Sequences with E-value BETTER than threshold (0.002) 1201 0.0
963 0.0
924 0.0
430 e-119
336 1e-90
134 6e-30
Sequences with E-value WORSE than threshold sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucosyl... 43 0.025
gb|AAA36594.1| cerebroside sulfate activator protein 42 0.061
ref|NP_002769.1| prosaposin (variant Gaucher disease and vari... 42 0.061
pir||SAHUP saposin precursor [validated] - human >gi|220064|d... 42 0.061
gb|AAA36595.1| cerebroside sulfate activator protein 42 0.062
gb|AAA36596.1| cerebroside sulfate activator protein 42 0.064
emb|CAA40392.1| cerebroside sulfate activator [Homo sapiens] 42 0.064
pir||T00207 P109 protein - silkworm >gi|2575865|dbj|BAA23126.... 40 0.19
ref|XP_223521.1| similar to prosaposin (variant Gaucher disea... 40 0.19
gb|AAL54381.1|AF276996_1 lysosomal cofactor/neurotrophic fact... 39 0.28
ref|NP_571958.1| prosaposin [Danio rerio] >gi|11191997|gb|AAG... 39 0.31
dbj|BAA95677.1| prosaposin [Bos taurus] 38 0.94
gb|AAK21658.1|AF154046_1 naegleriapore A pore-forming peptide... 37 1.4
gb|AAL01157.1|AF196308_1 naegleriapore A precursor [Naegleria... 36 1.9
pir||S21770 saposin-C - bovine >gi|248087|gb|AAB21926.1| sapo... 36 2.1
sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Conta... 36 3.4
ref|NP_524597.1| Saposin-related; Aggregation promoting activ... 35 4.3
ref|NP_733408.1| CG12070-PB [Drosophila melanogaster] >gi|231... 35 4.5
gb|AAF05899.1|AF108656_1 prosaposin [Gallus gallus] 35 4.8
Alignments
>ref|NP_036184.1| acyloxyacyl hydrolase [Mus musculus]
gb|AAB81182.1| acyloxyacyl hydrolase [Mus musculus]
dbj|BAC39187.1| unnamed protein product [Mus musculus]
Length = 574
Score = 1201 bits (3073), Expect = 0.0
Identities = 574/574 (100%), Positives = 574/574 (100%)
Query: 1 MKFPWKVFKTTLLLLLLSHSLASVPSEDQPGDSYSHGQSCLGCVVLVSVIEQLAEVHNSS 60
MKFPWKVFKTTLLLLLLSHSLASVPSEDQPGDSYSHGQSCLGCVVLVSVIEQLAEVHNSS
Sbjct: 1 MKFPWKVFKTTLLLLLLSHSLASVPSEDQPGDSYSHGQSCLGCVVLVSVIEQLAEVHNSS 60
Query: 61 VQVAMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLLDEAMKADVVCYALEFCKRGAVQP 120
VQVAMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLLDEAMKADVVCYALEFCKRGAVQP
Sbjct: 61 VQVAMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLLDEAMKADVVCYALEFCKRGAVQP 120
Query: 121 QCHLYPLPQEAWESALEKARQVLRRSSTMKYPRSGRNICSLPFLTKICQKIELSIKKAVP 180
QCHLYPLPQEAWESALEKARQVLRRSSTMKYPRSGRNICSLPFLTKICQKIELSIKKAVP
Sbjct: 121 QCHLYPLPQEAWESALEKARQVLRRSSTMKYPRSGRNICSLPFLTKICQKIELSIKKAVP 180
Query: 181 FKDIDSDKHSVFPTLRGYHWRGRDCNDSDKTVYPGRRPDNWDIHQDSNCNGIWGIDPKDG 240
FKDIDSDKHSVFPTLRGYHWRGRDCNDSDKTVYPGRRPDNWDIHQDSNCNGIWGIDPKDG
Sbjct: 181 FKDIDSDKHSVFPTLRGYHWRGRDCNDSDKTVYPGRRPDNWDIHQDSNCNGIWGIDPKDG 240
Query: 241 IPYEKKFCEGSQPRGIILLGDSAGAHFHIPPEWLTASQMSVNSFLNLPSALTDELNWPQL 300
IPYEKKFCEGSQPRGIILLGDSAGAHFHIPPEWLTASQMSVNSFLNLPSALTDELNWPQL
Sbjct: 241 IPYEKKFCEGSQPRGIILLGDSAGAHFHIPPEWLTASQMSVNSFLNLPSALTDELNWPQL 300
Query: 301 SGVTGFLDSTSGIEEKSIYHRLRKRNHCNHRDYQSISKNGASSRNLKNFIESLSRNQASD 360
SGVTGFLDSTSGIEEKSIYHRLRKRNHCNHRDYQSISKNGASSRNLKNFIESLSRNQASD
Sbjct: 301 SGVTGFLDSTSGIEEKSIYHRLRKRNHCNHRDYQSISKNGASSRNLKNFIESLSRNQASD 360
Query: 361 HPAIVLYAMIGNDVCNSKADTVPEMTTPEQMYANVMQTLTHLNSHLPNGSHVILYGLPDG 420
HPAIVLYAMIGNDVCNSKADTVPEMTTPEQMYANVMQTLTHLNSHLPNGSHVILYGLPDG
Sbjct: 361 HPAIVLYAMIGNDVCNSKADTVPEMTTPEQMYANVMQTLTHLNSHLPNGSHVILYGLPDG 420
Query: 421 TFLWDSLHNRYHPLGQLNKDVTYAQFFSFLRCLQLNPCNGWMSSNKTLRTLTSERAEQLS 480
TFLWDSLHNRYHPLGQLNKDVTYAQFFSFLRCLQLNPCNGWMSSNKTLRTLTSERAEQLS
Sbjct: 421 TFLWDSLHNRYHPLGQLNKDVTYAQFFSFLRCLQLNPCNGWMSSNKTLRTLTSERAEQLS 480
Query: 481 NTLKKIATTETFANFDLFYVDFAFHEIIEDWQKRGGQPWQLIEPVDGFHPNEVASLLQAN 540
NTLKKIATTETFANFDLFYVDFAFHEIIEDWQKRGGQPWQLIEPVDGFHPNEVASLLQAN
Sbjct: 481 NTLKKIATTETFANFDLFYVDFAFHEIIEDWQKRGGQPWQLIEPVDGFHPNEVASLLQAN 540
Query: 541 RVWEKIQLQWPHVLGKENPFNSQIEEVFGDQGGH 574
RVWEKIQLQWPHVLGKENPFNSQIEEVFGDQGGH
Sbjct: 541 RVWEKIQLQWPHVLGKENPFNSQIEEVFGDQGGH 574
>ref|NP_001628.1| acyloxyacyl hydrolase precursor [Homo sapiens]
sp|P28039|AOAH_HUMAN Acyloxyacyl hydrolase precursor
pir||A40292 acyloxyacyl hydrolase (EC 3.1.1.-), neutrophil precursor
[validated] - human
gb|AAA35506.1| acyloxyacyl hydrolase
gb|AAH25698.1| acyloxyacyl hydrolase (neutrophil) [Homo sapiens]
Length = 575
Score = 963 bits (2464), Expect = 0.0
Identities = 437/575 (76%), Positives = 512/575 (89%), Gaps = 1/575 (0%)
Query: 1 MKFPWKVFKTTLLLLLLSHSLASVPS-EDQPGDSYSHGQSCLGCVVLVSVIEQLAEVHNS 59
M+ PWK+ L LLLS ++ P+ +DQ S S+G +C+GCV++VSVIEQLA+VHNS
Sbjct: 1 MQSPWKILTVAPLFLLLSLQSSASPANDDQSRPSLSNGHTCVGCVLVVSVIEQLAQVHNS 60
Query: 60 SVQVAMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLLDEAMKADVVCYALEFCKRGAVQ 119
+VQ +MERLCSYLPEKLFLKT CY ++ FGSDIIKLL M ADVVC+ LEFCK+ Q
Sbjct: 61 TVQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKLLSADMNADVVCHTLEFCKQNTGQ 120
Query: 120 PQCHLYPLPQEAWESALEKARQVLRRSSTMKYPRSGRNICSLPFLTKICQKIELSIKKAV 179
P CHLYPLP+E W+ L+KARQ++++S +KY RSG +ICSLP L KICQKI+L+++++V
Sbjct: 121 PLCHLYPLPKETWKFTLQKARQIVKKSPILKYSRSGSDICSLPVLAKICQKIKLAMEQSV 180
Query: 180 PFKDIDSDKHSVFPTLRGYHWRGRDCNDSDKTVYPGRRPDNWDIHQDSNCNGIWGIDPKD 239
PFKD+DSDK+SVFPTLRGYHWRGRDCNDSD++VYPGRRP+NWD+HQDSNCNGIWG+DPKD
Sbjct: 181 PFKDVDSDKYSVFPTLRGYHWRGRDCNDSDESVYPGRRPNNWDVHQDSNCNGIWGVDPKD 240
Query: 240 GIPYEKKFCEGSQPRGIILLGDSAGAHFHIPPEWLTASQMSVNSFLNLPSALTDELNWPQ 299
G+PYEKKFCEGSQPRGIILLGDSAGAHFHI PEW+TASQMS+NSF+NLP+ALT+EL+WPQ
Sbjct: 241 GVPYEKKFCEGSQPRGIILLGDSAGAHFHISPEWITASQMSLNSFINLPTALTNELDWPQ 300
Query: 300 LSGVTGFLDSTSGIEEKSIYHRLRKRNHCNHRDYQSISKNGASSRNLKNFIESLSRNQAS 359
LSG TGFLDST GI+EKSIY RL KRNHCNHRDYQ+IS+NGASSRNLK FIESLSRN+
Sbjct: 301 LSGATGFLDSTVGIKEKSIYLRLWKRNHCNHRDYQNISRNGASSRNLKKFIESLSRNKVL 360
Query: 360 DHPAIVLYAMIGNDVCNSKADTVPEMTTPEQMYANVMQTLTHLNSHLPNGSHVILYGLPD 419
D+PAIV+YAMIGNDVC+ K+D VP MTTPE++Y+NVMQTL HLNSHLPNGSHVILYGLPD
Sbjct: 361 DYPAIVIYAMIGNDVCSGKSDPVPAMTTPEKLYSNVMQTLKHLNSHLPNGSHVILYGLPD 420
Query: 420 GTFLWDSLHNRYHPLGQLNKDVTYAQFFSFLRCLQLNPCNGWMSSNKTLRTLTSERAEQL 479
GTFLWD+LHNRYHPLGQLNKD+TYAQ +SFL CLQ++PC+GWMSSNKTLRTLTSERAEQL
Sbjct: 421 GTFLWDNLHNRYHPLGQLNKDMTYAQLYSFLNCLQVSPCHGWMSSNKTLRTLTSERAEQL 480
Query: 480 SNTLKKIATTETFANFDLFYVDFAFHEIIEDWQKRGGQPWQLIEPVDGFHPNEVASLLQA 539
SNTLKKIA +E F NF+LFY+DFAFHEII++WQKRGGQPWQLIEPVDGFHPNEVA LL A
Sbjct: 481 SNTLKKIAASEKFTNFNLFYMDFAFHEIIQEWQKRGGQPWQLIEPVDGFHPNEVALLLLA 540
Query: 540 NRVWEKIQLQWPHVLGKENPFNSQIEEVFGDQGGH 574
+ W+K+QLQWP +LGKENPFN QI++VFGDQGGH
Sbjct: 541 DHFWKKVQLQWPQILGKENPFNPQIKQVFGDQGGH 575
>gb|AAB81183.1| acyloxyacyl hydrolase [Oryctolagus cuniculus]
Length = 575
Score = 924 bits (2362), Expect = 0.0
Identities = 424/575 (73%), Positives = 491/575 (84%), Gaps = 1/575 (0%)
Query: 1 MKFPWKVFKTTLLLLLLSHSLASVPSEDQPGDSYSHGQSCLGCVVLVSVIEQLAEVHNSS 60
MK PW++ + LLLL HS S ++QPG S +C+GCV++VSVIEQLA+VHNS+
Sbjct: 1 MKSPWRILVVSPLLLLPLHSSTSRAHDNQPGTIRSDHYTCVGCVLVVSVIEQLAQVHNST 60
Query: 61 VQVAMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLLDEAMKADVVCYALEFCKRGAVQP 120
VQ +MERLCSYLPE+ LKTACY +V FG+DIIKL D+ + ADVVC+ LEFCK+ QP
Sbjct: 61 VQASMERLCSYLPEEWVLKTACYMMVHVFGADIIKLFDKDVNADVVCHTLEFCKQEPGQP 120
Query: 121 QCHLYPLPQEAWESALEKARQVLRRSSTMKYPRSGRNICSLPFLTKICQKIELSIKKAVP 180
CHLYPLP+E+W+ LEKAR ++++S MKY RSG ICSLPFL KICQKI+L+IK +VP
Sbjct: 121 LCHLYPLPKESWKFTLEKARHIVKQSPIMKYTRSGAGICSLPFLAKICQKIKLAIKNSVP 180
Query: 181 FKDIDSDKHSVFPTLRGYHWRGRDCNDSDKTVYPGRRPDNWDIHQDSNCNGIWGIDPKDG 240
KD+DSDK+S+FPTLRGYHWRGRDCNDSDKTVYPGRRPDNWD H+DSNCNGIWG+DPKDG
Sbjct: 181 IKDVDSDKYSIFPTLRGYHWRGRDCNDSDKTVYPGRRPDNWDAHRDSNCNGIWGVDPKDG 240
Query: 241 IPYEKKFCEGSQPRGIILLGDSAGAHFHIPPEWLTASQMSVNSFLNLPSALTDELNWPQL 300
IPYEKKFCEGSQPRGIILLGDSAGAHFHIPPEWLT SQMSVNSFLNLP+A+T+EL+WPQL
Sbjct: 241 IPYEKKFCEGSQPRGIILLGDSAGAHFHIPPEWLTVSQMSVNSFLNLPTAVTNELDWPQL 300
Query: 301 SGVTGFLDSTSGIEEKSIYHRLRKRNHCNHRDYQSISKNGASSRNLKNFIESLSRNQASD 360
SG TGFLDS S I+E SIY RLRKRN CNHRDYQ+ISKNGASSRN+K+ IESLSRNQ D
Sbjct: 301 SGTTGFLDSASKIKENSIYLRLRKRNRCNHRDYQNISKNGASSRNVKSLIESLSRNQLLD 360
Query: 361 HPAIVLYAMIGNDVCNS-KADTVPEMTTPEQMYANVMQTLTHLNSHLPNGSHVILYGLPD 419
HPAIV+YAMIGNDVCN K D V MTTPEQ+YANV++ L LNSHLP GSHVILYGL
Sbjct: 361 HPAIVIYAMIGNDVCNGRKTDPVSAMTTPEQLYANVLKMLEALNSHLPTGSHVILYGLAH 420
Query: 420 GTFLWDSLHNRYHPLGQLNKDVTYAQFFSFLRCLQLNPCNGWMSSNKTLRTLTSERAEQL 479
G FLWD+LH+RYHPLGQLNKDVTY Q +SFL CLQ++PC GWMS+N+TLR LTSERA+QL
Sbjct: 421 GAFLWDTLHSRYHPLGQLNKDVTYTQLYSFLGCLQVSPCPGWMSANETLRALTSERAQQL 480
Query: 480 SNTLKKIATTETFANFDLFYVDFAFHEIIEDWQKRGGQPWQLIEPVDGFHPNEVASLLQA 539
S TL+KIA ++ F NF+LFY+DFAF E++E+WQK GGQPW+LIE VDGFHPNEVA LL A
Sbjct: 481 SETLRKIAASKKFTNFNLFYLDFAFQEVVEEWQKMGGQPWELIEAVDGFHPNEVALLLFA 540
Query: 540 NRVWEKIQLQWPHVLGKENPFNSQIEEVFGDQGGH 574
+++WEK+Q QWP VLGKENPFN QIEEVFGDQGGH
Sbjct: 541 DQLWEKVQRQWPDVLGKENPFNPQIEEVFGDQGGH 575
>gb|AAM43641.1| ACYLOXYACYL HYDROLASE [Dictyostelium discoideum]
Length = 564
Score = 430 bits (1094), Expect = e-119
Identities = 217/543 (39%), Positives = 322/543 (58%), Gaps = 27/543 (4%)
Query: 33 SYSHGQSCLGCVVLVSVIEQLAEVHNSSVQVAMERLCSYLPEKLFLKTACYFLVQTFGSD 92
S + G C C +++S++ Q + +HN S++ +++ LCS+ P++ + C +LV T+G
Sbjct: 47 SANGGTHCAACTIVLSLVNQYSGIHNKSIEDSLDELCSFFPDE--AGSLCTWLVNTYGEQ 104
Query: 93 IIKLLDEAMKADVVCYALEFCKRGAVQPQCHLY-PLPQEAWESALEKARQVLRRSSTMKY 151
II D+ AD VC+A+ C P C L+ P + + + Q +Y
Sbjct: 105 IISYFDQYQNADDVCHAMNVC----TSPTCRLFTSAPSKFYNP--DHVHQ--------EY 150
Query: 152 PRSGRNICSLPFLTKICQKIELSIKKAVPFKDIDSDKHSVFPTLRGYHWRGRDCNDSDKT 211
+ + P+ I + I++ + +P +D D D HS+ T RGY+WRG+DCND DK+
Sbjct: 151 NKEDPKVGGSPW-AWIQELIKVFSSEHLPIQDFDGDHHSLEATFRGYNWRGKDCNDFDKS 209
Query: 212 VYPGRRPDNWDIHQDSNCNGIWGIDPKDGIPYEKKFCEGSQPRGIILLGDSAGAHFHIPP 271
YPG D D NCNGI+G++ + YE+ FC S G+I+ GDSAGAHF IPP
Sbjct: 210 SYPGTIEGTGDASVDKNCNGIFGVNNQSQ-SYEELFCSNSGAMGVIVSGDSAGAHFSIPP 268
Query: 272 EWLTASQMSVNSFLNLPSALTDELNWPQLSGVTGFLDSTSGIEEKSIYHRLRKRNHCNHR 331
+W+TA ++ ++ + L E++WP S TG+ TS S+Y R+R+RN CNHR
Sbjct: 269 QWMTARDINSTTYKGMIDILETEVDWPMRSCYTGW--DTSAPNVDSMYLRMRERNLCNHR 326
Query: 332 DYQSISKNGASSRNLKNFIESLSRNQASDHPAIVLYAMIGNDVCNSKADTVPEMTTPEQM 391
DYQ++ NGASS + + I++++RNQ +D P ++ +IGNDVC+ D MTT E+
Sbjct: 327 DYQNLGVNGASSSSTRTIIQTINRNQNTDKPVLLFLELIGNDVCSGHHD-YDSMTTVEEF 385
Query: 392 YANVMQTLTHLNSHLPNGSHVILYGLPDGTFLWDSLHNRYHPLGQLNKDVTYAQFFSFLR 451
N++ L +L + LP GSHV+ GL DG LWDSL NR HPLG TY + + FL
Sbjct: 386 GENILTILNYLETTLPKGSHVVFVGLADGRVLWDSLWNRTHPLG-----CTYEEVYDFLN 440
Query: 452 CLQLNPCNGWMSSNKTLRTLTSERAEQLSNTLKKIATTETFANFDLFYVDFAFHEIIEDW 511
C+Q++PC GWM+ N+T+R TS RA QLS ++ TF NFD+ Y DF F I E W
Sbjct: 441 CIQVSPCWGWMNPNETVRDYTSNRAAQLSLVYNQLIKNYTFNNFDMIYYDFPFATINEMW 500
Query: 512 QKRGGQPWQLIEPVDGFHPNEVASLLQANRVWEKIQLQWPHVLGKENPFNSQIEEVFGDQ 571
+GGQ + LIEP+DGFHPN++A+ L A + +I P LG+ N +N+QI+ +FG+Q
Sbjct: 501 VAQGGQTYDLIEPIDGFHPNQIANYLMAGVFYNQIAQDKPEWLGEINQYNTQIQSIFGNQ 560
Query: 572 GGH 574
GG+
Sbjct: 561 GGY 563
>emb|CAC50079.1| GPI inositol deacylase [Trypanosoma brucei brucei]
Length = 558
Score = 336 bits (852), Expect = 1e-90
Identities = 199/581 (34%), Positives = 315/581 (53%), Gaps = 47/581 (8%)
Query: 7 VFKTTLLLLLLSHSLASVPSEDQPGDSYSHGQS--CLGCVVLVSVIEQLAEVHNSSVQVA 64
+F L L ++ S+ + +E + S + C+ C +V+ + QL ++H + A
Sbjct: 12 LFTALLQLTVVCPSIHTAKAEARESKSLGSSDTWGCVLCTAVVATVSQLGQLHQIPAKDA 71
Query: 65 MERLCSYL--PEKLFLKTACYFLVQTFGSDIIKLLDEAMKADVVCYALEFCKRGAVQPQC 122
+ CS+ E + KTA L++ ++L+DE D VC +++C +C
Sbjct: 72 LGLFCSFFRSAEAVLCKTASLILLE----GALQLIDEGRTPDKVCQIMKYCD----TEEC 123
Query: 123 HLYPLPQEAWESALEKARQVLRRSSTMKYPRSGRNIC-SLPFLTKICQKI-ELSIKKAVP 180
L+P P + L K R R R + C + P L C+ + E S
Sbjct: 124 ILFP-PDKNDTLPLHKLRSAYRLDG-----RKLVDACRTFPTL---CRPLFEDSA----- 169
Query: 181 FKDIDSDKHSVFPTLRGYHWRGRDCNDSDKTVYPGRRPDNWDIHQDSNCNGIWGIDPKDG 240
D D D+ S +P RG WRGRDC+D D VYPGR ++ D +D NCNGI+G++
Sbjct: 170 -HDKDGDRFSTYPAKRGTDWRGRDCDDDDARVYPGR--NSTDSQRDENCNGIYGVEESSK 226
Query: 241 IPYEKKFCEGSQPRGIILLGDSAGAHFHIPPEWLTASQMSVNSFLNLPSALTDELNWPQL 300
YE+ +C S P G+I +GDSA A F IP +++ +S + N+ L +E++WP L
Sbjct: 227 KTYEELWCSNSSPMGVIAVGDSATADFGIPLGYVSILDLSSEAVANIIPVLDNEIDWPML 286
Query: 301 SGVTGFLDSTSGIEE-----KSIYHRLRKRNHCNHRDYQSISKNGASSRNLKNFIESLSR 355
SG+TGF +ST + S+Y +L + N CNHRDYQ++ NGA S ++ + ++S++R
Sbjct: 287 SGLTGFANSTRYEPDVDGPMTSVYSKLVELNRCNHRDYQNLGHNGARSGSILSILDSVAR 346
Query: 356 NQA-SDHPAIVLYAMIGNDVCNSKADTVPEMTTPEQMYANVMQTLTHLNSHLPNGSHVIL 414
N++ S PA V ++M+GNDVC D TP Y ++ + + +S LP GSHV++
Sbjct: 347 NRSESVKPAYVFFSMVGNDVCPRPQDA----PTPLDYYHSLSEAVKKADSFLPPGSHVLI 402
Query: 415 YGLPDGTFLWDSLHNRYHPLGQLNKDVTYAQFFSFLRCLQLNPCNGWMSSNKTLRTLTSE 474
L D S+ NR HP+G LN DVTY + +L CLQ++PC GW++SN+++R T E
Sbjct: 403 VSLVDARAAIGSIVNRIHPIGTLNSDVTYGNLYDYLNCLQVSPCWGWLNSNESVRNETWE 462
Query: 475 RAEQLSNTLKKIATTET-FANFDLFYVDFAFHEIIEDWQKRGGQPWQLIEPVDGFHPNEV 533
RA +++ + ++ T N + + E++ ++ G W+L+ PVDGFHP+++
Sbjct: 463 RASAMNSMISRVVDESTNLENVKVHALGDVIGEVVSSFE---GPLWKLVNPVDGFHPSQI 519
Query: 534 ASLLQANRVWEKIQLQWPHVLGKENPFNSQIEEVFGDQGGH 574
A+ L RV+ K+Q +L ENPFN I+ FGDQGG+
Sbjct: 520 ATALMGARVFAKMQDL--GILPTENPFNGDIQRRFGDQGGY 558
>ref|XP_225370.1| similar to acyloxyacyl hydrolase [Mus musculus] [Rattus norvegicus]
Length = 212
Score = 134 bits (335), Expect = 6e-30
Identities = 55/59 (93%), Positives = 57/59 (96%)
Query: 175 IKKAVPFKDIDSDKHSVFPTLRGYHWRGRDCNDSDKTVYPGRRPDNWDIHQDSNCNGIW 233
+KKAVPFKD DSDK+SVFPTLRGYHWRGRDCND DKTVYPGRRPDNWDIHQDSNCNGIW
Sbjct: 1 MKKAVPFKDGDSDKYSVFPTLRGYHWRGRDCNDKDKTVYPGRRPDNWDIHQDSNCNGIW 59
>sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucosylceramidase activator)
(Sphingolipid activator protein 2) (SAP-2)
pir||A32026 glucosylceramide beta-glucosidase activator protein SAP-2 - guinea
pig
Length = 81
Score = 42.7 bits (99), Expect = 0.025
Identities = 22/80 (27%), Positives = 42/80 (52%), Gaps = 4/80 (5%)
Query: 39 SCLGCVVLVSVIEQLAEVHNSSVQV--AMERLCSYLPEKLFLKTACYFLVQTFGSDIIKL 96
+C C +V + +L + + + ++ A++ +C+ LPE + C +V T+G I+ L
Sbjct: 4 TCKACEYVVKKVMELIDNNRTEEKIIHALDSVCALLPES--VSEVCQEVVDTYGDSIVAL 61
Query: 97 LDEAMKADVVCYALEFCKRG 116
L + M ++VC L C G
Sbjct: 62 LLQEMSPELVCSELGLCMSG 81
>gb|AAA36594.1| cerebroside sulfate activator protein
Length = 527
Score = 41.5 bits (96), Expect = 0.061
Identities = 23/76 (30%), Positives = 41/76 (53%), Gaps = 4/76 (5%)
Query: 40 CLGCVVLVSVIEQLAEVHNSSVQV--AMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLL 97
C C LV + +L + + + ++ A +++CS LP+ L C +V T+GS I+ +L
Sbjct: 318 CEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKS--LSEECQEVVDTYGSSILSIL 375
Query: 98 DEAMKADVVCYALEFC 113
E + ++VC L C
Sbjct: 376 LEEVSPELVCSMLHLC 391
>ref|NP_002769.1| prosaposin (variant Gaucher disease and variant metachromatic
leukodystrophy); Prosaposin (sphingolipid activator
protein-1) [Homo sapiens]
sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
gb|AAA52560.1| co-beta glucosidase precursor
gb|AAB59494.1| sphingolipid activator precursor
gb|AAA60303.1| prosaposin
gb|AAH04275.1|AAH04275 prosaposin (variant Gaucher disease and variant metachromatic
leukodystrophy) [Homo sapiens]
gb|AAH07612.1|AAH07612 prosaposin (variant Gaucher disease and variant metachromatic
leukodystrophy) [Homo sapiens]
gb|AAH01503.1|AAH01503 prosaposin (variant Gaucher disease and variant metachromatic
leukodystrophy) [Homo sapiens]
Length = 524
Score = 41.5 bits (96), Expect = 0.061
Identities = 23/76 (30%), Positives = 41/76 (53%), Gaps = 4/76 (5%)
Query: 40 CLGCVVLVSVIEQLAEVHNSSVQV--AMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLL 97
C C LV + +L + + + ++ A +++CS LP+ L C +V T+GS I+ +L
Sbjct: 315 CEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKS--LSEECQEVVDTYGSSILSIL 372
Query: 98 DEAMKADVVCYALEFC 113
E + ++VC L C
Sbjct: 373 LEEVSPELVCSMLHLC 388
>pir||SAHUP saposin precursor [validated] - human
dbj|BAA00321.1| sphingolipid activator proteins [Homo sapiens]
prf||1504251A sphingolipid activator
Length = 527
Score = 41.5 bits (96), Expect = 0.061
Identities = 23/76 (30%), Positives = 41/76 (53%), Gaps = 4/76 (5%)
Query: 40 CLGCVVLVSVIEQLAEVHNSSVQV--AMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLL 97
C C LV + +L + + + ++ A +++CS LP+ L C +V T+GS I+ +L
Sbjct: 318 CEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKS--LSEECQEVVDTYGSSILSIL 375
Query: 98 DEAMKADVVCYALEFC 113
E + ++VC L C
Sbjct: 376 LEEVSPELVCSMLHLC 391
>gb|AAA36595.1| cerebroside sulfate activator protein
Length = 524
Score = 41.5 bits (96), Expect = 0.062
Identities = 23/76 (30%), Positives = 41/76 (53%), Gaps = 4/76 (5%)
Query: 40 CLGCVVLVSVIEQLAEVHNSSVQV--AMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLL 97
C C LV + +L + + + ++ A +++CS LP+ L C +V T+GS I+ +L
Sbjct: 315 CEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKS--LSEECQEVVDTYGSSILSIL 372
Query: 98 DEAMKADVVCYALEFC 113
E + ++VC L C
Sbjct: 373 LEEVSPELVCSMLHLC 388
>gb|AAA36596.1| cerebroside sulfate activator protein
Length = 526
Score = 41.5 bits (96), Expect = 0.064
Identities = 23/76 (30%), Positives = 41/76 (53%), Gaps = 4/76 (5%)
Query: 40 CLGCVVLVSVIEQLAEVHNSSVQV--AMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLL 97
C C LV + +L + + + ++ A +++CS LP+ L C +V T+GS I+ +L
Sbjct: 317 CEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKS--LSEECQEVVDTYGSSILSIL 374
Query: 98 DEAMKADVVCYALEFC 113
E + ++VC L C
Sbjct: 375 LEEVSPELVCSMLHLC 390
>emb|CAA40392.1| cerebroside sulfate activator [Homo sapiens]
Length = 210
Score = 41.5 bits (96), Expect = 0.064
Identities = 23/76 (30%), Positives = 41/76 (53%), Gaps = 4/76 (5%)
Query: 40 CLGCVVLVSVIEQLAEVHNSSVQV--AMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLL 97
C C LV + +L + + + ++ A +++CS LP+ L C +V T+GS I+ +L
Sbjct: 12 CEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKS--LSEECQEVVDTYGSSILSIL 69
Query: 98 DEAMKADVVCYALEFC 113
E + ++VC L C
Sbjct: 70 LEEVSPELVCSMLHLC 85
>pir||T00207 P109 protein - silkworm
dbj|BAA23126.1| BmP109 [Bombyx mori]
Length = 965
Score = 40.0 bits (92), Expect = 0.19
Identities = 23/85 (27%), Positives = 46/85 (54%), Gaps = 4/85 (4%)
Query: 32 DSYSHGQSCLGCVVLVSVIEQLAEVHNS--SVQVAMERLCSYLPEKLFLKTACYFLVQTF 89
D S +C C+ V +E + + + S +++ A++ LC+ L +K L++ C V T+
Sbjct: 633 DVNSEKSNCPLCLFAVEQLESVLKNNRSEENIRKALDGLCTRLSQK--LQSECIDFVDTY 690
Query: 90 GSDIIKLLDEAMKADVVCYALEFCK 114
S ++++L M A +C L+ C+
Sbjct: 691 SSQLVEMLVADMNAKEICVFLKLCR 715
Score = 36.1 bits (82), Expect = 2.8
Identities = 21/82 (25%), Positives = 40/82 (48%), Gaps = 4/82 (4%)
Query: 35 SHGQSCLGC-VVLVSVIEQLAEVHN-SSVQVAMERLCSYLPEKLFLKTACYFLVQTFGSD 92
S C+ C VL + +Q+ + HN ++ A+ +C ++P+ + C V+ +
Sbjct: 782 SQSNVCVLCEFVLKEIDDQIKDKHNDDEIKKAVHGICKHMPKS--VSAECDQFVEKYADL 839
Query: 93 IIKLLDEAMKADVVCYALEFCK 114
+I LL + + VC L+ CK
Sbjct: 840 VISLLAQELDPSEVCEELKLCK 861
>ref|XP_223521.1| similar to prosaposin (variant Gaucher disease and variant
metachromatic leukodystrophy); Prosaposin (sphingolipid
activator protein-1) [Homo sapiens] [Rattus norvegicus]
Length = 527
Score = 39.6 bits (91), Expect = 0.19
Identities = 22/79 (27%), Positives = 46/79 (57%), Gaps = 5/79 (6%)
Query: 37 GQSCLGCVVLVSVIEQLAEVHNSSVQV--AMERLCSYLPEKLFLKTACYFLVQTFGSDII 94
G +C C+ +V +++ E +++ + A+ER+CS +PE L + C LV T+G +++
Sbjct: 297 GLTCDVCLNVVQELDKWLETNSTEALISHALERVCSIMPESLVQQ--CITLVDTYGPNLV 354
Query: 95 KLLDEAMKADVVCYALEFC 113
+L+ + + + VC + C
Sbjct: 355 QLVSK-VTPEKVCETIXLC 372
>gb|AAL54381.1|AF276996_1 lysosomal cofactor/neurotrophic factor prosaposin [Danio rerio]
Length = 520
Score = 39.2 bits (90), Expect = 0.28
Identities = 23/76 (30%), Positives = 37/76 (48%), Gaps = 4/76 (5%)
Query: 40 CLGCVVLVSVIEQLAEVHNSSVQV--AMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLL 97
C C ++ IE + + S ++ A+E++C+ LP L C L++T+G II LL
Sbjct: 312 CAICEYVMKEIENMIQDQTSEAEIVQAVEKVCNILPST--LTAQCKDLIETYGQAIIDLL 369
Query: 98 DEAMKADVVCYALEFC 113
+ VC L C
Sbjct: 370 VQEADPKTVCSFLALC 385
Score = 35.3 bits (80), Expect = 4.4
Identities = 36/129 (27%), Positives = 63/129 (47%), Gaps = 10/129 (7%)
Query: 9 KTTLLLLLLSHSLASVPSEDQPGDSYSHGQSCLGCVVLVSVIEQLAEVH--NSSVQVAME 66
KT L L ++ VP D+ ++ G C C + V ++ + E + S ++ A+
Sbjct: 376 KTVCSFLALCSGVSHVPVMDK--QHFAAGGFCDVCKMAVRYVDGILEQNATQSEIEEAVL 433
Query: 67 RLCSYLPEKLFLKTACYFLVQTFGSDIIKLLDEAMKADVVCYALEFCKRGAVQPQCHLYP 126
++CS+LP+ +K C L++ + +++LL + + D VC L C AVQ L
Sbjct: 434 KVCSFLPDA--VKDECNQLIEQYEPLLVQLLLQTLDPDFVCMKLGACPE-AVQ---RLLG 487
Query: 127 LPQEAWESA 135
L Q +W A
Sbjct: 488 LNQCSWGPA 496
>ref|NP_571958.1| prosaposin [Danio rerio]
gb|AAG32919.1|AF108655_1 prosaposin precursor [Danio rerio]
Length = 522
Score = 39.2 bits (90), Expect = 0.31
Identities = 23/76 (30%), Positives = 37/76 (48%), Gaps = 4/76 (5%)
Query: 40 CLGCVVLVSVIEQLAEVHNSSVQV--AMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLL 97
C C ++ IE + + S ++ A+E++C+ LP L C L++T+G II LL
Sbjct: 314 CAICEYVMKEIENMIQDQTSEAEIVQAVEKVCNILPST--LTAQCKDLIETYGQAIIDLL 371
Query: 98 DEAMKADVVCYALEFC 113
+ VC L C
Sbjct: 372 VQEADPKTVCSFLALC 387
>dbj|BAA95677.1| prosaposin [Bos taurus]
Length = 525
Score = 37.6 bits (86), Expect = 0.94
Identities = 21/76 (27%), Positives = 40/76 (52%), Gaps = 4/76 (5%)
Query: 40 CLGCVVLVSVIEQLAEVHNSSVQV--AMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLL 97
C C +V + +L + + + ++ A++++CS LP L C +V T+GS I+ +L
Sbjct: 316 CEVCEFVVKEVAKLIDNNRTEEEILHALDKVCSKLPTS--LAEQCQEVVDTYGSSILSIL 373
Query: 98 DEAMKADVVCYALEFC 113
+ ++VC L C
Sbjct: 374 LDEASPELVCSMLHLC 389
>gb|AAK21658.1|AF154046_1 naegleriapore A pore-forming peptide precursor [Naegleria fowleri]
Length = 307
Score = 36.9 bits (84), Expect = 1.4
Identities = 42/153 (27%), Positives = 67/153 (43%), Gaps = 21/153 (13%)
Query: 21 LASVPSED---QPGDSYSHGQSCLGCVVLVSVIEQLAEVHNSSVQVAME--RLCSYLPEK 75
+ +VP E+ GD+ C C ++ +E E ++S ++ E +LCS +P
Sbjct: 103 VVTVPKEEVKATAGDA-----ECEICKFVIQQVEAFIESNHSQAEIQKELNKLCSSVPS- 156
Query: 76 LFLKTACYFLVQTFGSDIIKLLDEAMKADVVCYALEFCKRGAV------QPQCHLYPL-P 128
+F +T C + + IIK L+E VC L CK AV Q L L P
Sbjct: 157 IFTQT-CLSIARMVPY-IIKKLEEHNSPGQVCQGLHLCKSSAVKTMPTFQVDAELKGLIP 214
Query: 129 QEAWESALEKARQVLRRSSTMKYPRSG-RNICS 160
A A+E Q + +SS + +N+C+
Sbjct: 215 CPACLMAMELVEQEISQSSVQSFVEDKLKNVCA 247
>gb|AAL01157.1|AF196308_1 naegleriapore A precursor [Naegleria fowleri]
Length = 294
Score = 36.5 bits (83), Expect = 1.9
Identities = 42/153 (27%), Positives = 67/153 (43%), Gaps = 21/153 (13%)
Query: 21 LASVPSED---QPGDSYSHGQSCLGCVVLVSVIEQLAEVHNSSVQVAME--RLCSYLPEK 75
+ +VP E+ GD+ C C ++ +E E ++S ++ E +LCS +P
Sbjct: 90 VVTVPKEEVKATAGDA-----ECEICKFVIQQVEAFIESNHSQAEIQKELNKLCSSVPS- 143
Query: 76 LFLKTACYFLVQTFGSDIIKLLDEAMKADVVCYALEFCKRGAV------QPQCHLYPL-P 128
+F +T C + + IIK L+E VC L CK AV Q L L P
Sbjct: 144 IFTQT-CLSIARMVPY-IIKKLEEHNSPGQVCQGLHLCKSSAVKTMPTFQVDAELKGLIP 201
Query: 129 QEAWESALEKARQVLRRSSTMKYPRSG-RNICS 160
A A+E Q + +SS + +N+C+
Sbjct: 202 CPACLMAMELVEQEISQSSVQSFVEDKLKNVCA 234
>pir||S21770 saposin-C - bovine
gb|AAB21926.1| saposin-C, co-glucosidase, sphingolipid activator protein A,
SAP-2=activator protein [cattle, spleen, Peptide, 80 aa]
Length = 80
Score = 36.5 bits (83), Expect = 2.1
Identities = 20/76 (26%), Positives = 39/76 (51%), Gaps = 4/76 (5%)
Query: 40 CLGCVVLVSVIEQLAEVHNSSVQV--AMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLL 97
C C +V + +L + + + ++ A++++CS LP L C +V T+G I+ +L
Sbjct: 5 CQVCEFVVKEVAKLIDNNRTEEEILHALDKVCSKLPTS--LAEQCQEVVDTYGRSILSIL 62
Query: 98 DEAMKADVVCYALEFC 113
+ ++VC L C
Sbjct: 63 LDEASPELVCSMLHLC 78
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 525
Score = 35.7 bits (81), Expect = 3.4
Identities = 20/76 (26%), Positives = 39/76 (51%), Gaps = 4/76 (5%)
Query: 40 CLGCVVLVSVIEQLAEVHNSSVQV--AMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLL 97
C C +V + +L + + + ++ A++++CS LP L C +V T+G I+ +L
Sbjct: 316 CEVCEFVVKEVAKLIDNNRTEEEILHALDKVCSKLPTS--LAEQCQEVVDTYGRSILSIL 373
Query: 98 DEAMKADVVCYALEFC 113
+ ++VC L C
Sbjct: 374 LDEASPELVCSMLHLC 389
>ref|NP_524597.1| Saposin-related; Aggregation promoting activity; Drosophila
Aggregation promoting activity; 110kD protein
[Drosophila melanogaster]
gb|AAD38622.1|AF145647_1 BcDNA.GH08312 [Drosophila melanogaster]
gb|AAF57097.1| CG12070-PA [Drosophila melanogaster]
Length = 953
Score = 35.3 bits (80), Expect = 4.3
Identities = 23/96 (23%), Positives = 44/96 (44%), Gaps = 4/96 (4%)
Query: 20 SLASVPSEDQPGDSYSHGQSCLGCVVLVSVIEQLAEVHNS--SVQVAMERLCSYLPEKLF 77
SL +P + ++ +CL C LV +E+ H + S++ +E C + +
Sbjct: 646 SLEELPPQLAFDPGFTAAPNCLICEELVKTLEKRMGKHPTRDSIKHILEESCDRMRKP-- 703
Query: 78 LKTACYFLVQTFGSDIIKLLDEAMKADVVCYALEFC 113
+ T C+ ++ +G I LL + M ++C L C
Sbjct: 704 MNTKCHKVIDKYGDKIADLLLKEMDPKLICTELGMC 739
>ref|NP_733408.1| CG12070-PB [Drosophila melanogaster]
gb|AAN14261.1|AE003775_32 CG12070-PB [Drosophila melanogaster]
Length = 469
Score = 35.3 bits (80), Expect = 4.5
Identities = 23/96 (23%), Positives = 44/96 (44%), Gaps = 4/96 (4%)
Query: 20 SLASVPSEDQPGDSYSHGQSCLGCVVLVSVIEQLAEVHNS--SVQVAMERLCSYLPEKLF 77
SL +P + ++ +CL C LV +E+ H + S++ +E C + +
Sbjct: 162 SLEELPPQLAFDPGFTAAPNCLICEELVKTLEKRMGKHPTRDSIKHILEESCDRMRKP-- 219
Query: 78 LKTACYFLVQTFGSDIIKLLDEAMKADVVCYALEFC 113
+ T C+ ++ +G I LL + M ++C L C
Sbjct: 220 MNTKCHKVIDKYGDKIADLLLKEMDPKLICTELGMC 255
>gb|AAF05899.1|AF108656_1 prosaposin [Gallus gallus]
Length = 518
Score = 35.3 bits (80), Expect = 4.8
Identities = 20/79 (25%), Positives = 40/79 (50%), Gaps = 4/79 (5%)
Query: 37 GQSCLGCVVLVSVIEQLAEVHNSSVQVA--MERLCSYLPEKLFLKTACYFLVQTFGSDII 94
G C C ++V+ ++ E + ++ ++ +E++C +LPE + C V+ + ++
Sbjct: 400 GGFCDICKMIVAYADKELEKNATTTEIEALLEKVCHFLPES--VSDQCVQFVEQYEPVVV 457
Query: 95 KLLDEAMKADVVCYALEFC 113
+LL E M VC L C
Sbjct: 458 QLLAEMMDPTFVCTKLGVC 476
CPU time: 140.78 user secs. 2.45 sys. secs 143.23 total secs.
Database: nr
Posted date: Jan 18, 2003 1:23 PM
Number of letters in database: 421,297,171
Number of sequences in database: 1,315,992
Lambda K H
0.320 0.136 0.426
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 501775739
Number of Sequences: 1315992
Number of extensions: 21572179
Number of successful extensions: 47807
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 27
Number of HSP's that attempted gapping in prelim test: 47774
Number of HSP's gapped (non-prelim): 42
length of query: 574
length of database: 421,297,171
effective HSP length: 56
effective length of query: 518
effective length of database: 347,601,619
effective search space: 180057638642
effective search space used: 180057638642
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 77 (34.4 bits)
