Sequences with E-value BETTER than threshold (0.002)
Sequences producing significant alignments:
gb|AAL62432.1| (AY072440) putative protein [Arabidopsis thaliana] 303 2e-81
ref|NP_195278.1| (NM_119718) putative protein [Arabidopsis thali... 294 7e-79
ref|NP_004560.1| (NM_004569) phosphatidylinositol glycan, class ... 194 8e-49
ref|NP_014360.1| (NC_001146) Glysosyl Phosphatidyl Inositol; Gpi... 158 6e-38
pir||T40636 glycosylphosphatidylinositol anchor protein homolog ... 156 2e-37
emb|CAD21200.1| (AL669999) conserved hypothetical protein [Neuro... 155 6e-37
dbj|BAB32246.1| (AK020902) data source:SPTR, source key:Q14442, ... 155 6e-37
ref|XP_018042.2| (XM_018042) similar to phosphatidylinositol gly... 69 7e-11
Sequences with E-value WORSE than threshold
gb|AAF54173.1| (AE003677) CG14463 gene product [Drosophila melan... 37 0.20
Alignments
>gb|AAL62432.1| (AY072440) putative protein [Arabidopsis thaliana]
Length = 195
Score = 303 bits (769), Expect = 2e-81
Identities = 195/195 (100%), Positives = 195/195 (100%)
Query: 1 MVSLSVSNKRYTYIHESGSKSTREAIDIHHVIINGSSGTGYARRWGLGFFLVFLASSMYF 60
MVSLSVSNKRYTYIHESGSKSTREAIDIHHVIINGSSGTGYARRWGLGFFLVFLASSMYF
Sbjct: 1 MVSLSVSNKRYTYIHESGSKSTREAIDIHHVIINGSSGTGYARRWGLGFFLVFLASSMYF 60
Query: 61 LLGKDNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLSGKTVSRFIP 120
LLGKDNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLSGKTVSRFIP
Sbjct: 61 LLGKDNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLSGKTVSRFIP 120
Query: 121 IDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWKALCAAIGTD 180
IDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWKALCAAIGTD
Sbjct: 121 IDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWKALCAAIGTD 180
Query: 181 HQSETIAEEEHDVSS 195
HQSETIAEEEHDVSS
Sbjct: 181 HQSETIAEEEHDVSS 195
>ref|NP_195278.1| (NM_119718) putative protein [Arabidopsis thaliana]
pir||T04658 hypothetical protein F8D20.40 - Arabidopsis thaliana
emb|CAA20023.1| (AL031135) putative protein [Arabidopsis thaliana]
emb|CAB80269.1| (AL161587) putative protein [Arabidopsis thaliana]
Length = 204
Score = 294 bits (746), Expect = 7e-79
Identities = 195/204 (95%), Positives = 195/204 (95%), Gaps = 9/204 (4%)
Query: 1 MVSLSVSNKRYTYIHESGSKSTREAIDIHHVIINGSSGTGYARRWGLGFFLVFLASSMYF 60
MVSLSVSNKRYTYIHESGSKSTREAIDIHHVIINGSSGTGYARRWGLGFFLVFLASSMYF
Sbjct: 1 MVSLSVSNKRYTYIHESGSKSTREAIDIHHVIINGSSGTGYARRWGLGFFLVFLASSMYF 60
Query: 61 LLGK---------DNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLS 111
LLGK DNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLS
Sbjct: 61 LLGKLKGIIHMKQDNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLS 120
Query: 112 GKTVSRFIPIDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWK 171
GKTVSRFIPIDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWK
Sbjct: 121 GKTVSRFIPIDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWK 180
Query: 172 ALCAAIGTDHQSETIAEEEHDVSS 195
ALCAAIGTDHQSETIAEEEHDVSS
Sbjct: 181 ALCAAIGTDHQSETIAEEEHDVSS 204
>ref|NP_004560.1| (NM_004569) phosphatidylinositol glycan, class H [Homo sapiens]
pir||A48024 glycosylphosphatidylinositol anchor class H biosynthesis protein -
human
gb|AAA03545.1| (L19783) GPI-H [Homo sapiens]
gb|AAH04100.1|AAH04100 (BC004100) phosphatidylinositol glycan, class H [Homo sapiens]
Length = 188
Score = 194 bits (490), Expect = 8e-49
Identities = 39/140 (27%), Positives = 71/140 (49%), Gaps = 9/140 (6%)
Query: 52 VFLASSMYFLLGKDNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLS 111
V+LA+ F L +++ + + L G L LH K + +E+++I+ + GIQ+ + Y S
Sbjct: 48 VWLAAYGLFTLCENSMILSAAIFITLLGLLGYLHFVK-IDQETLLIIDSLGIQMTSSYAS 106
Query: 112 GKTVSRFIPIDKILKPVLVECVTPITCYWSLSLFLR------GEEQLTLVFKELRPPLKM 165
GK + FI + K+ V+ E + + L + L+ G Q+ VF+ +P L
Sbjct: 107 GKESTTFIEMGKVKDIVINEAIYMQKVIYYLCILLKDPVEPHGISQVVPVFQSAKPRLDC 166
Query: 166 LVPIWKALCAAIGTDHQSET 185
L+ ++++ C I HQ T
Sbjct: 167 LIEVYRS-CQEI-LAHQKAT 184
>ref|NP_014360.1| (NC_001146) Glysosyl Phosphatidyl Inositol; Gpi15p [Saccharomyces
cerevisiae]
sp|P53961|YND8_YEAST HYPOTHETICAL 24.7 KD PROTEIN IN TFC5-IDH1 INTERGENIC REGION
pir||S62960 probable membrane protein YNL038w - yeast (Saccharomyces
cerevisiae)
emb|CAA95905.1| (Z71314) ORF YNL038w [Saccharomyces cerevisiae]
Length = 212
Score = 158 bits (397), Expect = 6e-38
Identities = 29/169 (17%), Positives = 58/169 (34%), Gaps = 23/169 (13%)
Query: 4 LSVSNKRYTYIHESGSKSTREAIDIHHVIINGSSGTGYARRWGLGFFLVFLASSMYFLLG 63
L VSN+++ + ++G I H +++ F+++ L S +
Sbjct: 37 LPVSNRKFKKVKQNGRVEINMGIQYHQIVLILLLNI--------LFYVICLRSRFLEHIN 88
Query: 64 KD---NPARTLSWGCLL-SGFLVMLHSRKFVKKESVIILPTFGIQLETQYL------SGK 113
+ AR+ ++ L + + E+V I G+QL
Sbjct: 89 RTFEVTIARSFQILIIMGLFALGTIILVRGPSVETVTIFKESGLQLSRVKGMVIFPQQWN 148
Query: 114 ----TVSRFIPIDKILKPVLVECV-TPITCYWSLSLFLRGEEQLTLVFK 157
FI ++I+ V+ E + L+ +R L L+F
Sbjct: 149 RKFFEQVEFISNERIIDVVINEGFCRGFRVIFYLAAIVRKSSTLKLLFP 197
>pir||T40636 glycosylphosphatidylinositol anchor protein homolog - fission yeast
(Schizosaccharomyces pombe)
emb|CAB39362.1| (AL049474) similar to glycosylphosphatidylinositol anchor
[Schizosaccharomyces pombe]
Length = 160
Score = 156 bits (393), Expect = 2e-37
Identities = 28/125 (22%), Positives = 53/125 (42%), Gaps = 6/125 (4%)
Query: 53 FLASSMYFLLGKDNPA--RTLSWGCL---LSGFLVMLHSRKFVKKESVIILPTFGIQLET 107
F + F++G + A R+ LS L + H V ES+ ++ G+Q
Sbjct: 26 FALCFISFVIGATSLAIGRSPKIIITLVELSFLLSLFHIISGVNHESLFVIRDLGVQTNC 85
Query: 108 -QYLSGKTVSRFIPIDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKML 166
+ K+ S+ IP+D I + E + + + + E ++ +VF L P +L
Sbjct: 86 HSIVPWKSSSKLIPLDSIRDIFINEGFRKFDVCYYMGIAIESETEIHVVFPTLLPRHDVL 145
Query: 167 VPIWK 171
++K
Sbjct: 146 QKVYK 150
>emb|CAD21200.1| (AL669999) conserved hypothetical protein [Neurospora crassa]
Length = 263
Score = 155 bits (389), Expect = 6e-37
Identities = 25/121 (20%), Positives = 47/121 (38%), Gaps = 11/121 (9%)
Query: 62 LGKDNPARTLSWGCLLSGFLVMLH-SRKFVKKESVIILPTFGIQLETQYLSGK------- 113
L + LS + + ES+++L GIQ+ + G
Sbjct: 102 LASLAISLPPYAVLALSALTSYIALFARIHTTESLLVLRGLGIQMSSSVGGGNFFRLGGG 161
Query: 114 ---TVSRFIPIDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIW 170
+RFIP +KI ++ E + L + + GE+ + + F L P K++ +W
Sbjct: 162 TFMKRTRFIPTEKIQDILINEAFKGFEVRYYLVIVVEGEQDVVVCFPRLLPRRKIVERVW 221
Query: 171 K 171
+
Sbjct: 222 R 222
>dbj|BAB32246.1| (AK020902) data source:SPTR, source key:Q14442,
evidence:ISS~homolog to PHOSPHATIDYLINOSITOL GLYCAN,
CLASS H~putative [Mus musculus]
Length = 167
Score = 155 bits (389), Expect = 6e-37
Identities = 29/112 (25%), Positives = 57/112 (50%), Gaps = 7/112 (6%)
Query: 52 VFLASSMYFLLGKDNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLS 111
V+LA+ F L +++ + + + G L LH K + +E+++I+ + GIQ+ + Y S
Sbjct: 48 VWLAAYGLFTLCENSMVLSATIFITILGLLGYLHFVK-IDQETLLIIDSLGIQMTSSYAS 106
Query: 112 GKTVSRFIPIDKILKPVLVECVTPITCYWSLSLFLR------GEEQLTLVFK 157
GK + FI +DK+ ++ E + + L + L+ Q+ VF+
Sbjct: 107 GKESTTFIEMDKVKDIIINEAIYMQKVIYYLCILLKEPGKPHEISQVVPVFQ 158
>ref|XP_018042.2| (XM_018042) similar to phosphatidylinositol glycan, class H (H.
sapiens) [Homo sapiens]
Length = 92
Score = 68.5 bits (166), Expect = 7e-11
Identities = 13/44 (29%), Positives = 23/44 (51%), Gaps = 1/44 (2%)
Query: 52 VFLASSMYFLLGKDNPARTLSWGCLLSGFLVMLHSRKFVKKESV 95
V+LA+ F L +++ + + L G L LH K + +E+V
Sbjct: 48 VWLAAYGLFTLCENSMILSAAIFITLLGLLGYLHFVK-IDQETV 90
>gb|AAF54173.1| (AE003677) CG14463 gene product [Drosophila melanogaster]
Length = 221
Score = 37.3 bits (86), Expect = 0.20
Identities = 31/180 (17%), Positives = 59/180 (32%), Gaps = 21/180 (11%)
Query: 15 HESGSKSTREAIDIHHV--------IINGSSGTGYARRWGLGFFLVFLA-SSMYFLLGKD 65
G+ + I H ++N G R+ L+FL+ + G D
Sbjct: 16 KYEGAVEADLELCIKHCGGDVVRIELVNRLHGIQLQRKLRRLLLLMFLSIAYFVGTCGFD 75
Query: 66 NPART--------LSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLSGKTVSR 117
N + C L++ + V+ E + +Q+ET G+
Sbjct: 76 NRTISFLQPRILYPLITCASVAILLIRSTLNLVQAERLFYSWDMALQMETVRSFGRESVL 135
Query: 118 FIPIDKILKPVLVECVTPITCYWSLSLFLRGEE----QLTLVFKELRPPLKMLVPIWKAL 173
+ I VL E + + + L L +G + + +F P ++ L + L
Sbjct: 136 CVQRGHIEDIVLNEVIEDLDVKYMLILRTKGSQFKKRPIIPLFNSQSPSIECLQHTYHVL 195
CPU time: 42.03 user secs. 1.66 sys. secs 43.69 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.317 0.166 0.464
Gapped
Lambda K H
0.270 0.0581 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 105942927
Number of Sequences: 887402
Number of extensions: 4250506
Number of successful extensions: 13958
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 13930
Number of HSP's gapped (non-prelim): 26
length of query: 195
length of database: 277,845,442
effective HSP length: 53
effective length of query: 142
effective length of database: 230,813,136
effective search space: 32775465312
effective search space used: 32775465312
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.3 bits)
S2: 71 (31.8 bits)