Sequences with E-value BETTER than threshold (0.002) 334 1e-90
328 4e-89
283 2e-75
213 2e-54
198 1e-49
176 3e-43
149 6e-35
147 1e-34
146 3e-34
145 5e-34
133 3e-30
106 4e-22
Alignments
>ref|NP_002634.1| (NM_002643) phosphatidylinositol glycan, class F [Homo sapiens]
sp|Q07326|PIGF_HUMAN Phosphatidylinositol-glycan biosynthesis, class F protein (PIG-F)
pir||A46097 GPI-anchor biosynthesis protein PIG-F - human
dbj|BAA02697.1| (D13435) PIG-F [Homo sapiens]
Length = 219
Score = 334 bits (848), Expect = 1e-90
Identities = 192/219 (87%), Positives = 202/219 (91%)
Query: 1 MKDTDIKRLLYTNLLCVFSIFLSIFIPSFFVDNFSVLEAHLTWLCICSASVTTVNLLSYL 60
MKD DIKRLLYT+LLC+FSI LS+FIPS F++NFS+LE HLTWLCICS VT VNL+ YL
Sbjct: 1 MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYL 60
Query: 61 VVKPNVSSKRSSLSHKVTRALKCCVCFLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLS 120
VVKPN SSKRSSLSHKVT LKCC+ FLMSCF H+IFVLYGAPLIEL LETFLFAV+LS
Sbjct: 61 VVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILS 120
Query: 121 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFTGAWLGAFPIPLDWERP 180
TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSF GAWLGA PIPLDWERP
Sbjct: 121 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERP 180
Query: 181 WQVWPISCTLGATFGYVAGLVISPLWIYWNRKQLTYKNN 219
WQVWPISCTLGATFGYVAGLVISPLWIYWNRKQLTYKNN
Sbjct: 181 WQVWPISCTLGATFGYVAGLVISPLWIYWNRKQLTYKNN 219
>ref|NP_032864.1| (NM_008838) phosphatidylinositol glycan, class F [Mus musculus]
dbj|BAA08818.1| (D50264) phosphatidylinositol glycan class F [Mus musculus]
Length = 219
Score = 328 bits (834), Expect = 4e-89
Identities = 219/219 (100%), Positives = 219/219 (100%)
Query: 1 MKDTDIKRLLYTNLLCVFSIFLSIFIPSFFVDNFSVLEAHLTWLCICSASVTTVNLLSYL 60
MKDTDIKRLLYTNLLCVFSIFLSIFIPSFFVDNFSVLEAHLTWLCICSASVTTVNLLSYL
Sbjct: 1 MKDTDIKRLLYTNLLCVFSIFLSIFIPSFFVDNFSVLEAHLTWLCICSASVTTVNLLSYL 60
Query: 61 VVKPNVSSKRSSLSHKVTRALKCCVCFLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLS 120
VVKPNVSSKRSSLSHKVTRALKCCVCFLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLS
Sbjct: 61 VVKPNVSSKRSSLSHKVTRALKCCVCFLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLS 120
Query: 121 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFTGAWLGAFPIPLDWERP 180
TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFTGAWLGAFPIPLDWERP
Sbjct: 121 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFTGAWLGAFPIPLDWERP 180
Query: 181 WQVWPISCTLGATFGYVAGLVISPLWIYWNRKQLTYKNN 219
WQVWPISCTLGATFGYVAGLVISPLWIYWNRKQLTYKNN
Sbjct: 181 WQVWPISCTLGATFGYVAGLVISPLWIYWNRKQLTYKNN 219
>gb|AAH21725.1|AAH21725 (BC021725) Similar to phosphatidylinositol glycan, class F [Homo
sapiens]
Length = 206
Score = 283 bits (718), Expect = 2e-75
Identities = 155/183 (84%), Positives = 166/183 (90%)
Query: 1 MKDTDIKRLLYTNLLCVFSIFLSIFIPSFFVDNFSVLEAHLTWLCICSASVTTVNLLSYL 60
MKD DIKRLLYT+LLC+FSI LS+FIPS F++NFS+LE HLTWLCICS VT VNL+ YL
Sbjct: 1 MKDNDIKRLLYTHLLCIFSIILSVFIPSLFLENFSILETHLTWLCICSGFVTAVNLVLYL 60
Query: 61 VVKPNVSSKRSSLSHKVTRALKCCVCFLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLS 120
VVKPN SSKRSSLSHKVT LKCC+ FLMSCF H+IFVLYGAPLIEL LETFLFAV+LS
Sbjct: 61 VVKPNTSSKRSSLSHKVTGFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILS 120
Query: 121 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFTGAWLGAFPIPLDWERP 180
TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSF GAWLGA PIPLDWERP
Sbjct: 121 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERP 180
Query: 181 WQV 183
WQ+
Sbjct: 181 WQM 183
>ref|XP_018442.1| (XM_018442) similar to phosphatidylinositol glycan, class F (H.
sapiens) [Homo sapiens]
Length = 153
Score = 213 bits (539), Expect = 2e-54
Identities = 120/151 (79%), Positives = 133/151 (87%)
Query: 1 MKDTDIKRLLYTNLLCVFSIFLSIFIPSFFVDNFSVLEAHLTWLCICSASVTTVNLLSYL 60
MKDTDIKRLLYT+LLC+FSI LS+FIPS F++N S+LE HLTWLC+CS VT V+L+ YL
Sbjct: 1 MKDTDIKRLLYTHLLCIFSIILSVFIPSLFLENSSILETHLTWLCVCSGFVTAVSLVLYL 60
Query: 61 VVKPNVSSKRSSLSHKVTRALKCCVCFLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLS 120
VVKPN SSK SSLS+KVTR LKCC+ FLMSCF H+IFVLYGAPLIEL LETFLFAV+LS
Sbjct: 61 VVKPNTSSKISSLSYKVTRFLKCCIYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILS 120
Query: 121 TFTTVPCLCLLGPNLKAWLRVFSRNGVTSIW 151
TFT P LCLLGPNLKAWLRVFSRNGVTSIW
Sbjct: 121 TFTIGPYLCLLGPNLKAWLRVFSRNGVTSIW 151
>pir||T40997 probable short-chain dehydrogenase - fission yeast
(Schizosaccharomyces pombe)
emb|CAB40182.1| (AL049559) putative short-chain dehydrogenase [Schizosaccharomyces
pombe]
Length = 503
Score = 198 bits (499), Expect = 1e-49
Identities = 51/144 (35%), Positives = 72/144 (49%), Gaps = 4/144 (2%)
Query: 62 VKPNVSSKRSSLSHKVTRALKCCVCFLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLST 121
++ + S R L+ K R L L+ L+ I V +GAPL+ T+ A+ LS
Sbjct: 362 LQIYIQSPRPPLTPK--RLLAGAASMLIGSLLISFILVAFGAPLLHDFHLTYFCALTLSV 419
Query: 122 FTTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFTGAWLGAFPIPLDWERPW 181
FT P L N + W R + I S+Q+ + GAW GAFPIPLDW+RPW
Sbjct: 420 FTVYPLASTLAFNTEQWQRFLTLKSFNVI--GSMQLRSWGPIIGAWFGAFPIPLDWDRPW 477
Query: 182 QVWPISCTLGATFGYVAGLVISPL 205
Q WPI+ +GA GY ++ +
Sbjct: 478 QAWPITIVIGAFLGYAFAAIVGEI 501
>ref|NP_010588.1| (NC_001136) Glycosylphosphatidylinositol (GPI) assembly; Gpi11p
[Saccharomyces cerevisiae]
pir||S61188 probable membrane protein YDR302w - yeast (Saccharomyces
cerevisiae)
gb|AAB64738.1| (U28374) Ydr302wp [Saccharomyces cerevisiae]
Length = 219
Score = 176 bits (443), Expect = 3e-43
Identities = 51/178 (28%), Positives = 82/178 (45%), Gaps = 27/178 (15%)
Query: 37 LEAHLTWLCICSASVTTVNLLS--------YLVVKPNVSSKRSSLSHKVTRALKCC---V 85
L A L + S++ TV LLS YLV++ N + + K+ +L V
Sbjct: 55 LVALLYYYVFVSSNFNTVKLLSFLIPTQVAYLVLQFNKCTVYGNKIIKINYSLTIICLGV 114
Query: 86 CFLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLSTFTTVPCLCLLGPNLKAWLRVFSRN 145
FL+S F ++ +L+GAPL++L+ ET+L ++ + F P + VF+ +
Sbjct: 115 TFLLS-FPTMLLTILFGAPLMDLLWETWLLSLHFA-FLAYPAV----------YSVFNCD 162
Query: 146 GVTSIWENSLQITTISSFTGAWLGAFPIPLDWERPWQVWPISCTLGATFGYVAGLVIS 203
+W+ + G W+ IPLDW+R WQ WPI +G G + G I
Sbjct: 163 FKVGLWKKYFIFIVV----GGWISCVVIPLDWDRDWQNWPIPIVVGGYLGALVGYTIG 216
>gb|AAL48412.1| (AY070790) AT13969p [Drosophila melanogaster]
Length = 236
Score = 149 bits (373), Expect = 6e-35
Identities = 36/126 (28%), Positives = 56/126 (43%), Gaps = 5/126 (3%)
Query: 79 RALKCCVCFLMSCFLLH-IIFVLYGAPLIELVLETFLFAVVLSTFTTVPCLCLLGPNLKA 137
R L C LL+ I ++ GAP++ +TF+ A++++ T P + LLG
Sbjct: 89 RELLGGFTLQFLCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGAL 148
Query: 138 WLRVFSRNGVTSIWE----NSLQITTISSFTGAWLGAFPIPLDWERPWQVWPISCTLGAT 193
+ + + E N + + GAW G+ PLDW R WQ +PI +GA
Sbjct: 149 QVCFCEKPDFVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGAL 208
Query: 194 FGYVAG 199
G G
Sbjct: 209 LGSALG 214
>gb|AAF49133.1| (AE003516) CG9376 gene product [Drosophila melanogaster]
Length = 209
Score = 147 bits (370), Expect = 1e-34
Identities = 36/126 (28%), Positives = 56/126 (43%), Gaps = 5/126 (3%)
Query: 79 RALKCCVCFLMSCFLLH-IIFVLYGAPLIELVLETFLFAVVLSTFTTVPCLCLLGPNLKA 137
R L C LL+ I ++ GAP++ +TF+ A++++ T P + LLG
Sbjct: 62 RELLGGFTLQFLCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGAL 121
Query: 138 WLRVFSRNGVTSIWE----NSLQITTISSFTGAWLGAFPIPLDWERPWQVWPISCTLGAT 193
+ + + E N + + GAW G+ PLDW R WQ +PI +GA
Sbjct: 122 QVCFCEKPDFVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGAL 181
Query: 194 FGYVAG 199
G G
Sbjct: 182 LGSALG 187
>pir||T29643 hypothetical protein F49E8.1 - Caenorhabditis elegans
Length = 571
Score = 146 bits (366), Expect = 3e-34
Identities = 36/121 (29%), Positives = 62/121 (50%), Gaps = 4/121 (3%)
Query: 80 ALKCCVCFL-MSCFLLHIIFVLYGAPLIELVLETFLFAVVLSTFTTVPCLCLLGPNLKA- 137
+L F+ +S + +I+ VL+GAP ++ T + A L+ + +P + L +A
Sbjct: 3 SLPVAARFVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAV 62
Query: 138 --WLRVFSRNGVTSIWENSLQITTISSFTGAWLGAFPIPLDWERPWQVWPISCTLGATFG 195
L++FS G + E+ L ++ +F GAW A PLDW+R WQ +P+ +G G
Sbjct: 63 EVILQLFSCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIG 122
Query: 196 Y 196
Sbjct: 123 A 123
>ref|NP_501226.1| (NM_068825) F49E8.1.p [Caenorhabditis elegans]
gb|AAB03156.2| (U61949) Hypothetical protein F49E8.1 [Caenorhabditis elegans]
Length = 553
Score = 145 bits (365), Expect = 5e-34
Identities = 36/121 (29%), Positives = 62/121 (50%), Gaps = 4/121 (3%)
Query: 80 ALKCCVCFL-MSCFLLHIIFVLYGAPLIELVLETFLFAVVLSTFTTVPCLCLLGPNLKA- 137
+L F+ +S + +I+ VL+GAP ++ T + A L+ + +P + L +A
Sbjct: 3 SLPVAARFVTLSWAVFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAV 62
Query: 138 --WLRVFSRNGVTSIWENSLQITTISSFTGAWLGAFPIPLDWERPWQVWPISCTLGATFG 195
L++FS G + E+ L ++ +F GAW A PLDW+R WQ +P+ +G G
Sbjct: 63 EVILQLFSCEGNPTPKESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIG 122
Query: 196 Y 196
Sbjct: 123 A 123
>gb|AAF18502.1|AC010924_15 (AC010924) ESTs gb|AI992787, gb|T20398 come from this gene.
[Arabidopsis thaliana]
Length = 270
Score = 133 bits (333), Expect = 3e-30
Identities = 37/124 (29%), Positives = 61/124 (48%), Gaps = 8/124 (6%)
Query: 64 PNVSSKRSSLSHKVTRALKCCVCFLMSCFLLHIIFVLYGAPL-IELVLETFLFAVVLSTF 122
P S ++ + + + + V GAP+ ++ + +T ++ ++S F
Sbjct: 79 PEKCSYFRAVGRSLVGLIAGALINALG-------AVSLGAPIGMQSLSKTIHWSFLMSVF 131
Query: 123 TTVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFTGAWLGAFPIPLDWERPWQ 182
T VP +LG + W R+F+ I E+ L + + G W GA+P+PLDWERPWQ
Sbjct: 132 TVVPATAVLGASWIDWHRIFASLKPIGIIEHMLLVPAYGAIIGGWFGAWPMPLDWERPWQ 191
Query: 183 VWPI 186
WPI
Sbjct: 192 EWPI 195
>ref|NP_173057.1| (NM_101473) hypothetical protein [Arabidopsis thaliana]
Length = 160
Score = 106 bits (264), Expect = 4e-22
Identities = 27/63 (42%), Positives = 36/63 (56%)
Query: 124 TVPCLCLLGPNLKAWLRVFSRNGVTSIWENSLQITTISSFTGAWLGAFPIPLDWERPWQV 183
VP +LG + W R+F+ I E+ L + + G W GA+P+PLDWERPWQ
Sbjct: 23 VVPATAVLGASWIDWHRIFASLKPIGIIEHMLLVPAYGAIIGGWFGAWPMPLDWERPWQE 82
Query: 184 WPI 186
WPI
Sbjct: 83 WPI 85
CPU time: 71.93 user secs. 2.18 sys. secs 74.11 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.320 0.172 0.560
Gapped
Lambda K H
0.270 0.0604 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 160751846
Number of Sequences: 887402
Number of extensions: 8420243
Number of successful extensions: 41045
Number of sequences better than 10.0: 245
Number of HSP's better than 10.0 without gapping: 38
Number of HSP's successfully gapped in prelim test: 207
Number of HSP's that attempted gapping in prelim test: 40899
Number of HSP's gapped (non-prelim): 327
length of query: 219
length of database: 277,845,442
effective HSP length: 47
effective length of query: 172
effective length of database: 236,137,548
effective search space: 40615658256
effective search space used: 40615658256
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.0 bits)
S2: 72 (32.1 bits)
