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GPI Anchor Biosynthesis Report: orf6.3368.prot (PIG-H family, Candida albicans)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (205 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!

 Results of PSI-Blast iteration 2

Distribution of 7 Blast Hits on the Query Sequence

Sequences with E-value BETTER than threshold (0.002)


Sequences producing significant alignments:


 ref|NP_004560.1|  (NM_004569) phosphatidylinositol glycan, class ...   161  1e-38

 pir||T40636  glycosylphosphatidylinositol anchor protein homolog ...   137  1e-31

 dbj|BAB32246.1|  (AK020902) data source:SPTR, source key:Q14442, ...   134  9e-31

 emb|CAD21200.1|  (AL669999) conserved hypothetical protein [Neuro...   133  3e-30

 gb|AAL62432.1|  (AY072440) putative protein [Arabidopsis thaliana]     116  3e-25

 ref|NP_195278.1|  (NM_119718) putative protein [Arabidopsis thali...   115  5e-25


Sequences with E-value WORSE than threshold 


 ref|XP_018042.2|  (XM_018042) similar to phosphatidylinositol gly...    34  1.5


Alignments


>ref|NP_004560.1| (NM_004569) phosphatidylinositol glycan, class H [Homo sapiens]
 pir||A48024 glycosylphosphatidylinositol anchor class H biosynthesis protein -
           human
 gb|AAA03545.1| (L19783) GPI-H [Homo sapiens]
 gb|AAH04100.1|AAH04100 (BC004100) phosphatidylinositol glycan, class H [Homo sapiens]
          Length = 188

 Score =  161 bits (404), Expect = 1e-38
 Identities = 34/112 (30%), Positives = 65/112 (57%), Gaps = 6/112 (5%)

Query: 78  LLILVVGIV-LLLCLQTPEDTLIVMRGIGIQLQSKKMWRFQSSDSFIPINNMIDLVIHEG 136
           + I ++G++  L  ++  ++TL+++  +GIQ+ S      + S +FI +  + D+VI+E 
Sbjct: 69  IFITLLGLLGYLHFVKIDQETLLIIDSLGIQMTSSYA-SGKESTTFIEMGKVKDIVINEA 127

Query: 137 FHDYGQVIFYLCVLTKANNDKNPITIVFPEFL---PRKDIFLTVWRLSRELL 185
            +   +VI+YLC+L K   + + I+ V P F    PR D  + V+R  +E+L
Sbjct: 128 IY-MQKVIYYLCILLKDPVEPHGISQVVPVFQSAKPRLDCLIEVYRSCQEIL 178





>pir||T40636 glycosylphosphatidylinositol anchor protein homolog - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAB39362.1| (AL049474) similar to glycosylphosphatidylinositol anchor
           [Schizosaccharomyces pombe]
          Length = 160

 Score =  137 bits (343), Expect = 1e-31
 Identities = 30/107 (28%), Positives = 59/107 (55%), Gaps = 9/107 (8%)

Query: 78  LLILVVGIVLLLCL-----QTPEDTLIVMRGIGIQLQSKKMWRFQSSDSFIPINNMIDLV 132
           ++I +V +  LL L         ++L V+R +G+Q     +  ++SS   IP++++ D+ 
Sbjct: 48  IIITLVELSFLLSLFHIISGVNHESLFVIRDLGVQTNCHSIVPWKSSSKLIPLDSIRDIF 107

Query: 133 IHEGFHDYGQVIFYLCVLTKANNDKNPITIVFPEFLPRKDIFLTVWR 179
           I+EGF  +  V +Y+ +  ++  +   I +VFP  LPR D+   V++
Sbjct: 108 INEGFRKF-DVCYYMGIAIESETE---IHVVFPTLLPRHDVLQKVYK 150





>dbj|BAB32246.1| (AK020902) data source:SPTR, source key:Q14442,
           evidence:ISS~homolog to PHOSPHATIDYLINOSITOL GLYCAN,
           CLASS H~putative [Mus musculus]
          Length = 167

 Score =  134 bits (336), Expect = 9e-31
 Identities = 26/92 (28%), Positives = 54/92 (58%), Gaps = 3/92 (3%)

Query: 78  LLILVVGIV-LLLCLQTPEDTLIVMRGIGIQLQSKKMWRFQSSDSFIPINNMIDLVIHEG 136
           + I ++G++  L  ++  ++TL+++  +GIQ+ S      + S +FI ++ + D++I+E 
Sbjct: 69  IFITILGLLGYLHFVKIDQETLLIIDSLGIQMTSSYA-SGKESTTFIEMDKVKDIIINEA 127

Query: 137 FHDYGQVIFYLCVLTKANNDKNPITIVFPEFL 168
            +   +VI+YLC+L K     + I+ V P F 
Sbjct: 128 IY-MQKVIYYLCILLKEPGKPHEISQVVPVFQ 158





>emb|CAD21200.1| (AL669999) conserved hypothetical protein [Neurospora crassa]
          Length = 263

 Score =  133 bits (332), Expect = 3e-30
 Identities = 35/111 (31%), Positives = 58/111 (51%), Gaps = 14/111 (12%)

Query: 85  IVLLLCLQTPEDTLIVMRGIGIQLQSK----KMWR-----FQSSDSFIPINNMIDLVIHE 135
           I L   + T  ++L+V+RG+GIQ+ S       +R     F     FIP   + D++I+E
Sbjct: 124 IALFARIHTT-ESLLVLRGLGIQMSSSVGGGNFFRLGGGTFMKRTRFIPTEKIQDILINE 182

Query: 136 GFHDYGQVIFYLCVLTKANNDKNPITIVFPEFLPRKDIFLTVWRLSRELLF 186
            F  + +V +YL ++ +   D   + + FP  LPR+ I   VWR +R  L+
Sbjct: 183 AFKGF-EVRYYLVIVVEGEQD---VVVCFPRLLPRRKIVERVWRGARGCLY 229





>gb|AAL62432.1| (AY072440) putative protein [Arabidopsis thaliana]
          Length = 195

 Score =  116 bits (289), Expect = 3e-25
 Identities = 20/97 (20%), Positives = 49/97 (49%), Gaps = 8/97 (8%)

Query: 85  IVLLLCLQ-TPEDTLIVMRGIGIQLQSKKMWRFQSSDSFIPINNMIDLVIHEGFHDYGQV 143
           +V+L   +   ++++I++   GIQL+++ +   ++   FIPI+ ++  V+ E        
Sbjct: 81  LVMLHSRKFVKKESVIILPTFGIQLETQYL-SGKTVSRFIPIDKILKPVLVECVTPI--T 137

Query: 144 IFY-LCVLTKANNDKNPITIVFPEFLPRKDIFLTVWR 179
            ++ L +  +       +T+VF E  P   + + +W+
Sbjct: 138 CYWSLSLFLRGEEQ---LTLVFKELRPPLKMLVPIWK 171





>ref|NP_195278.1| (NM_119718) putative protein [Arabidopsis thaliana]
 pir||T04658 hypothetical protein F8D20.40 - Arabidopsis thaliana
 emb|CAA20023.1| (AL031135) putative protein [Arabidopsis thaliana]
 emb|CAB80269.1| (AL161587) putative protein [Arabidopsis thaliana]
          Length = 204

 Score =  115 bits (287), Expect = 5e-25
 Identities = 20/97 (20%), Positives = 49/97 (49%), Gaps = 8/97 (8%)

Query: 85  IVLLLCLQ-TPEDTLIVMRGIGIQLQSKKMWRFQSSDSFIPINNMIDLVIHEGFHDYGQV 143
           +V+L   +   ++++I++   GIQL+++ +   ++   FIPI+ ++  V+ E        
Sbjct: 90  LVMLHSRKFVKKESVIILPTFGIQLETQYL-SGKTVSRFIPIDKILKPVLVECVTPI--T 146

Query: 144 IFY-LCVLTKANNDKNPITIVFPEFLPRKDIFLTVWR 179
            ++ L +  +       +T+VF E  P   + + +W+
Sbjct: 147 CYWSLSLFLRGEEQ---LTLVFKELRPPLKMLVPIWK 180





>ref|XP_018042.2| (XM_018042) similar to phosphatidylinositol glycan, class H (H.
          sapiens) [Homo sapiens]
          Length = 92

 Score = 34.3 bits (78), Expect = 1.5
 Identities = 4/22 (18%), Positives = 14/22 (63%), Gaps = 1/22 (4%)

Query: 78 LLILVVGIV-LLLCLQTPEDTL 98
          + I ++G++  L  ++  ++T+
Sbjct: 69 IFITLLGLLGYLHFVKIDQETV 90





CPU time:    46.85 user secs.	    1.67 sys. secs	   48.52 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.319    0.159    0.451 

Gapped
Lambda     K      H
   0.270   0.0559    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 119521480
Number of Sequences: 887402
Number of extensions: 5423986
Number of successful extensions: 16679
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 16655
Number of HSP's gapped (non-prelim): 18
length of query: 205
length of database: 277,845,442
effective HSP length: 54
effective length of query: 151
effective length of database: 229,925,734
effective search space: 34718785834
effective search space used: 34718785834
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.1 bits)
S2: 71 (31.8 bits)