Sequences with E-value BETTER than threshold (0.002) 269 3e-71
242 5e-63
239 5e-62
185 5e-46
185 6e-46
158 1e-37
144 1e-33
144 2e-33
126 4e-28
126 5e-28
102 6e-21
87 2e-16
Alignments
>gb|AAL48412.1| (AY070790) AT13969p [Drosophila melanogaster]
Length = 236
Score = 269 bits (683), Expect = 3e-71
Identities = 51/217 (23%), Positives = 85/217 (38%), Gaps = 18/217 (8%)
Query: 46 KNSLLVVPFHNIFILVGMFYSGLTQDLETVMWKGFLTSIPIQVIYNYIIYINLLPLKKST 105
K+ L V IL+ M Y ++ F S+ + +++ LL + +
Sbjct: 11 KHQLFHVSLSLATILICMAYMQYRRNW--AHLGSFWDSLVVPIVFL----GELLKVVLAR 64
Query: 106 RNDHQNNSSGSAINNNNNNNNNNVPLLIGSSIFVSIVLSLPLFVVIILMGAPVYKYSLKT 165
+ +A N+ L+G + L F+ IIL GAPV +T
Sbjct: 65 FYGKVEDGVLTAKQRQKKNSYFTPRELLGGFTLQFLCTLLYAFICIIL-GAPVLGNYEQT 123
Query: 166 LYLSLHLSQLIFNPLIILSNLN-------VNKIKRLFKQDHLYRIIFHHGILSSVLLTLG 218
L+L ++ L +P + L K + K + +F + L +L
Sbjct: 124 FVLALLMTLLTVSPTVFLLGGGGALQVCFCEKPDFVTKCEDTALNLFKYNALGGIL---- 179
Query: 219 GCWLGVIPIPLDWDRPWQQWPITLLVGGYLGGVVGGV 255
G W G + PLDW R WQ +PI ++G LG +G +
Sbjct: 180 GAWAGSVVAPLDWGRDWQAYPIPNVIGALLGSALGNI 216
Score = 269 bits (683), Expect = 3e-71
Identities = 51/217 (23%), Positives = 85/217 (38%), Gaps = 18/217 (8%)
Query: 46 KNSLLVVPFHNIFILVGMFYSGLTQDLETVMWKGFLTSIPIQVIYNYIIYINLLPLKKST 105
K+ L V IL+ M Y ++ F S+ + +++ LL + +
Sbjct: 11 KHQLFHVSLSLATILICMAYMQYRRNW--AHLGSFWDSLVVPIVFL----GELLKVVLAR 64
Query: 106 RNDHQNNSSGSAINNNNNNNNNNVPLLIGSSIFVSIVLSLPLFVVIILMGAPVYKYSLKT 165
+ +A N+ L+G + L F+ IIL GAPV +T
Sbjct: 65 FYGKVEDGVLTAKQRQKKNSYFTPRELLGGFTLQFLCTLLYAFICIIL-GAPVLGNYEQT 123
Query: 166 LYLSLHLSQLIFNPLIILSNLN-------VNKIKRLFKQDHLYRIIFHHGILSSVLLTLG 218
L+L ++ L +P + L K + K + +F + L +L
Sbjct: 124 FVLALLMTLLTVSPTVFLLGGGGALQVCFCEKPDFVTKCEDTALNLFKYNALGGIL---- 179
Query: 219 GCWLGVIPIPLDWDRPWQQWPITLLVGGYLGGVVGGV 255
G W G + PLDW R WQ +PI ++G LG +G +
Sbjct: 180 GAWAGSVVAPLDWGRDWQAYPIPNVIGALLGSALGNI 216
>gb|AAF49133.1| (AE003516) CG9376 gene product [Drosophila melanogaster]
Length = 209
Score = 242 bits (613), Expect = 5e-63
Identities = 46/200 (23%), Positives = 78/200 (39%), Gaps = 18/200 (9%)
Query: 63 MFYSGLTQDLETVMWKGFLTSIPIQVIYNYIIYINLLPLKKSTRNDHQNNSSGSAINNNN 122
M Y ++ F S+ + +++ LL + + + +A
Sbjct: 1 MAYMQYRRNW--AHLGSFWDSLVVPIVFL----GELLKVVLARFYGKVEDGVLTAKQRQK 54
Query: 123 NNNNNNVPLLIGSSIFVSIVLSLPLFVVIILMGAPVYKYSLKTLYLSLHLSQLIFNPLII 182
N+ L+G + L F+ IIL GAPV +T L+L ++ L +P +
Sbjct: 55 KNSYFTPRELLGGFTLQFLCTLLYAFICIIL-GAPVLGNYEQTFVLALLMTLLTVSPTVF 113
Query: 183 LSNLN-------VNKIKRLFKQDHLYRIIFHHGILSSVLLTLGGCWLGVIPIPLDWDRPW 235
L K + K + +F + L +L G W G + PLDW R W
Sbjct: 114 LLGGGGALQVCFCEKPDFVTKCEDTALNLFKYNALGGIL----GAWAGSVVAPLDWGRDW 169
Query: 236 QQWPITLLVGGYLGGVVGGV 255
Q +PI ++G LG +G +
Sbjct: 170 QAYPIPNVIGALLGSALGNI 189
>ref|NP_010588.1| (NC_001136) Glycosylphosphatidylinositol (GPI) assembly; Gpi11p
[Saccharomyces cerevisiae]
pir||S61188 probable membrane protein YDR302w - yeast (Saccharomyces
cerevisiae)
gb|AAB64738.1| (U28374) Ydr302wp [Saccharomyces cerevisiae]
Length = 219
Score = 239 bits (604), Expect = 5e-62
Identities = 76/257 (29%), Positives = 115/257 (44%), Gaps = 49/257 (19%)
Query: 1 MPA---AIRPMKKTVSFSKDVS-NNNNNLESDSDTKQSPQSYLTFIPQIKNSLLVVPFHN 56
MPA + +KKTVSFS D + + N E + P Y+ K L+ P+H
Sbjct: 1 MPAKKRTRKTVKKTVSFSDDTTLTTHQNREKKNVDHDRPPVYVR-----KTPLMTFPYHL 55
Query: 57 IFILVGMFYSGLTQDLETVMWKGFLTSIPIQVIYNYIIYINLLPLKKSTRNDHQNNSSGS 116
+ +L +Y ++ + TV FL IP QV Y + Q N
Sbjct: 56 VALL--YYYVFVSSNFNTVKLLSFL--IPTQVAYLVL----------------QFNKCTV 95
Query: 117 AINNNNNNNNNNVPLLIGSSIFVSIVLSLPLFVVIILMGAPVYKYSLKTLYLSLHLSQLI 176
N N + + +G V+ +LS P ++ IL GAP+ +T LSLH + L
Sbjct: 96 YGNKIIKINYSLTIICLG----VTFLLSFPTMLLTILFGAPLMDLLWETWLLSLHFAFLA 151
Query: 177 FNPLIILSNLNVNKIKRLFKQDHLYRIIFHHGILSSVLLTLGGCWLGVIPIPLDWDRPWQ 236
+ + S N + L+K+ ++ ++ G W+ + IPLDWDR WQ
Sbjct: 152 YPA--VYSVFNCDFKVGLWKKYFIFIVV--------------GGWISCVVIPLDWDRDWQ 195
Query: 237 QWPITLLVGGYLGGVVG 253
WPI ++VGGYLG +VG
Sbjct: 196 NWPIPIVVGGYLGALVG 212
>ref|NP_032864.1| (NM_008838) phosphatidylinositol glycan, class F [Mus musculus]
dbj|BAA08818.1| (D50264) phosphatidylinositol glycan class F [Mus musculus]
Length = 219
Score = 185 bits (467), Expect = 5e-46
Identities = 39/125 (31%), Positives = 68/125 (54%), Gaps = 1/125 (0%)
Query: 140 SIVLSLPLFVVIILMGAPVYKYSLKTLYLSLHLSQLIFNPLIILSNLNVNKIKRLFKQDH 199
++ L ++ +L GAP+ + L+T ++ LS P + L N+ R+F ++
Sbjct: 87 FLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLSTFTTVPCLCLLGPNLKAWLRVFSRNG 146
Query: 200 LYRIIFHHGILSSVLLTLGGCWLGVIPIPLDWDRPWQQWPITLLVGGYLGGVVGGVLSLI 259
+ I+ + + + + + G WLG PIPLDW+RPWQ WPI+ +G G V G V+S +
Sbjct: 147 VTS-IWENSLQITTISSFTGAWLGAFPIPLDWERPWQVWPISCTLGATFGYVAGLVISPL 205
Query: 260 VNYFS 264
Y++
Sbjct: 206 WIYWN 210
>ref|NP_002634.1| (NM_002643) phosphatidylinositol glycan, class F [Homo sapiens]
sp|Q07326|PIGF_HUMAN Phosphatidylinositol-glycan biosynthesis, class F protein (PIG-F)
pir||A46097 GPI-anchor biosynthesis protein PIG-F - human
dbj|BAA02697.1| (D13435) PIG-F [Homo sapiens]
Length = 219
Score = 185 bits (467), Expect = 6e-46
Identities = 39/125 (31%), Positives = 69/125 (55%), Gaps = 1/125 (0%)
Query: 140 SIVLSLPLFVVIILMGAPVYKYSLKTLYLSLHLSQLIFNPLIILSNLNVNKIKRLFKQDH 199
++ V+ +L GAP+ + +L+T ++ LS P + L N+ R+F ++
Sbjct: 87 FLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNG 146
Query: 200 LYRIIFHHGILSSVLLTLGGCWLGVIPIPLDWDRPWQQWPITLLVGGYLGGVVGGVLSLI 259
+ I+ + + + + + G WLG +PIPLDW+RPWQ WPI+ +G G V G V+S +
Sbjct: 147 VTS-IWENSLQITTISSFVGAWLGALPIPLDWERPWQVWPISCTLGATFGYVAGLVISPL 205
Query: 260 VNYFS 264
Y++
Sbjct: 206 WIYWN 210
>pir||T40997 probable short-chain dehydrogenase - fission yeast
(Schizosaccharomyces pombe)
emb|CAB40182.1| (AL049559) putative short-chain dehydrogenase [Schizosaccharomyces
pombe]
Length = 503
Score = 158 bits (396), Expect = 1e-37
Identities = 42/136 (30%), Positives = 63/136 (45%), Gaps = 14/136 (10%)
Query: 128 NVPLLIGSSIFVSIVLSLPLFVVIILMGAPVYKYSLKTLYLSLHLSQLIFNPLIILSNLN 187
L+ + ++ SL + +++ GAP+ T + +L LS PL N
Sbjct: 374 TPKRLLAGAA-SMLIGSLLISFILVAFGAPLLHDFHLTYFCALTLSVFTVYPLASTLAFN 432
Query: 188 VNKIKRLFKQDHLYRIIFHHGILSSVLLTLG---GCWLGVIPIPLDWDRPWQQWPITLLV 244
+ +R + I S L + G G W G PIPLDWDRPWQ WPIT+++
Sbjct: 433 TEQWQRFLTLKSF------NVIGSMQLRSWGPIIGAWFGAFPIPLDWDRPWQAWPITIVI 486
Query: 245 GGYLGG----VVGGVL 256
G +LG +VG +L
Sbjct: 487 GAFLGYAFAAIVGEIL 502
>gb|AAF18502.1|AC010924_15 (AC010924) ESTs gb|AI992787, gb|T20398 come from this gene.
[Arabidopsis thaliana]
Length = 270
Score = 144 bits (361), Expect = 1e-33
Identities = 46/200 (23%), Positives = 73/200 (36%), Gaps = 17/200 (8%)
Query: 46 KNSLLVVPFHNIFILVGMFYS----GLTQDLETVMWKGFLTSIPIQVIYNYIIYINLLPL 101
KN + V Y L V K + I + +++I P+
Sbjct: 8 KNPEISVSITISTWGAFAVYVITGLFLIFGFHVVRNKYSVDLISDPTLTLRLLWIIEFPI 67
Query: 102 KKSTRNDHQNNSSGSAINNNNNNNNNNVPLLIGSSIFVSIVLSLPLFVVIILMGAPVYKY 161
+ N + +G S+ I +L + + +GAP+
Sbjct: 68 VVIIYS--------LFRRNPEKCSYFRA---VGRSLVGLIAGALINALGAVSLGAPIGMQ 116
Query: 162 SL-KTLYLSLHLSQLIFNPLIILSNLNVNKIKRLFKQDHLYRIIFHHGILSSVLLTLGGC 220
SL KT++ S +S P + + R+F II H +L + G
Sbjct: 117 SLSKTIHWSFLMSVFTVVPATAVLGASWIDWHRIFASLKPIGII-EHMLLVPAYGAIIGG 175
Query: 221 WLGVIPIPLDWDRPWQQWPI 240
W G P+PLDW+RPWQ+WPI
Sbjct: 176 WFGAWPMPLDWERPWQEWPI 195
>gb|AAH21725.1|AAH21725 (BC021725) Similar to phosphatidylinositol glycan, class F [Homo
sapiens]
Length = 206
Score = 144 bits (360), Expect = 2e-33
Identities = 29/97 (29%), Positives = 53/97 (53%), Gaps = 1/97 (1%)
Query: 140 SIVLSLPLFVVIILMGAPVYKYSLKTLYLSLHLSQLIFNPLIILSNLNVNKIKRLFKQDH 199
++ V+ +L GAP+ + +L+T ++ LS P + L N+ R+F ++
Sbjct: 87 FLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNG 146
Query: 200 LYRIIFHHGILSSVLLTLGGCWLGVIPIPLDWDRPWQ 236
+ I+ + + + + + G WLG +PIPLDW+RPWQ
Sbjct: 147 VTS-IWENSLQITTISSFVGAWLGALPIPLDWERPWQ 182
>pir||T29643 hypothetical protein F49E8.1 - Caenorhabditis elegans
Length = 571
Score = 126 bits (315), Expect = 4e-28
Identities = 29/107 (27%), Positives = 50/107 (46%), Gaps = 4/107 (3%)
Query: 147 LFVVIILMGAPVYKYSLKTLYLSLHLSQLIFNPLIILSNLN---VNKIKRLFKQDHLYRI 203
+++ +L GAP + + T L+ L+ + P + L + V I +LF +
Sbjct: 18 FYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLFSCEG-NPT 76
Query: 204 IFHHGILSSVLLTLGGCWLGVIPIPLDWDRPWQQWPITLLVGGYLGG 250
+L + + G W PLDWDR WQ++P+ LVG ++G
Sbjct: 77 PKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIGA 123
>ref|NP_501226.1| (NM_068825) F49E8.1.p [Caenorhabditis elegans]
gb|AAB03156.2| (U61949) Hypothetical protein F49E8.1 [Caenorhabditis elegans]
Length = 553
Score = 126 bits (314), Expect = 5e-28
Identities = 29/107 (27%), Positives = 50/107 (46%), Gaps = 4/107 (3%)
Query: 147 LFVVIILMGAPVYKYSLKTLYLSLHLSQLIFNPLIILSNLN---VNKIKRLFKQDHLYRI 203
+++ +L GAP + + T L+ L+ + P + L + V I +LF +
Sbjct: 18 FYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLFSCEG-NPT 76
Query: 204 IFHHGILSSVLLTLGGCWLGVIPIPLDWDRPWQQWPITLLVGGYLGG 250
+L + + G W PLDWDR WQ++P+ LVG ++G
Sbjct: 77 PKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIGA 123
>ref|NP_173057.1| (NM_101473) hypothetical protein [Arabidopsis thaliana]
Length = 160
Score = 102 bits (253), Expect = 6e-21
Identities = 22/64 (34%), Positives = 30/64 (46%), Gaps = 1/64 (1%)
Query: 177 FNPLIILSNLNVNKIKRLFKQDHLYRIIFHHGILSSVLLTLGGCWLGVIPIPLDWDRPWQ 236
P + + R+F II H +L + G W G P+PLDW+RPWQ
Sbjct: 23 VVPATAVLGASWIDWHRIFASLKPIGII-EHMLLVPAYGAIIGGWFGAWPMPLDWERPWQ 81
Query: 237 QWPI 240
+WPI
Sbjct: 82 EWPI 85
>ref|XP_018442.1| (XM_018442) similar to phosphatidylinositol glycan, class F (H.
sapiens) [Homo sapiens]
Length = 153
Score = 87.2 bits (214), Expect = 2e-16
Identities = 16/84 (19%), Positives = 35/84 (41%), Gaps = 2/84 (2%)
Query: 120 NNNNNNNNNVPLLIGSSIFVSIVLSLPLFVVIILMGAPVYKYSLKTLYLSLHLSQLIFNP 179
++ + L ++ V+ +L GAP+ + +L+T ++ LS P
Sbjct: 69 KISSLSYKVTRFL--KCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTIGP 126
Query: 180 LIILSNLNVNKIKRLFKQDHLYRI 203
+ L N+ R+F ++ + I
Sbjct: 127 YLCLLGPNLKAWLRVFSRNGVTSI 150
CPU time: 64.97 user secs. 2.55 sys. secs 67.52 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.316 0.162 0.496
Gapped
Lambda K H
0.270 0.0570 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 148202644
Number of Sequences: 887402
Number of extensions: 6152432
Number of successful extensions: 33496
Number of sequences better than 10.0: 131
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 118
Number of HSP's that attempted gapping in prelim test: 33146
Number of HSP's gapped (non-prelim): 273
length of query: 265
length of database: 277,845,442
effective HSP length: 53
effective length of query: 212
effective length of database: 230,813,136
effective search space: 48932384832
effective search space used: 48932384832
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.3 bits)
S2: 73 (32.6 bits)
