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GPI Anchor Biosynthesis Report: CAD21200.1 (PIG-H family, Neurospora crassa)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (263 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!

 Results of PSI-Blast iteration 2

Distribution of 8 Blast Hits on the Query Sequence

Sequences with E-value BETTER than threshold (0.002)


Sequences producing significant alignments:


 emb|CAD21200.1|  (AL669999) conserved hypothetical protein [Neuro...   369  e-101

 pir||T40636  glycosylphosphatidylinositol anchor protein homolog ...   160  2e-38

 ref|NP_004560.1|  (NM_004569) phosphatidylinositol glycan, class ...   155  1e-36

 ref|NP_014360.1|  (NC_001146) Glysosyl Phosphatidyl Inositol; Gpi...   146  3e-34

 ref|NP_195278.1|  (NM_119718) putative protein [Arabidopsis thali...   124  2e-27

 gb|AAL62432.1|  (AY072440) putative protein [Arabidopsis thaliana]     124  2e-27

 dbj|BAB32246.1|  (AK020902) data source:SPTR, source key:Q14442, ...   119  7e-26


Sequences with E-value WORSE than threshold 


 gb|AAF54173.1|  (AE003677) CG14463 gene product [Drosophila melan...    40  0.062


Alignments


>emb|CAD21200.1| (AL669999) conserved hypothetical protein [Neurospora crassa]
          Length = 263

 Score =  369 bits (939), Expect = e-101
 Identities = 254/254 (100%), Positives = 254/254 (100%)

Query: 1   MLTTTPYLTIRRPSPTTAEFTLTTCPPLTLPLRAALFGVLCLRFIAVLSVIIGIYAAFFS 60
           MLTTTPYLTIRRPSPTTAEFTLTTCPPLTLPLRAALFGVLCLRFIAVLSVIIGIYAAFFS
Sbjct: 1   MLTTTPYLTIRRPSPTTAEFTLTTCPPLTLPLRAALFGVLCLRFIAVLSVIIGIYAAFFS 60

Query: 61  PTGLLPPPIFPSGRISFLDFDLNNFLLHILHLLYISRPGQYLASLAISLPPYAVLALSAL 120
           PTGLLPPPIFPSGRISFLDFDLNNFLLHILHLLYISRPGQYLASLAISLPPYAVLALSAL
Sbjct: 61  PTGLLPPPIFPSGRISFLDFDLNNFLLHILHLLYISRPGQYLASLAISLPPYAVLALSAL 120

Query: 121 TSYIALFARIHTTESLLVLRGLGIQMSSSVGGGNFFRLGGGTFMKRTRFIPTEKIQDILI 180
           TSYIALFARIHTTESLLVLRGLGIQMSSSVGGGNFFRLGGGTFMKRTRFIPTEKIQDILI
Sbjct: 121 TSYIALFARIHTTESLLVLRGLGIQMSSSVGGGNFFRLGGGTFMKRTRFIPTEKIQDILI 180

Query: 181 NEAFKGFEVRYYLVIVVEGEQDVVVCFPRLLPRRKIVERVWRGARGCLYEKDGPVLSAGA 240
           NEAFKGFEVRYYLVIVVEGEQDVVVCFPRLLPRRKIVERVWRGARGCLYEKDGPVLSAGA
Sbjct: 181 NEAFKGFEVRYYLVIVVEGEQDVVVCFPRLLPRRKIVERVWRGARGCLYEKDGPVLSAGA 240

Query: 241 GGGGGSHGGNGAWR 254
           GGGGGSHGGNGAWR
Sbjct: 241 GGGGGSHGGNGAWR 254





>pir||T40636 glycosylphosphatidylinositol anchor protein homolog - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAB39362.1| (AL049474) similar to glycosylphosphatidylinositol anchor
           [Schizosaccharomyces pombe]
          Length = 160

 Score =  160 bits (402), Expect = 2e-38
 Identities = 42/121 (34%), Positives = 67/121 (54%), Gaps = 11/121 (9%)

Query: 104 SLAISLPPYAVLALSALTSYIALFARIHTT--ESLLVLRGLGIQMSSSVGGGNFFRLGGG 161
           SLAI   P  ++ L  L+  ++LF  I     ESL V+R LG+Q +              
Sbjct: 39  SLAIGRSPKIIITLVELSFLLSLFHIISGVNHESLFVIRDLGVQTNCH---------SIV 89

Query: 162 TFMKRTRFIPTEKIQDILINEAFKGFEVRYYLVIVVEGEQDVVVCFPRLLPRRKIVERVW 221
            +   ++ IP + I+DI INE F+ F+V YY+ I +E E ++ V FP LLPR  ++++V+
Sbjct: 90  PWKSSSKLIPLDSIRDIFINEGFRKFDVCYYMGIAIESETEIHVVFPTLLPRHDVLQKVY 149

Query: 222 R 222
           +
Sbjct: 150 K 150





>ref|NP_004560.1| (NM_004569) phosphatidylinositol glycan, class H [Homo sapiens]
 pir||A48024 glycosylphosphatidylinositol anchor class H biosynthesis protein -
           human
 gb|AAA03545.1| (L19783) GPI-H [Homo sapiens]
 gb|AAH04100.1|AAH04100 (BC004100) phosphatidylinositol glycan, class H [Homo sapiens]
          Length = 188

 Score =  155 bits (388), Expect = 1e-36
 Identities = 38/125 (30%), Positives = 59/125 (46%), Gaps = 20/125 (16%)

Query: 114 VLALSALTSYIALFARIH----TTESLLVLRGLGIQMSSSVGGGNFFRLGGGTFMKRTRF 169
           +L+ +   + + L   +H      E+LL++  LGIQM+SS   G           + T F
Sbjct: 64  ILSAAIFITLLGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGK----------ESTTF 113

Query: 170 IPTEKIQDILINEAFKGFEVRYYLVIVVE------GEQDVVVCFPRLLPRRKIVERVWRG 223
           I   K++DI+INEA    +V YYL I+++      G   VV  F    PR   +  V+R 
Sbjct: 114 IEMGKVKDIVINEAIYMQKVIYYLCILLKDPVEPHGISQVVPVFQSAKPRLDCLIEVYRS 173

Query: 224 ARGCL 228
            +  L
Sbjct: 174 CQEIL 178





>ref|NP_014360.1| (NC_001146) Glysosyl Phosphatidyl Inositol; Gpi15p [Saccharomyces
           cerevisiae]
 sp|P53961|YND8_YEAST HYPOTHETICAL 24.7 KD PROTEIN IN TFC5-IDH1 INTERGENIC REGION
 pir||S62960 probable membrane protein YNL038w - yeast (Saccharomyces
           cerevisiae)
 emb|CAA95905.1| (Z71314) ORF YNL038w [Saccharomyces cerevisiae]
          Length = 212

 Score =  146 bits (367), Expect = 3e-34
 Identities = 31/106 (29%), Positives = 54/106 (50%), Gaps = 3/106 (2%)

Query: 104 SLAISLPPYAVLALSALTSYIALFARIHTTESLLVLRGLGIQMSSSVGGGNFFRLGGGTF 163
           ++A S     ++ L AL + I    R  + E++ + +  G+Q+S   G   F +     F
Sbjct: 94  TIARSFQILIIMGLFALGTII--LVRGPSVETVTIFKESGLQLSRVKGMVIFPQQWNRKF 151

Query: 164 MKRTRFIPTEKIQDILINEAF-KGFEVRYYLVIVVEGEQDVVVCFP 208
            ++  FI  E+I D++INE F +GF V +YL  +V     + + FP
Sbjct: 152 FEQVEFISNERIIDVVINEGFCRGFRVIFYLAAIVRKSSTLKLLFP 197





>ref|NP_195278.1| (NM_119718) putative protein [Arabidopsis thaliana]
 pir||T04658 hypothetical protein F8D20.40 - Arabidopsis thaliana
 emb|CAA20023.1| (AL031135) putative protein [Arabidopsis thaliana]
 emb|CAB80269.1| (AL161587) putative protein [Arabidopsis thaliana]
          Length = 204

 Score =  124 bits (309), Expect = 2e-27
 Identities = 23/103 (22%), Positives = 44/103 (42%), Gaps = 15/103 (14%)

Query: 125 ALFARIHT-----TESLLVLRGLGIQMSSSVGGGNFFRLGGGTFMKRTRFIPTEKIQDIL 179
                +H+      ES+++L   GIQ+ +    G             +RFIP +KI   +
Sbjct: 88  GFLVMLHSRKFVKKESVIILPTFGIQLETQYLSGKTV----------SRFIPIDKILKPV 137

Query: 180 INEAFKGFEVRYYLVIVVEGEQDVVVCFPRLLPRRKIVERVWR 222
           + E        + L + + GE+ + + F  L P  K++  +W+
Sbjct: 138 LVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWK 180





>gb|AAL62432.1| (AY072440) putative protein [Arabidopsis thaliana]
          Length = 195

 Score =  124 bits (309), Expect = 2e-27
 Identities = 23/103 (22%), Positives = 44/103 (42%), Gaps = 15/103 (14%)

Query: 125 ALFARIHT-----TESLLVLRGLGIQMSSSVGGGNFFRLGGGTFMKRTRFIPTEKIQDIL 179
                +H+      ES+++L   GIQ+ +    G             +RFIP +KI   +
Sbjct: 79  GFLVMLHSRKFVKKESVIILPTFGIQLETQYLSGKTV----------SRFIPIDKILKPV 128

Query: 180 INEAFKGFEVRYYLVIVVEGEQDVVVCFPRLLPRRKIVERVWR 222
           + E        + L + + GE+ + + F  L P  K++  +W+
Sbjct: 129 LVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWK 171





>dbj|BAB32246.1| (AK020902) data source:SPTR, source key:Q14442,
           evidence:ISS~homolog to PHOSPHATIDYLINOSITOL GLYCAN,
           CLASS H~putative [Mus musculus]
          Length = 167

 Score =  119 bits (296), Expect = 7e-26
 Identities = 33/105 (31%), Positives = 51/105 (48%), Gaps = 20/105 (19%)

Query: 114 VLALSALTSYIALFARIH----TTESLLVLRGLGIQMSSSVGGGNFFRLGGGTFMKRTRF 169
           VL+ +   + + L   +H      E+LL++  LGIQM+SS   G           + T F
Sbjct: 64  VLSATIFITILGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGK----------ESTTF 113

Query: 170 IPTEKIQDILINEAFKGFEVRYYLVIVVE------GEQDVVVCFP 208
           I  +K++DI+INEA    +V YYL I+++          VV  F 
Sbjct: 114 IEMDKVKDIIINEAIYMQKVIYYLCILLKEPGKPHEISQVVPVFQ 158





>gb|AAF54173.1| (AE003677) CG14463 gene product [Drosophila melanogaster]
          Length = 221

 Score = 39.7 bits (92), Expect = 0.062
 Identities = 20/115 (17%), Positives = 43/115 (37%), Gaps = 15/115 (13%)

Query: 111 PYAVLALSALTSYIALFARIHTTESLLVLRGLGIQMSSSVGGGNFFRLGGGTFMKRTRFI 170
           P    A  A+    +    +   E L     + +QM +    G           +    +
Sbjct: 89  PLITCASVAILLIRSTLNLVQA-ERLFYSWDMALQMETVRSFGR----------ESVLCV 137

Query: 171 PTEKIQDILINEAFKGFEVRYYLVIVVEGEQ----DVVVCFPRLLPRRKIVERVW 221
               I+DI++NE  +  +V+Y L++  +G Q     ++  F    P  + ++  +
Sbjct: 138 QRGHIEDIVLNEVIEDLDVKYMLILRTKGSQFKKRPIIPLFNSQSPSIECLQHTY 192





CPU time:   102.78 user secs.	    2.10 sys. secs	  104.88 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.323    0.159    0.473 

Gapped
Lambda     K      H
   0.270   0.0558    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 170216215
Number of Sequences: 887402
Number of extensions: 8484219
Number of successful extensions: 138851
Number of sequences better than 10.0: 798
Number of HSP's better than 10.0 without gapping: 626
Number of HSP's successfully gapped in prelim test: 183
Number of HSP's that attempted gapping in prelim test: 94041
Number of HSP's gapped (non-prelim): 22416
length of query: 263
length of database: 277,845,442
effective HSP length: 50
effective length of query: 213
effective length of database: 233,475,342
effective search space: 49730247846
effective search space used: 49730247846
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.3 bits)
S2: 73 (32.6 bits)