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GPI Anchor Biosynthesis Report: BAB29152.1 (PIG-C family, Mus musculus)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (297 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 2

Distribution of 11 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)
Sequences producing significant alignments:
ref|NP_002633.1| (NM_002642) phosphatidylinositol glycan, class ... 431 e-119
dbj|BAB29152.1| (AK014096) data source:SPTR, source key:Q92535, ... 428 e-119
dbj|BAA22866.1| (AB000360) PIGC [Homo sapiens] 426 e-118
pir||T41379 probable phosphatidylinositol- glycan-class c protei... 345 6e-94
gb|AAF47758.1| (AE003477) CG12077 gene product [Drosophila melan... 299 6e-80
ref|NP_501645.1| (NM_069244) T20D3.8.p [Caenorhabditis elegans] ... 297 1e-79
ref|NP_181043.1| (NM_129050) putative phosphatidylinositol-glyca... 261 9e-69
ref|NP_015249.1| (NC_001148) Required for synthesis of N-acetylg... 229 5e-59
gb|AAA79518.1| (U23788) Gpi2p [Saccharomyces cerevisiae] 224 2e-57
Sequences with E-value WORSE than threshold

ref|NP_478265.1| (NC_003240) cation efflux system protein [Nosto... 36 1.2
Alignments
>ref|NP_002633.1| (NM_002642) phosphatidylinositol glycan, class C [Homo sapiens]
 ref|XP_002139.1| (XM_002139) phosphatidylinositol glycan, class C [Homo sapiens]
 sp|Q92535|PIGC_HUMAN PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)
 pir||JC4969 pig-c protein - human
 dbj|BAA12812.1| (D85418) phosphatidylinositol-glycan-class C (PIG-C) [Homo sapiens]
 emb|CAB10071.1| (Z97195) PHOSPHATIDYLINISITOL-GLYCAN class C (PIG-C) [Homo sapiens]
 gb|AAH06539.1|AAH06539 (BC006539) phosphatidylinositol glycan, class C [Homo sapiens]
          Length = 297

 Score =  431 bits (1097), Expect = e-119
 Identities = 278/297 (93%), Positives = 287/297 (96%)

Query: 1   MCAQRVTDTPEVKWQKVLYERQPFPDNYVDQRFLEELRKNIYARKYQYWAVVFESSVVIQ 60
           M AQ VT+T EVKWQKVLYERQPFPDNYVD+RFLEELRKNI+ARKYQYWAVVFESSVVIQ
Sbjct: 1   MYAQPVTNTKEVKWQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQ 60

Query: 61  QLCSVCVFVVIWWYMDEGLLAPQWLFGTGLASSLVGYVLFDLIDGGDGRKKSGRTRWADL 120
           QLCSVCVFVVIWWYMDEGLLAP WL GTGLASSL+GYVLFDLIDGG+GRKKSG+TRWADL
Sbjct: 61  QLCSVCVFVVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADL 120

Query: 121 KSTLVFITFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANAAIVSSTLSLN 180
           KS LVFITFTYGFSPVLKTLTESVSTDTIYAM+VFMLLGHLIFFDYGANAAIVSSTLSLN
Sbjct: 121 KSALVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLN 180

Query: 181 MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAFSAF 240
           MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKA TPRSYVGVTLLFAFSA 
Sbjct: 181 MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAV 240

Query: 241 GGLLSISAVGAILFALLLFSISCLCPYYLIHLQLFKENIHGPWDEAEIKEDLSRFLS 297
           GGLLSISAVGA+LFALLL SISCLCP+YLI LQLFKENIHGPWDEAEIKEDLSRFLS
Sbjct: 241 GGLLSISAVGAVLFALLLMSISCLCPFYLIRLQLFKENIHGPWDEAEIKEDLSRFLS 297
>dbj|BAB29152.1| (AK014096) data source:SPTR, source key:Q92535,
           evidence:ISS~homolog to PHOSPHATIDYLINOSITOL-GLYCAN
           BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)~putative [Mus
           musculus]
 gb|AAH06938.1|AAH06938 (BC006938) RIKEN cDNA 3110030E07 gene [Mus musculus]
          Length = 297

 Score =  428 bits (1091), Expect = e-119
 Identities = 297/297 (100%), Positives = 297/297 (100%)

Query: 1   MCAQRVTDTPEVKWQKVLYERQPFPDNYVDQRFLEELRKNIYARKYQYWAVVFESSVVIQ 60
           MCAQRVTDTPEVKWQKVLYERQPFPDNYVDQRFLEELRKNIYARKYQYWAVVFESSVVIQ
Sbjct: 1   MCAQRVTDTPEVKWQKVLYERQPFPDNYVDQRFLEELRKNIYARKYQYWAVVFESSVVIQ 60

Query: 61  QLCSVCVFVVIWWYMDEGLLAPQWLFGTGLASSLVGYVLFDLIDGGDGRKKSGRTRWADL 120
           QLCSVCVFVVIWWYMDEGLLAPQWLFGTGLASSLVGYVLFDLIDGGDGRKKSGRTRWADL
Sbjct: 61  QLCSVCVFVVIWWYMDEGLLAPQWLFGTGLASSLVGYVLFDLIDGGDGRKKSGRTRWADL 120

Query: 121 KSTLVFITFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANAAIVSSTLSLN 180
           KSTLVFITFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANAAIVSSTLSLN
Sbjct: 121 KSTLVFITFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANAAIVSSTLSLN 180

Query: 181 MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAFSAF 240
           MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAFSAF
Sbjct: 181 MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAFSAF 240

Query: 241 GGLLSISAVGAILFALLLFSISCLCPYYLIHLQLFKENIHGPWDEAEIKEDLSRFLS 297
           GGLLSISAVGAILFALLLFSISCLCPYYLIHLQLFKENIHGPWDEAEIKEDLSRFLS
Sbjct: 241 GGLLSISAVGAILFALLLFSISCLCPYYLIHLQLFKENIHGPWDEAEIKEDLSRFLS 297
>dbj|BAA22866.1| (AB000360) PIGC [Homo sapiens]
          Length = 297

 Score =  426 bits (1086), Expect = e-118
 Identities = 277/297 (93%), Positives = 286/297 (96%)

Query: 1   MCAQRVTDTPEVKWQKVLYERQPFPDNYVDQRFLEELRKNIYARKYQYWAVVFESSVVIQ 60
           M AQ VT+T EVKWQKVLYERQPFPDNYVD+RFLEELRKNI+ARKYQYWAVVFESSVVIQ
Sbjct: 1   MYAQPVTNTKEVKWQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQ 60

Query: 61  QLCSVCVFVVIWWYMDEGLLAPQWLFGTGLASSLVGYVLFDLIDGGDGRKKSGRTRWADL 120
           QLCSVCVFVVIWWYMDEGLLAP WL GTGLASSL+GYVLFDLIDGG+GRKKSG+TRWADL
Sbjct: 61  QLCSVCVFVVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADL 120

Query: 121 KSTLVFITFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANAAIVSSTLSLN 180
           KS LVFITFTYGFSPVLKTLTESVSTDTIYAM+VFMLLGHLIFFDYGANAAIVSSTLSLN
Sbjct: 121 KSALVFITFTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLN 180

Query: 181 MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAFSAF 240
           MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKA TPRSYVGVTLLFAFSA 
Sbjct: 181 MAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAV 240

Query: 241 GGLLSISAVGAILFALLLFSISCLCPYYLIHLQLFKENIHGPWDEAEIKEDLSRFLS 297
           GGLLSISAVGA+LFALLL SISCLC +YLI LQLFKENIHGPWDEAEIKEDLSRFLS
Sbjct: 241 GGLLSISAVGAVLFALLLMSISCLCSFYLIRLQLFKENIHGPWDEAEIKEDLSRFLS 297
>pir||T41379 probable phosphatidylinositol- glycan-class c protein - fission
           yeast (Schizosaccharomyces pombe)
 emb|CAA19108.1| (AL023592) phosphatidylinositol-glycan-class c [Schizosaccharomyces
           pombe]
          Length = 324

 Score =  345 bits (877), Expect = 6e-94
 Identities = 94/288 (32%), Positives = 167/288 (57%), Gaps = 7/288 (2%)

Query: 6   VTDTPEVK--WQKVLYERQPFPDNYVDQRFLEELRKNIYARKYQYWAVVFESSVVIQQLC 63
           V +  E+K  W+KVL+ +Q +PDN++D+ FL  L++N+  +   +W++V +S  V Q L 
Sbjct: 28  VKEVVELKKPWKKVLWRKQDYPDNFIDESFLNGLQRNVNIQVTDFWSLVADSLPVSQHLS 87

Query: 64  SVCVFVVIWWYMDEGLLAPQWLFGTGLASSLVGYVLFDLIDGGDGRKKSGRTRWADLKST 123
           SV +F  ++  +    L+   +      S++  ++L+D +       ++       +KS 
Sbjct: 88  SVVIFASVFVSIYRNQLSCALVGFVSNVSAVAAFILWDFVLRKPCNNRTFPNYMGIVKSC 147

Query: 124 LVFITFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANA----AIVSSTLSL 179
           ++ +    G SP+L +LT+S S D+++A+AV++ L ++ F +Y          + ++LS 
Sbjct: 148 ILIVLTLAGLSPILMSLTKSTSPDSVWAIAVWLFLANVFFHEYTTETIRPHVRLHNSLST 207

Query: 180 NMAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAFSA 239
           N A+ ASV LASRL +S++ F  + FA+  FAL+P+ +K +  ++  + + +TL+   SA
Sbjct: 208 NAALSASVVLASRLEKSINVFFFILFAVHWFALFPIFRKYIHVFSFYADMLMTLVLIISA 267

Query: 240 FGGLLSI-SAVGAILFALLLFSISCLCPYYLIHLQLFKENIHGPWDEA 286
           +  L ++ S V A +F  L+F IS +CP + I LQ FK  IHGPWD A
Sbjct: 268 YIALNAVASVVIAFVFLSLIFFISFICPIWFIKLQRFKNEIHGPWDIA 315
>gb|AAF47758.1| (AE003477) CG12077 gene product [Drosophila melanogaster]
          Length = 256

 Score =  299 bits (758), Expect = 6e-80
 Identities = 83/278 (29%), Positives = 133/278 (46%), Gaps = 42/278 (15%)

Query: 14  WQKVLYERQPFPDNYVDQRFLEELRKNIYARKYQYWAVVFESSVVIQQLCSVCVFVVIWW 73
           W K LY  + +PDNY D  FL++LR N++ R Y +   +   +V+  Q+  +  F++++ 
Sbjct: 12  WVKNLYSNREYPDNYTDASFLKDLRTNLHCRIYTFGEAIAGITVLNNQISCITGFLILYQ 71

Query: 74  YMDEGLLAPQWLFGTGLASSLVGYVLFDLIDGGDGRKKSGRTRWADLKSTLVFITFTYGF 133
            M    ++P  +       + +GY+ +       GR  S      D K+ +  + F Y F
Sbjct: 72  LMLSDSVSPTTILVPSCGITGIGYLCY------RGRSLSWALLGEDSKTLVTVVLFGYLF 125

Query: 134 SPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANAAIVSSTLSLNMAIFASVCLASRL 193
           SP+L TLT+++STDTIY M  F+LLG+LIF  YG + A+VS          A+V      
Sbjct: 126 SPMLHTLTQAISTDTIYTMTFFVLLGNLIFGHYGLDVAMVSK---------AAVF----- 171

Query: 194 PRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAFSAFGGLLSISAVGAIL 253
                           F L+P++ +            +  +FA      L  IS     L
Sbjct: 172 ----------------FVLYPIMTEANWHAGF-----MLPIFAICCM-ALYCISRPVLYL 209

Query: 254 FALLLFSISCLCPYYLIHLQLFKENIHGPWDEAEIKED 291
           +A     I+ +CP+  +  Q +K NIHGPWDEA ++E+
Sbjct: 210 YASTTIFINFVCPFIFVWQQQYKFNIHGPWDEAIVEEN 247
>ref|NP_501645.1| (NM_069244) T20D3.8.p [Caenorhabditis elegans]
 pir||T25032 hypothetical protein T20D3.8 - Caenorhabditis elegans
 emb|CAA92490.2| (Z68220) cDNA EST yk416d12.3 comes from this gene~cDNA EST
           yk416d12.5 comes from this gene~cDNA EST yk586b10.3
           comes from this gene~cDNA EST yk586b10.5 comes from this
           gene [Caenorhabditis elegans]
          Length = 282

 Score =  297 bits (754), Expect = 1e-79
 Identities = 87/280 (31%), Positives = 135/280 (48%), Gaps = 18/280 (6%)

Query: 14  WQKVLYERQPFPDNYV--DQRFLEELRKNIYARKYQYWAVVFESSVVIQQLCSVCVFVVI 71
           WQK+LY +QPFPDNY   D +FL+ELRKN+    Y Y + VF     +  L  + ++ V+
Sbjct: 7   WQKILYRKQPFPDNYSGGDAQFLKELRKNVSVVHYDYKSAVFGCMNFLTHLDMITMYFVL 66

Query: 72  WWYMDEGLLAPQW---LFGTGLASSLVGYVLF--DLIDGGDGRKKSGRTRWADLKSTLVF 126
           +      +L   W   +  +  + ++V Y+ F   LI      K+  RT           
Sbjct: 67  FL----NILHSNWSINILYSVFSLTIVLYLFFCKFLIPNPANAKEHART-------IFTL 115

Query: 127 ITFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANAAIVSSTLSLNMAIFAS 186
             F Y F+PV++TLT S+STDTIY+ ++   +    F DYG  A +VS   S++  + ++
Sbjct: 116 FIFAYAFTPVIRTLTTSISTDTIYSTSIITAIFSCFFHDYGVKAPVVSYPTSVSTGLSSA 175

Query: 187 VCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAFSAFGGLLSI 246
           + L SRL       +++  A  + A     + ++    P     +    +  +   +   
Sbjct: 176 IFLLSRLEGDTPTLLLLVVAFTLHAYGAEFRNRIFHVYPCLSSTIFCFLSLFSIYCISDF 235

Query: 247 SAVGAILFALLLFSISCLCPYYLIHLQLFKENIHGPWDEA 286
           S   +I FALL   I  +CP  LI  Q  K  IHGPWDEA
Sbjct: 236 SLELSICFALLHIFILFICPLILILKQTGKCTIHGPWDEA 275
>ref|NP_181043.1| (NM_129050) putative phosphatidylinositol-glycan synthase
           [Arabidopsis thaliana]
 pir||T00479 hypothetical protein F19I3.21 - Arabidopsis thaliana
 gb|AAC12837.1| (AC004238) putative phosphatidylinositol-glycan synthase
           [Arabidopsis thaliana]
          Length = 303

 Score =  261 bits (662), Expect = 9e-69
 Identities = 97/300 (32%), Positives = 159/300 (52%), Gaps = 22/300 (7%)

Query: 9   TPEVKWQKVLY--ERQPFPDNYVDQRFLEELRKNIYARKYQYWAVVFESSVVIQQLCSVC 66
            P  KW+KV Y   +  + DNY D+ FLEE+  N    +     V+ +S  + Q LC V 
Sbjct: 10  IPGPKWRKVAYGGMQIGYDDNYTDESFLEEMVMNANVVRRDLLKVMKDSVSISQYLCIVA 69

Query: 67  VFVVIWWYMDEGLLAPQWLFGTGLASSLVGYVLFDLIDGGDGRKKSGRTRWADLKSTLVF 126
           + V++W +  E  L    L    L+    G+++  L    + +  S       L +   F
Sbjct: 70  LVVLVWVHTLESSLDENSLLLLDLSLLASGFLILLLT---EEKMLSLSLLLRYLVNISFF 126

Query: 127 ITFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGA---------NAAIVSSTL 177
            T  Y  +P+ +TLT S+S+D+I+A+ V +LL HL   DY               ++S +
Sbjct: 127 TTGLYILAPIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTPNLTSCI 186

Query: 178 SLNMAIFASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAF 237
           S+N +I ASV +ASRLP  LH F ++ F++Q+F   P++   +K +      G+ LLF+F
Sbjct: 187 SVNASIVASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTYCIKKFNF----GLHLLFSF 242

Query: 238 SAFG-GLLSISAV---GAILFALLLFSISCLCPYYLIHLQLFKENIHGPWDEAEIKEDLS 293
           +  G  L SI A+     ++F  L+  ++ +CPY+LI +Q +K  I+GPWDEA++  D++
Sbjct: 243 ALMGLTLYSIYALHRLFFLVFLSLVLLVNVVCPYWLIRMQEYKFEINGPWDEAKLCFDIT 302
>ref|NP_015249.1| (NC_001148) Required for synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi2p
           [Saccharomyces cerevisiae]
 sp|P46961|GPI2_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI2
 pir||S61111 GPI2 protein - yeast (Saccharomyces cerevisiae)
 dbj|BAA06128.1| (D29645) Gcr4p [Saccharomyces cerevisiae]
 gb|AAB68262.1| (U41849) Gpi2p [Saccharomyces cerevisiae]
          Length = 280

 Score =  229 bits (579), Expect = 5e-59
 Identities = 51/271 (18%), Positives = 106/271 (38%), Gaps = 35/271 (12%)

Query: 14  WQKVLYERQPFPDNYVDQRF--LEELRKNIYARKYQYWAVVFESSVVIQQLCSVCVFVVI 71
           W+++L+ +Q +PDNY D  F  L   +K    +K          + +           + 
Sbjct: 6   WKRLLWLKQEYPDNYTDPSFIELRARQKAESNQKSDRKLSEAARAQIR----------LD 55

Query: 72  WWYMDEGLLAPQWLFGTGLASSLVGY------VLFDLIDGGDGRKKSGRTRWA--DLKST 123
           +    + +L   +++ T       G+      +    I     R K      +  D+KS+
Sbjct: 56  FISFYQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSS 115

Query: 124 LVFITFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANAAIVSSTLSLNMAI 183
           L+        SPVLK+L+++ ++D+I+ ++ ++ L ++        +    S LS N+ +
Sbjct: 116 LIITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVIS-STKSKDKPSNLSTNILV 174

Query: 184 FASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAFSAFGGL 243
                L+SRL  ++  F  +   IQ+  + P              V   ++   S     
Sbjct: 175 ALVAVLSSRLSTTIDVFCFLLICIQLNIILPT----------YLSVTNKVVPIISNIIVY 224

Query: 244 LSISAVGAILFALLLFSISC----LCPYYLI 270
             ++     ++ LL+F  S     + P + I
Sbjct: 225 SFLNVALGWIYMLLIFFASVFYITVLPKWFI 255
>gb|AAA79518.1| (U23788) Gpi2p [Saccharomyces cerevisiae]
          Length = 268

 Score =  224 bits (566), Expect = 2e-57
 Identities = 52/259 (20%), Positives = 103/259 (39%), Gaps = 27/259 (10%)

Query: 14  WQKVLYERQPFPDNYVDQRF--LEELRKNIYARKYQYWAVVFESSVVIQQLCSVCVFVVI 71
           W+++L+ +Q +PDNY D  F  L   +K    +K          + +           + 
Sbjct: 6   WKRLLWLKQEYPDNYTDPSFIELRARQKAESNQKSDRKLSEARRAQIR----------LD 55

Query: 72  WWYMDEGLLAPQWLFGTGLASSLVGY------VLFDLIDGGDGRKKSGRTRWA--DLKST 123
           +    + +L   +++ T       G+      +    I     R K      +  D+KS+
Sbjct: 56  FISFYQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSS 115

Query: 124 LVFITFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANAAIVSSTLSLNMAI 183
           L+        SPVLK+L+++ ++D+I+ ++ ++ L ++        +    S LS N+ +
Sbjct: 116 LIITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVIS-STKSKDKPSNLSTNILV 174

Query: 184 FASVCLASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAFSAFGGL 243
                L+SRL  ++  F  +   IQ+  + P          P     +   F   A G +
Sbjct: 175 ALVAVLSSRLSTTIDVFCFLLICIQLNIILPTYLSVTNKVVPIISNIIVYSFLNVALGWI 234

Query: 244 LSISAVGAILFALLLFSIS 262
                   +LF+LL + I 
Sbjct: 235 Y------MLLFSLLQYFIL 247
 Score = 96.5 bits (238), Expect = 6e-19
 Identities = 25/150 (16%), Positives = 51/150 (33%), Gaps = 49/150 (32%)

Query: 116 RWA------------------------------------------------DLKSTLVFI 127
                                                              D+KS+L+  
Sbjct: 60  YQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLIIT 119

Query: 128 TFTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANAAIVSSTLSLNMAIFASV 187
                 SPVLK+L+++ ++D+I+ ++ ++ L ++        +    S LS N+ +    
Sbjct: 120 FAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIFVIS-STKSKDKPSNLSTNILVALVA 178

Query: 188 CLASRLPRSLHAFIMVTFAIQIFALWPMLQ 217
            L+SRL  ++  F  +   IQ+  + P   
Sbjct: 179 VLSSRLSTTIDVFCFLLICIQLNIILPTYL 208
>ref|NP_478265.1| (NC_003240) cation efflux system protein [Nostoc sp. PCC 7120]
 dbj|BAB77261.1| (AP003602) cation efflux system protein [Nostoc sp. PCC 7120]
          Length = 1058

 Score = 35.7 bits (82), Expect = 1.2
 Identities = 24/165 (14%), Positives = 51/165 (30%), Gaps = 21/165 (12%)

Query: 72  WWYMDEGLLAPQWLFGTGLASSLVGYVLFDLIDGGDGRKKSGRTRWADLKSTLVFITFTY 131
           +  + +  +  +          +V +     + G +GR       WA L S L       
Sbjct: 434 FQIVYDTSVEVRTSVILSTVIIVVVFAPIFTLSGIEGR-IFAPMGWAYLVSILSSTLVAM 492

Query: 132 GFSPVLKTL---TESVSTDTIYAMAVFMLLGHLIFFDYGANAAIVSSTLSLNMAIFASVC 188
             SP L  +    + +  D  + +  +    +    ++           S+ +    +  
Sbjct: 493 TLSPALCAILLANQRLPDDETW-VTRWSQRLYRPLLNFSIKRP------SVVILTAIAAF 545

Query: 189 LASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTL 233
           +AS          MV F+       P  Q++    +   Y G +L
Sbjct: 546 VAS----------MVIFSSLGRVFLPEFQERTLVNSLLLYPGTSL 580
CPU time:    94.63 user secs.	    2.28 sys. secs	   96.91 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.320    0.177    0.511 

Gapped
Lambda     K      H
   0.270   0.0620    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 191477459
Number of Sequences: 887402
Number of extensions: 9127164
Number of successful extensions: 49766
Number of sequences better than 10.0: 658
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 642
Number of HSP's that attempted gapping in prelim test: 49085
Number of HSP's gapped (non-prelim): 1097
length of query: 297
length of database: 277,845,442
effective HSP length: 51
effective length of query: 246
effective length of database: 232,587,940
effective search space: 57216633240
effective search space used: 57216633240
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.9 bits)
S2: 73 (32.4 bits)