Sequences with E-value BETTER than threshold (0.002) 370 e-101
213 2e-54
184 9e-46
183 4e-45
182 5e-45
173 2e-42
172 5e-42
154 1e-36
132 6e-30
131 8e-30
115 1e-24
100 4e-20
53 8e-06
Alignments
>pir||T40997 probable short-chain dehydrogenase - fission yeast
(Schizosaccharomyces pombe)
emb|CAB40182.1| (AL049559) putative short-chain dehydrogenase [Schizosaccharomyces
pombe]
Length = 503
Score = 370 bits (941), Expect = e-101
Identities = 241/241 (100%), Positives = 241/241 (100%)
Query: 1 MKNHCRNSSPHDNPILEYLFALVSLLAWPFYRRKLDSLVYQYALEKGYRQPSSSRNSWIF 60
MKNHCRNSSPHDNPILEYLFALVSLLAWPFYRRKLDSLVYQYALEKGYRQPSSSRNSWIF
Sbjct: 263 MKNHCRNSSPHDNPILEYLFALVSLLAWPFYRRKLDSLVYQYALEKGYRQPSSSRNSWIF 322
Query: 61 TLLLTFTQLTIFYLSLNCLIENPYRMLRNTFPIWFIMQTLQIYIQSPRPPLTPKRLLAGA 120
TLLLTFTQLTIFYLSLNCLIENPYRMLRNTFPIWFIMQTLQIYIQSPRPPLTPKRLLAGA
Sbjct: 323 TLLLTFTQLTIFYLSLNCLIENPYRMLRNTFPIWFIMQTLQIYIQSPRPPLTPKRLLAGA 382
Query: 121 ASMLIGSLLISFILVAFGAPLLHDFHLTYFCALTLSVFTVYPLASTLAFNTEQWQRFLTL 180
ASMLIGSLLISFILVAFGAPLLHDFHLTYFCALTLSVFTVYPLASTLAFNTEQWQRFLTL
Sbjct: 383 ASMLIGSLLISFILVAFGAPLLHDFHLTYFCALTLSVFTVYPLASTLAFNTEQWQRFLTL 442
Query: 181 KSFNVIGSMQLRSWGPIIGAWFGAFPIPLDWDRPWQAWPITIVIGAFLGYAFAAIVGEIL 240
KSFNVIGSMQLRSWGPIIGAWFGAFPIPLDWDRPWQAWPITIVIGAFLGYAFAAIVGEIL
Sbjct: 443 KSFNVIGSMQLRSWGPIIGAWFGAFPIPLDWDRPWQAWPITIVIGAFLGYAFAAIVGEIL 502
Query: 241 Q 241
Q
Sbjct: 503 Q 503
>ref|NP_010588.1| (NC_001136) Glycosylphosphatidylinositol (GPI) assembly; Gpi11p
[Saccharomyces cerevisiae]
pir||S61188 probable membrane protein YDR302w - yeast (Saccharomyces
cerevisiae)
gb|AAB64738.1| (U28374) Ydr302wp [Saccharomyces cerevisiae]
Length = 219
Score = 213 bits (538), Expect = 2e-54
Identities = 50/215 (23%), Positives = 78/215 (36%), Gaps = 44/215 (20%)
Query: 29 PFYRRKLDSLVYQY---ALEKGYRQPSSSRNSWIFTLLLTFTQLTIFYLSLNCLIENPYR 85
P Y RK + + Y AL Y SS+ N+ L TQ+ L N +
Sbjct: 40 PVYVRKTPLMTFPYHLVALLYYYVFVSSNFNTVKLLSFLIPTQVAYLVLQFNKCTVYGNK 99
Query: 86 MLRNTFPIWFIMQTLQIYIQSPRPPLTPKRLLAGAASMLIGSLLISFILVAFGAPLLHDF 145
+++ + + I + S + + FGAPL+
Sbjct: 100 IIKINYSLTII----------------------CLGVTFLLSFPTMLLTILFGAPLMDLL 137
Query: 146 HLTYFCALTLSVFTVYPLASTL---AFNTEQWQRFLTLKSFNVIGSMQLRSWGPIIGAWF 202
T+ +L + F YP ++ F W+++ ++G W
Sbjct: 138 WETWLLSLHFA-FLAYPAVYSVFNCDFKVGLWKKYFIFI---------------VVGGWI 181
Query: 203 GAFPIPLDWDRPWQAWPITIVIGAFLGYAFAAIVG 237
IPLDWDR WQ WPI IV+G +LG +G
Sbjct: 182 SCVVIPLDWDRDWQNWPIPIVVGGYLGALVGYTIG 216
>gb|AAF18502.1|AC010924_15 (AC010924) ESTs gb|AI992787, gb|T20398 come from this gene.
[Arabidopsis thaliana]
Length = 270
Score = 184 bits (465), Expect = 9e-46
Identities = 53/151 (35%), Positives = 81/151 (53%), Gaps = 9/151 (5%)
Query: 79 LIENPYRMLR----NTFPIWFIMQTLQIYIQSPRPPLTPKRLLAGAASMLIGSLLISFIL 134
LI +P LR FPI I+ +L + ++P + + ++ G+L+ +
Sbjct: 49 LISDPTLTLRLLWIIEFPIVVIIYSL--FRRNPEKCSYFRAVGRSLVGLIAGALINALGA 106
Query: 135 VAFGAPL-LHDFHLTYFCALTLSVFTVYPLASTLAFNTEQWQR-FLTLKSFNVIGSMQL- 191
V+ GAP+ + T + +SVFTV P + L + W R F +LK +I M L
Sbjct: 107 VSLGAPIGMQSLSKTIHWSFLMSVFTVVPATAVLGASWIDWHRIFASLKPIGIIEHMLLV 166
Query: 192 RSWGPIIGAWFGAFPIPLDWDRPWQAWPITI 222
++G IIG WFGA+P+PLDW+RPWQ WPI +
Sbjct: 167 PAYGAIIGGWFGAWPMPLDWERPWQEWPICV 197
>ref|NP_002634.1| (NM_002643) phosphatidylinositol glycan, class F [Homo sapiens]
sp|Q07326|PIGF_HUMAN Phosphatidylinositol-glycan biosynthesis, class F protein (PIG-F)
pir||A46097 GPI-anchor biosynthesis protein PIG-F - human
dbj|BAA02697.1| (D13435) PIG-F [Homo sapiens]
Length = 219
Score = 183 bits (460), Expect = 4e-45
Identities = 48/139 (34%), Positives = 66/139 (46%), Gaps = 4/139 (2%)
Query: 105 QSPRPPLTPK--RLLAGAASMLIGSLLISFILVAFGAPLLHDFHLTYFCALTLSVFTVYP 162
S R L+ K L L+ I V +GAPL+ T+ A+ LS FT P
Sbjct: 67 SSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVP 126
Query: 163 LASTLAFNTEQWQRFLTLKSFNVI--GSMQLRSWGPIIGAWFGAFPIPLDWDRPWQAWPI 220
L N + W R + I S+Q+ + +GAW GA PIPLDW+RPWQ WPI
Sbjct: 127 CLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQVWPI 186
Query: 221 TIVIGAFLGYAFAAIVGEI 239
+ +GA GY ++ +
Sbjct: 187 SCTLGATFGYVAGLVISPL 205
>ref|NP_032864.1| (NM_008838) phosphatidylinositol glycan, class F [Mus musculus]
dbj|BAA08818.1| (D50264) phosphatidylinositol glycan class F [Mus musculus]
Length = 219
Score = 182 bits (459), Expect = 5e-45
Identities = 51/140 (36%), Positives = 70/140 (49%), Gaps = 4/140 (2%)
Query: 104 IQSPRPPLTPK--RLLAGAASMLIGSLLISFILVAFGAPLLHDFHLTYFCALTLSVFTVY 161
+ S R L+ K R L L+ L+ I V +GAPL+ T+ A+ LS FT
Sbjct: 66 VSSKRSSLSHKVTRALKCCVCFLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLSTFTTV 125
Query: 162 PLASTLAFNTEQWQRFLTLKSFNVI--GSMQLRSWGPIIGAWFGAFPIPLDWDRPWQAWP 219
P L N + W R + I S+Q+ + GAW GAFPIPLDW+RPWQ WP
Sbjct: 126 PCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFTGAWLGAFPIPLDWERPWQVWP 185
Query: 220 ITIVIGAFLGYAFAAIVGEI 239
I+ +GA GY ++ +
Sbjct: 186 ISCTLGATFGYVAGLVISPL 205
>gb|AAL48412.1| (AY070790) AT13969p [Drosophila melanogaster]
Length = 236
Score = 173 bits (436), Expect = 2e-42
Identities = 47/138 (34%), Positives = 67/138 (48%), Gaps = 6/138 (4%)
Query: 104 IQSPRPPLTPKRLLAGAASMLIGSLLISFILVAFGAPLLHDFHLTYFCALTLSVFTVYPL 163
Q TP+ LL G + +LL +FI + GAP+L ++ T+ AL +++ TV P
Sbjct: 79 RQKKNSYFTPRELLGGFTLQFLCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPT 138
Query: 164 ASTLAFNTE------QWQRFLTLKSFNVIGSMQLRSWGPIIGAWFGAFPIPLDWDRPWQA 217
L + F+T + + + G I+GAW G+ PLDW R WQA
Sbjct: 139 VFLLGGGGALQVCFCEKPDFVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQA 198
Query: 218 WPITIVIGAFLGYAFAAI 235
+PI VIGA LG A I
Sbjct: 199 YPIPNVIGALLGSALGNI 216
>gb|AAF49133.1| (AE003516) CG9376 gene product [Drosophila melanogaster]
Length = 209
Score = 172 bits (433), Expect = 5e-42
Identities = 47/138 (34%), Positives = 67/138 (48%), Gaps = 6/138 (4%)
Query: 104 IQSPRPPLTPKRLLAGAASMLIGSLLISFILVAFGAPLLHDFHLTYFCALTLSVFTVYPL 163
Q TP+ LL G + +LL +FI + GAP+L ++ T+ AL +++ TV P
Sbjct: 52 RQKKNSYFTPRELLGGFTLQFLCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPT 111
Query: 164 ASTLAFNTE------QWQRFLTLKSFNVIGSMQLRSWGPIIGAWFGAFPIPLDWDRPWQA 217
L + F+T + + + G I+GAW G+ PLDW R WQA
Sbjct: 112 VFLLGGGGALQVCFCEKPDFVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQA 171
Query: 218 WPITIVIGAFLGYAFAAI 235
+PI VIGA LG A I
Sbjct: 172 YPIPNVIGALLGSALGNI 189
>gb|AAH21725.1|AAH21725 (BC021725) Similar to phosphatidylinositol glycan, class F [Homo
sapiens]
Length = 206
Score = 154 bits (387), Expect = 1e-36
Identities = 41/116 (35%), Positives = 54/116 (46%), Gaps = 4/116 (3%)
Query: 105 QSPRPPLTPK--RLLAGAASMLIGSLLISFILVAFGAPLLHDFHLTYFCALTLSVFTVYP 162
S R L+ K L L+ I V +GAPL+ T+ A+ LS FT P
Sbjct: 67 SSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVP 126
Query: 163 LASTLAFNTEQWQRFLTLKSFNVI--GSMQLRSWGPIIGAWFGAFPIPLDWDRPWQ 216
L N + W R + I S+Q+ + +GAW GA PIPLDW+RPWQ
Sbjct: 127 CLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQ 182
>pir||T29643 hypothetical protein F49E8.1 - Caenorhabditis elegans
Length = 571
Score = 132 bits (330), Expect = 6e-30
Identities = 37/119 (31%), Positives = 56/119 (46%), Gaps = 10/119 (8%)
Query: 120 AASMLIGSLLISFIL-VAFGAPLLHDFHLTYFCALTLSVFTVYPLASTLAFNTEQWQRFL 178
AA + S + +IL V FGAP D T A L+ + P F++E+ +
Sbjct: 7 AARFVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFL--FDSEERAVEV 64
Query: 179 TLKSFNVIGS-------MQLRSWGPIIGAWFGAFPIPLDWDRPWQAWPITIVIGAFLGY 230
L+ F+ G+ + S +GAW A PLDWDR WQ +P+ ++G F+G
Sbjct: 65 ILQLFSCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIGA 123
>ref|NP_501226.1| (NM_068825) F49E8.1.p [Caenorhabditis elegans]
gb|AAB03156.2| (U61949) Hypothetical protein F49E8.1 [Caenorhabditis elegans]
Length = 553
Score = 131 bits (329), Expect = 8e-30
Identities = 37/119 (31%), Positives = 56/119 (46%), Gaps = 10/119 (8%)
Query: 120 AASMLIGSLLISFIL-VAFGAPLLHDFHLTYFCALTLSVFTVYPLASTLAFNTEQWQRFL 178
AA + S + +IL V FGAP D T A L+ + P F++E+ +
Sbjct: 7 AARFVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFL--FDSEERAVEV 64
Query: 179 TLKSFNVIGS-------MQLRSWGPIIGAWFGAFPIPLDWDRPWQAWPITIVIGAFLGY 230
L+ F+ G+ + S +GAW A PLDWDR WQ +P+ ++G F+G
Sbjct: 65 ILQLFSCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIGA 123
>ref|NP_173057.1| (NM_101473) hypothetical protein [Arabidopsis thaliana]
Length = 160
Score = 115 bits (286), Expect = 1e-24
Identities = 32/77 (41%), Positives = 43/77 (55%), Gaps = 2/77 (2%)
Query: 148 TYFCALTLSVFTVYPLASTLAFNTEQWQR-FLTLKSFNVIGSMQL-RSWGPIIGAWFGAF 205
T + V P + L + W R F +LK +I M L ++G IIG WFGA+
Sbjct: 11 TSAWRFVHDLKDVVPATAVLGASWIDWHRIFASLKPIGIIEHMLLVPAYGAIIGGWFGAW 70
Query: 206 PIPLDWDRPWQAWPITI 222
P+PLDW+RPWQ WPI +
Sbjct: 71 PMPLDWERPWQEWPICV 87
>ref|XP_018442.1| (XM_018442) similar to phosphatidylinositol glycan, class F (H.
sapiens) [Homo sapiens]
Length = 153
Score = 99.7 bits (246), Expect = 4e-20
Identities = 24/84 (28%), Positives = 33/84 (38%), Gaps = 2/84 (2%)
Query: 105 QSPRPPLTPK--RLLAGAASMLIGSLLISFILVAFGAPLLHDFHLTYFCALTLSVFTVYP 162
S L+ K R L L+ I V +GAPL+ T+ A+ LS FT+ P
Sbjct: 67 SSKISSLSYKVTRFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTIGP 126
Query: 163 LASTLAFNTEQWQRFLTLKSFNVI 186
L N + W R + I
Sbjct: 127 YLCLLGPNLKAWLRVFSRNGVTSI 150
>ref|NP_173056.1| (NM_101472) unknown protein [Arabidopsis thaliana]
Length = 98
Score = 52.5 bits (125), Expect = 8e-06
Identities = 12/52 (23%), Positives = 22/52 (42%), Gaps = 6/52 (11%)
Query: 79 LIENPYRMLR----NTFPIWFIMQTLQIYIQSPRPPLTPKRLLAGAASMLIG 126
LI +P LR FPI I+ +L + ++P + + ++ G
Sbjct: 49 LISDPTLTLRLLWIIEFPIVVIIYSL--FRRNPEKCSYFRAVGRSLVGLIAG 98
CPU time: 66.86 user secs. 2.17 sys. secs 69.03 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.318 0.167 0.518
Gapped
Lambda K H
0.270 0.0586 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 149096764
Number of Sequences: 887402
Number of extensions: 6922853
Number of successful extensions: 33752
Number of sequences better than 10.0: 141
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 126
Number of HSP's that attempted gapping in prelim test: 33662
Number of HSP's gapped (non-prelim): 175
length of query: 241
length of database: 277,845,442
effective HSP length: 48
effective length of query: 193
effective length of database: 235,250,146
effective search space: 45403278178
effective search space used: 45403278178
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.9 bits)
S2: 72 (32.1 bits)
