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GPI Anchor Biosynthesis Report: AAF54173.1 (PIG-H family, Drosophila melanogaster)




BLASTP 2.1.1 [Aug-8-2000]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (221 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!

 Results of PSI-Blast iteration 1

Distribution of 3 Blast Hits on the Query Sequence

Sequences with E-value BETTER than threshold (0.002)



 gb|AAF54173.1|  (AE003677) CG14463 gene product [Drosophila melan...   414  e-115


Sequences with E-value WORSE than threshold 


 ref|NP_004560.1|  (NM_004569) phosphatidylinositol glycan, class ...    42  0.008

 dbj|BAB32246.1|  (AK020902) data source:SPTR, source key:Q14442, ...    32  9.0


Alignments


>gb|AAF54173.1| (AE003677) CG14463 gene product [Drosophila melanogaster]
          Length = 221

 Score =  414 bits (1054), Expect = e-115
 Identities = 221/221 (100%), Positives = 221/221 (100%)

Query: 1   MEPKVTIHRYYSRQAKYEGAVEADLELCIKHCGGDVVRIELVNRLHGIQLQRKLRRLLLL 60
           MEPKVTIHRYYSRQAKYEGAVEADLELCIKHCGGDVVRIELVNRLHGIQLQRKLRRLLLL
Sbjct: 1   MEPKVTIHRYYSRQAKYEGAVEADLELCIKHCGGDVVRIELVNRLHGIQLQRKLRRLLLL 60

Query: 61  MFLSIAYFVGTCGFDNRTISFLQPRILYPLITCASVAILLIRSTLNLVQAERLFYSWDMA 120
           MFLSIAYFVGTCGFDNRTISFLQPRILYPLITCASVAILLIRSTLNLVQAERLFYSWDMA
Sbjct: 61  MFLSIAYFVGTCGFDNRTISFLQPRILYPLITCASVAILLIRSTLNLVQAERLFYSWDMA 120

Query: 121 LQMETVRSFGRESVLCVQRGHIEDIVLNEVIEDLDVKYMLILRTKGSQFKKRPIIPLFNS 180
           LQMETVRSFGRESVLCVQRGHIEDIVLNEVIEDLDVKYMLILRTKGSQFKKRPIIPLFNS
Sbjct: 121 LQMETVRSFGRESVLCVQRGHIEDIVLNEVIEDLDVKYMLILRTKGSQFKKRPIIPLFNS 180

Query: 181 QSPSIECLQHTYHVLHKYWLNSTKDRSEESYSKDKQSLVNS 221
           QSPSIECLQHTYHVLHKYWLNSTKDRSEESYSKDKQSLVNS
Sbjct: 181 QSPSIECLQHTYHVLHKYWLNSTKDRSEESYSKDKQSLVNS 221





>ref|NP_004560.1| (NM_004569) phosphatidylinositol glycan, class H [Homo sapiens]
 pir||A48024 glycosylphosphatidylinositol anchor class H biosynthesis protein -
           human
 gb|AAA03545.1| (L19783) GPI-H [Homo sapiens]
 gb|AAH04100.1|AAH04100 (BC004100) phosphatidylinositol glycan, class H [Homo sapiens]
          Length = 188

 Score = 42.3 bits (98), Expect = 0.008
 Identities = 25/87 (28%), Positives = 43/87 (48%), Gaps = 2/87 (2%)

Query: 108 VQAERLFYSWDMALQMETVRSFGRESVLCVQRGHIEDIVLNEVIEDLDVKYML--ILRTK 165
           +  E L     + +QM +  + G+ES   ++ G ++DIV+NE I    V Y L  +L+  
Sbjct: 85  IDQETLLIIDSLGIQMTSSYASGKESTTFIEMGKVKDIVINEAIYMQKVIYYLCILLKDP 144

Query: 166 GSQFKKRPIIPLFNSQSPSIECLQHTY 192
                   ++P+F S  P ++CL   Y
Sbjct: 145 VEPHGISQVVPVFQSAKPRLDCLIEVY 171





>dbj|BAB32246.1| (AK020902) data source:SPTR, source key:Q14442,
           evidence:ISS~homolog to PHOSPHATIDYLINOSITOL GLYCAN,
           CLASS H~putative [Mus musculus]
          Length = 167

 Score = 32.1 bits (72), Expect = 9.0
 Identities = 19/73 (26%), Positives = 37/73 (50%), Gaps = 2/73 (2%)

Query: 108 VQAERLFYSWDMALQMETVRSFGRESVLCVQRGHIEDIVLNEVIEDLDVKYML--ILRTK 165
           +  E L     + +QM +  + G+ES   ++   ++DI++NE I    V Y L  +L+  
Sbjct: 85  IDQETLLIIDSLGIQMTSSYASGKESTTFIEMDKVKDIIINEAIYMQKVIYYLCILLKEP 144

Query: 166 GSQFKKRPIIPLF 178
           G   +   ++P+F
Sbjct: 145 GKPHEISQVVPVF 157





CPU time:    46.15 user secs.	    1.61 sys. secs	   47.76 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.325    0.140    0.411 

Gapped
Lambda     K      H
   0.270   0.0490    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 125003498
Number of Sequences: 887402
Number of extensions: 4760881
Number of successful extensions: 14676
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 14672
Number of HSP's gapped (non-prelim): 8
length of query: 221
length of database: 277,845,442
effective HSP length: 54
effective length of query: 167
effective length of database: 229,925,734
effective search space: 38397597578
effective search space used: 38397597578
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.6 bits)
S2: 71 (32.0 bits)