Sequences with E-value BETTER than threshold (0.002) 1191 0.0
974 0.0
964 0.0
429 e-118
348 2e-94
128 3e-28
49 3e-04
Sequences with E-value WORSE than threshold sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucosyl... 43 0.014
ref|XP_223521.1| similar to prosaposin (variant Gaucher disea... 42 0.031
dbj|BAA95677.1| prosaposin [Bos taurus] 40 0.15
ref|NP_524597.1| Saposin-related; Aggregation promoting activ... 40 0.21
ref|NP_733408.1| CG12070-PB [Drosophila melanogaster] >gi|231... 40 0.22
emb|CAA40392.1| cerebroside sulfate activator [Homo sapiens] 38 0.52
pir||SAHUP saposin precursor [validated] - human >gi|220064|d... 38 0.52
gb|AAA36594.1| cerebroside sulfate activator protein 38 0.52
ref|NP_002769.1| prosaposin (variant Gaucher disease and vari... 38 0.52
gb|AAA36595.1| cerebroside sulfate activator protein 38 0.53
sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Conta... 38 0.55
gb|AAA36596.1| cerebroside sulfate activator protein 38 0.58
ref|NP_491803.1| Putative secreted or extracellular protein p... 38 0.67
gb|AAL92313.1| Proteasome subunit beta type 1 [Dictyostelium ... 37 1.1
pir||S21770 saposin-C - bovine >gi|248087|gb|AAB21926.1| sapo... 37 1.2
gb|AAL96718.1| COUNTIN [Dictyostelium discoideum] 37 1.6
gb|AAK77913.1|AF400074_2 surfactant, pulmonary-associated pro... 36 2.4
ref|NP_000533.1| surfactant, pulmonary-associated protein B; ... 36 2.4
Alignments
>ref|NP_001628.1| acyloxyacyl hydrolase precursor [Homo sapiens]
sp|P28039|AOAH_HUMAN Acyloxyacyl hydrolase precursor
pir||A40292 acyloxyacyl hydrolase (EC 3.1.1.-), neutrophil precursor
[validated] - human
gb|AAA35506.1| acyloxyacyl hydrolase
gb|AAH25698.1| acyloxyacyl hydrolase (neutrophil) [Homo sapiens]
Length = 575
Score = 1191 bits (3049), Expect = 0.0
Identities = 575/575 (100%), Positives = 575/575 (100%)
Query: 1 MQSPWKILTVAPLFLLLSLQSSASPANDDQSRPSLSNGHTCVGCVLVVSVIEQLAQVHNS 60
MQSPWKILTVAPLFLLLSLQSSASPANDDQSRPSLSNGHTCVGCVLVVSVIEQLAQVHNS
Sbjct: 1 MQSPWKILTVAPLFLLLSLQSSASPANDDQSRPSLSNGHTCVGCVLVVSVIEQLAQVHNS 60
Query: 61 TVQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKLLSADMNADVVCHTLEFCKQNTGQ 120
TVQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKLLSADMNADVVCHTLEFCKQNTGQ
Sbjct: 61 TVQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKLLSADMNADVVCHTLEFCKQNTGQ 120
Query: 121 PLCHLYPLPKETWKFTLQKARQIVKKSPILKYSRSGSDICSLPVLAKICQKIKLAMEQSV 180
PLCHLYPLPKETWKFTLQKARQIVKKSPILKYSRSGSDICSLPVLAKICQKIKLAMEQSV
Sbjct: 121 PLCHLYPLPKETWKFTLQKARQIVKKSPILKYSRSGSDICSLPVLAKICQKIKLAMEQSV 180
Query: 181 PFKDVDSDKYSVFPTLRGYHWRGRDCNDSDESVYPGRRPNNWDVHQDSNCNGIWGVDPKD 240
PFKDVDSDKYSVFPTLRGYHWRGRDCNDSDESVYPGRRPNNWDVHQDSNCNGIWGVDPKD
Sbjct: 181 PFKDVDSDKYSVFPTLRGYHWRGRDCNDSDESVYPGRRPNNWDVHQDSNCNGIWGVDPKD 240
Query: 241 GVPYEKKFCEGSQPRGIILLGDSAGAHFHISPEWITASQMSLNSFINLPTALTNELDWPQ 300
GVPYEKKFCEGSQPRGIILLGDSAGAHFHISPEWITASQMSLNSFINLPTALTNELDWPQ
Sbjct: 241 GVPYEKKFCEGSQPRGIILLGDSAGAHFHISPEWITASQMSLNSFINLPTALTNELDWPQ 300
Query: 301 LSGATGFLDSTVGIKEKSIYLRLWKRNHCNHRDYQNISRNGASSRNLKKFIESLSRNKVL 360
LSGATGFLDSTVGIKEKSIYLRLWKRNHCNHRDYQNISRNGASSRNLKKFIESLSRNKVL
Sbjct: 301 LSGATGFLDSTVGIKEKSIYLRLWKRNHCNHRDYQNISRNGASSRNLKKFIESLSRNKVL 360
Query: 361 DYPAIVIYAMIGNDVCSGKSDPVPAMTTPEKLYSNVMQTLKHLNSHLPNGSHVILYGLPD 420
DYPAIVIYAMIGNDVCSGKSDPVPAMTTPEKLYSNVMQTLKHLNSHLPNGSHVILYGLPD
Sbjct: 361 DYPAIVIYAMIGNDVCSGKSDPVPAMTTPEKLYSNVMQTLKHLNSHLPNGSHVILYGLPD 420
Query: 421 GTFLWDNLHNRYHPLGQLNKDMTYAQLYSFLNCLQVSPCHGWMSSNKTLRTLTSERAEQL 480
GTFLWDNLHNRYHPLGQLNKDMTYAQLYSFLNCLQVSPCHGWMSSNKTLRTLTSERAEQL
Sbjct: 421 GTFLWDNLHNRYHPLGQLNKDMTYAQLYSFLNCLQVSPCHGWMSSNKTLRTLTSERAEQL 480
Query: 481 SNTLKKIAASEKFTNFNLFYMDFAFHEIIQEWQKRGGQPWQLIEPVDGFHPNEVALLLLA 540
SNTLKKIAASEKFTNFNLFYMDFAFHEIIQEWQKRGGQPWQLIEPVDGFHPNEVALLLLA
Sbjct: 481 SNTLKKIAASEKFTNFNLFYMDFAFHEIIQEWQKRGGQPWQLIEPVDGFHPNEVALLLLA 540
Query: 541 DHFWKKVQLQWPQILGKENPFNPQIKQVFGDQGGH 575
DHFWKKVQLQWPQILGKENPFNPQIKQVFGDQGGH
Sbjct: 541 DHFWKKVQLQWPQILGKENPFNPQIKQVFGDQGGH 575
>gb|AAB81183.1| acyloxyacyl hydrolase [Oryctolagus cuniculus]
Length = 575
Score = 974 bits (2492), Expect = 0.0
Identities = 453/576 (78%), Positives = 513/576 (88%), Gaps = 2/576 (0%)
Query: 1 MQSPWKILTVAPLFLLLSLQSSASPANDDQSRPSLSNGHTCVGCVLVVSVIEQLAQVHNS 60
M+SPW+IL V+PL LL S+ S A+D+Q S+ +TCVGCVLVVSVIEQLAQVHNS
Sbjct: 1 MKSPWRILVVSPLLLLPLHSST-SRAHDNQPGTIRSDHYTCVGCVLVVSVIEQLAQVHNS 59
Query: 61 TVQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKLLSADMNADVVCHTLEFCKQNTGQ 120
TVQASMERLCSYLPE+ LKT CY+++ FG+DIIKL D+NADVVCHTLEFCKQ GQ
Sbjct: 60 TVQASMERLCSYLPEEWVLKTACYMMVHVFGADIIKLFDKDVNADVVCHTLEFCKQEPGQ 119
Query: 121 PLCHLYPLPKETWKFTLQKARQIVKKSPILKYSRSGSDICSLPVLAKICQKIKLAMEQSV 180
PLCHLYPLPKE+WKFTL+KAR IVK+SPI+KY+RSG+ ICSLP LAKICQKIKLA++ SV
Sbjct: 120 PLCHLYPLPKESWKFTLEKARHIVKQSPIMKYTRSGAGICSLPFLAKICQKIKLAIKNSV 179
Query: 181 PFKDVDSDKYSVFPTLRGYHWRGRDCNDSDESVYPGRRPNNWDVHQDSNCNGIWGVDPKD 240
P KDVDSDKYS+FPTLRGYHWRGRDCNDSD++VYPGRRP+NWD H+DSNCNGIWGVDPKD
Sbjct: 180 PIKDVDSDKYSIFPTLRGYHWRGRDCNDSDKTVYPGRRPDNWDAHRDSNCNGIWGVDPKD 239
Query: 241 GVPYEKKFCEGSQPRGIILLGDSAGAHFHISPEWITASQMSLNSFINLPTALTNELDWPQ 300
G+PYEKKFCEGSQPRGIILLGDSAGAHFHI PEW+T SQMS+NSF+NLPTA+TNELDWPQ
Sbjct: 240 GIPYEKKFCEGSQPRGIILLGDSAGAHFHIPPEWLTVSQMSVNSFLNLPTAVTNELDWPQ 299
Query: 301 LSGATGFLDSTVGIKEKSIYLRLWKRNHCNHRDYQNISRNGASSRNLKKFIESLSRNKVL 360
LSG TGFLDS IKE SIYLRL KRN CNHRDYQNIS+NGASSRN+K IESLSRN++L
Sbjct: 300 LSGTTGFLDSASKIKENSIYLRLRKRNRCNHRDYQNISKNGASSRNVKSLIESLSRNQLL 359
Query: 361 DYPAIVIYAMIGNDVCSG-KSDPVPAMTTPEKLYSNVMQTLKHLNSHLPNGSHVILYGLP 419
D+PAIVIYAMIGNDVC+G K+DPV AMTTPE+LY+NV++ L+ LNSHLP GSHVILYGL
Sbjct: 360 DHPAIVIYAMIGNDVCNGRKTDPVSAMTTPEQLYANVLKMLEALNSHLPTGSHVILYGLA 419
Query: 420 DGTFLWDNLHNRYHPLGQLNKDMTYAQLYSFLNCLQVSPCHGWMSSNKTLRTLTSERAEQ 479
G FLWD LH+RYHPLGQLNKD+TY QLYSFL CLQVSPC GWMS+N+TLR LTSERA+Q
Sbjct: 420 HGAFLWDTLHSRYHPLGQLNKDVTYTQLYSFLGCLQVSPCPGWMSANETLRALTSERAQQ 479
Query: 480 LSNTLKKIAASEKFTNFNLFYMDFAFHEIIQEWQKRGGQPWQLIEPVDGFHPNEVALLLL 539
LS TL+KIAAS+KFTNFNLFY+DFAF E+++EWQK GGQPW+LIE VDGFHPNEVALLL
Sbjct: 480 LSETLRKIAASKKFTNFNLFYLDFAFQEVVEEWQKMGGQPWELIEAVDGFHPNEVALLLF 539
Query: 540 ADHFWKKVQLQWPQILGKENPFNPQIKQVFGDQGGH 575
AD W+KVQ QWP +LGKENPFNPQI++VFGDQGGH
Sbjct: 540 ADQLWEKVQRQWPDVLGKENPFNPQIEEVFGDQGGH 575
>ref|NP_036184.1| acyloxyacyl hydrolase [Mus musculus]
gb|AAB81182.1| acyloxyacyl hydrolase [Mus musculus]
dbj|BAC39187.1| unnamed protein product [Mus musculus]
Length = 574
Score = 964 bits (2465), Expect = 0.0
Identities = 437/575 (76%), Positives = 512/575 (89%), Gaps = 1/575 (0%)
Query: 1 MQSPWKILTVAPLFLLLSLQSSASPANDDQSRPSLSNGHTCVGCVLVVSVIEQLAQVHNS 60
M+ PWK+ L LLLS ++ P+ +DQ S S+G +C+GCV++VSVIEQLA+VHNS
Sbjct: 1 MKFPWKVFKTTLLLLLLSHSLASVPS-EDQPGDSYSHGQSCLGCVVLVSVIEQLAEVHNS 59
Query: 61 TVQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKLLSADMNADVVCHTLEFCKQNTGQ 120
+VQ +MERLCSYLPEKLFLKT CY ++ FGSDIIKLL M ADVVC+ LEFCK+ Q
Sbjct: 60 SVQVAMERLCSYLPEKLFLKTACYFLVQTFGSDIIKLLDEAMKADVVCYALEFCKRGAVQ 119
Query: 121 PLCHLYPLPKETWKFTLQKARQIVKKSPILKYSRSGSDICSLPVLAKICQKIKLAMEQSV 180
P CHLYPLP+E W+ L+KARQ++++S +KY RSG +ICSLP L KICQKI+L+++++V
Sbjct: 120 PQCHLYPLPQEAWESALEKARQVLRRSSTMKYPRSGRNICSLPFLTKICQKIELSIKKAV 179
Query: 181 PFKDVDSDKYSVFPTLRGYHWRGRDCNDSDESVYPGRRPNNWDVHQDSNCNGIWGVDPKD 240
PFKD+DSDK+SVFPTLRGYHWRGRDCNDSD++VYPGRRP+NWD+HQDSNCNGIWG+DPKD
Sbjct: 180 PFKDIDSDKHSVFPTLRGYHWRGRDCNDSDKTVYPGRRPDNWDIHQDSNCNGIWGIDPKD 239
Query: 241 GVPYEKKFCEGSQPRGIILLGDSAGAHFHISPEWITASQMSLNSFINLPTALTNELDWPQ 300
G+PYEKKFCEGSQPRGIILLGDSAGAHFHI PEW+TASQMS+NSF+NLP+ALT+EL+WPQ
Sbjct: 240 GIPYEKKFCEGSQPRGIILLGDSAGAHFHIPPEWLTASQMSVNSFLNLPSALTDELNWPQ 299
Query: 301 LSGATGFLDSTVGIKEKSIYLRLWKRNHCNHRDYQNISRNGASSRNLKKFIESLSRNKVL 360
LSG TGFLDST GI+EKSIY RL KRNHCNHRDYQ+IS+NGASSRNLK FIESLSRN+
Sbjct: 300 LSGVTGFLDSTSGIEEKSIYHRLRKRNHCNHRDYQSISKNGASSRNLKNFIESLSRNQAS 359
Query: 361 DYPAIVIYAMIGNDVCSGKSDPVPAMTTPEKLYSNVMQTLKHLNSHLPNGSHVILYGLPD 420
D+PAIV+YAMIGNDVC+ K+D VP MTTPE++Y+NVMQTL HLNSHLPNGSHVILYGLPD
Sbjct: 360 DHPAIVLYAMIGNDVCNSKADTVPEMTTPEQMYANVMQTLTHLNSHLPNGSHVILYGLPD 419
Query: 421 GTFLWDNLHNRYHPLGQLNKDMTYAQLYSFLNCLQVSPCHGWMSSNKTLRTLTSERAEQL 480
GTFLWD+LHNRYHPLGQLNKD+TYAQ +SFL CLQ++PC+GWMSSNKTLRTLTSERAEQL
Sbjct: 420 GTFLWDSLHNRYHPLGQLNKDVTYAQFFSFLRCLQLNPCNGWMSSNKTLRTLTSERAEQL 479
Query: 481 SNTLKKIAASEKFTNFNLFYMDFAFHEIIQEWQKRGGQPWQLIEPVDGFHPNEVALLLLA 540
SNTLKKIA +E F NF+LFY+DFAFHEII++WQKRGGQPWQLIEPVDGFHPNEVA LL A
Sbjct: 480 SNTLKKIATTETFANFDLFYVDFAFHEIIEDWQKRGGQPWQLIEPVDGFHPNEVASLLQA 539
Query: 541 DHFWKKVQLQWPQILGKENPFNPQIKQVFGDQGGH 575
+ W+K+QLQWP +LGKENPFN QI++VFGDQGGH
Sbjct: 540 NRVWEKIQLQWPHVLGKENPFNSQIEEVFGDQGGH 574
>gb|AAM43641.1| ACYLOXYACYL HYDROLASE [Dictyostelium discoideum]
Length = 564
Score = 429 bits (1091), Expect = e-118
Identities = 218/542 (40%), Positives = 321/542 (59%), Gaps = 25/542 (4%)
Query: 34 SLSNGHTCVGCVLVVSVIEQLAQVHNSTVQASMERLCSYLPEKLFLKTTCYLVIDKFGSD 93
S + G C C +V+S++ Q + +HN +++ S++ LCS+ P++ + C +++ +G
Sbjct: 47 SANGGTHCAACTIVLSLVNQYSGIHNKSIEDSLDELCSFFPDE--AGSLCTWLVNTYGEQ 104
Query: 94 IIKLLSADMNADVVCHTLEFCKQNTGQPLCHLYPLPKETWKFTLQKARQIVKKSPILKYS 153
II NAD VCH + C P C L FT ++ +Y+
Sbjct: 105 IISYFDQYQNADDVCHAMNVCT----SPTCRL---------FTSAPSKFYNPDHVHQEYN 151
Query: 154 RSGSDICSLPVLAKICQKIKLAMEQSVPFKDVDSDKYSVFPTLRGYHWRGRDCNDSDESV 213
+ + P A I + IK+ + +P +D D D +S+ T RGY+WRG+DCND D+S
Sbjct: 152 KEDPKVGGSP-WAWIQELIKVFSSEHLPIQDFDGDHHSLEATFRGYNWRGKDCNDFDKSS 210
Query: 214 YPGRRPNNWDVHQDSNCNGIWGVDPKDGVPYEKKFCEGSQPRGIILLGDSAGAHFHISPE 273
YPG D D NCNGI+GV+ + YE+ FC S G+I+ GDSAGAHF I P+
Sbjct: 211 YPGTIEGTGDASVDKNCNGIFGVNNQSQ-SYEELFCSNSGAMGVIVSGDSAGAHFSIPPQ 269
Query: 274 WITASQMSLNSFINLPTALTNELDWPQLSGATGFLDSTVGIKEKSIYLRLWKRNHCNHRD 333
W+TA ++ ++ + L E+DWP S TG+ S + S+YLR+ +RN CNHRD
Sbjct: 270 WMTARDINSTTYKGMIDILETEVDWPMRSCYTGWDTSAPNV--DSMYLRMRERNLCNHRD 327
Query: 334 YQNISRNGASSRNLKKFIESLSRNKVLDYPAIVIYAMIGNDVCSGKSDPVPAMTTPEKLY 393
YQN+ NGASS + + I++++RN+ D P ++ +IGNDVCSG D +MTT E+
Sbjct: 328 YQNLGVNGASSSSTRTIIQTINRNQNTDKPVLLFLELIGNDVCSGHHD-YDSMTTVEEFG 386
Query: 394 SNVMQTLKHLNSHLPNGSHVILYGLPDGTFLWDNLHNRYHPLGQLNKDMTYAQLYSFLNC 453
N++ L +L + LP GSHV+ GL DG LWD+L NR HPLG TY ++Y FLNC
Sbjct: 387 ENILTILNYLETTLPKGSHVVFVGLADGRVLWDSLWNRTHPLG-----CTYEEVYDFLNC 441
Query: 454 LQVSPCHGWMSSNKTLRTLTSERAEQLSNTLKKIAASEKFTNFNLFYMDFAFHEIIQEWQ 513
+QVSPC GWM+ N+T+R TS RA QLS ++ + F NF++ Y DF F I + W
Sbjct: 442 IQVSPCWGWMNPNETVRDYTSNRAAQLSLVYNQLIKNYTFNNFDMIYYDFPFATINEMWV 501
Query: 514 KRGGQPWQLIEPVDGFHPNEVALLLLADHFWKKVQLQWPQILGKENPFNPQIKQVFGDQG 573
+GGQ + LIEP+DGFHPN++A L+A F+ ++ P+ LG+ N +N QI+ +FG+QG
Sbjct: 502 AQGGQTYDLIEPIDGFHPNQIANYLMAGVFYNQIAQDKPEWLGEINQYNTQIQSIFGNQG 561
Query: 574 GH 575
G+
Sbjct: 562 GY 563
>emb|CAC50079.1| GPI inositol deacylase [Trypanosoma brucei brucei]
Length = 558
Score = 348 bits (885), Expect = 2e-94
Identities = 203/568 (35%), Positives = 308/568 (53%), Gaps = 44/568 (7%)
Query: 18 SLQSSASPANDDQSRPSLSNGHTCVGCVLVVSVIEQLAQVHNSTVQASMERLCSYL--PE 75
S+ ++ + A + +S S S+ CV C VV+ + QL Q+H + ++ CS+ E
Sbjct: 25 SIHTAKAEARESKSLGS-SDTWGCVLCTAVVATVSQLGQLHQIPAKDALGLFCSFFRSAE 83
Query: 76 KLFLKTTCYLVIDKFGSDIIKLLSADMNADVVCHTLEFCKQNTGQPLCHLYPLPKETWKF 135
+ KT ++++ ++L+ D VC +++C C L+P P +
Sbjct: 84 AVLCKTASLILLE----GALQLIDEGRTPDKVCQIMKYCDTEE----CILFP-PDKNDTL 134
Query: 136 TLQKARQIVKKSPILKYSRSGSDIC-SLPVLAKICQKIKLAMEQSVPFKDVDSDKYSVFP 194
L K R + R D C + P L C+ + E S D D D++S +P
Sbjct: 135 PLHKLRSAYRLD-----GRKLVDACRTFPTL---CRPL---FEDSA--HDKDGDRFSTYP 181
Query: 195 TLRGYHWRGRDCNDSDESVYPGRRPNNWDVHQDSNCNGIWGVDPKDGVPYEKKFCEGSQP 254
RG WRGRDC+D D VYPGR N+ D +D NCNGI+GV+ YE+ +C S P
Sbjct: 182 AKRGTDWRGRDCDDDDARVYPGR--NSTDSQRDENCNGIYGVEESSKKTYEELWCSNSSP 239
Query: 255 RGIILLGDSAGAHFHISPEWITASQMSLNSFINLPTALTNELDWPQLSGATGFLDST--- 311
G+I +GDSA A F I +++ +S + N+ L NE+DWP LSG TGF +ST
Sbjct: 240 MGVIAVGDSATADFGIPLGYVSILDLSSEAVANIIPVLDNEIDWPMLSGLTGFANSTRYE 299
Query: 312 --VGIKEKSIYLRLWKRNHCNHRDYQNISRNGASSRNLKKFIESLSRNKVLDY-PAIVIY 368
V S+Y +L + N CNHRDYQN+ NGA S ++ ++S++RN+ PA V +
Sbjct: 300 PDVDGPMTSVYSKLVELNRCNHRDYQNLGHNGARSGSILSILDSVARNRSESVKPAYVFF 359
Query: 369 AMIGNDVCSGKSDPVPAMTTPEKLYSNVMQTLKHLNSHLPNGSHVILYGLPDGTFLWDNL 428
+M+GNDVC D TP Y ++ + +K +S LP GSHV++ L D ++
Sbjct: 360 SMVGNDVCPRPQDA----PTPLDYYHSLSEAVKKADSFLPPGSHVLIVSLVDARAAIGSI 415
Query: 429 HNRYHPLGQLNKDMTYAQLYSFLNCLQVSPCHGWMSSNKTLRTLTSERAEQLSNTLKKIA 488
NR HP+G LN D+TY LY +LNCLQVSPC GW++SN+++R T ERA +++ + ++
Sbjct: 416 VNRIHPIGTLNSDVTYGNLYDYLNCLQVSPCWGWLNSNESVRNETWERASAMNSMISRVV 475
Query: 489 -ASEKFTNFNLFYMDFAFHEIIQEWQKRGGQPWQLIEPVDGFHPNEVALLLLADHFWKKV 547
S N + + E++ ++ G W+L+ PVDGFHP+++A L+ + K+
Sbjct: 476 DESTNLENVKVHALGDVIGEVVSSFE---GPLWKLVNPVDGFHPSQIATALMGARVFAKM 532
Query: 548 QLQWPQILGKENPFNPQIKQVFGDQGGH 575
Q IL ENPFN I++ FGDQGG+
Sbjct: 533 QDL--GILPTENPFNGDIQRRFGDQGGY 558
>ref|XP_225370.1| similar to acyloxyacyl hydrolase [Mus musculus] [Rattus norvegicus]
Length = 212
Score = 128 bits (320), Expect = 3e-28
Identities = 50/59 (84%), Positives = 57/59 (95%)
Query: 176 MEQSVPFKDVDSDKYSVFPTLRGYHWRGRDCNDSDESVYPGRRPNNWDVHQDSNCNGIW 234
M+++VPFKD DSDKYSVFPTLRGYHWRGRDCND D++VYPGRRP+NWD+HQDSNCNGIW
Sbjct: 1 MKKAVPFKDGDSDKYSVFPTLRGYHWRGRDCNDKDKTVYPGRRPDNWDIHQDSNCNGIW 59
>pir||T00207 P109 protein - silkworm
dbj|BAA23126.1| BmP109 [Bombyx mori]
Length = 965
Score = 48.9 bits (115), Expect = 3e-04
Identities = 25/89 (28%), Positives = 48/89 (53%), Gaps = 4/89 (4%)
Query: 36 SNGHTCVGCVLVVSVIEQLAQVHNS--TVQASMERLCSYLPEKLFLKTTCYLVIDKFGSD 93
S C C+ V +E + + + S ++ +++ LC+ L +K L++ C +D + S
Sbjct: 636 SEKSNCPLCLFAVEQLESVLKNNRSEENIRKALDGLCTRLSQK--LQSECIDFVDTYSSQ 693
Query: 94 IIKLLSADMNADVVCHTLEFCKQNTGQPL 122
++++L ADMNA +C L+ C+ PL
Sbjct: 694 LVEMLVADMNAKEICVFLKLCRDQLHDPL 722
Score = 40.4 bits (93), Expect = 0.12
Identities = 21/82 (25%), Positives = 43/82 (51%), Gaps = 4/82 (4%)
Query: 36 SNGHTCVGCVLVVSVI-EQLAQVHN-STVQASMERLCSYLPEKLFLKTTCYLVIDKFGSD 93
S + CV C V+ I +Q+ HN ++ ++ +C ++P+ + C ++K+
Sbjct: 782 SQSNVCVLCEFVLKEIDDQIKDKHNDDEIKKAVHGICKHMPKS--VSAECDQFVEKYADL 839
Query: 94 IIKLLSADMNADVVCHTLEFCK 115
+I LL+ +++ VC L+ CK
Sbjct: 840 VISLLAQELDPSEVCEELKLCK 861
>sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucosylceramidase activator)
(Sphingolipid activator protein 2) (SAP-2)
pir||A32026 glucosylceramide beta-glucosidase activator protein SAP-2 - guinea
pig
Length = 81
Score = 43.5 bits (101), Expect = 0.014
Identities = 24/78 (30%), Positives = 41/78 (51%), Gaps = 6/78 (7%)
Query: 40 TCVGCVLVVSVIEQLAQVHNSTVQA---SMERLCSYLPEKLFLKTTCYLVIDKFGSDIIK 96
TC C VV + +L +N T + +++ +C+ LPE + C V+D +G I+
Sbjct: 4 TCKACEYVVKKVMELID-NNRTEEKIIHALDSVCALLPES--VSEVCQEVVDTYGDSIVA 60
Query: 97 LLSADMNADVVCHTLEFC 114
LL +M+ ++VC L C
Sbjct: 61 LLLQEMSPELVCSELGLC 78
>ref|XP_223521.1| similar to prosaposin (variant Gaucher disease and variant
metachromatic leukodystrophy); Prosaposin (sphingolipid
activator protein-1) [Homo sapiens] [Rattus norvegicus]
Length = 527
Score = 42.3 bits (98), Expect = 0.031
Identities = 22/79 (27%), Positives = 47/79 (58%), Gaps = 5/79 (6%)
Query: 38 GHTCVGCVLVVSVIEQLAQVHNST--VQASMERLCSYLPEKLFLKTTCYLVIDKFGSDII 95
G TC C+ VV +++ + +++ + ++ER+CS +PE L + C ++D +G +++
Sbjct: 297 GLTCDVCLNVVQELDKWLETNSTEALISHALERVCSIMPESLVQQ--CITLVDTYGPNLV 354
Query: 96 KLLSADMNADVVCHTLEFC 114
+L+S + + VC T+ C
Sbjct: 355 QLVS-KVTPEKVCETIXLC 372
>dbj|BAA95677.1| prosaposin [Bos taurus]
Length = 525
Score = 40.0 bits (92), Expect = 0.15
Identities = 25/97 (25%), Positives = 48/97 (48%), Gaps = 5/97 (5%)
Query: 41 CVGCVLVVSVIEQLAQVHNS--TVQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKLL 98
C C VV + +L + + + +++++CS LP L C V+D +GS I+ +L
Sbjct: 316 CEVCEFVVKEVAKLIDNNRTEEEILHALDKVCSKLPTS--LAEQCQEVVDTYGSSILSIL 373
Query: 99 SADMNADVVCHTLEFCKQNTGQPLCHLYPLPKETWKF 135
+ + ++VC L C + G P + +P++ F
Sbjct: 374 LDEASPELVCSMLHLC-SSRGLPAATVRVMPRKDGGF 409
>ref|NP_524597.1| Saposin-related; Aggregation promoting activity; Drosophila
Aggregation promoting activity; 110kD protein
[Drosophila melanogaster]
gb|AAD38622.1|AF145647_1 BcDNA.GH08312 [Drosophila melanogaster]
gb|AAF57097.1| CG12070-PA [Drosophila melanogaster]
Length = 953
Score = 39.6 bits (91), Expect = 0.21
Identities = 22/84 (26%), Positives = 41/84 (48%), Gaps = 4/84 (4%)
Query: 33 PSLSNGHTCVGCVLVVSVIEQLAQVHNS--TVQASMERLCSYLPEKLFLKTTCYLVIDKF 90
P + C+ C +V +E+ H + +++ +E C + + + T C+ VIDK+
Sbjct: 658 PGFTAAPNCLICEELVKTLEKRMGKHPTRDSIKHILEESCDRMRKP--MNTKCHKVIDKY 715
Query: 91 GSDIIKLLSADMNADVVCHTLEFC 114
G I LL +M+ ++C L C
Sbjct: 716 GDKIADLLLKEMDPKLICTELGMC 739
>ref|NP_733408.1| CG12070-PB [Drosophila melanogaster]
gb|AAN14261.1|AE003775_32 CG12070-PB [Drosophila melanogaster]
Length = 469
Score = 39.6 bits (91), Expect = 0.22
Identities = 22/84 (26%), Positives = 41/84 (48%), Gaps = 4/84 (4%)
Query: 33 PSLSNGHTCVGCVLVVSVIEQLAQVHNS--TVQASMERLCSYLPEKLFLKTTCYLVIDKF 90
P + C+ C +V +E+ H + +++ +E C + + + T C+ VIDK+
Sbjct: 174 PGFTAAPNCLICEELVKTLEKRMGKHPTRDSIKHILEESCDRMRKP--MNTKCHKVIDKY 231
Query: 91 GSDIIKLLSADMNADVVCHTLEFC 114
G I LL +M+ ++C L C
Sbjct: 232 GDKIADLLLKEMDPKLICTELGMC 255
>emb|CAA40392.1| cerebroside sulfate activator [Homo sapiens]
Length = 210
Score = 38.4 bits (88), Expect = 0.52
Identities = 22/77 (28%), Positives = 41/77 (52%), Gaps = 6/77 (7%)
Query: 41 CVGCVLVVSVIEQLAQVHNST---VQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKL 97
C C +V + +L +N T + + +++CS LP+ L C V+D +GS I+ +
Sbjct: 12 CEVCEFLVKEVTKLID-NNKTEKEILDAFDKMCSKLPKS--LSEECQEVVDTYGSSILSI 68
Query: 98 LSADMNADVVCHTLEFC 114
L +++ ++VC L C
Sbjct: 69 LLEEVSPELVCSMLHLC 85
>pir||SAHUP saposin precursor [validated] - human
dbj|BAA00321.1| sphingolipid activator proteins [Homo sapiens]
prf||1504251A sphingolipid activator
Length = 527
Score = 38.4 bits (88), Expect = 0.52
Identities = 22/77 (28%), Positives = 41/77 (52%), Gaps = 6/77 (7%)
Query: 41 CVGCVLVVSVIEQLAQVHNST---VQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKL 97
C C +V + +L +N T + + +++CS LP+ L C V+D +GS I+ +
Sbjct: 318 CEVCEFLVKEVTKLID-NNKTEKEILDAFDKMCSKLPKS--LSEECQEVVDTYGSSILSI 374
Query: 98 LSADMNADVVCHTLEFC 114
L +++ ++VC L C
Sbjct: 375 LLEEVSPELVCSMLHLC 391
>gb|AAA36594.1| cerebroside sulfate activator protein
Length = 527
Score = 38.4 bits (88), Expect = 0.52
Identities = 22/77 (28%), Positives = 41/77 (52%), Gaps = 6/77 (7%)
Query: 41 CVGCVLVVSVIEQLAQVHNST---VQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKL 97
C C +V + +L +N T + + +++CS LP+ L C V+D +GS I+ +
Sbjct: 318 CEVCEFLVKEVTKLID-NNKTEKEILDAFDKMCSKLPKS--LSEECQEVVDTYGSSILSI 374
Query: 98 LSADMNADVVCHTLEFC 114
L +++ ++VC L C
Sbjct: 375 LLEEVSPELVCSMLHLC 391
>ref|NP_002769.1| prosaposin (variant Gaucher disease and variant metachromatic
leukodystrophy); Prosaposin (sphingolipid activator
protein-1) [Homo sapiens]
sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
gb|AAA52560.1| co-beta glucosidase precursor
gb|AAB59494.1| sphingolipid activator precursor
gb|AAA60303.1| prosaposin
gb|AAH04275.1|AAH04275 prosaposin (variant Gaucher disease and variant metachromatic
leukodystrophy) [Homo sapiens]
gb|AAH07612.1|AAH07612 prosaposin (variant Gaucher disease and variant metachromatic
leukodystrophy) [Homo sapiens]
gb|AAH01503.1|AAH01503 prosaposin (variant Gaucher disease and variant metachromatic
leukodystrophy) [Homo sapiens]
Length = 524
Score = 38.4 bits (88), Expect = 0.52
Identities = 22/77 (28%), Positives = 41/77 (52%), Gaps = 6/77 (7%)
Query: 41 CVGCVLVVSVIEQLAQVHNST---VQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKL 97
C C +V + +L +N T + + +++CS LP+ L C V+D +GS I+ +
Sbjct: 315 CEVCEFLVKEVTKLID-NNKTEKEILDAFDKMCSKLPKS--LSEECQEVVDTYGSSILSI 371
Query: 98 LSADMNADVVCHTLEFC 114
L +++ ++VC L C
Sbjct: 372 LLEEVSPELVCSMLHLC 388
>gb|AAA36595.1| cerebroside sulfate activator protein
Length = 524
Score = 38.4 bits (88), Expect = 0.53
Identities = 22/77 (28%), Positives = 41/77 (52%), Gaps = 6/77 (7%)
Query: 41 CVGCVLVVSVIEQLAQVHNST---VQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKL 97
C C +V + +L +N T + + +++CS LP+ L C V+D +GS I+ +
Sbjct: 315 CEVCEFLVKEVTKLID-NNKTEKEILDAFDKMCSKLPKS--LSEECQEVVDTYGSSILSI 371
Query: 98 LSADMNADVVCHTLEFC 114
L +++ ++VC L C
Sbjct: 372 LLEEVSPELVCSMLHLC 388
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 525
Score = 38.4 bits (88), Expect = 0.55
Identities = 24/97 (24%), Positives = 47/97 (47%), Gaps = 5/97 (5%)
Query: 41 CVGCVLVVSVIEQLAQVHNS--TVQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKLL 98
C C VV + +L + + + +++++CS LP L C V+D +G I+ +L
Sbjct: 316 CEVCEFVVKEVAKLIDNNRTEEEILHALDKVCSKLPTS--LAEQCQEVVDTYGRSILSIL 373
Query: 99 SADMNADVVCHTLEFCKQNTGQPLCHLYPLPKETWKF 135
+ + ++VC L C + G P + +P++ F
Sbjct: 374 LDEASPELVCSMLHLC-SSRGLPAATVRVMPRKDGGF 409
>gb|AAA36596.1| cerebroside sulfate activator protein
Length = 526
Score = 38.0 bits (87), Expect = 0.58
Identities = 22/77 (28%), Positives = 41/77 (52%), Gaps = 6/77 (7%)
Query: 41 CVGCVLVVSVIEQLAQVHNST---VQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKL 97
C C +V + +L +N T + + +++CS LP+ L C V+D +GS I+ +
Sbjct: 317 CEVCEFLVKEVTKLID-NNKTEKEILDAFDKMCSKLPKS--LSEECQEVVDTYGSSILSI 373
Query: 98 LSADMNADVVCHTLEFC 114
L +++ ++VC L C
Sbjct: 374 LLEEVSPELVCSMLHLC 390
>ref|NP_491803.1| Putative secreted or extracellular protein precursor (13.4 kD)
[Caenorhabditis elegans]
gb|AAK21363.1| Hypothetical protein C48E7.10 [Caenorhabditis elegans]
Length = 118
Score = 38.0 bits (87), Expect = 0.67
Identities = 31/112 (27%), Positives = 51/112 (44%), Gaps = 14/112 (12%)
Query: 7 ILTVAPLFLLLSLQSSASPANDDQSRPSLSNGHTCVGCVLVVSVIEQLAQV--HNSTVQA 64
+L + L +S A + D S+P C L V+V++QL QV H ++A
Sbjct: 6 LLAFLAITLTVSNVEGAKKMHSDTSKPL---------CGLCVNVVKQLDQVLEHGGDIEA 56
Query: 65 SMERLCSY-LPEKLFLKTTCYLVIDKFGSDIIKLLSADMNADVVCHTLEFCK 115
++++ C +P F+ C VI+K II L AD +C + C+
Sbjct: 57 AVDKFCKEDVPS--FMVDMCEKVIEKNLEYIINKLKDHEEADKICTDILLCR 106
>gb|AAL92313.1| Proteasome subunit beta type 1 [Dictyostelium discoideum]
Length = 200
Score = 37.3 bits (85), Expect = 1.1
Identities = 22/84 (26%), Positives = 38/84 (45%), Gaps = 4/84 (4%)
Query: 37 NGHTCVGCVLVVSVIEQLAQVH--NSTVQASMERLCSYLPEKLFLKTTCYLVIDKFGSDI 94
N TC C V E L + S + ++ CS LP + + C ++++ +G +
Sbjct: 19 NALTCELCQFSVKAAEDLVTQNFTQSQIIPYLDSACSLLPNQ--WASNCEIIVNTYGLSM 76
Query: 95 IKLLSADMNADVVCHTLEFCKQNT 118
IKL+ + +V C L C N+
Sbjct: 77 IKLVLENETPEVACTQLGLCGSNS 100
>pir||S21770 saposin-C - bovine
gb|AAB21926.1| saposin-C, co-glucosidase, sphingolipid activator protein A,
SAP-2=activator protein [cattle, spleen, Peptide, 80 aa]
Length = 80
Score = 37.3 bits (85), Expect = 1.2
Identities = 22/77 (28%), Positives = 39/77 (50%), Gaps = 6/77 (7%)
Query: 41 CVGCVLVVSVIEQLAQVHNST---VQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKL 97
C C VV + +L +N T + +++++CS LP L C V+D +G I+ +
Sbjct: 5 CQVCEFVVKEVAKLID-NNRTEEEILHALDKVCSKLPTS--LAEQCQEVVDTYGRSILSI 61
Query: 98 LSADMNADVVCHTLEFC 114
L + + ++VC L C
Sbjct: 62 LLDEASPELVCSMLHLC 78
>gb|AAL96718.1| COUNTIN [Dictyostelium discoideum]
Length = 258
Score = 36.9 bits (84), Expect = 1.6
Identities = 30/88 (34%), Positives = 45/88 (51%), Gaps = 6/88 (6%)
Query: 35 LSNGHTCVGCVLVV--SVIEQLAQVHNSTVQASMERLCSYLPEKLFLKTTCYLVIDKFGS 92
+S+ +C CV V S+ + L + NS V + LCS +P + T C L+ D G
Sbjct: 19 VSSLDSCSICVDFVGNSLNDLLNIILNSGVIGTCGDLCSAVPGGQIVDTVCDLLCDYVGV 78
Query: 93 D-IIKLLSADMNAD--VVCHTLEFCKQN 117
D IKL+S D++ D +C + CK N
Sbjct: 79 DEFIKLIS-DVDPDPIYICEKISVCKTN 105
>gb|AAK77913.1|AF400074_2 surfactant, pulmonary-associated protein B [Homo sapiens]
Length = 381
Score = 36.1 bits (82), Expect = 2.4
Identities = 21/83 (25%), Positives = 42/83 (50%), Gaps = 3/83 (3%)
Query: 41 CVGCVLVVSVIEQLAQ--VHNSTVQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKLL 98
C C +V ++ ++A+ + T++ +E+ C+ LP KL L C V+D + +I
Sbjct: 69 CQECEDIVHILNKMAKEAIFQDTMRKFLEQECNVLPLKL-LMPQCNQVLDDYFPLVIDYF 127
Query: 99 SADMNADVVCHTLEFCKQNTGQP 121
++++ +C L CK +P
Sbjct: 128 QNQIDSNGICMHLGLCKSRQPEP 150
>ref|NP_000533.1| surfactant, pulmonary-associated protein B; Pulmonary
surfactant-associated protein B, 18kD [Homo sapiens]
gb|AAB59541.1| pulmonary surfactant-associated protein SP-B
Length = 381
Score = 36.1 bits (82), Expect = 2.4
Identities = 21/83 (25%), Positives = 42/83 (50%), Gaps = 3/83 (3%)
Query: 41 CVGCVLVVSVIEQLAQ--VHNSTVQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKLL 98
C C +V ++ ++A+ + T++ +E+ C+ LP KL L C V+D + +I
Sbjct: 69 CQECEDIVHILNKMAKEAIFQDTMRKFLEQECNVLPLKL-LMPQCNQVLDDYFPLVIDYF 127
Query: 99 SADMNADVVCHTLEFCKQNTGQP 121
++++ +C L CK +P
Sbjct: 128 QNQIDSNGICMHLGLCKSRQPEP 150
CPU time: 140.50 user secs. 2.54 sys. secs 143.04 total secs.
Database: nr
Posted date: Jan 18, 2003 1:23 PM
Number of letters in database: 421,297,171
Number of sequences in database: 1,315,992
Lambda K H
0.319 0.136 0.427
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 511394837
Number of Sequences: 1315992
Number of extensions: 22631742
Number of successful extensions: 46736
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 42
Number of HSP's that attempted gapping in prelim test: 46679
Number of HSP's gapped (non-prelim): 81
length of query: 575
length of database: 421,297,171
effective HSP length: 56
effective length of query: 519
effective length of database: 347,601,619
effective search space: 180405240261
effective search space used: 180405240261
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 77 (34.4 bits)
