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GPI Anchor Biosynthesis Report: AAF47758.1 (PIG-C family, Drosophila melanogaster)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (256 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!

 Results of PSI-Blast iteration 2

Distribution of 9 Blast Hits on the Query Sequence

Sequences with E-value BETTER than threshold (0.002)



 gb|AAF47758.1|  (AE003477) CG12077 gene product [Drosophila melan...   382  e-105

 ref|NP_002633.1|  (NM_002642) phosphatidylinositol glycan, class ...   339  3e-92

 dbj|BAA22866.1|  (AB000360) PIGC [Homo sapiens]                        335  6e-91

 dbj|BAB29152.1|  (AK014096) data source:SPTR, source key:Q92535, ...   335  6e-91

 pir||T41379  probable phosphatidylinositol- glycan-class c protei...   280  1e-74

 ref|NP_501645.1|  (NM_069244) T20D3.8.p [Caenorhabditis elegans] ...   267  1e-70

 ref|NP_181043.1|  (NM_129050) putative phosphatidylinositol-glyca...   218  7e-56

 ref|NP_015249.1|  (NC_001148) Required for synthesis of N-acetylg...   143  3e-33

 gb|AAA79518.1|  (U23788) Gpi2p [Saccharomyces cerevisiae]              141  1e-32


Alignments


>gb|AAF47758.1| (AE003477) CG12077 gene product [Drosophila melanogaster]
          Length = 256

 Score =  382 bits (972), Expect = e-105
 Identities = 256/256 (100%), Positives = 256/256 (100%)

Query: 1   MVKNGQSKRQPWVKNLYSNREYPDNYTDASFLKDLRTNLHCRIYTFGEAIAGITVLNNQI 60
           MVKNGQSKRQPWVKNLYSNREYPDNYTDASFLKDLRTNLHCRIYTFGEAIAGITVLNNQI
Sbjct: 1   MVKNGQSKRQPWVKNLYSNREYPDNYTDASFLKDLRTNLHCRIYTFGEAIAGITVLNNQI 60

Query: 61  SCITGFLILYQLMLSDSVSPTTILVPSCGITGIGYLCYRGRSLSWALLGEDSKTLVTVVL 120
           SCITGFLILYQLMLSDSVSPTTILVPSCGITGIGYLCYRGRSLSWALLGEDSKTLVTVVL
Sbjct: 61  SCITGFLILYQLMLSDSVSPTTILVPSCGITGIGYLCYRGRSLSWALLGEDSKTLVTVVL 120

Query: 121 FGYLFSPMLHTLTQAISTDTIYTMTFFVLLGNLIFGHYGLDVAMVSKAAVFFVLYPIMTE 180
           FGYLFSPMLHTLTQAISTDTIYTMTFFVLLGNLIFGHYGLDVAMVSKAAVFFVLYPIMTE
Sbjct: 121 FGYLFSPMLHTLTQAISTDTIYTMTFFVLLGNLIFGHYGLDVAMVSKAAVFFVLYPIMTE 180

Query: 181 ANWHAGFMLPIFAICCMALYCISRPVLYLYASTTIFINFVCPFIFVWQQQYKFNIHGPWD 240
           ANWHAGFMLPIFAICCMALYCISRPVLYLYASTTIFINFVCPFIFVWQQQYKFNIHGPWD
Sbjct: 181 ANWHAGFMLPIFAICCMALYCISRPVLYLYASTTIFINFVCPFIFVWQQQYKFNIHGPWD 240

Query: 241 EAIVEENTEENLDGNL 256
           EAIVEENTEENLDGNL
Sbjct: 241 EAIVEENTEENLDGNL 256





>ref|NP_002633.1| (NM_002642) phosphatidylinositol glycan, class C [Homo sapiens]
 ref|XP_002139.1| (XM_002139) phosphatidylinositol glycan, class C [Homo sapiens]
 sp|Q92535|PIGC_HUMAN PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)
 pir||JC4969 pig-c protein - human
 dbj|BAA12812.1| (D85418) phosphatidylinositol-glycan-class C (PIG-C) [Homo sapiens]
 emb|CAB10071.1| (Z97195) PHOSPHATIDYLINISITOL-GLYCAN class C (PIG-C) [Homo sapiens]
 gb|AAH06539.1|AAH06539 (BC006539) phosphatidylinositol glycan, class C [Homo sapiens]
          Length = 297

 Score =  339 bits (862), Expect = 3e-92
 Identities = 88/283 (31%), Positives = 136/283 (47%), Gaps = 42/283 (14%)

Query: 7   SKRQPWVKNLYSNREYPDNYTDASFLKDLRTNLHCRIYTFGEAIAGITVLNNQISCITGF 66
           +K   W K LY  + +PDNY D  FL++LR N+H R Y +   +   +V+  Q+  +  F
Sbjct: 9   TKEVKWQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQQLCSVCVF 68

Query: 67  LILYQLMLSDSVSPTTILVPSCGITGIGYLCYR------GRSLSWALLGEDSKTLVTVVL 120
           ++++  M    ++P  +L      + IGY+ +       GR  S      D K+ +  + 
Sbjct: 69  VVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADLKSALVFIT 128

Query: 121 FGYLFSPMLHTLTQAISTDTIYTMTFFVLLGNLIFGHYGLDVAMVSKA-----AVFFVLY 175
           F Y FSP+L TLT+++STDTIY M+ F+LLG+LIF  YG + A+VS       A+F  + 
Sbjct: 129 FTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLNMAIFASVC 188

Query: 176 PIMTEANWHAGFMLPIFAICCMALY-------------------------------CISR 204
                      F++  FAI   AL+                                IS 
Sbjct: 189 LASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAVGGLLSISA 248

Query: 205 PVLYLYASTTIFINFVCPFIFVWQQQYKFNIHGPWDEAIVEEN 247
               L+A   + I+ +CPF  +  Q +K NIHGPWDEA ++E+
Sbjct: 249 VGAVLFALLLMSISCLCPFYLIRLQLFKENIHGPWDEAEIKED 291





>dbj|BAA22866.1| (AB000360) PIGC [Homo sapiens]
          Length = 297

 Score =  335 bits (851), Expect = 6e-91
 Identities = 87/283 (30%), Positives = 135/283 (46%), Gaps = 42/283 (14%)

Query: 7   SKRQPWVKNLYSNREYPDNYTDASFLKDLRTNLHCRIYTFGEAIAGITVLNNQISCITGF 66
           +K   W K LY  + +PDNY D  FL++LR N+H R Y +   +   +V+  Q+  +  F
Sbjct: 9   TKEVKWQKVLYERQPFPDNYVDRRFLEELRKNIHARKYQYWAVVFESSVVIQQLCSVCVF 68

Query: 67  LILYQLMLSDSVSPTTILVPSCGITGIGYLCYR------GRSLSWALLGEDSKTLVTVVL 120
           ++++  M    ++P  +L      + IGY+ +       GR  S      D K+ +  + 
Sbjct: 69  VVIWWYMDEGLLAPHWLLGTGLASSLIGYVLFDLIDGGEGRKKSGQTRWADLKSALVFIT 128

Query: 121 FGYLFSPMLHTLTQAISTDTIYTMTFFVLLGNLIFGHYGLDVAMVSKA-----AVFFVLY 175
           F Y FSP+L TLT+++STDTIY M+ F+LLG+LIF  YG + A+VS       A+F  + 
Sbjct: 129 FTYGFSPVLKTLTESVSTDTIYAMSVFMLLGHLIFFDYGANAAIVSSTLSLNMAIFASVC 188

Query: 176 PIMTEANWHAGFMLPIFAICCMALY-------------------------------CISR 204
                      F++  FAI   AL+                                IS 
Sbjct: 189 LASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKACTPRSYVGVTLLFAFSAVGGLLSISA 248

Query: 205 PVLYLYASTTIFINFVCPFIFVWQQQYKFNIHGPWDEAIVEEN 247
               L+A   + I+ +C F  +  Q +K NIHGPWDEA ++E+
Sbjct: 249 VGAVLFALLLMSISCLCSFYLIRLQLFKENIHGPWDEAEIKED 291





>dbj|BAB29152.1| (AK014096) data source:SPTR, source key:Q92535,
           evidence:ISS~homolog to PHOSPHATIDYLINOSITOL-GLYCAN
           BIOSYNTHESIS, CLASS C PROTEIN (PIG-C)~putative [Mus
           musculus]
 gb|AAH06938.1|AAH06938 (BC006938) RIKEN cDNA 3110030E07 gene [Mus musculus]
          Length = 297

 Score =  335 bits (851), Expect = 6e-91
 Identities = 83/283 (29%), Positives = 132/283 (46%), Gaps = 42/283 (14%)

Query: 7   SKRQPWVKNLYSNREYPDNYTDASFLKDLRTNLHCRIYTFGEAIAGITVLNNQISCITGF 66
           +    W K LY  + +PDNY D  FL++LR N++ R Y +   +   +V+  Q+  +  F
Sbjct: 9   TPEVKWQKVLYERQPFPDNYVDQRFLEELRKNIYARKYQYWAVVFESSVVIQQLCSVCVF 68

Query: 67  LILYQLMLSDSVSPTTILVPSCGITGIGYLCY------RGRSLSWALLGEDSKTLVTVVL 120
           ++++  M    ++P  +       + +GY+ +       GR  S      D K+ +  + 
Sbjct: 69  VVIWWYMDEGLLAPQWLFGTGLASSLVGYVLFDLIDGGDGRKKSGRTRWADLKSTLVFIT 128

Query: 121 FGYLFSPMLHTLTQAISTDTIYTMTFFVLLGNLIFGHYGLDVAMVSKA-----AVFFVLY 175
           F Y FSP+L TLT+++STDTIY M  F+LLG+LIF  YG + A+VS       A+F  + 
Sbjct: 129 FTYGFSPVLKTLTESVSTDTIYAMAVFMLLGHLIFFDYGANAAIVSSTLSLNMAIFASVC 188

Query: 176 PIMTEANWHAGFMLPIFAICCMALY-------------------------------CISR 204
                      F++  FAI   AL+                                IS 
Sbjct: 189 LASRLPRSLHAFIMVTFAIQIFALWPMLQKKLKAYTPRSYVGVTLLFAFSAFGGLLSISA 248

Query: 205 PVLYLYASTTIFINFVCPFIFVWQQQYKFNIHGPWDEAIVEEN 247
               L+A     I+ +CP+  +  Q +K NIHGPWDEA ++E+
Sbjct: 249 VGAILFALLLFSISCLCPYYLIHLQLFKENIHGPWDEAEIKED 291





>pir||T41379 probable phosphatidylinositol- glycan-class c protein - fission
           yeast (Schizosaccharomyces pombe)
 emb|CAA19108.1| (AL023592) phosphatidylinositol-glycan-class c [Schizosaccharomyces
           pombe]
          Length = 324

 Score =  280 bits (711), Expect = 1e-74
 Identities = 64/291 (21%), Positives = 129/291 (43%), Gaps = 47/291 (16%)

Query: 2   VKNGQSKRQPWVKNLYSNREYPDNYTDASFLKDLRTNLHCRIYTFGEAIAGITVLNNQIS 61
           VK     ++PW K L+  ++YPDN+ D SFL  L+ N++ ++  F   +A    ++  +S
Sbjct: 28  VKEVVELKKPWKKVLWRKQDYPDNFIDESFLNGLQRNVNIQVTDFWSLVADSLPVSQHLS 87

Query: 62  CITGFLILYQLMLSDSVSPTTILVPSCGITGIGYLCYRGRSLSWALLGED------SKTL 115
            +  F  ++  +  + +S   +   S       ++ +                    K+ 
Sbjct: 88  SVVIFASVFVSIYRNQLSCALVGFVSNVSAVAAFILWDFVLRKPCNNRTFPNYMGIVKSC 147

Query: 116 VTVVLFGYLFSPMLHTLTQAISTDTIYTMTFFVLLGNLIFGHYGLD-------------- 161
           + +VL     SP+L +LT++ S D+++ +  ++ L N+ F  Y  +              
Sbjct: 148 ILIVLTLAGLSPILMSLTKSTSPDSVWAIAVWLFLANVFFHEYTTETIRPHVRLHNSLST 207

Query: 162 -------VAMVSK-------------AAVFFVLYPIMTEA----NWHAGFMLPIFAICC- 196
                  V + S+             A  +F L+PI  +     +++A  ++ +  I   
Sbjct: 208 NAALSASVVLASRLEKSINVFFFILFAVHWFALFPIFRKYIHVFSFYADMLMTLVLIISA 267

Query: 197 -MALYCISRPVL-YLYASTTIFINFVCPFIFVWQQQYKFNIHGPWDEAIVE 245
            +AL  ++  V+ +++ S   FI+F+CP  F+  Q++K  IHGPWD A+ +
Sbjct: 268 YIALNAVASVVIAFVFLSLIFFISFICPIWFIKLQRFKNEIHGPWDIALPK 318





>ref|NP_501645.1| (NM_069244) T20D3.8.p [Caenorhabditis elegans]
 pir||T25032 hypothetical protein T20D3.8 - Caenorhabditis elegans
 emb|CAA92490.2| (Z68220) cDNA EST yk416d12.3 comes from this gene~cDNA EST
           yk416d12.5 comes from this gene~cDNA EST yk586b10.3
           comes from this gene~cDNA EST yk586b10.5 comes from this
           gene [Caenorhabditis elegans]
          Length = 282

 Score =  267 bits (678), Expect = 1e-70
 Identities = 86/271 (31%), Positives = 130/271 (47%), Gaps = 40/271 (14%)

Query: 12  WVKNLYSNREYPDNYT--DASFLKDLRTNLHCRIYTFGEAIAGITVLNNQISCITGFLIL 69
           W K LY  + +PDNY+  DA FLK+LR N+    Y +  A+ G       +  IT + +L
Sbjct: 7   WQKILYRKQPFPDNYSGGDAQFLKELRKNVSVVHYDYKSAVFGCMNFLTHLDMITMYFVL 66

Query: 70  YQLMLSDSVSPTTILVPSCGITGIGYLCY-RGRSLSWALLGEDSKTLVTVVLFGYLFSPM 128
           +  +L  + S   IL     +T + YL + +    + A   E ++T+ T+ +F Y F+P+
Sbjct: 67  FLNILHSNWS-INILYSVFSLTIVLYLFFCKFLIPNPANAKEHARTIFTLFIFAYAFTPV 125

Query: 129 LHTLTQAISTDTIYTMTFFVLLGNLIFGHYGLDVAMVS---------KAAVF-------- 171
           + TLT +ISTDTIY+ +    + +  F  YG+   +VS          +A+F        
Sbjct: 126 IRTLTTSISTDTIYSTSIITAIFSCFFHDYGVKAPVVSYPTSVSTGLSSAIFLLSRLEGD 185

Query: 172 ----FVLYPIMTEANWHAGFMLPIFA---------ICCMAL---YCISRPVLYL---YAS 212
                +L    T   + A F   IF           C ++L   YCIS   L L   +A 
Sbjct: 186 TPTLLLLVVAFTLHAYGAEFRNRIFHVYPCLSSTIFCFLSLFSIYCISDFSLELSICFAL 245

Query: 213 TTIFINFVCPFIFVWQQQYKFNIHGPWDEAI 243
             IFI F+CP I + +Q  K  IHGPWDEA+
Sbjct: 246 LHIFILFICPLILILKQTGKCTIHGPWDEAV 276





>ref|NP_181043.1| (NM_129050) putative phosphatidylinositol-glycan synthase
           [Arabidopsis thaliana]
 pir||T00479 hypothetical protein F19I3.21 - Arabidopsis thaliana
 gb|AAC12837.1| (AC004238) putative phosphatidylinositol-glycan synthase
           [Arabidopsis thaliana]
          Length = 303

 Score =  218 bits (552), Expect = 7e-56
 Identities = 61/284 (21%), Positives = 114/284 (39%), Gaps = 54/284 (19%)

Query: 11  PWVKNLYSNRE--YPDNYTDASFLKDLRTNLHCRIYTFGEAIAGITVLNNQISCITGFLI 68
            W K  Y   +  Y DNYTD SFL+++  N +       + +     ++  +  +   ++
Sbjct: 14  KWRKVAYGGMQIGYDDNYTDESFLEEMVMNANVVRRDLLKVMKDSVSISQYLCIVALVVL 73

Query: 69  LYQLMLSDSVSPTTILVPSCGITGIGYLCYRGRSLSWALLGEDSKTLVTVVLFG---YLF 125
           ++   L  S+   ++L+    +   G+L           L    + LV +  F    Y+ 
Sbjct: 74  VWVHTLESSLDENSLLLLDLSLLASGFLILLLTEEKMLSLSLLLRYLVNISFFTTGLYIL 133

Query: 126 SPMLHTLTQAISTDTIYTMTFFVLLGNLIFGHY--------------------GLDVAMV 165
           +P+  TLT++IS+D+I+ +T  +LL +L    Y                     ++ ++V
Sbjct: 134 APIYQTLTRSISSDSIWAVTVSLLLLHLFLHDYSGSTIRAPGALKTPNLTSCISVNASIV 193

Query: 166 SKAAVFFVLYPIMTEANWHAG-FMLPIFAICCMALYCISRP-----VLYLYASTTIFINF 219
             A+VF           +    F L +F    +  YCI +      +L+ +A   + +  
Sbjct: 194 --ASVFVASRLPSRLHVFAVMLFSLQVFLFAPLVTYCIKKFNFGLHLLFSFALMGLTLYS 251

Query: 220 V---------------------CPFIFVWQQQYKFNIHGPWDEA 242
           +                     CP+  +  Q+YKF I+GPWDEA
Sbjct: 252 IYALHRLFFLVFLSLVLLVNVVCPYWLIRMQEYKFEINGPWDEA 295





>ref|NP_015249.1| (NC_001148) Required for synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi2p
           [Saccharomyces cerevisiae]
 sp|P46961|GPI2_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI2
 pir||S61111 GPI2 protein - yeast (Saccharomyces cerevisiae)
 dbj|BAA06128.1| (D29645) Gcr4p [Saccharomyces cerevisiae]
 gb|AAB68262.1| (U41849) Gpi2p [Saccharomyces cerevisiae]
          Length = 280

 Score =  143 bits (358), Expect = 3e-33
 Identities = 36/157 (22%), Positives = 70/157 (43%), Gaps = 16/157 (10%)

Query: 9   RQPWVKNLYSNREYPDNYTDASFLKDLRTNLHCRIYTFGE-AIAGITVLNNQISCITGFL 67
           R PW + L+  +EYPDNYTD SF+ +LR           +  ++       ++     F+
Sbjct: 3   RSPWKRLLWLKQEYPDNYTDPSFI-ELRARQKAESNQKSDRKLSEAARAQIRLD----FI 57

Query: 68  ILYQLMLSDSVSPTTILV----------PSCGITGIGYLCYRGRSLSWALLGEDSKTLVT 117
             YQ +L+ S    T             P+  ++ I  +  R +         D K+ + 
Sbjct: 58  SFYQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLI 117

Query: 118 VVLFGYLFSPMLHTLTQAISTDTIYTMTFFVLLGNLI 154
           +       SP+L +L++  ++D+I+T++F++ L  + 
Sbjct: 118 ITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIF 154





>gb|AAA79518.1| (U23788) Gpi2p [Saccharomyces cerevisiae]
          Length = 268

 Score =  141 bits (353), Expect = 1e-32
 Identities = 36/157 (22%), Positives = 70/157 (43%), Gaps = 16/157 (10%)

Query: 9   RQPWVKNLYSNREYPDNYTDASFLKDLRTNLHCRIYTFGE-AIAGITVLNNQISCITGFL 67
           R PW + L+  +EYPDNYTD SF+ +LR           +  ++       ++     F+
Sbjct: 3   RSPWKRLLWLKQEYPDNYTDPSFI-ELRARQKAESNQKSDRKLSEARRAQIRLD----FI 57

Query: 68  ILYQLMLSDSVSPTTILV----------PSCGITGIGYLCYRGRSLSWALLGEDSKTLVT 117
             YQ +L+ S    T             P+  ++ I  +  R +         D K+ + 
Sbjct: 58  SFYQTILNTSFIYITFTYIYYYGFDPIPPTIFLSFITLIISRTKVDPLLSSFMDVKSSLI 117

Query: 118 VVLFGYLFSPMLHTLTQAISTDTIYTMTFFVLLGNLI 154
           +       SP+L +L++  ++D+I+T++F++ L  + 
Sbjct: 118 ITFAMLTLSPVLKSLSKTTASDSIWTLSFWLTLWYIF 154





CPU time:    75.24 user secs.	    2.13 sys. secs	   77.37 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.321    0.179    0.531 

Gapped
Lambda     K      H
   0.270   0.0627    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 173402216
Number of Sequences: 887402
Number of extensions: 8532130
Number of successful extensions: 40753
Number of sequences better than 10.0: 323
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 311
Number of HSP's that attempted gapping in prelim test: 40488
Number of HSP's gapped (non-prelim): 487
length of query: 256
length of database: 277,845,442
effective HSP length: 50
effective length of query: 206
effective length of database: 233,475,342
effective search space: 48095920452
effective search space used: 48095920452
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.0 bits)
S2: 73 (32.4 bits)