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GPI Anchor Biosynthesis Report: NP_197414.1 (GAA1 family, Arabidopsis thaliana)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (696 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 1

Distribution of 12 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis t... 1019 0.0
ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mu... 671 0.0
ref|NP_003792.1| (NM_003801) anchor attachment protein 1; anchor... 624 e-177
gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melano... 569 e-161
ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila mel... 557 e-157
pir||T46923 hypothetical protein DKFZp434E1512.1 - human >gi|701... 496 e-139
ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans... 418 e-115
pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans 407 e-112
emb|CAB65611.1| (AL136078) putative glycosylphosphatidylinositol... 407 e-112
ref|NP_013189.1| (NC_001144) ER protein essential for attaching ... 381 e-104
Sequences with E-value WORSE than threshold

gb|AAH08920.1|AAH08920 (BC008920) Unknown (protein for IMAGE:363... 36 1.6
ref|NP_391526.1| (NC_000964) similar to antibiotic resistance pr... 35 3.5
Alignments
>ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis thaliana]
          Length = 696

 Score = 1019 bits (2607), Expect = 0.0
 Identities = 696/696 (100%), Positives = 696/696 (100%)

Query: 1   MAAVNRDNEEEVKTDDGSPKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAK 60
           MAAVNRDNEEEVKTDDGSPKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAK
Sbjct: 1   MAAVNRDNEEEVKTDDGSPKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAK 60

Query: 61  NTYISENALMPGSARSMLSNRDVSDGSKLVKDIKNFRLNHEGQVQKLIGKYMSDMGAEVS 120
           NTYISENALMPGSARSMLSNRDVSDGSKLVKDIKNFRLNHEGQVQKLIGKYMSDMGAEVS
Sbjct: 61  NTYISENALMPGSARSMLSNRDVSDGSKLVKDIKNFRLNHEGQVQKLIGKYMSDMGAEVS 120

Query: 121 YQKFHPEGNQFHPLHFFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYD 180
           YQKFHPEGNQFHPLHFFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYD
Sbjct: 121 YQKFHPEGNQFHPLHFFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYD 180

Query: 181 FINGGDYEDLSLGIVSSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFK 240
           FINGGDYEDLSLGIVSSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFK
Sbjct: 181 FINGGDYEDLSLGIVSSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFK 240

Query: 241 VSDLLKCDEQNTADNFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINV 300
           VSDLLKCDEQNTADNFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINV
Sbjct: 241 VSDLLKCDEQNTADNFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINV 300

Query: 301 VNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEG 360
           VNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEG
Sbjct: 301 VNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEG 360

Query: 361 SATLASSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARLLEG 420
           SATLASSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARLLEG
Sbjct: 361 SATLASSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARLLEG 420

Query: 421 TIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKSLT 480
           TIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKSLT
Sbjct: 421 TIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKSLT 480

Query: 481 NSTHNPTENFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQHSPTNRSIMWGTTSS 540
           NSTHNPTENFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQHSPTNRSIMWGTTSS
Sbjct: 481 NSTHNPTENFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQHSPTNRSIMWGTTSS 540

Query: 541 SLLLITFVTMPGCSPFSSRLHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGALLLVP 600
           SLLLITFVTMPGCSPFSSRLHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGALLLVP
Sbjct: 541 SLLLITFVTMPGCSPFSSRLHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGALLLVP 600

Query: 601 TCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGLLGEGLVGLSLGGEFW 660
           TCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGLLGEGLVGLSLGGEFW
Sbjct: 601 TCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGLLGEGLVGLSLGGEFW 660

Query: 661 TWLESLWAWKSATYLYIGMVHLPCWLLCLCILFHPS 696
           TWLESLWAWKSATYLYIGMVHLPCWLLCLCILFHPS
Sbjct: 661 TWLESLWAWKSATYLYIGMVHLPCWLLCLCILFHPS 696
>ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mus musculus]
 dbj|BAA82589.1| (AB002136) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAA82591.1| (AB002138) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAB03274.1| (AB006970) GPI anchor attachment protein [Mus musculus]
 dbj|BAB03275.1| (AB006971) GPI anchor attachment protein [Mus musculus]
 gb|AAH06697.1|AAH06697 (BC006697) GPI anchor attachment protein 1 [Mus musculus]
          Length = 621

 Score =  671 bits (1713), Expect = 0.0
 Identities = 185/704 (26%), Positives = 283/704 (39%), Gaps = 117/704 (16%)

Query: 18  SPKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSM 77
           S  ++ R + R+ + L     V S V   A  LA L  P L + TY+SENA+        
Sbjct: 5   SDPVRRRALARIVLRLNTPLCVLSYVAGIAWFLA-LAFPPLTQRTYMSENAMGSTMVEEQ 63

Query: 78  LSNRDVSDGSKLVKDIKNFRLNHEGQVQKLIGKYMSDMGAEVSYQKFH---PEGNQFHPL 134
               D +      +D    R          + + M  +G EV  Q F    P  ++ H  
Sbjct: 64  FVGGDRA--RSFARDFAAHRKKPGALPVAWLERSMRSVGLEVYTQSFSRKLPFPDETHER 121

Query: 135 HFFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGI 194
           +  S                G NV GI+RAPR    ES+VL  P            ++G+
Sbjct: 122 YMVS----------------GTNVYGILRAPRSASTESLVLTVPCGP---DATNSQAVGL 162

Query: 195 VSSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCDEQNTAD 254
           + +L +      + +KDII+LV D    D     AWL  YH     V+ +     Q    
Sbjct: 163 LLALAAHFRGQIYWAKDIIFLVTDH---DLLGTEAWLEAYH--DINVTGIQSSPLQG--- 214

Query: 255 NFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVNYLAVHRQGFYVK 314
              RAG + AA+ L++   S     +L +  E  NGQ+PNLDL+N+              
Sbjct: 215 ---RAGAIQAAVALEL---SSDVVTSLDVTVEGLNGQLPNLDLLNL-------------- 254

Query: 315 VEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEGSATLASSLYSQALG 374
                        + F +    +  L   L P     +     L+G  TL   +  QA G
Sbjct: 255 ------------FQTFCQKGGLLCTLQGKLQPQDWTSLEGP--LQGLQTLLLMVLRQASG 300

Query: 375 IPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRG-ARLLEGTIRSVNNLLEKFH 433
            P GPHG F  Y V+A+TL+    F      RQ+ + L    + LEG  R +N+LLE+ H
Sbjct: 301 RPHGPHGLFLRYGVEALTLRGINSF------RQYKYDLATVGKALEGMFRKLNHLLERLH 354

Query: 434 QSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKSLTNSTHNPTENFKSW 493
           QSFF Y+L + S+F+S+G+YM A   L+  L + A  L++     L  +  NP E  K+ 
Sbjct: 355 QSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKALELWM----QLHQAGVNPEEAGKA- 409

Query: 494 KWLDAAKQVFTLHLLGFIVTLLPYFICQ----------VPGQHSPTNRSIMWGTTSSSLL 543
                   +     +G      P  I Q          V GQH  T    +    +  L 
Sbjct: 410 --PSPGTPLLPTQGVGLASLTAPLLISQAMGLALYFLPVLGQHLATQHFPVAEAEAVVLT 467

Query: 544 LITFVTMPGCSP------FSSRLHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGALL 597
           L+         P       +S++    W  LK V +    + L  ++++NF+   + A  
Sbjct: 468 LLAIYVAGLALPHNTHRVVNSQVPDRGWMALKLVALIYLALQLGCIALLNFSLGFLLAAT 527

Query: 598 LVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGLLGEGL-VGLSLG 656
           +VP   +A+P  P       ++L  A     LL +  P +    S  L  E L V LSL 
Sbjct: 528 MVPAAALAKPHGP-------RTLYAA-----LLVVTSPAVTLFGSLFLWRELLEVPLSLA 575

Query: 657 GEFWTWLESL-------WAWKSATYLYIGMVHLPCWLLCLCILF 693
             +  +L +L       + + +  +  + +   PCWLL   +LF
Sbjct: 576 EGWQLFLTALAQGVLEHYTYGALLFPILALGLYPCWLLFWNVLF 619
>ref|NP_003792.1| (NM_003801) anchor attachment protein 1; anchor attachment protein
           1 (Gaa1p, yeast) homolog; glycophosphatidylinositol
           anchor attachment 1 [Homo sapiens]
 dbj|BAA24035.1| (AB006969) hGAA1 [Homo sapiens]
 dbj|BAA82588.1| (AB002135) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Homo sapiens]
 dbj|BAA82590.1| (AB002137) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Homo sapiens]
 gb|AAH03171.1|AAH03171 (BC003171) anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo
           sapiens]
 gb|AAH04129.1|AAH04129 (BC004129) anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo
           sapiens]
          Length = 621

 Score =  624 bits (1592), Expect = e-177
 Identities = 181/702 (25%), Positives = 282/702 (39%), Gaps = 113/702 (16%)

Query: 18  SPKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSM 77
           S  ++ R + RL + L A   V S V   A  LA L+ P L + TY+SENA+        
Sbjct: 5   SDPVRRRALARLVLRLNAPLCVLSYVAGIAWFLA-LVFPPLTQRTYMSENAMGSTMVEEQ 63

Query: 78  LSNRDVSDGSKLVKDIKNFRLNHEGQVQKLIGKYMSDMGAEVSYQKFH---PEGNQFHPL 134
            +           +D    R          + + M  +G EV  Q F    P  ++ H  
Sbjct: 64  FAGG--DRARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHER 121

Query: 135 HFFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGI 194
           +  S                G NV GI+RAPR    ES+VL  P            ++G+
Sbjct: 122 YMVS----------------GTNVYGILRAPRAASTESLVLTVPCG---SDSTNSQAVGL 162

Query: 195 VSSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCDEQNTAD 254
           + +L +      + +KDI++LV +    D     AWL  YH     V+ +     Q    
Sbjct: 163 LLALAAHFRGQIYWAKDIVFLVTEH---DLLGTEAWLEAYH--DVNVTGMQSSPLQG--- 214

Query: 255 NFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVNYLAVHRQGFYVK 314
              RAG + AA+ L++   S     +L +  E  NGQ+PNLDL+N+              
Sbjct: 215 ---RAGAIQAAVALEL---SSDVVTSLDVAVEGLNGQLPNLDLLNL-------------- 254

Query: 315 VEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEGSATLASSLYSQALG 374
                        + F +    +  L   L P+    +     L+G  TL   +  QA G
Sbjct: 255 ------------FQTFCQKGGLLCTLQGKLQPEDWTSLDGP--LQGLQTLLLMVLRQASG 300

Query: 375 IPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRG-ARLLEGTIRSVNNLLEKFH 433
            P G HG F  Y+V+A+TL+    F      RQ+ + L    + LEG  R +N+LLE+ H
Sbjct: 301 RPHGSHGLFLRYRVEALTLRGINSF------RQYKYDLVAVGKALEGMFRKLNHLLERLH 354

Query: 434 QSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKS-----LTNSTHNPTE 488
           QSFFLY+L   S+F+S+G+YM A   L+  L + A  L++   ++            P+ 
Sbjct: 355 QSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSV 414

Query: 489 NFKSWKWLDAAKQVFTL---HLLGFIVTLLPYFICQVPGQHSPTNRSIMWGTTSSSLLLI 545
                + +  A  V  L     +G  + +LP     V GQH  T    +    +  L L+
Sbjct: 415 PLPPSQGVGLASLVAPLLISQAMGLALYVLP-----VLGQHVATQHFPVAEAEAVVLTLL 469

Query: 546 TFVTMPGCSP------FSSRLHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGALLLV 599
                    P       S++     W  LK V +    + L  +++ NF+   + A  +V
Sbjct: 470 AIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMV 529

Query: 600 PTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGLLGEGL-VGLSLGGE 658
           PT  +A+P  P       ++L  A     LL +  P      S  L  E     LSL   
Sbjct: 530 PTAALAKPHGP-------RTLYAA-----LLVLTSPAATLLGSLFLWRELQEAPLSLAEG 577

Query: 659 FWTWLESL-------WAWKSATYLYIGMVHLPCWLLCLCILF 693
           +  +L +L         + +  +  + +   PCWLL   +LF
Sbjct: 578 WQLFLAALAQGVLEHHTYGALLFPLLSLGLYPCWLLFWNVLF 619
>gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melanogaster]
          Length = 674

 Score =  569 bits (1452), Expect = e-161
 Identities = 160/683 (23%), Positives = 271/683 (39%), Gaps = 95/683 (13%)

Query: 38  PVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSMLSNRDVSDGSKLVKDIKNFR 97
                V   A     L LP     TY+SENAL PG     +         +L+++++  R
Sbjct: 27  CYALYVAGVAWFFC-LALPEFNHGTYLSENALSPGLVYPEIRIDANRLAIQLLEELQRER 85

Query: 98  LNHEG-QVQKLIGKYMSDMGAEVSYQKFHPEGNQFHPLHFFSGPDSYTLLENVSCASYGV 156
            +H        I   M++ G E     +          + F G   Y          +G 
Sbjct: 86  KDHLSTTPHAWIAAKMNEFGLETHTHNYTL-------RYPFGGGKEY----------HGK 128

Query: 157 NVAGIIRAPRGDGKESIVLVTPYDFINGGDYE-DLSLGIVSSLFSLLSRVTWLSKDIIWL 215
           N+ GI+RAPR    E IV   PY   +    +   S+ ++ +      R  + +KD+I+L
Sbjct: 129 NIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKNYWAKDLIFL 188

Query: 216 VADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCDEQNTADNFRRAGTMAAALVLKVDGRSE 275
           + +        + AWL  YH    ++ DL K       +   RAG++ AAL ++V    +
Sbjct: 189 ITEQE---QLGMQAWLEAYHDGDREL-DLSKA-YLRPGNLPARAGSLQAALNIEV----Q 239

Query: 276 KFE-DTLSIYAEASNGQMPNLDLINVVNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIF 334
             E D + +  E  NG++PNLD+ N+V  +                    +   I     
Sbjct: 240 DLEIDHVDVRIEGLNGKLPNLDMFNLVQRIM-------------------AREGIASGYK 280

Query: 335 EAVGKLAHMLNPDWNFGIPAADYLEGSATLASSLYSQALGIPTGPHGAFRDYQVDAITLK 394
           +A  K               + + +    + + L SQ+ G+PTG HG F  Y++DA+T+ 
Sbjct: 281 QAPRKKRRHS---------QSHFEQNFRQMLTMLASQSSGVPTGNHGLFHRYRIDALTIA 331

Query: 395 VSPRFPPDS-KTRQHDFFLRGARLLEGTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVY 453
            + R    + K       +   + +EG  RS+NNLLE+FHQSFF Y++ S  ++IS+G Y
Sbjct: 332 ANRRATHATLKGSPGSAAVPLLKAIEGIARSLNNLLERFHQSFFFYVIVSNDRYISIGDY 391

Query: 454 MIAFALLVA-----------PLPMVAASL-----YIDGCKSLTNSTHNPTENFKSWKWLD 497
           M A   LVA            LP   A L     ++   +       N   +     +  
Sbjct: 392 MPALVALVACAFLKAYLTWSTLPATNAELEKAAGWLREHEQEAELEENLEPDKFELPYGS 451

Query: 498 AAKQVFTLHLLGFIVTLLPY--FICQVPGQHSPTNRSIMWGTTSSSLLLITFVTMPGCSP 555
               +    L+GF+  +LP   +  ++P   +P   S++     S L LI FV      P
Sbjct: 452 VLIYLTATLLIGFLCNVLPLQQYFLEIPMGAAPLTTSVL-----SFLSLIGFV-----LP 501

Query: 556 FSSRLHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGALLLVPTCLMAQPIKPALRSR 615
           F   L      +L    +      L ++ ++NFA     A+L VP  +  +  +   RS 
Sbjct: 502 FVVVLPPGGLELLHVAFLLIYGCALIVIGLLNFALGLFAAVLTVPLVIALETKEENSRST 561

Query: 616 RLKSLLGAFCSIVLLTIGFPVMFFAISKGLLGEGLV-GLSLGGEFWTWLESLWA------ 668
              ++  A   +  + + + V+  A++     E  V  + L         S +       
Sbjct: 562 LRNTIRLATLVMNPMMVVY-VIVLAMTFYQFPELPVQKIMLRAATAAMDASAYGLIDSVI 620

Query: 669 WKSATYLYIGMVHLPCWLLCLCI 691
           + +  Y  I  + LP W++C  +
Sbjct: 621 YGNWLYFVICTIFLPLWIICWTL 643
>ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila melanogaster]
 gb|AAK77275.1| (AY047543) GH05723p [Drosophila melanogaster]
          Length = 561

 Score =  557 bits (1421), Expect = e-157
 Identities = 146/600 (24%), Positives = 241/600 (39%), Gaps = 87/600 (14%)

Query: 38  PVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSMLSNRDVSDGSKLVKDIKNFR 97
                V   A     L LP     TY+SENAL PG     +         +L+++++  R
Sbjct: 27  CYALYVAGVAWFFC-LALPEFNHGTYLSENALSPGLVYPEIRIDANRLAIQLLEELQRER 85

Query: 98  LNHEG-QVQKLIGKYMSDMGAEVSYQKFHPEGNQFHPLHFFSGPDSYTLLENVSCASYGV 156
            +H        I   M++ G E     +          + F G   Y          +G 
Sbjct: 86  KDHLSTTPHAWIAAKMNEFGLETHTHNYTL-------RYPFGGGKEY----------HGK 128

Query: 157 NVAGIIRAPRGDGKESIVLVTPYDFINGGDYE-DLSLGIVSSLFSLLSRVTWLSKDIIWL 215
           N+ GI+RAPR    E IV   PY   +    +   S+ ++ +      R  + +KD+I+L
Sbjct: 129 NIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKNYWAKDLIFL 188

Query: 216 VADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCDEQNTADNFRRAGTMAAALVLKVDGRSE 275
           + +        + AWL  YH    ++ DL K       +   RAG++ AAL ++V    +
Sbjct: 189 ITEQE---QLGMQAWLEAYHDGDREL-DLSKA-YLRPGNLPARAGSLQAALNIEV----Q 239

Query: 276 KFE-DTLSIYAEASNGQMPNLDLINVVNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIF 334
             E D + +  E  NG++PNLD+ N+V  +                    +   I     
Sbjct: 240 DLEIDHVDVRIEGLNGKLPNLDMFNLVQRIM-------------------AREGIASGYK 280

Query: 335 EAVGKLAHMLNPDWNFGIPAADYLEGSATLASSLYSQALGIPTGPHGAFRDYQVDAITLK 394
           +A  K               + + +    + + L SQ+ G+PTG HG F  Y++DA+T+ 
Sbjct: 281 QAPRKKRRHS---------QSHFEQNFRQMLTMLASQSSGVPTGNHGLFHRYRIDALTIA 331

Query: 395 VSPRFPPDS-KTRQHDFFLRGARLLEGTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVY 453
            + R    + K       +   + +EG  RS+NNLLE+FHQSFF Y++ S  ++IS+G Y
Sbjct: 332 ANRRATHATLKGSPGSAAVPLLKAIEGIARSLNNLLERFHQSFFFYVIVSNDRYISIGDY 391

Query: 454 MIAFALLVA-----------PLPMVAASL-----YIDGCKSLTNSTHNPTENFKSWKWLD 497
           M A   LVA            LP   A L     ++   +       N   +     +  
Sbjct: 392 MPALVALVACAFLKAYLTWSTLPATNAELEKAAGWLREHEQEAELEENLEPDKFELPYGS 451

Query: 498 AAKQVFTLHLLGFIVTLLPY--FICQVPGQHSPTNRSIMWGTTSSSLLLITFVTMPGCSP 555
               +    L+GF+  +LP   +  ++P   +P   S++     S L LI FV      P
Sbjct: 452 VLIYLTATLLIGFLCNVLPLQQYFLEIPMGAAPLTTSVL-----SFLSLIGFV-----LP 501

Query: 556 FSSRLHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGALLLVPTCLMAQPIKPALRSR 615
           F   L      +L    +      L ++ ++NFA     A+L VP  +  +  +    +R
Sbjct: 502 FVVVLPPGGLELLHVAFLLIYGCALIVIGLLNFALGLFAAVLTVPLVIALETKEENSAAR 561
>pir||T46923 hypothetical protein DKFZp434E1512.1 - human
 emb|CAB75660.1| (AL157437) hypothetical protein [Homo sapiens]
 gb|AAH06383.1|AAH06383 (BC006383) Unknown (protein for MGC:12663) [Homo sapiens]
          Length = 499

 Score =  496 bits (1265), Expect = e-139
 Identities = 149/562 (26%), Positives = 234/562 (41%), Gaps = 91/562 (16%)

Query: 155 GVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGIVSSLFSLLSRVTWLSKDIIW 214
           G NV GI+RAPR    ES+VL  P            ++G++ +L +      + +KDI++
Sbjct: 4   GTNVYGILRAPRAASTESLVLTVPCG---SDSTNSQAVGLLLALAAHFRGQIYWAKDIVF 60

Query: 215 LVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCDEQNTADNFRRAGTMAAALVLKVDGRS 274
           LV +    D     AWL  YH     V+ +     Q       RAG + AA+ L++   S
Sbjct: 61  LVTEH---DLLGTEAWLEAYH--DVNVTGMQSSPLQG------RAGAIQAAVALEL---S 106

Query: 275 EKFEDTLSIYAEASNGQMPNLDLINVVNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIF 334
                +L +  E  NGQ+PNLDL+N+                           + F +  
Sbjct: 107 SDVVTSLDVAVEGLNGQLPNLDLLNL--------------------------FQTFCQKG 140

Query: 335 EAVGKLAHMLNPDWNFGIPAADYLEGSATLASSLYSQALGIPTGPHGAFRDYQVDAITLK 394
             +  L   L P+    +     L+G  TL   +  QA G P G HG F  Y+V+A+TL+
Sbjct: 141 GLLCTLQGKLQPEDWTSLDGP--LQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLR 198

Query: 395 VSPRFPPDSKTRQHDFFLRG-ARLLEGTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVY 453
               F      RQ+ + L    + LEG  R +N+LLE+ HQSFFLY+L   S+F+S+G+Y
Sbjct: 199 GINSF------RQYKYDLVAVGKALEGMFRKLNHLLERLHQSFFLYLLPGLSRFVSIGLY 252

Query: 454 MIAFALLVAPLPMVAASLYIDGCKS-----LTNSTHNPTENFKSWKWLDAAKQVFTL--- 505
           M A   L+  L + A  L++   ++            P+      + +  A  V  L   
Sbjct: 253 MPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPPSQGVGLASLVAPLLIS 312

Query: 506 HLLGFIVTLLPYFICQVPGQHSPTNRSIMWGTTSSSLLLITFVTMPGCSP------FSSR 559
             +G  + +LP     V GQH  T    +    +  L L+         P       S++
Sbjct: 313 QAMGLALYVLP-----VLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPHNTHRVVSTQ 367

Query: 560 LHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGALLLVPTCLMAQPIKPALRSRRLKS 619
                W  LK V +    + L  +++ NF+   + A  +VPT  +A+P  P       ++
Sbjct: 368 APDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKPHGP-------RT 420

Query: 620 LLGAFCSIVLLTIGFPVMFFAISKGLLGEGL-VGLSLGGEFWTWLESL-------WAWKS 671
           L  A     LL +  P      S  L  E     LSL   +  +L +L         + +
Sbjct: 421 LYAA-----LLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGA 475

Query: 672 ATYLYIGMVHLPCWLLCLCILF 693
             +  + +   PCWLL   +LF
Sbjct: 476 LLFPLLSLGLYPCWLLFWNVLF 497
>ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans]
 gb|AAK68360.1|AF039720_13 (AF039720) Hypothetical protein F33D11.9b [Caenorhabditis elegans]
          Length = 670

 Score =  418 bits (1063), Expect = e-115
 Identities = 137/657 (20%), Positives = 239/657 (35%), Gaps = 132/657 (20%)

Query: 11  EVKTDDGSPKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALM 70
           E+  D G+       I+   +     + + + +  A      L  P+  + T ISE++LM
Sbjct: 20  EISKDGGT---TRPKILDKILKFRKVALIIAYI-GAYLSAQWLFDPVNMEVTRISEHSLM 75

Query: 71  PGSARSMLSNRDVSDGSKLVKDIKNFR-LNHEGQVQKLIGKYMSDMGAEVSYQKFHPEGN 129
           PG          ++     ++  + F  L      Q+LI    SD+G E    K+  +  
Sbjct: 76  PGLVTPKFDKSGIA-----IQLYRRFSDLPKSKSQQELIHTIFSDLGLECFTHKWRSKV- 129

Query: 130 QFHPLHFFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYED 189
             +P++                   G NV G IR PR DG E+ ++V        G  E 
Sbjct: 130 AGNPMN-------------------GENVYGFIRGPRNDGAEAQMIVV-----QLGRSEK 165

Query: 190 LSLGIVSSLFSLL----SRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLL 245
            S  ++S + + +     +V W    +I  V      D    AA+  +     ++  + L
Sbjct: 166 -SRRMMSRMLAFVDYAKDQVYWARDIVIVFVDGGEKNDSIEKAAFALDAFLLKYQKIEAL 224

Query: 246 KCDEQNT--ADNFR-RAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVN 302
              +  T  AD  + + G +   +V  + G + K +  ++I     NGQ  NLD+ N + 
Sbjct: 225 NSKKSITVEADEIQAQTGALIGGVVYDLSGMAVKGQHIVNIQTNGLNGQQVNLDVFNGIT 284

Query: 303 YLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEGSA 362
            +A  +                S + IF       G L    NP          Y+E S 
Sbjct: 285 KIADSKH--------------HSRVAIF-------GTLHRHSNP----------YMEYSP 313

Query: 363 TLA--SSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLR-GARLLE 419
            +    +LY+QA     G H     Y V  +T+ +S           HD+  +   + +E
Sbjct: 314 FIVPMRALYTQAFISIEGIHSVLGKYGVQGLTVGLS-----------HDYSEKQTGQFIE 362

Query: 420 GTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKSL 479
              R +NN+LE+ HQS+F+Y+L     F S+ +Y+I   +LV+PL + A   +       
Sbjct: 363 EVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEW------- 415

Query: 480 TNSTHNPTENFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQHSPT---------- 529
                      + +++          H+LGF   +   ++ Q  G               
Sbjct: 416 --------TKIQEFQFPVVH---LAFHVLGFFYYIATTYVFQTIGTTFVLQWMQWPFVGI 464

Query: 530 -----NRSIMWGTTSSSLLLITFVTMPGCSPFSSRLHGTNWAILKSVTISAAFIGLCLMS 584
                  +  W   +S L+   F  +P       +        ++ V +    + L  + 
Sbjct: 465 DGCDGQFTPSWSLINSFLI---FFMIPLGPYVFLKEPVRCVPSMRIVCLLETSLVLYGIC 521

Query: 585 IINFATAEIGALLLVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLL---TIGFPVMF 638
           + NF  A +    +VP   +        RS    S+L     I +L    I  P MF
Sbjct: 522 LTNFGLAILCTAFVVPIVFLMTT-----RSIFRSSVLAILNPISVLAFVAINIPGMF 573
>pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans
          Length = 885

 Score =  407 bits (1036), Expect = e-112
 Identities = 115/525 (21%), Positives = 201/525 (37%), Gaps = 106/525 (20%)

Query: 11  EVKTDDGSPKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALM 70
           E+  D G+       I+   +     + + + +  A      L  P+  + T ISE++LM
Sbjct: 282 EISKDGGT---TRPKILDKILKFRKVALIIAYI-GAYLSAQWLFDPVNMEVTRISEHSLM 337

Query: 71  PGSARSMLSNRDVSDGSKLVKDIKNFR-LNHEGQVQKLIGKYMSDMGAEVSYQKFHPEGN 129
           PG          ++     ++  + F  L      Q+LI    SD+G E    K+  +  
Sbjct: 338 PGLVTPKFDKSGIA-----IQLYRRFSDLPKSKSQQELIHTIFSDLGLECFTHKWRSKV- 391

Query: 130 QFHPLHFFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYED 189
             +P++                   G NV G IR PR DG E+ ++V        G  E 
Sbjct: 392 AGNPMN-------------------GENVYGFIRGPRNDGAEAQMIVV-----QLGRSEK 427

Query: 190 LSLGIVSSLFSLL----SRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLL 245
            S  ++S + + +     +V W    +I  V      D    AA+  +     ++  + L
Sbjct: 428 -SRRMMSRMLAFVDYAKDQVYWARDIVIVFVDGGEKNDSIEKAAFALDAFLLKYQKIEAL 486

Query: 246 KCDEQNT--ADNFR-RAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVN 302
              +  T  AD  + + G +   +V  + G + K +  ++I     NGQ  NLD+ N + 
Sbjct: 487 NSKKSITVEADEIQAQTGALIGGVVYDLSGMAVKGQHIVNIQTNGLNGQQVNLDVFNGIT 546

Query: 303 YLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEGSA 362
            +A  +                S + IF       G L    NP          Y+E S 
Sbjct: 547 KIADSKH--------------HSRVAIF-------GTLHRHSNP----------YMEYSP 575

Query: 363 TLA--SSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLR-GARLLE 419
            +    +LY+QA     G H     Y V  +T+ +S           HD+  +   + +E
Sbjct: 576 FIVPMRALYTQAFISIEGIHSVLGKYGVQGLTVGLS-----------HDYSEKQTGQFIE 624

Query: 420 GTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKSL 479
              R +NN+LE+ HQS+F+Y+L     F S+ +Y+I   +LV+PL + A   +       
Sbjct: 625 EVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEW------- 677

Query: 480 TNSTHNPTENFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPG 524
                      + +++          H+LGF   +   ++ Q  G
Sbjct: 678 --------TKIQEFQFPVVH---LAFHVLGFFYYIATTYVFQTIG 711
>emb|CAB65611.1| (AL136078) putative glycosylphosphatidylinositol anchor attachment
           protein [Schizosaccharomyces pombe]
          Length = 581

 Score =  407 bits (1035), Expect = e-112
 Identities = 148/675 (21%), Positives = 259/675 (37%), Gaps = 150/675 (22%)

Query: 27  VRLGIFLIAHSPVFSVVFSAAGVLALLLLP--LLAKNTYISENALMPGSARSMLSNRDVS 84
           +R+  FL  H     +  +  G+  + +LP   +    ++SE+AL+PG   +   NR   
Sbjct: 9   IRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFENRYSK 68

Query: 85  DGSKLVKDIKNFR-LNHEGQVQKL---IGKYMSDMGAEVSYQKFHPEGNQFHPLHFFSGP 140
             S  +     +  L+       +   + +  + MG     Q +                
Sbjct: 69  TVSSSLTAANTWSHLDASVGTNTMYDDLEQIFTAMGLPTQKQNYS--------------- 113

Query: 141 DSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPY-DFINGGDYEDLSLGIVSSLF 199
                +        G N    +RAPRGD  ES++L  P+ D I  G Y +  + +  SL 
Sbjct: 114 -----INIPGSEFNGSNFITTLRAPRGDATESLLLCVPWKDHI--GQYNEAGVALAISLL 166

Query: 200 SLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYH---SPSFKVSDLLKCDEQNTADNF 256
                 +  SKDII ++ D         +++LT Y    +P    + L            
Sbjct: 167 KYFQGWSLWSKDIILVIFDDPVY---GPSSFLTSYFDQTTPYISYTPLKI---------- 213

Query: 257 RRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVNYLAVHRQGFYVKVE 316
            R+G++ A L L++   +E   D L +  +A+NGQ+PNLDL N ++ + +    + ++++
Sbjct: 214 -RSGSIQAGLSLEL-VTTENNSDVLEVLYQATNGQLPNLDLFNTISRIFMQHFNYPLRLQ 271

Query: 317 ----------KVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEGSATLAS 366
                        S L S W+ +  +    V   AH L P                    
Sbjct: 272 GYDFHANSGSSYTSRLKSLWMGMLTQAVSNVTS-AHALFPQ------------------- 311

Query: 367 SLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARLLEGTIRSVN 426
                              Y++D +TL++  + P        D F R  + +E T RS+N
Sbjct: 312 -------------------YRIDMLTLRMKVKDP-----FSFDMF-RFGQAIESTFRSLN 346

Query: 427 NLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKSLTNSTHNP 486
           NLLE  HQSFF Y +     FIS+G YM +  +L A   + A   +I           N 
Sbjct: 347 NLLEHLHQSFFFYFILDHLHFISIGNYMPSILILAASFMLGAYRHWI-----------NH 395

Query: 487 TENFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQHSPTNRSIMWGTTSSSLLLIT 546
            +    W+        F+  L     T+  Y++       S T  ++         L++T
Sbjct: 396 EKKIDLWR-------PFSFWLFSIFCTIAAYYLVS-----SSTKITVFIFL----YLMLT 439

Query: 547 FVTMPGCSPFSSRLHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGALLLVPTCLMAQ 606
           F+ +     FS+ +   +  ++ S  + +  + + ++S +NF+ + + A+LLVP   +  
Sbjct: 440 FIGI----IFSTFMTSEDAELVLSYDLMSKSLFISVVSTLNFSLSFVVAILLVPLQFI-- 493

Query: 607 PIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGLLGEGLVGLSLGGEFWTWLESL 666
               + R  R  SLL A     +LT     +F      +L   LV       FW W +  
Sbjct: 494 ----SFRFNRRLSLLFA-----VLTYFSTFIFLCSLSKILNGPLVP------FWLWAKEY 538

Query: 667 WAWKSATYLYIGMVH 681
             + S     + M+ 
Sbjct: 539 ELFNSWLMPSVFMIL 553
>ref|NP_013189.1| (NC_001144) ER protein essential for attaching GPI
           (glycosylphosphatidylinositol) to protein; Gaa1p
           [Saccharomyces cerevisiae]
 sp|P39012|GAA1_YEAST GAA1 PROTEIN
 pir||S45053 membrane protein END2 - yeast (Saccharomyces cerevisiae)
 emb|CAA55944.1| (X79409) Gaa1 [Saccharomyces cerevisiae]
 gb|AAB67592.1| (U53880) Gaa1p [Saccharomyces cerevisiae]
 emb|CAA97649.1| (Z73260) ORF YLR088w [Saccharomyces cerevisiae]
          Length = 614

 Score =  381 bits (970), Expect = e-104
 Identities = 162/718 (22%), Positives = 273/718 (37%), Gaps = 174/718 (24%)

Query: 24  RPIVRLGI--FLIAHSPVFSVVFSAAGVLALLLLPLLAK--NTYISENALMPGSARSMLS 79
           R IV +G+   +IA  PV S++ +  G +++ +LP+  +   TYISENALMP  A S   
Sbjct: 9   RRIVDMGLVPRIIALLPVISMLCALFGFISIAILPMDGQYRRTYISENALMPSQAYSYFR 68

Query: 80  NRDVSDGSKLVKDIKNFRLNHEGQVQKLIGKYMSDMGAEVSYQKFHPEGNQFHPLHFFSG 139
             + +        IK        +   L+G ++ + G + +                   
Sbjct: 69  ESEWNILRGYRSQIKEMVNMTSMERNNLMGSWLQEFGTKTA------------------- 109

Query: 140 PDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGIVSSLF 199
                + EN     YG  + G++ APRGDG E++VL  P+ F +  ++      +  SL 
Sbjct: 110 -----IYEN---EQYGETLYGVMHAPRGDGTEAMVLAVPW-FNSDDEFNIGGAALGVSLA 160

Query: 200 SLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCDEQNTADNFRRA 259
              SR    SK+II + +++     R   +W+  YH+ S  ++                 
Sbjct: 161 RFFSRWPVWSKNIIVVFSENPRAALR---SWVEAYHT-SLDLTG---------------- 200

Query: 260 GTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVNYLAVHRQGFYVKVEKVV 319
           G++ AA+VL     +E F + + I  +  NG++PNLDL+N+   +  H  G  V +  + 
Sbjct: 201 GSIEAAVVLDYSS-TEDFFEYVEISYDGLNGELPNLDLVNIAISITEHE-GMKVSLHGLP 258

Query: 320 SLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEGSATLASSLYSQALGIPTGP 379
           S                               +   ++      L   +   AL     P
Sbjct: 259 S-----------------------------DQLTNNNFWSRLKILCLGIRDWALSGVKKP 289

Query: 380 HG--AFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARLLEGTIRSVNNLLEKFHQSFF 437
           HG  AF  +++ ++TLK             HD      R+ E   RS+NNLLEKFHQSFF
Sbjct: 290 HGNEAFSGWRIQSVTLKA-------HGNSGHDITTF-GRIPEAMFRSINNLLEKFHQSFF 341

Query: 438 LYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKSLTNSTHNPTENFKSWKWLD 497
            Y+L +P +F+S+  Y+ +   L     + + + +I+   +  +                
Sbjct: 342 FYLLLAPRQFVSISSYLPSAVALSIAFAISSLNAFINNAYANISL--------------- 386

Query: 498 AAKQVFTLHLLGFIVTLLPYFICQVPGQHSPTNRSIMWGTTSSSLLLITFVTMPGCSPFS 557
            ++      L+ F+  ++ + + Q       +   +     S  L LI    +    P S
Sbjct: 387 FSEYNLVALLVWFVSLVISFVVSQAFLLIPSSGLLMTISMASCFLPLILSRKIHISEPLS 446

Query: 558 SRLHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGALLLVPTC--------------- 602
            R        LK+V      +    + +INFA A +   L  P                 
Sbjct: 447 YR--------LKNVAFLYFSLVSTSLLMINFAMALLIGTLAFPMTFVKTIVESSSEHEVT 498

Query: 603 ------LMAQPIK---------------PALRSRRLKSLLGAFCSIVLLTIGFPVMFFAI 641
                 +  +P                 P  + ++LK+L       VLL +  P  F +I
Sbjct: 499 TQSSNPIKTEPKDEIELVENHMDTTPATPQQQKQKLKNL-------VLLILTNP--FISI 549

Query: 642 SKGLLGEGLVGLSLGGEFWTW-----LESLWA-WKSATYLYIGMVHLPCWLLCLCILF 693
           +         GL    EF  +     L S W   K  ++  + +  LPCWLL L   F
Sbjct: 550 TLF-------GLFFDDEFHGFDIINKLVSAWLDLKCWSWFVLCIGWLPCWLLILASSF 600
>gb|AAH08920.1|AAH08920 (BC008920) Unknown (protein for IMAGE:3633567) [Homo sapiens]
          Length = 353

 Score = 36.3 bits (83), Expect = 1.6
 Identities = 11/63 (17%), Positives = 26/63 (40%), Gaps = 6/63 (9%)

Query: 176 VTPYDFINGGDYEDLSLGIVSSLFSLLSRVT-----WLSKDIIWLVADSRYGDYRPVAAW 230
           V P+  + G D     + ++  L  L SR+        + ++++  +     +Y+    W
Sbjct: 20  VAPWLSL-GADSNGSGVSVLLELARLFSRLYTYKRTHAAYNLLFFASGGGKFNYQGTKRW 78

Query: 231 LTE 233
           L +
Sbjct: 79  LED 81
>ref|NP_391526.1| (NC_000964) similar to antibiotic resistance protein [Bacillus
           subtilis]
 pir||B70065 antibiotic resistance protein homolog ywoG - Bacillus subtilis
 emb|CAB05383.1| (Z82987) unknown similar to quinolon resistance protein NorA
           [Bacillus subtilis]
 emb|CAB15662.1| (Z99122) similar to antibiotic resistance protein [Bacillus
           subtilis]
          Length = 396

 Score = 35.1 bits (80), Expect = 3.5
 Identities = 40/173 (23%), Positives = 59/173 (33%), Gaps = 26/173 (15%)

Query: 498 AAKQVFTLHLLGFIVTLLPYFICQVPGQHSPTNRSIMWGTTSSSLLLITFVTMPGCSPFS 557
               +F        +T+LP +  Q  G           G   S  LL   +T P      
Sbjct: 16  LLVNLFVFVFFYTFLTVLPIYTLQELGGTESQ-----GGLLISLFLLSAIITRPFSGAIV 70

Query: 558 SRLHGTNWAILKSVTISAAFIGLCLMSIINFAT--------AEIGALLLVPTCLMAQPIK 609
            R  G     + S+ + A    L  M I NF+             ++L   T  +A  I 
Sbjct: 71  ERF-GKKRMAIVSMALFALSSFL-YMPIHNFSLLLGLRFFQGIWFSILTTVTGAIAADII 128

Query: 610 PALR----------SRRLKSLLGAFCSIVLL-TIGFPVMFFAISKGLLGEGLV 651
           PA R          S  L   +G F  + L+  + FPV F A +  ++   LV
Sbjct: 129 PAKRRGEGLGYFAMSMNLAMAIGPFLGLNLMRVVSFPVFFTAFALFMVAGLLV 181
CPU time:   116.02 user secs.	    2.29 sys. secs	  118.31 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.313    0.153    0.430 

Gapped
Lambda     K      H
   0.270   0.0536    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 294867906
Number of Sequences: 887402
Number of extensions: 9639190
Number of successful extensions: 41860
Number of sequences better than 10.0: 105
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 93
Number of HSP's that attempted gapping in prelim test: 41711
Number of HSP's gapped (non-prelim): 138
length of query: 696
length of database: 277,845,442
effective HSP length: 56
effective length of query: 640
effective length of database: 228,150,930
effective search space: 146016595200
effective search space used: 146016595200
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.2 bits)
S2: 76 (33.8 bits)