Sequences with E-value BETTER than threshold (0.002) 244 5e-64
171 6e-42
155 4e-37
138 4e-32
138 6e-32
131 6e-30
48 9e-05
Sequences with E-value WORSE than threshold ref|NP_014360.1| (NC_001146) Glysosyl Phosphatidyl Inositol; Gpi... 39 0.065
gb|AAF54173.1| (AE003677) CG14463 gene product [Drosophila melan... 35 0.59
Alignments
>pir||T40636 glycosylphosphatidylinositol anchor protein homolog - fission yeast
(Schizosaccharomyces pombe)
emb|CAB39362.1| (AL049474) similar to glycosylphosphatidylinositol anchor
[Schizosaccharomyces pombe]
Length = 160
Score = 244 bits (618), Expect = 5e-64
Identities = 160/160 (100%), Positives = 160/160 (100%)
Query: 1 MLTIKRYPGAIEFTVHTSKSYGTQMFALCFISFVIGATSLAIGRSPKIIITLVELSFLLS 60
MLTIKRYPGAIEFTVHTSKSYGTQMFALCFISFVIGATSLAIGRSPKIIITLVELSFLLS
Sbjct: 1 MLTIKRYPGAIEFTVHTSKSYGTQMFALCFISFVIGATSLAIGRSPKIIITLVELSFLLS 60
Query: 61 LFHIISGVNHESLFVIRDLGVQTNCHSIVPWKSSSKLIPLDSIRDIFINEGFRKFDVCYY 120
LFHIISGVNHESLFVIRDLGVQTNCHSIVPWKSSSKLIPLDSIRDIFINEGFRKFDVCYY
Sbjct: 61 LFHIISGVNHESLFVIRDLGVQTNCHSIVPWKSSSKLIPLDSIRDIFINEGFRKFDVCYY 120
Query: 121 MGIAIESETEIHVVFPTLLPRHDVLQKVYKETVILLANNS 160
MGIAIESETEIHVVFPTLLPRHDVLQKVYKETVILLANNS
Sbjct: 121 MGIAIESETEIHVVFPTLLPRHDVLQKVYKETVILLANNS 160
>ref|NP_004560.1| (NM_004569) phosphatidylinositol glycan, class H [Homo sapiens]
pir||A48024 glycosylphosphatidylinositol anchor class H biosynthesis protein -
human
gb|AAA03545.1| (L19783) GPI-H [Homo sapiens]
gb|AAH04100.1|AAH04100 (BC004100) phosphatidylinositol glycan, class H [Homo sapiens]
Length = 188
Score = 171 bits (430), Expect = 6e-42
Identities = 45/145 (31%), Positives = 68/145 (46%), Gaps = 22/145 (15%)
Query: 20 SYGTQMFALCFISFVIGATSLAIGRSPKIIITLVELSFLLSLFHIISGVNHESLFVIRDL 79
+YG +F LC S ++ A I ITL+ L L H + ++ E+L +I L
Sbjct: 52 AYG--LFTLCENSMILSAA---------IFITLLGL---LGYLHFVK-IDQETLLIIDSL 96
Query: 80 GVQTNCHSIVPWKSSSKLIPLDSIRDIFINEGFRKFDVCYYMGIAIESETEIH------V 133
G+Q S K S+ I + ++DI INE V YY+ I ++ E H
Sbjct: 97 GIQMT-SSYASGKESTTFIEMGKVKDIVINEAIYMQKVIYYLCILLKDPVEPHGISQVVP 155
Query: 134 VFPTLLPRHDVLQKVYKETVILLAN 158
VF + PR D L +VY+ +LA+
Sbjct: 156 VFQSAKPRLDCLIEVYRSCQEILAH 180
>emb|CAD21200.1| (AL669999) conserved hypothetical protein [Neurospora crassa]
Length = 263
Score = 155 bits (389), Expect = 4e-37
Identities = 42/122 (34%), Positives = 67/122 (54%), Gaps = 11/122 (9%)
Query: 38 TSLAIGRSPKIIITLVELSFLLSLFHIISGVNHESLFVIRDLGVQTNCH---------SI 88
SLAI P ++ L L+ ++LF I ESL V+R LG+Q +
Sbjct: 103 ASLAISLPPYAVLALSALTSYIALFARIHTT--ESLLVLRGLGIQMSSSVGGGNFFRLGG 160
Query: 89 VPWKSSSKLIPLDSIRDIFINEGFRKFDVCYYMGIAIESETEIHVVFPTLLPRHDVLQKV 148
+ ++ IP + I+DI INE F+ F+V YY+ I +E E ++ V FP LLPR ++++V
Sbjct: 161 GTFMKRTRFIPTEKIQDILINEAFKGFEVRYYLVIVVEGEQDVVVCFPRLLPRRKIVERV 220
Query: 149 YK 150
++
Sbjct: 221 WR 222
>gb|AAL62432.1| (AY072440) putative protein [Arabidopsis thaliana]
Length = 195
Score = 138 bits (346), Expect = 4e-32
Identities = 23/96 (23%), Positives = 43/96 (43%), Gaps = 1/96 (1%)
Query: 55 LSFLLSLFHIISGVNHESLFVIRDLGVQTNCHSIVPWKSSSKLIPLDSIRDIFINEGFRK 114
LS L + H V ES+ ++ G+Q + K+ S+ IP+D I + E
Sbjct: 77 LSGFLVMLHSRKFVKKESVIILPTFGIQLETQ-YLSGKTVSRFIPIDKILKPVLVECVTP 135
Query: 115 FDVCYYMGIAIESETEIHVVFPTLLPRHDVLQKVYK 150
+ + + + E ++ +VF L P +L ++K
Sbjct: 136 ITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWK 171
>ref|NP_195278.1| (NM_119718) putative protein [Arabidopsis thaliana]
pir||T04658 hypothetical protein F8D20.40 - Arabidopsis thaliana
emb|CAA20023.1| (AL031135) putative protein [Arabidopsis thaliana]
emb|CAB80269.1| (AL161587) putative protein [Arabidopsis thaliana]
Length = 204
Score = 138 bits (345), Expect = 6e-32
Identities = 23/96 (23%), Positives = 43/96 (43%), Gaps = 1/96 (1%)
Query: 55 LSFLLSLFHIISGVNHESLFVIRDLGVQTNCHSIVPWKSSSKLIPLDSIRDIFINEGFRK 114
LS L + H V ES+ ++ G+Q + K+ S+ IP+D I + E
Sbjct: 86 LSGFLVMLHSRKFVKKESVIILPTFGIQLETQ-YLSGKTVSRFIPIDKILKPVLVECVTP 144
Query: 115 FDVCYYMGIAIESETEIHVVFPTLLPRHDVLQKVYK 150
+ + + + E ++ +VF L P +L ++K
Sbjct: 145 ITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWK 180
>dbj|BAB32246.1| (AK020902) data source:SPTR, source key:Q14442,
evidence:ISS~homolog to PHOSPHATIDYLINOSITOL GLYCAN,
CLASS H~putative [Mus musculus]
Length = 167
Score = 131 bits (328), Expect = 6e-30
Identities = 38/123 (30%), Positives = 57/123 (45%), Gaps = 22/123 (17%)
Query: 20 SYGTQMFALCFISFVIGATSLAIGRSPKIIITLVELSFLLSLFHIISGVNHESLFVIRDL 79
+YG +F LC S V+ AT I IT++ L L H + ++ E+L +I L
Sbjct: 52 AYG--LFTLCENSMVLSAT---------IFITILGL---LGYLHFVK-IDQETLLIIDSL 96
Query: 80 GVQTNCHSIVPWKSSSKLIPLDSIRDIFINEGFRKFDVCYYMGIAIESETEIH------V 133
G+Q S K S+ I +D ++DI INE V YY+ I ++ + H
Sbjct: 97 GIQMT-SSYASGKESTTFIEMDKVKDIIINEAIYMQKVIYYLCILLKEPGKPHEISQVVP 155
Query: 134 VFP 136
VF
Sbjct: 156 VFQ 158
>ref|XP_018042.2| (XM_018042) similar to phosphatidylinositol glycan, class H (H.
sapiens) [Homo sapiens]
Length = 92
Score = 47.9 bits (113), Expect = 9e-05
Identities = 15/54 (27%), Positives = 25/54 (45%), Gaps = 15/54 (27%)
Query: 20 SYGTQMFALCFISFVIGATSLAIGRSPKIIITLVELSFLLSLFHIISGVNHESL 73
+YG +F LC S ++ A I ITL+ L L H + ++ E++
Sbjct: 52 AYG--LFTLCENSMILSAA---------IFITLLGL---LGYLHFVK-IDQETV 90
>ref|NP_014360.1| (NC_001146) Glysosyl Phosphatidyl Inositol; Gpi15p [Saccharomyces
cerevisiae]
sp|P53961|YND8_YEAST HYPOTHETICAL 24.7 KD PROTEIN IN TFC5-IDH1 INTERGENIC REGION
pir||S62960 probable membrane protein YNL038w - yeast (Saccharomyces
cerevisiae)
emb|CAA95905.1| (Z71314) ORF YNL038w [Saccharomyces cerevisiae]
Length = 212
Score = 38.6 bits (89), Expect = 0.065
Identities = 22/98 (22%), Positives = 47/98 (47%), Gaps = 11/98 (11%)
Query: 49 IITLVELSFLLSLFHIISGVNHESLFVIRDLGVQTNCHSIV---------PWKSSSKLIP 99
I+ ++ L F L ++ G + E++ + ++ G+Q + + + + I
Sbjct: 101 ILIIMGL-FALGTIILVRGPSVETVTIFKESGLQLSRVKGMVIFPQQWNRKFFEQVEFIS 159
Query: 100 LDSIRDIFINEGF-RKFDVCYYMGIAIESETEIHVVFP 136
+ I D+ INEGF R F V +Y+ + + + ++FP
Sbjct: 160 NERIIDVVINEGFCRGFRVIFYLAAIVRKSSTLKLLFP 197
>gb|AAF54173.1| (AE003677) CG14463 gene product [Drosophila melanogaster]
Length = 221
Score = 35.4 bits (81), Expect = 0.59
Identities = 25/112 (22%), Positives = 43/112 (38%), Gaps = 7/112 (6%)
Query: 44 RSPKIIITLVELSFLLSLFHI--ISGVNHESLFVIRDLGVQTNCHSIVPWKSSSKLIPLD 101
P+I+ L+ + + L ++ V E LF D+ +Q + S +
Sbjct: 82 LQPRILYPLITCASVAILLIRSTLNLVQAERLFYSWDMALQMETVRSF-GRESVLCVQRG 140
Query: 102 SIRDIFINEGFRKFDVCYYMGIAIESET----EIHVVFPTLLPRHDVLQKVY 149
I DI +NE DV Y + + + I +F + P + LQ Y
Sbjct: 141 HIEDIVLNEVIEDLDVKYMLILRTKGSQFKKRPIIPLFNSQSPSIECLQHTY 192
CPU time: 43.23 user secs. 3.89 sys. secs 47.12 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.316 0.161 0.452
Gapped
Lambda K H
0.270 0.0564 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88222564
Number of Sequences: 887402
Number of extensions: 3302994
Number of successful extensions: 13249
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 13227
Number of HSP's gapped (non-prelim): 13
length of query: 160
length of database: 277,845,442
effective HSP length: 52
effective length of query: 108
effective length of database: 231,700,538
effective search space: 25023658104
effective search space used: 25023658104
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.9 bits)
S2: 70 (31.4 bits)
