Sequences with E-value BETTER than threshold (0.002) 1182 0.0
444 e-123
430 e-119
429 e-119
328 3e-88
66 3e-09
54 1e-05
53 2e-05
47 0.002
Sequences with E-value WORSE than threshold sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Conta... 45 0.005
dbj|BAA95677.1| prosaposin [Bos taurus] 45 0.005
emb|CAA40392.1| cerebroside sulfate activator [Homo sapiens] 42 0.047
pir||S21770 saposin-C - bovine >gi|248087|gb|AAB21926.1| sapo... 42 0.053
gb|AAB06759.1| SapA 41 0.080
pir||SAHUP saposin precursor [validated] - human >gi|220064|d... 41 0.098
gb|AAA36594.1| cerebroside sulfate activator protein 41 0.098
gb|AAA36596.1| cerebroside sulfate activator protein 41 0.098
ref|NP_002769.1| prosaposin (variant Gaucher disease and vari... 41 0.10
gb|AAA36595.1| cerebroside sulfate activator protein 41 0.10
ref|NP_524597.1| Saposin-related; Aggregation promoting activ... 40 0.12
gb|AAD45802.1|AF162129_1 ecdysone dependent glycoprotein [Dro... 40 0.13
pir||T00207 P109 protein - silkworm >gi|2575865|dbj|BAA23126.... 38 0.71
ref|XP_192668.1| similar to Sulfated glycoprotein 1 precursor... 38 0.76
dbj|BAC40308.1| unnamed protein product [Mus musculus] >gi|26... 38 0.76
ref|NP_035309.1| prosaposin [Mus musculus] >gi|1381582|gb|AAB... 38 0.82
Alignments
>gb|AAM43641.1| ACYLOXYACYL HYDROLASE [Dictyostelium discoideum]
Length = 564
Score = 1182 bits (3026), Expect = 0.0
Identities = 564/564 (100%), Positives = 564/564 (100%)
Query: 1 MKSVFVLLFIVLSVLINFTTAVNIPVNGKSLEIKNKFEEFISNDVKSANGGTHCAACTIV 60
MKSVFVLLFIVLSVLINFTTAVNIPVNGKSLEIKNKFEEFISNDVKSANGGTHCAACTIV
Sbjct: 1 MKSVFVLLFIVLSVLINFTTAVNIPVNGKSLEIKNKFEEFISNDVKSANGGTHCAACTIV 60
Query: 61 LSLVNQYSGIHNKSIEDSLDELCSFFPDEAGSLCTWLVNTYGEQIISYFDQYQNADDVCH 120
LSLVNQYSGIHNKSIEDSLDELCSFFPDEAGSLCTWLVNTYGEQIISYFDQYQNADDVCH
Sbjct: 61 LSLVNQYSGIHNKSIEDSLDELCSFFPDEAGSLCTWLVNTYGEQIISYFDQYQNADDVCH 120
Query: 121 AMNVCTSPTCRLFTSAPSKFYNPDHVHQEYNKEDPKVGGSPWAWIQELIKVFSSEHLPIQ 180
AMNVCTSPTCRLFTSAPSKFYNPDHVHQEYNKEDPKVGGSPWAWIQELIKVFSSEHLPIQ
Sbjct: 121 AMNVCTSPTCRLFTSAPSKFYNPDHVHQEYNKEDPKVGGSPWAWIQELIKVFSSEHLPIQ 180
Query: 181 DFDGDHHSLEATFRGYNWRGKDCNDFDKSSYPGTIEGTGDASVDKNCNGIFGVNNQSQSY 240
DFDGDHHSLEATFRGYNWRGKDCNDFDKSSYPGTIEGTGDASVDKNCNGIFGVNNQSQSY
Sbjct: 181 DFDGDHHSLEATFRGYNWRGKDCNDFDKSSYPGTIEGTGDASVDKNCNGIFGVNNQSQSY 240
Query: 241 EELFCSNSGAMGVIVSGDSAGAHFSIPPQWMTARDINSTTYKGMIDILETEVDWPMRSCY 300
EELFCSNSGAMGVIVSGDSAGAHFSIPPQWMTARDINSTTYKGMIDILETEVDWPMRSCY
Sbjct: 241 EELFCSNSGAMGVIVSGDSAGAHFSIPPQWMTARDINSTTYKGMIDILETEVDWPMRSCY 300
Query: 301 TGWDTSAPNVDSMYLRMRERNLCNHRDYQNLGVNGASSSSTRTIIQTINRNQNTDKPVLL 360
TGWDTSAPNVDSMYLRMRERNLCNHRDYQNLGVNGASSSSTRTIIQTINRNQNTDKPVLL
Sbjct: 301 TGWDTSAPNVDSMYLRMRERNLCNHRDYQNLGVNGASSSSTRTIIQTINRNQNTDKPVLL 360
Query: 361 FLELIGNDVCSGHHDYDSMTTVEEFGENILTILNYLETTLPKGSHVVFVGLADGRVLWDS 420
FLELIGNDVCSGHHDYDSMTTVEEFGENILTILNYLETTLPKGSHVVFVGLADGRVLWDS
Sbjct: 361 FLELIGNDVCSGHHDYDSMTTVEEFGENILTILNYLETTLPKGSHVVFVGLADGRVLWDS 420
Query: 421 LWNRTHPLGCTYEEVYDFLNCIQVSPCWGWMNPNETVRDYTSNRAAQLSLVYNQLIKNYT 480
LWNRTHPLGCTYEEVYDFLNCIQVSPCWGWMNPNETVRDYTSNRAAQLSLVYNQLIKNYT
Sbjct: 421 LWNRTHPLGCTYEEVYDFLNCIQVSPCWGWMNPNETVRDYTSNRAAQLSLVYNQLIKNYT 480
Query: 481 FNNFDMIYYDFPFATINEMWVAQGGQTYDLIEPIDGFHPNQIANYLMAGVFYNQIAQDKP 540
FNNFDMIYYDFPFATINEMWVAQGGQTYDLIEPIDGFHPNQIANYLMAGVFYNQIAQDKP
Sbjct: 481 FNNFDMIYYDFPFATINEMWVAQGGQTYDLIEPIDGFHPNQIANYLMAGVFYNQIAQDKP 540
Query: 541 EWLGEINQYNTQIQSIFGNQGGYV 564
EWLGEINQYNTQIQSIFGNQGGYV
Sbjct: 541 EWLGEINQYNTQIQSIFGNQGGYV 564
>gb|AAB81183.1| acyloxyacyl hydrolase [Oryctolagus cuniculus]
Length = 575
Score = 444 bits (1131), Expect = e-123
Identities = 220/536 (41%), Positives = 323/536 (60%), Gaps = 26/536 (4%)
Query: 54 CAACTIVLSLVNQYSGIHNKSIEDSLDELCSFFPDE--AGSLCTWLVNTYGEQIISYFDQ 111
C C +V+S++ Q + +HN +++ S++ LCS+ P+E + C +V+ +G II FD+
Sbjct: 40 CVGCVLVVSVIEQLAQVHNSTVQASMERLCSYLPEEWVLKTACYMMVHVFGADIIKLFDK 99
Query: 112 YQNADDVCHAMNVCT----SPTCRL---------FTSAPSKFYNPDHVHQEYNKEDPKVG 158
NAD VCH + C P C L FT ++ +Y + +
Sbjct: 100 DVNADVVCHTLEFCKQEPGQPLCHLYPLPKESWKFTLEKARHIVKQSPIMKYTRSGAGIC 159
Query: 159 GSPW-AWIQELIKVFSSEHLPIQDFDGDHHSLEATFRGYNWRGKDCNDFDKSSYPGTIEG 217
P+ A I + IK+ +PI+D D D +S+ T RGY+WRG+DCND DK+ YPG
Sbjct: 160 SLPFLAKICQKIKLAIKNSVPIKDVDSDKYSIFPTLRGYHWRGRDCNDSDKTVYPGRRPD 219
Query: 218 TGDASVDKNCNGIFGVNNQSQ-SYEELFCSNSGAMGVIVSGDSAGAHFSIPPQWMTARDI 276
DA D NCNGI+GV+ + YE+ FC S G+I+ GDSAGAHF IPP+W+T +
Sbjct: 220 NWDAHRDSNCNGIWGVDPKDGIPYEKKFCEGSQPRGIILLGDSAGAHFHIPPEWLTVSQM 279
Query: 277 NSTTYKGMIDILETEVDWPMRSCYTGWDTSAPNV--DSMYLRMRERNLCNHRDYQNLGVN 334
+ ++ + + E+DWP S TG+ SA + +S+YLR+R+RN CNHRDYQN+ N
Sbjct: 280 SVNSFLNLPTAVTNELDWPQLSGTTGFLDSASKIKENSIYLRLRKRNRCNHRDYQNISKN 339
Query: 335 GASSSSTRTIIQTINRNQNTDKPVLLFLELIGNDVCSGHHD--YDSMTTVEEFGENILTI 392
GASS + +++I++++RNQ D P ++ +IGNDVC+G +MTT E+ N+L +
Sbjct: 340 GASSRNVKSLIESLSRNQLLDHPAIVIYAMIGNDVCNGRKTDPVSAMTTPEQLYANVLKM 399
Query: 393 LNYLETTLPKGSHVVFVGLADGRVLWDSLWNRTHPLG-----CTYEEVYDFLNCIQVSPC 447
L L + LP GSHV+ GLA G LWD+L +R HPLG TY ++Y FL C+QVSPC
Sbjct: 400 LEALNSHLPTGSHVILYGLAHGAFLWDTLHSRYHPLGQLNKDVTYTQLYSFLGCLQVSPC 459
Query: 448 WGWMNPNETVRDYTSNRAAQLSLVYNQLIKNYTFNNFDMIYYDFPFATINEMWVAQGGQT 507
GWM+ NET+R TS RA QLS ++ + F NF++ Y DF F + E W GGQ
Sbjct: 460 PGWMSANETLRALTSERAQQLSETLRKIAASKKFTNFNLFYLDFAFQEVVEEWQKMGGQP 519
Query: 508 YDLIEPIDGFHPNQIANYLMAGVFYNQIAQDKPEWLGEINQYNTQIQSIFGNQGGY 563
++LIE +DGFHPN++A L A + ++ + P+ LG+ N +N QI+ +FG+QGG+
Sbjct: 520 WELIEAVDGFHPNEVALLLFADQLWEKVQRQWPDVLGKENPFNPQIEEVFGDQGGH 575
>ref|NP_036184.1| acyloxyacyl hydrolase [Mus musculus]
gb|AAB81182.1| acyloxyacyl hydrolase [Mus musculus]
dbj|BAC39187.1| unnamed protein product [Mus musculus]
Length = 574
Score = 430 bits (1094), Expect = e-119
Identities = 217/543 (39%), Positives = 322/543 (58%), Gaps = 27/543 (4%)
Query: 47 SANGGTHCAACTIVLSLVNQYSGIHNKSIEDSLDELCSFFPDE--AGSLCTWLVNTYGEQ 104
S + G C C +++S++ Q + +HN S++ +++ LCS+ P++ + C +LV T+G
Sbjct: 33 SYSHGQSCLGCVVLVSVIEQLAEVHNSSVQVAMERLCSYLPEKLFLKTACYFLVQTFGSD 92
Query: 105 IISYFDQYQNADDVCHAMNVC----TSPTCRLFTSAPSKFYNP--DHVHQ--------EY 150
II D+ AD VC+A+ C P C L+ P + + + Q +Y
Sbjct: 93 IIKLLDEAMKADVVCYALEFCKRGAVQPQCHLY-PLPQEAWESALEKARQVLRRSSTMKY 151
Query: 151 NKEDPKVGGSPW-AWIQELIKVFSSEHLPIQDFDGDHHSLEATFRGYNWRGKDCNDFDKS 209
+ + P+ I + I++ + +P +D D D HS+ T RGY+WRG+DCND DK+
Sbjct: 152 PRSGRNICSLPFLTKICQKIELSIKKAVPFKDIDSDKHSVFPTLRGYHWRGRDCNDSDKT 211
Query: 210 SYPGTIEGTGDASVDKNCNGIFGVNNQSQ-SYEELFCSNSGAMGVIVSGDSAGAHFSIPP 268
YPG D D NCNGI+G++ + YE+ FC S G+I+ GDSAGAHF IPP
Sbjct: 212 VYPGRRPDNWDIHQDSNCNGIWGIDPKDGIPYEKKFCEGSQPRGIILLGDSAGAHFHIPP 271
Query: 269 QWMTARDINSTTYKGMIDILETEVDWPMRSCYTGW--DTSAPNVDSMYLRMRERNLCNHR 326
+W+TA ++ ++ + L E++WP S TG+ TS S+Y R+R+RN CNHR
Sbjct: 272 EWLTASQMSVNSFLNLPSALTDELNWPQLSGVTGFLDSTSGIEEKSIYHRLRKRNHCNHR 331
Query: 327 DYQNLGVNGASSSSTRTIIQTINRNQNTDKPVLLFLELIGNDVCSGHHD-YDSMTTVEEF 385
DYQ++ NGASS + + I++++RNQ +D P ++ +IGNDVC+ D MTT E+
Sbjct: 332 DYQSISKNGASSRNLKNFIESLSRNQASDHPAIVLYAMIGNDVCNSKADTVPEMTTPEQM 391
Query: 386 GENILTILNYLETTLPKGSHVVFVGLADGRVLWDSLWNRTHPLG-----CTYEEVYDFLN 440
N++ L +L + LP GSHV+ GL DG LWDSL NR HPLG TY + + FL
Sbjct: 392 YANVMQTLTHLNSHLPNGSHVILYGLPDGTFLWDSLHNRYHPLGQLNKDVTYAQFFSFLR 451
Query: 441 CIQVSPCWGWMNPNETVRDYTSNRAAQLSLVYNQLIKNYTFNNFDMIYYDFPFATINEMW 500
C+Q++PC GWM+ N+T+R TS RA QLS ++ TF NFD+ Y DF F I E W
Sbjct: 452 CLQLNPCNGWMSSNKTLRTLTSERAEQLSNTLKKIATTETFANFDLFYVDFAFHEIIEDW 511
Query: 501 VAQGGQTYDLIEPIDGFHPNQIANYLMAGVFYNQIAQDKPEWLGEINQYNTQIQSIFGNQ 560
+GGQ + LIEP+DGFHPN++A+ L A + +I P LG+ N +N+QI+ +FG+Q
Sbjct: 512 QKRGGQPWQLIEPVDGFHPNEVASLLQANRVWEKIQLQWPHVLGKENPFNSQIEEVFGDQ 571
Query: 561 GGY 563
GG+
Sbjct: 572 GGH 574
>ref|NP_001628.1| acyloxyacyl hydrolase precursor [Homo sapiens]
sp|P28039|AOAH_HUMAN Acyloxyacyl hydrolase precursor
pir||A40292 acyloxyacyl hydrolase (EC 3.1.1.-), neutrophil precursor
[validated] - human
gb|AAA35506.1| acyloxyacyl hydrolase
gb|AAH25698.1| acyloxyacyl hydrolase (neutrophil) [Homo sapiens]
Length = 575
Score = 429 bits (1092), Expect = e-119
Identities = 218/542 (40%), Positives = 321/542 (59%), Gaps = 25/542 (4%)
Query: 47 SANGGTHCAACTIVLSLVNQYSGIHNKSIEDSLDELCSFFPDE--AGSLCTWLVNTYGEQ 104
S + G C C +V+S++ Q + +HN +++ S++ LCS+ P++ + C +++ +G
Sbjct: 34 SLSNGHTCVGCVLVVSVIEQLAQVHNSTVQASMERLCSYLPEKLFLKTTCYLVIDKFGSD 93
Query: 105 IISYFDQYQNADDVCHAMNVCT----SPTCRL---------FTSAPSKFYNPDHVHQEYN 151
II NAD VCH + C P C L FT ++ +Y+
Sbjct: 94 IIKLLSADMNADVVCHTLEFCKQNTGQPLCHLYPLPKETWKFTLQKARQIVKKSPILKYS 153
Query: 152 KEDPKVGGSP-WAWIQELIKVFSSEHLPIQDFDGDHHSLEATFRGYNWRGKDCNDFDKSS 210
+ + P A I + IK+ + +P +D D D +S+ T RGY+WRG+DCND D+S
Sbjct: 154 RSGSDICSLPVLAKICQKIKLAMEQSVPFKDVDSDKYSVFPTLRGYHWRGRDCNDSDESV 213
Query: 211 YPGTIEGTGDASVDKNCNGIFGVNNQSQ-SYEELFCSNSGAMGVIVSGDSAGAHFSIPPQ 269
YPG D D NCNGI+GV+ + YE+ FC S G+I+ GDSAGAHF I P+
Sbjct: 214 YPGRRPNNWDVHQDSNCNGIWGVDPKDGVPYEKKFCEGSQPRGIILLGDSAGAHFHISPE 273
Query: 270 WMTARDINSTTYKGMIDILETEVDWPMRSCYTGWDTSAPNV--DSMYLRMRERNLCNHRD 327
W+TA ++ ++ + L E+DWP S TG+ S + S+YLR+ +RN CNHRD
Sbjct: 274 WITASQMSLNSFINLPTALTNELDWPQLSGATGFLDSTVGIKEKSIYLRLWKRNHCNHRD 333
Query: 328 YQNLGVNGASSSSTRTIIQTINRNQNTDKPVLLFLELIGNDVCSGHHD-YDSMTTVEEFG 386
YQN+ NGASS + + I++++RN+ D P ++ +IGNDVCSG D +MTT E+
Sbjct: 334 YQNISRNGASSRNLKKFIESLSRNKVLDYPAIVIYAMIGNDVCSGKSDPVPAMTTPEKLY 393
Query: 387 ENILTILNYLETTLPKGSHVVFVGLADGRVLWDSLWNRTHPLG-----CTYEEVYDFLNC 441
N++ L +L + LP GSHV+ GL DG LWD+L NR HPLG TY ++Y FLNC
Sbjct: 394 SNVMQTLKHLNSHLPNGSHVILYGLPDGTFLWDNLHNRYHPLGQLNKDMTYAQLYSFLNC 453
Query: 442 IQVSPCWGWMNPNETVRDYTSNRAAQLSLVYNQLIKNYTFNNFDMIYYDFPFATINEMWV 501
+QVSPC GWM+ N+T+R TS RA QLS ++ + F NF++ Y DF F I + W
Sbjct: 454 LQVSPCHGWMSSNKTLRTLTSERAEQLSNTLKKIAASEKFTNFNLFYMDFAFHEIIQEWQ 513
Query: 502 AQGGQTYDLIEPIDGFHPNQIANYLMAGVFYNQIAQDKPEWLGEINQYNTQIQSIFGNQG 561
+GGQ + LIEP+DGFHPN++A L+A F+ ++ P+ LG+ N +N QI+ +FG+QG
Sbjct: 514 KRGGQPWQLIEPVDGFHPNEVALLLLADHFWKKVQLQWPQILGKENPFNPQIKQVFGDQG 573
Query: 562 GY 563
G+
Sbjct: 574 GH 575
>emb|CAC50079.1| GPI inositol deacylase [Trypanosoma brucei brucei]
Length = 558
Score = 328 bits (832), Expect = 3e-88
Identities = 194/532 (36%), Positives = 283/532 (52%), Gaps = 42/532 (7%)
Query: 54 CAACTIVLSLVNQYSGIHNKSIEDSLDELCSFFPDEAGSLCTWLVNTYGEQIISYFDQYQ 113
C CT V++ V+Q +H +D+L CSFF LC E + D+ +
Sbjct: 47 CVLCTAVVATVSQLGQLHQIPAKDALGLFCSFFRSAEAVLCKTASLILLEGALQLIDEGR 106
Query: 114 NADDVCHAMNVCTSPTCRLFTSAPSKFYNPDHVHQEYNKEDPKVGGSPWAWIQELIKVFS 173
D VC M C + C LF + + Y + K + + + F
Sbjct: 107 TPDKVCQIMKYCDTEECILFPPDKNDTLPLHKLRSAYRLDGRK--------LVDACRTFP 158
Query: 174 SEHLPI-----QDFDGDHHSLEATFRGYNWRGKDCNDFDKSSYPGTIEGTGDASVDKNCN 228
+ P+ D DGD S RG +WRG+DC+D D YPG + D+ D+NCN
Sbjct: 159 TLCRPLFEDSAHDKDGDRFSTYPAKRGTDWRGRDCDDDDARVYPG--RNSTDSQRDENCN 216
Query: 229 GIFGVNNQS-QSYEELFCSNSGAMGVIVSGDSAGAHFSIPPQWMTARDINSTTYKGMIDI 287
GI+GV S ++YEEL+CSNS MGVI GDSA A F IP +++ D++S +I +
Sbjct: 217 GIYGVEESSKKTYEELWCSNSSPMGVIAVGDSATADFGIPLGYVSILDLSSEAVANIIPV 276
Query: 288 LETEVDWPMRSCYTGWDTSA---PNVD----SMYLRMRERNLCNHRDYQNLGVNGASSSS 340
L+ E+DWPM S TG+ S P+VD S+Y ++ E N CNHRDYQNLG NGA S S
Sbjct: 277 LDNEIDWPMLSGLTGFANSTRYEPDVDGPMTSVYSKLVELNRCNHRDYQNLGHNGARSGS 336
Query: 341 TRTIIQTINRNQNTD-KPVLLFLELIGNDVCSGHHDYDSMTTVEEFGENILTILNYLETT 399
+I+ ++ RN++ KP +F ++GNDVC D T ++ ++ + ++
Sbjct: 337 ILSILDSVARNRSESVKPAYVFFSMVGNDVCPRPQD---APTPLDYYHSLSEAVKKADSF 393
Query: 400 LPKGSHVVFVGLADGRVLWDSLWNRTHPLG-----CTYEEVYDFLNCIQVSPCWGWMNPN 454
LP GSHV+ V L D R S+ NR HP+G TY +YD+LNC+QVSPCWGW+N N
Sbjct: 394 LPPGSHVLIVSLVDARAAIGSIVNRIHPIGTLNSDVTYGNLYDYLNCLQVSPCWGWLNSN 453
Query: 455 ETVRDYTSNRAAQLSLVYNQLIKNYTFNNFDMIYYDFPFATINEMWVAQGGQTYDLIEPI 514
E+VR+ T RA+ ++ + ++++ T N + + I E+ + G + L+ P+
Sbjct: 454 ESVRNETWERASAMNSMISRVVDEST--NLENVKVHALGDVIGEVVSSFEGPLWKLVNPV 511
Query: 515 DGFHPNQIANYLMAGVFYNQIAQDKPEWLGEI---NQYNTQIQSIFGNQGGY 563
DGFHP+QIA LM + ++ QD LG + N +N IQ FG+QGGY
Sbjct: 512 DGFHPSQIATALMGARVFAKM-QD----LGILPTENPFNGDIQRRFGDQGGY 558
>ref|XP_225370.1| similar to acyloxyacyl hydrolase [Mus musculus] [Rattus norvegicus]
Length = 212
Score = 65.7 bits (158), Expect = 3e-09
Identities = 28/55 (50%), Positives = 36/55 (64%)
Query: 177 LPIQDFDGDHHSLEATFRGYNWRGKDCNDFDKSSYPGTIEGTGDASVDKNCNGIF 231
+P +D D D +S+ T RGY+WRG+DCND DK+ YPG D D NCNGI+
Sbjct: 5 VPFKDGDSDKYSVFPTLRGYHWRGRDCNDKDKTVYPGRRPDNWDIHQDSNCNGIW 59
>gb|AAK21659.1|AF154047_1 naegleriapore B pore-forming peptide precursor [Naegleria fowleri]
Length = 484
Score = 53.6 bits (127), Expect = 1e-05
Identities = 31/84 (36%), Positives = 48/84 (56%), Gaps = 4/84 (4%)
Query: 48 ANGGTHCAACTIVLSLVNQYSGIHN--KSIEDSLDELCS-FFPDEAGSLCTWLVNTYGEQ 104
A GT C AC V+S+ + + +N +S++++LD++C F P S C LVN Y Q
Sbjct: 387 AADGTMCLACEYVISVADNWLIANNTQQSVKNTLDKVCQEFVPSIYQSQCIALVNQYEAQ 446
Query: 105 IISYFD-QYQNADDVCHAMNVCTS 127
+I F+ + N VC A+ VC+S
Sbjct: 447 LIQLFESKVFNPQTVCKAIGVCSS 470
Score = 39.6 bits (91), Expect = 0.20
Identities = 21/84 (25%), Positives = 44/84 (52%), Gaps = 3/84 (3%)
Query: 46 KSANGGTHCAACTIVLSLVNQYSGIHNK--SIEDSLDELCSFFPDEAGSLCTWLVNTYGE 103
K+ N G C C ++++ V + ++ ++E L+++CS P + +LCT+ V Y
Sbjct: 112 KAENSGI-CNMCQLLVTQVENWVESNDTIMTLEKKLEQVCSVIPGQYSALCTYAVEQYLP 170
Query: 104 QIISYFDQYQNADDVCHAMNVCTS 127
I ++ A +C +++C+S
Sbjct: 171 IFIHQVEKQFPALTICQDVHLCSS 194
>gb|AAL01158.1|AF196309_1 naegleriapore B precursor [Naegleria fowleri]
Length = 458
Score = 53.2 bits (126), Expect = 2e-05
Identities = 31/84 (36%), Positives = 48/84 (56%), Gaps = 4/84 (4%)
Query: 48 ANGGTHCAACTIVLSLVNQYSGIHN--KSIEDSLDELCS-FFPDEAGSLCTWLVNTYGEQ 104
A GT C AC V+S+ + + +N +S++++LD++C F P S C LVN Y Q
Sbjct: 361 AADGTMCLACEYVISVADNWLIANNTQQSVKNTLDKVCQEFVPSIYQSQCIALVNQYEAQ 420
Query: 105 IISYFD-QYQNADDVCHAMNVCTS 127
+I F+ + N VC A+ VC+S
Sbjct: 421 LIQLFESKVFNPQTVCKAIGVCSS 444
Score = 39.6 bits (91), Expect = 0.23
Identities = 21/84 (25%), Positives = 44/84 (52%), Gaps = 3/84 (3%)
Query: 46 KSANGGTHCAACTIVLSLVNQYSGIHNK--SIEDSLDELCSFFPDEAGSLCTWLVNTYGE 103
K+ N G C C ++++ V + ++ ++E L+++CS P + +LCT+ V Y
Sbjct: 86 KAENSGI-CNMCQLLVTQVENWVESNDTIMTLEKKLEQVCSVIPGQYSALCTYAVEQYLP 144
Query: 104 QIISYFDQYQNADDVCHAMNVCTS 127
I ++ A +C +++C+S
Sbjct: 145 IFIHQVEKQFPALTICQDVHLCSS 168
>sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucosylceramidase activator)
(Sphingolipid activator protein 2) (SAP-2)
pir||A32026 glucosylceramide beta-glucosidase activator protein SAP-2 - guinea
pig
Length = 81
Score = 46.6 bits (109), Expect = 0.002
Identities = 23/78 (29%), Positives = 40/78 (50%), Gaps = 6/78 (7%)
Query: 54 CAACTIVLSLVNQYSGIHNKSIED----SLDELCSFFPDEAGSLCTWLVNTYGEQIISYF 109
C AC V+ V + I N E+ +LD +C+ P+ +C +V+TYG+ I++
Sbjct: 5 CKACEYVVKKVMEL--IDNNRTEEKIIHALDSVCALLPESVSEVCQEVVDTYGDSIVALL 62
Query: 110 DQYQNADDVCHAMNVCTS 127
Q + + VC + +C S
Sbjct: 63 LQEMSPELVCSELGLCMS 80
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 525
Score = 45.0 bits (105), Expect = 0.005
Identities = 27/93 (29%), Positives = 43/93 (46%), Gaps = 6/93 (6%)
Query: 39 EFISNDVKSANGGTHCAACTIVLSLVNQYSGIHNKSIED----SLDELCSFFPDEAGSLC 94
E I D A +C C V+ V + I N E+ +LD++CS P C
Sbjct: 301 EPIKKDPAPAKADIYCEVCEFVVKEVAKL--IDNNRTEEEILHALDKVCSKLPTSLAEQC 358
Query: 95 TWLVNTYGEQIISYFDQYQNADDVCHAMNVCTS 127
+V+TYG I+S + + VC +++C+S
Sbjct: 359 QEVVDTYGRSILSILLDEASPELVCSMLHLCSS 391
>dbj|BAA95677.1| prosaposin [Bos taurus]
Length = 525
Score = 45.0 bits (105), Expect = 0.005
Identities = 27/93 (29%), Positives = 43/93 (46%), Gaps = 6/93 (6%)
Query: 39 EFISNDVKSANGGTHCAACTIVLSLVNQYSGIHNKSIED----SLDELCSFFPDEAGSLC 94
E I D A +C C V+ V + I N E+ +LD++CS P C
Sbjct: 301 EPIKKDPAPAKADIYCEVCEFVVKEVAKL--IDNNRTEEEILHALDKVCSKLPTSLAEQC 358
Query: 95 TWLVNTYGEQIISYFDQYQNADDVCHAMNVCTS 127
+V+TYG I+S + + VC +++C+S
Sbjct: 359 QEVVDTYGSSILSILLDEASPELVCSMLHLCSS 391
>emb|CAA40392.1| cerebroside sulfate activator [Homo sapiens]
Length = 210
Score = 41.9 bits (97), Expect = 0.047
Identities = 21/81 (25%), Positives = 39/81 (47%), Gaps = 2/81 (2%)
Query: 48 ANGGTHCAACTIVLSLVNQY--SGIHNKSIEDSLDELCSFFPDEAGSLCTWLVNTYGEQI 105
A +C C ++ V + + K I D+ D++CS P C +V+TYG I
Sbjct: 6 AKSDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSI 65
Query: 106 ISYFDQYQNADDVCHAMNVCT 126
+S + + + VC +++C+
Sbjct: 66 LSILLEEVSPELVCSMLHLCS 86
>pir||S21770 saposin-C - bovine
gb|AAB21926.1| saposin-C, co-glucosidase, sphingolipid activator protein A,
SAP-2=activator protein [cattle, spleen, Peptide, 80 aa]
Length = 80
Score = 41.5 bits (96), Expect = 0.053
Identities = 23/79 (29%), Positives = 39/79 (49%), Gaps = 6/79 (7%)
Query: 53 HCAACTIVLSLVNQYSGIHNKSIED----SLDELCSFFPDEAGSLCTWLVNTYGEQIISY 108
+C C V+ V + I N E+ +LD++CS P C +V+TYG I+S
Sbjct: 4 YCQVCEFVVKEVAKL--IDNNRTEEEILHALDKVCSKLPTSLAEQCQEVVDTYGRSILSI 61
Query: 109 FDQYQNADDVCHAMNVCTS 127
+ + VC +++C+S
Sbjct: 62 LLDEASPELVCSMLHLCSS 80
>gb|AAB06759.1| SapA
Length = 456
Score = 41.2 bits (95), Expect = 0.080
Identities = 25/90 (27%), Positives = 39/90 (42%), Gaps = 7/90 (7%)
Query: 52 THCAACTIVLSLVNQYSGIHNKSIEDSL---DELCSFFPDEAGSLCTWLVNTYGEQIISY 108
T C CT + + NK++ED + D+ C P + C + + Y II
Sbjct: 160 TDCEICTFISGYAENFLE-ENKTLEDIIKVVDDFCKILPAAYKTDCVAMASNYIPAIIKM 218
Query: 109 FDQYQNADDVCHAMNVCTSPTCRLFTSAPS 138
+ + VC +N+C +PT TS PS
Sbjct: 219 LENDNSPGQVCQKLNLCPAPTP---TSTPS 245
Score = 38.4 bits (88), Expect = 0.47
Identities = 18/76 (23%), Positives = 36/76 (46%), Gaps = 2/76 (2%)
Query: 54 CAACTIVLSLVNQY--SGIHNKSIEDSLDELCSFFPDEAGSLCTWLVNTYGEQIISYFDQ 111
C C V+ +Y S + +++++ L C+ P G +C +VN Y E ++ +
Sbjct: 256 CEICETVIGFAEKYLGSALDSQTVKTFLQNECNKLPGVVGDVCNEVVNEYLEFAVTQLEA 315
Query: 112 YQNADDVCHAMNVCTS 127
N ++C + CT+
Sbjct: 316 KFNPTEICQLFSFCTA 331
>pir||SAHUP saposin precursor [validated] - human
dbj|BAA00321.1| sphingolipid activator proteins [Homo sapiens]
prf||1504251A sphingolipid activator
Length = 527
Score = 40.8 bits (94), Expect = 0.098
Identities = 27/109 (24%), Positives = 48/109 (43%), Gaps = 2/109 (1%)
Query: 20 TAVNIPVNGKSLEIKNKFEEFISNDVKSANGGTHCAACTIVLSLVNQY--SGIHNKSIED 77
T V V K++ + E I A +C C ++ V + + K I D
Sbjct: 284 TLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILD 343
Query: 78 SLDELCSFFPDEAGSLCTWLVNTYGEQIISYFDQYQNADDVCHAMNVCT 126
+ D++CS P C +V+TYG I+S + + + VC +++C+
Sbjct: 344 AFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCS 392
>gb|AAA36594.1| cerebroside sulfate activator protein
Length = 527
Score = 40.8 bits (94), Expect = 0.098
Identities = 27/109 (24%), Positives = 48/109 (43%), Gaps = 2/109 (1%)
Query: 20 TAVNIPVNGKSLEIKNKFEEFISNDVKSANGGTHCAACTIVLSLVNQY--SGIHNKSIED 77
T V V K++ + E I A +C C ++ V + + K I D
Sbjct: 284 TLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILD 343
Query: 78 SLDELCSFFPDEAGSLCTWLVNTYGEQIISYFDQYQNADDVCHAMNVCT 126
+ D++CS P C +V+TYG I+S + + + VC +++C+
Sbjct: 344 AFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCS 392
>gb|AAA36596.1| cerebroside sulfate activator protein
Length = 526
Score = 40.8 bits (94), Expect = 0.098
Identities = 27/109 (24%), Positives = 48/109 (43%), Gaps = 2/109 (1%)
Query: 20 TAVNIPVNGKSLEIKNKFEEFISNDVKSANGGTHCAACTIVLSLVNQY--SGIHNKSIED 77
T V V K++ + E I A +C C ++ V + + K I D
Sbjct: 283 TLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILD 342
Query: 78 SLDELCSFFPDEAGSLCTWLVNTYGEQIISYFDQYQNADDVCHAMNVCT 126
+ D++CS P C +V+TYG I+S + + + VC +++C+
Sbjct: 343 AFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCS 391
>ref|NP_002769.1| prosaposin (variant Gaucher disease and variant metachromatic
leukodystrophy); Prosaposin (sphingolipid activator
protein-1) [Homo sapiens]
sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
gb|AAA52560.1| co-beta glucosidase precursor
gb|AAB59494.1| sphingolipid activator precursor
gb|AAA60303.1| prosaposin
gb|AAH04275.1|AAH04275 prosaposin (variant Gaucher disease and variant metachromatic
leukodystrophy) [Homo sapiens]
gb|AAH07612.1|AAH07612 prosaposin (variant Gaucher disease and variant metachromatic
leukodystrophy) [Homo sapiens]
gb|AAH01503.1|AAH01503 prosaposin (variant Gaucher disease and variant metachromatic
leukodystrophy) [Homo sapiens]
Length = 524
Score = 40.8 bits (94), Expect = 0.10
Identities = 27/109 (24%), Positives = 48/109 (43%), Gaps = 2/109 (1%)
Query: 20 TAVNIPVNGKSLEIKNKFEEFISNDVKSANGGTHCAACTIVLSLVNQY--SGIHNKSIED 77
T V V K++ + E I A +C C ++ V + + K I D
Sbjct: 281 TLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILD 340
Query: 78 SLDELCSFFPDEAGSLCTWLVNTYGEQIISYFDQYQNADDVCHAMNVCT 126
+ D++CS P C +V+TYG I+S + + + VC +++C+
Sbjct: 341 AFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCS 389
>gb|AAA36595.1| cerebroside sulfate activator protein
Length = 524
Score = 40.8 bits (94), Expect = 0.10
Identities = 27/109 (24%), Positives = 48/109 (43%), Gaps = 2/109 (1%)
Query: 20 TAVNIPVNGKSLEIKNKFEEFISNDVKSANGGTHCAACTIVLSLVNQY--SGIHNKSIED 77
T V V K++ + E I A +C C ++ V + + K I D
Sbjct: 281 TLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILD 340
Query: 78 SLDELCSFFPDEAGSLCTWLVNTYGEQIISYFDQYQNADDVCHAMNVCT 126
+ D++CS P C +V+TYG I+S + + + VC +++C+
Sbjct: 341 AFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCS 389
>ref|NP_524597.1| Saposin-related; Aggregation promoting activity; Drosophila
Aggregation promoting activity; 110kD protein
[Drosophila melanogaster]
gb|AAD38622.1|AF145647_1 BcDNA.GH08312 [Drosophila melanogaster]
gb|AAF57097.1| CG12070-PA [Drosophila melanogaster]
Length = 953
Score = 40.4 bits (93), Expect = 0.12
Identities = 20/78 (25%), Positives = 34/78 (42%), Gaps = 2/78 (2%)
Query: 50 GGTHCAACTIVLSLVNQYSGI--HNKSIEDSLDELCSFFPDEAGSLCTWLVNTYGEQIIS 107
GG C C +L + + + I+ +++ +C+ P C V YG+ +I+
Sbjct: 433 GGELCTLCEYMLHFIQETLATPSTDDEIKHTVENICAKLPSGVAGQCRNFVEMYGDAVIA 492
Query: 108 YFDQYQNADDVCHAMNVC 125
Q N DVC M +C
Sbjct: 493 LLVQGLNPRDVCPLMQMC 510
Score = 34.5 bits (78), Expect = 6.6
Identities = 28/108 (25%), Positives = 40/108 (36%), Gaps = 4/108 (3%)
Query: 29 KSLEIKNKFEEFISNDVKSANGGTHCAACTIVLSLVNQYSGIHNKS---IEDSLDELCSF 85
K+L K E F C C + Q NKS I+ L+ LCS
Sbjct: 512 KNLPKKEDVEVFNPQPASDEQDPPTCPLCLFAVEQA-QMKIRDNKSKDNIKKVLNGLCSH 570
Query: 86 FPDEAGSLCTWLVNTYGEQIISYFDQYQNADDVCHAMNVCTSPTCRLF 133
P+E C VNTY ++I ++C + +C T L+
Sbjct: 571 LPNEIKEECVDFVNTYSNELIDMLITDFKPQEICVQLKLCPKTTYALW 618
>gb|AAD45802.1|AF162129_1 ecdysone dependent glycoprotein [Drosophila melanogaster]
Length = 441
Score = 40.4 bits (93), Expect = 0.13
Identities = 20/78 (25%), Positives = 34/78 (42%), Gaps = 2/78 (2%)
Query: 50 GGTHCAACTIVLSLVNQYSGI--HNKSIEDSLDELCSFFPDEAGSLCTWLVNTYGEQIIS 107
GG C C +L + + + I+ +++ +C+ P C V YG+ +I+
Sbjct: 208 GGELCTLCEYMLHFIQETLATPSTDDEIKHTVENICAKLPSGVAGQCRNFVEMYGDAVIA 267
Query: 108 YFDQYQNADDVCHAMNVC 125
Q N DVC M +C
Sbjct: 268 LLVQGLNPRDVCPLMQMC 285
Score = 34.9 bits (79), Expect = 5.0
Identities = 32/128 (25%), Positives = 48/128 (37%), Gaps = 4/128 (3%)
Query: 29 KSLEIKNKFEEFISNDVKSANGGTHCAACTIVLSLVNQYSGIHNKS---IEDSLDELCSF 85
K+L K E F C C + Q NKS I+ L+ LCS
Sbjct: 287 KNLPKKEDVEVFNPQPASDEQDPPTCPLCLFAVEQA-QMKIRDNKSKDNIKKVLNGLCSH 345
Query: 86 FPDEAGSLCTWLVNTYGEQIISYFDQYQNADDVCHAMNVCTSPTCRLFTSAPSKFYNPDH 145
P+E C VNTY ++I ++C + +C T L+ S + D
Sbjct: 346 LPNEIKEECVDFVNTYSNELIDMLITDFKPQEICVQLKLCPKTTYALWDLRISLEDDVDG 405
Query: 146 VHQEYNKE 153
H+ ++E
Sbjct: 406 EHKSSSEE 413
>pir||T00207 P109 protein - silkworm
dbj|BAA23126.1| BmP109 [Bombyx mori]
Length = 965
Score = 38.0 bits (87), Expect = 0.71
Identities = 28/101 (27%), Positives = 46/101 (44%), Gaps = 10/101 (9%)
Query: 30 SLEIKNKFEEFISNDVKSANGGTHCAACTIVLSLVNQYSGI--HNKS---IEDSLDELCS 84
+L+I + EE DV S + C + L V Q + +N+S I +LD LC+
Sbjct: 619 ALQICPQTEEIRRVDVNSEK-----SNCPLCLFAVEQLESVLKNNRSEENIRKALDGLCT 673
Query: 85 FFPDEAGSLCTWLVNTYGEQIISYFDQYQNADDVCHAMNVC 125
+ S C V+TY Q++ NA ++C + +C
Sbjct: 674 RLSQKLQSECIDFVDTYSSQLVEMLVADMNAKEICVFLKLC 714
Score = 37.3 bits (85), Expect = 1.1
Identities = 19/81 (23%), Positives = 35/81 (42%), Gaps = 2/81 (2%)
Query: 54 CAACTIVLSLV-NQYSGIHN-KSIEDSLDELCSFFPDEAGSLCTWLVNTYGEQIISYFDQ 111
C C VL + +Q HN I+ ++ +C P + C V Y + +IS Q
Sbjct: 787 CVLCEFVLKEIDDQIKDKHNDDEIKKAVHGICKHMPKSVSAECDQFVEKYADLVISLLAQ 846
Query: 112 YQNADDVCHAMNVCTSPTCRL 132
+ +VC + +C + ++
Sbjct: 847 ELDPSEVCEELKLCKPESLKI 867
Score = 35.3 bits (80), Expect = 4.7
Identities = 20/74 (27%), Positives = 31/74 (41%), Gaps = 2/74 (2%)
Query: 54 CAACTIVLSLVN-QYSGIHNK-SIEDSLDELCSFFPDEAGSLCTWLVNTYGEQIISYFDQ 111
CA C L + Q S + ++ ++ E C PD C V YG +I+ Q
Sbjct: 550 CAFCQYFLHYLQVQLSDTRTEDKVKAAVQEACDALPDALNGECKEFVTQYGSAVIALLVQ 609
Query: 112 YQNADDVCHAMNVC 125
+ VC A+ +C
Sbjct: 610 EIDPASVCPALQIC 623
>ref|XP_192668.1| similar to Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
[Mus musculus]
Length = 557
Score = 37.6 bits (86), Expect = 0.76
Identities = 25/103 (24%), Positives = 48/103 (46%), Gaps = 12/103 (11%)
Query: 30 SLEIKNKFEEFISNDVKSANGGTHCAACTIVLSLVNQYSG-IHNKSIED----SLDELCS 84
+LE+ + +E+ ++ A+ C C V+ N++S I N + E+ L C+
Sbjct: 297 ALEMMDPYEQ----NLVQAHNVILCQTCQFVM---NKFSELIVNNATEELLVKGLSNACA 349
Query: 85 FFPDEAGSLCTWLVNTYGEQIISYFDQYQNADDVCHAMNVCTS 127
PD A + C +V T+G ++ F N +C + +C +
Sbjct: 350 LLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAA 392
>dbj|BAC40308.1| unnamed protein product [Mus musculus]
dbj|BAC41035.1| unnamed protein product [Mus musculus]
Length = 554
Score = 37.6 bits (86), Expect = 0.76
Identities = 25/103 (24%), Positives = 48/103 (46%), Gaps = 12/103 (11%)
Query: 30 SLEIKNKFEEFISNDVKSANGGTHCAACTIVLSLVNQYSG-IHNKSIED----SLDELCS 84
+LE+ + +E+ ++ A+ C C V+ N++S I N + E+ L C+
Sbjct: 294 ALEMMDPYEQ----NLVQAHNVILCQTCQFVM---NKFSELIVNNATEELLVKGLSNACA 346
Query: 85 FFPDEAGSLCTWLVNTYGEQIISYFDQYQNADDVCHAMNVCTS 127
PD A + C +V T+G ++ F N +C + +C +
Sbjct: 347 LLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAA 389
>ref|NP_035309.1| prosaposin [Mus musculus]
gb|AAB02695.1| prosaposin [Mus musculus]
Length = 557
Score = 37.6 bits (86), Expect = 0.82
Identities = 25/103 (24%), Positives = 48/103 (46%), Gaps = 12/103 (11%)
Query: 30 SLEIKNKFEEFISNDVKSANGGTHCAACTIVLSLVNQYSG-IHNKSIED----SLDELCS 84
+LE+ + +E+ ++ A+ C C V+ N++S I N + E+ L C+
Sbjct: 297 ALEMMDPYEQ----NLVQAHNVILCQTCQFVM---NKFSELIVNNATEELLVKGLSNACA 349
Query: 85 FFPDEAGSLCTWLVNTYGEQIISYFDQYQNADDVCHAMNVCTS 127
PD A + C +V T+G ++ F N +C + +C +
Sbjct: 350 LLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAA 392
CPU time: 143.53 user secs. 2.42 sys. secs 145.95 total secs.
Database: nr
Posted date: Jan 18, 2003 1:23 PM
Number of letters in database: 421,297,171
Number of sequences in database: 1,315,992
Lambda K H
0.319 0.136 0.428
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 517045990
Number of Sequences: 1315992
Number of extensions: 23276838
Number of successful extensions: 49954
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 25
Number of HSP's successfully gapped in prelim test: 27
Number of HSP's that attempted gapping in prelim test: 49846
Number of HSP's gapped (non-prelim): 107
length of query: 564
length of database: 421,297,171
effective HSP length: 55
effective length of query: 509
effective length of database: 348,917,611
effective search space: 177599063999
effective search space used: 177599063999
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 77 (34.4 bits)
