Sequences with E-value BETTER than threshold (0.002) 342 3e-93
329 2e-89
292 5e-78
221 7e-57
166 3e-40
162 5e-39
151 2e-35
150 2e-35
144 1e-33
144 2e-33
133 2e-30
105 5e-22
Alignments
>ref|NP_002634.1| (NM_002643) phosphatidylinositol glycan, class F [Homo sapiens]
sp|Q07326|PIGF_HUMAN Phosphatidylinositol-glycan biosynthesis, class F protein (PIG-F)
pir||A46097 GPI-anchor biosynthesis protein PIG-F - human
dbj|BAA02697.1| (D13435) PIG-F [Homo sapiens]
Length = 219
Score = 342 bits (870), Expect = 3e-93
Identities = 219/219 (100%), Positives = 219/219 (100%)
Query: 1 MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYL 60
MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYL
Sbjct: 1 MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYL 60
Query: 61 VVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILS 120
VVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILS
Sbjct: 61 VVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILS 120
Query: 121 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERP 180
TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERP
Sbjct: 121 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERP 180
Query: 181 WQVWPISCTLGATFGYVAGLVISPLWIYWNRKQLTYKNN 219
WQVWPISCTLGATFGYVAGLVISPLWIYWNRKQLTYKNN
Sbjct: 181 WQVWPISCTLGATFGYVAGLVISPLWIYWNRKQLTYKNN 219
>ref|NP_032864.1| (NM_008838) phosphatidylinositol glycan, class F [Mus musculus]
dbj|BAA08818.1| (D50264) phosphatidylinositol glycan class F [Mus musculus]
Length = 219
Score = 329 bits (837), Expect = 2e-89
Identities = 192/219 (87%), Positives = 202/219 (91%)
Query: 1 MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYL 60
MKD DIKRLLYT+LLC+FSI LS+FIPS F++NFS+LE HLTWLCICS VT VNL+ YL
Sbjct: 1 MKDTDIKRLLYTNLLCVFSIFLSIFIPSFFVDNFSVLEAHLTWLCICSASVTTVNLLSYL 60
Query: 61 VVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILS 120
VVKPN SSKRSSLSHKVT LKCC+ FLMSCF H+IFVLYGAPLIEL LETFLFAV+LS
Sbjct: 61 VVKPNVSSKRSSLSHKVTRALKCCVCFLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLS 120
Query: 121 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERP 180
TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSF GAWLGA PIPLDWERP
Sbjct: 121 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFTGAWLGAFPIPLDWERP 180
Query: 181 WQVWPISCTLGATFGYVAGLVISPLWIYWNRKQLTYKNN 219
WQVWPISCTLGATFGYVAGLVISPLWIYWNRKQLTYKNN
Sbjct: 181 WQVWPISCTLGATFGYVAGLVISPLWIYWNRKQLTYKNN 219
>gb|AAH21725.1|AAH21725 (BC021725) Similar to phosphatidylinositol glycan, class F [Homo
sapiens]
Length = 206
Score = 292 bits (740), Expect = 5e-78
Identities = 182/183 (99%), Positives = 183/183 (99%)
Query: 1 MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYL 60
MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYL
Sbjct: 1 MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYL 60
Query: 61 VVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILS 120
VVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILS
Sbjct: 61 VVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILS 120
Query: 121 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERP 180
TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERP
Sbjct: 121 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERP 180
Query: 181 WQV 183
WQ+
Sbjct: 181 WQM 183
>ref|XP_018442.1| (XM_018442) similar to phosphatidylinositol glycan, class F (H.
sapiens) [Homo sapiens]
Length = 153
Score = 221 bits (560), Expect = 7e-57
Identities = 141/151 (93%), Positives = 144/151 (94%)
Query: 1 MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYL 60
MKD DIKRLLYTHLLCIFSIILSVFIPSLFLEN SILETHLTWLC+CSGFVTAV+LVLYL
Sbjct: 1 MKDTDIKRLLYTHLLCIFSIILSVFIPSLFLENSSILETHLTWLCVCSGFVTAVSLVLYL 60
Query: 61 VVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILS 120
VVKPNTSSK SSLS+KVT FLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILS
Sbjct: 61 VVKPNTSSKISSLSYKVTRFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILS 120
Query: 121 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIW 151
TFT P LCLLGPNLKAWLRVFSRNGVTSIW
Sbjct: 121 TFTIGPYLCLLGPNLKAWLRVFSRNGVTSIW 151
>pir||T40997 probable short-chain dehydrogenase - fission yeast
(Schizosaccharomyces pombe)
emb|CAB40182.1| (AL049559) putative short-chain dehydrogenase [Schizosaccharomyces
pombe]
Length = 503
Score = 166 bits (418), Expect = 3e-40
Identities = 44/127 (34%), Positives = 61/127 (47%), Gaps = 2/127 (1%)
Query: 79 GFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAW 138
L L+ I V +GAPL+ T+ A+ LS FT P L N + W
Sbjct: 377 RLLAGAASMLIGSLLISFILVAFGAPLLHDFHLTYFCALTLSVFTVYPLASTLAFNTEQW 436
Query: 139 LRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQVWPISCTLGATFGYVA 198
R + I S+Q+ + +GAW GA PIPLDW+RPWQ WPI+ +GA GY
Sbjct: 437 QRFLTLKSFNVI--GSMQLRSWGPIIGAWFGAFPIPLDWDRPWQAWPITIVIGAFLGYAF 494
Query: 199 GLVISPL 205
++ +
Sbjct: 495 AAIVGEI 501
>ref|NP_010588.1| (NC_001136) Glycosylphosphatidylinositol (GPI) assembly; Gpi11p
[Saccharomyces cerevisiae]
pir||S61188 probable membrane protein YDR302w - yeast (Saccharomyces
cerevisiae)
gb|AAB64738.1| (U28374) Ydr302wp [Saccharomyces cerevisiae]
Length = 219
Score = 162 bits (407), Expect = 5e-39
Identities = 44/150 (29%), Positives = 71/150 (47%), Gaps = 19/150 (12%)
Query: 57 VLYLVVKPNTSSKRSSLSHKVTGFLKCC---IYFLMSCFSFHVIFVLYGAPLIELALETF 113
V YLV++ N + + K+ L + FL+S F ++ +L+GAPL++L ET+
Sbjct: 83 VAYLVLQFNKCTVYGNKIIKINYSLTIICLGVTFLLS-FPTMLLTILFGAPLMDLLWETW 141
Query: 114 LFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPI 173
L ++ + F P + VF+ + +W+ I VG W+ + I
Sbjct: 142 LLSLHFA-FLAYPAV----------YSVFNCDFKVGLWKKYF----IFIVVGGWISCVVI 186
Query: 174 PLDWERPWQVWPISCTLGATFGYVAGLVIS 203
PLDW+R WQ WPI +G G + G I
Sbjct: 187 PLDWDRDWQNWPIPIVVGGYLGALVGYTIG 216
>pir||T29643 hypothetical protein F49E8.1 - Caenorhabditis elegans
Length = 571
Score = 151 bits (378), Expect = 2e-35
Identities = 34/114 (29%), Positives = 59/114 (50%), Gaps = 3/114 (2%)
Query: 86 YFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKA---WLRVF 142
+ +S F+++ VL+GAP + T + A L+ + +P + L +A L++F
Sbjct: 10 FVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLF 69
Query: 143 SRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQVWPISCTLGATFGY 196
S G + E+ L ++ +F+GAW A PLDW+R WQ +P+ +G G
Sbjct: 70 SCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIGA 123
>ref|NP_501226.1| (NM_068825) F49E8.1.p [Caenorhabditis elegans]
gb|AAB03156.2| (U61949) Hypothetical protein F49E8.1 [Caenorhabditis elegans]
Length = 553
Score = 150 bits (376), Expect = 2e-35
Identities = 34/114 (29%), Positives = 59/114 (50%), Gaps = 3/114 (2%)
Query: 86 YFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKA---WLRVF 142
+ +S F+++ VL+GAP + T + A L+ + +P + L +A L++F
Sbjct: 10 FVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLF 69
Query: 143 SRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQVWPISCTLGATFGY 196
S G + E+ L ++ +F+GAW A PLDW+R WQ +P+ +G G
Sbjct: 70 SCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIGA 123
>gb|AAL48412.1| (AY070790) AT13969p [Drosophila melanogaster]
Length = 236
Score = 144 bits (362), Expect = 1e-33
Identities = 31/115 (26%), Positives = 54/115 (46%), Gaps = 4/115 (3%)
Query: 89 MSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNGVT 148
+ + I ++ GAP++ +TF+ A++++ T P + LLG + +
Sbjct: 100 LCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGALQVCFCEKPDFV 159
Query: 149 SIWE----NSLQITTISSFVGAWLGALPIPLDWERPWQVWPISCTLGATFGYVAG 199
+ E N + + +GAW G++ PLDW R WQ +PI +GA G G
Sbjct: 160 TKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGALLGSALG 214
>gb|AAF49133.1| (AE003516) CG9376 gene product [Drosophila melanogaster]
Length = 209
Score = 144 bits (360), Expect = 2e-33
Identities = 31/115 (26%), Positives = 54/115 (46%), Gaps = 4/115 (3%)
Query: 89 MSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNGVT 148
+ + I ++ GAP++ +TF+ A++++ T P + LLG + +
Sbjct: 73 LCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGALQVCFCEKPDFV 132
Query: 149 SIWE----NSLQITTISSFVGAWLGALPIPLDWERPWQVWPISCTLGATFGYVAG 199
+ E N + + +GAW G++ PLDW R WQ +PI +GA G G
Sbjct: 133 TKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGALLGSALG 187
>gb|AAF18502.1|AC010924_15 (AC010924) ESTs gb|AI992787, gb|T20398 come from this gene.
[Arabidopsis thaliana]
Length = 270
Score = 133 bits (334), Expect = 2e-30
Identities = 36/90 (40%), Positives = 52/90 (57%), Gaps = 1/90 (1%)
Query: 98 FVLYGAPLIELAL-ETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQ 156
V GAP+ +L +T ++ ++S FT VP +LG + W R+F+ I E+ L
Sbjct: 106 AVSLGAPIGMQSLSKTIHWSFLMSVFTVVPATAVLGASWIDWHRIFASLKPIGIIEHMLL 165
Query: 157 ITTISSFVGAWLGALPIPLDWERPWQVWPI 186
+ + +G W GA P+PLDWERPWQ WPI
Sbjct: 166 VPAYGAIIGGWFGAWPMPLDWERPWQEWPI 195
>ref|NP_173057.1| (NM_101473) hypothetical protein [Arabidopsis thaliana]
Length = 160
Score = 105 bits (262), Expect = 5e-22
Identities = 27/63 (42%), Positives = 36/63 (56%)
Query: 124 TVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQV 183
VP +LG + W R+F+ I E+ L + + +G W GA P+PLDWERPWQ
Sbjct: 23 VVPATAVLGASWIDWHRIFASLKPIGIIEHMLLVPAYGAIIGGWFGAWPMPLDWERPWQE 82
Query: 184 WPI 186
WPI
Sbjct: 83 WPI 85
CPU time: 78.30 user secs. 3.81 sys. secs 82.11 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.321 0.169 0.567
Gapped
Lambda K H
0.270 0.0592 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 158733874
Number of Sequences: 887402
Number of extensions: 8041012
Number of successful extensions: 37633
Number of sequences better than 10.0: 150
Number of HSP's better than 10.0 without gapping: 29
Number of HSP's successfully gapped in prelim test: 122
Number of HSP's that attempted gapping in prelim test: 37533
Number of HSP's gapped (non-prelim): 193
length of query: 219
length of database: 277,845,442
effective HSP length: 47
effective length of query: 172
effective length of database: 236,137,548
effective search space: 40615658256
effective search space used: 40615658256
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.1 bits)
S2: 72 (32.1 bits)
