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GPI Anchor Biosynthesis Report: NP_003792.1 (GAA1 family, Homo sapiens)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (621 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 3

Distribution of 14 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mu... 835 0.0
ref|NP_003792.1| (NM_003801) anchor attachment protein 1; anchor... 805 0.0
gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melano... 713 0.0
ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans... 702 0.0
pir||T46923 hypothetical protein DKFZp434E1512.1 - human >gi|701... 647 0.0
pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans 642 0.0
ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila mel... 639 0.0
ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis t... 620 e-176
emb|CAB65611.1| (AL136078) putative glycosylphosphatidylinositol... 559 e-158
ref|NP_013189.1| (NC_001144) ER protein essential for attaching ... 517 e-145
emb|CAB86709.1| (AL163505) hypothetical protein L3640.14 [Leishm... 299 8e-80
ref|NP_491701.1| (NM_059300) F33D11.9a.p [Caenorhabditis elegans... 185 2e-45
Sequences with E-value WORSE than threshold

gb|AAC96071.1| (AF038948) ultraviolet cone visual pigment; rhodo... 39 0.19
emb|CAB99396.1| (AL390355) phosphatidate cytidylyltransferase [S... 37 1.1
Alignments
>ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mus musculus]
 dbj|BAA82589.1| (AB002136) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAA82591.1| (AB002138) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAB03274.1| (AB006970) GPI anchor attachment protein [Mus musculus]
 dbj|BAB03275.1| (AB006971) GPI anchor attachment protein [Mus musculus]
 gb|AAH06697.1|AAH06697 (BC006697) GPI anchor attachment protein 1 [Mus musculus]
          Length = 621

 Score =  835 bits (2136), Expect = 0.0
 Identities = 567/621 (91%), Positives = 588/621 (94%)

Query: 1   MGLLSDPVRRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGSTMV 60
           MGLLSDPVRRRALAR+VLRLN PLCVLSYVAGIAWFLAL FPPLTQRTYMSENAMGSTMV
Sbjct: 1   MGLLSDPVRRRALARIVLRLNTPLCVLSYVAGIAWFLALAFPPLTQRTYMSENAMGSTMV 60

Query: 61  EEQFAGGDRARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHE 120
           EEQF GGDRAR+FARDFAAHRKK GALPVAWLER+MRSVGLEVYTQSFSRKLPFPDETHE
Sbjct: 61  EEQFVGGDRARSFARDFAAHRKKPGALPVAWLERSMRSVGLEVYTQSFSRKLPFPDETHE 120

Query: 121 RYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDI 180
           RYMVSGTNVYGILRAPR+ASTESLVLTVPCG D+TNSQAVGLLLALAAHFRGQIYWAKDI
Sbjct: 121 RYMVSGTNVYGILRAPRSASTESLVLTVPCGPDATNSQAVGLLLALAAHFRGQIYWAKDI 180

Query: 181 VFLVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVE 240
           +FLVT+HDLLGTEAWLEAYHD+NVTG+QSSPLQGRAGAIQAAVALELSSDVVTSLDV VE
Sbjct: 181 IFLVTDHDLLGTEAWLEAYHDINVTGIQSSPLQGRAGAIQAAVALELSSDVVTSLDVTVE 240

Query: 241 GLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVLRQASG 300
           GLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQP+DWTSL+GPLQGLQTLLLMVLRQASG
Sbjct: 241 GLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPQDWTSLEGPLQGLQTLLLMVLRQASG 300

Query: 301 RPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQSFFLY 360
           RPHG HGLFLRY VEALTLRGINSFRQYKYDL  VGKALEGMFRKLNHLLERLHQSFF Y
Sbjct: 301 RPHGPHGLFLRYGVEALTLRGINSFRQYKYDLATVGKALEGMFRKLNHLLERLHQSFFFY 360

Query: 361 LLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPPSQ 420
           LLP LSRFVSIGLYMPA GFLLLVLGLKALELWMQLH+AG+  EE G AP P  PL P+Q
Sbjct: 361 LLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQLHQAGVNPEEAGKAPSPGTPLLPTQ 420

Query: 421 GVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPH 480
           GVGLASL APLLISQAMGLALY LPVLGQH+ATQHFPVAEAEAVVLTLLAIY AGLALPH
Sbjct: 421 GVGLASLTAPLLISQAMGLALYFLPVLGQHLATQHFPVAEAEAVVLTLLAIYVAGLALPH 480

Query: 481 NTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKPHGP 540
           NTHRVV++Q PDRGWMALKLVALIYLALQLGCIAL NFSLGFLLA TMVP AALAKPHGP
Sbjct: 481 NTHRVVNSQVPDRGWMALKLVALIYLALQLGCIALLNFSLGFLLAATMVPAAALAKPHGP 540

Query: 541 RTLYAALLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLF 600
           RTLYAALLV+TSPA TL GSLFLWREL E PLSLAEGWQLFL ALAQGVLEH+TYGALLF
Sbjct: 541 RTLYAALLVVTSPAVTLFGSLFLWRELLEVPLSLAEGWQLFLTALAQGVLEHYTYGALLF 600

Query: 601 PLLSLGLYPCWLLFWNVLFWK 621
           P+L+LGLYPCWLLFWNVLFWK
Sbjct: 601 PILALGLYPCWLLFWNVLFWK 621
>ref|NP_003792.1| (NM_003801) anchor attachment protein 1; anchor attachment protein
           1 (Gaa1p, yeast) homolog; glycophosphatidylinositol
           anchor attachment 1 [Homo sapiens]
 dbj|BAA24035.1| (AB006969) hGAA1 [Homo sapiens]
 dbj|BAA82588.1| (AB002135) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Homo sapiens]
 dbj|BAA82590.1| (AB002137) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Homo sapiens]
 gb|AAH03171.1|AAH03171 (BC003171) anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo
           sapiens]
 gb|AAH04129.1|AAH04129 (BC004129) anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo
           sapiens]
          Length = 621

 Score =  805 bits (2059), Expect = 0.0
 Identities = 621/621 (100%), Positives = 621/621 (100%)

Query: 1   MGLLSDPVRRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGSTMV 60
           MGLLSDPVRRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGSTMV
Sbjct: 1   MGLLSDPVRRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGSTMV 60

Query: 61  EEQFAGGDRARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHE 120
           EEQFAGGDRARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHE
Sbjct: 61  EEQFAGGDRARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHE 120

Query: 121 RYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDI 180
           RYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDI
Sbjct: 121 RYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDI 180

Query: 181 VFLVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVE 240
           VFLVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVE
Sbjct: 181 VFLVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVE 240

Query: 241 GLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVLRQASG 300
           GLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVLRQASG
Sbjct: 241 GLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVLRQASG 300

Query: 301 RPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQSFFLY 360
           RPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQSFFLY
Sbjct: 301 RPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQSFFLY 360

Query: 361 LLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPPSQ 420
           LLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPPSQ
Sbjct: 361 LLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPPSQ 420

Query: 421 GVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPH 480
           GVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPH
Sbjct: 421 GVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPH 480

Query: 481 NTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKPHGP 540
           NTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKPHGP
Sbjct: 481 NTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKPHGP 540

Query: 541 RTLYAALLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLF 600
           RTLYAALLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLF
Sbjct: 541 RTLYAALLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLF 600

Query: 601 PLLSLGLYPCWLLFWNVLFWK 621
           PLLSLGLYPCWLLFWNVLFWK
Sbjct: 601 PLLSLGLYPCWLLFWNVLFWK 621
>gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melanogaster]
          Length = 674

 Score =  713 bits (1822), Expect = 0.0
 Identities = 220/662 (33%), Positives = 311/662 (46%), Gaps = 64/662 (9%)

Query: 1   MGLLSDP--VRRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGST 58
           MGLLSDP    +  L   + R    +C   YVAG+AWF  L  P     TY+SENA+   
Sbjct: 1   MGLLSDPSISTQSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPG 60

Query: 59  MVEE--QFAGGDRARAFARDFAAHRKKS-GALPVAWLERTMRSVGLEVYTQSFSRKLPF- 114
           +V    +      A     +    RK      P AW+   M   GLE +T +++ + PF 
Sbjct: 61  LVYPEIRIDANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFG 120

Query: 115 -PDETHERYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTN----SQAVGLLLALAAH 169
              E H      G N+YGILRAPR ASTE +V   P  + S+     S +V LLLA A  
Sbjct: 121 GGKEYH------GKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADF 174

Query: 170 FRGQIYWAKDIVFLVTEHDLLGTEAWLEAYH------DVNVTGMQSSPLQGRAGAIQAAV 223
            R + YWAKD++FL+TE + LG +AWLEAYH      D++   ++   L  RAG++QAA+
Sbjct: 175 ARRKNYWAKDLIFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAAL 234

Query: 224 ALELSSDVVTSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGP 283
            +E+    +  +DV +EGLNG+LPNLD+ NL Q    + G+    +   + +   S    
Sbjct: 235 NIEVQDLEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHF 294

Query: 284 LQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTL----RGINSFRQYKYDLVAVG--K 337
            Q  + +L M+  Q+SG P G+HGLF RYR++ALT+    R  ++  +      AV   K
Sbjct: 295 EQNFRQMLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLK 354

Query: 338 ALEGMFRKLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLH 397
           A+EG+ R LN+LLER HQSFF Y++    R++SIG YMPA+  L+    LKA   W  L 
Sbjct: 355 AIEGIARSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLP 414

Query: 398 EAGMGLEEPGG-------APGPSVPLPPSQ-----GVGLASLVAPLLISQAMGLALYVLP 445
                LE+  G              L P +     G  L  L A LLI    G    VLP
Sbjct: 415 ATNAELEKAAGWLREHEQEAELEENLEPDKFELPYGSVLIYLTATLLI----GFLCNVLP 470

Query: 446 VLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIY 505
            L Q+        A     VL+ L+    G  LP           P  G   L +  L+ 
Sbjct: 471 -LQQYFLEIPMGAAPLTTSVLSFLS--LIGFVLP------FVVVLPPGGLELLHVAFLLI 521

Query: 506 LALQLGCIALTNFSLGFLLATTMVP--TAALAKPHGPR-----TLYAALLVLTSPAATLL 558
               L  I L NF+LG   A   VP   A   K    R     T+  A LV+       +
Sbjct: 522 YGCALIVIGLLNFALGLFAAVLTVPLVIALETKEENSRSTLRNTIRLATLVMNPMMVVYV 581

Query: 559 GSLFL-WRELQEAPL--SLAEGWQLFLAALAQGVLEHHTYGALLFPLLSLGLYPCWLLFW 615
             L + + +  E P+   +       + A A G+++   YG  L+ ++     P W++ W
Sbjct: 582 IVLAMTFYQFPELPVQKIMLRAATAAMDASAYGLIDSVIYGNWLYFVICTIFLPLWIICW 641

Query: 616 NV 617
            +
Sbjct: 642 TL 643
>ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans]
 gb|AAK68360.1|AF039720_13 (AF039720) Hypothetical protein F33D11.9b [Caenorhabditis elegans]
          Length = 670

 Score =  702 bits (1794), Expect = 0.0
 Identities = 144/641 (22%), Positives = 253/641 (39%), Gaps = 75/641 (11%)

Query: 9   RRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGSTMVEEQFAGGD 68
            R  +   +L+      +++Y+        L  P   + T +SE+++   +V  +F    
Sbjct: 28  TRPKILDKILKFRKVALIIAYIGAYLSAQWLFDPVNMEVTRISEHSLMPGLVTPKFDKSG 87

Query: 69  RARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTN 128
            A    R F+   K      +  +      +GLE +T  +  K+           ++G N
Sbjct: 88  IAIQLYRRFSDLPKSKSQQEL--IHTIFSDLGLECFTHKWRSKVAGNP-------MNGEN 138

Query: 129 VYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVFLVT--- 185
           VYG +R PR    E+ ++ V  G    + + +  +LA   + + Q+YWA+DIV +     
Sbjct: 139 VYGFIRGPRNDGAEAQMIVVQLGRSEKSRRMMSRMLAFVDYAKDQVYWARDIVIVFVDGG 198

Query: 186 ------EHDLLGTEAWLEAYHDVNVTGMQSS------PLQGRAGAIQAAVALELSSDVVT 233
                 E      +A+L  Y  +     + S       +Q + GA+   V  +LS   V 
Sbjct: 199 EKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGALIGGVVYDLSGMAVK 258

Query: 234 S---LDVAVEGLNGQLPNLDLLNLFQTFC-----QKGGLLCTLQGKLQP-EDWTSLDGPL 284
               +++   GLNGQ  NLD+ N            +  +  TL     P  +++    P+
Sbjct: 259 GQHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHSRVAIFGTLHRHSNPYMEYSPFIVPM 318

Query: 285 QGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFR 344
           + L T       QA     G H +  +Y V+ LT+        + Y     G+ +E + R
Sbjct: 319 RALYT-------QAFISIEGIHSVLGKYGVQGLTVG-----LSHDYSEKQTGQFIEEVSR 366

Query: 345 KLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLE 404
            LN++LERLHQS+F+Y+L     F SI +Y+  +  L+  L + A   W ++ E      
Sbjct: 367 MLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQEFQF--- 423

Query: 405 EPGGAPGPSVPLPPSQGVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAV 464
                  P V L     +G    +A   + Q +G    VL  +                 
Sbjct: 424 -------PVVHLA-FHVLGFFYYIATTYVFQTIGTTF-VLQWMQWPFVGID-GCDGQFTP 473

Query: 465 VLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLL 524
             +L+  +     +P   +  V  + P R   ++++V L+  +L L  I LTNF L  L 
Sbjct: 474 SWSLINSFLIFFMIPLGPY--VFLKEPVRCVPSMRIVCLLETSLVLYGICLTNFGLAILC 531

Query: 525 ATTMVPTAALAKPHGPRTLY--AALLVLTSPAATLLGS---LFLWRELQEAPLSLAEGWQ 579
              +VP   L      R+++  + L +L   +     +     ++ +L   P    + W 
Sbjct: 532 TAFVVPIVFL---MTTRSIFRSSVLAILNPISVLAFVAINIPGMFGQL--FPTISQDSWL 586

Query: 580 LFLAALAQGVL-EHHTYGALLFPLLSLGLYPCWLLFWNVLF 619
               ++A   + E   +    F LL    YP     WN+LF
Sbjct: 587 AAAQSIALTAIREDVEFKVNHFFLLCCFGYPI----WNMLF 623
>pir||T46923 hypothetical protein DKFZp434E1512.1 - human
 emb|CAB75660.1| (AL157437) hypothetical protein [Homo sapiens]
 gb|AAH06383.1|AAH06383 (BC006383) Unknown (protein for MGC:12663) [Homo sapiens]
          Length = 499

 Score =  647 bits (1652), Expect = 0.0
 Identities = 499/499 (100%), Positives = 499/499 (100%)

Query: 123 MVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVF 182
           MVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVF
Sbjct: 1   MVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVF 60

Query: 183 LVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVEGL 242
           LVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVEGL
Sbjct: 61  LVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVEGL 120

Query: 243 NGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVLRQASGRP 302
           NGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVLRQASGRP
Sbjct: 121 NGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVLRQASGRP 180

Query: 303 HGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQSFFLYLL 362
           HGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQSFFLYLL
Sbjct: 181 HGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQSFFLYLL 240

Query: 363 PGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPPSQGV 422
           PGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPPSQGV
Sbjct: 241 PGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPPSQGV 300

Query: 423 GLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPHNT 482
           GLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPHNT
Sbjct: 301 GLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPHNT 360

Query: 483 HRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKPHGPRT 542
           HRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKPHGPRT
Sbjct: 361 HRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKPHGPRT 420

Query: 543 LYAALLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLFPL 602
           LYAALLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLFPL
Sbjct: 421 LYAALLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLFPL 480

Query: 603 LSLGLYPCWLLFWNVLFWK 621
           LSLGLYPCWLLFWNVLFWK
Sbjct: 481 LSLGLYPCWLLFWNVLFWK 499
>pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans
          Length = 885

 Score =  642 bits (1639), Expect = 0.0
 Identities = 129/641 (20%), Positives = 230/641 (35%), Gaps = 122/641 (19%)

Query: 9   RRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGSTMVEEQFAGGD 68
            R  +   +L+      +++Y+        L  P   + T +SE+++   +V  +F    
Sbjct: 290 TRPKILDKILKFRKVALIIAYIGAYLSAQWLFDPVNMEVTRISEHSLMPGLVTPKFDKSG 349

Query: 69  RARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTN 128
            A    R F+   K      +  +      +GLE +T  +  K+           ++G N
Sbjct: 350 IAIQLYRRFSDLPKSKSQQEL--IHTIFSDLGLECFTHKWRSKVAGNP-------MNGEN 400

Query: 129 VYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVFLVT--- 185
           VYG +R PR    E+ ++ V  G    + + +  +LA   + + Q+YWA+DIV +     
Sbjct: 401 VYGFIRGPRNDGAEAQMIVVQLGRSEKSRRMMSRMLAFVDYAKDQVYWARDIVIVFVDGG 460

Query: 186 ------EHDLLGTEAWLEAYHDVNVTGMQSS------PLQGRAGAIQAAVALELSSDVVT 233
                 E      +A+L  Y  +     + S       +Q + GA+   V  +LS   V 
Sbjct: 461 EKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGALIGGVVYDLSGMAVK 520

Query: 234 S---LDVAVEGLNGQLPNLDLLNLFQTFC-----QKGGLLCTLQGKLQP-EDWTSLDGPL 284
               +++   GLNGQ  NLD+ N            +  +  TL     P  +++    P+
Sbjct: 521 GQHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHSRVAIFGTLHRHSNPYMEYSPFIVPM 580

Query: 285 QGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFR 344
           + L T       QA     G H +  +Y V+ LT+        + Y     G+ +E + R
Sbjct: 581 RALYT-------QAFISIEGIHSVLGKYGVQGLTVG-----LSHDYSEKQTGQFIEEVSR 628

Query: 345 KLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLE 404
            LN++LERLHQS+F+Y+L     F SI +Y+  +  L+  L + A   W ++ E      
Sbjct: 629 MLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQEFQF--- 685

Query: 405 EPGGAPGPSVPLPPSQGVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAV 464
                  P V L     +G    +A   + Q +G    VL  +                 
Sbjct: 686 -------PVVHLA-FHVLGFFYYIATTYVFQTIGTTF-VLQWMQWPFVGID-GCDGQFTP 735

Query: 465 VLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLL 524
             +L+  +     +P   +                 V L                     
Sbjct: 736 SWSLINSFLIFFMIPLGPY-----------------VFL--------------------- 757

Query: 525 ATTMVPTAALAKPHGPRTLY--AALLVLTSPAATLLGS---LFLWRELQEAPLSLAEGWQ 579
                           R+++  + L +L   +     +     ++ +L   P    + W 
Sbjct: 758 --------------KTRSIFRSSVLAILNPISVLAFVAINIPGMFGQL--FPTISQDSWL 801

Query: 580 LFLAALAQGVL-EHHTYGALLFPLLSLGLYPCWLLFWNVLF 619
               ++A   + E   +    F LL    YP     WN+LF
Sbjct: 802 AAAQSIALTAIREDVEFKVNHFFLLCCFGYPI----WNMLF 838
>ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila melanogaster]
 gb|AAK77275.1| (AY047543) GH05723p [Drosophila melanogaster]
          Length = 561

 Score =  639 bits (1632), Expect = 0.0
 Identities = 203/577 (35%), Positives = 276/577 (47%), Gaps = 56/577 (9%)

Query: 1   MGLLSDP--VRRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGST 58
           MGLLSDP    +  L   + R    +C   YVAG+AWF  L  P     TY+SENA+   
Sbjct: 1   MGLLSDPSISTQSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPG 60

Query: 59  MVEE--QFAGGDRARAFARDFAAHRKKS-GALPVAWLERTMRSVGLEVYTQSFSRKLPF- 114
           +V    +      A     +    RK      P AW+   M   GLE +T +++ + PF 
Sbjct: 61  LVYPEIRIDANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFG 120

Query: 115 -PDETHERYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTN----SQAVGLLLALAAH 169
              E H      G N+YGILRAPR ASTE +V   P  + S+     S +V LLLA A  
Sbjct: 121 GGKEYH------GKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADF 174

Query: 170 FRGQIYWAKDIVFLVTEHDLLGTEAWLEAYH------DVNVTGMQSSPLQGRAGAIQAAV 223
            R + YWAKD++FL+TE + LG +AWLEAYH      D++   ++   L  RAG++QAA+
Sbjct: 175 ARRKNYWAKDLIFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAAL 234

Query: 224 ALELSSDVVTSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGP 283
            +E+    +  +DV +EGLNG+LPNLD+ NL Q    + G+    +   + +   S    
Sbjct: 235 NIEVQDLEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHF 294

Query: 284 LQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTL----RGINSFRQYKYDLVAVG--K 337
            Q  + +L M+  Q+SG P G+HGLF RYR++ALT+    R  ++  +      AV   K
Sbjct: 295 EQNFRQMLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLK 354

Query: 338 ALEGMFRKLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLH 397
           A+EG+ R LN+LLER HQSFF Y++    R++SIG YMPA+  L+    LKA   W  L 
Sbjct: 355 AIEGIARSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLP 414

Query: 398 EAGMGLEEPGG-------APGPSVPLPPSQ-----GVGLASLVAPLLISQAMGLALYVLP 445
                LE+  G              L P +     G  L  L A LLI    G    VLP
Sbjct: 415 ATNAELEKAAGWLREHEQEAELEENLEPDKFELPYGSVLIYLTATLLI----GFLCNVLP 470

Query: 446 VLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIY 505
            L Q+        A     VL+ L+    G  LP           P  G   L +  L+ 
Sbjct: 471 -LQQYFLEIPMGAAPLTTSVLSFLS--LIGFVLP------FVVVLPPGGLELLHVAFLLI 521

Query: 506 LALQLGCIALTNFSLGFLLATTMVP--TAALAKPHGP 540
               L  I L NF+LG   A   VP   A   K    
Sbjct: 522 YGCALIVIGLLNFALGLFAAVLTVPLVIALETKEENS 558
>ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis thaliana]
          Length = 696

 Score =  620 bits (1584), Expect = e-176
 Identities = 176/696 (25%), Positives = 279/696 (39%), Gaps = 101/696 (14%)

Query: 5   SDPVRRRALARLVLRLNAPLCVLSYV-AGIAWFLALVFPPLTQRTYMSENAMGSTMVEEQ 63
           S  ++ R + RL + L A   V S V +       L+ P L + TY+SENA+        
Sbjct: 18  SPKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSM 77

Query: 64  FAGGDRARA--FARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHER 121
            +  D +      +D    R          + + M  +G EV  Q F    P  ++ H  
Sbjct: 78  LSNRDVSDGSKLVKDIKNFRLNHEGQVQKLIGKYMSDMGAEVSYQKFH---PEGNQFHPL 134

Query: 122 YMVS----------------GTNVYGILRAPRAASTESLVLTVPC----GSDSTNSQAVG 161
           +  S                G NV GI+RAPR    ES+VL  P     G D  +  ++G
Sbjct: 135 HFFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYED-LSLG 193

Query: 162 LLLALAAHFRGQIYWAKDIVFLVTEH---DLLGTEAWLEAYH--DVNVTGMQSSPLQG-- 214
           ++ +L +      + +KDI++LV +    D     AWL  YH     V+ +     Q   
Sbjct: 194 IVSSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCDEQNTA 253

Query: 215 ----RAGAIQAAVALEL---SSDVVTSLDVAVEGLNGQLPNLDLLNLF------------ 255
               RAG + AA+ L++   S     +L +  E  NGQ+PNLDL+N+             
Sbjct: 254 DNFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVNYLAVHRQGFYV 313

Query: 256 --------------QTFCQKGGLLCTLQGKLQPEDWT---SLDGPLQGLQTLLLMVLRQA 298
                         + F +    +  L   L P DW         L+G  TL   +  QA
Sbjct: 314 KVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNP-DWNFGIPAADYLEGSATLASSLYSQA 372

Query: 299 SGRPHGSHGLFLRYRVEALTLRGINSF------RQYKYDLVAVGKALEGMFRKLNHLLER 352
            G P G HG F  Y+V+A+TL+    F      RQ+ + L    + LEG  R +N+LLE+
Sbjct: 373 LGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRG-ARLLEGTIRSVNNLLEK 431

Query: 353 LHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGP 412
            HQSFFLY+L   S+F+S+G+YM A   L+  L + A  L++   ++            P
Sbjct: 432 FHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKS-----LTNSTHNP 486

Query: 413 SVPLPPSQGVGLASLVAPLLISQAMGLALYVLP-----VLGQHVATQHFPVAEAEAVVLT 467
           +      + +  A  V  L     +G  + +LP     V GQH  T    +    +  L 
Sbjct: 487 TENFKSWKWLDAAKQVFTL---HLLGFIVTLLPYFICQVPGQHSPTNRSIMWGTTSSSLL 543

Query: 468 LLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATT 527
           L+         P       S++     W  LK V +    + L  +++ NF+   + A  
Sbjct: 544 LITFVTMPGCSP------FSSRLHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGALL 597

Query: 528 MVPTAALAKPHGP----RTLYAALLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLA 583
           +VPT  +A+P  P    R L + L    S     +G   ++  + +  L           
Sbjct: 598 LVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGLLGEGLVGLSLGG 657

Query: 584 ALAQGVLEHHTYGALLFPLLSLGLYPCWLLFWNVLF 619
                +     + +  +  + +   PCWLL   +LF
Sbjct: 658 EFWTWLESLWAWKSATYLYIGMVHLPCWLLCLCILF 693
>emb|CAB65611.1| (AL136078) putative glycosylphosphatidylinositol anchor attachment
           protein [Schizosaccharomyces pombe]
          Length = 581

 Score =  559 bits (1427), Expect = e-158
 Identities = 156/610 (25%), Positives = 257/610 (41%), Gaps = 81/610 (13%)

Query: 14  ARLVLRLNAPLCVL---SYVAGIAWFLALVFPPLTQRTYMSENAMGSTMV----EEQFAG 66
            R+   L   L  L     + G++W   L    +  R ++SE+A+    V    E +++ 
Sbjct: 9   IRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFENRYSK 68

Query: 67  GDRARAFARDFAAHRKKSGALPVAW--LERTMRSVGLEVYTQSFSRKLPFPDETHERYMV 124
              +   A +  +H   S      +  LE+   ++GL    Q++S  +P  +        
Sbjct: 69  TVSSSLTAANTWSHLDASVGTNTMYDDLEQIFTAMGLPTQKQNYSINIPGSE-------F 121

Query: 125 SGTNVYGILRAPRAASTESLVLTVPCGSD--STNSQAVGLLLALAAHFRGQIYWAKDIVF 182
           +G+N    LRAPR  +TESL+L VP        N   V L ++L  +F+G   W+KDI+ 
Sbjct: 122 NGSNFITTLRAPRGDATESLLLCVPWKDHIGQYNEAGVALAISLLKYFQGWSLWSKDIIL 181

Query: 183 LVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALEL--SSDVVTSLDVAVE 240
           ++ +  + G  ++L +Y D     +  +PL+ R+G+IQA ++LEL  + +    L+V  +
Sbjct: 182 VIFDDPVYGPSSFLTSYFDQTTPYISYTPLKIRSGSIQAGLSLELVTTENNSDVLEVLYQ 241

Query: 241 GLNGQLPNLDLLN-LFQTFCQKGGLLCTLQGKLQPEDWTSLDG--PLQGLQTLLLMVLRQ 297
             NGQLPNLDL N + + F Q       LQG     D+ +  G      L++L + +L Q
Sbjct: 242 ATNGQLPNLDLFNTISRIFMQHFNYPLRLQGY----DFHANSGSSYTSRLKSLWMGMLTQ 297

Query: 298 ASGRPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQSF 357
           A      +H LF +YR++ LTLR +     + +D+   G+A+E  FR LN+LLE LHQSF
Sbjct: 298 AVSNVTSAHALFPQYRIDMLTLR-MKVKDPFSFDMFRFGQAIESTFRSLNNLLEHLHQSF 356

Query: 358 FLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLP 417
           F Y +     F+SIG YMP++  L     L A   W+  HE  + L  P           
Sbjct: 357 FFYFILDHLHFISIGNYMPSILILAASFMLGAYRHWIN-HEKKIDLWRPFSFWL----FS 411

Query: 418 PSQGVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAIYAAGLA 477
               +    LV+             +L  +G   +T               +    A L 
Sbjct: 412 IFCTIAAYYLVSSSTKITVFIFLYLMLTFIGIIFST--------------FMTSEDAELV 457

Query: 478 LPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKP 537
           L ++                         +L +  ++  NFSL F++A  +VP   ++  
Sbjct: 458 LSYDLMSK---------------------SLFISVVSTLNFSLSFVVAILLVPLQFISFR 496

Query: 538 HGPRTLYAALLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGA 597
              R L     VLT  +      L    ++   PL                  E+  + +
Sbjct: 497 FN-RRLSLLFAVLTYFS--TFIFLCSLSKILNGPLV----------PFWLWAKEYELFNS 543

Query: 598 LLFPLLSLGL 607
            L P + + L
Sbjct: 544 WLMPSVFMIL 553
>ref|NP_013189.1| (NC_001144) ER protein essential for attaching GPI
           (glycosylphosphatidylinositol) to protein; Gaa1p
           [Saccharomyces cerevisiae]
 sp|P39012|GAA1_YEAST GAA1 PROTEIN
 pir||S45053 membrane protein END2 - yeast (Saccharomyces cerevisiae)
 emb|CAA55944.1| (X79409) Gaa1 [Saccharomyces cerevisiae]
 gb|AAB67592.1| (U53880) Gaa1p [Saccharomyces cerevisiae]
 emb|CAA97649.1| (Z73260) ORF YLR088w [Saccharomyces cerevisiae]
          Length = 614

 Score =  517 bits (1318), Expect = e-145
 Identities = 148/544 (27%), Positives = 245/544 (44%), Gaps = 71/544 (13%)

Query: 1   MGLLSDPVRRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLT---QRTYMSENAMGS 57
           M LL    RR     LV R+ A L V+S +  +  F+++   P+    +RTY+SENA+  
Sbjct: 1   MALLEKLHRRIVDMGLVPRIIALLPVISMLCALFGFISIAILPMDGQYRRTYISENALMP 60

Query: 58  TMVEEQFAGGDRARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDE 117
           +     F   +      R + +  K      +  +    R+  +  + Q F  K    + 
Sbjct: 61  SQAYSYF--RESEWNILRGYRSQIK-----EMVNMTSMERNNLMGSWLQEFGTKTAIYE- 112

Query: 118 THERYMVSGTNVYGILRAPRAASTESLVLTVPC--GSDSTNSQAVGLLLALAAHFRGQIY 175
            +E+Y   G  +YG++ APR   TE++VL VP     D  N     L ++LA  F     
Sbjct: 113 -NEQY---GETLYGVMHAPRGDGTEAMVLAVPWFNSDDEFNIGGAALGVSLARFFSRWPV 168

Query: 176 WAKDIVFLVTEHDLLGTEAWLEAYH-DVNVTGMQSSPLQGRAGAIQAAVALELS--SDVV 232
           W+K+I+ + +E+      +W+EAYH  +++TG          G+I+AAV L+ S   D  
Sbjct: 169 WSKNIIVVFSENPRAALRSWVEAYHTSLDLTG----------GSIEAAVVLDYSSTEDFF 218

Query: 233 TSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLL 292
             ++++ +GLNG+LPNLDL+N+  +  +  G+  +L G   P D  + +     L+ L L
Sbjct: 219 EYVEISYDGLNGELPNLDLVNIAISITEHEGMKVSLHG--LPSDQLTNNNFWSRLKILCL 276

Query: 293 MVLRQASG---RPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHL 349
            +   A     +PHG+   F  +R++++TL+   +     +D+   G+  E MFR +N+L
Sbjct: 277 GIRDWALSGVKKPHGNEA-FSGWRIQSVTLK---AHGNSGHDITTFGRIPEAMFRSINNL 332

Query: 350 LERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGA 409
           LE+ HQSFF YLL    +FVSI  Y+P+   L +   + +L  ++    A + L      
Sbjct: 333 LEKFHQSFFFYLLLAPRQFVSISSYLPSAVALSIAFAISSLNAFINNAYANISL------ 386

Query: 410 PGPSVPLPPSQGVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLL 469
                       V L      L+IS  +  A  ++P  G                   L+
Sbjct: 387 ------FSEYNLVALLVWFVSLVISFVVSQAFLLIPSSG------------------LLM 422

Query: 470 AIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMV 529
            I  A   LP    R +    P      LK VA +Y +L    + + NF++  L+ T   
Sbjct: 423 TISMASCFLPLILSRKIHISEPLS--YRLKNVAFLYFSLVSTSLLMINFAMALLIGTLAF 480

Query: 530 PTAA 533
           P   
Sbjct: 481 PMTF 484
>emb|CAB86709.1| (AL163505) hypothetical protein L3640.14 [Leishmania major]
          Length = 464

 Score =  299 bits (760), Expect = 8e-80
 Identities = 54/318 (16%), Positives = 94/318 (28%), Gaps = 40/318 (12%)

Query: 134 RAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVFLVTEHDLLGTE 193
           R  R+  +E  +  V   +D     +V L  AL    R +   A D+ F           
Sbjct: 75  RGRRSPGSE--IAAVYVNTDY--PASVSLANALIEVIRRRKSLACDVQFYFVNS------ 124

Query: 194 AWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVEGLNGQLPNLDLLN 253
                          S          +AA+ L LS+     + +   G NG  PN D  N
Sbjct: 125 ---------------SEEWPVPQAYTRAALVLNLSTLYNRHICIDTLGGNGIQPNQDYTN 169

Query: 254 LFQTFCQKGGLLCTL---QGKLQPEDWTS-LDGPL---QGLQTLLLMVLRQASGRPHGSH 306
           +          L +    +     +D  +         +    L            H  +
Sbjct: 170 IAVQLAAANQFLPSYLCQRRHRSTDDAHAGFWHYWAYLEASVQLPTRPQPWHIIPTHSIN 229

Query: 307 GLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQSFFLYLLPGLS 366
            +     + +  L   +S   +     A  + +  +   LN L E+ H S F++L   L 
Sbjct: 230 TI----AISSDPLYSASSVFPHSQLPDAFAQLVLKIIVSLNGLEEKFHHSTFVWLPVSLW 285

Query: 367 RFVSIGLYMPAVGFLLLVLGLKALELW----MQLHEAGMGLEEPGGAPGPSVPLPPSQGV 422
           R+V        +   +  +       +    + +    + L     A   +  +     V
Sbjct: 286 RYVEYDHAQFCIILFVASIFSTTYSEYQLRGISITPLFVTLLLTPVAAAAAFQVAGWWAV 345

Query: 423 GLASLVAPLLISQAMGLA 440
             AS    LL    MG A
Sbjct: 346 VSASAAFSLLFRLLMGHA 363
>ref|NP_491701.1| (NM_059300) F33D11.9a.p [Caenorhabditis elegans]
 pir||T32772 hypothetical protein F33D11.9a - Caenorhabditis elegans
 gb|AAB96702.1| (AF039720) Hypothetical protein F33D11.9a [Caenorhabditis elegans]
          Length = 229

 Score =  185 bits (466), Expect = 2e-45
 Identities = 36/253 (14%), Positives = 69/253 (27%), Gaps = 77/253 (30%)

Query: 373 LYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPPSQGVGLASLVAPLL 432
           +Y+  +  L+  L + A   W ++ E             P V L     +G    +A   
Sbjct: 1   MYIIPLTILVSPLVISAYFEWTKIQEFQF----------PVVHLA-FHVLGFFYYIATTY 49

Query: 433 ISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPHNTHRVVSTQAPD 492
           + Q +G    VL  +                   +L+  +     +P   +         
Sbjct: 50  VFQTIGTTF-VLQWMQWPFVGID-GCDGQFTPSWSLINSFLIFFMIPLGPY--------- 98

Query: 493 RGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKPHGPRTLY--AALLVL 550
                   V L                                     R+++  + L +L
Sbjct: 99  --------VFL-----------------------------------KTRSIFRSSVLAIL 115

Query: 551 TSPAATLLGS---LFLWRELQEAPLSLAEGWQLFLAALAQGVL-EHHTYGALLFPLLSLG 606
              +     +     ++ +L   P    + W     ++A   + E   +    F LL   
Sbjct: 116 NPISVLAFVAINIPGMFGQL--FPTISQDSWLAAAQSIALTAIREDVEFKVNHFFLLCCF 173

Query: 607 LYPCWLLFWNVLF 619
            YP     WN+LF
Sbjct: 174 GYPI----WNMLF 182
>gb|AAC96071.1| (AF038948) ultraviolet cone visual pigment; rhodopsin [Ambystoma
           tigrinum]
          Length = 347

 Score = 39.2 bits (91), Expect = 0.19
 Identities = 34/211 (16%), Positives = 59/211 (27%), Gaps = 21/211 (9%)

Query: 412 PSVPLPPSQGVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAV-VLTLLA 470
           P   + P+         A       MG   +V   L   V        +        L+ 
Sbjct: 22  PQYHIAPAWT--FYFQTA------FMGFVFFVGTPLNAIVLIVTVKYKKLRQPLNYILVN 73

Query: 471 IYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVP 530
           +  AG      +   V   +    ++  K +  +   L      +T +SL FL     + 
Sbjct: 74  VSLAGFTFCIFSVFTVFVSSSQGYFIFGKTICELEAFLGSVSGLVTGWSLAFLAIERYIV 133

Query: 531 TAALAKPHGPRTLYAALLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLAALAQGV- 589
                KP G     +    +   A   +G       L      + EG Q         V 
Sbjct: 134 IC---KPMGSFRFSSKHATMVVLATWAIGFSVSIGPLVGWSRYIPEGLQCSCGPDWYTVG 190

Query: 590 ----LEHHTYGALLFPLLSLGLYPCWLLFWN 616
                E +T+    F  +   + P  L+ + 
Sbjct: 191 TKYNSETYTW----FLFIFCFIIPLSLICFC 217
>emb|CAB99396.1| (AL390355) phosphatidate cytidylyltransferase [Schizosaccharomyces
           pombe]
          Length = 439

 Score = 36.9 bits (85), Expect = 1.1
 Identities = 26/163 (15%), Positives = 44/163 (26%), Gaps = 12/163 (7%)

Query: 353 LHQSF----FLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKAL-ELWMQLHEAGMGLEEPG 407
            H  F    F+  L    RF+S  LY+      +  L        + Q     M L    
Sbjct: 132 FHHLFIMDSFMLPLVLHHRFISFMLYIIGFVLFVASLKKGNYKFQFSQFCWTHMTLLLVV 191

Query: 408 GAPGPSVP--LPP--SQGVGLASLVAPLLISQAMGLAL---YVLPVLGQHVATQHFPVAE 460
           G     +           V +  +V   + +   G       ++ V  +           
Sbjct: 192 GQSHFMINNLFEGLFWFFVPVCYVVCNDVFAYLCGKMFGKHPLIQVSPKKTVEGFLGGWI 251

Query: 461 AEAVVLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVAL 503
              V+ +L++             R +ST A          V L
Sbjct: 252 CTVVIGSLISYVLMHFKYFICPTRDLSTSAFSGLNCTPNSVFL 294
CPU time:   152.42 user secs.	    2.65 sys. secs	  155.07 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.316    0.172    0.471 

Gapped
Lambda     K      H
   0.270   0.0604    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 357721767
Number of Sequences: 887402
Number of extensions: 16382795
Number of successful extensions: 75236
Number of sequences better than 10.0: 499
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 489
Number of HSP's that attempted gapping in prelim test: 74866
Number of HSP's gapped (non-prelim): 670
length of query: 621
length of database: 277,845,442
effective HSP length: 56
effective length of query: 565
effective length of database: 228,150,930
effective search space: 128905275450
effective search space used: 128905275450
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.8 bits)
S2: 76 (33.7 bits)