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GPI Anchor Biosynthesis Report: T41379 (PIG-C family, Schizosaccharomyces pombe)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (324 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 1

Distribution of 11 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)
Sequences producing significant alignments:
pir||T41379 probable phosphatidylinositol- glycan-class c protei... 434 e-120
dbj|BAB29152.1| (AK014096) data source:SPTR, source key:Q92535, ... 383 e-105
ref|NP_002633.1| (NM_002642) phosphatidylinositol glycan, class ... 380 e-104
dbj|BAA22866.1| (AB000360) PIGC [Homo sapiens] 376 e-103
ref|NP_501645.1| (NM_069244) T20D3.8.p [Caenorhabditis elegans] ... 305 7e-82
ref|NP_181043.1| (NM_129050) putative phosphatidylinositol-glyca... 267 2e-70
ref|NP_015249.1| (NC_001148) Required for synthesis of N-acetylg... 248 1e-64
gb|AAA79518.1| (U23788) Gpi2p [Saccharomyces cerevisiae] 228 1e-58
gb|AAF47758.1| (AE003477) CG12077 gene product [Drosophila melan... 206 4e-52
Sequences with E-value WORSE than threshold

gb|AAC06013.1| (AF051936) ryanodine receptor [Homarus americanus] 39 0.10
ref|NP_495657.1| (NM_063256) sodium/phosphate transporter [Caeno... 33 8.5
Alignments
>pir||T41379 probable phosphatidylinositol- glycan-class c protein - fission
           yeast (Schizosaccharomyces pombe)
 emb|CAA19108.1| (AL023592) phosphatidylinositol-glycan-class c [Schizosaccharomyces
           pombe]
          Length = 324

 Score =  434 bits (1105), Expect = e-120
 Identities = 324/324 (100%), Positives = 324/324 (100%)

Query: 1   MDEVCAAPAPKKMVAKSLVLNTFRKASVKEVVELKKPWKKVLWRKQDYPDNFIDESFLNG 60
           MDEVCAAPAPKKMVAKSLVLNTFRKASVKEVVELKKPWKKVLWRKQDYPDNFIDESFLNG
Sbjct: 1   MDEVCAAPAPKKMVAKSLVLNTFRKASVKEVVELKKPWKKVLWRKQDYPDNFIDESFLNG 60

Query: 61  LQRNVNIQVTDFWSLVADSLPVSQHLSSVVIFASVFVSIYRNQLSCALVGFVSNVSAVAA 120
           LQRNVNIQVTDFWSLVADSLPVSQHLSSVVIFASVFVSIYRNQLSCALVGFVSNVSAVAA
Sbjct: 61  LQRNVNIQVTDFWSLVADSLPVSQHLSSVVIFASVFVSIYRNQLSCALVGFVSNVSAVAA 120

Query: 121 FILWDFVLRKPCNNRTFPNYMGIVKSCILIVLTLAGLSPILMSLTKSTSPDSVWAIAVWL 180
           FILWDFVLRKPCNNRTFPNYMGIVKSCILIVLTLAGLSPILMSLTKSTSPDSVWAIAVWL
Sbjct: 121 FILWDFVLRKPCNNRTFPNYMGIVKSCILIVLTLAGLSPILMSLTKSTSPDSVWAIAVWL 180

Query: 181 FLANVFFHEYTTETIRPHVRLHNSLSTNAALSASVVLASRLEKSINVFFFILFAVHWFAL 240
           FLANVFFHEYTTETIRPHVRLHNSLSTNAALSASVVLASRLEKSINVFFFILFAVHWFAL
Sbjct: 181 FLANVFFHEYTTETIRPHVRLHNSLSTNAALSASVVLASRLEKSINVFFFILFAVHWFAL 240

Query: 241 FPIFRKYIHVFSFYADMLMTLVLIISAYIALNAVASVVIAFVFLSLIFFISFICPIWFIK 300
           FPIFRKYIHVFSFYADMLMTLVLIISAYIALNAVASVVIAFVFLSLIFFISFICPIWFIK
Sbjct: 241 FPIFRKYIHVFSFYADMLMTLVLIISAYIALNAVASVVIAFVFLSLIFFISFICPIWFIK 300

Query: 301 LQRFKNEIHGPWDIALPKLGPSKG 324
           LQRFKNEIHGPWDIALPKLGPSKG
Sbjct: 301 LQRFKNEIHGPWDIALPKLGPSKG 324
>dbj|BAB29152.1| (AK014096) data source:SPTR, source key:Q92535,
           evidence:ISS~homolog to PHOSPHATIDYLINOSITOL-GLYCAN
           BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)~putative [Mus
           musculus]
 gb|AAH06938.1|AAH06938 (BC006938) RIKEN cDNA 3110030E07 gene [Mus musculus]
          Length = 297

 Score =  383 bits (974), Expect = e-105
 Identities = 94/288 (32%), Positives = 167/288 (57%), Gaps = 7/288 (2%)

Query: 28  VKEVVELKKPWKKVLWRKQDYPDNFIDESFLNGLQRNVNIQVTDFWSLVADSLPVSQHLS 87
           V +  E+K  W+KVL+ +Q +PDN++D+ FL  L++N+  +   +W++V +S  V Q L 
Sbjct: 6   VTDTPEVK--WQKVLYERQPFPDNYVDQRFLEELRKNIYARKYQYWAVVFESSVVIQQLC 63

Query: 88  SVVIFASVFVSIYRNQLSCALVGFVSNVSAVAAFILWDFVLRKPCNNRTFPNYMGIVKSC 147
           SV +F  ++  +    L+   +      S++  ++L+D +       ++       +KS 
Sbjct: 64  SVCVFVVIWWYMDEGLLAPQWLFGTGLASSLVGYVLFDLIDGGDGRKKSGRTRWADLKST 123

Query: 148 ILIVLTLAGLSPILMSLTKSTSPDSVWAIAVWLFLANVFFHEYTTETIRPHVRLHNSLST 207
           ++ +    G SP+L +LT+S S D+++A+AV++ L ++ F +Y          + ++LS 
Sbjct: 124 LVFITFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANA----AIVSSTLSL 179

Query: 208 NAALSASVVLASRLEKSINVFFFILFAVHWFALFPIFRKYIHVFSFYADMLMTLVLIISA 267
           N A+ ASV LASRL +S++ F  + FA+  FAL+P+ +K +  ++  + + +TL+   SA
Sbjct: 180 NMAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAFSA 239

Query: 268 YIALNAVASVVIAFVFLSLIFFISFICPIWFIKLQRFKNEIHGPWDIA 315
           +  L ++ S V A +F  L+F IS +CP + I LQ FK  IHGPWD A
Sbjct: 240 FGGLLSI-SAVGAILFALLLFSISCLCPYYLIHLQLFKENIHGPWDEA 286
>ref|NP_002633.1| (NM_002642) phosphatidylinositol glycan, class C [Homo sapiens]
 ref|XP_002139.1| (XM_002139) phosphatidylinositol glycan, class C [Homo sapiens]
 sp|Q92535|PIGC_HUMAN PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)
 pir||JC4969 pig-c protein - human
 dbj|BAA12812.1| (D85418) phosphatidylinositol-glycan-class C (PIG-C) [Homo sapiens]
 emb|CAB10071.1| (Z97195) PHOSPHATIDYLINISITOL-GLYCAN class C (PIG-C) [Homo sapiens]
 gb|AAH06539.1|AAH06539 (BC006539) phosphatidylinositol glycan, class C [Homo sapiens]
          Length = 297

 Score =  380 bits (968), Expect = e-104
 Identities = 92/288 (31%), Positives = 164/288 (56%), Gaps = 7/288 (2%)

Query: 28  VKEVVELKKPWKKVLWRKQDYPDNFIDESFLNGLQRNVNIQVTDFWSLVADSLPVSQHLS 87
           V    E+K  W+KVL+ +Q +PDN++D  FL  L++N++ +   +W++V +S  V Q L 
Sbjct: 6   VTNTKEVK--WQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQQLC 63

Query: 88  SVVIFASVFVSIYRNQLSCALVGFVSNVSAVAAFILWDFVLRKPCNNRTFPNYMGIVKSC 147
           SV +F  ++  +    L+   +      S++  ++L+D +       ++       +KS 
Sbjct: 64  SVCVFVVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADLKSA 123

Query: 148 ILIVLTLAGLSPILMSLTKSTSPDSVWAIAVWLFLANVFFHEYTTETIRPHVRLHNSLST 207
           ++ +    G SP+L +LT+S S D+++A++V++ L ++ F +Y          + ++LS 
Sbjct: 124 LVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANA----AIVSSTLSL 179

Query: 208 NAALSASVVLASRLEKSINVFFFILFAVHWFALFPIFRKYIHVFSFYADMLMTLVLIISA 267
           N A+ ASV LASRL +S++ F  + FA+  FAL+P+ +K +   +  + + +TL+   SA
Sbjct: 180 NMAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSA 239

Query: 268 YIALNAVASVVIAFVFLSLIFFISFICPIWFIKLQRFKNEIHGPWDIA 315
              L ++ S V A +F  L+  IS +CP + I+LQ FK  IHGPWD A
Sbjct: 240 VGGLLSI-SAVGAVLFALLLMSISCLCPFYLIRLQLFKENIHGPWDEA 286
>dbj|BAA22866.1| (AB000360) PIGC [Homo sapiens]
          Length = 297

 Score =  376 bits (957), Expect = e-103
 Identities = 91/288 (31%), Positives = 163/288 (56%), Gaps = 7/288 (2%)

Query: 28  VKEVVELKKPWKKVLWRKQDYPDNFIDESFLNGLQRNVNIQVTDFWSLVADSLPVSQHLS 87
           V    E+K  W+KVL+ +Q +PDN++D  FL  L++N++ +   +W++V +S  V Q L 
Sbjct: 6   VTNTKEVK--WQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQQLC 63

Query: 88  SVVIFASVFVSIYRNQLSCALVGFVSNVSAVAAFILWDFVLRKPCNNRTFPNYMGIVKSC 147
           SV +F  ++  +    L+   +      S++  ++L+D +       ++       +KS 
Sbjct: 64  SVCVFVVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADLKSA 123

Query: 148 ILIVLTLAGLSPILMSLTKSTSPDSVWAIAVWLFLANVFFHEYTTETIRPHVRLHNSLST 207
           ++ +    G SP+L +LT+S S D+++A++V++ L ++ F +Y          + ++LS 
Sbjct: 124 LVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANA----AIVSSTLSL 179

Query: 208 NAALSASVVLASRLEKSINVFFFILFAVHWFALFPIFRKYIHVFSFYADMLMTLVLIISA 267
           N A+ ASV LASRL +S++ F  + FA+  FAL+P+ +K +   +  + + +TL+   SA
Sbjct: 180 NMAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSA 239

Query: 268 YIALNAVASVVIAFVFLSLIFFISFICPIWFIKLQRFKNEIHGPWDIA 315
              L ++ S V A +F  L+  IS +C  + I+LQ FK  IHGPWD A
Sbjct: 240 VGGLLSI-SAVGAVLFALLLMSISCLCSFYLIRLQLFKENIHGPWDEA 286
>ref|NP_501645.1| (NM_069244) T20D3.8.p [Caenorhabditis elegans]
 pir||T25032 hypothetical protein T20D3.8 - Caenorhabditis elegans
 emb|CAA92490.2| (Z68220) cDNA EST yk416d12.3 comes from this gene~cDNA EST
           yk416d12.5 comes from this gene~cDNA EST yk586b10.3
           comes from this gene~cDNA EST yk586b10.5 comes from this
           gene [Caenorhabditis elegans]
          Length = 282

 Score =  305 bits (774), Expect = 7e-82
 Identities = 79/283 (27%), Positives = 139/283 (48%), Gaps = 15/283 (5%)

Query: 38  WKKVLWRKQDYPDNFI--DESFLNGLQRNVNIQVTDFWSLVADSLPVSQHLSSVVIFASV 95
           W+K+L+RKQ +PDN+   D  FL  L++NV++   D+ S V   +    HL  + ++  +
Sbjct: 7   WQKILYRKQPFPDNYSGGDAQFLKELRKNVSVVHYDYKSAVFGCMNFLTHLDMITMYFVL 66

Query: 96  FVSIYRNQLSCALVGFVSNVSAVAAFILWDFVLRKPCNNRTFPNYMGIVKSCILIVLTLA 155
           F++I  +  S  ++  V +++ V       F++  P N +         ++   + +   
Sbjct: 67  FLNILHSNWSINILYSVFSLTIVLYLFFCKFLIPNPANAKEH------ARTIFTLFIFAY 120

Query: 156 GLSPILMSLTKSTSPDSVWAIAVWLFLANVFFHEYTTETIRPHVRLHNSLSTNAALSASV 215
             +P++ +LT S S D++++ ++   + + FFH+Y  +   P V    S+ST   LS+++
Sbjct: 121 AFTPVIRTLTTSISTDTIYSTSIITAIFSCFFHDYGVKA--PVVSYPTSVST--GLSSAI 176

Query: 216 VLASRLEKSINVFFFILFAVHWFALFPIFRKYI-HVFSFYADMLMTLVLIISAYIALNAV 274
            L SRLE        ++ A    A    FR  I HV+   +  +   + + S Y   +  
Sbjct: 177 FLLSRLEGDTPTLLLLVVAFTLHAYGAEFRNRIFHVYPCLSSTIFCFLSLFSIYCISDFS 236

Query: 275 ASVVIAFVFLSLIFFISFICPIWFIKLQRFKNEIHGPWDIALP 317
             + I F  L +  FI FICP+  I  Q  K  IHGPWD A+P
Sbjct: 237 LELSICFALLHI--FILFICPLILILKQTGKCTIHGPWDEAVP 277
>ref|NP_181043.1| (NM_129050) putative phosphatidylinositol-glycan synthase
           [Arabidopsis thaliana]
 pir||T00479 hypothetical protein F19I3.21 - Arabidopsis thaliana
 gb|AAC12837.1| (AC004238) putative phosphatidylinositol-glycan synthase
           [Arabidopsis thaliana]
          Length = 303

 Score =  267 bits (676), Expect = 2e-70
 Identities = 88/285 (30%), Positives = 146/285 (50%), Gaps = 11/285 (3%)

Query: 38  WKKVLW--RKQDYPDNFIDESFLNGLQRNVNIQVTDFWSLVADSLPVSQHLSSVVIFASV 95
           W+KV +   +  Y DN+ DESFL  +  N N+   D   ++ DS+ +SQ+L  V +   V
Sbjct: 15  WRKVAYGGMQIGYDDNYTDESFLEEMVMNANVVRRDLLKVMKDSVSISQYLCIVALVVLV 74

Query: 96  FVSIYRNQLSCALVGFVSNVSAVAAFILWDFVLRKPCNNRTFPNYMGIVKSCILIVLTLA 155
           +V    + L    +  +      + F++      K  +      Y+  +         L 
Sbjct: 75  WVHTLESSLDENSLLLLDLSLLASGFLILLLTEEKMLSLSLLLRYLVNIS---FFTTGLY 131

Query: 156 GLSPILMSLTKSTSPDSVWAIAVWLFLANVFFHEYTTETIRP-----HVRLHNSLSTNAA 210
            L+PI  +LT+S S DS+WA+ V L L ++F H+Y+  TIR         L + +S NA+
Sbjct: 132 ILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTPNLTSCISVNAS 191

Query: 211 LSASVVLASRLEKSINVFFFILFAVHWFALFPIFRKYIHVFSFYADMLMTLVLIISAYIA 270
           + ASV +ASRL   ++VF  +LF++  F   P+    I  F+F   +L +  L+     +
Sbjct: 192 IVASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTYCIKKFNFGLHLLFSFALMGLTLYS 251

Query: 271 LNAVASVVIAFVFLSLIFFISFICPIWFIKLQRFKNEIHGPWDIA 315
           + A+   +   VFLSL+  ++ +CP W I++Q +K EI+GPWD A
Sbjct: 252 IYALHR-LFFLVFLSLVLLVNVVCPYWLIRMQEYKFEINGPWDEA 295
>ref|NP_015249.1| (NC_001148) Required for synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi2p
           [Saccharomyces cerevisiae]
 sp|P46961|GPI2_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI2
 pir||S61111 GPI2 protein - yeast (Saccharomyces cerevisiae)
 dbj|BAA06128.1| (D29645) Gcr4p [Saccharomyces cerevisiae]
 gb|AAB68262.1| (U41849) Gpi2p [Saccharomyces cerevisiae]
          Length = 280

 Score =  248 bits (627), Expect = 1e-64
 Identities = 85/304 (27%), Positives = 136/304 (43%), Gaps = 46/304 (15%)

Query: 35  KKPWKKVLWRKQDYPDNFIDESFLNGLQRNVNIQVTDFWSLVADSLPVSQHLSSVVIFAS 94
           + PWK++LW KQ+YPDN+ D SF+    R             A+S   S    S    A 
Sbjct: 3   RSPWKRLLWLKQEYPDNYTDPSFIELRARQ-----------KAESNQKSDRKLSEAARAQ 51

Query: 95  V---FVSIYRNQLSCALVGFVSNVSAVAAF------ILWDFVLRKPCNNRTFPNYMGI-- 143
           +   F+S Y+  L+ + +           F      I   F+       +  P       
Sbjct: 52  IRLDFISFYQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMD 111

Query: 144 VKSCILIVLTLAGLSPILMSLTKSTSPDSVWAIAVWLFLANVFFHEYTTETIRPHVRLHN 203
           VKS ++I   +  LSP+L SL+K+T+ DS+W ++ WL L  +F    T    +P     +
Sbjct: 112 VKSSLIITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSKDKP-----S 166

Query: 204 SLSTNAALSASVVLASRLEKSINVFFFILFAVHWFALFPIFRKYIHVFSFYADMLMTLVL 263
           +LSTN  ++   VL+SRL  +I+VF F+L  +    + P           Y  +   +V 
Sbjct: 167 NLSTNILVALVAVLSSRLSTTIDVFCFLLICIQLNIILPT----------YLSVTNKVVP 216

Query: 264 IISAYIALNAVASVVIAFVFLSLIFFISF----ICPIWF----IKLQRFKNEIHGPWDIA 315
           IIS  I  + + +V + ++++ LIFF S     + P WF    I   +  N++   WD  
Sbjct: 217 IISNIIVYSFL-NVALGWIYMLLIFFASVFYITVLPKWFIYWKINYHKRDNDLLSTWDAR 275

Query: 316 LPKL 319
            P L
Sbjct: 276 TPIL 279
>gb|AAA79518.1| (U23788) Gpi2p [Saccharomyces cerevisiae]
          Length = 268

 Score =  228 bits (576), Expect = 1e-58
 Identities = 72/267 (26%), Positives = 118/267 (43%), Gaps = 34/267 (12%)

Query: 35  KKPWKKVLWRKQDYPDNFIDESFLNGLQRNVNIQVTDFWSLVADSLPVSQHLSSVVIFAS 94
           + PWK++LW KQ+YPDN+ D SF+    R             A+S   S    S    A 
Sbjct: 3   RSPWKRLLWLKQEYPDNYTDPSFIELRARQ-----------KAESNQKSDRKLSEARRAQ 51

Query: 95  V---FVSIYRNQLSCALVGFVSNVSAVAAF------ILWDFVLRKPCNNRTFPNYMGI-- 143
           +   F+S Y+  L+ + +           F      I   F+       +  P       
Sbjct: 52  IRLDFISFYQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMD 111

Query: 144 VKSCILIVLTLAGLSPILMSLTKSTSPDSVWAIAVWLFLANVFFHEYTTETIRPHVRLHN 203
           VKS ++I   +  LSP+L SL+K+T+ DS+W ++ WL L  +F    T    +P     +
Sbjct: 112 VKSSLIITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSKDKP-----S 166

Query: 204 SLSTNAALSASVVLASRLEKSINVFFFILFAVHWFALFPIFRKYIHVFSFYADMLMTLVL 263
           +LSTN  ++   VL+SRL  +I+VF F+L  +    + P +           + ++ ++ 
Sbjct: 167 NLSTNILVALVAVLSSRLSTTIDVFCFLLICIQLNIILPTYLS-------VTNKVVPIIS 219

Query: 264 IISAYIALNAVASVVIAFVFLSLIFFI 290
            I  Y  LN     +   +F  L +FI
Sbjct: 220 NIIVYSFLNVALGWIYMLLFSLLQYFI 246
>gb|AAF47758.1| (AE003477) CG12077 gene product [Drosophila melanogaster]
          Length = 256

 Score =  206 bits (521), Expect = 4e-52
 Identities = 64/289 (22%), Positives = 131/289 (45%), Gaps = 47/289 (16%)

Query: 28  VKEVVELKKPWKKVLWRKQDYPDNFIDESFLNGLQRNVNIQVTDFWSLVADSLPVSQHLS 87
           VK     ++PW K L+  ++YPDN+ D SFL  L+ N++ ++  F   +A    ++  +S
Sbjct: 2   VKNGQSKRQPWVKNLYSNREYPDNYTDASFLKDLRTNLHCRIYTFGEAIAGITVLNNQIS 61

Query: 88  SVVIFASVFVSIYRNQLSCALVGFVSNVSAVAAFILWDFVLRKPCNNRTFPNYMGIVKSC 147
            +  F  ++  +  + +S   +   S       ++ +         + ++       K+ 
Sbjct: 62  CITGFLILYQLMLSDSVSPTTILVPSCGITGIGYLCYR------GRSLSWALLGEDSKTL 115

Query: 148 ILIVLTLAGLSPILMSLTKSTSPDSVWAIAVWLFLANVFFHEYTTETIRPHVRLHNSLST 207
           + +VL     SP+L +LT++ S D+++ +  ++ L N+ F  Y  +              
Sbjct: 116 VTVVLFGYLFSPMLHTLTQAISTDTIYTMTFFVLLGNLIFGHYGLD-------------- 161

Query: 208 NAALSASVVLASRLEKSINVFFFILFAVHWFALFPIFRKYIHVFSFYADMLMTLVLIISA 267
                  V + S+             A  +F L+PI  +     +++A  ++ +  I   
Sbjct: 162 -------VAMVSK-------------AAVFFVLYPIMTEA----NWHAGFMLPIFAICC- 196

Query: 268 YIALNAVASVVIAFVFLSLIFFISFICPIWFIKLQRFKNEIHGPWDIAL 316
            +AL  ++  V+ +++ S   FI+F+CP  F+  Q++K  IHGPWD A+
Sbjct: 197 -MALYCISRPVL-YLYASTTIFINFVCPFIFVWQQQYKFNIHGPWDEAI 243
>gb|AAC06013.1| (AF051936) ryanodine receptor [Homarus americanus]
          Length = 1513

 Score = 39.3 bits (91), Expect = 0.10
 Identities = 25/131 (19%), Positives = 52/131 (39%), Gaps = 18/131 (13%)

Query: 11   KKMVAKSLVLNTFRKASVKEVVELKKPWKKVLWRKQDYPDNFIDESFLNGLQRNVNIQVT 70
            +K VA+ L  +    A   E  ++K  W K++   + +P+N+ D  F+    R    +  
Sbjct: 1151 EKDVARRLEFDGVYVAEQPEDDDIKAHWDKLVISAKSFPNNYWD-KFVKKKVRQKYSETY 1209

Query: 71   DFWSL-----VADSLPVSQ---------HLSSVVIFASVF---VSIYRNQLSCALVGFVS 113
            DF ++     +  ++   Q         +++SV     V+   V++  NQ    L     
Sbjct: 1210 DFDAISNLLGMETTMSFKQEEASTGILGYITSVDWRYQVWKAGVTVTDNQFLYNLWYLTF 1269

Query: 114  NVSAVAAFILW 124
            +V     +  +
Sbjct: 1270 SVLGNINYFFF 1280
>ref|NP_495657.1| (NM_063256) sodium/phosphate transporter [Caenorhabditis elegans]
 pir||T24333 hypothetical protein T01H3.3 - Caenorhabditis elegans
 emb|CAA92687.1| (Z68317) similar to sodium/phosphate transporter~cDNA EST
           EMBL:T01627 comes from this gene~cDNA EST yk181g5.5
           comes from this gene~cDNA EST yk181g5.3 comes from this
           gene~cDNA EST yk240h2.5 comes from this gene
           [Caenorhabditis elegans]
          Length = 599

 Score = 32.6 bits (74), Expect = 8.5
 Identities = 35/246 (14%), Positives = 77/246 (31%), Gaps = 48/246 (19%)

Query: 75  LVADSLPVSQHLSSVVIFASVFVSIYRNQLSCALVGFVSNVSAVAAFILWDFV--LRKPC 132
           ++  S      +    +     + + ++     ++  +       ++I       L    
Sbjct: 210 VIFGSFVTINLIKKFGVRKVFSICMVQS----TILILLFLYVQKRSYIFLLLTRFLLGFS 265

Query: 133 NNRTFPNY------MGIVKSCILIVLTLAGL---SPIL--------MSLTKSTSPDSVW- 174
            +  FP         G +K  ++ + ++       P++         +L    S DSV+ 
Sbjct: 266 FSSVFPVMGNLLIQWGPLKEQLIFITSMFMFLSIGPVVSWPIAHYFHAL--EISLDSVYY 323

Query: 175 ----AIAVWLFLANVFFHE--------YTTETIRPHVRLHNSLSTNAALSASVVLASRLE 222
                +A    + ++F+ +           E  +      N L +N AL+ +     R  
Sbjct: 324 IQCGTLAFLTLIWSIFYRDRPQDHPWVSGVELNKIVAGKVNELRSNRALADAYTSLFRSL 383

Query: 223 K--SINVFFFILFAVHWF--ALFPIFRKYIHVF-----SFYADMLMTLVLI-ISAYIALN 272
              SI    F LF+V  F     P       VF       ++     L+ +    + +LN
Sbjct: 384 SAWSIWTSAFGLFSVTAFFSIYLPSLLASPDVFVVEGLGIHSSFPFQLLPLSCIIFSSLN 443

Query: 273 AVASVV 278
            +    
Sbjct: 444 VLFKPS 449
CPU time:    99.42 user secs.	    2.30 sys. secs	  101.72 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.318    0.170    0.490 

Gapped
Lambda     K      H
   0.270   0.0595    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 195613734
Number of Sequences: 887402
Number of extensions: 8828939
Number of successful extensions: 52424
Number of sequences better than 10.0: 552
Number of HSP's better than 10.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 552
Number of HSP's that attempted gapping in prelim test: 51730
Number of HSP's gapped (non-prelim): 1018
length of query: 324
length of database: 277,845,442
effective HSP length: 52
effective length of query: 272
effective length of database: 231,700,538
effective search space: 63022546336
effective search space used: 63022546336
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.9 bits)
S2: 74 (32.9 bits)