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GPI Anchor Biosynthesis Report: AAL62432.1 (PIG-H family, Arabidopsis thaliana)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (195 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 3

Distribution of 9 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)
Sequences producing significant alignments:
gb|AAL62432.1| (AY072440) putative protein [Arabidopsis thaliana] 303 2e-81
ref|NP_195278.1| (NM_119718) putative protein [Arabidopsis thali... 294 7e-79
ref|NP_004560.1| (NM_004569) phosphatidylinositol glycan, class ... 194 8e-49
ref|NP_014360.1| (NC_001146) Glysosyl Phosphatidyl Inositol; Gpi... 158 6e-38
pir||T40636 glycosylphosphatidylinositol anchor protein homolog ... 156 2e-37
emb|CAD21200.1| (AL669999) conserved hypothetical protein [Neuro... 155 6e-37
dbj|BAB32246.1| (AK020902) data source:SPTR, source key:Q14442, ... 155 6e-37
ref|XP_018042.2| (XM_018042) similar to phosphatidylinositol gly... 69 7e-11
Sequences with E-value WORSE than threshold

gb|AAF54173.1| (AE003677) CG14463 gene product [Drosophila melan... 37 0.20
Alignments
>gb|AAL62432.1| (AY072440) putative protein [Arabidopsis thaliana]
          Length = 195

 Score =  303 bits (769), Expect = 2e-81
 Identities = 195/195 (100%), Positives = 195/195 (100%)

Query: 1   MVSLSVSNKRYTYIHESGSKSTREAIDIHHVIINGSSGTGYARRWGLGFFLVFLASSMYF 60
           MVSLSVSNKRYTYIHESGSKSTREAIDIHHVIINGSSGTGYARRWGLGFFLVFLASSMYF
Sbjct: 1   MVSLSVSNKRYTYIHESGSKSTREAIDIHHVIINGSSGTGYARRWGLGFFLVFLASSMYF 60

Query: 61  LLGKDNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLSGKTVSRFIP 120
           LLGKDNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLSGKTVSRFIP
Sbjct: 61  LLGKDNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLSGKTVSRFIP 120

Query: 121 IDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWKALCAAIGTD 180
           IDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWKALCAAIGTD
Sbjct: 121 IDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWKALCAAIGTD 180

Query: 181 HQSETIAEEEHDVSS 195
           HQSETIAEEEHDVSS
Sbjct: 181 HQSETIAEEEHDVSS 195
>ref|NP_195278.1| (NM_119718) putative protein [Arabidopsis thaliana]
 pir||T04658 hypothetical protein F8D20.40 - Arabidopsis thaliana
 emb|CAA20023.1| (AL031135) putative protein [Arabidopsis thaliana]
 emb|CAB80269.1| (AL161587) putative protein [Arabidopsis thaliana]
          Length = 204

 Score =  294 bits (746), Expect = 7e-79
 Identities = 195/204 (95%), Positives = 195/204 (95%), Gaps = 9/204 (4%)

Query: 1   MVSLSVSNKRYTYIHESGSKSTREAIDIHHVIINGSSGTGYARRWGLGFFLVFLASSMYF 60
           MVSLSVSNKRYTYIHESGSKSTREAIDIHHVIINGSSGTGYARRWGLGFFLVFLASSMYF
Sbjct: 1   MVSLSVSNKRYTYIHESGSKSTREAIDIHHVIINGSSGTGYARRWGLGFFLVFLASSMYF 60

Query: 61  LLGK---------DNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLS 111
           LLGK         DNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLS
Sbjct: 61  LLGKLKGIIHMKQDNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLS 120

Query: 112 GKTVSRFIPIDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWK 171
           GKTVSRFIPIDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWK
Sbjct: 121 GKTVSRFIPIDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIWK 180

Query: 172 ALCAAIGTDHQSETIAEEEHDVSS 195
           ALCAAIGTDHQSETIAEEEHDVSS
Sbjct: 181 ALCAAIGTDHQSETIAEEEHDVSS 204
>ref|NP_004560.1| (NM_004569) phosphatidylinositol glycan, class H [Homo sapiens]
 pir||A48024 glycosylphosphatidylinositol anchor class H biosynthesis protein -
           human
 gb|AAA03545.1| (L19783) GPI-H [Homo sapiens]
 gb|AAH04100.1|AAH04100 (BC004100) phosphatidylinositol glycan, class H [Homo sapiens]
          Length = 188

 Score =  194 bits (490), Expect = 8e-49
 Identities = 39/140 (27%), Positives = 71/140 (49%), Gaps = 9/140 (6%)

Query: 52  VFLASSMYFLLGKDNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLS 111
           V+LA+   F L +++   + +    L G L  LH  K + +E+++I+ + GIQ+ + Y S
Sbjct: 48  VWLAAYGLFTLCENSMILSAAIFITLLGLLGYLHFVK-IDQETLLIIDSLGIQMTSSYAS 106

Query: 112 GKTVSRFIPIDKILKPVLVECVTPITCYWSLSLFLR------GEEQLTLVFKELRPPLKM 165
           GK  + FI + K+   V+ E +      + L + L+      G  Q+  VF+  +P L  
Sbjct: 107 GKESTTFIEMGKVKDIVINEAIYMQKVIYYLCILLKDPVEPHGISQVVPVFQSAKPRLDC 166

Query: 166 LVPIWKALCAAIGTDHQSET 185
           L+ ++++ C  I   HQ  T
Sbjct: 167 LIEVYRS-CQEI-LAHQKAT 184
>ref|NP_014360.1| (NC_001146) Glysosyl Phosphatidyl Inositol; Gpi15p [Saccharomyces
           cerevisiae]
 sp|P53961|YND8_YEAST HYPOTHETICAL 24.7 KD PROTEIN IN TFC5-IDH1 INTERGENIC REGION
 pir||S62960 probable membrane protein YNL038w - yeast (Saccharomyces
           cerevisiae)
 emb|CAA95905.1| (Z71314) ORF YNL038w [Saccharomyces cerevisiae]
          Length = 212

 Score =  158 bits (397), Expect = 6e-38
 Identities = 29/169 (17%), Positives = 58/169 (34%), Gaps = 23/169 (13%)

Query: 4   LSVSNKRYTYIHESGSKSTREAIDIHHVIINGSSGTGYARRWGLGFFLVFLASSMYFLLG 63
           L VSN+++  + ++G       I  H +++               F+++ L S     + 
Sbjct: 37  LPVSNRKFKKVKQNGRVEINMGIQYHQIVLILLLNI--------LFYVICLRSRFLEHIN 88

Query: 64  KD---NPARTLSWGCLL-SGFLVMLHSRKFVKKESVIILPTFGIQLETQYL------SGK 113
           +      AR+     ++    L  +   +    E+V I    G+QL              
Sbjct: 89  RTFEVTIARSFQILIIMGLFALGTIILVRGPSVETVTIFKESGLQLSRVKGMVIFPQQWN 148

Query: 114 ----TVSRFIPIDKILKPVLVECV-TPITCYWSLSLFLRGEEQLTLVFK 157
                   FI  ++I+  V+ E         + L+  +R    L L+F 
Sbjct: 149 RKFFEQVEFISNERIIDVVINEGFCRGFRVIFYLAAIVRKSSTLKLLFP 197
>pir||T40636 glycosylphosphatidylinositol anchor protein homolog - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAB39362.1| (AL049474) similar to glycosylphosphatidylinositol anchor
           [Schizosaccharomyces pombe]
          Length = 160

 Score =  156 bits (393), Expect = 2e-37
 Identities = 28/125 (22%), Positives = 53/125 (42%), Gaps = 6/125 (4%)

Query: 53  FLASSMYFLLGKDNPA--RTLSWGCL---LSGFLVMLHSRKFVKKESVIILPTFGIQLET 107
           F    + F++G  + A  R+         LS  L + H    V  ES+ ++   G+Q   
Sbjct: 26  FALCFISFVIGATSLAIGRSPKIIITLVELSFLLSLFHIISGVNHESLFVIRDLGVQTNC 85

Query: 108 -QYLSGKTVSRFIPIDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKML 166
              +  K+ S+ IP+D I    + E        + + + +  E ++ +VF  L P   +L
Sbjct: 86  HSIVPWKSSSKLIPLDSIRDIFINEGFRKFDVCYYMGIAIESETEIHVVFPTLLPRHDVL 145

Query: 167 VPIWK 171
             ++K
Sbjct: 146 QKVYK 150
>emb|CAD21200.1| (AL669999) conserved hypothetical protein [Neurospora crassa]
          Length = 263

 Score =  155 bits (389), Expect = 6e-37
 Identities = 25/121 (20%), Positives = 47/121 (38%), Gaps = 11/121 (9%)

Query: 62  LGKDNPARTLSWGCLLSGFLVMLH-SRKFVKKESVIILPTFGIQLETQYLSGK------- 113
           L     +        LS     +    +    ES+++L   GIQ+ +    G        
Sbjct: 102 LASLAISLPPYAVLALSALTSYIALFARIHTTESLLVLRGLGIQMSSSVGGGNFFRLGGG 161

Query: 114 ---TVSRFIPIDKILKPVLVECVTPITCYWSLSLFLRGEEQLTLVFKELRPPLKMLVPIW 170
                +RFIP +KI   ++ E        + L + + GE+ + + F  L P  K++  +W
Sbjct: 162 TFMKRTRFIPTEKIQDILINEAFKGFEVRYYLVIVVEGEQDVVVCFPRLLPRRKIVERVW 221

Query: 171 K 171
           +
Sbjct: 222 R 222
>dbj|BAB32246.1| (AK020902) data source:SPTR, source key:Q14442,
           evidence:ISS~homolog to PHOSPHATIDYLINOSITOL GLYCAN,
           CLASS H~putative [Mus musculus]
          Length = 167

 Score =  155 bits (389), Expect = 6e-37
 Identities = 29/112 (25%), Positives = 57/112 (50%), Gaps = 7/112 (6%)

Query: 52  VFLASSMYFLLGKDNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLS 111
           V+LA+   F L +++   + +    + G L  LH  K + +E+++I+ + GIQ+ + Y S
Sbjct: 48  VWLAAYGLFTLCENSMVLSATIFITILGLLGYLHFVK-IDQETLLIIDSLGIQMTSSYAS 106

Query: 112 GKTVSRFIPIDKILKPVLVECVTPITCYWSLSLFLR------GEEQLTLVFK 157
           GK  + FI +DK+   ++ E +      + L + L+         Q+  VF+
Sbjct: 107 GKESTTFIEMDKVKDIIINEAIYMQKVIYYLCILLKEPGKPHEISQVVPVFQ 158
>ref|XP_018042.2| (XM_018042) similar to phosphatidylinositol glycan, class H (H.
          sapiens) [Homo sapiens]
          Length = 92

 Score = 68.5 bits (166), Expect = 7e-11
 Identities = 13/44 (29%), Positives = 23/44 (51%), Gaps = 1/44 (2%)

Query: 52 VFLASSMYFLLGKDNPARTLSWGCLLSGFLVMLHSRKFVKKESV 95
          V+LA+   F L +++   + +    L G L  LH  K + +E+V
Sbjct: 48 VWLAAYGLFTLCENSMILSAAIFITLLGLLGYLHFVK-IDQETV 90
>gb|AAF54173.1| (AE003677) CG14463 gene product [Drosophila melanogaster]
          Length = 221

 Score = 37.3 bits (86), Expect = 0.20
 Identities = 31/180 (17%), Positives = 59/180 (32%), Gaps = 21/180 (11%)

Query: 15  HESGSKSTREAIDIHHV--------IINGSSGTGYARRWGLGFFLVFLA-SSMYFLLGKD 65
              G+      + I H         ++N   G    R+      L+FL+ +      G D
Sbjct: 16  KYEGAVEADLELCIKHCGGDVVRIELVNRLHGIQLQRKLRRLLLLMFLSIAYFVGTCGFD 75

Query: 66  NPART--------LSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLSGKTVSR 117
           N   +            C     L++  +   V+ E +       +Q+ET    G+    
Sbjct: 76  NRTISFLQPRILYPLITCASVAILLIRSTLNLVQAERLFYSWDMALQMETVRSFGRESVL 135

Query: 118 FIPIDKILKPVLVECVTPITCYWSLSLFLRGEE----QLTLVFKELRPPLKMLVPIWKAL 173
            +    I   VL E +  +   + L L  +G +     +  +F    P ++ L   +  L
Sbjct: 136 CVQRGHIEDIVLNEVIEDLDVKYMLILRTKGSQFKKRPIIPLFNSQSPSIECLQHTYHVL 195
CPU time:    42.03 user secs.	    1.66 sys. secs	   43.69 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.317    0.166    0.464 

Gapped
Lambda     K      H
   0.270   0.0581    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 105942927
Number of Sequences: 887402
Number of extensions: 4250506
Number of successful extensions: 13958
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 13930
Number of HSP's gapped (non-prelim): 26
length of query: 195
length of database: 277,845,442
effective HSP length: 53
effective length of query: 142
effective length of database: 230,813,136
effective search space: 32775465312
effective search space used: 32775465312
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.3 bits)
S2: 71 (31.8 bits)