PIG-C: orf6.6750.prot BLAST Search Results

GPI Biosynthesis Report

GPI Anchor Biosynthesis Report: orf6.6750.prot (PIG-C family, Candida albicans)

BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (304 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!

 Results of PSI-Blast iteration 1

Distribution of 11 Blast Hits on the Query Sequence

Sequences with E-value BETTER than threshold (0.002)

Sequences producing significant alignments:


 dbj|BAB29152.1|  (AK014096) data source:SPTR, source key:Q92535, ...   325  5e-88

 ref|NP_002633.1|  (NM_002642) phosphatidylinositol glycan, class ...   324  1e-87

 dbj|BAA22866.1|  (AB000360) PIGC [Homo sapiens]                        320  3e-86

 pir||T41379  probable phosphatidylinositol- glycan-class c protei...   314  1e-84

 ref|NP_501645.1|  (NM_069244) T20D3.8.p [Caenorhabditis elegans] ...   273  2e-72

 gb|AAF47758.1|  (AE003477) CG12077 gene product [Drosophila melan...   269  3e-71

 ref|NP_181043.1|  (NM_129050) putative phosphatidylinositol-glyca...   248  9e-65

 ref|NP_015249.1|  (NC_001148) Required for synthesis of N-acetylg...   241  1e-62

 gb|AAA79518.1|  (U23788) Gpi2p [Saccharomyces cerevisiae]              216  5e-55

Sequences with E-value WORSE than threshold 

 ref|NP_359627.1|  (NC_003098) Conserved hypothetical protein [Str...    35  2.1

 ref|NP_346639.1|  (NC_003028) ABC transporter, permease protein, ...    35  2.1

Alignments
>dbj|BAB29152.1| (AK014096) data source:SPTR, source key:Q92535,
           evidence:ISS~homolog to PHOSPHATIDYLINOSITOL-GLYCAN
           BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)~putative [Mus
           musculus]
 gb|AAH06938.1|AAH06938 (BC006938) RIKEN cDNA 3110030E07 gene [Mus musculus]
          Length = 297

 Score =  325 bits (826), Expect = 5e-88
 Identities = 92/297 (30%), Positives = 151/297 (49%), Gaps = 34/297 (11%)

Query: 23  QHAQQQEQPSWKKLLYLQQPFPDNYTDQSFLSQLKRNTTVAKYSYKNLVQAFSFIVFYIS 82
           Q      +  W+K+LY +QPFPDNY DQ FL +L++N    KY Y  +V   S ++  + 
Sbjct: 4   QRVTDTPEVKWQKVLYERQPFPDNYVDQRFLEELRKNIYARKYQYWAVVFESSVVIQQLC 63

Query: 83  --CILLVILMFI--GIYEKQWDPIIPTLISTGI--SLVGFF-------------SLKNLS 123
             C+ +VI  ++  G+   QW      L  TG+  SLVG+              S +   
Sbjct: 64  SVCVFVVIWWYMDEGLLAPQW------LFGTGLASSLVGYVLFDLIDGGDGRKKSGRTRW 117

Query: 124 MNMRSLMVITFILLIVSPILKSLTKSTSSDSIWAISCVLCLANSIFHDYSMKVSYRPIVS 183
            +++S +V        SP+LK+LT+S S+D+I+A++  + L + IF DY    +   IVS
Sbjct: 118 ADLKSTLVFITFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANAA---IVS 174

Query: 184 TNISLSNAI----VLASRLSTTLDVFLFVLFAIEVNILLPLFDAKIRLSGLETIHWPIAF 239
           + +SL+ AI     LASRL  +L  F+ V FAI++  L P+   K++     +       
Sbjct: 175 STLSLNMAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSY--VGVT 232

Query: 240 LTFNLTSYLMYILLNYKFLVYWILSVFVMVVIMPIYFLSLQRYKNEIQGPWDVAKPK 296
           L F  +++   + ++    + + L +F +  + P Y + LQ +K  I GPWD A+ K
Sbjct: 233 LLFAFSAFGGLLSISAVGAILFALLLFSISCLCPYYLIHLQLFKENIHGPWDEAEIK 289



>ref|NP_002633.1| (NM_002642) phosphatidylinositol glycan, class C [Homo sapiens]
 ref|XP_002139.1| (XM_002139) phosphatidylinositol glycan, class C [Homo sapiens]
 sp|Q92535|PIGC_HUMAN PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)
 pir||JC4969 pig-c protein - human
 dbj|BAA12812.1| (D85418) phosphatidylinositol-glycan-class C (PIG-C) [Homo sapiens]
 emb|CAB10071.1| (Z97195) PHOSPHATIDYLINISITOL-GLYCAN class C (PIG-C) [Homo sapiens]
 gb|AAH06539.1|AAH06539 (BC006539) phosphatidylinositol glycan, class C [Homo sapiens]
          Length = 297

 Score =  324 bits (823), Expect = 1e-87
 Identities = 90/297 (30%), Positives = 150/297 (50%), Gaps = 34/297 (11%)

Query: 23  QHAQQQEQPSWKKLLYLQQPFPDNYTDQSFLSQLKRNTTVAKYSYKNLVQAFSFIVFYIS 82
           Q     ++  W+K+LY +QPFPDNY D+ FL +L++N    KY Y  +V   S ++  + 
Sbjct: 4   QPVTNTKEVKWQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQQLC 63

Query: 83  --CILLVILMFI--GIYEKQWDPIIPTLISTGI--SLVGFF-------------SLKNLS 123
             C+ +VI  ++  G+    W      L+ TG+  SL+G+              S +   
Sbjct: 64  SVCVFVVIWWYMDEGLLAPHW------LLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRW 117

Query: 124 MNMRSLMVITFILLIVSPILKSLTKSTSSDSIWAISCVLCLANSIFHDYSMKVSYRPIVS 183
            +++S +V        SP+LK+LT+S S+D+I+A+S  + L + IF DY    +   IVS
Sbjct: 118 ADLKSALVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAA---IVS 174

Query: 184 TNISLSNAI----VLASRLSTTLDVFLFVLFAIEVNILLPLFDAKIRLSGLETIHWPIAF 239
           + +SL+ AI     LASRL  +L  F+ V FAI++  L P+   K++     +       
Sbjct: 175 STLSLNMAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSY--VGVT 232

Query: 240 LTFNLTSYLMYILLNYKFLVYWILSVFVMVVIMPIYFLSLQRYKNEIQGPWDVAKPK 296
           L F  ++    + ++    V + L +  +  + P Y + LQ +K  I GPWD A+ K
Sbjct: 233 LLFAFSAVGGLLSISAVGAVLFALLLMSISCLCPFYLIRLQLFKENIHGPWDEAEIK 289



>dbj|BAA22866.1| (AB000360) PIGC [Homo sapiens]
          Length = 297

 Score =  320 bits (812), Expect = 3e-86
 Identities = 89/297 (29%), Positives = 149/297 (49%), Gaps = 34/297 (11%)

Query: 23  QHAQQQEQPSWKKLLYLQQPFPDNYTDQSFLSQLKRNTTVAKYSYKNLVQAFSFIVFYIS 82
           Q     ++  W+K+LY +QPFPDNY D+ FL +L++N    KY Y  +V   S ++  + 
Sbjct: 4   QPVTNTKEVKWQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQQLC 63

Query: 83  --CILLVILMFI--GIYEKQWDPIIPTLISTGI--SLVGFF-------------SLKNLS 123
             C+ +VI  ++  G+    W      L+ TG+  SL+G+              S +   
Sbjct: 64  SVCVFVVIWWYMDEGLLAPHW------LLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRW 117

Query: 124 MNMRSLMVITFILLIVSPILKSLTKSTSSDSIWAISCVLCLANSIFHDYSMKVSYRPIVS 183
            +++S +V        SP+LK+LT+S S+D+I+A+S  + L + IF DY    +   IVS
Sbjct: 118 ADLKSALVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAA---IVS 174

Query: 184 TNISLSNAI----VLASRLSTTLDVFLFVLFAIEVNILLPLFDAKIRLSGLETIHWPIAF 239
           + +SL+ AI     LASRL  +L  F+ V FAI++  L P+   K++     +       
Sbjct: 175 STLSLNMAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSY--VGVT 232

Query: 240 LTFNLTSYLMYILLNYKFLVYWILSVFVMVVIMPIYFLSLQRYKNEIQGPWDVAKPK 296
           L F  ++    + ++    V + L +  +  +   Y + LQ +K  I GPWD A+ K
Sbjct: 233 LLFAFSAVGGLLSISAVGAVLFALLLMSISCLCSFYLIRLQLFKENIHGPWDEAEIK 289



>pir||T41379 probable phosphatidylinositol- glycan-class c protein - fission
           yeast (Schizosaccharomyces pombe)
 emb|CAA19108.1| (AL023592) phosphatidylinositol-glycan-class c [Schizosaccharomyces
           pombe]
          Length = 324

 Score =  314 bits (797), Expect = 1e-84
 Identities = 101/301 (33%), Positives = 163/301 (53%), Gaps = 25/301 (8%)

Query: 20  NHFQHAQQQE----QPSWKKLLYLQQPFPDNYTDQSFLSQLKRNTTVAKYSYKNLVQAFS 75
           N F+ A  +E    +  WKK+L+ +Q +PDN+ D+SFL+ L+RN  +    + +LV    
Sbjct: 21  NTFRKASVKEVVELKKPWKKVLWRKQDYPDNFIDESFLNGLQRNVNIQVTDFWSLVADSL 80

Query: 76  FIVFYISCILLVILMFIGIYEKQWDPIIPTLISTGISLVGF----FSLKNLSMN------ 125
            +  ++S +++   +F+ IY  Q    +   +S   ++  F    F L+    N      
Sbjct: 81  PVSQHLSSVVIFASVFVSIYRNQLSCALVGFVSNVSAVAAFILWDFVLRKPCNNRTFPNY 140

Query: 126 ---MRSLMVITFILLIVSPILKSLTKSTSSDSIWAISCVLCLANSIFHDYSMKVSYR--- 179
              ++S ++I   L  +SPIL SLTKSTS DS+WAI+  L LAN  FH+Y+ +       
Sbjct: 141 MGIVKSCILIVLTLAGLSPILMSLTKSTSPDSVWAIAVWLFLANVFFHEYTTETIRPHVR 200

Query: 180 --PIVSTNISLSNAIVLASRLSTTLDVFLFVLFAIEVNILLPLFDAKIRLSGLETIHWPI 237
               +STN +LS ++VLASRL  +++VF F+LFA+    L P+F   I +         +
Sbjct: 201 LHNSLSTNAALSASVVLASRLEKSINVFFFILFAVHWFALFPIFRKYIHVFSFYADM--L 258

Query: 238 AFLTFNLTSYLMYILLNYKFLVYWIL-SVFVMVVIMPIYFLSLQRYKNEIQGPWDVAKPK 296
             L   +++Y+    +    + +  L  +F +  I PI+F+ LQR+KNEI GPWD+A PK
Sbjct: 259 MTLVLIISAYIALNAVASVVIAFVFLSLIFFISFICPIWFIKLQRFKNEIHGPWDIALPK 318

Query: 297 L 297
           L
Sbjct: 319 L 319



>ref|NP_501645.1| (NM_069244) T20D3.8.p [Caenorhabditis elegans]
 pir||T25032 hypothetical protein T20D3.8 - Caenorhabditis elegans
 emb|CAA92490.2| (Z68220) cDNA EST yk416d12.3 comes from this gene~cDNA EST
           yk416d12.5 comes from this gene~cDNA EST yk586b10.3
           comes from this gene~cDNA EST yk586b10.5 comes from this
           gene [Caenorhabditis elegans]
          Length = 282

 Score =  273 bits (693), Expect = 2e-72
 Identities = 80/278 (28%), Positives = 137/278 (48%), Gaps = 22/278 (7%)

Query: 33  WKKLLYLQQPFPDNYT--DQSFLSQLKRNTTVAKYSYKNLVQAFSFIVFYISCILLVILM 90
           W+K+LY +QPFPDNY+  D  FL +L++N +V  Y YK+ V      + ++  I +  ++
Sbjct: 7   WQKILYRKQPFPDNYSGGDAQFLKELRKNVSVVHYDYKSAVFGCMNFLTHLDMITMYFVL 66

Query: 91  FIGIYEKQWD-PIIPTLISTGISLVGFFS------LKNLSMNMRSLMVITFILLIVSPIL 143
           F+ I    W   I+ ++ S  I L  FF         N   + R++  +       +P++
Sbjct: 67  FLNILHSNWSINILYSVFSLTIVLYLFFCKFLIPNPANAKEHARTIFTLFIFAYAFTPVI 126

Query: 144 KSLTKSTSSDSIWAISCVLCLANSIFHDYSMK---VSYRPIVSTNISLSNAIVLASRLST 200
           ++LT S S+D+I++ S +  + +  FHDY +K   VSY   VST   LS+AI L SRL  
Sbjct: 127 RTLTTSISTDTIYSTSIITAIFSCFFHDYGVKAPVVSYPTSVST--GLSSAIFLLSRLEG 184

Query: 201 TLDVFLFVLFAIEVNILLPLFDAKIRLSGLETIHWPIAFLTFNLTSYLMYILLNYKF--- 257
                L ++ A      L  + A+ R           + +   L+ + +Y + ++     
Sbjct: 185 DTPTLLLLVVAFT----LHAYGAEFRNRIFHVYPCLSSTIFCFLSLFSIYCISDFSLELS 240

Query: 258 LVYWILSVFVMVVIMPIYFLSLQRYKNEIQGPWDVAKP 295
           + + +L +F++  I P+  +  Q  K  I GPWD A P
Sbjct: 241 ICFALLHIFIL-FICPLILILKQTGKCTIHGPWDEAVP 277



>gb|AAF47758.1| (AE003477) CG12077 gene product [Drosophila melanogaster]
          Length = 256

 Score =  269 bits (683), Expect = 3e-71
 Identities = 71/284 (25%), Positives = 121/284 (42%), Gaps = 57/284 (20%)

Query: 23  QHAQQQEQPSWKKLLYLQQPFPDNYTDQSFLSQLKRNTTVAKYSYKNLVQAFSFIVFYIS 82
           ++ Q + QP W K LY  + +PDNYTD SFL  L+ N     Y++   +   + +   IS
Sbjct: 3   KNGQSKRQP-WVKNLYSNREYPDNYTDASFLKDLRTNLHCRIYTFGEAIAGITVLNNQIS 61

Query: 83  CILLVILMFIGIYEKQWDPIIPTLISTGISLVGFFSLKNLS-------MNMRSLMVITFI 135
           CI   ++++  +      P    + S GI+ +G+   +  S        + ++L+ +   
Sbjct: 62  CITGFLILYQLMLSDSVSPTTILVPSCGITGIGYLCYRGRSLSWALLGEDSKTLVTVVLF 121

Query: 136 LLIVSPILKSLTKSTSSDSIWAISCVLCLANSIFHDYSMKVSYRPIVSTNISLSNAIVLA 195
             + SP+L +LT++ S+D+I+ ++  + L N IF  Y + V+          +S A V  
Sbjct: 122 GYLFSPMLHTLTQAISTDTIYTMTFFVLLGNLIFGHYGLDVAM---------VSKAAV-- 170

Query: 196 SRLSTTLDVFLFVLFAI------EVNILLPLFDAKIRLSGLETIHWPIAFLTFNLTSYLM 249
                      FVL+ I          +LP+F   I    L  I  P+ +L  + T    
Sbjct: 171 ----------FFVLYPIMTEANWHAGFMLPIF--AICCMALYCISRPVLYLYASTT---- 214

Query: 250 YILLNYKFLVYWILSVFVMVVIMPIYFLSLQRYKNEIQGPWDVA 293
                             +  + P  F+  Q+YK  I GPWD A
Sbjct: 215 ----------------IFINFVCPFIFVWQQQYKFNIHGPWDEA 242



>ref|NP_181043.1| (NM_129050) putative phosphatidylinositol-glycan synthase
           [Arabidopsis thaliana]
 pir||T00479 hypothetical protein F19I3.21 - Arabidopsis thaliana
 gb|AAC12837.1| (AC004238) putative phosphatidylinositol-glycan synthase
           [Arabidopsis thaliana]
          Length = 303

 Score =  248 bits (628), Expect = 9e-65
 Identities = 90/292 (30%), Positives = 144/292 (48%), Gaps = 24/292 (8%)

Query: 25  AQQQEQPSWKKLLY--LQQPFPDNYTDQSFLSQLKRNTTVAKYSYKNLVQAFSFIVFYIS 82
                 P W+K+ Y  +Q  + DNYTD+SFL ++  N  V +     +++    I  Y+ 
Sbjct: 7   GDSIPGPKWRKVAYGGMQIGYDDNYTDESFLEEMVMNANVVRRDLLKVMKDSVSISQYLC 66

Query: 83  CILLVILMFIGIYEKQWDPIIPTLISTGISLVGFFSLKNLSMNM-------RSLMVITFI 135
            + LV+L+++   E   D     L+   +   GF  L      M       R L+ I+F 
Sbjct: 67  IVALVVLVWVHTLESSLDENSLLLLDLSLLASGFLILLLTEEKMLSLSLLLRYLVNISFF 126

Query: 136 ---LLIVSPILKSLTKSTSSDSIWAISCVLCLANSIFHDYSMKVSYRP----------IV 182
              L I++PI ++LT+S SSDSIWA++  L L +   HDYS      P           +
Sbjct: 127 TTGLYILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTPNLTSCI 186

Query: 183 STNISLSNAIVLASRLSTTLDVFLFVLFAIEVNILLPLFDAKIRLSGLETIHWPIAFLTF 242
           S N S+  ++ +ASRL + L VF  +LF+++V +  PL    I+      +H   +F   
Sbjct: 187 SVNASIVASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTYCIKKFNF-GLHLLFSFALM 245

Query: 243 NLTSYLMYILLNYKFLVYWILSVFVMVVIMPIYFLSLQRYKNEIQGPWDVAK 294
            LT Y +Y  L+  F + ++  V ++ V+ P + + +Q YK EI GPWD AK
Sbjct: 246 GLTLYSIY-ALHRLFFLVFLSLVLLVNVVCPYWLIRMQEYKFEINGPWDEAK 296



>ref|NP_015249.1| (NC_001148) Required for synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi2p
           [Saccharomyces cerevisiae]
 sp|P46961|GPI2_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI2
 pir||S61111 GPI2 protein - yeast (Saccharomyces cerevisiae)
 dbj|BAA06128.1| (D29645) Gcr4p [Saccharomyces cerevisiae]
 gb|AAB68262.1| (U41849) Gpi2p [Saccharomyces cerevisiae]
          Length = 280

 Score =  241 bits (611), Expect = 1e-62
 Identities = 91/284 (32%), Positives = 154/284 (54%), Gaps = 27/284 (9%)

Query: 32  SWKKLLYLQQPFPDNYTDQSFLSQLKRNTTVA-KYSYKNLVQA------FSFIVFYISCI 84
            WK+LL+L+Q +PDNYTD SF+    R    + + S + L +A        FI FY + +
Sbjct: 5   PWKRLLWLKQEYPDNYTDPSFIELRARQKAESNQKSDRKLSEAARAQIRLDFISFYQTIL 64

Query: 85  --LLVILMFIGIYEKQWDPIIPTLISTGISLV----GFFSLKNLSMNMRSLMVITFILLI 138
               + + F  IY   +DPI PT+  + I+L+        L +  M+++S ++ITF +L 
Sbjct: 65  NTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLIITFAMLT 124

Query: 139 VSPILKSLTKSTSSDSIWAISCVLCLANSIFHDYSMKVSYRPI-VSTNISLSNAIVLASR 197
           +SP+LKSL+K+T+SDSIW +S  L L   IF   S K   +P  +STNI ++   VL+SR
Sbjct: 125 LSPVLKSLSKTTASDSIWTLSFWLTLW-YIFVISSTKSKDKPSNLSTNILVALVAVLSSR 183

Query: 198 LSTTLDVFLFVLFAIEVNILLPLFDAKIRLSGLETIHWPIAFLTFNLTSYLMYILLNYKF 257
           LSTT+DVF F+L  I++NI+LP +        L   +  +  ++  +    + + L + +
Sbjct: 184 LSTTIDVFCFLLICIQLNIILPTY--------LSVTNKVVPIISNIIVYSFLNVALGWIY 235

Query: 258 LVYWILSVFVMVVIMPIYF----LSLQRYKNEIQGPWDVAKPKL 297
           ++    +    + ++P +F    ++  +  N++   WD   P L
Sbjct: 236 MLLIFFASVFYITVLPKWFIYWKINYHKRDNDLLSTWDARTPIL 279



>gb|AAA79518.1| (U23788) Gpi2p [Saccharomyces cerevisiae]
          Length = 268

 Score =  216 bits (545), Expect = 5e-55
 Identities = 92/250 (36%), Positives = 144/250 (56%), Gaps = 17/250 (6%)

Query: 32  SWKKLLYLQQPFPDNYTDQSFLSQLKRNTTVA-KYSYKNLVQA------FSFIVFYISCI 84
            WK+LL+L+Q +PDNYTD SF+    R    + + S + L +A        FI FY + +
Sbjct: 5   PWKRLLWLKQEYPDNYTDPSFIELRARQKAESNQKSDRKLSEARRAQIRLDFISFYQTIL 64

Query: 85  --LLVILMFIGIYEKQWDPIIPTLISTGISLV----GFFSLKNLSMNMRSLMVITFILLI 138
               + + F  IY   +DPI PT+  + I+L+        L +  M+++S ++ITF +L 
Sbjct: 65  NTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLIITFAMLT 124

Query: 139 VSPILKSLTKSTSSDSIWAISCVLCLANSIFHDYSMKVSYRPI-VSTNISLSNAIVLASR 197
           +SP+LKSL+K+T+SDSIW +S  L L   IF   S K   +P  +STNI ++   VL+SR
Sbjct: 125 LSPVLKSLSKTTASDSIWTLSFWLTLW-YIFVISSTKSKDKPSNLSTNILVALVAVLSSR 183

Query: 198 LSTTLDVFLFVLFAIEVNILLPLFDAKIRLSGLETIHWPIAFLTFNLTSYLMYILLNYKF 257
           LSTT+DVF F+L  I++NI+LP +   +    +  I   I +   N+    +Y+LL +  
Sbjct: 184 LSTTIDVFCFLLICIQLNIILPTY-LSVTNKVVPIISNIIVYSFLNVALGWIYMLL-FSL 241

Query: 258 LVYWILSVFV 267
           L Y+IL  ++
Sbjct: 242 LQYFILQFYL 251



>ref|NP_359627.1| (NC_003098) Conserved hypothetical protein [Streptococcus
           pneumoniae R6]
 gb|AAL00838.1| (AE008567) Conserved hypothetical protein [Streptococcus pneumoniae
           R6]
          Length = 850

 Score = 34.5 bits (79), Expect = 2.1
 Identities = 38/194 (19%), Positives = 74/194 (37%), Gaps = 28/194 (14%)

Query: 90  MFIGIYEKQW---------DPIIPTLIST--GISLVGFFSLKNLSMNMRSLMVITFILLI 138
            ++ ++ KQ+           I    +     I  + FF+LK++  +++ + VI F  LI
Sbjct: 269 WYLDLFAKQFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLI 328

Query: 139 VSPILKSLTKSTSSDSIWAISCVLCLANSIFHDYSMKVSYRPIVSTNISLSNAIVLASRL 198
            S  +++L      D  W     +   N   H Y        I ST +  + A VL    
Sbjct: 329 ASFYIEAL------DLFWQ---GMHTPNMFLHRY------AWIFSTLLIYTAAEVLKRLK 373

Query: 199 STTLDVFLFVLFAIEVNILLPLFDAKIRLSGLETIHWPIAFLTFNLTSYLMYILLNYKFL 258
              +  FL  LF +    L  ++          T    +  L F +   L+ + +  KF+
Sbjct: 374 ELKVWNFLVSLFLVVAGFLATIYLKS--HYSFLTDLNILLTLEFLVVYSLLLLAVIKKFI 431

Query: 259 VYWILSVFVMVVIM 272
              + ++ + + I+
Sbjct: 432 SVNLFAILISLFIL 445



>ref|NP_346639.1| (NC_003028) ABC transporter, permease protein, putative
           [Streptococcus pneumoniae TIGR4]
 gb|AAK76279.1| (AE007510) ABC transporter, permease protein, putative
           [Streptococcus pneumoniae TIGR4]
          Length = 850

 Score = 34.5 bits (79), Expect = 2.1
 Identities = 38/194 (19%), Positives = 74/194 (37%), Gaps = 28/194 (14%)

Query: 90  MFIGIYEKQW---------DPIIPTLIST--GISLVGFFSLKNLSMNMRSLMVITFILLI 138
            ++ ++ KQ+           I    +     I  + FF+LK++  +++ + VI F  LI
Sbjct: 269 WYLDLFAKQFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLI 328

Query: 139 VSPILKSLTKSTSSDSIWAISCVLCLANSIFHDYSMKVSYRPIVSTNISLSNAIVLASRL 198
            S  +++L      D  W     +   N   H Y        I ST +  + A VL    
Sbjct: 329 ASFYIEAL------DLFWQ---GMHTPNMFLHRY------AWIFSTLLIYTAAEVLKRLK 373

Query: 199 STTLDVFLFVLFAIEVNILLPLFDAKIRLSGLETIHWPIAFLTFNLTSYLMYILLNYKFL 258
              +  FL  LF +    L  ++          T    +  L F +   L+ + +  KF+
Sbjct: 374 ELKVWNFLVSLFLVVAGFLATIYLKS--HYSFLTDLNILLTLEFLVVYSLLLLAVIKKFI 431

Query: 259 VYWILSVFVMVVIM 272
              + ++ + + I+
Sbjct: 432 SVNLFAILISLFIL 445



CPU time:   100.74 user secs.	    2.35 sys. secs	  103.09 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.317    0.175    0.510 

Gapped
Lambda     K      H
   0.270   0.0615    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 210128978
Number of Sequences: 887402
Number of extensions: 10859527
Number of successful extensions: 55134
Number of sequences better than 10.0: 564
Number of HSP's better than 10.0 without gapping: 40
Number of HSP's successfully gapped in prelim test: 546
Number of HSP's that attempted gapping in prelim test: 54183
Number of HSP's gapped (non-prelim): 1143
length of query: 304
length of database: 277,845,442
effective HSP length: 55
effective length of query: 249
effective length of database: 229,038,332
effective search space: 57030544668
effective search space used: 57030544668
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.8 bits)
S2: 73 (32.5 bits)