Sequences with E-value BETTER than threshold (0.002) 367 e-100
365 e-100
362 3e-99
336 2e-91
326 2e-88
290 2e-77
270 3e-71
235 1e-60
225 1e-57
Sequences with E-value WORSE than threshold gb|AAL66816.1| (AY058990) NADH dehydrogenase subunit 2 [Demansia... 33 9.6
Alignments
>ref|NP_002633.1| (NM_002642) phosphatidylinositol glycan, class C [Homo sapiens]
ref|XP_002139.1| (XM_002139) phosphatidylinositol glycan, class C [Homo sapiens]
sp|Q92535|PIGC_HUMAN PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)
pir||JC4969 pig-c protein - human
dbj|BAA12812.1| (D85418) phosphatidylinositol-glycan-class C (PIG-C) [Homo sapiens]
emb|CAB10071.1| (Z97195) PHOSPHATIDYLINISITOL-GLYCAN class C (PIG-C) [Homo sapiens]
gb|AAH06539.1|AAH06539 (BC006539) phosphatidylinositol glycan, class C [Homo sapiens]
Length = 297
Score = 367 bits (933), Expect = e-100
Identities = 98/298 (32%), Positives = 164/298 (54%), Gaps = 22/298 (7%)
Query: 12 GPKWRKVAYGGMQIGYDDNYTDESFLEEMVMNANVVRRDLLKVMKDSVSISQYLCIVALV 71
KW+KV Y + + DNY D FLEE+ N + + V+ +S + Q LC V +
Sbjct: 11 EVKWQKVLY--ERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQQLCSVCVF 68
Query: 72 VLVWVHTLESSLDENSLLLLDLSLLASGFLILLLT---EEKMLSLSLLLRYLVNISFFTT 128
V++W + E L + LL L+ G+++ L E + S L + F T
Sbjct: 69 VVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADLKSALVFIT 128
Query: 129 GLYILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTPNLTSCISV 188
Y +P+ +TLT S+S+D+I+A++V +LL HL DY + ++S +S+
Sbjct: 129 FTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGAN---------AAIVSSTLSL 179
Query: 189 NASIVASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTY----CIKKFNFGLHLLFSFAL 244
N +I ASV +ASRLP LH F ++ F++Q+F P++ C + G+ LLF+F+
Sbjct: 180 NMAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSA 239
Query: 245 MGLTLYSIYALHRLFFLVFLSLVLLVNVVCPYWLIRMQEYKFEINGPWDEAKLCFDIT 302
+G L SI A+ + +F L++ ++ +CP++LIR+Q +K I+GPWDEA++ D++
Sbjct: 240 VG-GLLSISAVGAV---LFALLLMSISCLCPFYLIRLQLFKENIHGPWDEAEIKEDLS 293
>dbj|BAB29152.1| (AK014096) data source:SPTR, source key:Q92535,
evidence:ISS~homolog to PHOSPHATIDYLINOSITOL-GLYCAN
BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)~putative [Mus
musculus]
gb|AAH06938.1|AAH06938 (BC006938) RIKEN cDNA 3110030E07 gene [Mus musculus]
Length = 297
Score = 365 bits (929), Expect = e-100
Identities = 96/299 (32%), Positives = 158/299 (52%), Gaps = 22/299 (7%)
Query: 11 PGPKWRKVAYGGMQIGYDDNYTDESFLEEMVMNANVVRRDLLKVMKDSVSISQYLCIVAL 70
P KW+KV Y + + DNY D+ FLEE+ N + V+ +S + Q LC V +
Sbjct: 10 PEVKWQKVLY--ERQPFPDNYVDQRFLEELRKNIYARKYQYWAVVFESSVVIQQLCSVCV 67
Query: 71 VVLVWVHTLESSLDENSLLLLDLSLLASGFLILLLT---EEKMLSLSLLLRYLVNISFFT 127
V++W + E L L L+ G+++ L + + S L + F
Sbjct: 68 FVVIWWYMDEGLLAPQWLFGTGLASSLVGYVLFDLIDGGDGRKKSGRTRWADLKSTLVFI 127
Query: 128 TGLYILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTPNLTSCIS 187
T Y +P+ +TLT S+S+D+I+A+ V +LL HL DY + ++S +S
Sbjct: 128 TFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGAN---------AAIVSSTLS 178
Query: 188 VNASIVASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTY----CIKKFNFGLHLLFSFA 243
+N +I ASV +ASRLP LH F ++ F++Q+F P++ + G+ LLF+F+
Sbjct: 179 LNMAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAFS 238
Query: 244 LMGLTLYSIYALHRLFFLVFLSLVLLVNVVCPYWLIRMQEYKFEINGPWDEAKLCFDIT 302
G L SI A+ ++F L+ ++ +CPY+LI +Q +K I+GPWDEA++ D++
Sbjct: 239 AFG-GLLSISAVGA---ILFALLLFSISCLCPYYLIHLQLFKENIHGPWDEAEIKEDLS 293
>dbj|BAA22866.1| (AB000360) PIGC [Homo sapiens]
Length = 297
Score = 362 bits (922), Expect = 3e-99
Identities = 97/298 (32%), Positives = 163/298 (54%), Gaps = 22/298 (7%)
Query: 12 GPKWRKVAYGGMQIGYDDNYTDESFLEEMVMNANVVRRDLLKVMKDSVSISQYLCIVALV 71
KW+KV Y + + DNY D FLEE+ N + + V+ +S + Q LC V +
Sbjct: 11 EVKWQKVLY--ERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQQLCSVCVF 68
Query: 72 VLVWVHTLESSLDENSLLLLDLSLLASGFLILLLT---EEKMLSLSLLLRYLVNISFFTT 128
V++W + E L + LL L+ G+++ L E + S L + F T
Sbjct: 69 VVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADLKSALVFIT 128
Query: 129 GLYILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTPNLTSCISV 188
Y +P+ +TLT S+S+D+I+A++V +LL HL DY + ++S +S+
Sbjct: 129 FTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGAN---------AAIVSSTLSL 179
Query: 189 NASIVASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTY----CIKKFNFGLHLLFSFAL 244
N +I ASV +ASRLP LH F ++ F++Q+F P++ C + G+ LLF+F+
Sbjct: 180 NMAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSA 239
Query: 245 MGLTLYSIYALHRLFFLVFLSLVLLVNVVCPYWLIRMQEYKFEINGPWDEAKLCFDIT 302
+G L SI A+ + +F L++ ++ +C ++LIR+Q +K I+GPWDEA++ D++
Sbjct: 240 VG-GLLSISAVGAV---LFALLLMSISCLCSFYLIRLQLFKENIHGPWDEAEIKEDLS 293
>ref|NP_181043.1| (NM_129050) putative phosphatidylinositol-glycan synthase
[Arabidopsis thaliana]
pir||T00479 hypothetical protein F19I3.21 - Arabidopsis thaliana
gb|AAC12837.1| (AC004238) putative phosphatidylinositol-glycan synthase
[Arabidopsis thaliana]
Length = 303
Score = 336 bits (855), Expect = 2e-91
Identities = 303/303 (100%), Positives = 303/303 (100%)
Query: 1 MESNLAGDSIPGPKWRKVAYGGMQIGYDDNYTDESFLEEMVMNANVVRRDLLKVMKDSVS 60
MESNLAGDSIPGPKWRKVAYGGMQIGYDDNYTDESFLEEMVMNANVVRRDLLKVMKDSVS
Sbjct: 1 MESNLAGDSIPGPKWRKVAYGGMQIGYDDNYTDESFLEEMVMNANVVRRDLLKVMKDSVS 60
Query: 61 ISQYLCIVALVVLVWVHTLESSLDENSLLLLDLSLLASGFLILLLTEEKMLSLSLLLRYL 120
ISQYLCIVALVVLVWVHTLESSLDENSLLLLDLSLLASGFLILLLTEEKMLSLSLLLRYL
Sbjct: 61 ISQYLCIVALVVLVWVHTLESSLDENSLLLLDLSLLASGFLILLLTEEKMLSLSLLLRYL 120
Query: 121 VNISFFTTGLYILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTP 180
VNISFFTTGLYILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTP
Sbjct: 121 VNISFFTTGLYILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTP 180
Query: 181 NLTSCISVNASIVASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTYCIKKFNFGLHLLF 240
NLTSCISVNASIVASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTYCIKKFNFGLHLLF
Sbjct: 181 NLTSCISVNASIVASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTYCIKKFNFGLHLLF 240
Query: 241 SFALMGLTLYSIYALHRLFFLVFLSLVLLVNVVCPYWLIRMQEYKFEINGPWDEAKLCFD 300
SFALMGLTLYSIYALHRLFFLVFLSLVLLVNVVCPYWLIRMQEYKFEINGPWDEAKLCFD
Sbjct: 241 SFALMGLTLYSIYALHRLFFLVFLSLVLLVNVVCPYWLIRMQEYKFEINGPWDEAKLCFD 300
Query: 301 ITD 303
ITD
Sbjct: 301 ITD 303
>pir||T41379 probable phosphatidylinositol- glycan-class c protein - fission
yeast (Schizosaccharomyces pombe)
emb|CAA19108.1| (AL023592) phosphatidylinositol-glycan-class c [Schizosaccharomyces
pombe]
Length = 324
Score = 326 bits (829), Expect = 2e-88
Identities = 88/285 (30%), Positives = 146/285 (50%), Gaps = 11/285 (3%)
Query: 15 WRKVAYGGMQIGYDDNYTDESFLEEMVMNANVVRRDLLKVMKDSVSISQYLCIVALVVLV 74
W+KV + + Y DN+ DESFL + N N+ D ++ DS+ +SQ+L V + V
Sbjct: 38 WKKVLW--RKQDYPDNFIDESFLNGLQRNVNIQVTDFWSLVADSLPVSQHLSSVVIFASV 95
Query: 75 WVHTLESSLDENSLLLLDLSLLASGFLILLLTEEKMLSLSLLLRYL---VNISFFTTGLY 131
+V + L + + + F++ K + Y+ + L
Sbjct: 96 FVSIYRNQLSCALVGFVSNVSAVAAFILWDFVLRKPCNNRTFPNYMGIVKSCILIVLTLA 155
Query: 132 ILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTPNLTSCISVNAS 191
L+PI +LT+S S DS+WA+ V L L ++F H+Y+ TIR L + +S NA+
Sbjct: 156 GLSPILMSLTKSTSPDSVWAIAVWLFLANVFFHEYTTETIRP-----HVRLHNSLSTNAA 210
Query: 192 IVASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTYCIKKFNFGLHLLFSFALMGLTLYS 251
+ ASV +ASRL ++VF +LF++ F P+ I F+F +L + L+ +
Sbjct: 211 LSASVVLASRLEKSINVFFFILFAVHWFALFPIFRKYIHVFSFYADMLMTLVLIISAYIA 270
Query: 252 IYALHRLFF-LVFLSLVLLVNVVCPYWLIRMQEYKFEINGPWDEA 295
+ A+ + VFLSL+ ++ +CP W I++Q +K EI+GPWD A
Sbjct: 271 LNAVASVVIAFVFLSLIFFISFICPIWFIKLQRFKNEIHGPWDIA 315
>gb|AAF47758.1| (AE003477) CG12077 gene product [Drosophila melanogaster]
Length = 256
Score = 290 bits (737), Expect = 2e-77
Identities = 61/286 (21%), Positives = 115/286 (39%), Gaps = 50/286 (17%)
Query: 15 WRKVAYGGMQIGYDDNYTDESFLEEMVMNANVVRRDLLKVMKDSVSISQYLCIVALVVLV 74
W K Y Y DNYTD SFL+++ N + + + ++ + + +++
Sbjct: 12 WVKNLYSNR--EYPDNYTDASFLKDLRTNLHCRIYTFGEAIAGITVLNNQISCITGFLIL 69
Query: 75 WVHTLESSLDENSLLLLDLSLLASGFLILLLTEEKMLSLSLLLRYLVNISFFTTGLYILA 134
+ L S+ ++L+ + G+L + LS +LL + Y+ +
Sbjct: 70 YQLMLSDSVSPTTILVPSCGITGIGYLCY---RGRSLSWALLGEDSKTLVTVVLFGYLFS 126
Query: 135 PIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTPNLTSCISVNASIVA 194
P+ TLT++IS+D+I+ +T +LL +L Y + V A
Sbjct: 127 PMLHTLTQAISTDTIYTMTFFVLLGNLIFGHYG------------------LDVAMVSKA 168
Query: 195 SVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTYCIKKFNFGLHLLFSFALMGLTLYSIYA 254
+VF +L+ + H F + + ++Y
Sbjct: 169 AVF-------------FVLYPIMTEANW--------------HAGFMLPIFAICCMALYC 201
Query: 255 LHRLFFLVFLSLVLLVNVVCPYWLIRMQEYKFEINGPWDEAKLCFD 300
+ R ++ S + +N VCP+ + Q+YKF I+GPWDEA + +
Sbjct: 202 ISRPVLYLYASTTIFINFVCPFIFVWQQQYKFNIHGPWDEAIVEEN 247
>ref|NP_501645.1| (NM_069244) T20D3.8.p [Caenorhabditis elegans]
pir||T25032 hypothetical protein T20D3.8 - Caenorhabditis elegans
emb|CAA92490.2| (Z68220) cDNA EST yk416d12.3 comes from this gene~cDNA EST
yk416d12.5 comes from this gene~cDNA EST yk586b10.3
comes from this gene~cDNA EST yk586b10.5 comes from this
gene [Caenorhabditis elegans]
Length = 282
Score = 270 bits (684), Expect = 3e-71
Identities = 73/294 (24%), Positives = 132/294 (44%), Gaps = 38/294 (12%)
Query: 15 WRKVAYGGMQIGYDDNYT--DESFLEEMVMNANVVRRDLLKVMKDSVSISQYLCIVALVV 72
W+K+ Y + + DNY+ D FL+E+ N +VV D + ++ +L ++ +
Sbjct: 7 WQKILY--RKQPFPDNYSGGDAQFLKELRKNVSVVHYDYKSAVFGCMNFLTHLDMITMYF 64
Query: 73 LVWVHTLESSLDENSLL-----LLDLSLLASGFLILLLTEEKMLSLSLLLRYLVNISFFT 127
+++++ L S+ N L + L L FLI K + ++ ++
Sbjct: 65 VLFLNILHSNWSINILYSVFSLTIVLYLFFCKFLIPNPANAKEHARTIFTLFIFA----- 119
Query: 128 TGLYILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTPNLTSCIS 187
Y P+ +TLT SIS+D+I++ ++ + F HDY +K P ++ S
Sbjct: 120 ---YAFTPVIRTLTTSISTDTIYSTSIITAIFSCFFHDYG---------VKAPVVSYPTS 167
Query: 188 VNASIVASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTYCIKKFNFGLHL---LFSFAL 244
V+ + +++F+ SRL +++ + L Y + N H+ L S
Sbjct: 168 VSTGLSSAIFLLSRLEGDTPTLLLLVVAFT------LHAYGAEFRNRIFHVYPCLSSTIF 221
Query: 245 MGLTLYSIYALHRL---FFLVFLSLVLLVNVVCPYWLIRMQEYKFEINGPWDEA 295
L+L+SIY + + F L + + +CP LI Q K I+GPWDEA
Sbjct: 222 CFLSLFSIYCISDFSLELSICFALLHIFILFICPLILILKQTGKCTIHGPWDEA 275
>ref|NP_015249.1| (NC_001148) Required for synthesis of
N-acetylglucoaminylphosphatidylinositol, the first
intermediate in synthesis of
glycosylphosphatidylinositol (GPI) anchors; Gpi2p
[Saccharomyces cerevisiae]
sp|P46961|GPI2_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI2
pir||S61111 GPI2 protein - yeast (Saccharomyces cerevisiae)
dbj|BAA06128.1| (D29645) Gcr4p [Saccharomyces cerevisiae]
gb|AAB68262.1| (U41849) Gpi2p [Saccharomyces cerevisiae]
Length = 280
Score = 235 bits (594), Expect = 1e-60
Identities = 52/271 (19%), Positives = 114/271 (41%), Gaps = 27/271 (9%)
Query: 15 WRKVAYGGMQIGYDDNYTDESFLE--EMVMNANVVRRDLLKVMKDSVSISQYLCIVALVV 72
W+++ + + Y DNYTD SF+E + + D + ++ L ++
Sbjct: 6 WKRLLWL--KQEYPDNYTDPSFIELRARQKAESNQKSDRK--LSEAARAQIRLDFISFYQ 61
Query: 73 LVWVHTLESSLDENSLLLLDL----SLLASGFLILLLTEEKMLSLSLLLRYLVNISFFTT 128
+ ++T + + + F+ L+++ K+ L + + T
Sbjct: 62 TI-LNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLIITF 120
Query: 129 GLYILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTPNLTSCISV 188
+ L+P+ ++L+++ +SDSIW ++ L L ++F+ + S + S +S
Sbjct: 121 AMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSKDKP----------SNLST 170
Query: 189 NASIVASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTYCIKKFNFGLHLLFSFALMGLT 248
N + ++SRL + + VF +L +Q+ + P K + + ++ + +
Sbjct: 171 NILVALVAVLSSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVPIISNIIVYSFLNVA 230
Query: 249 LYSIYALHRLFFLVFLSLVLLVNVVCPYWLI 279
L IY L+F + V + V+ P W I
Sbjct: 231 LGWIY-----MLLIFFASVFYITVL-PKWFI 255
>gb|AAA79518.1| (U23788) Gpi2p [Saccharomyces cerevisiae]
Length = 268
Score = 225 bits (568), Expect = 1e-57
Identities = 48/263 (18%), Positives = 108/263 (40%), Gaps = 27/263 (10%)
Query: 15 WRKVAYGGMQIGYDDNYTDESFLE--EMVMNANVVRRDLLKVMKDSVSISQYLCIVALVV 72
W+++ + + Y DNYTD SF+E + + D + ++ L ++
Sbjct: 6 WKRLLWL--KQEYPDNYTDPSFIELRARQKAESNQKSDRK--LSEARRAQIRLDFISFYQ 61
Query: 73 LVWVHTLESSLDENSLLLLDL----SLLASGFLILLLTEEKMLSLSLLLRYLVNISFFTT 128
+ ++T + + + F+ L+++ K+ L + + T
Sbjct: 62 TI-LNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLIITF 120
Query: 129 GLYILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTPNLTSCISV 188
+ L+P+ ++L+++ +SDSIW ++ L L ++F+ + S + S +S
Sbjct: 121 AMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSKDKP----------SNLST 170
Query: 189 NASIVASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTYCIKKFNFGLHLLFSFALMGLT 248
N + ++SRL + + VF +L +Q+ + P K + + ++ + +
Sbjct: 171 NILVALVAVLSSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVPIISNIIVYSFLNVA 230
Query: 249 LYSIYALHRLFFLVFLSLVLLVN 271
L IY L+F L +
Sbjct: 231 LGWIY------MLLFSLLQYFIL 247
>gb|AAL66816.1| (AY058990) NADH dehydrogenase subunit 2 [Demansia atra]
Length = 343
Score = 32.6 bits (74), Expect = 9.6
Identities = 23/99 (23%), Positives = 39/99 (39%), Gaps = 7/99 (7%)
Query: 122 NISFFTTGLYILAPIYQTLT-RSISS----DSIWAVT-VSLLLLHLFLHDYSGSTIRAPG 175
+I F PI TL S S+ ++W + + ++ L + SG
Sbjct: 201 SILTFIIYTMTTTPILITLNHTSTSTIKDLGTVWTTSPQLMTIITLTILSLSGLPPLTGF 260
Query: 176 ALKTPNLTSCISVNASIVASVFVASRLPSRLHVFAVMLF 214
K L + IS N I A++ S L L+V+ + +
Sbjct: 261 MPKWLILNNMISTNMVIEATMMALSSL-LSLYVYIRLTY 298
CPU time: 93.03 user secs. 4.04 sys. secs 97.07 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.318 0.171 0.487
Gapped
Lambda K H
0.270 0.0598 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 180042899
Number of Sequences: 887402
Number of extensions: 7696733
Number of successful extensions: 49090
Number of sequences better than 10.0: 747
Number of HSP's better than 10.0 without gapping: 32
Number of HSP's successfully gapped in prelim test: 724
Number of HSP's that attempted gapping in prelim test: 48267
Number of HSP's gapped (non-prelim): 1258
length of query: 303
length of database: 277,845,442
effective HSP length: 54
effective length of query: 249
effective length of database: 229,925,734
effective search space: 57251507766
effective search space used: 57251507766
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.9 bits)
S2: 73 (32.5 bits)
