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GPI Anchor Biosynthesis Report: NP_491700.1 (GAA1 family, Caenorhabditis elegans)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (670 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 2

Distribution of 12 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans... 1051 0.0
pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans 790 0.0
gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melano... 696 0.0
ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mu... 639 0.0
ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila mel... 620 e-176
ref|NP_003792.1| (NM_003801) anchor attachment protein 1; anchor... 602 e-171
emb|CAB65611.1| (AL136078) putative glycosylphosphatidylinositol... 568 e-160
ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis t... 536 e-151
ref|NP_013189.1| (NC_001144) ER protein essential for attaching ... 496 e-139
pir||T46923 hypothetical protein DKFZp434E1512.1 - human >gi|701... 494 e-138
ref|NP_491701.1| (NM_059300) F33D11.9a.p [Caenorhabditis elegans... 175 3e-42
Sequences with E-value WORSE than threshold

ref|NP_244921.1| (NC_002570) BH4053~unknown conserved protein [B... 40 0.090
Alignments
>ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans]
 gb|AAK68360.1|AF039720_13 (AF039720) Hypothetical protein F33D11.9b [Caenorhabditis elegans]
          Length = 670

 Score = 1051 bits (2689), Expect = 0.0
 Identities = 670/670 (100%), Positives = 670/670 (100%)

Query: 1   MENLLKFTNKKLKLLEMALEISKDGGTTRPKILDKILKFRKVALIIAYIGAYLSAQWLFD 60
           MENLLKFTNKKLKLLEMALEISKDGGTTRPKILDKILKFRKVALIIAYIGAYLSAQWLFD
Sbjct: 1   MENLLKFTNKKLKLLEMALEISKDGGTTRPKILDKILKFRKVALIIAYIGAYLSAQWLFD 60

Query: 61  PVNMEVTRISEHSLMPGLVTPKFDKSGIAIQLYRRFSDLPKSKSQQELIHTIFSDLGLEC 120
           PVNMEVTRISEHSLMPGLVTPKFDKSGIAIQLYRRFSDLPKSKSQQELIHTIFSDLGLEC
Sbjct: 61  PVNMEVTRISEHSLMPGLVTPKFDKSGIAIQLYRRFSDLPKSKSQQELIHTIFSDLGLEC 120

Query: 121 FTHKWRSKVAGNPMNGENVYGFIRGPRNDGAEAQMIVVQLGRSEKSRRMMSRMLAFVDYA 180
           FTHKWRSKVAGNPMNGENVYGFIRGPRNDGAEAQMIVVQLGRSEKSRRMMSRMLAFVDYA
Sbjct: 121 FTHKWRSKVAGNPMNGENVYGFIRGPRNDGAEAQMIVVQLGRSEKSRRMMSRMLAFVDYA 180

Query: 181 KDQVYWARDIVIVFVDGGEKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQ 240
           KDQVYWARDIVIVFVDGGEKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQ
Sbjct: 181 KDQVYWARDIVIVFVDGGEKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQ 240

Query: 241 TGALIGGVVYDLSGMAVKGQHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHSRVAIFGT 300
           TGALIGGVVYDLSGMAVKGQHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHSRVAIFGT
Sbjct: 241 TGALIGGVVYDLSGMAVKGQHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHSRVAIFGT 300

Query: 301 LHRHSNPYMEYSPFIVPMRALYTQAFISIEGIHSVLGKYGVQGLTVGLSHDYSEKQTGQF 360
           LHRHSNPYMEYSPFIVPMRALYTQAFISIEGIHSVLGKYGVQGLTVGLSHDYSEKQTGQF
Sbjct: 301 LHRHSNPYMEYSPFIVPMRALYTQAFISIEGIHSVLGKYGVQGLTVGLSHDYSEKQTGQF 360

Query: 361 IEEVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQE 420
           IEEVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQE
Sbjct: 361 IEEVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQE 420

Query: 421 FQFPVVHLAFHVLGFFYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSWSLINS 480
           FQFPVVHLAFHVLGFFYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSWSLINS
Sbjct: 421 FQFPVVHLAFHVLGFFYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSWSLINS 480

Query: 481 FLIFFMIPLGPYVFLKEPVRCVPSMRIVCLLETSLVLYGICLTNFGLAILCTAFVVPIVF 540
           FLIFFMIPLGPYVFLKEPVRCVPSMRIVCLLETSLVLYGICLTNFGLAILCTAFVVPIVF
Sbjct: 481 FLIFFMIPLGPYVFLKEPVRCVPSMRIVCLLETSLVLYGICLTNFGLAILCTAFVVPIVF 540

Query: 541 LMTTRSIFRSSVLAILNPISVLAFVAINIPGMFGQLFPTISQDSWLAAAQSIALTAIRED 600
           LMTTRSIFRSSVLAILNPISVLAFVAINIPGMFGQLFPTISQDSWLAAAQSIALTAIRED
Sbjct: 541 LMTTRSIFRSSVLAILNPISVLAFVAINIPGMFGQLFPTISQDSWLAAAQSIALTAIRED 600

Query: 601 VEFKVNHFFLLCCFGYPIWNMLFACSMRYGPEPQGSLGIFGNGSDDDDGDDDDGPQKTNR 660
           VEFKVNHFFLLCCFGYPIWNMLFACSMRYGPEPQGSLGIFGNGSDDDDGDDDDGPQKTNR
Sbjct: 601 VEFKVNHFFLLCCFGYPIWNMLFACSMRYGPEPQGSLGIFGNGSDDDDGDDDDGPQKTNR 660

Query: 661 GHSTKKNKRE 670
           GHSTKKNKRE
Sbjct: 661 GHSTKKNKRE 670
>pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans
          Length = 885

 Score =  790 bits (2020), Expect = 0.0
 Identities = 500/518 (96%), Positives = 504/518 (96%), Gaps = 2/518 (0%)

Query: 1   MENLLKFTNKKLKLLEMALEISKDGGTTRPKILDKILKFRKVALIIAYIGAYLSAQWLFD 60
           MENLLKFTNKKLKLLEMALEISKDGGTTRPKILDKILKFRKVALIIAYIGAYLSAQWLFD
Sbjct: 263 MENLLKFTNKKLKLLEMALEISKDGGTTRPKILDKILKFRKVALIIAYIGAYLSAQWLFD 322

Query: 61  PVNMEVTRISEHSLMPGLVTPKFDKSGIAIQLYRRFSDLPKSKSQQELIHTIFSDLGLEC 120
           PVNMEVTRISEHSLMPGLVTPKFDKSGIAIQLYRRFSDLPKSKSQQELIHTIFSDLGLEC
Sbjct: 323 PVNMEVTRISEHSLMPGLVTPKFDKSGIAIQLYRRFSDLPKSKSQQELIHTIFSDLGLEC 382

Query: 121 FTHKWRSKVAGNPMNGENVYGFIRGPRNDGAEAQMIVVQLGRSEKSRRMMSRMLAFVDYA 180
           FTHKWRSKVAGNPMNGENVYGFIRGPRNDGAEAQMIVVQLGRSEKSRRMMSRMLAFVDYA
Sbjct: 383 FTHKWRSKVAGNPMNGENVYGFIRGPRNDGAEAQMIVVQLGRSEKSRRMMSRMLAFVDYA 442

Query: 181 KDQVYWARDIVIVFVDGGEKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQ 240
           KDQVYWARDIVIVFVDGGEKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQ
Sbjct: 443 KDQVYWARDIVIVFVDGGEKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQ 502

Query: 241 TGALIGGVVYDLSGMAVKGQHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHSRVAIFGT 300
           TGALIGGVVYDLSGMAVKGQHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHSRVAIFGT
Sbjct: 503 TGALIGGVVYDLSGMAVKGQHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHSRVAIFGT 562

Query: 301 LHRHSNPYMEYSPFIVPMRALYTQAFISIEGIHSVLGKYGVQGLTVGLSHDYSEKQTGQF 360
           LHRHSNPYMEYSPFIVPMRALYTQAFISIEGIHSVLGKYGVQGLTVGLSHDYSEKQTGQF
Sbjct: 563 LHRHSNPYMEYSPFIVPMRALYTQAFISIEGIHSVLGKYGVQGLTVGLSHDYSEKQTGQF 622

Query: 361 IEEVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQE 420
           IEEVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQE
Sbjct: 623 IEEVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQE 682

Query: 421 FQFPVVHLAFHVLGFFYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSWSLINS 480
           FQFPVVHLAFHVLGFFYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSWSLINS
Sbjct: 683 FQFPVVHLAFHVLGFFYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSWSLINS 742

Query: 481 FLIFFMIPLGPYVFLKEPVRCVPSMRIVCLLETSLVLY 518
           FLIFFMIPLGPYVFLK   R +    ++ +L    VL 
Sbjct: 743 FLIFFMIPLGPYVFLKT--RSIFRSSVLAILNPISVLA 778
>gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melanogaster]
          Length = 674

 Score =  696 bits (1779), Expect = 0.0
 Identities = 154/663 (23%), Positives = 272/663 (40%), Gaps = 89/663 (13%)

Query: 27  TTRPKILDKILK-FRKVALIIAYIGAYLSAQWLFDPVNMEVTRISEHSLMPGLVTP--KF 83
           +T+ K++D + +  RKV   + Y+        L  P     T +SE++L PGLV P  + 
Sbjct: 10  STQSKLVDGLARHVRKVCYAL-YVAGVAWFFCLALPEFNHGTYLSENALSPGLVYPEIRI 68

Query: 84  DKSGIAIQLYRRFSDLPK---SKSQQELIHTIFSDLGLECFTHKWRSKVA---GNPMNGE 137
           D + +AIQL        K   S +    I    ++ GLE  TH +  +     G   +G+
Sbjct: 69  DANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFGGGKEYHGK 128

Query: 138 NVYGFIRGPRNDGAEAQMIVVQLGRSEKSRRMMSR----MLAFVDYAKDQVYWARDIVIV 193
           N+YG +R PR    E  +       +      +S     +LAF D+A+ + YWA+D++ +
Sbjct: 129 NIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKNYWAKDLIFL 188

Query: 194 FVDGGEKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGALIGGVVYDLS 253
                      E+    + A+L  Y   +         +    + A+ G+L   +  ++ 
Sbjct: 189 IT---------EQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAALNIEVQ 239

Query: 254 GMAVKGQHIVNIQTNGLNGQQVNLDVFNGITKI-------ADSKHHSRVAIFGTLHRHSN 306
            + +     V+++  GLNG+  NLD+FN + +I       +  K   R        RHS 
Sbjct: 240 DLEIDH---VDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKK-----RRHSQ 291

Query: 307 PYMEYSPFIVPMRALYTQAFISIEGIHSVLGKYGVQGLTVGLS----HDYSEKQTG---- 358
            + E + F   +  L +Q+     G H +  +Y +  LT+  +    H   +   G    
Sbjct: 292 SHFEQN-FRQMLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAV 350

Query: 359 ---QFIEEVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEW 415
              + IE ++R LNN+LER HQS+F YV+  +  + SI  Y+  L  LV+   + AY  W
Sbjct: 351 PLLKAIEGIARSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTW 410

Query: 416 TKIQEFQFPVVHLAFHV-----------------LGFFYYIATTYVFQTIGTTFV--LQW 456
           + +      +   A  +                     Y     Y+  T+   F+  +  
Sbjct: 411 STLPATNAELEKAAGWLREHEQEAELEENLEPDKFELPYGSVLIYLTATLLIGFLCNVLP 470

Query: 457 MQWPFVGIDGCDGQFTPSWSLINSFLIFFMIPLGPYVFLKEPVRCVPSMRIVCLLETSLV 516
           +Q  F+ I       T S   + SFL      L P+V +  P   +  + +  LL     
Sbjct: 471 LQQYFLEIPMGAAPLTTS---VLSFLSLIGFVL-PFVVVLPP-GGLELLHVAFLLIYGCA 525

Query: 517 LYGICLTNFGLAILCTAFVVPIVFLMTT-----RSIFRSSV---LAILNPISVLAFVAIN 568
           L  I L NF L +      VP+V  + T     RS  R+++     ++NP+ V+  + + 
Sbjct: 526 LIVIGLLNFALGLFAAVLTVPLVIALETKEENSRSTLRNTIRLATLVMNPMMVVYVIVL- 584

Query: 569 IPGMFGQLFPTISQDSWL----AAAQSIALTAIREDVEFKVNHFFLLCCFGYPIWNMLFA 624
              M    FP +     +     AA   +   + + V +    +F++C    P+W + + 
Sbjct: 585 --AMTFYQFPELPVQKIMLRAATAAMDASAYGLIDSVIYGNWLYFVICTIFLPLWIICWT 642

Query: 625 CSM 627
            S+
Sbjct: 643 LSL 645
>ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mus musculus]
 dbj|BAA82589.1| (AB002136) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAA82591.1| (AB002138) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAB03274.1| (AB006970) GPI anchor attachment protein [Mus musculus]
 dbj|BAB03275.1| (AB006971) GPI anchor attachment protein [Mus musculus]
 gb|AAH06697.1|AAH06697 (BC006697) GPI anchor attachment protein 1 [Mus musculus]
          Length = 621

 Score =  639 bits (1631), Expect = 0.0
 Identities = 148/642 (23%), Positives = 256/642 (39%), Gaps = 93/642 (14%)

Query: 36  ILKFRKVALIIAYIGAYLSAQWLFDPVNMEVTRISEHSLMPGLVTPKFDKSGIAIQLYRR 95
           +L+      +++Y+        L  P   + T +SE+++   +V  +F     A    R 
Sbjct: 17  VLRLNTPLCVLSYVAGIAWFLALAFPPLTQRTYMSENAMGSTMVEEQFVGGDRARSFARD 76

Query: 96  FSDLPKSKSQQE--LIHTIFSDLGLECFTHKWRSKVAGNP-------MNGENVYGFIRGP 146
           F+   K         +      +GLE +T  +  K+           ++G NVYG +R P
Sbjct: 77  FAAHRKKPGALPVAWLERSMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTNVYGILRAP 136

Query: 147 RNDGAEAQMIVVQLGRSEKSRRMMSRMLAFVDYAKDQVYWARDIVIVFVDGGEKNDSIEK 206
           R+   E+ ++ V  G    + + +  +LA   + + Q+YWA+DI+ +  D          
Sbjct: 137 RSASTESLVLTVPCGPDATNSQAVGLLLALAAHFRGQIYWAKDIIFLVTD---------H 187

Query: 207 AAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGALIGGVVYDLSGMAVKGQHIVNIQ 266
                +A+L  Y  I     + S       +Q + GA+   V  +LS   V     +++ 
Sbjct: 188 DLLGTEAWLEAYHDINVTGIQSS------PLQGRAGAIQAAVALELSSDVVTS---LDVT 238

Query: 267 TNGLNGQQVNLDVFNGITKIADSKHHSRVAIFGTLHRHSNPYMEYSPFIVPMRALYT--- 323
             GLNGQ  NLD+ N            +  +  TL     P  +++    P++ L T   
Sbjct: 239 VEGLNGQLPNLDLLNLFQTFC-----QKGGLLCTLQGKLQP-QDWTSLEGPLQGLQTLLL 292

Query: 324 ----QAFISIEGIHSVLGKYGVQGLTV-GLS----HDYSEKQTGQFIEEVSRMLNNVLER 374
               QA     G H +  +YGV+ LT+ G++    + Y     G+ +E + R LN++LER
Sbjct: 293 MVLRQASGRPHGPHGLFLRYGVEALTLRGINSFRQYKYDLATVGKALEGMFRKLNHLLER 352

Query: 375 LHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQ----------EFQFP 424
           LHQS+F Y+L     F SI +Y+     L+  L + A   W ++           +   P
Sbjct: 353 LHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQLHQAGVNPEEAGKAPSP 412

Query: 425 VVH-LAFHVLGFFYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQF--TPSWSLINSF 481
               L    +G     A   + Q +G          P +G       F    + +++ + 
Sbjct: 413 GTPLLPTQGVGLASLTAPLLISQAMGLALYFL----PVLGQHLATQHFPVAEAEAVVLTL 468

Query: 482 L------IFFMIPLGPYVFLKEPVRCVPSMRIVCLLETSLVLYGICLTNFGLAILCTAFV 535
           L      +         V  + P R   ++++V L+  +L L  I L NF L  L  A +
Sbjct: 469 LAIYVAGLALPHNTHRVVNSQVPDRGWMALKLVALIYLALQLGCIALLNFSLGFLLAATM 528

Query: 536 VPIVFLMT---TRSIFRSSVLAILNPISVLAFVAINIPGMFGQLF---PTISQDSWLAAA 589
           VP   L      R+++ +++L + +P   L          FG LF     +     LA  
Sbjct: 529 VPAAALAKPHGPRTLY-AALLVVTSPAVTL----------FGSLFLWRELLEVPLSLAEG 577

Query: 590 QSIALTAIREDV----EFKVNHFFLLCCFGYP----IWNMLF 623
             + LTA+ + V     +    F +L    YP     WN+LF
Sbjct: 578 WQLFLTALAQGVLEHYTYGALLFPILALGLYPCWLLFWNVLF 619
>ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila melanogaster]
 gb|AAK77275.1| (AY047543) GH05723p [Drosophila melanogaster]
          Length = 561

 Score =  620 bits (1583), Expect = e-176
 Identities = 137/569 (24%), Positives = 234/569 (41%), Gaps = 74/569 (13%)

Query: 27  TTRPKILDKILK-FRKVALIIAYIGAYLSAQWLFDPVNMEVTRISEHSLMPGLVTP--KF 83
           +T+ K++D + +  RKV   + Y+        L  P     T +SE++L PGLV P  + 
Sbjct: 10  STQSKLVDGLARHVRKVCYAL-YVAGVAWFFCLALPEFNHGTYLSENALSPGLVYPEIRI 68

Query: 84  DKSGIAIQLYRRFSDLPK---SKSQQELIHTIFSDLGLECFTHKWRSKVA---GNPMNGE 137
           D + +AIQL        K   S +    I    ++ GLE  TH +  +     G   +G+
Sbjct: 69  DANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFGGGKEYHGK 128

Query: 138 NVYGFIRGPRNDGAEAQMIVVQLGRSEKSRRMMSR----MLAFVDYAKDQVYWARDIVIV 193
           N+YG +R PR    E  +       +      +S     +LAF D+A+ + YWA+D++ +
Sbjct: 129 NIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKNYWAKDLIFL 188

Query: 194 FVDGGEKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGALIGGVVYDLS 253
                      E+    + A+L  Y   +         +    + A+ G+L   +  ++ 
Sbjct: 189 IT---------EQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAALNIEVQ 239

Query: 254 GMAVKGQHIVNIQTNGLNGQQVNLDVFNGITKI-------ADSKHHSRVAIFGTLHRHSN 306
            + +     V+++  GLNG+  NLD+FN + +I       +  K   R        RHS 
Sbjct: 240 DLEIDH---VDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKK-----RRHSQ 291

Query: 307 PYMEYSPFIVPMRALYTQAFISIEGIHSVLGKYGVQGLTVGLS----HDYSEKQTG---- 358
            + E + F   +  L +Q+     G H +  +Y +  LT+  +    H   +   G    
Sbjct: 292 SHFEQN-FRQMLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAV 350

Query: 359 ---QFIEEVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEW 415
              + IE ++R LNN+LER HQS+F YV+  +  + SI  Y+  L  LV+   + AY  W
Sbjct: 351 PLLKAIEGIARSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTW 410

Query: 416 TKIQEFQFPVVHLAFHV-----------------LGFFYYIATTYVFQTIGTTFV--LQW 456
           + +      +   A  +                     Y     Y+  T+   F+  +  
Sbjct: 411 STLPATNAELEKAAGWLREHEQEAELEENLEPDKFELPYGSVLIYLTATLLIGFLCNVLP 470

Query: 457 MQWPFVGIDGCDGQFTPSWSLINSFLIFFMIPLGPYVFLKEPVRCVPSMRIVCLLETSLV 516
           +Q  F+ I       T S   + SFL      L P+V +  P   +  + +  LL     
Sbjct: 471 LQQYFLEIPMGAAPLTTS---VLSFLSLIGFVL-PFVVVLPP-GGLELLHVAFLLIYGCA 525

Query: 517 LYGICLTNFGLAILCTAFVVPIVFLMTTR 545
           L  I L NF L +      VP+V  + T+
Sbjct: 526 LIVIGLLNFALGLFAAVLTVPLVIALETK 554
>ref|NP_003792.1| (NM_003801) anchor attachment protein 1; anchor attachment protein
           1 (Gaa1p, yeast) homolog; glycophosphatidylinositol
           anchor attachment 1 [Homo sapiens]
 dbj|BAA24035.1| (AB006969) hGAA1 [Homo sapiens]
 dbj|BAA82588.1| (AB002135) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Homo sapiens]
 dbj|BAA82590.1| (AB002137) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Homo sapiens]
 gb|AAH03171.1|AAH03171 (BC003171) anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo
           sapiens]
 gb|AAH04129.1|AAH04129 (BC004129) anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo
           sapiens]
          Length = 621

 Score =  602 bits (1537), Expect = e-171
 Identities = 148/642 (23%), Positives = 257/642 (39%), Gaps = 93/642 (14%)

Query: 36  ILKFRKVALIIAYIGAYLSAQWLFDPVNMEVTRISEHSLMPGLVTPKFDKSGIAIQLYRR 95
           +L+      +++Y+        L  P   + T +SE+++   +V  +F     A    R 
Sbjct: 17  VLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGSTMVEEQFAGGDRARAFARD 76

Query: 96  FSDLPKSKSQQE--LIHTIFSDLGLECFTHKWRSKVAGNP-------MNGENVYGFIRGP 146
           F+   K         +      +GLE +T  +  K+           ++G NVYG +R P
Sbjct: 77  FAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTNVYGILRAP 136

Query: 147 RNDGAEAQMIVVQLGRSEKSRRMMSRMLAFVDYAKDQVYWARDIVIVFVDGGEKNDSIEK 206
           R    E+ ++ V  G    + + +  +LA   + + Q+YWA+DIV +           E 
Sbjct: 137 RAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVFLVT---------EH 187

Query: 207 AAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGALIGGVVYDLSGMAVKGQHIVNIQ 266
                +A+L  Y  +     + S       +Q + GA+   V  +LS   V     +++ 
Sbjct: 188 DLLGTEAWLEAYHDVNVTGMQSS------PLQGRAGAIQAAVALELSSDVVTS---LDVA 238

Query: 267 TNGLNGQQVNLDVFNGITKIADSKHHSRVAIFGTLHRHSNPYMEYSPFIVPMRALYT--- 323
             GLNGQ  NLD+ N            +  +  TL     P  +++    P++ L T   
Sbjct: 239 VEGLNGQLPNLDLLNLFQTFC-----QKGGLLCTLQGKLQP-EDWTSLDGPLQGLQTLLL 292

Query: 324 ----QAFISIEGIHSVLGKYGVQGLTV-GLS----HDYSEKQTGQFIEEVSRMLNNVLER 374
               QA     G H +  +Y V+ LT+ G++    + Y     G+ +E + R LN++LER
Sbjct: 293 MVLRQASGRPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLER 352

Query: 375 LHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQE----------FQFP 424
           LHQS+F+Y+L     F SI +Y+  +  L+  L + A   W ++ E             P
Sbjct: 353 LHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGP 412

Query: 425 VVHL-AFHVLGFFYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQF--TPSWSLINSF 481
            V L     +G    +A   + Q +G    +     P +G       F    + +++ + 
Sbjct: 413 SVPLPPSQGVGLASLVAPLLISQAMGLALYVL----PVLGQHVATQHFPVAEAEAVVLTL 468

Query: 482 L------IFFMIPLGPYVFLKEPVRCVPSMRIVCLLETSLVLYGICLTNFGLAILCTAFV 535
           L      +         V  + P R   ++++V L+  +L L  I LTNF L  L    +
Sbjct: 469 LAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTM 528

Query: 536 VPIVFLMT---TRSIFRSSVLAILNPISVLAFVAINIPGMFGQLF---PTISQDSWLAAA 589
           VP   L      R+++ +++L + +P + L           G LF           LA  
Sbjct: 529 VPTAALAKPHGPRTLY-AALLVLTSPAATLL----------GSLFLWRELQEAPLSLAEG 577

Query: 590 QSIALTAIREDV----EFKVNHFFLLCCFGYP----IWNMLF 623
             + L A+ + V     +    F LL    YP     WN+LF
Sbjct: 578 WQLFLAALAQGVLEHHTYGALLFPLLSLGLYPCWLLFWNVLF 619
>emb|CAB65611.1| (AL136078) putative glycosylphosphatidylinositol anchor attachment
           protein [Schizosaccharomyces pombe]
          Length = 581

 Score =  568 bits (1450), Expect = e-160
 Identities = 129/589 (21%), Positives = 240/589 (39%), Gaps = 77/589 (13%)

Query: 52  YLSAQWLFD-PVNMEVTR--ISEHSLMPGLVTPKFDK--------SGIAIQLYRRFSDLP 100
            +   W+F  P N  + R  +SE +L+PG V   F+         S  A   +       
Sbjct: 28  LIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFENRYSKTVSSSLTAANTWSHLDASV 87

Query: 101 KSKSQQELIHTIFSDLGLECFTHKWRSKVAGNPMNGENVYGFIRGPRNDGAEAQMIVVQL 160
            + +  + +  IF+ +GL      +   + G+  NG N    +R PR D  E+ ++ V  
Sbjct: 88  GTNTMYDDLEQIFTAMGLPTQKQNYSINIPGSEFNGSNFITTLRAPRGDATESLLLCVPW 147

Query: 161 ----GRSEKSRRMMSRMLAFVDYAKDQVYWARDIVIVFVDGGEKNDSIEKAAFALDAFLL 216
               G+  ++   ++  ++ + Y +    W++DI++V  D            +   +FL 
Sbjct: 148 KDHIGQYNEAG--VALAISLLKYFQGWSLWSKDIILVIFDD---------PVYGPSSFLT 196

Query: 217 KYQKIEALNSKKSITVEADEIQAQTGALIGGVVYDLSGMAVKGQHIVNIQTNGLNGQQVN 276
            Y        + +  +    ++ ++G++  G+  +L         ++ +     NGQ  N
Sbjct: 197 SY------FDQTTPYISYTPLKIRSGSIQAGLSLELVTTE-NNSDVLEVLYQATNGQLPN 249

Query: 277 LDVFNGITKIADSKHHSRVAIFGTLHRHSNPYMEYSPFIVPM-RALYTQAFISIEGIHSV 335
           LD+FN I++I   +H +          H+N    Y+  +  +   + TQA  ++   H++
Sbjct: 250 LDLFNTISRIF-MQHFNYPLRLQGYDFHANSGSSYTSRLKSLWMGMLTQAVSNVTSAHAL 308

Query: 336 LGKYGVQGLTVGLS----HDYSEKQTGQFIEEVSRMLNNVLERLHQSYFMYVLADDLHFS 391
             +Y +  LT+ +       +   + GQ IE   R LNN+LE LHQS+F Y + D LHF 
Sbjct: 309 FPQYRIDMLTLRMKVKDPFSFDMFRFGQAIESTFRSLNNLLEHLHQSFFFYFILDHLHFI 368

Query: 392 SIAMYIIPLTILVSPLVISAYFEWTKIQEFQFPVVHLAFHVLGFFYYIATTYV--FQTIG 449
           SI  Y+  + IL +  ++ AY  W   ++        +F +   F  IA  Y+    T  
Sbjct: 369 SIGNYMPSILILAASFMLGAYRHWINHEKKIDLWRPFSFWLFSIFCTIAAYYLVSSSTKI 428

Query: 450 TTFVLQWMQWPFVGIDGCDGQFTPSWSLINSFLIFFMIPLGPYVFLKEPVRCVPSMRIVC 509
           T F+  ++   F+GI       +    L+ S+ +     +   +F+              
Sbjct: 429 TVFIFLYLMLTFIGIIFSTFMTSEDAELVLSYDL-----MSKSLFI-------------- 469

Query: 510 LLETSLVLYGICLTNFGLAILCTAFVVPIVFLMTTRSIFRSSVLAILNPISVLAFVAINI 569
               S+V       NF L+ +    +VP+ F+    +   S + A+L   S   F+    
Sbjct: 470 ----SVVST----LNFSLSFVVAILLVPLQFISFRFNRRLSLLFAVLTYFSTFIFLCSLS 521

Query: 570 PGMFGQLFPTISQ-------DSWLAAAQSIALTAIREDVEFKVNHFFLL 611
             + G L P           +SWL    S+ +  +  ++ F V  FF L
Sbjct: 522 KILNGPLVPFWLWAKEYELFNSWLMP--SVFMILVLPEIIFSVTSFFSL 568
>ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis thaliana]
          Length = 696

 Score =  536 bits (1367), Expect = e-151
 Identities = 151/708 (21%), Positives = 255/708 (35%), Gaps = 133/708 (18%)

Query: 20  EISKDGGTTRPK---ILDKILKFRKVALIIAYI-GAYLSAQWLFDPVNMEVTRISEHSLM 75
           E+  D G+ + K   I+   +     + + + +  A      L  P+  + T ISE++LM
Sbjct: 11  EVKTDDGSPKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALM 70

Query: 76  PGLVTPKFDKSGIA-----IQLYRRFSDLPKSKSQQELIHTIFSDLGLECFTHKWRSKVA 130
           PG          ++     ++  + F  L      Q+LI    SD+G E    K+  +  
Sbjct: 71  PGSARSMLSNRDVSDGSKLVKDIKNF-RLNHEGQVQKLIGKYMSDMGAEVSYQKFHPE-- 127

Query: 131 GNPMN----------------------GENVYGFIRGPRNDGAEAQMIVVQL-----GRS 163
           GN  +                      G NV G IR PR DG E+ ++V        G  
Sbjct: 128 GNQFHPLHFFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDY 187

Query: 164 EK-SRRMMSRMLAFVDYAKDQVYWARDIVIVFVDGGEKNDSIEKAAFALDAFLLKYQKIE 222
           E  S  ++S + + +     +V W    +I  V      D    AA+  +     ++  +
Sbjct: 188 EDLSLGIVSSLFSLL----SRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSD 243

Query: 223 ALNSKKSITVEADEIQAQTGALIGGVVYDLSGMAVKGQHIVNIQTNGLNGQQVNLDVFNG 282
            L   +  T  AD  + + G +   +V  + G + K +  ++I     NGQ  NLD+ N 
Sbjct: 244 LLKCDEQNT--ADNFR-RAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINV 300

Query: 283 ITKIADSKH--------------HSRVAIF-------GTLHRHSNP----------YMEY 311
           +  +A  +                S + IF       G L    NP          Y+E 
Sbjct: 301 VNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEG 360

Query: 312 SPFIVPMRALYTQAFISIEGIHSVLGKYGVQGLTVGLS-----------HDYSEKQTGQF 360
           S  +    +LY+QA     G H     Y V  +T+ +S           HD+  +   + 
Sbjct: 361 SATLA--SSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLR-GARL 417

Query: 361 IEEVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQE 420
           +E   R +NN+LE+ HQS+F+Y+L     F S+ +Y+I   +LV+PL + A   +    +
Sbjct: 418 LEGTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCK 477

Query: 421 FQFPVVHLAFHVL-GFFYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPS----- 474
                 H        + +  A   VF      F++  + +    I    GQ +P+     
Sbjct: 478 SLTNSTHNPTENFKSWKWLDAAKQVFTLHLLGFIVTLLPY---FICQVPGQHSPTNRSIM 534

Query: 475 WSLINSFLIFFMIPLGPYVFLKEPVRCVPSMRIVCLLETSLVL---YGICL---TNFGLA 528
           W   +S L+       P      P           +L++  +     G+CL    NF  A
Sbjct: 535 WGTTSSSLLLITFVTMPGC---SPFSSRLHGTNWAILKSVTISAAFIGLCLMSIINFATA 591

Query: 529 ILCTAFVVPIVFLMTT-RSIFRSSVL-AILNPISVLAFVAINIPGMFGQL---------- 576
            +    +VP   +    +   RS  L ++L     +  + I  P MF  +          
Sbjct: 592 EIGALLLVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGLLGEGLV 651

Query: 577 -------FPTISQDSWLAAAQSIALTAIREDVEFKVNHFFLLCCFGYP 617
                  F T  +  W   A   A       V         LC   +P
Sbjct: 652 GLSLGGEFWTWLESLW---AWKSATYLYIGMVHLPCWLL-CLCILFHP 695
>ref|NP_013189.1| (NC_001144) ER protein essential for attaching GPI
           (glycosylphosphatidylinositol) to protein; Gaa1p
           [Saccharomyces cerevisiae]
 sp|P39012|GAA1_YEAST GAA1 PROTEIN
 pir||S45053 membrane protein END2 - yeast (Saccharomyces cerevisiae)
 emb|CAA55944.1| (X79409) Gaa1 [Saccharomyces cerevisiae]
 gb|AAB67592.1| (U53880) Gaa1p [Saccharomyces cerevisiae]
 emb|CAA97649.1| (Z73260) ORF YLR088w [Saccharomyces cerevisiae]
          Length = 614

 Score =  496 bits (1265), Expect = e-139
 Identities = 115/536 (21%), Positives = 215/536 (39%), Gaps = 79/536 (14%)

Query: 31  KILDK--ILKFRKVALIIAYIGAYLSAQWLFDPVNM---EVTRISEHSLMPGLVTPKFDK 85
           +I+D   + +   +  +I+ + A      +           T ISE++LMP      F +
Sbjct: 10  RIVDMGLVPRIIALLPVISMLCALFGFISIAILPMDGQYRRTYISENALMPSQAYSYFRE 69

Query: 86  SGIAI-QLYR-RFSDLPK--SKSQQELIHTIFSDLGLECFTHKWRSKVAGNPMNGENVYG 141
           S   I + YR +  ++    S  +  L+ +   + G        ++ +  N   GE +YG
Sbjct: 70  SEWNILRGYRSQIKEMVNMTSMERNNLMGSWLQEFGT-------KTAIYENEQYGETLYG 122

Query: 142 FIRGPRNDGAEAQMIVVQLGRSEKSRRMMSRML--AFVDYAKDQVYWARDIVIVFVDGGE 199
            +  PR DG EA ++ V    S+    +    L  +   +      W+++I++VF     
Sbjct: 123 VMHAPRGDGTEAMVLAVPWFNSDDEFNIGGAALGVSLARFFSRWPVWSKNIIVVFS---- 178

Query: 200 KNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGALIGGVVYDLSGMAVKG 259
                E    AL +++  Y                  +    G++   VV D S      
Sbjct: 179 -----ENPRAALRSWVEAYH---------------TSLDLTGGSIEAAVVLDYSSTEDFF 218

Query: 260 QHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHS-RVAIFGTLHRHSNPYMEYSPFIVPM 318
           ++ V I  +GLNG+  NLD+ N    I+ ++H   +V++ G           +S   +  
Sbjct: 219 EY-VEISYDGLNGELPNLDLVN--IAISITEHEGMKVSLHGLPSDQLTNNNFWSRLKILC 275

Query: 319 RALYTQAFISIEGIHS--VLGKYGVQGLTVGLSHDYSEKQT--GQFIEEVSRMLNNVLER 374
             +   A   ++  H       + +Q +T+    +     T  G+  E + R +NN+LE+
Sbjct: 276 LGIRDWALSGVKKPHGNEAFSGWRIQSVTLKAHGNSGHDITTFGRIPEAMFRSINNLLEK 335

Query: 375 LHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQEFQFPVVHLAFHVLG 434
            HQS+F Y+L     F SI+ Y      L S + +S  F  + +  F    ++ A+  + 
Sbjct: 336 FHQSFFFYLLLAPRQFVSISSY------LPSAVALSIAFAISSLNAF----INNAYANIS 385

Query: 435 FFY---YIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSWSLINSFLI---FFMIP 488
            F     +A    F ++  +FV+      F+ I        PS  L+ +  +   F  + 
Sbjct: 386 LFSEYNLVALLVWFVSLVISFVV---SQAFLLI--------PSSGLLMTISMASCFLPLI 434

Query: 489 LGPYVFLKEPVRCVPSMRIVCLLETSLVLYGICLTNFGLAILCTAFVVPIVFLMTT 544
           L   + + EP+     ++ V  L  SLV   + + NF +A+L      P+ F+ T 
Sbjct: 435 LSRKIHISEPLSYR--LKNVAFLYFSLVSTSLLMINFAMALLIGTLAFPMTFVKTI 488
>pir||T46923 hypothetical protein DKFZp434E1512.1 - human
 emb|CAB75660.1| (AL157437) hypothetical protein [Homo sapiens]
 gb|AAH06383.1|AAH06383 (BC006383) Unknown (protein for MGC:12663) [Homo sapiens]
          Length = 499

 Score =  494 bits (1259), Expect = e-138
 Identities = 130/535 (24%), Positives = 220/535 (40%), Gaps = 84/535 (15%)

Query: 134 MNGENVYGFIRGPRNDGAEAQMIVVQLGRSEKSRRMMSRMLAFVDYAKDQVYWARDIVIV 193
           ++G NVYG +R PR    E+ ++ V  G    + + +  +LA   + + Q+YWA+DIV +
Sbjct: 2   VSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVFL 61

Query: 194 FVDGGEKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGALIGGVVYDLS 253
                      E      +A+L  Y  +     + S       +Q + GA+   V  +LS
Sbjct: 62  VT---------EHDLLGTEAWLEAYHDVNVTGMQSS------PLQGRAGAIQAAVALELS 106

Query: 254 GMAVKGQHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHSRVAIFGTLHRHSNPYMEYSP 313
              V     +++   GLNGQ  NLD+ N            +  +  TL     P  +++ 
Sbjct: 107 SDVVTS---LDVAVEGLNGQLPNLDLLNLFQTFC-----QKGGLLCTLQGKLQP-EDWTS 157

Query: 314 FIVPMRALYT-------QAFISIEGIHSVLGKYGVQGLTV-GLS----HDYSEKQTGQFI 361
              P++ L T       QA     G H +  +Y V+ LT+ G++    + Y     G+ +
Sbjct: 158 LDGPLQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKAL 217

Query: 362 EEVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQE- 420
           E + R LN++LERLHQS+F+Y+L     F SI +Y+  +  L+  L + A   W ++ E 
Sbjct: 218 EGMFRKLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEA 277

Query: 421 ---------FQFPVVHL-AFHVLGFFYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQ 470
                       P V L     +G    +A   + Q +G    +     P +G       
Sbjct: 278 GMGLEEPGGAPGPSVPLPPSQGVGLASLVAPLLISQAMGLALYVL----PVLGQHVATQH 333

Query: 471 F--TPSWSLINSFL------IFFMIPLGPYVFLKEPVRCVPSMRIVCLLETSLVLYGICL 522
           F    + +++ + L      +         V  + P R   ++++V L+  +L L  I L
Sbjct: 334 FPVAEAEAVVLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCIAL 393

Query: 523 TNFGLAILCTAFVVPIVFLMT---TRSIFRSSVLAILNPISVLAFVAINIPGMFGQLF-- 577
           TNF L  L    +VP   L      R+++ +++L + +P + L           G LF  
Sbjct: 394 TNFSLGFLLATTMVPTAALAKPHGPRTLY-AALLVLTSPAATLL----------GSLFLW 442

Query: 578 -PTISQDSWLAAAQSIALTAIREDV----EFKVNHFFLLCCFGYP----IWNMLF 623
                    LA    + L A+ + V     +    F LL    YP     WN+LF
Sbjct: 443 RELQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLFPLLSLGLYPCWLLFWNVLF 497
>ref|NP_491701.1| (NM_059300) F33D11.9a.p [Caenorhabditis elegans]
 pir||T32772 hypothetical protein F33D11.9a - Caenorhabditis elegans
 gb|AAB96702.1| (AF039720) Hypothetical protein F33D11.9a [Caenorhabditis elegans]
          Length = 229

 Score =  175 bits (440), Expect = 3e-42
 Identities = 106/124 (85%), Positives = 110/124 (88%), Gaps = 2/124 (1%)

Query: 395 MYIIPLTILVSPLVISAYFEWTKIQEFQFPVVHLAFHVLGFFYYIATTYVFQTIGTTFVL 454
           MYIIPLTILVSPLVISAYFEWTKIQEFQFPVVHLAFHVLGFFYYIATTYVFQTIGTTFVL
Sbjct: 1   MYIIPLTILVSPLVISAYFEWTKIQEFQFPVVHLAFHVLGFFYYIATTYVFQTIGTTFVL 60

Query: 455 QWMQWPFVGIDGCDGQFTPSWSLINSFLIFFMIPLGPYVFLKEPVRCVPSMRIVCLLETS 514
           QWMQWPFVGIDGCDGQFTPSWSLINSFLIFFMIPLGPYVFLK   R +    ++ +L   
Sbjct: 61  QWMQWPFVGIDGCDGQFTPSWSLINSFLIFFMIPLGPYVFLKT--RSIFRSSVLAILNPI 118

Query: 515 LVLY 518
            VL 
Sbjct: 119 SVLA 122
>ref|NP_244921.1| (NC_002570) BH4053~unknown conserved protein [Bacillus halodurans]
 dbj|BAB07772.1| (AP001520) BH4053~unknown conserved protein [Bacillus halodurans]
          Length = 341

 Score = 40.4 bits (94), Expect = 0.090
 Identities = 42/191 (21%), Positives = 72/191 (36%), Gaps = 18/191 (9%)

Query: 439 IATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSWSLINSFLIFFMIPLGPYVFLKEP 498
           I    +F  +G  FV Q +    + ID       PS       L F  + + P V +   
Sbjct: 140 IIPILIFCLLGVLFVFQSLLGFPITIDFTQQGNWPSA------LTFTALNILPLVAVLGA 193

Query: 499 VRCVPSMRI---VCLLETSLVLYGICLT-NFGLAILCTAFVV---PIVFLMTTRSIFRSS 551
           V      +    V  + + L+L  I    N  L  +    VV   P+  +M     +   
Sbjct: 194 VGHRIRHQGEIWVASIGSGLILGAISFLYNESLIQVAGDLVVYEIPLFAIMKHYPYYMVL 253

Query: 552 VLAILNPISVLAFVAINIPGMFGQLFPTISQDSWLAAAQSIALTAIREDVEFKVNHFFLL 611
           V++ L  +++    A  I G+  ++   ++  SWL     +ALT +   V      F  L
Sbjct: 254 VMSGLLWLAIYTTAAAGIFGLSSRMGAVLNWPSWL-----LALTLLGFMVPLTTFGFSTL 308

Query: 612 CCFGYPIWNML 622
               YP++ +L
Sbjct: 309 IAVLYPLYGIL 319
CPU time:   148.79 user secs.	    4.31 sys. secs	  153.10 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.314    0.167    0.469 

Gapped
Lambda     K      H
   0.270   0.0586    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 376974645
Number of Sequences: 887402
Number of extensions: 17206726
Number of successful extensions: 78834
Number of sequences better than 10.0: 198
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 187
Number of HSP's that attempted gapping in prelim test: 78646
Number of HSP's gapped (non-prelim): 260
length of query: 670
length of database: 277,845,442
effective HSP length: 56
effective length of query: 614
effective length of database: 228,150,930
effective search space: 140084671020
effective search space used: 140084671020
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.7 bits)
S2: 77 (34.1 bits)