Sequences with E-value BETTER than threshold (0.002) 464 e-130
461 e-129
461 e-129
461 e-129
460 e-128
460 e-128
460 e-128
459 e-128
459 e-128
457 e-127
456 e-127
455 e-127
455 e-127
455 e-127
454 e-127
453 e-126
453 e-126
452 e-126
452 e-126
451 e-126
448 e-125
443 e-123
443 e-123
443 e-123
442 e-123
441 e-123
439 e-122
439 e-122
438 e-122
435 e-121
432 e-120
432 e-120
426 e-118
421 e-117
420 e-116
414 e-114
409 e-113
403 e-111
402 e-111
402 e-111
402 e-111
400 e-110
396 e-109
396 e-109
395 e-109
394 e-108
394 e-108
390 e-107
389 e-107
383 e-105
Alignments
>dbj|BAA84650.1| (AB025310) asparaginyl endopeptidase [Oryza sativa]
dbj|BAB85400.1| (AP003924) asparaginyl endopeptidase [Oryza sativa (japonica
cultivar-group)]
Length = 501
Score = 464 bits (1183), Expect = e-130
Identities = 50/300 (16%), Positives = 110/300 (36%), Gaps = 19/300 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S ++NYR + YQ +K + D+ I + + +D A N +N PGV
Sbjct: 66 WAVLIAGSNGYYNYRHQADVCHAYQIMKRGGLKDENIIVFMYDDIAHNPENPRPGVIINH 125
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+ +++ V D+ +VN+K V+ S + L + +F + H
Sbjct: 126 --PQGGDVYAGVPKDYTGKEVNVKNLFAVLLGNKTAVKGGSGKVLDSGPNDHIFIFYSDH 183
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M YL ++ + K+ Y+ ++C + ++FE + + +
Sbjct: 184 GGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLLPNGINVYA 243
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ S G C S ++ L + W++D ++ +L Q
Sbjct: 244 TTASNADESSWGTYCPGEYPSPPPEYDTCLGDLYSVAWMEDSDVHNLRTESLKQQYNLVK 303
Query: 252 YDYHKAN----SKLIINMENKQAQDIKAADFWK--PKQPM---LNQVILRNDQDILAQQD 302
+ ++ + + + P + L + + Q+D
Sbjct: 304 ERTSVQHTYYSGSHVMEYGSLELNAHHVFMYMGSNPANDNATFVEDNSLPSFSRAVNQRD 363
>sp|P49046|LEGU_CANEN Legumain precursor (Asparaginyl endopeptidase)
pir||JX0344 legumain (EC 3.4.22.34) precursor - jack bean
dbj|BAA06596.1| (D31787) asparaginyl endopeptidase (Legumain) [Canavalia
ensiformis]
Length = 475
Score = 461 bits (1176), Expect = e-129
Identities = 50/299 (16%), Positives = 123/299 (40%), Gaps = 18/299 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S + NYR + YQ L + + ++ I + + +D A N N PGV
Sbjct: 41 WAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVVFMYDDIAYNAMNPRPGVIINH 100
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+ P+++ V D+ DV + V+ ++ S + + + E ++ +F + H
Sbjct: 101 --PQGPDVYAGVPKDYTGEDVTPENLYAVILGDKSKVKGGSGKVINSNPEDRIFIFYSDH 158
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M + ++ + + K+ Y+E + ++C + ++FE + +++ +
Sbjct: 159 GGPGVLGMPNAPFVYAMDFIDVLKKKHASGGYKEMVIYIEACESGSIFEGIMPKDLNIYV 218
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C ++ +++ L + W++D E + T+ Q +
Sbjct: 219 TTASNAQENSFGTYCPGMNPPPPEEYVTCLGDLYSVSWMEDSETHNLKRETVQQQYQSVR 278
Query: 252 YDYHKAN----SKLIINMENKQAQDIKAADF--WKPKQPML--NQVILRNDQDILAQQD 302
+N ++ + K + + P + L +++ Q+D
Sbjct: 279 KRTSNSNSYRFGSHVMQYGDTNITAEKLYLYHGFDPATVNFPPHNGNLEAKMEVVNQRD 337
>emb|CAC18099.1| (AJ251453) putative legumain [Zea mays]
Length = 486
Score = 461 bits (1176), Expect = e-129
Identities = 49/300 (16%), Positives = 112/300 (37%), Gaps = 19/300 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S ++NYR + YQ +K+ + D+ I + + +D A + +N PGV
Sbjct: 51 WAVLIAGSSGYYNYRHQADICHAYQIMKKGGLKDENIIVFMYDDIAHSPENPRPGVIINH 110
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+ +++ V D+ DVN+ + V+ S + + + +F + H
Sbjct: 111 --PQGGDVYAGVPKDYTGRDVNVDNFFAVLLGNKTALRGGSGKVVDSGPNDHIFVFYSDH 168
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M YL ++ + K+ Y+ ++C + ++FE + ++ +
Sbjct: 169 GGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLLPNDINVYA 228
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ S G C S ++ L + W++D + +L Q K
Sbjct: 229 TTASNADESSWGTYCPGEFPSPPPEYDTCLGDLYSVAWMEDSDFHNLRTESLKQQYKLVK 288
Query: 252 YDYHK----ANSKLIINMENKQAQDIKAADFWK--PKQP---MLNQVILRNDQDILAQQD 302
+ ++ + + + P + L + + Q+D
Sbjct: 289 DRTAVHDTFSYGSHVMQYGALELNVQRLFSYIGTDPANDGNTFIEDNSLPSFSKAVNQRD 348
>emb|CAB64544.1| (AJ131718) legumain-like protease [Zea mays]
Length = 486
Score = 461 bits (1175), Expect = e-129
Identities = 49/300 (16%), Positives = 113/300 (37%), Gaps = 19/300 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S ++NYR + YQ +K+ + D+ I + + +D A + +N PGV
Sbjct: 51 WAVLIAGSSGYYNYRHQADICHAYQIMKKGGLKDENIIVFMYDDIAHSPENPRPGVIINH 110
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+ +++ V D+ DVN+ + V+ S + + + + +F + H
Sbjct: 111 --PQGGDVYAGVPKDYTGRDVNVDNFFAVLLGNKTALRGGSGKVVDSGPDDHIFVFYSDH 168
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M YL ++ + K+ Y+ ++C + ++FE + ++ +
Sbjct: 169 GGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLLPNDINVYA 228
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C S ++ L + W++D + +L Q K
Sbjct: 229 TTASNAEESSWGTYCPGEFPSPPPEYDTCLGDLYSVAWMEDSDFHNLRTESLKQQYKLVK 288
Query: 252 YDYHK----ANSKLIINMENKQAQDIKAADFWK--PKQP---MLNQVILRNDQDILAQQD 302
+ ++ + + P + L + + Q+D
Sbjct: 289 DRTAVHDTFSYGSHVMQYGALELNVQHLFSYIGTDPANDGNTFIEDNSLPSFSKAVNQRD 348
>sp|P49045|VPE_SOYBN VACUOLAR PROCESSING ENZYME PRECURSOR (VPE)
pir||T07132 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - soybean
dbj|BAA06030.1| (D28876) cysteine proteinase [Glycine max]
Length = 495
Score = 460 bits (1173), Expect = e-128
Identities = 50/299 (16%), Positives = 117/299 (38%), Gaps = 18/299 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S + NYR + YQ L + + ++ I + + +D A N N GV
Sbjct: 61 WAVLVAGSNGYGNYRHQADVCHAYQLLIKGGLKEENIVVFMYDDIATNELNPRHGVIINH 120
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+L+ V D+ ++V + V+ ++ S + + E ++ ++ + H
Sbjct: 121 --PEGEDLYAGVPKDYTGDNVTTENLFAVILGDKSKLKGGSGKVINSKPEDRIFIYYSDH 178
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M + YL + + K+ Y+E + ++C + ++FE + +++ +
Sbjct: 179 GGPGILGMPNMPYLYAMDFIDVLKKKHASGSYKEMVIYVEACESGSVFEGIMPKDLNIYV 238
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C + S ++ L + W++D E + ++ Q K
Sbjct: 239 TTASNAQENSWGTYCPGMDPSPPPEYITCLGDLYSVAWMEDSEAHNLKRESVKQQYKSVK 298
Query: 252 YDYHKAN----SKLIINMENKQAQDIKAADF--WKPKQPMLN--QVILRNDQDILAQQD 302
N ++ + K + + P L +++ Q+D
Sbjct: 299 QRTSNFNNYAMGSHVMQYGDTNITAEKLYLYQGFDPATVNFPPQNGRLETKMEVVNQRD 357
>sp|P49042|VPE_RICCO Vacuolar processing enzyme precursor (VPE)
pir||JQ2387 vacuolar processing enzyme (EC 3.4.22.-) precursor - castor bean
dbj|BAA04225.1| (D17401) precursor of vacuolar processing enzyme [Ricinus communis]
Length = 497
Score = 460 bits (1172), Expect = e-128
Identities = 54/299 (18%), Positives = 119/299 (39%), Gaps = 18/299 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S + NYR + YQ L++ + ++ I + + +D A N N PGV
Sbjct: 63 WAVLVAGSMGFGNYRHQADVCHAYQLLRKGGLKEENIIVFMYDDIAKNELNPRPGVIINH 122
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+ +++ V D+ V K V+ + S + + ++ ++ + H
Sbjct: 123 --PQGEDVYAGVPKDYTGEHVTAKNLYAVLLGDKSAVQGGSGKVVDSKPNDRIFLYYSDH 180
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M + YL + ++ K+ Y++ + ++C + ++FE + +++ +
Sbjct: 181 GGPGVLGMPNLPYLYAMDFIEVLKKKHAAGGYKKMVIYVEACESGSIFEGIMPKDVDIYV 240
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C + S +F L + W++D E K T+ Q
Sbjct: 241 TTASNAQESSWGTYCPGMEPSPPPEFTTCLGDLYSVAWMEDSESHNLKKETVKQQYSSVK 300
Query: 252 YDYHKAN----SKLIINMENKQAQDIKAADF--WKPKQPML--NQVILRNDQDILAQQD 302
N ++ N+ + K F + P N L +++ Q+D
Sbjct: 301 ARTSNYNTYAAGSHVMQYGNQSIKADKLYLFQGFDPASVNFPPNNAHLNAPMEVVNQRD 359
>emb|CAC18100.1| (AJ251454) putative legumain [Zea mays]
Length = 485
Score = 460 bits (1172), Expect = e-128
Identities = 47/300 (15%), Positives = 112/300 (36%), Gaps = 19/300 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S ++NYR + YQ +K+ + D+ I + + +D A + +N PGV
Sbjct: 50 WAVLIAGSNGYYNYRHQADICHAYQIMKKGGLKDENIVVFMYDDIAHSPENPRPGVIINH 109
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+ +++ V D+ +VN+ + V+ S + + + +F + H
Sbjct: 110 --PQGGDVYAGVPKDYTGREVNVDNFFAVLLGNKTALRGGSGKVVDSGPNDHIFVFYSDH 167
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M YL ++ + K+ Y+ ++C + ++FE + ++ +
Sbjct: 168 GGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLLPNDINVYA 227
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C S ++ L + W++D + +L Q
Sbjct: 228 TTASNAEESSWGTYCPGEFPSPPPEYDTCLGDLYSVAWMEDSDFHNLRTESLKQQYNLVK 287
Query: 252 YDYHK----ANSKLIINMENKQAQDIKAADFWK--PKQP---MLNQVILRNDQDILAQQD 302
+ ++ + + + P + L + + Q+D
Sbjct: 288 DRTAVQDTFSYGSHVMQYGSLELNVKHLFSYIGTNPANDDNTSIEDNSLPSFSKAVNQRD 347
>dbj|BAA76744.1| (D89971) asparaginyl endopeptidase (VmPE-1) [Vigna mungo]
Length = 483
Score = 459 bits (1171), Expect = e-128
Identities = 47/299 (15%), Positives = 113/299 (37%), Gaps = 18/299 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S ++NYR + YQ L + + ++ I + + +D A N +N PGV
Sbjct: 49 WAVLIAGSNGYWNYRHQSDVCHAYQLLTKGGLKEENIVVFMYDDIAFNEENPRPGVIIN- 107
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+++K V D+ DV + + + + T S + + + ++ + H
Sbjct: 108 -SPHGNDVYKGVPKDYVGEDVTVNNFFAAILGNKSALTGGSGKVVNSGPNDHIFIYYSDH 166
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M + Y+ ++ ++ K+ Y+ + C + ++F + + + +
Sbjct: 167 GGPGVLGMPTSPYMYASDLIEVLKKKHASGTYKSLAFYLEGCESGSIFGGLLPEGLNIYA 226
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C + S ++ L + W++D ++ TL Q +
Sbjct: 227 TTAANAEESSWGTYCPGDNPSPPPEYETCLGDLYSVAWMEDSDIHNLRTETLHQQFELVK 286
Query: 252 YDYHK---ANSKLIINMENKQAQDIKAADFWK--PKQPML---NQVILRNDQDILAQQD 302
A ++ + + + P + L + Q+D
Sbjct: 287 QRTMNGNSAYGSHVMQYGDVGLSKNNVSLYLGTNPANDNFPFREKNSLVPPSKAVNQRD 345
>gb|AAD04883.1| (AF082347) C13 endopeptidase NP1 precursor [Zea mays]
Length = 485
Score = 459 bits (1169), Expect = e-128
Identities = 48/300 (16%), Positives = 112/300 (37%), Gaps = 19/300 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S ++NYR + YQ +K+ + D+ I + + +D A + +N PGV
Sbjct: 50 WAVLIAGSNGYYNYRHQADICHAYQIMKKGGLKDENIVVFMYDDIAHSPENPRPGVIINH 109
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+ +++ V D+ DVN+ + V+ S + + + +F + H
Sbjct: 110 --PQGGDVYAGVPKDYTGRDVNVDNFFAVLLGNKTALRGGSGKVVDSGPNDHIFVFYSDH 167
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M YL ++ + K+ Y+ ++C + ++FE + ++ +
Sbjct: 168 GGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLLPNDINVYA 227
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C S ++ L + W++D + +L Q
Sbjct: 228 TTASNAEESSWGTYCPGEFPSPPPEYDTCLGDLYSVAWMEDSDFHNLRTESLKQQYNLVK 287
Query: 252 YDYHK----ANSKLIINMENKQAQDIKAADFWK--PKQP---MLNQVILRNDQDILAQQD 302
+ ++ + + + P + L + + Q+D
Sbjct: 288 DRTAVQDTFSYGSHVMQYGSLELNVKHLFSYIGTNPANDDNTFIEDNSLPSLSKAVNQRD 347
>ref|NP_180165.1| (NM_128154) putative vacuolar processing enzyme [Arabidopsis
thaliana]
sp|P49047|VPEA_ARATH VACUOLAR PROCESSING ENZYME, ALPHA-ISOZYME PRECURSOR (ALPHA-VPE)
pir||T02629 vacuolar processing enzyme (EC 3.4.22.-) isozyme alpha precursor -
Arabidopsis thaliana
gb|AAC31241.1| (AC004747) vacuolar processing enzyme, alpha-isozyme precursor
(alpha-VPE) [Arabidopsis thaliana]
dbj|BAA09614.2| (D61393) alpha-VPE [Arabidopsis thaliana]
gb|AAL24163.1| (AY058055) At2g25940/F17H15.3 [Arabidopsis thaliana]
Length = 478
Score = 457 bits (1164), Expect = e-127
Identities = 52/299 (17%), Positives = 116/299 (38%), Gaps = 18/299 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S ++NYR + YQ LK+ + ++ I + + +D A N +N PGV
Sbjct: 45 WAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRPGVIINS 104
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+++ V D+ ++VN+ + V+ S + + + ++ + H
Sbjct: 105 PN--GEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIYYSDH 162
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M + L ++ + K+ Y+ ++C + ++FE + + + +
Sbjct: 163 GGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGLNIYA 222
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C S ++ L + W++D E TL +Q +
Sbjct: 223 TTASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQYELVK 282
Query: 252 YDYH---KANSKLIINMENKQAQDIKAADFWK--PKQPML---NQVILRNDQDILAQQD 302
K+ ++ + K F P N+ +R + Q+D
Sbjct: 283 KRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENFTFVNENSIRPPSRVTNQRD 341
>emb|CAB64545.1| (AJ131719) legumain-like protease [Zea mays]
Length = 481
Score = 456 bits (1162), Expect = e-127
Identities = 48/300 (16%), Positives = 111/300 (37%), Gaps = 19/300 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S ++NYR + YQ +K+ + D+ I + + +D A + +N PGV
Sbjct: 46 WAVLIAGSNGYYNYRHQADICHAYQIMKKGGLKDENIVVFMYDDIAHSPENPRPGVIINH 105
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+ +++ V D+ DVN+ + V+ S + + + +F + H
Sbjct: 106 --PQGGDVYAGVPKDYTGRDVNVDNFFAVLLGNKTALRGGSGKVVDSGPNDHISVFYSDH 163
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M YL ++ + K+ Y+ ++C + ++FE + ++ +
Sbjct: 164 GGPGVLGMPTYPYLYGDDLVDVLKKKHAAGTYKSLVFYLEACESGSIFEGLLPNDINVYA 223
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C S ++ L + W++D + +L Q
Sbjct: 224 TTASNAEESSWGTYCPGEFPSPPPEYDTCLGDLYSVAWMEDSDFHNLRTESLKQQYNLVK 283
Query: 252 YDYHK----ANSKLIINMENKQAQDIKAADFWK--PKQP---MLNQVILRNDQDILAQQD 302
+ ++ + + P + L + + Q+D
Sbjct: 284 DRTAVQDTFSYGSHVMQYGSLGLNVKHLFSYIGTNPANDDNTFIEDNSLPSFSKAVNQRD 343
>pir||T12043 probable legumain (EC 3.4.22.34) precursor - kidney bean
emb|CAB17078.1| (Z99956) asparagine-specific endopeptidase precursor [Phaseolus
vulgaris]
Length = 484
Score = 455 bits (1161), Expect = e-127
Identities = 50/299 (16%), Positives = 110/299 (36%), Gaps = 18/299 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
I+ + S ++NYR + YQ L++ + D+ I + + +D A N +N GV
Sbjct: 50 WAILFAGSSGYWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNSENPRRGVIINS 109
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
++K V D+ DV + + ++ T S + + + +F + H
Sbjct: 110 PN--GDEVYKGVPKDYTGEDVTAHNFYAALLGDKSKLTGGSGKVVNSGPNDHIFIFYSDH 167
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G Y+ ++ ++ K+ Y+ ++C + ++FE + +++ +
Sbjct: 168 GGPGVLGSPAGPYIYASDLNEVLKKKHASGTYKNLVFYLEACESGSIFEGLLPEDINVYA 227
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ S G C S ++ L + W++D + TL Q K
Sbjct: 228 TTASNADESSWGTYCPGEDPSPPPEYSTCLGDLYSVAWMEDSDRHNLRTETLHQQYKLVK 287
Query: 252 YDYHKA---NSKLIINMENKQAQDIKAADFWK--PKQPML---NQVILRNDQDILAQQD 302
++ + + P L ++ L + + Q+D
Sbjct: 288 ERTISGGLYYGSHVMQYGDVGLSKDILFHYLGTDPANENLTFVDENSLWSSSKAVNQRD 346
>emb|CAB16318.1| (Z99174) cysteine proteinase precursor [Vicia narbonensis]
Length = 488
Score = 455 bits (1161), Expect = e-127
Identities = 52/299 (17%), Positives = 122/299 (40%), Gaps = 18/299 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S + NYR + YQ L + + ++ I + + +D A N N PGV
Sbjct: 54 WAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVVFMYDDIAYNEMNPRPGVIINH 113
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+ PN++ V D+ + V + + V+ ++ S + + E ++ ++ + H
Sbjct: 114 --PQGPNVYDGVPKDYNGDFVTAENFYAVILGDKSKVRGGSGKVINSKAEDRIFIYCSDH 171
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M + Y+ + + K+ Y++ + ++C + ++FE + +++ +
Sbjct: 172 GGPGVLGMPNMPYVYAMDFIDVLKKKHASGGYKKMVIYVEACESGSIFEGIMPKDINVYV 231
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C + + ++ L + W++D E + T+ Q
Sbjct: 232 TTASNAQENSWGTYCPGVDPAPPPEYITCLGDLYSVAWMEDSETHNLKRETVKQQYMSVR 291
Query: 252 YDYHKAN----SKLIINMENKQAQDIKAADF--WKPKQ--PMLNQVILRNDQDILAQQD 302
++ + D K + + P P N IL +++ Q+D
Sbjct: 292 ERTSNYKNYPLGSHVMQYGDTNITDEKLYLYHGFDPADGEPSSNNDILEAKMEVVNQRD 350
>gb|AAF69014.1|AF260827_1 (AF260827) cysteine protease [Ipomoea batatas]
Length = 492
Score = 455 bits (1159), Expect = e-127
Identities = 48/301 (15%), Positives = 110/301 (35%), Gaps = 20/301 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S ++NYR + YQ LK + D+ I + + +D A N +N G+
Sbjct: 56 WAVLIAGSNGYWNYRHQADICHAYQILKAGGLKDENIVVFMYDDIAYNEENPRKGIIIN- 114
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+++ V D+ +DV + V+ + S + + + ++ + H
Sbjct: 115 -SPHGEDVYHGVPKDYTGDDVTANNLLAVILGDKSAVKGGSGKVVDSGPNDHIFIYYSDH 173
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M + YL E+ K+ Y+ ++C + ++FE + +++ +
Sbjct: 174 GGPGVLGMPTSPYLYADELNAALKKKHAAGAYKSLVFYLEACESGSIFEGILPKDINIYA 233
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ S G C S ++ L + W++D ++ +L Q
Sbjct: 234 TTASNAIESSWGTYCPGEYPSPPPEYETCLGDLYSIAWMEDSDIHNLRTESLKQQYNLVK 293
Query: 252 YDYHK---ANSKLIINMENKQAQDIKAADFWK--PKQPML-----NQVILRNDQDILAQQ 301
A ++ + + + P + L + + Q+
Sbjct: 294 DRTLNGNTAYGSHVMQYGDLELNADSLFMYMGTNPANENFTFVDEKSLKLSAPRRAVNQR 353
Query: 302 D 302
D
Sbjct: 354 D 354
>gb|AAF89679.1| (AF169973) asparaginyl endopeptidase [Sesamum indicum]
Length = 489
Score = 454 bits (1157), Expect = e-127
Identities = 52/299 (17%), Positives = 118/299 (39%), Gaps = 18/299 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S + NYR + YQ LK+ + D+ I + + +D A N N GV
Sbjct: 55 WAVLVAGSNGFGNYRHQADVCHAYQILKKGGLRDENIIVFMYDDIAMNELNPRKGVIINH 114
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+++ V D+ V + V+ + S + + ++ ++ + H
Sbjct: 115 --PTGGDVYAGVPKDYTGEQVTAENLYAVILGDKSAIKGGSGKVVDSKPNDRIFIYYSDH 172
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M + YL + ++ K+ Y+E + ++C + ++FE + ++ +
Sbjct: 173 GGPGVLGMPNMPYLYANDFIEVLKKKHASGTYKEMVIYVEACESGSVFEGLMPDDLDIYV 232
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C + ++ L + W++D E + T+ Q ++
Sbjct: 233 TTASNAEESSWGTYCPGMDPPPPPEYITCLGDLYSVAWMEDSESHNLKRETVEQQYQQVK 292
Query: 252 YDYHKAN----SKLIINMENKQAQDIKAADF--WKPKQPMLNQ--VILRNDQDILAQQD 302
N ++ NK + K + + P + L+ D++ Q+D
Sbjct: 293 ERTSNFNTYNAGSHVMEYGNKSIKSEKLYLYQGFDPATENMPPSENHLKPHMDVVNQRD 351
>gb|AAK15049.1|AF238384_1 (AF238384) asparaginyl endopeptidase [Vigna radiata]
Length = 483
Score = 453 bits (1156), Expect = e-126
Identities = 48/298 (16%), Positives = 114/298 (38%), Gaps = 17/298 (5%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
I+ + S ++NYR + YQ L++ + ++ I + + +D A N N PGV +
Sbjct: 50 WAILFAGSNGYWNYRHQADICHAYQILRKGGLKEENIIVFMYDDIAFNWDNPRPGVIINK 109
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
++++ V D+ D + + + T S + + + ++ +F + H
Sbjct: 110 --PDGDDVYEGVPKDYTGEDATAHNFYSALLGDKSALTGGSGKVVNSGPDDRIFIFYSDH 167
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G Y+ ++ ++ K+ Y+ ++C A ++FE + +++ +
Sbjct: 168 GGPGVLGTPAGPYIYASDLVEVLKKKHASGTYKNLVFYLEACEAGSIFEGLLPEDINIYA 227
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C S ++ L + W++D + +L Q K
Sbjct: 228 TTASNAEESSWGTYCPGEYPSPPPEYSTCLGDLYSVAWMEDSDRHNLRTESLHQQYKVVK 287
Query: 252 YDYHKA--NSKLIINMENKQAQDIKAADFWK--PKQPML---NQVILRNDQDILAQQD 302
++ + + + P L ++ L + + Q+D
Sbjct: 288 DRTLSGGWYGSHVMQYGDVEFSKDTLFLYLGTDPANDNLTFVDENSLWSSSTAVNQRD 345
>pir||T12044 probable legumain (EC 3.4.22.34) precursor - kidney bean
emb|CAB17079.1| (Z99957) legumain-like proteinase precursor [Phaseolus vulgaris]
Length = 493
Score = 453 bits (1154), Expect = e-126
Identities = 49/299 (16%), Positives = 120/299 (39%), Gaps = 18/299 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S + NYR + YQ L + + ++ I + + +D A + N PGV
Sbjct: 59 WAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVVFMYDDIATHELNPRPGVIIN- 117
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+ P+++ V D+ V + V+ ++ S + + E ++ ++ + H
Sbjct: 118 -NPQGPDVYAGVPKDYTGESVTSHNFFAVLLGDKSKVKGGSGKVINSKPEDRIFVYYSDH 176
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M + YL + + K+ Y+E + ++C + ++FE + +++ +
Sbjct: 177 GGPGVLGMPNMPYLYAMDFIDVLKKKHASGGYKEMVIYVEACESGSIFEGIMPKDLNIYV 236
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C + ++ L + W++D E K ++ Q +
Sbjct: 237 TTASNAQENSWGTYCPGMYPPPPPEYITCLGDLYSVAWMEDSESHNLKKESVEQQYQSVK 296
Query: 252 YDYHK----ANSKLIINMENKQAQDIKAADF--WKPKQPML--NQVILRNDQDILAQQD 302
A ++ + K + + P + L++ +++ Q+D
Sbjct: 297 QRTSNFEAYAMGSHVMQYGDANMTAEKLYLYHGFDPATVNFPPHNGRLKSKMEVVNQRD 355
>pir||S60049 vacuolar processing enzyme (EC 3.4.22.-) isozyme alpha precursor -
Arabidopsis thaliana
Length = 478
Score = 452 bits (1152), Expect = e-126
Identities = 52/299 (17%), Positives = 118/299 (39%), Gaps = 18/299 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S ++NYR + YQ+LK+ + ++ I +++ +D A N +N PGV
Sbjct: 45 WAVLVAGSSGYWNYRHQADVCHAYQRLKKGGVKEENIVVLMYDDIAENEENPRPGVIINS 104
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+++ V D+ ++VN+ + V+ S + + + ++ + H
Sbjct: 105 PN--GEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIYYSDH 162
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M + L ++ + K+ Y+ ++C + ++FE + + + +
Sbjct: 163 GGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGLNIYA 222
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C S ++ L + W++D E TL +Q +
Sbjct: 223 TTASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQYELVK 282
Query: 252 YDYH---KANSKLIINMENKQAQDIKAADFWK--PKQPML---NQVILRNDQDILAQQD 302
K+ ++ + K F P N+ +R + Q+D
Sbjct: 283 KRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENFTFVNENSIRPPSRVTNQRD 341
>dbj|BAA76745.1| (D89972) asparaginyl endopeptidase (VmPE-1A) [Vigna mungo]
Length = 482
Score = 452 bits (1151), Expect = e-126
Identities = 48/298 (16%), Positives = 115/298 (38%), Gaps = 17/298 (5%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
I+ + S ++NYR + YQ L++ + ++ I + + +D A N N PGV +
Sbjct: 49 WAILFAGSNGYWNYRHQADICHAYQILRKGGLKEENIIVFMYDDIAFNWDNPRPGVIINK 108
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
++++ V D+ D + + + T S + ++ + ++ +F + H
Sbjct: 109 --PDGDDVYEGVPKDYTGEDATAHNFYSALLGDKSALTGGSGKVVSSGPDDRIFIFYSDH 166
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G Y+ ++ ++ K+ Y+ ++C A ++FE + +++ +
Sbjct: 167 GGPGVLGTPAGPYIYASDLVEVLKKKHASGTYKNLVFYLEACEAGSIFEGLLPEDINIYA 226
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C S ++ L + W++D + +L Q K
Sbjct: 227 TTASNAEESSWGTYCPGEYPSPPPEYSTCLGDLYSVAWMEDSDRHNLRTESLHQQYKVVK 286
Query: 252 YDYHKA--NSKLIINMENKQAQDIKAADFWK--PKQPML---NQVILRNDQDILAQQD 302
++ + + + P L ++ L + + Q+D
Sbjct: 287 DRTLSGGWYGSHVMQYGDVEFSKDALFLYLGTDPANDNLTFVDENSLWSSSTAVNQRD 344
>gb|AAF89646.1|AF169019_1 (AF169019) seed maturation protein PM40 [Glycine max]
Length = 496
Score = 451 bits (1149), Expect = e-126
Identities = 47/300 (15%), Positives = 116/300 (38%), Gaps = 19/300 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S + NYR + YQ L + + ++ I + + +D A + N PGV
Sbjct: 61 WAVLVAGSNGYGNYRHQADVCHAYQLLIKGGLKEENIVVFMYDDIATDELNPRPGVIINH 120
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+++ V D+ +V + V+ N+ S + + E ++ ++ + H
Sbjct: 121 --PEGQDVYAGVPKDYTGENVTAQNLFAVILGDKNKVKGGSGKVINSKPEDRIFIYYSDH 178
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAIT-LFETVKAQNMILL 197
GG G M + YL + ++ K+ Y++ + ++C + + + + +++ +
Sbjct: 179 GGPGVLGMPNMPYLYAMDFIEVLKKKHASGGYKKMVIYVEACESGNHVLKGIMPKDLQIY 238
Query: 198 GSSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKY 250
+++ + S G C + S ++ L + W++D E + ++ Q K
Sbjct: 239 VTTASNAQENSWGTYCPGMDPSPPPEYITCLGDLYSVAWMEDSETHNLKRESVKQQYKSV 298
Query: 251 NYDYHKAN----SKLIINMENKQAQDIKAADF--WKPKQPMLN--QVILRNDQDILAQQD 302
N ++ + K + + P L +++ Q+D
Sbjct: 299 KQRTSNFNNYAMGSHVMQYGDTNITAEKLYLYQGFDPAAVNFPPQNGRLETKMEVVNQRD 358
>sp|P49043|VPE_CITSI VACUOLAR PROCESSING ENZYME PRECURSOR (VPE)
pir||S51117 cysteine proteinase (EC 3.4.22.-) precursor [similarity] - sweet
orange
emb|CAA87720.1| (Z47793) cystein proteinase (by similarity) [Citrus sinensis]
prf||2208463A vascular processing protease [Citrus sinensis]
Length = 494
Score = 448 bits (1143), Expect = e-125
Identities = 52/298 (17%), Positives = 117/298 (38%), Gaps = 17/298 (5%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++L+ S ++NYR + YQ L++ + D+ I + + +D A N +N PGV
Sbjct: 61 WAVLLAGSNGFWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNEENPRPGVIINH 120
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+++K V D+ DV ++ + V+ T S + + + +F + H
Sbjct: 121 --PHGDDVYKGVPKDYTGEDVTVEKFFAVVLGNKTALTGGSGKVVDSGPNDHIFIFYSDH 178
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M + Y+ E+ + K+ Y+ ++C + ++FE + + + +
Sbjct: 179 GGPGVLGMPTSRYIYADELIDVLKKKHASGNYKSLVFYLEACESGSIFEGLLLEGLNIYA 238
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C ++ L + W++D ++ TL Q +
Sbjct: 239 TTASNAEESSWGTYCPGEIPGPPPEYSTCLGDLYSIAWMEDSDIHNLRTETLHQQYELVK 298
Query: 252 YDY--HKANSKLIINMENKQAQDIKAADFWK--PKQPM---LNQVILRNDQDILAQQD 302
+ + ++ + + P +++ LR + Q+D
Sbjct: 299 TRTASYNSYGSHVMQYGDIGLSKNNLFTYLGTNPANDNYTFVDENSLRPASKAVNQRD 356
>ref|XP_039644.1| (XM_039644) phosphatidylinositol glycan, class K [Homo sapiens]
gb|AAB81597.1| (AF022913) GPI transamidase [Homo sapiens]
gb|AAH20737.1|AAH20737 (BC020737) Unknown (protein for MGC:22559) [Homo sapiens]
Length = 395
Score = 443 bits (1130), Expect = e-123
Identities = 68/283 (24%), Positives = 138/283 (48%), Gaps = 4/283 (1%)
Query: 19 NQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACA 78
N +++ TS+FWFNYR N+L +Y+ +K I D I LM+ +D ACN +N P +
Sbjct: 45 NWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVLMLADDMACNPRNPKPATVFS 104
Query: 79 QDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQSRRLTLSKEQKLLMFMNGH 138
+V D++ +V ++ ++ V+ + TP+S+RL +L++M GH
Sbjct: 105 HKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIPPSTPRSKRLLSDDRSNILIYMTGH 164
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG+G+ K QD+ + + E+ ++M + Y E I D+C +++E + N++ L
Sbjct: 165 GGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGASMYERFYSPNIMALA 224
Query: 199 SSSLGEKAYSHGRCSILSISKTDKFSLTTHYWLKDELDKKPKLTLLDQMKKYNYDYHKAN 258
SS +GE + SH + + D+++ +L+ E++ + + D + +
Sbjct: 225 SSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLE-EINPASQTNMNDLFQVCPKSLCVST 283
Query: 259 SKLIINMENKQAQDIKAADFWKPKQP---MLNQVILRNDQDIL 298
++ + +++ DF+ + + L+ D +I+
Sbjct: 284 PGHRTDLFQRDPKNVLITDFFGSVRKVEITTETIKLQQDSEIM 326
>emb|CAA68871.1| (Y07596) gpi8 [Homo sapiens]
Length = 396
Score = 443 bits (1130), Expect = e-123
Identities = 68/283 (24%), Positives = 138/283 (48%), Gaps = 4/283 (1%)
Query: 19 NQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACA 78
N +++ TS+FWFNYR N+L +Y+ +K I D I LM+ +D ACN +N P +
Sbjct: 46 NWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVLMLADDMACNPRNPKPATVFS 105
Query: 79 QDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQSRRLTLSKEQKLLMFMNGH 138
+V D++ +V ++ ++ V+ + TP+S+RL +L++M GH
Sbjct: 106 HKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIPPSTPRSKRLLSDDRSNILIYMTGH 165
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG+G+ K QD+ + + E+ ++M + Y E I D+C +++E + N++ L
Sbjct: 166 GGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGASMYERFYSPNIMALA 225
Query: 199 SSSLGEKAYSHGRCSILSISKTDKFSLTTHYWLKDELDKKPKLTLLDQMKKYNYDYHKAN 258
SS +GE + SH + + D+++ +L+ E++ + + D + +
Sbjct: 226 SSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLE-EINPASQTNMNDLFQVCPKSLCVST 284
Query: 259 SKLIINMENKQAQDIKAADFWKPKQP---MLNQVILRNDQDIL 298
++ + +++ DF+ + + L+ D +I+
Sbjct: 285 PGHRTDLFQRDPKNVLITDFFGSVRKVEITTETIKLQQDSEIM 327
>ref|NP_176458.1| (NM_104948) beta-VPE [Arabidopsis thaliana]
gb|AAK56243.1|AF367254_1 (AF367254) At1g62710/F23N19_8 [Arabidopsis thaliana]
gb|AAL15381.1| (AY059156) At1g62710/F23N19_8 [Arabidopsis thaliana]
Length = 486
Score = 443 bits (1129), Expect = e-123
Identities = 48/299 (16%), Positives = 116/299 (38%), Gaps = 18/299 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S + NYR + YQ L++ + ++ I +++ +D A + N PG
Sbjct: 52 WAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVVLMYDDIANHPLNPRPGTLINH 111
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+++ V D+ + V + V+ S + + + ++ H
Sbjct: 112 --PDGDDVYAGVPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPNDHIFVYYADH 169
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M +T ++ + + K+ Y+E + ++C + ++FE + +++ +
Sbjct: 170 GGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACESGSIFEGIMPKDLNIYV 229
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S+G C ++ S ++ L + W++D E K T+ Q
Sbjct: 230 TTASNAQESSYGTYCPGMNPSPPSEYITCLGDLYSVAWMEDSETHNLKKETIKQQYHTVK 289
Query: 252 YDYHK----ANSKLIINMENKQAQDIKAADF--WKPKQPMLNQVILRNDQD--ILAQQD 302
+ ++ N + K + + P L L ++ Q+D
Sbjct: 290 MRTSNYNTYSGGSHVMEYGNNSIKSEKLYLYQGFDPATVNLPLNELPVKSKIGVVNQRD 348
>pir||T10944 cysteine proteinase (EC 3.4.22.-) precursor - spring vetch
emb|CAA07639.1| (AJ007743) cysteine proteinase precursor [Vicia sativa]
Length = 503
Score = 442 bits (1126), Expect = e-123
Identities = 51/300 (17%), Positives = 120/300 (40%), Gaps = 19/300 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S + NYR + YQ L + + ++ I + + +D A + N PGV
Sbjct: 68 WAVLVAGSNGYGNYRHQADVCHAYQLLIKGGVKEENIVVFMYDDIAYSEFNPRPGVIINH 127
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+ PN++ V D+ + V V+ ++ S + + E ++ ++ + H
Sbjct: 128 --PQGPNVYDGVPKDYTGDFVTADNLYAVILGDKSKVRGGSGKVINSKAEDRIFIYYSDH 185
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M + Y+ + + K+ + YQ+ + ++C + ++F+ + +++ +
Sbjct: 186 GGPGVLGMPNMPYVYAMDFIDVLKKKHASRGYQQMVIYVEACESGSVFQGIMPKDIDVYV 245
Query: 199 SSSLGEKAYSHGRC--SILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKY 250
+++ + S G + + ++ L + W++D E + TL Q
Sbjct: 246 TTASNAEESSWGTYLVPGVYPASPPEYITCLGDLYSVAWMEDSETHNLKRETLKQQFASV 305
Query: 251 NYDYHKAN----SKLIINMENKQAQDIKAADF--WKPKQPML--NQVILRNDQDILAQQD 302
N + + D K + + P L N L + +++ Q+D
Sbjct: 306 KERTLNNNNYGLGSHVTEYGDTNITDEKLYLYHGFDPASVNLPPNNGRLESKMEVVNQRD 365
>pir||S60050 vacuolar processing enzyme (EC 3.4.22.-) isozyme beta precursor -
Arabidopsis thaliana
dbj|BAA09615.1| (D61394) beta-VPE [Arabidopsis thaliana]
Length = 484
Score = 441 bits (1125), Expect = e-123
Identities = 50/298 (16%), Positives = 120/298 (39%), Gaps = 18/298 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S + NYR + YQ L++ + ++ I +++ +D A + N PG
Sbjct: 52 WAVLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVVLMYDDIANHPLNPRPGTLINH 111
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+++ V D+ + V + V+ S + + + ++ H
Sbjct: 112 --PDGDDVYAGVPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPNDHIFVYYADH 169
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M +T ++ + + K+ Y+E + ++C + ++FE + +++ +
Sbjct: 170 GGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACESGSIFEGIMPKDLNIYV 229
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQM---K 248
+++ + S+G C ++ S ++ L + W++D E K T+ Q +
Sbjct: 230 TTASNAQESSYGTYCPGMNPSPPSEYITCLGDLYSVAWMEDSETHNLKKETIKQQYHTMR 289
Query: 249 KYNYDYHKANSKLIINMENKQAQDIKAADFWK--PKQPMLNQVILRNDQD--ILAQQD 302
NY+ + + ++ N + K + P L L ++ Q+D
Sbjct: 290 TSNYNTY-SGGSHVMEYGNNSIKSEKLYLYQGFEPATVNLPLNELPAKSKIGVVNQRD 346
>sp|P49044|VPE_VICSA VACUOLAR PROCESSING ENZYME PRECURSOR (VPE) (PROTEINASE B)
pir||S49175 legumain (EC 3.4.22.34) precursor [similarity] - spring vetch
pir||S68984 cysteine proteinase precursor - spring vetch
emb|CAA84383.1| (Z34899) cysteine proteinase [Vicia sativa]
Length = 493
Score = 439 bits (1120), Expect = e-122
Identities = 49/301 (16%), Positives = 110/301 (36%), Gaps = 20/301 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
I+L+ S ++NYR + YQ L++ ++ I + + +D A N +N PGV +
Sbjct: 57 WAILLAGSNGYWNYRHQSDVCHAYQLLRKGGSKEENIIVFMYDDIASNEENPRPGVIINK 116
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+++ V D+ +V+ + + + T S + + + ++ H
Sbjct: 117 --PDGDDVYAGVPKDYTGAEVHADNFYAALLGNKSALTGGSGKVVDSGPNDHIFVYYTDH 174
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M YL ++ ++ K+ Y+ ++C + ++FE + ++ +
Sbjct: 175 GGPGVLGMPVGPYLYASDLNEVLKKKHASGTYKSLVFYLEACESGSIFEGLLPDDLNIYA 234
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ + S G C ++ L + W++D E+ +L Q K
Sbjct: 235 TTASNAEESSWGYYCPGDKPPPPPEYSTCLGDLYSIAWMEDSEVHNLQTESLQQQYKLVK 294
Query: 252 YDYHKA-NSKLIINMENKQAQDIKAADFWKP---------KQPMLNQVILRNDQDILAQQ 301
++ + + N + LR + Q+
Sbjct: 295 NRTISEPYGSHVMEYGDIGLSKNDLYQYLGTNPANDNNSFVDETENSLKLRTPSAAVNQR 354
Query: 302 D 302
D
Sbjct: 355 D 355
>ref|NP_195020.1| (NM_119448) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
pir||T05302 vacuolar processing enzyme (EC 3.4.22.-) isozyme gamma precursor -
Arabidopsis thaliana
emb|CAA21203.1| (AL031804) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
emb|CAB80011.1| (AL161582) gamma-VPE (vacuolar processing enzyme) [Arabidopsis
thaliana]
gb|AAK43975.1|AF370160_1 (AF370160) putative gamma-VPE protein [Arabidopsis thaliana]
gb|AAL11612.1|AF424619_1 (AF424619) AT4g32940/F26P21_60 [Arabidopsis thaliana]
gb|AAL15210.1| (AY059104) putative gamma-VPE protein [Arabidopsis thaliana]
Length = 494
Score = 439 bits (1118), Expect = e-122
Identities = 49/299 (16%), Positives = 113/299 (37%), Gaps = 18/299 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S ++NYR + YQ L++ + ++ I + + +D A N +N PG
Sbjct: 60 WAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGTIIN- 118
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
++++ V D+ +DVN+ V+ S + + + +F + H
Sbjct: 119 -SPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYSDH 177
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M + YL ++ + K+ L Y+ ++C + ++FE + + + +
Sbjct: 178 GGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNIYA 237
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKDE-LDKKPKLTLLDQMKKYN 251
+++ + S G C S ++ L + W++D + TL Q +
Sbjct: 238 TTASNAEESSWGTYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMHNLQTETLHQQYELVK 297
Query: 252 YDYHK---ANSKLIINMENKQAQDIKAADFWK--PKQPML---NQVILRNDQDILAQQD 302
+ ++ + + P + L+ + Q+D
Sbjct: 298 RRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNFTFADANSLKPPSRVTNQRD 356
>sp|Q39119|VPEG_ARATH VACUOLAR PROCESSING ENZYME, GAMMA-ISOZYME PRECURSOR (GAMMA-VPE)
dbj|BAA18924.1| (D61395) gamma-VPE [Arabidopsis thaliana]
Length = 490
Score = 438 bits (1117), Expect = e-122
Identities = 49/299 (16%), Positives = 113/299 (37%), Gaps = 18/299 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S ++NYR + YQ L++ + ++ I + + +D A N +N PG
Sbjct: 56 WAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGTIIN- 114
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
++++ V D+ +DVN+ V+ S + + + +F + H
Sbjct: 115 -SPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYSDH 173
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M + YL ++ + K+ L Y+ ++C + ++FE + + + +
Sbjct: 174 GGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNIYA 233
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKDE-LDKKPKLTLLDQMKKYN 251
+++ + S G C S ++ L + W++D + TL Q +
Sbjct: 234 TTASNAEESSWGTYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMHNLQTETLHQQYELVK 293
Query: 252 YDYHK---ANSKLIINMENKQAQDIKAADFWK--PKQPML---NQVILRNDQDILAQQD 302
+ ++ + + P + L+ + Q+D
Sbjct: 294 RRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNFTFADANSLKPPSRVTNQRD 352
>dbj|BAB29018.1| (AK013853) data source:SPTR, source key:O14822,
evidence:ISS~homolog to GPI TRANSAMIDASE~putative [Mus
musculus]
Length = 395
Score = 435 bits (1108), Expect = e-121
Identities = 66/283 (23%), Positives = 136/283 (47%), Gaps = 4/283 (1%)
Query: 19 NQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACA 78
N +++ TS+FWFNYR N+L +Y+ +K I D I LM+ +D ACN +N P +
Sbjct: 45 NWAVLVCTSRFWFNYRHVANTLSVYRSVKRLGIPDSHIVLMLADDMACNARNPKPATVFS 104
Query: 79 QDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQSRRLTLSKEQKLLMFMNGH 138
+V D++ ++ ++ V+ + TP+S+RL + +L++M GH
Sbjct: 105 HKNMELNVYGDDVEVDYRSYRGTVENFLRVLTXEVPPSTPRSKRLLSDDKSNILIYMTGH 164
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG+G+ K QD+ + + E+ ++M + Y E I D+C +++E + N++ L
Sbjct: 165 GGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGASMYERFYSPNIMALA 224
Query: 199 SSSLGEKAYSHGRCSILSISKTDKFSLTTHYWLKDELDKKPKLTLLDQMKKYNYDYHKAN 258
SS +GE + SH + + D+++ + K E++ + + D + +
Sbjct: 225 SSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFWK-EINPASQTNMNDLFQVCPKSLCVST 283
Query: 259 SKLIINMENKQAQDIKAADFWKPKQP---MLNQVILRNDQDIL 298
++ + +++ DF+ + ++ L+ D ++
Sbjct: 284 PGHRTDLFQRDPKNVLITDFFGSVRKVEITTEKISLQWDSQVV 326
>ref|NP_563825.1| (NM_100749) putative GPI-anchor transamidase (U32517) [Arabidopsis
thaliana]
Length = 388
Score = 432 bits (1101), Expect = e-120
Identities = 70/272 (25%), Positives = 139/272 (50%), Gaps = 1/272 (0%)
Query: 19 NQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACA 78
N +++ TS+FWFNYR N+L +Y+ +K I D++I LM+ +D ACN +N P
Sbjct: 27 NWAVLVCTSRFWFNYRHMANTLSLYRTVKRLGIPDERIILMLADDMACNARNEYPAQVFN 86
Query: 79 QDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQSRRLTLSKEQKLLMFMNGH 138
+ + NV D++ +V ++ ++ V+ ++ P+S+RL + +L++M GH
Sbjct: 87 NENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVPRSKRLLSDEGSHILLYMTGH 146
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GGD + K QD L ++ K+M+ + ++E ++ D+C A TLF +++ ++ +G
Sbjct: 147 GGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTCQAATLFNQLQSPGVLAIG 206
Query: 199 SSSLGEKAYSHGRCSILSISKTDKFSLTTHYWLKDELDKKPKLTLLDQMKKYNYDYHKAN 258
SS GE +YSH S + +S D+F+ T + + L+ +L + Y+ +
Sbjct: 207 SSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFE-RLNIYDNASLNSLFRSYDPRLLMST 265
Query: 259 SKLIINMENKQAQDIKAADFWKPKQPMLNQVI 290
+ ++ ++ +F+ ++
Sbjct: 266 AYYRTDLYQPHLVEVPVTNFFGSVMETIHTDS 297
>emb|CAC43295.1| (AJ309173) putative vacuolar processing enzyme [Beta vulgaris]
Length = 486
Score = 432 bits (1101), Expect = e-120
Identities = 51/299 (17%), Positives = 111/299 (37%), Gaps = 18/299 (6%)
Query: 20 QYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQ 79
++++ S ++NYR + YQ LK+ + D+ I + + +D A + +N PGV
Sbjct: 52 WAVLIAGSSGYWNYRHQADVCHAYQVLKKGGLKDENIIVFMYDDIAYDEENPRPGVLIN- 110
Query: 80 DGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGH 138
+++ V D+ DV + + + + T S + + + +F + H
Sbjct: 111 -SPYGHDVYAGVPKDYTGEDVTVNNFFAAILGNKDAITGGSGKVVNSGPNDHIFIFYSDH 169
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GG G M YL E+ + KE Y+ + ++C + ++FE + + + +
Sbjct: 170 GGAGVLGMPTYPYLYADELIETLKEKHASGTYKSLVVYIEACESGSIFEGILPEGLNIYA 229
Query: 199 SSSLGEKAYSHG-RCSILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYN 251
+++ S G C + ++ L + W++D E +L Q +
Sbjct: 230 TTASNAVESSWGTYCPGQDPNVPPEYDTCLGDLYSVSWIEDSERHNLHTESLKQQYEVVK 289
Query: 252 YDYH--KANSKLIINMENKQAQDIKAADFWKPKQPMLN-----QVIL-RNDQDILAQQD 302
++ +K+ + N L + + Q+D
Sbjct: 290 TKTAEKPFYGSHVMQYGDKELTQDMLYLYMGTNPNNENYTYVDDNSLHPTSSNAVNQRD 348
>gb|AAL29895.1| (AY057228) GPI8 transamidase [Paramecium tetraurelia]
Length = 309
Score = 426 bits (1086), Expect = e-118
Identities = 309/309 (100%), Positives = 309/309 (100%)
Query: 1 MIIFILFTAILCYRPIPQNQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMI 60
MIIFILFTAILCYRPIPQNQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMI
Sbjct: 1 MIIFILFTAILCYRPIPQNQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMI 60
Query: 61 PEDTACNRKNNVPGVACAQDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS 120
PEDTACNRKNNVPGVACAQDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS
Sbjct: 61 PEDTACNRKNNVPGVACAQDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQS 120
Query: 121 RRLTLSKEQKLLMFMNGHGGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSC 180
RRLTLSKEQKLLMFMNGHGGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSC
Sbjct: 121 RRLTLSKEQKLLMFMNGHGGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSC 180
Query: 181 GAITLFETVKAQNMILLGSSSLGEKAYSHGRCSILSISKTDKFSLTTHYWLKDELDKKPK 240
GAITLFETVKAQNMILLGSSSLGEKAYSHGRCSILSISKTDKFSLTTHYWLKDELDKKPK
Sbjct: 181 GAITLFETVKAQNMILLGSSSLGEKAYSHGRCSILSISKTDKFSLTTHYWLKDELDKKPK 240
Query: 241 LTLLDQMKKYNYDYHKANSKLIINMENKQAQDIKAADFWKPKQPMLNQVILRNDQDILAQ 300
LTLLDQMKKYNYDYHKANSKLIINMENKQAQDIKAADFWKPKQPMLNQVILRNDQDILAQ
Sbjct: 241 LTLLDQMKKYNYDYHKANSKLIINMENKQAQDIKAADFWKPKQPMLNQVILRNDQDILAQ 300
Query: 301 QDFLKLMNA 309
QDFLKLMNA
Sbjct: 301 QDFLKLMNA 309
>gb|AAF45703.1| (AE003422) EG:133E12.3 gene product [Drosophila melanogaster]
Length = 322
Score = 421 bits (1072), Expect = e-117
Identities = 68/267 (25%), Positives = 131/267 (48%), Gaps = 1/267 (0%)
Query: 19 NQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACA 78
N +++ S+FWFNYR N L IY+ +K I D QI LMI +D ACN +N PG
Sbjct: 42 NWAVLVDASRFWFNYRHVANVLSIYRSVKRLGIPDSQIILMIADDMACNARNPRPGQVYN 101
Query: 79 QDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQSRRLTLSKEQKLLMFMNGH 138
Q +V D++ +V ++ ++ ++ + T +S++L +L+++ GH
Sbjct: 102 NANQHINVYGDDVEVDYRGYEVTVENFVRLLTGRTQNGTARSKKLLSDAGSNVLIYLTGH 161
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GGDG+ K QD+ + E+ ++M + Y E F + D+C A +L+E + N++ +
Sbjct: 162 GGDGFLKFQDSEEITSQELADGIQQMWEKKRYNELFFMVDTCQAASLYEKFTSPNVLAVA 221
Query: 199 SSSLGEKAYSHGRCSILSISKTDKFSLTTHYWLKDELDKKPKLTLLDQMKKYNYDYHKAN 258
SS +GE + SH + + D+++ +L+ + K T+ + ++ +
Sbjct: 222 SSLVGEDSLSHHVDPSIGVYMIDRYTYYALEFLEK-VQPFSKRTIGEFLQVCPKRVCIST 280
Query: 259 SKLIINMENKQAQDIKAADFWKPKQPM 285
+ ++ + + DF+ +P
Sbjct: 281 VGVRKDLYPRDPHKVPITDFFGAIRPT 307
>pir||T13411 hypothetical protein 133E12.3 - fruit fly (Drosophila melanogaster)
emb|CAA15687.1| (AL009192) /prediction=(method:''genefinder'', version:''084'',
score:''46.36'')~/prediction=(method:''genscan'',
version:''1.0'',
score:''114.92'')~/match=(desc:''GPI-ANCHOR TRANSMIDASE
(EC 3.-.-.-)'', species:''SACCHAROMYCES CEREVISIAE
(BAKER'S YEAST>
Length = 326
Score = 420 bits (1070), Expect = e-116
Identities = 68/267 (25%), Positives = 131/267 (48%), Gaps = 1/267 (0%)
Query: 19 NQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACA 78
N +++ S+FWFNYR N L IY+ +K I D QI LMI +D ACN +N PG
Sbjct: 46 NWAVLVDASRFWFNYRHVANVLSIYRSVKRLGIPDSQIILMIADDMACNARNPRPGQVYN 105
Query: 79 QDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQSRRLTLSKEQKLLMFMNGH 138
Q +V D++ +V ++ ++ ++ + T +S++L +L+++ GH
Sbjct: 106 NANQHINVYGDDVEVDYRGYEVTVENFVRLLTGRTQNGTARSKKLLSDAGSNVLIYLTGH 165
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GGDG+ K QD+ + E+ ++M + Y E F + D+C A +L+E + N++ +
Sbjct: 166 GGDGFLKFQDSEEITSQELADGIQQMWEKKRYNELFFMVDTCQAASLYEKFTSPNVLAVA 225
Query: 199 SSSLGEKAYSHGRCSILSISKTDKFSLTTHYWLKDELDKKPKLTLLDQMKKYNYDYHKAN 258
SS +GE + SH + + D+++ +L+ + K T+ + ++ +
Sbjct: 226 SSLVGEDSLSHHVDPSIGVYMIDRYTYYALEFLEK-VQPFSKRTIGEFLQVCPKRVCIST 284
Query: 259 SKLIINMENKQAQDIKAADFWKPKQPM 285
+ ++ + + DF+ +P
Sbjct: 285 VGVRKDLYPRDPHKVPITDFFGAIRPT 311
>emb|CAB42655.1| (AJ238882) putative preprolegumain [Vicia narbonensis]
Length = 380
Score = 414 bits (1054), Expect = e-114
Identities = 43/282 (15%), Positives = 105/282 (36%), Gaps = 20/282 (7%)
Query: 38 NSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQDGQREPNLHKNVNWDFKR 97
+ YQ L++ + ++ I + + +D A + +N PGV N+++ V D+
Sbjct: 1 DVCHAYQLLRKGGLKEENIIVFMYDDIAYSEENPRPGVIIN--SPHGENVYEGVPKDYTG 58
Query: 98 NDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGHGGDGYTKMQDTTYLLDFE 156
DV + + + + + S + + ++ +F + HGG G M + Y+ +
Sbjct: 59 EDVTVGNFFAALLGNKSALSGGSGKVVDSGPNDRIFVFYSDHGGPGVLGMPTSPYMYASD 118
Query: 157 MEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLGSSSLGEKAYSHG-RCSIL 215
+ ++ K Y+ ++C + ++FE + + + + +++ + S G C
Sbjct: 119 LVEVLKIKHAAGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTAANAEESSWGTYCPGE 178
Query: 216 SISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYNYDYHKAN---SKLIINME 266
+ S ++ L + W++D ++ TL Q + N ++
Sbjct: 179 NPSPPPEYETCLADLYSVAWMEDSDIHNLQTETLHQQYELVKERTSNGNSNYGSHVMQYG 238
Query: 267 NKQAQDIKAADFWKPKQPMLNQ------VILRNDQDILAQQD 302
+ + + P L + Q+D
Sbjct: 239 DIELSKDSLFLYLGS-NPSNENFTFVGRNSLVPPSKAINQRD 279
>emb|CAB42651.1| (AJ238881) putative preprolegumain [Nicotiana tabacum]
Length = 419
Score = 409 bits (1043), Expect = e-113
Identities = 48/283 (16%), Positives = 110/283 (37%), Gaps = 17/283 (6%)
Query: 35 QAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACAQDGQREPNLHKNVNWD 94
+ YQ LK + D+ I + + +D A + N PGV +++ V D
Sbjct: 1 HQADVCHAYQILKRGGLKDENIVVFMYDDIAKSELNPRPGVIINHPN--GSDVYAGVPKD 58
Query: 95 FKRNDVNIKYWIDVMRNKYNRYTPQS-RRLTLSKEQKLLMFMNGHGGDGYTKMQDTTYLL 153
+ V V+ + S + + ++ ++ + HGG G M + +L
Sbjct: 59 YTGEHVTAANLYAVLLGDKSAVKGGSGKIVDSKPNDRIFLYYSDHGGPGVLGMPNMPFLY 118
Query: 154 DFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLGSSSLGEKAYSHG-RC 212
+ ++ K+ Y+E L ++C + ++FE + +++ + +++ + S G C
Sbjct: 119 AKDFIEVLKKKHAAGTYKEMVLYIEACESGSVFEGMMPEDLNIYVTTASNAEESSWGTYC 178
Query: 213 SILSISKTDKF-----SLTTHYWLKD-ELDKKPKLTLLDQMKKYNYDYHKAN----SKLI 262
+ ++ L + W++D E K T+ Q +K N +
Sbjct: 179 PGMDPPPPPEYITCLGDLYSVAWMEDSESHNLKKETIKQQYEKVKERTSNFNNYNAGSHV 238
Query: 263 INMENKQAQDIKAADF--WKPKQPMLNQVIL-RNDQDILAQQD 302
+ +K+ + K + + P L+ + +++ Q+D
Sbjct: 239 MEYGSKEIKPEKVYLYQGFDPATANLSANKIAFAHVEVVNQRD 281
>ref|NP_010618.1| (NC_001136) Protein involved in the attachment of
glycosylphosphatidylinositol (GPI) anchors to proteins;
Gpi8p [Saccharomyces cerevisiae]
sp|P49018|GPI8_YEAST GPI-ANCHOR TRANSAMIDASE
pir||S59796 probable membrane protein YDR331w - yeast (Saccharomyces
cerevisiae)
gb|AAB64766.1| (U32517) Ydr331wp [Saccharomyces cerevisiae]
Length = 411
Score = 403 bits (1027), Expect = e-111
Identities = 72/272 (26%), Positives = 141/272 (51%), Gaps = 1/272 (0%)
Query: 19 NQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACA 78
N +++STS+FWFNYR N L +Y+ +K I D QI LM+ +D ACN +N PG
Sbjct: 38 NWAVLVSTSRFWFNYRHMANVLSMYRTVKRLGIPDSQIILMLSDDVACNSRNLFPGSVFN 97
Query: 79 QDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQSRRLTLSKEQKLLMFMNGH 138
+V D++ +V ++ +I ++ +++ P+S+RL + + ++M GH
Sbjct: 98 NKDHAIDLYGDSVEVDYRGYEVTVENFIRLLTDRWTEDHPKSKRLLTDENSNIFIYMTGH 157
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GGD + K QD + ++ ++M + Y E F + D+C A T++ + N++ +G
Sbjct: 158 GGDDFLKFQDAEEIASEDIADAFQQMYEKKRYNEIFFMIDTCQANTMYSKFYSPNILAVG 217
Query: 199 SSSLGEKAYSHGRCSILSISKTDKFSLTTHYWLKDELDKKPKLTLLDQMKKYNYDYHKAN 258
SS + E +YSH + ++ D+F+ +L+ ++DK LTL D + ++ ++
Sbjct: 218 SSEMDESSYSHHSDVEIGVAVIDRFTYYCLDFLE-QIDKNSTLTLQDLFDSFTFEKIHSH 276
Query: 259 SKLIINMENKQAQDIKAADFWKPKQPMLNQVI 290
+ ++ ++ ++ DF+ Q ++
Sbjct: 277 VGVRTDLFDRNPSEVLITDFFANVQNVIPDDS 308
>pir||T00731 hypothetical protein F22O13.26 - Arabidopsis thaliana
Length = 428
Score = 402 bits (1024), Expect = e-111
Identities = 63/276 (22%), Positives = 134/276 (47%), Gaps = 5/276 (1%)
Query: 19 NQYIILSTSKFWFNYRQAI----NSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPG 74
N +++ TS+F + + + L + + +K I D++I LM+ +D ACN +N P
Sbjct: 27 NWAVLVCTSRFCSLHSLVLTFIFSLLGVSRTVKRLGIPDERIILMLADDMACNARNEYPA 86
Query: 75 VACAQDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQSRRLTLSKEQKLLMF 134
+ + NV D++ +V ++ ++ V+ ++ P+S+RL + +L++
Sbjct: 87 QVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVPRSKRLLSDEGSHILLY 146
Query: 135 MNGHGGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNM 194
M GHGGD + K QD L ++ K+M+ + ++E ++ D+C A TLF +++ +
Sbjct: 147 MTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTCQAATLFNQLQSPGV 206
Query: 195 ILLGSSSLGEKAYSHGRCSILSISKTDKFSLTTHYWLKDELDKKPKLTLLDQMKKYNYDY 254
+ +GSS GE +YSH S + +S D+F+ T + + L+ +L + Y+
Sbjct: 207 LAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFE-RLNIYDNASLNSLFRSYDPRL 265
Query: 255 HKANSKLIINMENKQAQDIKAADFWKPKQPMLNQVI 290
+ + ++ ++ +F+ ++
Sbjct: 266 LMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHTDS 301
>ref|NP_071562.1| (NM_022226) protease, cysteine, 1 (legumain) [Rattus norvegicus]
dbj|BAA84750.1| (AB032766) legumain [Rattus norvegicus]
Length = 435
Score = 402 bits (1024), Expect = e-111
Identities = 55/281 (19%), Positives = 117/281 (41%), Gaps = 15/281 (5%)
Query: 18 QNQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVAC 77
++ +I++ S W+NYR ++ YQ + I D+QI +M+ +D A N +N PGV
Sbjct: 30 KHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIIVMMYDDIANNEENPTPGVVI 89
Query: 78 AQDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRY--TPQSRRLTLSKEQKLLMFM 135
+ +++K V D+ DV + ++ V+R + L + ++
Sbjct: 90 NRPN--GTDVYKGVPKDYTGEDVTPENFLAVLRGDEEAVKGKGSGKVLKSGPRDHVFVYF 147
Query: 136 NGHGGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMI 195
HG G + L ++ K + M ++YQ+ ++C + ++ + ++
Sbjct: 148 TDHGATGILVFPN-EDLHVKDLNKTIRYMYEHKMYQKMVFYIEACESGSMMNHL-PDDID 205
Query: 196 LLGSSSL--GEKAYSHGRCSILSISKTDKFSLTTHYWLKD-ELDKKPKLTLLDQMKKYNY 252
+ +++ E +Y+ S D + W++D +++ K TL K+Y+
Sbjct: 206 VYATTAANPNESSYACYYDEERSTYLGDW---YSVNWMEDSDVEDLTKETLH---KQYHL 259
Query: 253 DYHKANSKLIINMENKQAQDIKAADFWKPKQPMLNQVILRN 293
N+ ++ NK +K F K + + L
Sbjct: 260 VKSHTNTSHVMQYGNKSISTMKVMQFQGMKHRASSPISLPP 300
>gb|AAF73260.1|AF154349_1 (AF154349) legumain [Rattus norvegicus]
Length = 435
Score = 402 bits (1024), Expect = e-111
Identities = 55/281 (19%), Positives = 117/281 (41%), Gaps = 15/281 (5%)
Query: 18 QNQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVAC 77
++ +I++ S W+NYR ++ YQ + I D+QI +M+ +D A N +N PGV
Sbjct: 30 KHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIIVMMYDDIANNEENPTPGVVI 89
Query: 78 AQDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRY--TPQSRRLTLSKEQKLLMFM 135
+ +++K V D+ DV + ++ V+R + L + ++
Sbjct: 90 NRPN--GTDVYKGVPKDYTGEDVTPENFLAVLRGDEEAVKGKGSGKVLKSGPRDHVFVYF 147
Query: 136 NGHGGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMI 195
HG G + L ++ K + M ++YQ+ ++C + ++ + ++
Sbjct: 148 TDHGATGILVFPN-EDLHVKDLNKTIRYMYEHKMYQKMVFYIEACESGSMMNHL-PDDID 205
Query: 196 LLGSSSL--GEKAYSHGRCSILSISKTDKFSLTTHYWLKD-ELDKKPKLTLLDQMKKYNY 252
+ +++ E +Y+ S D + W++D +++ K TL K+Y+
Sbjct: 206 VYATTAANPNESSYACYYDEERSTYLGDW---YSVNWMEDSDVEDLTKETLH---KQYHL 259
Query: 253 DYHKANSKLIINMENKQAQDIKAADFWKPKQPMLNQVILRN 293
N+ ++ NK +K F K + + L
Sbjct: 260 VKSHTNTSHVMQYGNKSISTMKVMQFQGMKHRASSPISLPP 300
>ref|NP_035305.1| (NM_011175) protease, cysteine, 1; preprolegumain [Mus musculus]
emb|CAA04439.1| (AJ000990) legumain [Mus musculus]
gb|AAF21659.1|AF044266_1 (AF044266) preprolegumain; cysteine Protease [Mus musculus]
Length = 435
Score = 400 bits (1018), Expect = e-110
Identities = 53/281 (18%), Positives = 116/281 (40%), Gaps = 15/281 (5%)
Query: 18 QNQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVAC 77
++ +I++ S W+NYR ++ YQ + I D+QI +M+ +D A + +N PGV
Sbjct: 30 KHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIIVMMYDDIANSEENPTPGVVI 89
Query: 78 AQDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRY--TPQSRRLTLSKEQKLLMFM 135
+ +++K V D+ DV + ++ V+R + L + ++
Sbjct: 90 NRPN--GTDVYKGVLKDYTGEDVTPENFLAVLRGDAEAVKGKGSGKVLKSGPRDHVFIYF 147
Query: 136 NGHGGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMI 195
HG G + L ++ K + M ++YQ+ ++C + ++ + ++
Sbjct: 148 TDHGATGILVFPN-DDLHVKDLNKTIRYMYEHKMYQKMVFYIEACESGSMMNHL-PDDIN 205
Query: 196 LLGSSSLG--EKAYSHGRCSILSISKTDKFSLTTHYWLKD-ELDKKPKLTLLDQMKKYNY 252
+ +++ E +Y+ D + W++D +++ K TL K+Y+
Sbjct: 206 VYATTAANPKESSYACYYDEERGTYLGDW---YSVNWMEDSDVEDLTKETLH---KQYHL 259
Query: 253 DYHKANSKLIINMENKQAQDIKAADFWKPKQPMLNQVILRN 293
N+ ++ NK +K F K + + L
Sbjct: 260 VKSHTNTSHVMQYGNKSISTMKVMQFQGMKHRASSPISLPP 300
>dbj|BAB69947.1| (AB060129) legumain [Bos taurus]
Length = 433
Score = 396 bits (1009), Expect = e-109
Identities = 51/284 (17%), Positives = 114/284 (39%), Gaps = 15/284 (5%)
Query: 18 QNQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVAC 77
++ +I++ S W+NYR ++ YQ + I D+QI +M+ +D A + N PG+
Sbjct: 28 KHWVVIVAGSNGWYNYRHQADACHAYQIVHRNGIPDEQIIVMMYDDIANSEDNPTPGIVI 87
Query: 78 AQDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYT--PQSRRLTLSKEQKLLMFM 135
+ ++++ V D+ DV K ++ V+R + L + ++
Sbjct: 88 NRPN--GSDVYQGVLKDYTGEDVTPKNFLAVLRGDAEAVKGVGSGKVLKSGPRDHVFVYF 145
Query: 136 NGHGGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMI 195
HG G + L ++ + + M ++YQ+ ++C + ++ + ++
Sbjct: 146 TDHGATGILVFPN-EDLHVKDLNETIRYMYEHKMYQKMVFYIEACESGSMMNHL-PPDIN 203
Query: 196 LLGSSSLG--EKAYSHGRCSILSISKTDKFSLTTHYWLKD-ELDKKPKLTLLDQMKKYNY 252
+ +++ E +Y+ S D + W++D +++ K TL Q +
Sbjct: 204 VYATTAANPRESSYACYYDEQRSTFLGDW---YSVNWMEDSDVEDLTKETLHKQYQLVK- 259
Query: 253 DYHKANSKLIINMENKQAQDIKAADFWKPKQPMLNQVILRNDQD 296
N+ ++ NK +K F K + + L
Sbjct: 260 --SHTNTSHVMQYGNKSISAMKLMQFQGLKHQASSPISLPAVSR 301
>dbj|BAA09530.1| (D55696) cysteine protease [Homo sapiens]
Length = 433
Score = 396 bits (1008), Expect = e-109
Identities = 52/281 (18%), Positives = 117/281 (41%), Gaps = 15/281 (5%)
Query: 18 QNQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVAC 77
++ +I++ S W+NYR ++ YQ + I D+QI +M+ +D A + N PG+
Sbjct: 28 KHWAVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVI 87
Query: 78 AQDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYT--PQSRRLTLSKEQKLLMFM 135
+ ++++ V D+ DV + ++ V+R + L + + ++
Sbjct: 88 NRPN--GTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYF 145
Query: 136 NGHGGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMI 195
HG G + L ++ + M ++Y++ ++C + ++ + N+
Sbjct: 146 TDHGSTGILVFPN-EDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHL-PDNIN 203
Query: 196 LLGSSSLG--EKAYSHGRCSILSISKTDKFSLTTHYWLKD-ELDKKPKLTLLDQMKKYNY 252
+ +++ E +Y+ S D + W++D +++ K TL K+Y+
Sbjct: 204 VYATTAANPRESSYACYYDEKRSTYLGDW---YSVNWMEDSDVEDLTKETLH---KQYHL 257
Query: 253 DYHKANSKLIINMENKQAQDIKAADFWKPKQPMLNQVILRN 293
N+ ++ NK +K F K+ + V L
Sbjct: 258 VKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPP 298
>ref|XP_007399.4| (XM_007399) legumain [Homo sapiens]
Length = 401
Score = 395 bits (1007), Expect = e-109
Identities = 52/281 (18%), Positives = 117/281 (41%), Gaps = 15/281 (5%)
Query: 18 QNQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVAC 77
++ +I++ S W+NYR ++ YQ + I D+QI +M+ +D A + N PG+
Sbjct: 28 KHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVI 87
Query: 78 AQDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYT--PQSRRLTLSKEQKLLMFM 135
+ ++++ V D+ DV + ++ V+R + L + + ++
Sbjct: 88 NRPN--GTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYF 145
Query: 136 NGHGGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMI 195
HG G + L ++ + M ++Y++ ++C + ++ + N+
Sbjct: 146 TDHGSTGILVFPN-EDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHL-PDNIN 203
Query: 196 LLGSSSLG--EKAYSHGRCSILSISKTDKFSLTTHYWLKD-ELDKKPKLTLLDQMKKYNY 252
+ +++ E +Y+ S D + W++D +++ K TL K+Y+
Sbjct: 204 VYATTAANPRESSYACYYDEKRSTYLGDW---YSVNWMEDSDVEDLTKETLH---KQYHL 257
Query: 253 DYHKANSKLIINMENKQAQDIKAADFWKPKQPMLNQVILRN 293
N+ ++ NK +K F K+ + V L
Sbjct: 258 VKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPP 298
>gb|AAH03061.1|AAH03061 (BC003061) protease, cysteine, 1 (legumain) [Homo sapiens]
Length = 433
Score = 394 bits (1003), Expect = e-108
Identities = 52/281 (18%), Positives = 117/281 (41%), Gaps = 15/281 (5%)
Query: 18 QNQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVAC 77
++ +I++ S W+NYR ++ YQ + I D+QI +M+ +D A + N PG+
Sbjct: 28 KHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVI 87
Query: 78 AQDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYT--PQSRRLTLSKEQKLLMFM 135
+ ++++ V D+ DV + ++ V+R + L + + ++
Sbjct: 88 NRPN--GTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYF 145
Query: 136 NGHGGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMI 195
HG G + L ++ + M ++Y++ ++C + ++ + N+
Sbjct: 146 TDHGSTGILVFPN-EDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHL-PDNIN 203
Query: 196 LLGSSSLG--EKAYSHGRCSILSISKTDKFSLTTHYWLKD-ELDKKPKLTLLDQMKKYNY 252
+ +++ E +Y+ S D + W++D +++ K TL K+Y+
Sbjct: 204 VYATTAANPRESSYACYYDEKRSTYLGDW---YSVNWMEDSDVEDLTKETLH---KQYHL 257
Query: 253 DYHKANSKLIINMENKQAQDIKAADFWKPKQPMLNQVILRN 293
N+ ++ NK +K F K+ + V L
Sbjct: 258 VKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPP 298
>ref|NP_005597.1| (NM_005606) legumain; protease, cysteine, 1 (legumain) [Homo
sapiens]
sp|Q99538|LEGU_HUMAN Legumain precursor (Asparaginyl endopeptidase)
emb|CAA70989.1| (Y09862) legumain [Homo sapiens]
gb|AAH08004.1|AAH08004 (BC008004) protease, cysteine, 1 (legumain) [Homo sapiens]
Length = 433
Score = 394 bits (1003), Expect = e-108
Identities = 52/281 (18%), Positives = 117/281 (41%), Gaps = 15/281 (5%)
Query: 18 QNQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVAC 77
++ +I++ S W+NYR ++ YQ + I D+QI +M+ +D A + N PG+
Sbjct: 28 KHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVI 87
Query: 78 AQDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYT--PQSRRLTLSKEQKLLMFM 135
+ ++++ V D+ DV + ++ V+R + L + + ++
Sbjct: 88 NRPN--GTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYF 145
Query: 136 NGHGGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMI 195
HG G + L ++ + M ++Y++ ++C + ++ + N+
Sbjct: 146 TDHGSTGILVFPN-EDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHL-PDNIN 203
Query: 196 LLGSSSLG--EKAYSHGRCSILSISKTDKFSLTTHYWLKD-ELDKKPKLTLLDQMKKYNY 252
+ +++ E +Y+ S D + W++D +++ K TL K+Y+
Sbjct: 204 VYATTAANPRESSYACYYDEKRSTYLGDW---YSVNWMEDSDVEDLTKETLH---KQYHL 257
Query: 253 DYHKANSKLIINMENKQAQDIKAADFWKPKQPMLNQVILRN 293
N+ ++ NK +K F K+ + V L
Sbjct: 258 VKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPP 298
>gb|AAF99765.1|AC003981_15 (AC003981) F22O13.24 [Arabidopsis thaliana]
Length = 407
Score = 390 bits (994), Expect = e-107
Identities = 63/272 (23%), Positives = 129/272 (47%), Gaps = 18/272 (6%)
Query: 19 NQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACA 78
N +++ TS+FW +K I D++I LM+ +D ACN +N P
Sbjct: 27 NWAVLVCTSRFW--------------TVKRLGIPDERIILMLADDMACNARNEYPAQVFN 72
Query: 79 QDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQSRRLTLSKEQKLLMFMNGH 138
+ + NV D++ +V ++ ++ V+ ++ P+S+RL + +L++M GH
Sbjct: 73 NENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVPRSKRLLSDEGSHILLYMTGH 132
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GGD + K QD L ++ K+M+ + ++E ++ D+C A TLF +++ ++ +G
Sbjct: 133 GGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTCQAATLFNQLQSPGVLAIG 192
Query: 199 SSSLGEKAYSHGRCSILSISKTDKFSLTTHYWLKDELDKKPKLTLLDQMKKYNYDYHKAN 258
SS GE +YSH S + +S D+F+ T + + L+ +L + Y+ +
Sbjct: 193 SSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFE-RLNIYDNASLN---RSYDPRLLMST 248
Query: 259 SKLIINMENKQAQDIKAADFWKPKQPMLNQVI 290
+ ++ ++ +F+ ++
Sbjct: 249 AYYRTDLYQPHLVEVPVTNFFGSVMETIHTDS 280
>pir||T40853 probable cysteine proteinase (EC 3.4.22.-) [similarity] - fission
yeast (Schizosaccharomyces pombe)
emb|CAB57844.1| (AL121783) putative gpi-anchor transamidase [Schizosaccharomyces
pombe]
emb|CAC13970.1| (AJ250428) GPI8p transamidase [Schizosaccharomyces pombe]
Length = 380
Score = 389 bits (990), Expect = e-107
Identities = 83/267 (31%), Positives = 143/267 (53%), Gaps = 2/267 (0%)
Query: 19 NQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACA 78
N +++STS+FWFNYR N L IY+ +K I D QI LMI +D ACN +N PG
Sbjct: 26 NWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRNLFPGTVFD 85
Query: 79 QDGQREPNLHKNVNWDFKRNDVNIKYWIDVMRNKYNRYTPQSRRLTLSKEQKLLMFMNGH 138
+ + + D+K +V ++ +I ++ + TP S+RL ++ +L++M GH
Sbjct: 86 NADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPENTPASKRLLTNERSNILIYMTGH 145
Query: 139 GGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILLG 198
GGDG+ K QD L ++ +++ + Y E + D+C A +L+ + + N++ +G
Sbjct: 146 GGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKIYSPNVLAIG 205
Query: 199 SSSLGEKAYSHGRCSILSISKTDKFSLTTHYWLKDELDKKPKLTLLDQMKKYN-YDYHKA 257
SS +G +YSH + ++ D+F+ + +L++ +D K KLT+ D + YN Y+ H
Sbjct: 206 SSEVGTSSYSHHADIDIGVAVIDRFTFSNLEFLENRVDSKSKLTMQDLINSYNPYEIHST 265
Query: 258 NSKLIINMENKQAQDIKAADFWKPKQP 284
IN+ + DI DF+ +
Sbjct: 266 PGVQPINLR-RSPDDILITDFFGNVRD 291
>ref|NP_502076.1| (NM_069675) haemoglobinase like [Caenorhabditis elegans]
sp|P49048|YJ96_CAEEL Hypothetical 36.9 kDa protein T05E11.6 in chromosome IV
pir||T24525 hypothetical protein T05E11.6 - Caenorhabditis elegans
emb|CAA92977.1| (Z68751) Similarity to Schistosoma Heamoglobinase (SW:HGLB_SCHMA),
contains similarity to Pfam domain: PF01650 (Peptidase
C13 family), Score=620.4, E-value=3.3e-183, N=1
[Caenorhabditis elegans]
Length = 322
Score = 383 bits (975), Expect = e-105
Identities = 72/267 (26%), Positives = 138/267 (50%), Gaps = 4/267 (1%)
Query: 19 NQYIILSTSKFWFNYRQAINSLMIYQQLKEWRINDDQISLMIPEDTACNRKNNVPGVACA 78
N +++ TSKFWFNYR N L +Y +K I D I +M+ ED CN +N PG A
Sbjct: 41 NWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYA 100
Query: 79 QDGQREPNLHK-NVNWDFKRNDVNIKYWIDVMRNKYNRYTPQSRRLTLSKEQKLLMFMNG 137
+ NL+ +V D++ +V ++ +I V+ +++ TP+S+RL + +L+++ G
Sbjct: 101 --ARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATPRSKRLLTDHQSNVLIYLTG 158
Query: 138 HGGDGYTKMQDTTYLLDFEMEKITKEMEFLQLYQEAFLISDSCGAITLFETVKAQNMILL 197
HGGD + K QD+ L + ++ + M Y E +I+DSC + +++E + + N++ L
Sbjct: 159 HGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASMYEWIDSPNVLSL 218
Query: 198 GSSSLGEKAYSHGRCSILSISKTDKFSLTTHYWLKDELDKK-PKLTLLDQMKKYNYDYHK 256
SS E++YS+ + + + D+++ T +L E+ + D +
Sbjct: 219 SSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLTKEVKALNSSANMQDYIDSCPARKCL 278
Query: 257 ANSKLIINMENKQAQDIKAADFWKPKQ 283
+N+ + + K + ++ DF+ +
Sbjct: 279 SNTGVRKDHYPKDVKRVRVTDFFGSSR 305
CPU time: 98.93 user secs. 2.69 sys. secs 101.62 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.316 0.188 0.590
Gapped
Lambda K H
0.270 0.0659 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 254599427
Number of Sequences: 887402
Number of extensions: 16096105
Number of successful extensions: 29403
Number of sequences better than 10.0: 89
Number of HSP's better than 10.0 without gapping: 73
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 28954
Number of HSP's gapped (non-prelim): 96
length of query: 309
length of database: 277,845,442
effective HSP length: 56
effective length of query: 253
effective length of database: 228,150,930
effective search space: 57722185290
effective search space used: 57722185290
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.1 bits)
S2: 74 (32.7 bits)
