IMP GPI Biosynthesis Report IMP-Bioinformatics
GPI Biosynthesis Main Page  
GPI Site Motif   GPI Site Prediction   Home Page B.E.  

GPI Anchor Biosynthesis Report: EMBL:AC087702 (GAA1 family, Trypanosoma brucei)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (461 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 4

Distribution of 12 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

emb|CAB86709.1| (AL163505) hypothetical protein L3640.14 [Leishm... 575 e-163
ref|NP_013189.1| (NC_001144) ER protein essential for attaching ... 346 6e-94
pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans 345 1e-93
ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans... 343 5e-93
emb|CAB65611.1| (AL136078) putative glycosylphosphatidylinositol... 340 3e-92
ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila mel... 316 4e-85
gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melano... 316 5e-85
ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mu... 290 4e-77
ref|NP_003792.1| (NM_003801) anchor attachment protein 1; anchor... 289 5e-77
pir||T46923 hypothetical protein DKFZp434E1512.1 - human >gi|701... 287 2e-76
ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis t... 272 9e-72
ref|NP_491701.1| (NM_059300) F33D11.9a.p [Caenorhabditis elegans... 109 8e-23
Alignments
>emb|CAB86709.1| (AL163505) hypothetical protein L3640.14 [Leishmania major]
          Length = 464

 Score =  575 bits (1467), Expect = e-163
 Identities = 168/455 (36%), Positives = 258/455 (55%), Gaps = 15/455 (3%)

Query: 11  REYGSTCAPLFLALGVCLICCLPAACPKENFIDENAISVSRVPVITGMLGELHMDTRISQ 70
           R +G   AP FL LG+ L+C LP    ++ +IDENA+     P         ++D  +  
Sbjct: 11  RHHGGKLAPFFLVLGIGLLCLLPVLRTRKIYIDENAVG-QMFPSADAGSVTAYLDPTVHW 69

Query: 71  YTRVVRGRRSVGSESIAVYVNAAFESSVVLANHLIYVLREKENMACDTNFYFFNDTSKDW 130
             R+VRGRRS GSE  AVYVN  + +SV LAN LI V+R ++++ACD  FYF N +S++W
Sbjct: 70  PQRLVRGRRSPGSEIAAVYVNTDYPASVSLANALIEVIRRRKSLACDVQFYFVN-SSEEW 128

Query: 131 PIPDSFVRAAIVINVTSFHTNNLCFNVFGANGMQPNQDLFNLAVAIAEKWRFNIDVLCQN 190
           P+P ++ RAA+V+N+++ +  ++C +  G NG+QPNQD  N+AV +A   +F    LCQ 
Sbjct: 129 PVPQAYTRAALVLNLSTLYNRHICIDTLGGNGIQPNQDYTNIAVQLAAANQFLPSYLCQR 188

Query: 191 PYISFSTSRPIYEHYFLALQTALVAPLRYQPWYKMRSRGISLLAISTEKSERRTKSSYGY 250
            + S   +   + HY+  L+ ++  P R QPW+ + +  I+ +AIS++     +      
Sbjct: 189 RHRSTDDAHAGFWHYWAYLEASVQLPTRPQPWHIIPTHSINTIAISSDPLYSASSVFPHS 248

Query: 251 NMAAAHEQL----IATLSYLHERFHHSTSVWIPLSVDQYVEYDVIQFATILFVASLLSTC 306
            +  A  QL    I +L+ L E+FHHST VW+P+S+ +YVEYD  QF  ILFVAS+ ST 
Sbjct: 249 QLPDAFAQLVLKIIVSLNGLEEKFHHSTFVWLPVSLWRYVEYDHAQFCIILFVASIFSTT 308

Query: 307 YSIYEVEGFSFSPCAALVSATGLVALVSTLNFGTAGFFV---SSGVLTVGLGAFAWDMSW 363
           YS Y++ G S +P   L     L  + +   F  AG++    +S   ++         + 
Sbjct: 309 YSEYQLRGISITP---LFVTLLLTPVAAAAAFQVAGWWAVVSASAAFSLLFRLLMGHANS 365

Query: 364 G---AINAVVLCLLIILQPVAGLVMGFGATLQLQFIHVRCRKWHVLLIGAILSWIVLIAL 420
           G   + NA+ LCLLIILQP  GLV G  A +Q+ FIH   +    + +GA +S  +    
Sbjct: 366 GMLLSFNAIALCLLIILQPACGLVAGAAAAIQVSFIHTALQNRPAMAVGAAVSSALAYYF 425

Query: 421 TEVMKAKLFDTETTAGVYFSFFLYPNALWISSRLI 455
              ++  LF  +  + ++ SF +YPNA+WI SR +
Sbjct: 426 IYHLRLPLFGVDGISELFVSFVIYPNAVWIGSRFL 460
>ref|NP_013189.1| (NC_001144) ER protein essential for attaching GPI
           (glycosylphosphatidylinositol) to protein; Gaa1p
           [Saccharomyces cerevisiae]
 sp|P39012|GAA1_YEAST GAA1 PROTEIN
 pir||S45053 membrane protein END2 - yeast (Saccharomyces cerevisiae)
 emb|CAA55944.1| (X79409) Gaa1 [Saccharomyces cerevisiae]
 gb|AAB67592.1| (U53880) Gaa1p [Saccharomyces cerevisiae]
 emb|CAA97649.1| (Z73260) ORF YLR088w [Saccharomyces cerevisiae]
          Length = 614

 Score =  346 bits (879), Expect = 6e-94
 Identities = 68/349 (19%), Positives = 126/349 (35%), Gaps = 43/349 (12%)

Query: 39  ENFIDENAISVSRVPVITGMLGELHMDTRISQYTRVVRGRRSVGSESIAVYVNAAFESSV 98
                E    V   P   G      M   +  +        ++G  ++ V +   F    
Sbjct: 113 NEQYGETLYGVMHAPRGDG---TEAMVLAVPWFN--SDDEFNIGGAALGVSLARFFSRWP 167

Query: 99  VLANHLIYVLREKENMACDTNFYFFNDTSKDWPIPDSFVRAAIVINVTSFHT--NNLCFN 156
           V + ++I V  E    A  +    ++ +     +    + AA+V++ +S       +  +
Sbjct: 168 VWSKNIIVVFSENPRAALRSWVEAYHTS---LDLTGGSIEAAVVLDYSSTEDFFEYVEIS 224

Query: 157 VFGANGMQPNQDLFNLAVAIAEKWRFN-------IDVLCQNPYISFSTSRPIYEHYFLAL 209
             G NG  PN DL N+A++I E             D L  N + S      +    +   
Sbjct: 225 YDGLNGELPNLDLVNIAISITEHEGMKVSLHGLPSDQLTNNNFWSRLKILCLGIRDWA-- 282

Query: 210 QTALVAPLRYQPWYKMRSRGISLLAISTEKSERRTKSSYGYNMAAAHEQLIATLSYLHER 269
            + +  P   + +   R + ++L A      +  T       +  A   +  +++ L E+
Sbjct: 283 LSGVKKPHGNEAFSGWRIQSVTLKAHGNSGHDITTFG----RIPEA---MFRSINNLLEK 335

Query: 270 FHHSTSVWIPLSVDQYVEYDVIQFATILFVASLLSTCYSIYEVEGFSFSPCA--ALVSAT 327
           FH S   ++ L+  Q+V       +     A  LS  ++I  +  F  +  A  +L S  
Sbjct: 336 FHQSFFFYLLLAPRQFVSISSYLPS-----AVALSIAFAISSLNAFINNAYANISLFSEY 390

Query: 328 GLVALVSTLNFGTAGFFVSSGVLTVGLGAFAWDMSWGAINAVVL--CLL 374
            LVAL+         +FVS  +  V   AF    S G +  + +  C L
Sbjct: 391 NLVALLV--------WFVSLVISFVVSQAFLLIPSSGLLMTISMASCFL 431
>pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans
          Length = 885

 Score =  345 bits (877), Expect = 1e-93
 Identities = 71/391 (18%), Positives = 118/391 (30%), Gaps = 79/391 (20%)

Query: 38  KENFIDENAISVSRVPVITGMLGELHMDTRISQYTRVVRGRRSVGSESIAVYVNAAFESS 97
                 EN     R P   G   E  M   + Q  R  + RR +      V         
Sbjct: 393 GNPMNGENVYGFIRGPRNDG--AEAQMI--VVQLGRSEKSRRMMSRMLAFVDYAKD---Q 445

Query: 98  VVLANHLIYVLR---------EKENMACDTNFYFFN-----DTSKDWPIPDSFVRA---- 139
           V  A  ++ V           EK   A D     +      ++ K   +    ++A    
Sbjct: 446 VYWARDIVIVFVDGGEKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGA 505

Query: 140 ---AIVINVTSFHTNN---LCFNVFGANGMQPNQDLFNLAVAIAEKWRFNIDVLCQNPYI 193
               +V +++         +     G NG Q N D+FN    IA       D    +   
Sbjct: 506 LIGGVVYDLSGMAVKGQHIVNIQTNGLNGQQVNLDVFNGITKIA-------DSKHHSRVA 558

Query: 194 SFSTSRPIYEHYFLALQTALVAPLRYQPWYKMRSRGISLLA-ISTEKSERRTKSSYGYNM 252
            F T       Y           + Y P+  +  R +   A IS E         YG   
Sbjct: 559 IFGTLHRHSNPY-----------MEYSPF-IVPMRALYTQAFISIEGIHSVL-GKYGVQG 605

Query: 253 AAA--------------HEQLIATLSYLHERFHHSTSVWIPLSVDQYVEYDVIQFATILF 298
                             E++   L+ + ER H S  +++      +    +      + 
Sbjct: 606 LTVGLSHDYSEKQTGQFIEEVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTIL 665

Query: 299 VASLLSTCYSIY-EVEGFSFSPCAALVSATG-LVALVSTLNFGTAGFFVSSGVLTVGLGA 356
           V+ L+ + Y  + +++ F F          G    + +T  F T G   ++ VL      
Sbjct: 666 VSPLVISAYFEWTKIQEFQFPVVHLAFHVLGFFYYIATTYVFQTIG---TTFVLQWMQWP 722

Query: 357 FAWD--------MSWGAINAVVLCLLIILQP 379
           F            SW  IN+ ++  +I L P
Sbjct: 723 FVGIDGCDGQFTPSWSLINSFLIFFMIPLGP 753
>ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans]
 gb|AAK68360.1|AF039720_13 (AF039720) Hypothetical protein F33D11.9b [Caenorhabditis elegans]
          Length = 670

 Score =  343 bits (871), Expect = 5e-93
 Identities = 71/391 (18%), Positives = 118/391 (30%), Gaps = 79/391 (20%)

Query: 38  KENFIDENAISVSRVPVITGMLGELHMDTRISQYTRVVRGRRSVGSESIAVYVNAAFESS 97
                 EN     R P   G   E  M   + Q  R  + RR +      V         
Sbjct: 131 GNPMNGENVYGFIRGPRNDG--AEAQMI--VVQLGRSEKSRRMMSRMLAFVDYAKD---Q 183

Query: 98  VVLANHLIYVLR---------EKENMACDTNFYFFN-----DTSKDWPIPDSFVRA---- 139
           V  A  ++ V           EK   A D     +      ++ K   +    ++A    
Sbjct: 184 VYWARDIVIVFVDGGEKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGA 243

Query: 140 ---AIVINVTSFHTNN---LCFNVFGANGMQPNQDLFNLAVAIAEKWRFNIDVLCQNPYI 193
               +V +++         +     G NG Q N D+FN    IA       D    +   
Sbjct: 244 LIGGVVYDLSGMAVKGQHIVNIQTNGLNGQQVNLDVFNGITKIA-------DSKHHSRVA 296

Query: 194 SFSTSRPIYEHYFLALQTALVAPLRYQPWYKMRSRGISLLA-ISTEKSERRTKSSYGYNM 252
            F T       Y           + Y P+  +  R +   A IS E         YG   
Sbjct: 297 IFGTLHRHSNPY-----------MEYSPF-IVPMRALYTQAFISIEGIHSVL-GKYGVQG 343

Query: 253 AAA--------------HEQLIATLSYLHERFHHSTSVWIPLSVDQYVEYDVIQFATILF 298
                             E++   L+ + ER H S  +++      +    +      + 
Sbjct: 344 LTVGLSHDYSEKQTGQFIEEVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTIL 403

Query: 299 VASLLSTCYSIY-EVEGFSFSPCAALVSATG-LVALVSTLNFGTAGFFVSSGVLTVGLGA 356
           V+ L+ + Y  + +++ F F          G    + +T  F T G   ++ VL      
Sbjct: 404 VSPLVISAYFEWTKIQEFQFPVVHLAFHVLGFFYYIATTYVFQTIG---TTFVLQWMQWP 460

Query: 357 FAWD--------MSWGAINAVVLCLLIILQP 379
           F            SW  IN+ ++  +I L P
Sbjct: 461 FVGIDGCDGQFTPSWSLINSFLIFFMIPLGP 491
>emb|CAB65611.1| (AL136078) putative glycosylphosphatidylinositol anchor attachment
           protein [Schizosaccharomyces pombe]
          Length = 581

 Score =  340 bits (864), Expect = 3e-92
 Identities = 54/339 (15%), Positives = 111/339 (31%), Gaps = 35/339 (10%)

Query: 37  PKENFIDENAISVSRVPVITGMLGELHMDTRISQYTRVVRGRRSVGSESIAVYVNAAFES 96
           P   F   N I+  R P          +   +     +  G+ +    ++A+ +   F+ 
Sbjct: 117 PGSEFNGSNFITTLRAPRGDA---TESLLLCVPWKDHI--GQYNEAGVALAISLLKYFQG 171

Query: 97  SVVLANHLIYVLREKENMACDTNFYFFND------TSKDWPIPDSFVRAAIVINVTSFHT 150
             + +  +I V+ +       +    + D      +     I    ++A + + + +   
Sbjct: 172 WSLWSKDIILVIFDDPVYGPSSFLTSYFDQTTPYISYTPLKIRSGSIQAGLSLELVTTEN 231

Query: 151 NNLCFNVF--GANGMQPNQDLFNLAVAIAEKWRFNI----DVLCQNPYISFSTSRPIYEH 204
           N+    V     NG  PN DLFN    I  +                  S  TSR   + 
Sbjct: 232 NSDVLEVLYQATNGQLPNLDLFNTISRIFMQHFNYPLRLQGYDFHANSGSSYTSR--LKS 289

Query: 205 YFLALQTALVA--PLRYQPWYKMRSRGISLLAISTEKSERRTKSSYGYNMAAAHEQLIAT 262
            ++ + T  V+     +  + + R   ++L     +       S   +    A E    +
Sbjct: 290 LWMGMLTQAVSNVTSAHALFPQYRIDMLTLRMKVKDP-----FSFDMFRFGQAIESTFRS 344

Query: 263 LSYLHERFHHSTSVWIPLSVDQYVEYDVIQFATILFVASLLSTCYSIY-----EV---EG 314
           L+ L E  H S   +  L    ++       + ++  AS +   Y  +     ++     
Sbjct: 345 LNNLLEHLHQSFFFYFILDHLHFISIGNYMPSILILAASFMLGAYRHWINHEKKIDLWRP 404

Query: 315 FSFSPCAALVSATGLVALVSTLNFGTAGFFVSSGVLTVG 353
           FSF    ++        LVS+    T   F+   +  +G
Sbjct: 405 FSFWLF-SIFCTIAAYYLVSSSTKITVFIFLYLMLTFIG 442
>ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila melanogaster]
 gb|AAK77275.1| (AY047543) GH05723p [Drosophila melanogaster]
          Length = 561

 Score =  316 bits (803), Expect = 4e-85
 Identities = 54/295 (18%), Positives = 103/295 (34%), Gaps = 26/295 (8%)

Query: 38  KENFIDENAISVSRVPVITGMLGELHMDTRISQYTRVVRGRRSVGSESIAVYVNAAFESS 97
            + +  +N   + R P I        +                  S  + +         
Sbjct: 122 GKEYHGKNIYGILRAPRI---ASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRK 178

Query: 98  VVLANHLIYVLREKENMACDTNFYFFNDTSKDWPI------------PDSFVRAAIVINV 145
              A  LI+++ E+E +        ++D  ++  +                ++AA+ I V
Sbjct: 179 NYWAKDLIFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAALNIEV 238

Query: 146 TSFHTNNLCFNVFGANGMQPNQDLFNLAVAIAEKWRFNIDVLCQNPYISFSTSRPIYEHY 205
                +++   + G NG  PN D+FNL   I  +         Q P      S+  +E  
Sbjct: 239 QDLEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYK-QAPRKKRRHSQSHFEQN 297

Query: 206 FLALQTALVAPLRYQP------WYKMRSRGISLLAISTEKSERRTKSSYGYN---MAAAH 256
           F  + T L +     P      +++ R   ++ +A +   +    K S G     +  A 
Sbjct: 298 FRQMLTMLASQSSGVPTGNHGLFHRYRIDALT-IAANRRATHATLKGSPGSAAVPLLKAI 356

Query: 257 EQLIATLSYLHERFHHSTSVWIPLSVDQYVEYDVIQFATILFVASLLSTCYSIYE 311
           E +  +L+ L ERFH S   ++ +S D+Y+       A +  VA      Y  + 
Sbjct: 357 EGIARSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWS 411
>gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melanogaster]
          Length = 674

 Score =  316 bits (803), Expect = 5e-85
 Identities = 54/295 (18%), Positives = 103/295 (34%), Gaps = 26/295 (8%)

Query: 38  KENFIDENAISVSRVPVITGMLGELHMDTRISQYTRVVRGRRSVGSESIAVYVNAAFESS 97
            + +  +N   + R P I        +                  S  + +         
Sbjct: 122 GKEYHGKNIYGILRAPRI---ASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRK 178

Query: 98  VVLANHLIYVLREKENMACDTNFYFFNDTSKDWPI------------PDSFVRAAIVINV 145
              A  LI+++ E+E +        ++D  ++  +                ++AA+ I V
Sbjct: 179 NYWAKDLIFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAALNIEV 238

Query: 146 TSFHTNNLCFNVFGANGMQPNQDLFNLAVAIAEKWRFNIDVLCQNPYISFSTSRPIYEHY 205
                +++   + G NG  PN D+FNL   I  +         Q P      S+  +E  
Sbjct: 239 QDLEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYK-QAPRKKRRHSQSHFEQN 297

Query: 206 FLALQTALVAPLRYQP------WYKMRSRGISLLAISTEKSERRTKSSYGYN---MAAAH 256
           F  + T L +     P      +++ R   ++ +A +   +    K S G     +  A 
Sbjct: 298 FRQMLTMLASQSSGVPTGNHGLFHRYRIDALT-IAANRRATHATLKGSPGSAAVPLLKAI 356

Query: 257 EQLIATLSYLHERFHHSTSVWIPLSVDQYVEYDVIQFATILFVASLLSTCYSIYE 311
           E +  +L+ L ERFH S   ++ +S D+Y+       A +  VA      Y  + 
Sbjct: 357 EGIARSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWS 411
>ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mus musculus]
 dbj|BAA82589.1| (AB002136) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAA82591.1| (AB002138) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAB03274.1| (AB006970) GPI anchor attachment protein [Mus musculus]
 dbj|BAB03275.1| (AB006971) GPI anchor attachment protein [Mus musculus]
 gb|AAH06697.1|AAH06697 (BC006697) GPI anchor attachment protein 1 [Mus musculus]
          Length = 621

 Score =  290 bits (735), Expect = 4e-77
 Identities = 43/266 (16%), Positives = 94/266 (35%), Gaps = 26/266 (9%)

Query: 43  DENAISVSRVPVITGMLGELHMDTRISQYTRVVRGRRSVGSESIAVYVNAAFESSVVLAN 102
             N   + R P          +   +         +    +  + + + A F   +  A 
Sbjct: 126 GTNVYGILRAPR---SASTESLVLTVPCGPDATNSQ----AVGLLLALAAHFRGQIYWAK 178

Query: 103 HLIYVLREKENMACDTNFYFFND------TSKDWPIPDSFVRAAIVINVTSFHTNNLCFN 156
            +I+++ + + +  +     ++D       S         ++AA+ + ++S    +L   
Sbjct: 179 DIIFLVTDHDLLGTEAWLEAYHDINVTGIQSSPLQGRAGAIQAAVALELSSDVVTSLDVT 238

Query: 157 VFGANGMQPNQDLFNLAVAIAEKWRFNIDVLCQNPYISFS-TSRPIYEHYFLALQTALVA 215
           V G NG  PN DL NL     +K      +  Q        TS          L   ++ 
Sbjct: 239 VEGLNGQLPNLDLLNLFQTFCQKGGLLCTL--QGKLQPQDWTSLEGPLQGLQTLLLMVLR 296

Query: 216 PLRYQP------WYKMRSRGISLLAISTEKSERRTKSSYGYNMAAAHEQLIATLSYLHER 269
               +P      + +     ++L  I++ +  +   ++ G     A E +   L++L ER
Sbjct: 297 QASGRPHGPHGLFLRYGVEALTLRGINSFRQYKYDLATVG----KALEGMFRKLNHLLER 352

Query: 270 FHHSTSVWIPLSVDQYVEYDVIQFAT 295
            H S   ++  ++ ++V   +   AT
Sbjct: 353 LHQSFFFYLLPALSRFVSIGLYMPAT 378
>ref|NP_003792.1| (NM_003801) anchor attachment protein 1; anchor attachment protein
           1 (Gaa1p, yeast) homolog; glycophosphatidylinositol
           anchor attachment 1 [Homo sapiens]
 dbj|BAA24035.1| (AB006969) hGAA1 [Homo sapiens]
 dbj|BAA82588.1| (AB002135) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Homo sapiens]
 dbj|BAA82590.1| (AB002137) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Homo sapiens]
 gb|AAH03171.1|AAH03171 (BC003171) anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo
           sapiens]
 gb|AAH04129.1|AAH04129 (BC004129) anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo
           sapiens]
          Length = 621

 Score =  289 bits (734), Expect = 5e-77
 Identities = 43/265 (16%), Positives = 94/265 (35%), Gaps = 26/265 (9%)

Query: 43  DENAISVSRVPVITGMLGELHMDTRISQYTRVVRGRRSVGSESIAVYVNAAFESSVVLAN 102
             N   + R P          +   +   +     +    +  + + + A F   +  A 
Sbjct: 126 GTNVYGILRAPRA---ASTESLVLTVPCGSDSTNSQ----AVGLLLALAAHFRGQIYWAK 178

Query: 103 HLIYVLREKENMACDTNFYFFND------TSKDWPIPDSFVRAAIVINVTSFHTNNLCFN 156
            +++++ E + +  +     ++D       S         ++AA+ + ++S    +L   
Sbjct: 179 DIVFLVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVA 238

Query: 157 VFGANGMQPNQDLFNLAVAIAEKWRFNIDVLCQNPYISFS-TSRPIYEHYFLALQTALVA 215
           V G NG  PN DL NL     +K      +  Q        TS          L   ++ 
Sbjct: 239 VEGLNGQLPNLDLLNLFQTFCQKGGLLCTL--QGKLQPEDWTSLDGPLQGLQTLLLMVLR 296

Query: 216 PLRYQP------WYKMRSRGISLLAISTEKSERRTKSSYGYNMAAAHEQLIATLSYLHER 269
               +P      + + R   ++L  I++ +  +    + G     A E +   L++L ER
Sbjct: 297 QASGRPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVG----KALEGMFRKLNHLLER 352

Query: 270 FHHSTSVWIPLSVDQYVEYDVIQFA 294
            H S  +++   + ++V   +   A
Sbjct: 353 LHQSFFLYLLPGLSRFVSIGLYMPA 377
>pir||T46923 hypothetical protein DKFZp434E1512.1 - human
 emb|CAB75660.1| (AL157437) hypothetical protein [Homo sapiens]
 gb|AAH06383.1|AAH06383 (BC006383) Unknown (protein for MGC:12663) [Homo sapiens]
          Length = 499

 Score =  287 bits (729), Expect = 2e-76
 Identities = 43/265 (16%), Positives = 94/265 (35%), Gaps = 26/265 (9%)

Query: 43  DENAISVSRVPVITGMLGELHMDTRISQYTRVVRGRRSVGSESIAVYVNAAFESSVVLAN 102
             N   + R P          +   +   +     +    +  + + + A F   +  A 
Sbjct: 4   GTNVYGILRAPRA---ASTESLVLTVPCGSDSTNSQ----AVGLLLALAAHFRGQIYWAK 56

Query: 103 HLIYVLREKENMACDTNFYFFND------TSKDWPIPDSFVRAAIVINVTSFHTNNLCFN 156
            +++++ E + +  +     ++D       S         ++AA+ + ++S    +L   
Sbjct: 57  DIVFLVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVA 116

Query: 157 VFGANGMQPNQDLFNLAVAIAEKWRFNIDVLCQNPYISFS-TSRPIYEHYFLALQTALVA 215
           V G NG  PN DL NL     +K      +  Q        TS          L   ++ 
Sbjct: 117 VEGLNGQLPNLDLLNLFQTFCQKGGLLCTL--QGKLQPEDWTSLDGPLQGLQTLLLMVLR 174

Query: 216 PLRYQP------WYKMRSRGISLLAISTEKSERRTKSSYGYNMAAAHEQLIATLSYLHER 269
               +P      + + R   ++L  I++ +  +    + G     A E +   L++L ER
Sbjct: 175 QASGRPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVG----KALEGMFRKLNHLLER 230

Query: 270 FHHSTSVWIPLSVDQYVEYDVIQFA 294
            H S  +++   + ++V   +   A
Sbjct: 231 LHQSFFLYLLPGLSRFVSIGLYMPA 255
>ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis thaliana]
          Length = 696

 Score =  272 bits (690), Expect = 9e-72
 Identities = 53/325 (16%), Positives = 102/325 (31%), Gaps = 64/325 (19%)

Query: 43  DENAISVSRVPVITGMLGELHMDTRISQYTRVVRGRRSVGSESIAVYVNAAFESSVVLAN 102
             N   + R P   G   +  +      Y  +  G     S  I   + +       L+ 
Sbjct: 155 GVNVAGIIRAPRGDG---KESIVLVTP-YDFINGGDYEDLSLGIVSSLFSLLSRVTWLSK 210

Query: 103 HLIYVLRE------KENMACDTNFYFFND-TSKDWPI-------------PDSFVRAAIV 142
            +I+++ +      +   A       ++  + K   +                 + AA+V
Sbjct: 211 DIIWLVADSRYGDYRPVAA---WLTEYHSPSFKVSDLLKCDEQNTADNFRRAGTMAAALV 267

Query: 143 INV---TSFHTNNLCFNVFGANGMQPNQDLFNLA-------------------------V 174
           + V   +    + L      +NG  PN DL N+                          +
Sbjct: 268 LKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVNYLAVHRQGFYVKVEKVVSLLSSSWL 327

Query: 175 AIAEKWRFNIDVLCQ--NPYISFSTSRPIYEHYFLALQTALVAPLRYQP------WYKMR 226
            I  +    +  L    NP  +F      Y      L ++L +     P      +   +
Sbjct: 328 KIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEGSATLASSLYSQALGIPTGPHGAFRDYQ 387

Query: 227 SRGISLLAISTEKSERRTKSSYGY-NMAAAHEQLIATLSYLHERFHHSTSVWIPLSVDQY 285
              I+L        + +T+    +   A   E  I +++ L E+FH S  +++  S  ++
Sbjct: 388 VDAITLKVSPRFPPDSKTRQHDFFLRGARLLEGTIRSVNNLLEKFHQSFFLYMLTSPSKF 447

Query: 286 VEYDVIQFATILFVASLLSTCYSIY 310
           +   V   A  L VA L     S+Y
Sbjct: 448 ISVGVYMIAFALLVAPLPMVAASLY 472
>ref|NP_491701.1| (NM_059300) F33D11.9a.p [Caenorhabditis elegans]
 pir||T32772 hypothetical protein F33D11.9a - Caenorhabditis elegans
 gb|AAB96702.1| (AF039720) Hypothetical protein F33D11.9a [Caenorhabditis elegans]
          Length = 229

 Score =  109 bits (272), Expect = 8e-23
 Identities = 20/100 (20%), Positives = 37/100 (37%), Gaps = 13/100 (13%)

Query: 290 VIQFATILFVASLLSTCYSIY-EVEGFSFSPCAALVSATG-LVALVSTLNFGTAGFFVSS 347
           +      + V+ L+ + Y  + +++ F F          G    + +T  F T G   ++
Sbjct: 1   MYIIPLTILVSPLVISAYFEWTKIQEFQFPVVHLAFHVLGFFYYIATTYVFQTIG---TT 57

Query: 348 GVLTVGLGAFAWD--------MSWGAINAVVLCLLIILQP 379
            VL      F            SW  IN+ ++  +I L P
Sbjct: 58  FVLQWMQWPFVGIDGCDGQFTPSWSLINSFLIFFMIPLGP 97
CPU time:   115.27 user secs.	    2.45 sys. secs	  117.72 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.319    0.171    0.495 

Gapped
Lambda     K      H
   0.270   0.0601    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 276200379
Number of Sequences: 887402
Number of extensions: 12774785
Number of successful extensions: 50041
Number of sequences better than 10.0: 327
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 328
Number of HSP's that attempted gapping in prelim test: 49653
Number of HSP's gapped (non-prelim): 518
length of query: 461
length of database: 277,845,442
effective HSP length: 54
effective length of query: 407
effective length of database: 229,925,734
effective search space: 93579773738
effective search space used: 93579773738
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.9 bits)
S2: 75 (33.3 bits)