Sequences with E-value BETTER than threshold (0.002) 285 3e-76
283 9e-76
166 2e-40
129 3e-29
109 4e-23
Sequences with E-value WORSE than threshold gb|AAL62432.1| (AY072440) putative protein [Arabidopsis thaliana] 40 0.030
ref|NP_195278.1| (NM_119718) putative protein [Arabidopsis thali... 39 0.068
ref|NP_014360.1| (NC_001146) Glysosyl Phosphatidyl Inositol; Gpi... 35 1.0
gb|AAF54173.1| (AE003677) CG14463 gene product [Drosophila melan... 33 3.3
Alignments
>dbj|BAB32246.1| (AK020902) data source:SPTR, source key:Q14442,
evidence:ISS~homolog to PHOSPHATIDYLINOSITOL GLYCAN,
CLASS H~putative [Mus musculus]
Length = 167
Score = 285 bits (723), Expect = 3e-76
Identities = 167/167 (100%), Positives = 167/167 (100%)
Query: 1 MEDEKSFSDICGGRLALRCRYYSPYCREFGLSSARLSLCSLTAVTCAVWLAAYGLFTLCE 60
MEDEKSFSDICGGRLALRCRYYSPYCREFGLSSARLSLCSLTAVTCAVWLAAYGLFTLCE
Sbjct: 1 MEDEKSFSDICGGRLALRCRYYSPYCREFGLSSARLSLCSLTAVTCAVWLAAYGLFTLCE 60
Query: 61 NSMVLSATIFITILGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGKESTTFIEMDKVK 120
NSMVLSATIFITILGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGKESTTFIEMDKVK
Sbjct: 61 NSMVLSATIFITILGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGKESTTFIEMDKVK 120
Query: 121 DIIINEAIYMQKVIYYLCILLKEPGKPHEISQVVPVFQVSTMLSLLL 167
DIIINEAIYMQKVIYYLCILLKEPGKPHEISQVVPVFQVSTMLSLLL
Sbjct: 121 DIIINEAIYMQKVIYYLCILLKEPGKPHEISQVVPVFQVSTMLSLLL 167
>ref|NP_004560.1| (NM_004569) phosphatidylinositol glycan, class H [Homo sapiens]
pir||A48024 glycosylphosphatidylinositol anchor class H biosynthesis protein -
human
gb|AAA03545.1| (L19783) GPI-H [Homo sapiens]
gb|AAH04100.1|AAH04100 (BC004100) phosphatidylinositol glycan, class H [Homo sapiens]
Length = 188
Score = 283 bits (719), Expect = 9e-76
Identities = 140/158 (88%), Positives = 147/158 (92%)
Query: 1 MEDEKSFSDICGGRLALRCRYYSPYCREFGLSSARLSLCSLTAVTCAVWLAAYGLFTLCE 60
MEDE+SFSDICGGRLAL+ RYYSP CREF LS RLSL SLTAVTC VWLAAYGLFTLCE
Sbjct: 1 MEDERSFSDICGGRLALQRRYYSPSCREFCLSCPRLSLRSLTAVTCTVWLAAYGLFTLCE 60
Query: 61 NSMVLSATIFITILGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGKESTTFIEMDKVK 120
NSM+LSA IFIT+LGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGKESTTFIEM KVK
Sbjct: 61 NSMILSAAIFITLLGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGKESTTFIEMGKVK 120
Query: 121 DIIINEAIYMQKVIYYLCILLKEPGKPHEISQVVPVFQ 158
DI+INEAIYMQKVIYYLCILLK+P +PH ISQVVPVFQ
Sbjct: 121 DIVINEAIYMQKVIYYLCILLKDPVEPHGISQVVPVFQ 158
>ref|XP_018042.2| (XM_018042) similar to phosphatidylinositol glycan, class H (H.
sapiens) [Homo sapiens]
Length = 92
Score = 166 bits (417), Expect = 2e-40
Identities = 75/90 (83%), Positives = 80/90 (88%)
Query: 1 MEDEKSFSDICGGRLALRCRYYSPYCREFGLSSARLSLCSLTAVTCAVWLAAYGLFTLCE 60
MEDE+SFSDICGGRLAL+ RYY P CREF LS LSL SLTAVTC VWLAAYGLFTLCE
Sbjct: 1 MEDERSFSDICGGRLALQRRYYFPSCREFCLSCPGLSLRSLTAVTCTVWLAAYGLFTLCE 60
Query: 61 NSMVLSATIFITILGLLGYLHFVKIDQETL 90
NSM+LSA IFIT+LGLLGYLHFVKIDQET+
Sbjct: 61 NSMILSAAIFITLLGLLGYLHFVKIDQETV 90
>pir||T40636 glycosylphosphatidylinositol anchor protein homolog - fission yeast
(Schizosaccharomyces pombe)
emb|CAB39362.1| (AL049474) similar to glycosylphosphatidylinositol anchor
[Schizosaccharomyces pombe]
Length = 160
Score = 129 bits (322), Expect = 3e-29
Identities = 36/113 (31%), Positives = 55/113 (47%), Gaps = 16/113 (14%)
Query: 52 AYG--LFTLCENSMVLSAT---------IFITILG---LLGYLHFVK-IDQETLLIIDSL 96
+YG +F LC S V+ AT I IT++ LL H + ++ E+L +I L
Sbjct: 20 SYGTQMFALCFISFVIGATSLAIGRSPKIIITLVELSFLLSLFHIISGVNHESLFVIRDL 79
Query: 97 GIQMT-SSYASGKESTTFIEMDKVKDIIINEAIYMQKVIYYLCILLKEPGKPH 148
G+Q S K S+ I +D ++DI INE V YY+ I ++ + H
Sbjct: 80 GVQTNCHSIVPWKSSSKLIPLDSIRDIFINEGFRKFDVCYYMGIAIESETEIH 132
>emb|CAD21200.1| (AL669999) conserved hypothetical protein [Neurospora crassa]
Length = 263
Score = 109 bits (270), Expect = 4e-23
Identities = 30/91 (32%), Positives = 48/91 (51%), Gaps = 14/91 (15%)
Query: 64 VLSATIFITILGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGK----------ESTTF 113
VL+ + + + L +H E+LL++ LGIQM+SS G + T F
Sbjct: 114 VLALSALTSYIALFARIH----TTESLLVLRGLGIQMSSSVGGGNFFRLGGGTFMKRTRF 169
Query: 114 IEMDKVKDIIINEAIYMQKVIYYLCILLKEP 144
I +K++DI+INEA +V YYL I+++
Sbjct: 170 IPTEKIQDILINEAFKGFEVRYYLVIVVEGE 200
>gb|AAL62432.1| (AY072440) putative protein [Arabidopsis thaliana]
Length = 195
Score = 39.7 bits (92), Expect = 0.030
Identities = 23/73 (31%), Positives = 43/73 (58%), Gaps = 1/73 (1%)
Query: 48 VWLAAYGLFTLCENSMVLSATIFITILGLLGYLHFVK-IDQETLLIIDSLGIQMTSSYAS 106
V+LA+ F L +++ + + + G L LH K + +E+++I+ + GIQ+ + Y S
Sbjct: 52 VFLASSMYFLLGKDNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLS 111
Query: 107 GKESTTFIEMDKV 119
GK + FI +DK+
Sbjct: 112 GKTVSRFIPIDKI 124
>ref|NP_195278.1| (NM_119718) putative protein [Arabidopsis thaliana]
pir||T04658 hypothetical protein F8D20.40 - Arabidopsis thaliana
emb|CAA20023.1| (AL031135) putative protein [Arabidopsis thaliana]
emb|CAB80269.1| (AL161587) putative protein [Arabidopsis thaliana]
Length = 204
Score = 38.6 bits (89), Expect = 0.068
Identities = 18/46 (39%), Positives = 30/46 (65%), Gaps = 1/46 (2%)
Query: 75 GLLGYLHFVK-IDQETLLIIDSLGIQMTSSYASGKESTTFIEMDKV 119
G L LH K + +E+++I+ + GIQ+ + Y SGK + FI +DK+
Sbjct: 88 GFLVMLHSRKFVKKESVIILPTFGIQLETQYLSGKTVSRFIPIDKI 133
>ref|NP_014360.1| (NC_001146) Glysosyl Phosphatidyl Inositol; Gpi15p [Saccharomyces
cerevisiae]
sp|P53961|YND8_YEAST HYPOTHETICAL 24.7 KD PROTEIN IN TFC5-IDH1 INTERGENIC REGION
pir||S62960 probable membrane protein YNL038w - yeast (Saccharomyces
cerevisiae)
emb|CAA95905.1| (Z71314) ORF YNL038w [Saccharomyces cerevisiae]
Length = 212
Score = 34.7 bits (79), Expect = 1.0
Identities = 18/84 (21%), Positives = 34/84 (40%), Gaps = 11/84 (13%)
Query: 69 IFITILGLLGYLHFVKIDQETLLIIDSLGIQMTSSYA------SGK----ESTTFIEMDK 118
I + + L + ET+ I G+Q++ E FI ++
Sbjct: 103 IIMGLFALGTIILVRGPSVETVTIFKESGLQLSRVKGMVIFPQQWNRKFFEQVEFISNER 162
Query: 119 VKDIIINEAI-YMQKVIYYLCILL 141
+ D++INE +VI+YL ++
Sbjct: 163 IIDVVINEGFCRGFRVIFYLAAIV 186
>gb|AAF54173.1| (AE003677) CG14463 gene product [Drosophila melanogaster]
Length = 221
Score = 33.1 bits (75), Expect = 3.3
Identities = 21/82 (25%), Positives = 39/82 (46%), Gaps = 2/82 (2%)
Query: 76 LLGYLHFVKIDQETLLIIDSLGIQMTSSYASGKESTTFIEMDKVKDIIINEAIYMQKVIY 135
LL + E L + +QM + + G+ES ++ ++DI++NE I V Y
Sbjct: 99 LLIRSTLNLVQAERLFYSWDMALQMETVRSFGRESVLCVQRGHIEDIVLNEVIEDLDVKY 158
Query: 136 YLCILLKEPGKPHEISQVVPVF 157
L +L+ G + ++P+F
Sbjct: 159 ML--ILRTKGSQFKKRPIIPLF 178
CPU time: 38.69 user secs. 1.62 sys. secs 40.31 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.325 0.160 0.492
Gapped
Lambda K H
0.270 0.0562 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93181111
Number of Sequences: 887402
Number of extensions: 3659624
Number of successful extensions: 12396
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 12382
Number of HSP's gapped (non-prelim): 16
length of query: 167
length of database: 277,845,442
effective HSP length: 53
effective length of query: 114
effective length of database: 230,813,136
effective search space: 26312697504
effective search space used: 26312697504
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.4 bits)
S2: 70 (31.4 bits)
