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GPI Anchor Biosynthesis Report: TRYP10.0.001875_6 (PIG-C family, Trypanosoma brucei)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (342 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 2

Distribution of 12 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)
Sequences producing significant alignments:
ref|NP_002633.1| (NM_002642) phosphatidylinositol glycan, class ... 360 2e-98
dbj|BAB29152.1| (AK014096) data source:SPTR, source key:Q92535, ... 359 4e-98
dbj|BAA22866.1| (AB000360) PIGC [Homo sapiens] 356 4e-97
pir||T41379 probable phosphatidylinositol- glycan-class c protei... 331 2e-89
ref|NP_501645.1| (NM_069244) T20D3.8.p [Caenorhabditis elegans] ... 296 4e-79
gb|AAF47758.1| (AE003477) CG12077 gene product [Drosophila melan... 275 9e-73
ref|NP_181043.1| (NM_129050) putative phosphatidylinositol-glyca... 253 5e-66
ref|NP_015249.1| (NC_001148) Required for synthesis of N-acetylg... 246 6e-64
gb|AAA79518.1| (U23788) Gpi2p [Saccharomyces cerevisiae] 229 6e-59
Sequences with E-value WORSE than threshold

ref|NP_491651.1| (NM_059250) collagen [Caenorhabditis elegans] >... 41 0.033
pir||S22041 hypothetical protein 873 - common sunflower >gi|1299... 36 1.0
ref|NP_039884.1| (NC_001345) BBRF3 late reading frame [Human her... 35 2.9
Alignments
>ref|NP_002633.1| (NM_002642) phosphatidylinositol glycan, class C [Homo sapiens]
 ref|XP_002139.1| (XM_002139) phosphatidylinositol glycan, class C [Homo sapiens]
 sp|Q92535|PIGC_HUMAN PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)
 pir||JC4969 pig-c protein - human
 dbj|BAA12812.1| (D85418) phosphatidylinositol-glycan-class C (PIG-C) [Homo sapiens]
 emb|CAB10071.1| (Z97195) PHOSPHATIDYLINISITOL-GLYCAN class C (PIG-C) [Homo sapiens]
 gb|AAH06539.1|AAH06539 (BC006539) phosphatidylinositol glycan, class C [Homo sapiens]
          Length = 297

 Score =  360 bits (916), Expect = 2e-98
 Identities = 86/286 (30%), Positives = 149/286 (52%), Gaps = 12/286 (4%)

Query: 41  VWRKVLYTRQPYEDNYVDPQQFLQDLRQNVNVATYEYNKVVLDTFVVIQQFSFVVFYLFV 100
            W+KVLY RQP+ DNYVD ++FL++LR+N++   Y+Y  VV ++ VVIQQ   V  ++ +
Sbjct: 13  KWQKVLYERQPFPDNYVD-RRFLEELRKNIHARKYQYWAVVFESSVVIQQLCSVCVFVVI 71

Query: 101 FSMMLSGRVDECALMWVNATLFTLAFVFCIVVQRQQAVDRGEIPTPFTHYLMGLCRQGIP 160
           +  M  G +   A  W+  T    + +  ++      +D GE             +  + 
Sbjct: 72  WWYMDEGLL---APHWLLGTGLASSLIGYVLF---DLIDGGEGRKKSGQTRWADLKSALV 125

Query: 161 LVGVLILLSPVLQTLTVAYSNDTIVTLSSLSMFVHVLLTDYNYLNCYSERYQQNTAVNAA 220
            +      SPVL+TLT + S DTI  +S   +  H++  DY      +       ++N A
Sbjct: 126 FITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDY---GANAAIVSSTLSLNMA 182

Query: 221 TFGIILIASRIQRVFQSGALIMFGILCFTLSPIPRHNLKHVSLRAHVVLTFTICALAIYF 280
            F  + +ASR+ R   +  ++ F I  F L P+ +  LK  + R++V +T      A+  
Sbjct: 183 IFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAVGG 242

Query: 281 LMEV-PVFALLYCVVVVVISVVIPFFFVKLHGSMKDQINGPWDEAK 325
           L+ +  V A+L+ ++++ IS + PF+ ++L    K+ I+GPWDEA+
Sbjct: 243 LLSISAVGAVLFALLLMSISCLCPFYLIRLQ-LFKENIHGPWDEAE 287
>dbj|BAB29152.1| (AK014096) data source:SPTR, source key:Q92535,
           evidence:ISS~homolog to PHOSPHATIDYLINOSITOL-GLYCAN
           BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)~putative [Mus
           musculus]
 gb|AAH06938.1|AAH06938 (BC006938) RIKEN cDNA 3110030E07 gene [Mus musculus]
          Length = 297

 Score =  359 bits (913), Expect = 4e-98
 Identities = 85/286 (29%), Positives = 145/286 (49%), Gaps = 12/286 (4%)

Query: 41  VWRKVLYTRQPYEDNYVDPQQFLQDLRQNVNVATYEYNKVVLDTFVVIQQFSFVVFYLFV 100
            W+KVLY RQP+ DNYVD Q+FL++LR+N+    Y+Y  VV ++ VVIQQ   V  ++ +
Sbjct: 13  KWQKVLYERQPFPDNYVD-QRFLEELRKNIYARKYQYWAVVFESSVVIQQLCSVCVFVVI 71

Query: 101 FSMMLSGRVDECALMWVNATLFTLAFVFCIVVQRQQAVDRGEIPTPFTHYLMGLCRQGIP 160
           +  M  G +   A  W+  T    + V  ++      +D G+             +  + 
Sbjct: 72  WWYMDEGLL---APQWLFGTGLASSLVGYVLF---DLIDGGDGRKKSGRTRWADLKSTLV 125

Query: 161 LVGVLILLSPVLQTLTVAYSNDTIVTLSSLSMFVHVLLTDYNYLNCYSERYQQNTAVNAA 220
            +      SPVL+TLT + S DTI  ++   +  H++  DY      +       ++N A
Sbjct: 126 FITFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDY---GANAAIVSSTLSLNMA 182

Query: 221 TFGIILIASRIQRVFQSGALIMFGILCFTLSPIPRHNLKHVSLRAHVVLTFTICALAIYF 280
            F  + +ASR+ R   +  ++ F I  F L P+ +  LK  + R++V +T      A   
Sbjct: 183 IFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAFSAFGG 242

Query: 281 LMEV-PVFALLYCVVVVVISVVIPFFFVKLHGSMKDQINGPWDEAK 325
           L+ +  V A+L+ +++  IS + P++ + L    K+ I+GPWDEA+
Sbjct: 243 LLSISAVGAILFALLLFSISCLCPYYLIHLQ-LFKENIHGPWDEAE 287
>dbj|BAA22866.1| (AB000360) PIGC [Homo sapiens]
          Length = 297

 Score =  356 bits (905), Expect = 4e-97
 Identities = 85/286 (29%), Positives = 148/286 (51%), Gaps = 12/286 (4%)

Query: 41  VWRKVLYTRQPYEDNYVDPQQFLQDLRQNVNVATYEYNKVVLDTFVVIQQFSFVVFYLFV 100
            W+KVLY RQP+ DNYVD ++FL++LR+N++   Y+Y  VV ++ VVIQQ   V  ++ +
Sbjct: 13  KWQKVLYERQPFPDNYVD-RRFLEELRKNIHARKYQYWAVVFESSVVIQQLCSVCVFVVI 71

Query: 101 FSMMLSGRVDECALMWVNATLFTLAFVFCIVVQRQQAVDRGEIPTPFTHYLMGLCRQGIP 160
           +  M  G +   A  W+  T    + +  ++      +D GE             +  + 
Sbjct: 72  WWYMDEGLL---APHWLLGTGLASSLIGYVLF---DLIDGGEGRKKSGQTRWADLKSALV 125

Query: 161 LVGVLILLSPVLQTLTVAYSNDTIVTLSSLSMFVHVLLTDYNYLNCYSERYQQNTAVNAA 220
            +      SPVL+TLT + S DTI  +S   +  H++  DY      +       ++N A
Sbjct: 126 FITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDY---GANAAIVSSTLSLNMA 182

Query: 221 TFGIILIASRIQRVFQSGALIMFGILCFTLSPIPRHNLKHVSLRAHVVLTFTICALAIYF 280
            F  + +ASR+ R   +  ++ F I  F L P+ +  LK  + R++V +T      A+  
Sbjct: 183 IFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAVGG 242

Query: 281 LMEV-PVFALLYCVVVVVISVVIPFFFVKLHGSMKDQINGPWDEAK 325
           L+ +  V A+L+ ++++ IS +  F+ ++L    K+ I+GPWDEA+
Sbjct: 243 LLSISAVGAVLFALLLMSISCLCSFYLIRLQ-LFKENIHGPWDEAE 287
>pir||T41379 probable phosphatidylinositol- glycan-class c protein - fission
           yeast (Schizosaccharomyces pombe)
 emb|CAA19108.1| (AL023592) phosphatidylinositol-glycan-class c [Schizosaccharomyces
           pombe]
          Length = 324

 Score =  331 bits (840), Expect = 2e-89
 Identities = 83/288 (28%), Positives = 144/288 (49%), Gaps = 11/288 (3%)

Query: 42  WRKVLYTRQPYEDNYVDPQQFLQDLRQNVNVATYEYNKVVLDTFVVIQQFSFVVFYLFVF 101
           W+KVL+ +Q Y DN++D + FL  L++NVN+   ++  +V D+  V Q  S VV +  VF
Sbjct: 38  WKKVLWRKQDYPDNFID-ESFLNGLQRNVNIQVTDFWSLVADSLPVSQHLSSVVIFASVF 96

Query: 102 SMMLSGRVDECALMWVNATLFTLAFVFCIVVQRQQAVDRGEIPTPFTHYLMGLCRQGIPL 161
             +   ++    + +V+      AF+    V R+   +R      F +Y MG+ +  I +
Sbjct: 97  VSIYRNQLSCALVGFVSNVSAVAAFILWDFVLRKPCNNR-----TFPNY-MGIVKSCILI 150

Query: 162 VGVLILLSPVLQTLTVAYSNDTIVTLSSLSMFVHVLLTDYNYLNCYSE-RYQQNTAVNAA 220
           V  L  LSP+L +LT + S D++  ++      +V   +Y         R   + + NAA
Sbjct: 151 VLTLAGLSPILMSLTKSTSPDSVWAIAVWLFLANVFFHEYTTETIRPHVRLHNSLSTNAA 210

Query: 221 TFGIILIASRIQRVFQSGALIMFGILCFTLSPIPRHNLKHVSLRAHVVLTFTICALAIYF 280
               +++ASR+++       I+F +  F L PI R  +   S  A +++T  +   A   
Sbjct: 211 LSASVVLASRLEKSINVFFFILFAVHWFALFPIFRKYIHVFSFYADMLMTLVLIISAYIA 270

Query: 281 LMEVP--VFALLYCVVVVVISVVIPFFFVKLHGSMKDQINGPWDEAKP 326
           L  V   V A ++  ++  IS + P +F+KL    K++I+GPWD A P
Sbjct: 271 LNAVASVVIAFVFLSLIFFISFICPIWFIKLQ-RFKNEIHGPWDIALP 317
>ref|NP_501645.1| (NM_069244) T20D3.8.p [Caenorhabditis elegans]
 pir||T25032 hypothetical protein T20D3.8 - Caenorhabditis elegans
 emb|CAA92490.2| (Z68220) cDNA EST yk416d12.3 comes from this gene~cDNA EST
           yk416d12.5 comes from this gene~cDNA EST yk586b10.3
           comes from this gene~cDNA EST yk586b10.5 comes from this
           gene [Caenorhabditis elegans]
          Length = 282

 Score =  296 bits (751), Expect = 4e-79
 Identities = 72/293 (24%), Positives = 123/293 (41%), Gaps = 24/293 (8%)

Query: 42  WRKVLYTRQPYEDNYV-DPQQFLQDLRQNVNVATYEYNKVVLDTFVVIQQFSFVVFYLFV 100
           W+K+LY +QP+ DNY     QFL++LR+NV+V  Y+Y   V      +     +  Y  +
Sbjct: 7   WQKILYRKQPFPDNYSGGDAQFLKELRKNVSVVHYDYKSAVFGCMNFLTHLDMITMYFVL 66

Query: 101 FSMMLSGRVDECALMWVNATLFTLAFVFCIVVQRQQAVDRGEIPTPFTHYLMGLCRQGIP 160
           F  +L        L  V +    L   FC  +    A  +    T FT ++         
Sbjct: 67  FLNILHSNWSINILYSVFSLTIVLYLFFCKFLIPNPANAKEHARTIFTLFIFA------- 119

Query: 161 LVGVLILLSPVLQTLTVAYSNDTIVTLSSLSMFVHVLLTDYNYLNCYSERYQQNTAVNAA 220
                   +PV++TLT + S DTI + S ++        DY      +      T+V+  
Sbjct: 120 -----YAFTPVIRTLTTSISTDTIYSTSIITAIFSCFFHDY---GVKAPVVSYPTSVSTG 171

Query: 221 TFGIILIASRIQRVFQSGALIMFGILCFTLSPIPRHNLKHVSLRAHVVLTFT-ICALAIY 279
               I + SR++    +  L++            R+ + HV    +  L+ T  C L+++
Sbjct: 172 LSSAIFLLSRLEGDTPTLLLLVVAFTLHAYGAEFRNRIFHV----YPCLSSTIFCFLSLF 227

Query: 280 FLMEVPVFALLYCVVVVVISVVIPFF---FVKLHGSMKDQINGPWDEAKPTNS 329
            +  +  F+L   +   ++ + I F     + L  + K  I+GPWDEA P  S
Sbjct: 228 SIYCISDFSLELSICFALLHIFILFICPLILILKQTGKCTIHGPWDEAVPLKS 280
>gb|AAF47758.1| (AE003477) CG12077 gene product [Drosophila melanogaster]
          Length = 256

 Score =  275 bits (697), Expect = 9e-73
 Identities = 72/283 (25%), Positives = 113/283 (39%), Gaps = 52/283 (18%)

Query: 42  WRKVLYTRQPYEDNYVDPQQFLQDLRQNVNVATYEYNKVVLDTFVVIQQFSFVVFYLFVF 101
           W K LY+ + Y DNY D   FL+DLR N++   Y + + +    V+  Q S +  +L ++
Sbjct: 12  WVKNLYSNREYPDNYTD-ASFLKDLRTNLHCRIYTFGEAIAGITVLNNQISCITGFLILY 70

Query: 102 SMMLSGRVDECALMWVNATLFTLAFVFCIVVQRQQAVDRGEIPTPFTHYLMGLCRQGIPL 161
            +MLS  V    ++  +  +  + ++         A+   +  T  T  L G        
Sbjct: 71  QLMLSDSVSPTTILVPSCGITGIGYLCYRGRSLSWALLGEDSKTLVTVVLFG-------- 122

Query: 162 VGVLILLSPVLQTLTVAYSNDTIVTLSSLSMFVHVLLTDYNYLNCYSERYQQNTAVNAAT 221
                L SP+L TLT A S DTI T++   +  +++   Y                    
Sbjct: 123 ----YLFSPMLHTLTQAISTDTIYTMTFFVLLGNLIFGHYGL------------------ 160

Query: 222 FGIILIASRIQRVFQSGALIMFGILCFTLSPIPRHNLKHVSLRAHVVLTFTICALAIYFL 281
                            A++    + F L PI      H      ++  F IC +A+Y +
Sbjct: 161 ---------------DVAMVSKAAVFFVLYPIMTEANWHAGF---MLPIFAICCMALYCI 202

Query: 282 MEVPVFALLYCVVVVVISVVIPFFFVKLHGSMKDQINGPWDEA 324
              PV   LY    + I+ V PF FV      K  I+GPWDEA
Sbjct: 203 SR-PVL-YLYASTTIFINFVCPFIFV-WQQQYKFNIHGPWDEA 242
>ref|NP_181043.1| (NM_129050) putative phosphatidylinositol-glycan synthase
           [Arabidopsis thaliana]
 pir||T00479 hypothetical protein F19I3.21 - Arabidopsis thaliana
 gb|AAC12837.1| (AC004238) putative phosphatidylinositol-glycan synthase
           [Arabidopsis thaliana]
          Length = 303

 Score =  253 bits (640), Expect = 5e-66
 Identities = 75/296 (25%), Positives = 150/296 (50%), Gaps = 20/296 (6%)

Query: 39  GRVWRKVLY--TRQPYEDNYVDPQQFLQDLRQNVNVATYEYNKVVLDTFVVIQQFSFVVF 96
           G  WRKV Y   +  Y+DNY D + FL+++  N NV   +  KV+ D+  + Q    V  
Sbjct: 12  GPKWRKVAYGGMQIGYDDNYTD-ESFLEEMVMNANVVRRDLLKVMKDSVSISQYLCIVAL 70

Query: 97  YLFVFSMMLSGRVDECALMWVNATLFTLAFVFCIVVQRQQAVDRGEIPTPFTHYLMGLCR 156
            + V+   L   +DE +L+ ++ +L    F+  ++ +     ++    +    YL+ +  
Sbjct: 71  VVLVWVHTLESSLDENSLLLLDLSLLASGFLILLLTE-----EKMLSLSLLLRYLVNISF 125

Query: 157 QGIPLVGVLILLSPVLQTLTVAYSNDTIVTLSSLSMFVHVLLTDYNYLNCYS------ER 210
                   L +L+P+ QTLT + S+D+I  ++   + +H+ L DY+     +        
Sbjct: 126 ----FTTGLYILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTPN 181

Query: 211 YQQNTAVNAATFGIILIASRIQRVFQSGALIMFGILCFTLSPIPRHNLKHVSLRAHVVLT 270
                +VNA+    + +ASR+       A+++F +  F  +P+  + +K  +   H++ +
Sbjct: 182 LTSCISVNASIVASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTYCIKKFNFGLHLLFS 241

Query: 271 FTICALAIYFLMEV-PVFALLYCVVVVVISVVIPFFFVKLHGSMKDQINGPWDEAK 325
           F +  L +Y +  +  +F L++  +V++++VV P++ +++    K +INGPWDEAK
Sbjct: 242 FALMGLTLYSIYALHRLFFLVFLSLVLLVNVVCPYWLIRMQ-EYKFEINGPWDEAK 296
>ref|NP_015249.1| (NC_001148) Required for synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi2p
           [Saccharomyces cerevisiae]
 sp|P46961|GPI2_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI2
 pir||S61111 GPI2 protein - yeast (Saccharomyces cerevisiae)
 dbj|BAA06128.1| (D29645) Gcr4p [Saccharomyces cerevisiae]
 gb|AAB68262.1| (U41849) Gpi2p [Saccharomyces cerevisiae]
          Length = 280

 Score =  246 bits (622), Expect = 6e-64
 Identities = 46/277 (16%), Positives = 108/277 (38%), Gaps = 26/277 (9%)

Query: 42  WRKVLYTRQPYEDNYVDPQQFLQDLRQNVNVATYEYNKVVLDTFVVIQQFSFVVFYLFVF 101
           W+++L+ +Q Y DNY DP  F+ +LR        E N+          +    + ++  +
Sbjct: 6   WKRLLWLKQEYPDNYTDP-SFI-ELRAR---QKAESNQKSDRKLSEAARAQIRLDFISFY 60

Query: 102 SMMLSGRVDECALMWVNATLF-----TLAFVFCIVVQRQQAVDRGEIPTPFTHYLMGLCR 156
             +L+         ++    F     T+   F  ++  +  VD                +
Sbjct: 61  QTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSS-------FMDVK 113

Query: 157 QGIPLVGVLILLSPVLQTLTVAYSNDTIVTLSSLSMFVHVLLTDYNYLNCYSERYQQNTA 216
             + +   ++ LSPVL++L+   ++D+I TLS      ++ +      +  S+    N +
Sbjct: 114 SSLIITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFV----ISSTKSKDKPSNLS 169

Query: 217 VNAATFGIILIASRIQRVFQSGALIMFGILCFTLSPIPRHNLKHVSLRAHVVLTFTICAL 276
            N     + +++SR+         ++  I    + P        V      ++ ++   +
Sbjct: 170 TNILVALVAVLSSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVPIISNIIVYSFLNV 229

Query: 277 AIYFLMEVPVFALLYCVVVVVISVVIPFFFVKLHGSM 313
           A+ ++  +    L++   V  I+V+ P +F+    + 
Sbjct: 230 ALGWIYML----LIFFASVFYITVL-PKWFIYWKINY 261
>gb|AAA79518.1| (U23788) Gpi2p [Saccharomyces cerevisiae]
          Length = 268

 Score =  229 bits (579), Expect = 6e-59
 Identities = 43/263 (16%), Positives = 102/263 (38%), Gaps = 21/263 (7%)

Query: 42  WRKVLYTRQPYEDNYVDPQQFLQDLRQNVNVATYEYNKVVLDTFVVIQQFSFVVFYLFVF 101
           W+++L+ +Q Y DNY DP  F+ +LR        E N+         ++    + ++  +
Sbjct: 6   WKRLLWLKQEYPDNYTDP-SFI-ELRAR---QKAESNQKSDRKLSEARRAQIRLDFISFY 60

Query: 102 SMMLSGRVDECALMWVNATLF-----TLAFVFCIVVQRQQAVDRGEIPTPFTHYLMGLCR 156
             +L+         ++    F     T+   F  ++  +  VD                +
Sbjct: 61  QTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSS-------FMDVK 113

Query: 157 QGIPLVGVLILLSPVLQTLTVAYSNDTIVTLSSLSMFVHVLLTDYNYLNCYSERYQQNTA 216
             + +   ++ LSPVL++L+   ++D+I TLS      ++ +      +  S+    N +
Sbjct: 114 SSLIITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFV----ISSTKSKDKPSNLS 169

Query: 217 VNAATFGIILIASRIQRVFQSGALIMFGILCFTLSPIPRHNLKHVSLRAHVVLTFTICAL 276
            N     + +++SR+         ++  I    + P        V      ++ ++   +
Sbjct: 170 TNILVALVAVLSSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVPIISNIIVYSFLNV 229

Query: 277 AIYFLMEVPVFALLYCVVVVVIS 299
           A+ ++  +    L Y ++   +S
Sbjct: 230 ALGWIYMLLFSLLQYFILQFYLS 252
>ref|NP_491651.1| (NM_059250) collagen [Caenorhabditis elegans]
 pir||T25835 hypothetical protein M01A12.1 - Caenorhabditis elegans
 gb|AAB42221.1| (U88166) similar to collagen; coded for by C. elegans cDNA
           yk160a5.5 [Caenorhabditis elegans]
          Length = 599

 Score = 40.8 bits (95), Expect = 0.033
 Identities = 15/85 (17%), Positives = 32/85 (37%), Gaps = 8/85 (9%)

Query: 233 RVFQSGA-----LIMFGILCFTLSPIPRHNLKHVSLRAHVVLT-FTICALAIYF--LMEV 284
                          F + C  +S    + +K + L  ++V++ F    ++  +  L   
Sbjct: 230 TRKHDFFSSIYTFFAFCVQCSAVSETDENYVKSMELYFYIVVSQFVGIRMSYIWRQLGSP 289

Query: 285 PVFALLYCVVVVVISVVIPFFFVKL 309
            V   L    V+ ++V+ P  F+ L
Sbjct: 290 TVAITLSVSTVIYVTVICPIVFIHL 314
>pir||S22041 hypothetical protein 873 - common sunflower
 emb|CAA44478.1| (X62592) ORF 873 [Helianthus annuus]
 emb|CAA37614.1| (X53537) orfH522 [Helianthus annuus]
          Length = 291

 Score = 35.8 bits (82), Expect = 1.0
 Identities = 15/74 (20%), Positives = 30/74 (40%), Gaps = 12/74 (16%)

Query: 248 FTLSPIPRHNLKHV----SLRAHVVLTFTICALAIYFLMEVPV-----FALLYCVVVVVI 298
           FT+ PI    L             +LT     + +  +  + V      + L+ V++++I
Sbjct: 13  FTILPIFTMLLGRFLGTGCPGISTLLTLFFLIVTLLLIYRMRVSKRKKSSFLFFVILILI 72

Query: 299 SVVIPF---FFVKL 309
            V+  F   FF+ +
Sbjct: 73  YVLCTFSRSFFIFM 86
>ref|NP_039884.1| (NC_001345) BBRF3 late reading frame [Human herpesvirus 4]
 sp|P03215|VGLM_EBV GLYCOPROTEIN M
 pir||QQBE35 BBRF3 protein - human herpesvirus 4 (strain B95-8)
 emb|CAA24825.1| (V01555) BBRF3 late reading frame [Human herpesvirus 4]
          Length = 405

 Score = 34.6 bits (79), Expect = 2.9
 Identities = 28/198 (14%), Positives = 54/198 (27%), Gaps = 23/198 (11%)

Query: 86  VVIQQFSFVVFYLFVFSMMLSGRVDECALMWVNATLFTLAFVFCIVVQRQQAVDRGEIPT 145
           V IQ  S+   +L  F   L                   A            V   +   
Sbjct: 140 VFIQVLSYKHVFLSAFVYFLH-----------FLASVLHACACVTRFSPVWVVKAQDNSI 188

Query: 146 PFTHYLMGLCRQGIPLVGVLILLSPVLQTLTVAYSNDTIVTLSSLSMFVHVLLTDYNYLN 205
           P   +L  +     P+V  L L    L+TL        + +LS      +        + 
Sbjct: 189 PQDTFLWWVVFYLKPVVTNLYLGCLALETL--------VFSLSVFLALGNSFYFMVGDMV 240

Query: 206 CYSERYQQNTAVNAATFGIILIASRIQRVFQSGALIMFGILCFTLSPIPRHNLKHVSLRA 265
             +        +       + +AS ++  F     +        + P+ R+        +
Sbjct: 241 LGAVNLFLILPIFWYILTEVWLASFLRHNFGFYCGMFIA-SIILILPLVRY---EAVFVS 296

Query: 266 HVVLTFTICALAIYFLME 283
             + T     +AI  ++ 
Sbjct: 297 AKLHTTVAINVAIIPILC 314
CPU time:   106.17 user secs.	    2.30 sys. secs	  108.47 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.317    0.169    0.486 

Gapped
Lambda     K      H
   0.270   0.0593    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 219789150
Number of Sequences: 887402
Number of extensions: 10847289
Number of successful extensions: 54886
Number of sequences better than 10.0: 565
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 569
Number of HSP's that attempted gapping in prelim test: 54044
Number of HSP's gapped (non-prelim): 1036
length of query: 342
length of database: 277,845,442
effective HSP length: 54
effective length of query: 288
effective length of database: 229,925,734
effective search space: 66218611392
effective search space used: 66218611392
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.8 bits)
S2: 74 (32.9 bits)