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GPI Anchor Biosynthesis Report: PIGC_HUMAN (PIG-C family, Homo sapiens)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (297 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 2

Distribution of 11 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)
Sequences producing significant alignments:
ref|NP_002633.1| (NM_002642) phosphatidylinositol glycan, class ... 447 e-124
dbj|BAA22866.1| (AB000360) PIGC [Homo sapiens] 443 e-123
dbj|BAB29152.1| (AK014096) data source:SPTR, source key:Q92535, ... 436 e-121
pir||T41379 probable phosphatidylinositol- glycan-class c protei... 343 3e-93
gb|AAF47758.1| (AE003477) CG12077 gene product [Drosophila melan... 307 2e-82
ref|NP_501645.1| (NM_069244) T20D3.8.p [Caenorhabditis elegans] ... 296 3e-79
ref|NP_181043.1| (NM_129050) putative phosphatidylinositol-glyca... 253 4e-66
ref|NP_015249.1| (NC_001148) Required for synthesis of N-acetylg... 231 9e-60
gb|AAA79518.1| (U23788) Gpi2p [Saccharomyces cerevisiae] 226 3e-58
Sequences with E-value WORSE than threshold

gb|AAD54698.1| (AF118772) ORF5 [Equine arteritis virus] 33 8.4
Alignments
>ref|NP_002633.1| (NM_002642) phosphatidylinositol glycan, class C [Homo sapiens]
 ref|XP_002139.1| (XM_002139) phosphatidylinositol glycan, class C [Homo sapiens]
 sp|Q92535|PIGC_HUMAN PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)
 pir||JC4969 pig-c protein - human
 dbj|BAA12812.1| (D85418) phosphatidylinositol-glycan-class C (PIG-C) [Homo sapiens]
 emb|CAB10071.1| (Z97195) PHOSPHATIDYLINISITOL-GLYCAN class C (PIG-C) [Homo sapiens]
 gb|AAH06539.1|AAH06539 (BC006539) phosphatidylinositol glycan, class C [Homo sapiens]
          Length = 297

 Score =  447 bits (1139), Expect = e-124
 Identities = 297/297 (100%), Positives = 297/297 (100%)

Query: 1   MYAQPVTNTKEVKWQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQ 60
           MYAQPVTNTKEVKWQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQ
Sbjct: 1   MYAQPVTNTKEVKWQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQ 60

Query: 61  QLCSVCVFVVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADL 120
           QLCSVCVFVVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADL
Sbjct: 61  QLCSVCVFVVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADL 120

Query: 121 KSALVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLN 180
           KSALVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLN
Sbjct: 121 KSALVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLN 180

Query: 181 MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAV 240
           MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAV
Sbjct: 181 MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAV 240

Query: 241 GGLLSISAVGAVLFALLLMSISCLCPFYLIRLQLFKENIHGPWDEAEIKEDLSRFLS 297
           GGLLSISAVGAVLFALLLMSISCLCPFYLIRLQLFKENIHGPWDEAEIKEDLSRFLS
Sbjct: 241 GGLLSISAVGAVLFALLLMSISCLCPFYLIRLQLFKENIHGPWDEAEIKEDLSRFLS 297
>dbj|BAA22866.1| (AB000360) PIGC [Homo sapiens]
          Length = 297

 Score =  443 bits (1129), Expect = e-123
 Identities = 296/297 (99%), Positives = 296/297 (99%)

Query: 1   MYAQPVTNTKEVKWQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQ 60
           MYAQPVTNTKEVKWQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQ
Sbjct: 1   MYAQPVTNTKEVKWQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQ 60

Query: 61  QLCSVCVFVVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADL 120
           QLCSVCVFVVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADL
Sbjct: 61  QLCSVCVFVVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADL 120

Query: 121 KSALVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLN 180
           KSALVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLN
Sbjct: 121 KSALVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLN 180

Query: 181 MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAV 240
           MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAV
Sbjct: 181 MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAV 240

Query: 241 GGLLSISAVGAVLFALLLMSISCLCPFYLIRLQLFKENIHGPWDEAEIKEDLSRFLS 297
           GGLLSISAVGAVLFALLLMSISCLC FYLIRLQLFKENIHGPWDEAEIKEDLSRFLS
Sbjct: 241 GGLLSISAVGAVLFALLLMSISCLCSFYLIRLQLFKENIHGPWDEAEIKEDLSRFLS 297
>dbj|BAB29152.1| (AK014096) data source:SPTR, source key:Q92535,
           evidence:ISS~homolog to PHOSPHATIDYLINOSITOL-GLYCAN
           BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)~putative [Mus
           musculus]
 gb|AAH06938.1|AAH06938 (BC006938) RIKEN cDNA 3110030E07 gene [Mus musculus]
          Length = 297

 Score =  436 bits (1110), Expect = e-121
 Identities = 278/297 (93%), Positives = 287/297 (96%)

Query: 1   MYAQPVTNTKEVKWQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQ 60
           M AQ VT+T EVKWQKVLYERQPFPDNYVD+RFLEELRKNI+ARKYQYWAVVFESSVVIQ
Sbjct: 1   MCAQRVTDTPEVKWQKVLYERQPFPDNYVDQRFLEELRKNIYARKYQYWAVVFESSVVIQ 60

Query: 61  QLCSVCVFVVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADL 120
           QLCSVCVFVVIWWYMDEGLLAP WL GTGLASSL+GYVLFDLIDGG+GRKKSG+TRWADL
Sbjct: 61  QLCSVCVFVVIWWYMDEGLLAPQWLFGTGLASSLVGYVLFDLIDGGDGRKKSGRTRWADL 120

Query: 121 KSALVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLN 180
           KS LVFITFTYGFSPVLKTLTESVSTDTIYAM+VFMLLGHLIFFDYGANAAIVSSTLSLN
Sbjct: 121 KSTLVFITFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANAAIVSSTLSLN 180

Query: 181 MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAV 240
           MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKA TPRSYVGVTLLFAFSA 
Sbjct: 181 MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAFSAF 240

Query: 241 GGLLSISAVGAVLFALLLMSISCLCPFYLIRLQLFKENIHGPWDEAEIKEDLSRFLS 297
           GGLLSISAVGA+LFALLL SISCLCP+YLI LQLFKENIHGPWDEAEIKEDLSRFLS
Sbjct: 241 GGLLSISAVGAILFALLLFSISCLCPYYLIHLQLFKENIHGPWDEAEIKEDLSRFLS 297
>pir||T41379 probable phosphatidylinositol- glycan-class c protein - fission
           yeast (Schizosaccharomyces pombe)
 emb|CAA19108.1| (AL023592) phosphatidylinositol-glycan-class c [Schizosaccharomyces
           pombe]
          Length = 324

 Score =  343 bits (871), Expect = 3e-93
 Identities = 89/281 (31%), Positives = 161/281 (56%), Gaps = 5/281 (1%)

Query: 11  EVKWQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQQLCSVCVFVV 70
           +  W+KVL+ +Q +PDN++D  FL  L++N++ +   +W++V +S  V Q L SV +F  
Sbjct: 35  KKPWKKVLWRKQDYPDNFIDESFLNGLQRNVNIQVTDFWSLVADSLPVSQHLSSVVIFAS 94

Query: 71  IWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADLKSALVFITFT 130
           ++  +    L+   +      S++  ++L+D +       ++       +KS ++ +   
Sbjct: 95  VFVSIYRNQLSCALVGFVSNVSAVAAFILWDFVLRKPCNNRTFPNYMGIVKSCILIVLTL 154

Query: 131 YGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANA----AIVSSTLSLNMAIFAS 186
            G SP+L +LT+S S D+++A++V++ L ++ F +Y          + ++LS N A+ AS
Sbjct: 155 AGLSPILMSLTKSTSPDSVWAIAVWLFLANVFFHEYTTETIRPHVRLHNSLSTNAALSAS 214

Query: 187 VCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAVGGLLSI 246
           V LASRL +S++ F  + FA+  FAL+P+ +K +   +  + + +TL+   SA   L ++
Sbjct: 215 VVLASRLEKSINVFFFILFAVHWFALFPIFRKYIHVFSFYADMLMTLVLIISAYIALNAV 274

Query: 247 -SAVGAVLFALLLMSISCLCPFYLIRLQLFKENIHGPWDEA 286
            S V A +F  L+  IS +CP + I+LQ FK  IHGPWD A
Sbjct: 275 ASVVIAFVFLSLIFFISFICPIWFIKLQRFKNEIHGPWDIA 315
>gb|AAF47758.1| (AE003477) CG12077 gene product [Drosophila melanogaster]
          Length = 256

 Score =  307 bits (779), Expect = 2e-82
 Identities = 88/283 (31%), Positives = 139/283 (49%), Gaps = 42/283 (14%)

Query: 9   TKEVKWQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQQLCSVCVF 68
           +K   W K LY  + +PDNY D  FL++LR N+H R Y +   +   +V+  Q+  +  F
Sbjct: 7   SKRQPWVKNLYSNREYPDNYTDASFLKDLRTNLHCRIYTFGEAIAGITVLNNQISCITGF 66

Query: 69  VVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADLKSALVFIT 128
           ++++  M    ++P  +L      + IGY+ +       GR  S      D K+ +  + 
Sbjct: 67  LILYQLMLSDSVSPTTILVPSCGITGIGYLCY------RGRSLSWALLGEDSKTLVTVVL 120

Query: 129 FTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLNMAIFASVC 188
           F Y FSP+L TLT+++STDTIY M+ F+LLG+LIF  YG + A+VS          A+V 
Sbjct: 121 FGYLFSPMLHTLTQAISTDTIYTMTFFVLLGNLIFGHYGLDVAMVSK---------AAVF 171

Query: 189 LASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAVGGLLSISA 248
                                F L+P++ +            +  +FA   +  L  IS 
Sbjct: 172 ---------------------FVLYPIMTEANWHAGF-----MLPIFAICCM-ALYCISR 204

Query: 249 VGAVLFALLLMSISCLCPFYLIRLQLFKENIHGPWDEAEIKED 291
               L+A   + I+ +CPF  +  Q +K NIHGPWDEA ++E+
Sbjct: 205 PVLYLYASTTIFINFVCPFIFVWQQQYKFNIHGPWDEAIVEEN 247
>ref|NP_501645.1| (NM_069244) T20D3.8.p [Caenorhabditis elegans]
 pir||T25032 hypothetical protein T20D3.8 - Caenorhabditis elegans
 emb|CAA92490.2| (Z68220) cDNA EST yk416d12.3 comes from this gene~cDNA EST
           yk416d12.5 comes from this gene~cDNA EST yk586b10.3
           comes from this gene~cDNA EST yk586b10.5 comes from this
           gene [Caenorhabditis elegans]
          Length = 282

 Score =  296 bits (752), Expect = 3e-79
 Identities = 86/280 (30%), Positives = 139/280 (48%), Gaps = 18/280 (6%)

Query: 14  WQKVLYERQPFPDNYV--DRRFLEELRKNIHARKYQYWAVVFESSVVIQQLCSVCVFVVI 71
           WQK+LY +QPFPDNY   D +FL+ELRKN+    Y Y + VF     +  L  + ++ V+
Sbjct: 7   WQKILYRKQPFPDNYSGGDAQFLKELRKNVSVVHYDYKSAVFGCMNFLTHLDMITMYFVL 66

Query: 72  WWYMDEGLLAPHW---LLGTGLASSLIGYVLF--DLIDGGEGRKKSGQTRWADLKSALVF 126
           +      +L  +W   +L +  + +++ Y+ F   LI      K+  +T           
Sbjct: 67  FL----NILHSNWSINILYSVFSLTIVLYLFFCKFLIPNPANAKEHART-------IFTL 115

Query: 127 ITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLNMAIFAS 186
             F Y F+PV++TLT S+STDTIY+ S+   +    F DYG  A +VS   S++  + ++
Sbjct: 116 FIFAYAFTPVIRTLTTSISTDTIYSTSIITAIFSCFFHDYGVKAPVVSYPTSVSTGLSSA 175

Query: 187 VCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAVGGLLSI 246
           + L SRL       +++  A  + A     + ++    P     +    +  ++  +   
Sbjct: 176 IFLLSRLEGDTPTLLLLVVAFTLHAYGAEFRNRIFHVYPCLSSTIFCFLSLFSIYCISDF 235

Query: 247 SAVGAVLFALLLMSISCLCPFYLIRLQLFKENIHGPWDEA 286
           S   ++ FALL + I  +CP  LI  Q  K  IHGPWDEA
Sbjct: 236 SLELSICFALLHIFILFICPLILILKQTGKCTIHGPWDEA 275
>ref|NP_181043.1| (NM_129050) putative phosphatidylinositol-glycan synthase
           [Arabidopsis thaliana]
 pir||T00479 hypothetical protein F19I3.21 - Arabidopsis thaliana
 gb|AAC12837.1| (AC004238) putative phosphatidylinositol-glycan synthase
           [Arabidopsis thaliana]
          Length = 303

 Score =  253 bits (640), Expect = 4e-66
 Identities = 98/296 (33%), Positives = 163/296 (54%), Gaps = 22/296 (7%)

Query: 13  KWQKVLY--ERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQQLCSVCVFVV 70
           KW+KV Y   +  + DNY D  FLEE+  N +  +     V+ +S  + Q LC V + V+
Sbjct: 14  KWRKVAYGGMQIGYDDNYTDESFLEEMVMNANVVRRDLLKVMKDSVSISQYLCIVALVVL 73

Query: 71  IWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADLKSALVFITFT 130
           +W +  E  L  + LL   L+    G+++  L    E +  S       L +   F T  
Sbjct: 74  VWVHTLESSLDENSLLLLDLSLLASGFLILLLT---EEKMLSLSLLLRYLVNISFFTTGL 130

Query: 131 YGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGA---------NAAIVSSTLSLNM 181
           Y  +P+ +TLT S+S+D+I+A++V +LL HL   DY               ++S +S+N 
Sbjct: 131 YILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTPNLTSCISVNA 190

Query: 182 AIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAVG 241
           +I ASV +ASRLP  LH F ++ F++Q+F   P+    +  C  +   G+ LLF+F+ +G
Sbjct: 191 SIVASVFVASRLPSRLHVFAVMLFSLQVFLFAPL----VTYCIKKFNFGLHLLFSFALMG 246

Query: 242 -GLLSISAV---GAVLFALLLMSISCLCPFYLIRLQLFKENIHGPWDEAEIKEDLS 293
             L SI A+     ++F  L++ ++ +CP++LIR+Q +K  I+GPWDEA++  D++
Sbjct: 247 LTLYSIYALHRLFFLVFLSLVLLVNVVCPYWLIRMQEYKFEINGPWDEAKLCFDIT 302
>ref|NP_015249.1| (NC_001148) Required for synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi2p
           [Saccharomyces cerevisiae]
 sp|P46961|GPI2_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI2
 pir||S61111 GPI2 protein - yeast (Saccharomyces cerevisiae)
 dbj|BAA06128.1| (D29645) Gcr4p [Saccharomyces cerevisiae]
 gb|AAB68262.1| (U41849) Gpi2p [Saccharomyces cerevisiae]
          Length = 280

 Score =  231 bits (585), Expect = 9e-60
 Identities = 51/274 (18%), Positives = 105/274 (37%), Gaps = 35/274 (12%)

Query: 11  EVKWQKVLYERQPFPDNYVDRRF--LEELRKNIHARKYQYWAVVFESSVVIQQLCSVCVF 68
              W+++L+ +Q +PDNY D  F  L   +K    +K          + +          
Sbjct: 3   RSPWKRLLWLKQEYPDNYTDPSFIELRARQKAESNQKSDRKLSEAARAQIR--------- 53

Query: 69  VVIWWYMDEGLLAPHWLLGTGLASSLIGY------VLFDLIDGGEGRKKSGQTRWA--DL 120
            + +    + +L   ++  T       G+      +    I     R K      +  D+
Sbjct: 54  -LDFISFYQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDV 112

Query: 121 KSALVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLN 180
           KS+L+        SPVLK+L+++ ++D+I+ +S ++ L ++        +    S LS N
Sbjct: 113 KSSLIITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVIS-STKSKDKPSNLSTN 171

Query: 181 MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAV 240
           + +     L+SRL  ++  F  +   IQ+  + P              V   ++   S +
Sbjct: 172 ILVALVAVLSSRLSTTIDVFCFLLICIQLNIILPT----------YLSVTNKVVPIISNI 221

Query: 241 GGLLSISAVGAVLFALLLMSISC----LCPFYLI 270
                ++     ++ LL+   S     + P + I
Sbjct: 222 IVYSFLNVALGWIYMLLIFFASVFYITVLPKWFI 255
>gb|AAA79518.1| (U23788) Gpi2p [Saccharomyces cerevisiae]
          Length = 268

 Score =  226 bits (573), Expect = 3e-58
 Identities = 53/262 (20%), Positives = 102/262 (38%), Gaps = 27/262 (10%)

Query: 11  EVKWQKVLYERQPFPDNYVDRRF--LEELRKNIHARKYQYWAVVFESSVVIQQLCSVCVF 68
              W+++L+ +Q +PDNY D  F  L   +K    +K          + +          
Sbjct: 3   RSPWKRLLWLKQEYPDNYTDPSFIELRARQKAESNQKSDRKLSEARRAQIR--------- 53

Query: 69  VVIWWYMDEGLLAPHWLLGTGLASSLIGY------VLFDLIDGGEGRKKSGQTRWA--DL 120
            + +    + +L   ++  T       G+      +    I     R K      +  D+
Sbjct: 54  -LDFISFYQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDV 112

Query: 121 KSALVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLN 180
           KS+L+        SPVLK+L+++ ++D+I+ +S ++ L ++        +    S LS N
Sbjct: 113 KSSLIITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVIS-STKSKDKPSNLSTN 171

Query: 181 MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAV 240
           + +     L+SRL  ++  F  +   IQ+  + P          P     +   F   A+
Sbjct: 172 ILVALVAVLSSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVPIISNIIVYSFLNVAL 231

Query: 241 GGLLSISAVGAVLFALLLMSIS 262
           G +        +LF+LL   I 
Sbjct: 232 GWIY------MLLFSLLQYFIL 247
 Score = 96.1 bits (237), Expect = 7e-19
 Identities = 26/150 (17%), Positives = 51/150 (33%), Gaps = 49/150 (32%)

Query: 116 RWA------------------------------------------------DLKSALVFI 127
                                                              D+KS+L+  
Sbjct: 60  YQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLIIT 119

Query: 128 TFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLNMAIFASV 187
                 SPVLK+L+++ ++D+I+ +S ++ L ++        +    S LS N+ +    
Sbjct: 120 FAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVIS-STKSKDKPSNLSTNILVALVA 178

Query: 188 CLASRLPRSLHAFIMVTFAIQIFALWPMLQ 217
            L+SRL  ++  F  +   IQ+  + P   
Sbjct: 179 VLSSRLSTTIDVFCFLLICIQLNIILPTYL 208
>gb|AAD54698.1| (AF118772) ORF5 [Equine arteritis virus]
          Length = 255

 Score = 32.6 bits (74), Expect = 8.4
 Identities = 29/176 (16%), Positives = 62/176 (34%), Gaps = 15/176 (8%)

Query: 126 FITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFD-----YGANAAIVSSTLSLN 180
           F         V+  L        +Y  S      + +F D     +G +    + ++ ++
Sbjct: 31  FTLCMLTDRGVIANLLRYDEHTALYNCSASKTCWYCVFLDEQIITFGTDCND-TYSVPVS 89

Query: 181 MAIFASVCLASRLPRSLHAFI-----MVTFAIQIFALWPMLQKKLKACTPRSYVGV-TLL 234
             +  +    S L   +  FI        F + +F  +P+L     +  P + + +   +
Sbjct: 90  TVLEQAQWPYSVLFDDMPPFIYYGREFGIFVMDVFMFYPVLVLFFLSVLPYATLILEMCV 149

Query: 235 FAFSAVGGLLSISAVGAVLFALLLMSISCLC---PFYLIRLQLFKENIHGPWDEAE 287
                V GL S + +   +FA  L+  S +      +L     ++  +H  +  AE
Sbjct: 150 SILFVVYGLYSGAYLAMGIFATTLVVHSVVVLRQLLWLCLAWRYRCTLHASFISAE 205
CPU time:    95.09 user secs.	    2.12 sys. secs	   97.21 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.319    0.177    0.511 

Gapped
Lambda     K      H
   0.270   0.0620    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 191229907
Number of Sequences: 887402
Number of extensions: 9077624
Number of successful extensions: 48028
Number of sequences better than 10.0: 610
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 601
Number of HSP's that attempted gapping in prelim test: 47388
Number of HSP's gapped (non-prelim): 993
length of query: 297
length of database: 277,845,442
effective HSP length: 52
effective length of query: 245
effective length of database: 231,700,538
effective search space: 56766631810
effective search space used: 56766631810
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.9 bits)
S2: 73 (32.4 bits)