Sequences with E-value BETTER than threshold (0.002) 336 2e-91
333 2e-90
192 4e-48
167 2e-40
165 7e-40
163 3e-39
144 2e-33
132 5e-30
132 6e-30
132 7e-30
102 4e-21
88 1e-16
Alignments
>gb|AAL48412.1| (AY070790) AT13969p [Drosophila melanogaster]
Length = 236
Score = 336 bits (855), Expect = 2e-91
Identities = 209/209 (100%), Positives = 209/209 (100%)
Query: 1 MAYMQYRRNWAHLGSFWDSLVVPIVFLGELLKVVLARFYGKVEDGVLTAKQRQKKNSYFT 60
MAYMQYRRNWAHLGSFWDSLVVPIVFLGELLKVVLARFYGKVEDGVLTAKQRQKKNSYFT
Sbjct: 28 MAYMQYRRNWAHLGSFWDSLVVPIVFLGELLKVVLARFYGKVEDGVLTAKQRQKKNSYFT 87
Query: 61 PRELLGGFTLQFLCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGA 120
PRELLGGFTLQFLCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGA
Sbjct: 88 PRELLGGFTLQFLCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGA 147
Query: 121 LQVCFCEKPDFVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGA 180
LQVCFCEKPDFVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGA
Sbjct: 148 LQVCFCEKPDFVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGA 207
Query: 181 LLGSALGNIYACTHVLYATARVYMTKKRT 209
LLGSALGNIYACTHVLYATARVYMTKKRT
Sbjct: 208 LLGSALGNIYACTHVLYATARVYMTKKRT 236
>gb|AAF49133.1| (AE003516) CG9376 gene product [Drosophila melanogaster]
Length = 209
Score = 333 bits (846), Expect = 2e-90
Identities = 209/209 (100%), Positives = 209/209 (100%)
Query: 1 MAYMQYRRNWAHLGSFWDSLVVPIVFLGELLKVVLARFYGKVEDGVLTAKQRQKKNSYFT 60
MAYMQYRRNWAHLGSFWDSLVVPIVFLGELLKVVLARFYGKVEDGVLTAKQRQKKNSYFT
Sbjct: 1 MAYMQYRRNWAHLGSFWDSLVVPIVFLGELLKVVLARFYGKVEDGVLTAKQRQKKNSYFT 60
Query: 61 PRELLGGFTLQFLCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGA 120
PRELLGGFTLQFLCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGA
Sbjct: 61 PRELLGGFTLQFLCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGA 120
Query: 121 LQVCFCEKPDFVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGA 180
LQVCFCEKPDFVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGA
Sbjct: 121 LQVCFCEKPDFVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGA 180
Query: 181 LLGSALGNIYACTHVLYATARVYMTKKRT 209
LLGSALGNIYACTHVLYATARVYMTKKRT
Sbjct: 181 LLGSALGNIYACTHVLYATARVYMTKKRT 209
>pir||T40997 probable short-chain dehydrogenase - fission yeast
(Schizosaccharomyces pombe)
emb|CAB40182.1| (AL049559) putative short-chain dehydrogenase [Schizosaccharomyces
pombe]
Length = 503
Score = 192 bits (484), Expect = 4e-48
Identities = 46/130 (35%), Positives = 66/130 (50%), Gaps = 6/130 (4%)
Query: 60 TPRELLGGFTLQFLCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGG 119
TP+ LL G + +LL +FI + GAP+L ++ T+ AL +++ TV P L
Sbjct: 374 TPKRLLAGAASMLIGSLLISFILVAFGAPLLHDFHLTYFCALTLSVFTVYPLASTLAFNT 433
Query: 120 ALQVCFCEKPDFVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIG 179
+ F+T + + + G I+GAW G+ PLDW R WQA+PI VIG
Sbjct: 434 E------QWQRFLTLKSFNVIGSMQLRSWGPIIGAWFGAFPIPLDWDRPWQAWPITIVIG 487
Query: 180 ALLGSALGNI 189
A LG A I
Sbjct: 488 AFLGYAFAAI 497
>ref|NP_002634.1| (NM_002643) phosphatidylinositol glycan, class F [Homo sapiens]
sp|Q07326|PIGF_HUMAN Phosphatidylinositol-glycan biosynthesis, class F protein (PIG-F)
pir||A46097 GPI-anchor biosynthesis protein PIG-F - human
dbj|BAA02697.1| (D13435) PIG-F [Homo sapiens]
Length = 219
Score = 167 bits (419), Expect = 2e-40
Identities = 33/117 (28%), Positives = 56/117 (47%), Gaps = 5/117 (4%)
Query: 72 FLCT-LLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGALQVCFCEKPD 130
FL + + I ++ GAP++ +TF+ A++++ T P + LLG + +
Sbjct: 87 FLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNG 146
Query: 131 FVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGALLGSALG 187
+ E N + + +GAW G++ PLDW R WQ +PI +GA G G
Sbjct: 147 VTSIWE----NSLQITTISSFVGAWLGALPIPLDWERPWQVWPISCTLGATFGYVAG 199
>ref|NP_032864.1| (NM_008838) phosphatidylinositol glycan, class F [Mus musculus]
dbj|BAA08818.1| (D50264) phosphatidylinositol glycan class F [Mus musculus]
Length = 219
Score = 165 bits (414), Expect = 7e-40
Identities = 34/117 (29%), Positives = 54/117 (46%), Gaps = 5/117 (4%)
Query: 72 FLCT-LLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGALQVCFCEKPD 130
FL + L I ++ GAP++ +TF+ A++++ T P + LLG + +
Sbjct: 87 FLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLSTFTTVPCLCLLGPNLKAWLRVFSRNG 146
Query: 131 FVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGALLGSALG 187
+ E N + + GAW G+ PLDW R WQ +PI +GA G G
Sbjct: 147 VTSIWE----NSLQITTISSFTGAWLGAFPIPLDWERPWQVWPISCTLGATFGYVAG 199
>gb|AAF18502.1|AC010924_15 (AC010924) ESTs gb|AI992787, gb|T20398 come from this gene.
[Arabidopsis thaliana]
Length = 270
Score = 163 bits (409), Expect = 3e-39
Identities = 38/128 (29%), Positives = 56/128 (43%), Gaps = 11/128 (8%)
Query: 50 KQRQKKNSYFTP--RELLGGFTLQFLCTLLYAFICIILGAPV-LGNYEQTFVLALLMTLL 106
++ +K SYF R L+G L+ A + LGAP+ + + +T + LM++
Sbjct: 76 RRNPEKCSYFRAVGRSLVG----LIAGALINALGAVSLGAPIGMQSLSKTIHWSFLMSVF 131
Query: 107 TVSPTVFLLGGGGALQVCFCEKPDFVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWG 166
TV P +LG + E L A G I+G W G+ PLDW
Sbjct: 132 TVVPATAVLGASWIDWHRIFASLKPIGIIEHMLL----VPAYGAIIGGWFGAWPMPLDWE 187
Query: 167 RDWQAYPI 174
R WQ +PI
Sbjct: 188 RPWQEWPI 195
>gb|AAH21725.1|AAH21725 (BC021725) Similar to phosphatidylinositol glycan, class F [Homo
sapiens]
Length = 206
Score = 144 bits (360), Expect = 2e-33
Identities = 27/100 (27%), Positives = 48/100 (48%), Gaps = 5/100 (5%)
Query: 72 FLCT-LLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGALQVCFCEKPD 130
FL + + I ++ GAP++ +TF+ A++++ T P + LLG + +
Sbjct: 87 FLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPNLKAWLRVFSRNG 146
Query: 131 FVTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQ 170
+ E N + + +GAW G++ PLDW R WQ
Sbjct: 147 VTSIWE----NSLQITTISSFVGAWLGALPIPLDWERPWQ 182
>pir||T29643 hypothetical protein F49E8.1 - Caenorhabditis elegans
Length = 571
Score = 132 bits (331), Expect = 5e-30
Identities = 38/113 (33%), Positives = 58/113 (50%), Gaps = 11/113 (9%)
Query: 77 LYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGG-----ALQVCFCEKPDF 131
++ + ++ GAP + T VLA +T ++ P VFL LQ+ CE
Sbjct: 17 VFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLFSCEGNP- 75
Query: 132 VTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGALLGS 184
T E L +N++ LGAWA + V PLDW R WQ YP+P+++G +G+
Sbjct: 76 -TPKESVLL----FNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIGA 123
>ref|NP_010588.1| (NC_001136) Glycosylphosphatidylinositol (GPI) assembly; Gpi11p
[Saccharomyces cerevisiae]
pir||S61188 probable membrane protein YDR302w - yeast (Saccharomyces
cerevisiae)
gb|AAB64738.1| (U28374) Ydr302wp [Saccharomyces cerevisiae]
Length = 219
Score = 132 bits (329), Expect = 6e-30
Identities = 37/116 (31%), Positives = 56/116 (47%), Gaps = 19/116 (16%)
Query: 72 FLCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGALQVCFCEKPDF 131
FL + + I+ GAP++ +T++L+L L P V+
Sbjct: 116 FLLSFPTMLLTILFGAPLMDLLWETWLLSLHFAFLAY-PAVY-----------------S 157
Query: 132 VTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGALLGSALG 187
V C D + L+K + ++G W VV PLDW RDWQ +PIP V+G LG+ +G
Sbjct: 158 VFNC-DFKVGLWKKYFIFIVVGGWISCVVIPLDWDRDWQNWPIPIVVGGYLGALVG 212
Score = 93.9 bits (231), Expect = 2e-18
Identities = 32/99 (32%), Positives = 45/99 (45%), Gaps = 8/99 (8%)
Query: 96 TFVLALLMTLLTVSPTVFLLGGGGALQ----VCFCEKPD---FVTKCEDTALNLFKYNAL 148
TF+L+ LLT+ L+ V C D + L+K +
Sbjct: 115 TFLLSFPTMLLTILFGAPLMDLLWETWLLSLHFAFLAYPAVYSVFNC-DFKVGLWKKYFI 173
Query: 149 GGILGAWAGSVVAPLDWGRDWQAYPIPNVIGALLGSALG 187
++G W VV PLDW RDWQ +PIP V+G LG+ +G
Sbjct: 174 FIVVGGWISCVVIPLDWDRDWQNWPIPIVVGGYLGALVG 212
>ref|NP_501226.1| (NM_068825) F49E8.1.p [Caenorhabditis elegans]
gb|AAB03156.2| (U61949) Hypothetical protein F49E8.1 [Caenorhabditis elegans]
Length = 553
Score = 132 bits (329), Expect = 7e-30
Identities = 38/113 (33%), Positives = 58/113 (50%), Gaps = 11/113 (9%)
Query: 77 LYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGG-----ALQVCFCEKPDF 131
++ + ++ GAP + T VLA +T ++ P VFL LQ+ CE
Sbjct: 17 VFYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLFSCEGNP- 75
Query: 132 VTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGALLGS 184
T E L +N++ LGAWA + V PLDW R WQ YP+P+++G +G+
Sbjct: 76 -TPKESVLL----FNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIGA 123
>ref|NP_173057.1| (NM_101473) hypothetical protein [Arabidopsis thaliana]
Length = 160
Score = 102 bits (254), Expect = 4e-21
Identities = 23/79 (29%), Positives = 29/79 (36%), Gaps = 4/79 (5%)
Query: 96 TFVLALLMTLLTVSPTVFLLGGGGALQVCFCEKPDFVTKCEDTALNLFKYNALGGILGAW 155
T + L V P +LG + E L A G I+G W
Sbjct: 11 TSAWRFVHDLKDVVPATAVLGASWIDWHRIFASLKPIGIIEHMLL----VPAYGAIIGGW 66
Query: 156 AGSVVAPLDWGRDWQAYPI 174
G+ PLDW R WQ +PI
Sbjct: 67 FGAWPMPLDWERPWQEWPI 85
>ref|XP_018442.1| (XM_018442) similar to phosphatidylinositol glycan, class F (H.
sapiens) [Homo sapiens]
Length = 153
Score = 88.4 bits (217), Expect = 1e-16
Identities = 14/65 (21%), Positives = 31/65 (47%), Gaps = 1/65 (1%)
Query: 72 FLCT-LLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGALQVCFCEKPD 130
FL + + I ++ GAP++ +TF+ A++++ T+ P + LLG + +
Sbjct: 87 FLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTIGPYLCLLGPNLKAWLRVFSRNG 146
Query: 131 FVTKC 135
+
Sbjct: 147 VTSIW 151
CPU time: 65.11 user secs. 2.63 sys. secs 67.74 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.318 0.162 0.511
Gapped
Lambda K H
0.270 0.0569 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 140860497
Number of Sequences: 887402
Number of extensions: 6986581
Number of successful extensions: 33853
Number of sequences better than 10.0: 91
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 70
Number of HSP's that attempted gapping in prelim test: 33788
Number of HSP's gapped (non-prelim): 108
length of query: 209
length of database: 277,845,442
effective HSP length: 48
effective length of query: 161
effective length of database: 235,250,146
effective search space: 37875273506
effective search space used: 37875273506
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.0 bits)
S2: 72 (32.2 bits)
