identities = 99/380 (26%), Positives = 168/380 (44%), Gaps = 25/380 (6%)
Query: 4 YFGLQAL-PFLLSLLFAVFASAEH-TSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGI 61
+ L AL F+L F++ E +W VLVA S W+NYRH A+V Y+ VK+ GI
Sbjct: 2 FLHLAALVSFVLGASSVPFSNPEDTGKHWVVLVAGSNGWYNYRHQADVCHAYQIVKKNGI 61
Query: 62 PDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRLL-T 120
PD QI++M+ DD+A N N G + N D+Y + DY G +V +NF+ +L
Sbjct: 62 PDEQIVVMMYDDIANNDENPTKGVII-NRPNGTDVYA-GVLKDYIGDDVNPKNFLAVLSG 119
Query: 121 DRLDEDV-PRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKR 179
D K + S +V VY T HG L F ++++ +L M+E K+
Sbjct: 120 DSEAVKGKGSGKVIRSGPNDHVFVYFTDHGAPGLLAFP-SDDLHVMELNKTIQHMYENKK 178
Query: 180 YHELLFMIDTCQANTMYTHFYSP-NIIATGSSELDQSSYSHHADNDVGVAVIDRWTYYVL 238
Y +++F I+ C++ +M H + N+ AT ++ +SSY+ + D+ + D Y +
Sbjct: 179 YKKMVFYIEACESGSMMNHLPNNINVYATTAANPQESSYACYYDDKRDTYLGD---LYSV 235
Query: 239 EFLENRVTSLDSKQTLGDLFDSYDETKIHSNPGVRWDLFPGGEQEGRLRTVVDFFGNVQ- 297
++E+ +K+T L + K H+N + G + V F GNV+
Sbjct: 236 SWMEDSDMEDLAKET---LHKQFVLVKQHTNT---SHVMQYGNRTISQMKVNQFQGNVKI 289
Query: 298 ---SVEIEKA-DADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQEETRSAPAYEV 353
V +E D S L + + + Q A+ +E + +
Sbjct: 290 TSTPVYLEPVKHMDLTPSPDVPLAILKRKLMATNDILQARAIVREIKAHQEAKQLIKESM 349
Query: 354 KKRNVGPARLTDETPWEKRL 373
+K +T+ + +
Sbjct: 350 RK---IVNMVTESDELTEEI 366
>sp|Q9R0J8|LGMN_RAT Legumain precursor (Asparaginyl endopeptidase) (Protease, cysteine
1)
dbj|BAA84750.1| legumain [Rattus norvegicus]
Length = 435
Score = 512 bits (1305), Expect = e-144
Identities = 94/358 (26%), Positives = 153/358 (42%), Gaps = 21/358 (5%)
Query: 7 LQALPFLLSLLFAVFASAEH-TSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQ 65
L +L + E +W V+VA S W+NYRH A+ Y+ + R GIPD Q
Sbjct: 8 LLSLVLGAGAVHIGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQ 67
Query: 66 IILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRLL-TDRLD 124
II+M+ DD+A N N PG V N D+Y + DY G +VT ENF+ +L D
Sbjct: 68 IIVMMYDDIANNEENPTPGVVI-NRPNGTDVY-KGVPKDYTGEDVTPENFLAVLRGDEEA 125
Query: 125 EDV-PRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHEL 183
K L S +V VY T HG L F + E++ DL M+E K Y ++
Sbjct: 126 VKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNKTIRYMYEHKMYQKM 184
Query: 184 LFMIDTCQANTMYTHFYSP-NIIATGSSELDQSSYSHHADNDVGVAVIDRWTYYVLEFLE 242
+F I+ C++ +M H ++ AT ++ ++SSY+ + D + + D +Y + ++E
Sbjct: 185 VFYIEACESGSMMNHLPDDIDVYATTAANPNESSYACYYDEERSTYLGD---WYSVNWME 241
Query: 243 NRVTSLDSKQTLGDLFDSYDETKIHSNPGVRWDLFPGGEQEGRLRTVVDFFG----NVQS 298
+ +K+T L Y K H+N + G + V+ F G
Sbjct: 242 DSDVEDLTKET---LHKQYHLVKSHTNT---SHVMQYGNKSISTMKVMQFQGMKHRASSP 295
Query: 299 VEIEKA-DADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQEETRSAPAYEVKK 355
+ + D S L + + + + + + + R V+K
Sbjct: 296 ISLPPVTHLDLTPSPDVPLTILKRKLLRTNNMKESQVLVGQIQHLLDARHIIEKSVQK 353
>ref|NP_071562.2| protease, cysteine, 1 (legumain) [Rattus norvegicus]
gb|AAF73260.1| legumain [Rattus norvegicus]
Length = 435
Score = 512 bits (1305), Expect = e-143
Identities = 94/358 (26%), Positives = 153/358 (42%), Gaps = 21/358 (5%)
Query: 7 LQALPFLLSLLFAVFASAEH-TSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQ 65
L +L + E +W V+VA S W+NYRH A+ Y+ + R GIPD Q
Sbjct: 8 LLSLVLGAGAVHIGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQ 67
Query: 66 IILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRLL-TDRLD 124
II+M+ DD+A N N PG V N D+Y + DY G +VT ENF+ +L D
Sbjct: 68 IIVMMYDDIANNEENPTPGVVI-NRPNGTDVY-KGVPKDYTGEDVTPENFLAVLRGDEEA 125
Query: 125 EDV-PRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHEL 183
K L S +V VY T HG L F + E++ DL M+E K Y ++
Sbjct: 126 VKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNKTIRYMYEHKMYQKM 184
Query: 184 LFMIDTCQANTMYTHFYSP-NIIATGSSELDQSSYSHHADNDVGVAVIDRWTYYVLEFLE 242
+F I+ C++ +M H ++ AT ++ ++SSY+ + D + + D +Y + ++E
Sbjct: 185 VFYIEACESGSMMNHLPDDIDVYATTAANPNESSYACYYDEERSTYLGD---WYSVNWME 241
Query: 243 NRVTSLDSKQTLGDLFDSYDETKIHSNPGVRWDLFPGGEQEGRLRTVVDFFG----NVQS 298
+ +K+T L Y K H+N + G + V+ F G
Sbjct: 242 DSDVEDLTKET---LHKQYHLVKSHTNT---SHVMQYGNKSISTMKVMQFQGMKHRASSP 295
Query: 299 VEIEKA-DADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQEETRSAPAYEVKK 355
+ + D S L + + + + + + + R V+K
Sbjct: 296 ISLPPVTHLDLTPSPDVPLTILKRKLLRTNNMKESQVLVGQIQHLLDARHIIEKSVQK 353
>ref|NP_776526.1| legumain [Bos taurus]
dbj|BAB69947.1| legumain [Bos taurus]
Length = 433
Score = 511 bits (1303), Expect = e-143
Identities = 94/355 (26%), Positives = 154/355 (42%), Gaps = 21/355 (5%)
Query: 10 LPFLLSLLFAVFASAEH-TSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQIIL 68
L +L E +W V+VA S W+NYRH A+ Y+ V R GIPD QII+
Sbjct: 9 LSLVLGTGAVPLEDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIVHRNGIPDEQIIV 68
Query: 69 MLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRLL-TDRLDEDV 127
M+ DD+A + N PG V N D+Y + DY G +VT +NF+ +L D
Sbjct: 69 MMYDDIANSEDNPTPGIVI-NRPNGSDVY-QGVLKDYTGEDVTPKNFLAVLRGDAEAVKG 126
Query: 128 -PRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELLFM 186
K L S +V VY T HG L F + E++ DL + M+E K Y +++F
Sbjct: 127 VGSGKVLKSGPRDHVFVYFTDHGATGILVFPN-EDLHVKDLNETIRYMYEHKMYQKMVFY 185
Query: 187 IDTCQANTMYTHFYSP-NIIATGSSELDQSSYSHHADNDVGVAVIDRWTYYVLEFLENRV 245
I+ C++ +M H N+ AT ++ +SSY+ + D + D +Y + ++E+
Sbjct: 186 IEACESGSMMNHLPPDINVYATTAANPRESSYACYYDEQRSTFLGD---WYSVNWMEDSD 242
Query: 246 TSLDSKQTLGDLFDSYDETKIHSNPGVRWDLFPGGEQEGRLRTVVDFFG----NVQSVEI 301
+K+T L Y K H+N + G + ++ F G + +
Sbjct: 243 VEDLTKET---LHKQYQLVKSHTNT---SHVMQYGNKSISAMKLMQFQGLKHQASSPISL 296
Query: 302 E-KADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQEETRSAPAYEVKK 355
+ D S + L + + + + + + E R+ V+K
Sbjct: 297 PAVSRLDLTPSPEVPLSIMKRKLMSTNDLQESRRLVQKIDRHLEARNIIEKSVRK 351
>ref|NP_035305.1| legumain; protease, cysteine, 1; preprolegumain [Mus musculus]
sp|O89017|LGMN_MOUSE Legumain precursor (Asparaginyl endopeptidase) (Protease, cysteine
1)
emb|CAA04439.1| legumain [Mus musculus]
gb|AAF21659.1| preprolegumain; cysteine Protease [Mus musculus]
Length = 435
Score = 510 bits (1301), Expect = e-143
Identities = 94/358 (26%), Positives = 153/358 (42%), Gaps = 21/358 (5%)
Query: 7 LQALPFLLSLLFAVFASAEH-TSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQ 65
L +L + E +W V+VA S W+NYRH A+ Y+ + R GIPD Q
Sbjct: 8 LLSLVLGAGAVPVGVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQ 67
Query: 66 IILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRLL-TDRLD 124
II+M+ DD+A + N PG V N D+Y + DY G +VT ENF+ +L D
Sbjct: 68 IIVMMYDDIANSEENPTPGVVI-NRPNGTDVY-KGVLKDYTGEDVTPENFLAVLRGDAEA 125
Query: 125 EDV-PRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHEL 183
K L S +V +Y T HG L F + +++ DL M+E K Y ++
Sbjct: 126 VKGKGSGKVLKSGPRDHVFIYFTDHGATGILVFPN-DDLHVKDLNKTIRYMYEHKMYQKM 184
Query: 184 LFMIDTCQANTMYTHFYSP-NIIATGSSELDQSSYSHHADNDVGVAVIDRWTYYVLEFLE 242
+F I+ C++ +M H N+ AT ++ +SSY+ + D + G + D +Y + ++E
Sbjct: 185 VFYIEACESGSMMNHLPDDINVYATTAANPKESSYACYYDEERGTYLGD---WYSVNWME 241
Query: 243 NRVTSLDSKQTLGDLFDSYDETKIHSNPGVRWDLFPGGEQEGRLRTVVDFFG----NVQS 298
+ +K+T L Y K H+N + G + V+ F G
Sbjct: 242 DSDVEDLTKET---LHKQYHLVKSHTNT---SHVMQYGNKSISTMKVMQFQGMKHRASSP 295
Query: 299 VEIEKA-DADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQEETRSAPAYEVKK 355
+ + D S L + + + + + + Q + R V K
Sbjct: 296 ISLPPVTHLDLTPSPDVPLTILKRKLLRTNDVKESQNLIGQIQQFLDARHVIEKSVHK 353
>emb|CAD61872.1| unnamed protein product [Homo sapiens]
Length = 376
Score = 510 bits (1300), Expect = e-143
Identities = 90/361 (24%), Positives = 151/361 (40%), Gaps = 25/361 (6%)
Query: 10 LPFLLSLLFAVFASAEH-TSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQIIL 68
L L + E +W V+VA S W+NYRH A+ Y+ + R GIPD QI++
Sbjct: 9 LSVALGIGAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVV 68
Query: 69 MLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRLL-TDRLDEDV 127
M+ DD+A + N PG V N D+Y + DY G +VT +NF+ +L D
Sbjct: 69 MMYDDIAYSEDNPTPGIVI-NRPNGTDVY-QGVPKDYTGEDVTPQNFLAVLRGDAEAVKG 126
Query: 128 -PRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELLFM 186
K L S +V +Y T HG L F + E++ DL + M++ K Y +++F
Sbjct: 127 IGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYKHKMYRKMVFY 185
Query: 187 IDTCQANTMYTHFYSP-NIIATGSSELDQSSYSHHADNDVGVAVIDRWTYYVLEFLENRV 245
I+ C++ +M H N+ AT ++ +SSY+ + D + D +Y + ++E+
Sbjct: 186 IEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGD---WYSVNWMEDSD 242
Query: 246 TSLDSKQTLGDLFDSYDETKIHSNPGVRWDLFPGGEQEGRLRTVVDFFG----NVQSVEI 301
+K+T L Y K H+N + G + V+ F G V +
Sbjct: 243 VEDLTKET---LHKQYHLVKSHTNT---SHVMQYGNKTISTMKVMQFQGMKRKASSPVPL 296
Query: 302 EKA-DADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQEETRSAPAYEVKKRNVGP 360
D S L + + + + + + + Y ++ V
Sbjct: 297 PPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLD----YEYALRHLYVLV 352
Query: 361 A 361
Sbjct: 353 N 353
>emb|CAD61895.1| unnamed protein product [Homo sapiens]
Length = 372
Score = 509 bits (1299), Expect = e-143
Identities = 88/346 (25%), Positives = 148/346 (42%), Gaps = 21/346 (6%)
Query: 10 LPFLLSLLFAVFASAEH-TSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLGIPDSQIIL 68
L L + E +W V+VA S W+NYRH A+ Y+ + R GIPD QI++
Sbjct: 9 LSVALGIGAVPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVV 68
Query: 69 MLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRLL-TDRLDEDV 127
M+ DD+A + N PG V N D+Y + DY G +VT +NF+ +L D
Sbjct: 69 MMYDDIAYSEDNPTPGIVI-NRPNGTDVY-QGVPKDYTGEDVTPQNFLAVLRGDAEAVKG 126
Query: 128 -PRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRYHELLFM 186
K L S +V +Y T HG L F + E++ DL + M++ K Y +++F
Sbjct: 127 IGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN-EDLHVKDLNETIHYMYKHKMYRKMVFY 185
Query: 187 IDTCQANTMYTHFYSP-NIIATGSSELDQSSYSHHADNDVGVAVIDRWTYYVLEFLENRV 245
I+ C++ +M H N+ AT ++ +SSY+ + D + D +Y + ++E+
Sbjct: 186 IEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGD---WYSVNWMEDSD 242
Query: 246 TSLDSKQTLGDLFDSYDETKIHSNPGVRWDLFPGGEQEGRLRTVVDFFG----NVQSVEI 301
+K+T L Y K H+N + G + V+ F G V +
Sbjct: 243 VEDLTKET---LHKQYHLVKSHTNT---SHVMQYGNKTISTMKVMQFQGMKRKASSPVPL 296
Query: 302 EKA-DADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQEETR 346
D S L + + + + + + + +
Sbjct: 297 PPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLDDK 342
>gb|AAD04882.1| C13 endopeptidase NP1 precursor [Hordeum vulgare]
Length = 411
Score = 501 bits (1278), Expect = e-140
Identities = 62/327 (18%), Positives = 129/327 (38%), Gaps = 24/327 (7%)
Query: 48 NVLSLYRTVKRLGIPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRG 107
+V Y+ +K+ G+ D I++ + DD+A +P N PG V N + D+Y + DY G
Sbjct: 1 DVCHAYQILKKGGLKDENIVVFMYDDIANSPDNPRPGIVI-NHPKGKDVYH-GVPKDYTG 58
Query: 108 YEVTVENFIRL-LTDRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWD 166
+VT +NF + L ++ K + S ++ +Y T HG L + ++ A D
Sbjct: 59 DQVTAKNFYAVLLGNKTAVTGGSRKVVNSKPNDHIFIYYTDHGAAGLLCMPNPPDVYADD 118
Query: 167 LADAFGQMWEKKRYHELLFMIDTCQANTMYTHFYSP--NIIATGSSELDQSSYSHHA--- 221
Q K Y +++ ++ C++ +++ NI T ++ ++S++ +
Sbjct: 119 FIKVLRQKHASKSYSKMIIYVEACESGSIFEGLMPQDHNIYVTTAANAQENSWAAYCPTM 178
Query: 222 ----DNDVGVAVIDRWTYYVLEFLENRVTSLDSKQTLGDLFDSYDETKIHSNPGVRWDLF 277
+ + D Y + ++E+ T +++ Y+ K + P +
Sbjct: 179 EIPPPPEYDTCLGDA---YSVSWMEDSETQDLKNESIK---QQYEVVKERTAPL--SHVM 230
Query: 278 PGGEQEGRLRTVVDFFGNVQSVEIEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVAN 337
G++ + + F G + + SLK I Q + Y N
Sbjct: 231 EYGDKTFKEDMLFLFQGFDPAKSSIRNRPLPMPSLKG---AINQRDADILFMWRKYEKLN 287
Query: 338 STS-QQEETRSAPAYEVKKRNVGPARL 363
+ S +++ V R + +
Sbjct: 288 AGSEEKQRALREVKETVLHRKHLDSSI 314
>gb|EAA46796.1| hypothetical protein MG10490.4 [Magnaporthe grisea 70-15]
Length = 394
Score = 499 bits (1272), Expect = e-140
Identities = 256/395 (64%), Positives = 305/395 (76%), Gaps = 6/395 (1%)
Query: 1 MSPYFGLQALPFLLSLLFAVFASAEHTSNWAVLVATSRFWFNYRHLANVLSLYRTVKRLG 60
+S L L L A +AEHTSNWAVLV TSRFWFNYRHLANVLS+YRTVKRLG
Sbjct: 3 LSRLLQLPTLLAAALLAVAPPVAAEHTSNWAVLVCTSRFWFNYRHLANVLSMYRTVKRLG 62
Query: 61 IPDSQIILMLPDDMACNPRNAFPGTVYSNADRAVDLYGDNIEVDYRGYEVTVENFIRLLT 120
IPDSQIILMLPDDMACNPRNAFPGTVYSNA+RAVDLYGDNIEVDYRGYEVTVENFIRLLT
Sbjct: 63 IPDSQIILMLPDDMACNPRNAFPGTVYSNAERAVDLYGDNIEVDYRGYEVTVENFIRLLT 122
Query: 121 DRLDEDVPRSKRLGSDAGSNVLVYMTGHGGDQFLKFQDAEEIGAWDLADAFGQMWEKKRY 180
DR+ +++PRSKRL +D SN+LVYMTGHGG++FLKFQDAEEIGA+DLADAF QMWEKKRY
Sbjct: 123 DRVGDEMPRSKRLLTDDRSNILVYMTGHGGNEFLKFQDAEEIGAFDLADAFEQMWEKKRY 182
Query: 181 HELLFMIDTCQANTMYTHFYSPNIIATGSSELDQSSYSHHADNDVGVAVIDRWTYYVLEF 240
+E+LFMIDTCQANTM T YSPNIIATGSSELDQSSYSHHADNDVGVAVIDR+TYY L+F
Sbjct: 183 NEILFMIDTCQANTMLTKLYSPNIIATGSSELDQSSYSHHADNDVGVAVIDRYTYYNLDF 242
Query: 241 LENRVTSLDSKQTLGDLFDSYDETKIHSNPGVRWDLFPGGEQEGRLRTVVDFFGNVQSVE 300
LEN V ++SK+T+GDLFDSY KIHS+ G+R+DLFPGG R + + DFFGNVQ+VE
Sbjct: 243 LENEVKDINSKKTIGDLFDSYTFEKIHSHAGMRYDLFPGGADAARAKLLTDFFGNVQNVE 302
Query: 301 IEKADADEPGSLKEDLIEIAQLVEKWRTFSQDYAVANSTSQQEETRSAPAY---EVKKRN 357
+ DA+ L EDLI +++ + + ++ A ++ +E + +A + KR+
Sbjct: 303 V---DANVTTGLDEDLIALSKKIAALKAKAESEESAAASENRESSTTAAPKDNQSLPKRS 359
Query: 358 VGPARLTDETPWEKRLIGLSILGACAAVWFTGSTL 392
LTD+ W K++I + + CA +W S L
Sbjct: 360 QITKPLTDDNYWTKKIISATAISGCALLWGLSSYL 394
CPU time: 192.50 user secs. 4.66 sys. secs 197.16 total secs.
Database: nr
Posted date: Nov 22, 2003 1:30 PM
Number of letters in database: 504,497,942
Number of sequences in database: 1,543,518
Lambda K H
0.313 0.180 0.597
Gapped
Lambda K H
0.270 0.0630 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 561804776
Number of Sequences: 1543518
Number of extensions: 35046392
Number of successful extensions: 61494
Number of sequences better than 10.0: 119
Number of HSP's better than 10.0 without gapping: 103
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 60770
Number of HSP's gapped (non-prelim): 127
length of query: 397
length of database: 504,497,942
effective HSP length: 58
effective length of query: 339
effective length of database: 414,973,898
effective search space: 140676151422
effective search space used: 140676151422
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.0 bits)
S2: 77 (34.0 bits)