Sequences with E-value BETTER than threshold (0.002) 192 3e-48
191 4e-48
158 4e-38
157 7e-38
155 3e-37
144 9e-34
138 4e-32
129 2e-29
127 8e-29
109 2e-23
101 4e-21
86 2e-16
Alignments
>pir||T29643 hypothetical protein F49E8.1 - Caenorhabditis elegans
Length = 571
Score = 192 bits (484), Expect = 3e-48
Identities = 130/130 (100%), Positives = 130/130 (100%)
Query: 1 MLSLPVAARFVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEER 60
MLSLPVAARFVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEER
Sbjct: 1 MLSLPVAARFVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEER 60
Query: 61 AVEVILQLFSCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCF 120
AVEVILQLFSCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCF
Sbjct: 61 AVEVILQLFSCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCF 120
Query: 121 IGARQKTLAT 130
IGARQKTLAT
Sbjct: 121 IGARQKTLAT 130
>ref|NP_501226.1| (NM_068825) F49E8.1.p [Caenorhabditis elegans]
gb|AAB03156.2| (U61949) Hypothetical protein F49E8.1 [Caenorhabditis elegans]
Length = 553
Score = 191 bits (482), Expect = 4e-48
Identities = 130/130 (100%), Positives = 130/130 (100%)
Query: 1 MLSLPVAARFVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEER 60
MLSLPVAARFVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEER
Sbjct: 1 MLSLPVAARFVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEER 60
Query: 61 AVEVILQLFSCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCF 120
AVEVILQLFSCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCF
Sbjct: 61 AVEVILQLFSCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCF 120
Query: 121 IGARQKTLAT 130
IGARQKTLAT
Sbjct: 121 IGARQKTLAT 130
>ref|NP_002634.1| (NM_002643) phosphatidylinositol glycan, class F [Homo sapiens]
sp|Q07326|PIGF_HUMAN Phosphatidylinositol-glycan biosynthesis, class F protein (PIG-F)
pir||A46097 GPI-anchor biosynthesis protein PIG-F - human
dbj|BAA02697.1| (D13435) PIG-F [Homo sapiens]
Length = 219
Score = 158 bits (397), Expect = 4e-38
Identities = 34/113 (30%), Positives = 59/113 (52%), Gaps = 3/113 (2%)
Query: 10 FVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLF 69
+ +S F+++ VL+GAP + T + A L+ + +P + L +A L++F
Sbjct: 86 YFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKA---WLRVF 142
Query: 70 SCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIG 122
S G + E+ L ++ +F+GAW A PLDW+R WQ +P+ +G G
Sbjct: 143 SRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQVWPISCTLGATFG 195
>pir||T40997 probable short-chain dehydrogenase - fission yeast
(Schizosaccharomyces pombe)
emb|CAB40182.1| (AL049559) putative short-chain dehydrogenase [Schizosaccharomyces
pombe]
Length = 503
Score = 157 bits (395), Expect = 7e-38
Identities = 37/118 (31%), Positives = 56/118 (47%), Gaps = 10/118 (8%)
Query: 7 AARFVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALP--AVFLFDSEERAVEV 64
AA + S + +IL V FGAP D T A L+ + P + F++E+ +
Sbjct: 382 AASMLIGSLLISFIL-VAFGAPLLHDFHLTYFCALTLSVFTVYPLASTLAFNTEQWQRFL 440
Query: 65 ILQLFSCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIG 122
L+ F+ G + S +GAW A PLDWDR WQ +P+ ++G F+G
Sbjct: 441 TLKSFNVIG-------SMQLRSWGPIIGAWFGAFPIPLDWDRPWQAWPITIVIGAFLG 491
>ref|NP_032864.1| (NM_008838) phosphatidylinositol glycan, class F [Mus musculus]
dbj|BAA08818.1| (D50264) phosphatidylinositol glycan class F [Mus musculus]
Length = 219
Score = 155 bits (389), Expect = 3e-37
Identities = 34/112 (30%), Positives = 58/112 (51%), Gaps = 3/112 (2%)
Query: 11 VTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLFS 70
+S + +I+ VL+GAP ++ T + A L+ + +P + L +A L++FS
Sbjct: 87 FLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLSTFTTVPCLCLLGPNLKA---WLRVFS 143
Query: 71 CEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIG 122
G + E+ L ++ +F GAW A PLDW+R WQ +P+ +G G
Sbjct: 144 RNGVTSIWENSLQITTISSFTGAWLGAFPIPLDWERPWQVWPISCTLGATFG 195
>gb|AAH21725.1|AAH21725 (BC021725) Similar to phosphatidylinositol glycan, class F [Homo
sapiens]
Length = 206
Score = 144 bits (360), Expect = 9e-34
Identities = 31/100 (31%), Positives = 53/100 (53%), Gaps = 3/100 (3%)
Query: 10 FVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLF 69
+ +S F+++ VL+GAP + T + A L+ + +P + L +A L++F
Sbjct: 86 YFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKA---WLRVF 142
Query: 70 SCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQ 109
S G + E+ L ++ +F+GAW A PLDW+R WQ
Sbjct: 143 SRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQ 182
>gb|AAF18502.1|AC010924_15 (AC010924) ESTs gb|AI992787, gb|T20398 come from this gene.
[Arabidopsis thaliana]
Length = 270
Score = 138 bits (345), Expect = 4e-32
Identities = 24/104 (23%), Positives = 45/104 (43%), Gaps = 4/104 (3%)
Query: 11 VTLSWAVFYILAVLFGAPF-FSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLF 69
+ + + AV GAP + T + ++ + +PA + + + ++F
Sbjct: 95 LIAGALINALGAVSLGAPIGMQSLSKTIHWSFLMSVFTVVPATAVLGA---SWIDWHRIF 151
Query: 70 SCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPL 113
+ E +LL + A +G W A PLDW+R WQ +P+
Sbjct: 152 ASLKPIGIIEHMLLVPAYGAIIGGWFGAWPMPLDWERPWQEWPI 195
>gb|AAL48412.1| (AY070790) AT13969p [Drosophila melanogaster]
Length = 236
Score = 129 bits (322), Expect = 2e-29
Identities = 38/114 (33%), Positives = 58/114 (50%), Gaps = 11/114 (9%)
Query: 16 AVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLFSCEGNP 75
++ + ++ GAP + T VLA +T ++ P VFL LQ+ CE
Sbjct: 103 LLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGG-----ALQVCFCEKPD 157
Query: 76 --TPKESVLL----FNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIGA 123
T E L +N++ LGAWA + V PLDW R WQ YP+P+++G +G+
Sbjct: 158 FVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGALLGS 211
>gb|AAF49133.1| (AE003516) CG9376 gene product [Drosophila melanogaster]
Length = 209
Score = 127 bits (318), Expect = 8e-29
Identities = 38/114 (33%), Positives = 58/114 (50%), Gaps = 11/114 (9%)
Query: 16 AVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLFSCEGNP 75
++ + ++ GAP + T VLA +T ++ P VFL LQ+ CE
Sbjct: 76 LLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGG-----ALQVCFCEKPD 130
Query: 76 --TPKESVLL----FNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIGA 123
T E L +N++ LGAWA + V PLDW R WQ YP+P+++G +G+
Sbjct: 131 FVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGALLGS 184
>ref|NP_010588.1| (NC_001136) Glycosylphosphatidylinositol (GPI) assembly; Gpi11p
[Saccharomyces cerevisiae]
pir||S61188 probable membrane protein YDR302w - yeast (Saccharomyces
cerevisiae)
gb|AAB64738.1| (U28374) Ydr302wp [Saccharomyces cerevisiae]
Length = 219
Score = 109 bits (271), Expect = 2e-23
Identities = 38/115 (33%), Positives = 55/115 (47%), Gaps = 23/115 (20%)
Query: 11 VTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLFS 70
LS +L +LFGAP D++ L + A A PAV+ +F+
Sbjct: 116 FLLS-FPTMLLTILFGAPLM-DLLWETWLLSLHFAFLAYPAVY-------------SVFN 160
Query: 71 CEGNPTPKESVLLFNSVFAFL--GAWAAAAVHPLDWDRWWQRYPLPSLVGCFIGA 123
C+ V L+ F F+ G W + V PLDWDR WQ +P+P +VG ++GA
Sbjct: 161 CDFK------VGLWKKYFIFIVVGGWISCVVIPLDWDRDWQNWPIPIVVGGYLGA 209
>ref|NP_173057.1| (NM_101473) hypothetical protein [Arabidopsis thaliana]
Length = 160
Score = 101 bits (252), Expect = 4e-21
Identities = 19/78 (24%), Positives = 33/78 (41%), Gaps = 3/78 (3%)
Query: 36 TAVLATALTAVSALPAVFLFDSEERAVEVILQLFSCEGNPTPKESVLLFNSVFAFLGAWA 95
T+ +PA + + + ++F+ E +LL + A +G W
Sbjct: 11 TSAWRFVHDLKDVVPATAVLGA---SWIDWHRIFASLKPIGIIEHMLLVPAYGAIIGGWF 67
Query: 96 AAAVHPLDWDRWWQRYPL 113
A PLDW+R WQ +P+
Sbjct: 68 GAWPMPLDWERPWQEWPI 85
>ref|XP_018442.1| (XM_018442) similar to phosphatidylinositol glycan, class F (H.
sapiens) [Homo sapiens]
Length = 153
Score = 86.4 bits (212), Expect = 2e-16
Identities = 17/69 (24%), Positives = 32/69 (45%), Gaps = 3/69 (4%)
Query: 10 FVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLF 69
+ +S F+++ VL+GAP + T + A L+ + P + L +A L++F
Sbjct: 86 YFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTIGPYLCLLGPNLKA---WLRVF 142
Query: 70 SCEGNPTPK 78
S G +
Sbjct: 143 SRNGVTSIW 151
CPU time: 42.84 user secs. 1.99 sys. secs 44.83 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.317 0.171 0.538
Gapped
Lambda K H
0.270 0.0598 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 87432961
Number of Sequences: 887402
Number of extensions: 3988161
Number of successful extensions: 19089
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 26
Number of HSP's that attempted gapping in prelim test: 19044
Number of HSP's gapped (non-prelim): 55
length of query: 130
length of database: 277,845,442
effective HSP length: 49
effective length of query: 81
effective length of database: 234,362,744
effective search space: 18983382264
effective search space used: 18983382264
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.9 bits)
S2: 69 (30.9 bits)
