PIG-H: NP_014360.1 BLAST Search Results

GPI Biosynthesis Report

GPI Anchor Biosynthesis Report: NP_014360.1 (PIG-H family, Saccharomyces cerevisiae)

BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (212 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!

 Results of PSI-Blast iteration 1

Distribution of 2 Blast Hits on the Query Sequence

Sequences with E-value BETTER than threshold (0.002)

Sequences producing significant alignments:


 ref|NP_014360.1|  (NC_001146) Glysosyl Phosphatidyl Inositol; Gpi...   347  1e-94

Sequences with E-value WORSE than threshold 

 emb|CAD21200.1|  (AL669999) conserved hypothetical protein [Neuro...    43  0.003

Alignments
>ref|NP_014360.1| (NC_001146) Glysosyl Phosphatidyl Inositol; Gpi15p [Saccharomyces
           cerevisiae]
 sp|P53961|YND8_YEAST HYPOTHETICAL 24.7 KD PROTEIN IN TFC5-IDH1 INTERGENIC REGION
 pir||S62960 probable membrane protein YNL038w - yeast (Saccharomyces
           cerevisiae)
 emb|CAA95905.1| (Z71314) ORF YNL038w [Saccharomyces cerevisiae]
          Length = 212

 Score =  347 bits (882), Expect = 1e-94
 Identities = 212/212 (100%), Positives = 212/212 (100%)

Query: 1   MISKEYEFGKTSILNRKKYTLVIDEDKNGNFIRFTVLPVSNRKFKKVKQNGRVEINMGIQ 60
           MISKEYEFGKTSILNRKKYTLVIDEDKNGNFIRFTVLPVSNRKFKKVKQNGRVEINMGIQ
Sbjct: 1   MISKEYEFGKTSILNRKKYTLVIDEDKNGNFIRFTVLPVSNRKFKKVKQNGRVEINMGIQ 60

Query: 61  YHQIVLILLLNILFYVICLRSRFLEHINRTFEVTIARSFQILIIMGLFALGTIILVRGPS 120
           YHQIVLILLLNILFYVICLRSRFLEHINRTFEVTIARSFQILIIMGLFALGTIILVRGPS
Sbjct: 61  YHQIVLILLLNILFYVICLRSRFLEHINRTFEVTIARSFQILIIMGLFALGTIILVRGPS 120

Query: 121 VETVTIFKESGLQLSRVKGMVIFPQQWNRKFFEQVEFISNERIIDVVINEGFCRGFRVIF 180
           VETVTIFKESGLQLSRVKGMVIFPQQWNRKFFEQVEFISNERIIDVVINEGFCRGFRVIF
Sbjct: 121 VETVTIFKESGLQLSRVKGMVIFPQQWNRKFFEQVEFISNERIIDVVINEGFCRGFRVIF 180

Query: 181 YLAAIVRKSSTLKLLFPVCIQANLRLFLTSTI 212
           YLAAIVRKSSTLKLLFPVCIQANLRLFLTSTI
Sbjct: 181 YLAAIVRKSSTLKLLFPVCIQANLRLFLTSTI 212



>emb|CAD21200.1| (AL669999) conserved hypothetical protein [Neurospora crassa]
          Length = 263

 Score = 43.4 bits (101), Expect = 0.003
 Identities = 31/106 (29%), Positives = 54/106 (50%), Gaps = 3/106 (2%)

Query: 94  TIARSFQILIIMGLFALGTII--LVRGPSVETVTIFKESGLQLSRVKGMVIFPQQWNRKF 151
           ++A S     ++ L AL + I    R  + E++ + +  G+Q+S   G   F +     F
Sbjct: 104 SLAISLPPYAVLALSALTSYIALFARIHTTESLLVLRGLGIQMSSSVGGGNFFRLGGGTF 163

Query: 152 FEQVEFISNERIIDVVINEGFCRGFRVIFYLAAIVRKSSTLKLLFP 197
            ++  FI  E+I D++INE F +GF V +YL  +V     + + FP
Sbjct: 164 MKRTRFIPTEKIQDILINEAF-KGFEVRYYLVIVVEGEQDVVVCFP 208



CPU time:    43.52 user secs.	    1.67 sys. secs	   45.19 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.331    0.146    0.417 

Gapped
Lambda     K      H
   0.270   0.0513    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 114116460
Number of Sequences: 887402
Number of extensions: 4447633
Number of successful extensions: 13171
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 13164
Number of HSP's gapped (non-prelim): 9
length of query: 212
length of database: 277,845,442
effective HSP length: 53
effective length of query: 159
effective length of database: 230,813,136
effective search space: 36699288624
effective search space used: 36699288624
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.9 bits)
S2: 71 (31.9 bits)