Sequences with E-value BETTER than threshold (0.002) 322 4e-87
303 3e-81
300 2e-80
299 4e-80
280 2e-74
278 9e-74
253 3e-66
244 9e-64
229 4e-59
Sequences with E-value WORSE than threshold gb|AAB41691.1| (U64515) hydrophobic protein [Bacillus firmus] 35 1.2
Alignments
>ref|NP_015249.1| (NC_001148) Required for synthesis of
N-acetylglucoaminylphosphatidylinositol, the first
intermediate in synthesis of
glycosylphosphatidylinositol (GPI) anchors; Gpi2p
[Saccharomyces cerevisiae]
sp|P46961|GPI2_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI2
pir||S61111 GPI2 protein - yeast (Saccharomyces cerevisiae)
dbj|BAA06128.1| (D29645) Gcr4p [Saccharomyces cerevisiae]
gb|AAB68262.1| (U41849) Gpi2p [Saccharomyces cerevisiae]
Length = 280
Score = 322 bits (818), Expect = 4e-87
Identities = 280/280 (100%), Positives = 280/280 (100%)
Query: 1 MTRSPWKRLLWLKQEYPDNYTDPSFIELRARQKAESNQKSDRKLSEAARAQIRLDFISFY 60
MTRSPWKRLLWLKQEYPDNYTDPSFIELRARQKAESNQKSDRKLSEAARAQIRLDFISFY
Sbjct: 1 MTRSPWKRLLWLKQEYPDNYTDPSFIELRARQKAESNQKSDRKLSEAARAQIRLDFISFY 60
Query: 61 QTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLIITF 120
QTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLIITF
Sbjct: 61 QTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLIITF 120
Query: 121 AMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSKDKPSNLSTNILVALVAVL 180
AMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSKDKPSNLSTNILVALVAVL
Sbjct: 121 AMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSKDKPSNLSTNILVALVAVL 180
Query: 181 SSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVPIISNIIVYSFLNVALGWIYMLLIF 240
SSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVPIISNIIVYSFLNVALGWIYMLLIF
Sbjct: 181 SSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVPIISNIIVYSFLNVALGWIYMLLIF 240
Query: 241 FASVFYITVLPKWFIYWKINYHKRDNDLLSTWDARTPILD 280
FASVFYITVLPKWFIYWKINYHKRDNDLLSTWDARTPILD
Sbjct: 241 FASVFYITVLPKWFIYWKINYHKRDNDLLSTWDARTPILD 280
>ref|NP_002633.1| (NM_002642) phosphatidylinositol glycan, class C [Homo sapiens]
ref|XP_002139.1| (XM_002139) phosphatidylinositol glycan, class C [Homo sapiens]
sp|Q92535|PIGC_HUMAN PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)
pir||JC4969 pig-c protein - human
dbj|BAA12812.1| (D85418) phosphatidylinositol-glycan-class C (PIG-C) [Homo sapiens]
emb|CAB10071.1| (Z97195) PHOSPHATIDYLINISITOL-GLYCAN class C (PIG-C) [Homo sapiens]
gb|AAH06539.1|AAH06539 (BC006539) phosphatidylinositol glycan, class C [Homo sapiens]
Length = 297
Score = 303 bits (769), Expect = 3e-81
Identities = 55/300 (18%), Positives = 109/300 (36%), Gaps = 47/300 (15%)
Query: 6 WKRLLWLKQEYPDNYTDPSFIELRARQ-----------KAESNQKSDRKLSEAARAQIRL 54
W+++L+ +Q +PDNY D F+E + ES+ + S I
Sbjct: 14 WQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQQLCSVCVFVVIWW 73
Query: 55 DFIS-------FYQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLS 107
T L +S I + I R K +
Sbjct: 74 YMDEGLLAPHWLLGTGLASSLIG----------------YVLFDLIDGGEGRKKSGQ--T 115
Query: 108 SFMDVKSSLIITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISST-KSKDKPS 166
+ D+KS+L+ SPVLK+L+++ ++D+I+ +S ++ L ++ + S
Sbjct: 116 RWADLKSALVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSS 175
Query: 167 NLSTNILVALVAVLSSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVP----IISNII 222
LS N+ + L+SRL ++ F + IQ+ + P P ++ +
Sbjct: 176 TLSLNMAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLF 235
Query: 223 VYSFLN--VALGWIYMLLIFFASVFYITVLPKWFIYWKINYHKRDNDLLSTWDARTPILD 280
+S + +++ + +L + + P + I ++ WD D
Sbjct: 236 AFSAVGGLLSISAVGAVLFALLLMSISCLCPF----YLIRLQLFKENIHGPWDEAEIKED 291
>dbj|BAB29152.1| (AK014096) data source:SPTR, source key:Q92535,
evidence:ISS~homolog to PHOSPHATIDYLINOSITOL-GLYCAN
BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)~putative [Mus
musculus]
gb|AAH06938.1|AAH06938 (BC006938) RIKEN cDNA 3110030E07 gene [Mus musculus]
Length = 297
Score = 300 bits (761), Expect = 2e-80
Identities = 54/300 (18%), Positives = 110/300 (36%), Gaps = 47/300 (15%)
Query: 6 WKRLLWLKQEYPDNYTDPSFIELRARQ-----------KAESNQKSDRKLSEAARAQIRL 54
W+++L+ +Q +PDNY D F+E + ES+ + S I
Sbjct: 14 WQKVLYERQPFPDNYVDQRFLEELRKNIYARKYQYWAVVFESSVVIQQLCSVCVFVVIWW 73
Query: 55 DFIS-------FYQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLS 107
+ T L +S + + I R K +
Sbjct: 74 YMDEGLLAPQWLFGTGLASSLVG----------------YVLFDLIDGGDGRKKSG--RT 115
Query: 108 SFMDVKSSLIITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISST-KSKDKPS 166
+ D+KS+L+ SPVLK+L+++ ++D+I+ ++ ++ L ++ + S
Sbjct: 116 RWADLKSTLVFITFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANAAIVSS 175
Query: 167 NLSTNILVALVAVLSSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVP----IISNII 222
LS N+ + L+SRL ++ F + IQ+ + P P ++ +
Sbjct: 176 TLSLNMAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLF 235
Query: 223 VYSFLN--VALGWIYMLLIFFASVFYITVLPKWFIYWKINYHKRDNDLLSTWDARTPILD 280
+S +++ + +L + P Y+ I+ ++ WD D
Sbjct: 236 AFSAFGGLLSISAVGAILFALLLFSISCLCP----YYLIHLQLFKENIHGPWDEAEIKED 291
>dbj|BAA22866.1| (AB000360) PIGC [Homo sapiens]
Length = 297
Score = 299 bits (759), Expect = 4e-80
Identities = 54/300 (18%), Positives = 109/300 (36%), Gaps = 47/300 (15%)
Query: 6 WKRLLWLKQEYPDNYTDPSFIELRARQ-----------KAESNQKSDRKLSEAARAQIRL 54
W+++L+ +Q +PDNY D F+E + ES+ + S I
Sbjct: 14 WQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQQLCSVCVFVVIWW 73
Query: 55 DFIS-------FYQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLS 107
T L +S I + I R K +
Sbjct: 74 YMDEGLLAPHWLLGTGLASSLIG----------------YVLFDLIDGGEGRKKSGQ--T 115
Query: 108 SFMDVKSSLIITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISST-KSKDKPS 166
+ D+KS+L+ SPVLK+L+++ ++D+I+ +S ++ L ++ + S
Sbjct: 116 RWADLKSALVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSS 175
Query: 167 NLSTNILVALVAVLSSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVP----IISNII 222
LS N+ + L+SRL ++ F + IQ+ + P P ++ +
Sbjct: 176 TLSLNMAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLF 235
Query: 223 VYSFLN--VALGWIYMLLIFFASVFYITVLPKWFIYWKINYHKRDNDLLSTWDARTPILD 280
+S + +++ + +L + + ++ I ++ WD D
Sbjct: 236 AFSAVGGLLSISAVGAVLFALLLMSISCLC----SFYLIRLQLFKENIHGPWDEAEIKED 291
>pir||T41379 probable phosphatidylinositol- glycan-class c protein - fission
yeast (Schizosaccharomyces pombe)
emb|CAA19108.1| (AL023592) phosphatidylinositol-glycan-class c [Schizosaccharomyces
pombe]
Length = 324
Score = 280 bits (710), Expect = 2e-74
Identities = 84/304 (27%), Positives = 136/304 (44%), Gaps = 46/304 (15%)
Query: 3 RSPWKRLLWLKQEYPDNYTDPSFIELRARQ-----------KAESNQKSDRKLSEAARAQ 51
+ PWK++LW KQ+YPDN+ D SF+ R A+S S S A
Sbjct: 35 KKPWKKVLWRKQDYPDNFIDESFLNGLQRNVNIQVTDFWSLVADSLPVSQHLSSVVIFAS 94
Query: 52 IRLDFISFYQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMD 111
+ F+S Y+ L+ + + + I F+ + P
Sbjct: 95 V---FVSIYRNQLSCALVGFV------SNVSAVAAFILWDFVLRKPCNNRTFPNYMGI-- 143
Query: 112 VKSSLIITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSKDKP-----S 166
VKS ++I + LSP+L SL+K+T+ DS+W ++ WL L +F T +P +
Sbjct: 144 VKSCILIVLTLAGLSPILMSLTKSTSPDSVWAIAVWLFLANVFFHEYTTETIRPHVRLHN 203
Query: 167 NLSTNILVALVAVLSSRLSTTIDVFCFLLICIQLNIILPT----------YLSVTNKVVP 216
+LSTN ++ VL+SRL +I+VF F+L + + P Y + +V
Sbjct: 204 SLSTNAALSASVVLASRLEKSINVFFFILFAVHWFALFPIFRKYIHVFSFYADMLMTLVL 263
Query: 217 IISNIIVYSFL-NVALGWIYMLLIFFASVFYITVLPKWFIYWKINYHKRDNDLLSTWDAR 275
IIS I + + +V + ++++ LIFF S + P WF I + N++ WD
Sbjct: 264 IISAYIALNAVASVVIAFVFLSLIFFISF----ICPIWF----IKLQRFKNEIHGPWDIA 315
Query: 276 TPIL 279
P L
Sbjct: 316 LPKL 319
>gb|AAA79518.1| (U23788) Gpi2p [Saccharomyces cerevisiae]
Length = 268
Score = 278 bits (705), Expect = 9e-74
Identities = 238/247 (96%), Positives = 239/247 (96%)
Query: 1 MTRSPWKRLLWLKQEYPDNYTDPSFIELRARQKAESNQKSDRKLSEAARAQIRLDFISFY 60
MTRSPWKRLLWLKQEYPDNYTDPSFIELRARQKAESNQKSDRKLSEA RAQIRLDFISFY
Sbjct: 1 MTRSPWKRLLWLKQEYPDNYTDPSFIELRARQKAESNQKSDRKLSEARRAQIRLDFISFY 60
Query: 61 QTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLIITF 120
QTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLIITF
Sbjct: 61 QTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLIITF 120
Query: 121 AMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSKDKPSNLSTNILVALVAVL 180
AMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSKDKPSNLSTNILVALVAVL
Sbjct: 121 AMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSKDKPSNLSTNILVALVAVL 180
Query: 181 SSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVPIISNIIVYSFLNVALGWIYMLLIF 240
SSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVPIISNIIVYSFLNVALGWIYMLL
Sbjct: 181 SSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVPIISNIIVYSFLNVALGWIYMLLFS 240
Query: 241 FASVFYI 247
F +
Sbjct: 241 LLQYFIL 247
>ref|NP_501645.1| (NM_069244) T20D3.8.p [Caenorhabditis elegans]
pir||T25032 hypothetical protein T20D3.8 - Caenorhabditis elegans
emb|CAA92490.2| (Z68220) cDNA EST yk416d12.3 comes from this gene~cDNA EST
yk416d12.5 comes from this gene~cDNA EST yk586b10.3
comes from this gene~cDNA EST yk586b10.5 comes from this
gene [Caenorhabditis elegans]
Length = 282
Score = 253 bits (640), Expect = 3e-66
Identities = 52/292 (17%), Positives = 113/292 (37%), Gaps = 33/292 (11%)
Query: 2 TRSPWKRLLWLKQEYPDNYT--DPSFIELRARQKAESNQKSDRKLSE--AARAQIRLDFI 57
T + W+++L+ KQ +PDNY+ D F++ + S D K + LD I
Sbjct: 3 TPTGWQKILYRKQPFPDNYSGGDAQFLKELRKNV--SVVHYDYKSAVFGCMNFLTHLDMI 60
Query: 58 SFYQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLI 117
+ Y +L + ++ ++ Y + ++L F +I P ++ + ++
Sbjct: 61 TMY-FVLFLNILHSNWSINILYSVFSLTIVLYLFFCKFLI------PNPANAKEHARTIF 113
Query: 118 ITFAM-LTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSK---DKPSNLSTNIL 173
F +PV+++L+ + ++D+I++ S ++ F P+++ST
Sbjct: 114 TLFIFAYAFTPVIRTLTTSISTDTIYSTSIITAIFSCFFHDYGVKAPVVSYPTSVSTG-- 171
Query: 174 VALVAVLSSRLSTTIDVFCFLLICIQLNI--------ILPTYLSVTNKVVPIISNIIVYS 225
++ L SRL L++ L+ I Y +++ + +S +Y
Sbjct: 172 LSSAIFLLSRLEGDTPTLLLLVVAFTLHAYGAEFRNRIFHVYPCLSSTIFCFLSLFSIYC 231
Query: 226 FLNVALGWIYMLLIFFASVFYITVLPKWFIYWKINYHKRDNDLLSTWDARTP 277
+ +L + +F + + P I + WD P
Sbjct: 232 ISDFSLE--LSICFALLHIFILFICPLILILK----QTGKCTIHGPWDEAVP 277
>gb|AAF47758.1| (AE003477) CG12077 gene product [Drosophila melanogaster]
Length = 256
Score = 244 bits (619), Expect = 9e-64
Identities = 50/289 (17%), Positives = 95/289 (32%), Gaps = 61/289 (21%)
Query: 3 RSPWKRLLWLKQEYPDNYTDPSFIELRARQKAESNQKSDRKLSEAARAQIRLDFISFYQT 62
R PW + L+ +EYPDNYTD SF++ EA I+
Sbjct: 9 RQPWVKNLYSNREYPDNYTDASFLKDLRTN----LHCRIYTFGEAIAG------ITVLNN 58
Query: 63 ILNTSFIYITFTYIYYYGFDPIPPTIF-----LSFITLIISRTKVDPLLSSFMDVKSSLI 117
++ Y P TI ++ I + R + D K+ +
Sbjct: 59 QISC-ITGFLILYQLMLSDSVSPTTILVPSCGITGIGYLCYRGRSLSWALLGEDSKTLVT 117
Query: 118 ITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSKDKPSNLSTNILVALV 177
+ SP+L +L++ ++D+I+T++F++ L + +V+
Sbjct: 118 VVLFGYLFSPMLHTLTQAISTDTIYTMTFFVLLGNLIFGHYGLDV---------AMVSKA 168
Query: 178 AVLSSRLSTTIDVFCFLLICI------QLNIILPTYLSVTNKVVPIISNIIVYSFLNVAL 231
AV F+L I +LP + + I + +
Sbjct: 169 AV------------FFVLYPIMTEANWHAGFMLPIFAICCMALYCI----------SRPV 206
Query: 232 GWIYMLLIFFASVFYITVLPKWFIYWKINYHKRDNDLLSTWDARTPILD 280
++Y F + V P F++ + ++ WD +
Sbjct: 207 LYLYASTTIFINF----VCPFIFVWQ----QQYKFNIHGPWDEAIVEEN 247
>ref|NP_181043.1| (NM_129050) putative phosphatidylinositol-glycan synthase
[Arabidopsis thaliana]
pir||T00479 hypothetical protein F19I3.21 - Arabidopsis thaliana
gb|AAC12837.1| (AC004238) putative phosphatidylinositol-glycan synthase
[Arabidopsis thaliana]
Length = 303
Score = 229 bits (580), Expect = 4e-59
Identities = 55/294 (18%), Positives = 117/294 (39%), Gaps = 37/294 (12%)
Query: 6 WKRLLW--LKQEYPDNYTDPSFIELRARQKAESNQKSDRKLSEAARAQIRLDFISFYQTI 63
W+++ + ++ Y DNYTD SF+E +N L ++ D +S Q +
Sbjct: 15 WRKVAYGGMQIGYDDNYTDESFLEE---MVMNANVVRRDLL------KVMKDSVSISQYL 65
Query: 64 LNTSFIYITFTYIY------YYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLI 117
+ + + + + + F+ L+++ K+ L + +
Sbjct: 66 CIVALVVLVWVHTLESSLDENSLLLLDLSLLASGFLILLLTEEKMLSLSLLLRYLVNISF 125
Query: 118 ITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSKDKP----------SN 167
T + L+P+ ++L+++ +SDSIW ++ L L ++F+ + S + S
Sbjct: 126 FTTGLYILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTPNLTSC 185
Query: 168 LSTNILVALVAVLSSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVPIISNIIVYSFL 227
+S N + ++SRL + + VF +L +Q+ + P K + + ++ +
Sbjct: 186 ISVNASIVASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTYCIKKFNFGLHLLFSFALM 245
Query: 228 NVALGWIYML------LIFFASVFYITVLPKWFIYWKINYHKRDNDLLSTWDAR 275
+ L IY L + + V P YW I + ++ WD
Sbjct: 246 GLTLYSIYALHRLFFLVFLSLVLLVNVVCP----YWLIRMQEYKFEINGPWDEA 295
>gb|AAB41691.1| (U64515) hydrophobic protein [Bacillus firmus]
Length = 567
Score = 35.3 bits (81), Expect = 1.2
Identities = 24/147 (16%), Positives = 53/147 (35%), Gaps = 20/147 (13%)
Query: 109 FMDVKSSLIITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVISSTKSKDKPSNL 168
+ D+K F M+ + P+L L ++ +L ++ + S L
Sbjct: 45 WEDIKMLFFSAFLMVAIFPILHFL--------LYLNHAFLLSLRTYMEGAGIDLHSFSVL 96
Query: 169 STNILVALVAVLSSRLSTTIDVFCFLLICIQ------LNIILPTYLSVTNKVVPIISNII 222
+TN + V++ T+ + L+ + + ++ P + + + P
Sbjct: 97 NTNAFDSFFLVIAGLAEWTLATYLNLVYFFRKILIVLIFMLAPL--ACISLLYPTTRAFF 154
Query: 223 VYSFLNVA----LGWIYMLLIFFASVF 245
++ L I+ LL FF +F
Sbjct: 155 TTWARDLTGLIFLQSIHGLLFFFILMF 181
CPU time: 78.18 user secs. 2.15 sys. secs 80.33 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.320 0.173 0.500
Gapped
Lambda K H
0.270 0.0608 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 160085566
Number of Sequences: 887402
Number of extensions: 6712386
Number of successful extensions: 44003
Number of sequences better than 10.0: 607
Number of HSP's better than 10.0 without gapping: 24
Number of HSP's successfully gapped in prelim test: 592
Number of HSP's that attempted gapping in prelim test: 43338
Number of HSP's gapped (non-prelim): 988
length of query: 280
length of database: 277,845,442
effective HSP length: 53
effective length of query: 227
effective length of database: 230,813,136
effective search space: 52394581872
effective search space used: 52394581872
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.0 bits)
S2: 73 (32.5 bits)
