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GPI Anchor Biosynthesis Report: CAB65611.1 (GAA1 family, Schizosaccharomyces pombe)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (581 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 2

Distribution of 12 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

emb|CAB65611.1| (AL136078) putative glycosylphosphatidylinositol... 793 0.0
ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans... 672 0.0
pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans 649 0.0
ref|NP_013189.1| (NC_001144) ER protein essential for attaching ... 560 e-158
ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis t... 509 e-143
ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila mel... 486 e-136
gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melano... 483 e-135
ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mu... 477 e-133
ref|NP_003792.1| (NM_003801) anchor attachment protein 1; anchor... 466 e-130
pir||T46923 hypothetical protein DKFZp434E1512.1 - human >gi|701... 371 e-101
ref|NP_491701.1| (NM_059300) F33D11.9a.p [Caenorhabditis elegans... 211 4e-53
Sequences with E-value WORSE than threshold

emb|CAB86709.1| (AL163505) hypothetical protein L3640.14 [Leishm... 41 0.051
Alignments
>emb|CAB65611.1| (AL136078) putative glycosylphosphatidylinositol anchor attachment
           protein [Schizosaccharomyces pombe]
          Length = 581

 Score =  793 bits (2028), Expect = 0.0
 Identities = 581/581 (100%), Positives = 581/581 (100%)

Query: 1   MSLFTFVQIRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNT 60
           MSLFTFVQIRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNT
Sbjct: 1   MSLFTFVQIRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNT 60

Query: 61  YFENRYSKTVSSSLTAANTWSHLDASVGTNTMYDDLEQIFTAMGLPTQKQNYSINIPGSE 120
           YFENRYSKTVSSSLTAANTWSHLDASVGTNTMYDDLEQIFTAMGLPTQKQNYSINIPGSE
Sbjct: 61  YFENRYSKTVSSSLTAANTWSHLDASVGTNTMYDDLEQIFTAMGLPTQKQNYSINIPGSE 120

Query: 121 FNGSNFITTLRAPRGDATESLLLCVPWKDHIGQYNEAGVALAISLLKYFQGWSLWSKDII 180
           FNGSNFITTLRAPRGDATESLLLCVPWKDHIGQYNEAGVALAISLLKYFQGWSLWSKDII
Sbjct: 121 FNGSNFITTLRAPRGDATESLLLCVPWKDHIGQYNEAGVALAISLLKYFQGWSLWSKDII 180

Query: 181 LVIFDDPVYGPSSFLTSYFDQTTPYISYTPLKIRSGSIQAGLSLELVTTENNSDVLEVLY 240
           LVIFDDPVYGPSSFLTSYFDQTTPYISYTPLKIRSGSIQAGLSLELVTTENNSDVLEVLY
Sbjct: 181 LVIFDDPVYGPSSFLTSYFDQTTPYISYTPLKIRSGSIQAGLSLELVTTENNSDVLEVLY 240

Query: 241 QATNGQLPNLDLFNTISRIFMQHFNYPLRLQGYDFHANSGSSYTSRLKSLWMGMLTQAVS 300
           QATNGQLPNLDLFNTISRIFMQHFNYPLRLQGYDFHANSGSSYTSRLKSLWMGMLTQAVS
Sbjct: 241 QATNGQLPNLDLFNTISRIFMQHFNYPLRLQGYDFHANSGSSYTSRLKSLWMGMLTQAVS 300

Query: 301 NVTSAHALFPQYRIDMLTLRMKVKDPFSFDMFRFGQAIESTFRSLNNLLEHLHQSFFFYF 360
           NVTSAHALFPQYRIDMLTLRMKVKDPFSFDMFRFGQAIESTFRSLNNLLEHLHQSFFFYF
Sbjct: 301 NVTSAHALFPQYRIDMLTLRMKVKDPFSFDMFRFGQAIESTFRSLNNLLEHLHQSFFFYF 360

Query: 361 ILDHLHFISIGNYMPSILILAASFMLGAYRHWINHEKKIDLWRPFSFWLFSIFCTIAAYY 420
           ILDHLHFISIGNYMPSILILAASFMLGAYRHWINHEKKIDLWRPFSFWLFSIFCTIAAYY
Sbjct: 361 ILDHLHFISIGNYMPSILILAASFMLGAYRHWINHEKKIDLWRPFSFWLFSIFCTIAAYY 420

Query: 421 LVSSSTKITVFIFLYLMLTFIGIIFSTFMTSEDAELVLSYDLMSKSLFISVVSTLNFSLS 480
           LVSSSTKITVFIFLYLMLTFIGIIFSTFMTSEDAELVLSYDLMSKSLFISVVSTLNFSLS
Sbjct: 421 LVSSSTKITVFIFLYLMLTFIGIIFSTFMTSEDAELVLSYDLMSKSLFISVVSTLNFSLS 480

Query: 481 FVVAILLVPLQFISFRFNRRLSLLFAVLTYFSTFIFLCSLSKILNGPLVPFWLWAKEYEL 540
           FVVAILLVPLQFISFRFNRRLSLLFAVLTYFSTFIFLCSLSKILNGPLVPFWLWAKEYEL
Sbjct: 481 FVVAILLVPLQFISFRFNRRLSLLFAVLTYFSTFIFLCSLSKILNGPLVPFWLWAKEYEL 540

Query: 541 FNSWLMPSVFMILVLPEIIFSVTSFFSLWNEPSVKTKTKTL 581
           FNSWLMPSVFMILVLPEIIFSVTSFFSLWNEPSVKTKTKTL
Sbjct: 541 FNSWLMPSVFMILVLPEIIFSVTSFFSLWNEPSVKTKTKTL 581
>ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans]
 gb|AAK68360.1|AF039720_13 (AF039720) Hypothetical protein F33D11.9b [Caenorhabditis elegans]
          Length = 670

 Score =  672 bits (1717), Expect = 0.0
 Identities = 129/601 (21%), Positives = 240/601 (39%), Gaps = 89/601 (14%)

Query: 22  LQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFENRYSKTVSSSLTAANTWS 81
           +      +   W+F  P N  + R  +SE +L+PG V   F+         S  A   + 
Sbjct: 46  IAYIGAYLSAQWLFD-PVNMEVTR--ISEHSLMPGLVTPKFDK--------SGIAIQLYR 94

Query: 82  HLDASVGTNTMYDDLEQIFTAMGLPTQKQNYSINIPGSEFNGSNFITTLRAPRGDATESL 141
                  + +  + +  IF+ +GL      +   + G+  NG N    +R PR D  E+ 
Sbjct: 95  RFSDLPKSKSQQELIHTIFSDLGLECFTHKWRSKVAGNPMNGENVYGFIRGPRNDGAEAQ 154

Query: 142 LLCVPWKDHIGQYNEAGVALA--ISLLKYFQGWSLWSKDIILVIFD---------DPVYG 190
           ++ V     +G+  ++   ++  ++ + Y +    W++DI++V  D            + 
Sbjct: 155 MIVV----QLGRSEKSRRMMSRMLAFVDYAKDQVYWARDIVIVFVDGGEKNDSIEKAAFA 210

Query: 191 PSSFLTSY------FDQTTPYISYTPLKIRSGSIQAGLSLELVTT-ENNSDVLEVLYQAT 243
             +FL  Y        + +  +    ++ ++G++  G+  +L         ++ +     
Sbjct: 211 LDAFLLKYQKIEALNSKKSITVEADEIQAQTGALIGGVVYDLSGMAVKGQHIVNIQTNGL 270

Query: 244 NGQLPNLDLFNTISRIF-MQHFNYPLRLQGYDFHANSGSSYTSRLKSLWMGMLTQAVSNV 302
           NGQ  NLD+FN I++I   +H +          H+N    Y+  +  +   + TQA  ++
Sbjct: 271 NGQQVNLDVFNGITKIADSKHHSRVAIFGTLHRHSNPYMEYSPFIVPM-RALYTQAFISI 329

Query: 303 TSAHALFPQYRIDMLTLRMKVKDPFSFDMFRFGQAIESTFRSLNNLLEHLHQSFFFYFIL 362
              H++  +Y +  LT+ +       +   + GQ IE   R LNN+LE LHQS+F Y + 
Sbjct: 330 EGIHSVLGKYGVQGLTVGLS----HDYSEKQTGQFIEEVSRMLNNVLERLHQSYFMYVLA 385

Query: 363 DHLHFISIGNYMPSILILAASFMLGAYRHWINHEKKIDLWRPFSFWLFSIFCTIAAYYLV 422
           D LHF SI  Y+  + IL +  ++ AY  W   ++        +F +   F  IA  Y+ 
Sbjct: 386 DDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQEFQFPVVHLAFHVLGFFYYIATTYVF 445

Query: 423 SSSTKITVFIFLYLMLTFIGIIFSTFMTSEDAELVLSYDLMSKSLFI------------- 469
              T  T F+  ++   F+GI         D +   S+ L++  L               
Sbjct: 446 --QTIGTTFVLQWMQWPFVGI------DGCDGQFTPSWSLINSFLIFFMIPLGPYVFLKE 497

Query: 470 -----------SVVST---------LNFSLSFVVAILLVPLQFISFRFNRRLSLLFAVLT 509
                       ++ T          NF L+ +    +VP+ F+    +   S + A+L 
Sbjct: 498 PVRCVPSMRIVCLLETSLVLYGICLTNFGLAILCTAFVVPIVFLMTTRSIFRSSVLAILN 557

Query: 510 YFSTFIFLCSLSKILNGPLVPFWLWAKEYELFNSWLMP--SVFMILVLPEIIFSVTSFFS 567
             S   F+      + G L P           +SWL    S+ +  +  ++ F V  FF 
Sbjct: 558 PISVLAFVAINIPGMFGQLFPTISQ-------DSWLAAAQSIALTAIREDVEFKVNHFFL 610

Query: 568 L 568
           L
Sbjct: 611 L 611
>pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans
          Length = 885

 Score =  649 bits (1657), Expect = 0.0
 Identities = 125/568 (22%), Positives = 232/568 (40%), Gaps = 70/568 (12%)

Query: 22  LQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFENRYSKTVSSSLTAANTWS 81
           +      +   W+F  P N  + R  +SE +L+PG V   F+         S  A   + 
Sbjct: 308 IAYIGAYLSAQWLFD-PVNMEVTR--ISEHSLMPGLVTPKFDK--------SGIAIQLYR 356

Query: 82  HLDASVGTNTMYDDLEQIFTAMGLPTQKQNYSINIPGSEFNGSNFITTLRAPRGDATESL 141
                  + +  + +  IF+ +GL      +   + G+  NG N    +R PR D  E+ 
Sbjct: 357 RFSDLPKSKSQQELIHTIFSDLGLECFTHKWRSKVAGNPMNGENVYGFIRGPRNDGAEAQ 416

Query: 142 LLCVPWKDHIGQYNEAGVALA--ISLLKYFQGWSLWSKDIILVIFD---------DPVYG 190
           ++ V     +G+  ++   ++  ++ + Y +    W++DI++V  D            + 
Sbjct: 417 MIVV----QLGRSEKSRRMMSRMLAFVDYAKDQVYWARDIVIVFVDGGEKNDSIEKAAFA 472

Query: 191 PSSFLTSY------FDQTTPYISYTPLKIRSGSIQAGLSLELVTT-ENNSDVLEVLYQAT 243
             +FL  Y        + +  +    ++ ++G++  G+  +L         ++ +     
Sbjct: 473 LDAFLLKYQKIEALNSKKSITVEADEIQAQTGALIGGVVYDLSGMAVKGQHIVNIQTNGL 532

Query: 244 NGQLPNLDLFNTISRIF-MQHFNYPLRLQGYDFHANSGSSYTSRLKSLWMGMLTQAVSNV 302
           NGQ  NLD+FN I++I   +H +          H+N    Y+  +  +   + TQA  ++
Sbjct: 533 NGQQVNLDVFNGITKIADSKHHSRVAIFGTLHRHSNPYMEYSPFIVPM-RALYTQAFISI 591

Query: 303 TSAHALFPQYRIDMLTLRMKVKDPFSFDMFRFGQAIESTFRSLNNLLEHLHQSFFFYFIL 362
              H++  +Y +  LT+ +       +   + GQ IE   R LNN+LE LHQS+F Y + 
Sbjct: 592 EGIHSVLGKYGVQGLTVGLS----HDYSEKQTGQFIEEVSRMLNNVLERLHQSYFMYVLA 647

Query: 363 DHLHFISIGNYMPSILILAASFMLGAYRHWINHEKKIDLWRPFSFWLFSIFCTIAAYYLV 422
           D LHF SI  Y+  + IL +  ++ AY  W   ++        +F +   F  IA  Y+ 
Sbjct: 648 DDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQEFQFPVVHLAFHVLGFFYYIATTYVF 707

Query: 423 SSSTKITVFIFLYLMLTFIGIIFSTFMTSEDAELVLSYDLMSKSLFISVVSTLNFSLSFV 482
              T  T F+  ++   F+GI         D +   S+ L++  L   ++          
Sbjct: 708 --QTIGTTFVLQWMQWPFVGI------DGCDGQFTPSWSLINSFLIFFMIP--------- 750

Query: 483 VAILLVPLQFISFRFNRRLSLLFAVLTYFSTFIFLCSLSKILNGPLVPFWLWAKEYELFN 542
               L P  F+    +   S + A+L   S   F+      + G L P           +
Sbjct: 751 ----LGPYVFLK-TRSIFRSSVLAILNPISVLAFVAINIPGMFGQLFPTISQ-------D 798

Query: 543 SWLMP--SVFMILVLPEIIFSVTSFFSL 568
           SWL    S+ +  +  ++ F V  FF L
Sbjct: 799 SWLAAAQSIALTAIREDVEFKVNHFFLL 826
>ref|NP_013189.1| (NC_001144) ER protein essential for attaching GPI
           (glycosylphosphatidylinositol) to protein; Gaa1p
           [Saccharomyces cerevisiae]
 sp|P39012|GAA1_YEAST GAA1 PROTEIN
 pir||S45053 membrane protein END2 - yeast (Saccharomyces cerevisiae)
 emb|CAA55944.1| (X79409) Gaa1 [Saccharomyces cerevisiae]
 gb|AAB67592.1| (U53880) Gaa1p [Saccharomyces cerevisiae]
 emb|CAA97649.1| (Z73260) ORF YLR088w [Saccharomyces cerevisiae]
          Length = 614

 Score =  560 bits (1430), Expect = e-158
 Identities = 148/505 (29%), Positives = 248/505 (48%), Gaps = 45/505 (8%)

Query: 7   VQIRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFENRY 66
           V + + P +   L  + +   L G   I ILP +    R ++SE+AL+P Q  +YF    
Sbjct: 12  VDMGLVPRIIALLPVISMLCALFGFISIAILPMDGQYRRTYISENALMPSQAYSYFRESE 71

Query: 67  SKTVSSSLTAANTWSHLDASVGTNTMYDDLEQIFTAMGLPTQKQNYSINIPGSEFNGSNF 126
              +    +      ++ +    N M   L++  T   +   +Q            G   
Sbjct: 72  WNILRGYRSQIKEMVNMTSMERNNLMGSWLQEFGTKTAIYENEQ-----------YGETL 120

Query: 127 ITTLRAPRGDATESLLLCVPWKDHIGQYNEAGVALAISLLKYFQGWSLWSKDIILVIFDD 186
              + APRGD TE+++L VPW +   ++N  G AL +SL ++F  W +WSK+II+V  ++
Sbjct: 121 YGVMHAPRGDGTEAMVLAVPWFNSDDEFNIGGAALGVSLARFFSRWPVWSKNIIVVFSEN 180

Query: 187 PVYGPSSFLTSYFDQTTPYISYTPLKIRSGSIQAGLSLELVTTENNSDVLEVLYQATNGQ 246
           P     S++ +Y          T L +  GSI+A + L+  +TE+  + +E+ Y   NG+
Sbjct: 181 PRAALRSWVEAYH---------TSLDLTGGSIEAAVVLDYSSTEDFFEYVEISYDGLNGE 231

Query: 247 LPNLDLFNTISRIFMQHFNYPLRLQGYDFHANSGSSYTSRLKSLWMGMLTQAVSNVTSAH 306
           LPNLDL N I+    +H    + L G      + +++ SRLK L +G+   A+S V   H
Sbjct: 232 LPNLDLVN-IAISITEHEGMKVSLHGLPSDQLTNNNFWSRLKILCLGIRDWALSGVKKPH 290

Query: 307 A--LFPQYRIDMLTLRMKVKDPFSFDMFRFGQAIESTFRSLNNLLEHLHQSFFFYFILDH 364
               F  +RI  +TL  K       D+  FG+  E+ FRS+NNLLE  HQSFFFY +L  
Sbjct: 291 GNEAFSGWRIQSVTL--KAHGNSGHDITTFGRIPEAMFRSINNLLEKFHQSFFFYLLLAP 348

Query: 365 LHFISIGNYMPSILILAASFMLGAYRHWINHE-KKIDLWRPFSF-----WLFSI---FCT 415
             F+SI +Y+PS + L+ +F + +   +IN+    I L+  ++      W  S+   F  
Sbjct: 349 RQFVSISSYLPSAVALSIAFAISSLNAFINNAYANISLFSEYNLVALLVWFVSLVISFVV 408

Query: 416 IAAYYLVSSSTKITVFIFLYLMLTFIGIIFSTFMTSEDAELVLSYDLMS-KSLFISVVST 474
             A+ L+ SS    + + + +   F+ +I S  +   +    LSY L +   L+ S+VST
Sbjct: 409 SQAFLLIPSS---GLLMTISMASCFLPLILSRKIHISEP---LSYRLKNVAFLYFSLVST 462

Query: 475 ----LNFSLSFVVAILLVPLQFISF 495
               +NF+++ ++  L  P+ F+  
Sbjct: 463 SLLMINFAMALLIGTLAFPMTFVKT 487
>ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis thaliana]
          Length = 696

 Score =  509 bits (1297), Expect = e-143
 Identities = 137/623 (21%), Positives = 246/623 (38%), Gaps = 119/623 (19%)

Query: 10  RVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFENRYSKT 69
           R+  FL  H     +  +  G+  + +LP        ++SE+AL+PG   +   NR    
Sbjct: 28  RLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKN--TYISENALMPGSARSMLSNRDVSD 85

Query: 70  VSSSLTAANTWSHLDASVGTNTMYDDLEQIFTAMGLPTQKQNYSIN---------IPGSE 120
            S  +     +           +   + +  + MG     Q +              G +
Sbjct: 86  GSKLVKDIKNFR----LNHEGQVQKLIGKYMSDMGAEVSYQKFHPEGNQFHPLHFFSGPD 141

Query: 121 -----------FNGSNFITTLRAPRGDATESLLLCVPW-KDHIGQYNEAGVALAISLLKY 168
                        G N    +RAPRGD  ES++L  P+   + G Y +  + +  SL   
Sbjct: 142 SYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGIVSSLFSL 201

Query: 169 FQGWSLWSKDIILVIFDDPV--YGP-SSFLTSYFDQTTPYISYTPLKI-----------R 214
               +  SKDII ++ D     Y P +++LT Y    +P    + L             R
Sbjct: 202 LSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYH---SPSFKVSDLLKCDEQNTADNFRR 258

Query: 215 SGSIQAGLSLEL-VTTENNSDVLEVLYQATNGQLPNLDLFNTISRIFMQHFNYPLRLQ-- 271
           +G++ A L L++   +E   D L +  +A+NGQ+PNLDL N ++ + +    + ++++  
Sbjct: 259 AGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVNYLAVHRQGFYVKVEKV 318

Query: 272 -------------------GYDFHAN--------SGSSYTSRLKSLWMGMLTQAVSNVTS 304
                              G   H            + Y     +L   + +QA+   T 
Sbjct: 319 VSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYLEGSATLASSLYSQALGIPTG 378

Query: 305 AHALFPQYRIDMLTLRMKVKDPFS-----FDMF-RFGQAIESTFRSLNNLLEHLHQSFFF 358
            H  F  Y++D +TL++  + P        D F R  + +E T RS+NNLLE  HQSFF 
Sbjct: 379 PHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARLLEGTIRSVNNLLEKFHQSFFL 438

Query: 359 YFILDHLHFISIGNYMPSILILAASFMLGAYRHWI-----------NHEKKIDLWR---- 403
           Y +     FIS+G YM +  +L A   + A   +I           N  +    W+    
Sbjct: 439 YMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKSLTNSTHNPTENFKSWKWLDA 498

Query: 404 ---PFSFWLFSIFCTIAAYYLVS-----SSTKITVFIFL----YLMLTFIGI----IFST 447
               F+  L     T+  Y++       S T  ++         L++TF+ +     FS+
Sbjct: 499 AKQVFTLHLLGFIVTLLPYFICQVPGQHSPTNRSIMWGTTSSSLLLITFVTMPGCSPFSS 558

Query: 448 FMTSEDAELVLSYDLMSKSLFISVVSTLNFSLSFVVAILLVPLQFIS-----FRFNRRLS 502
            +   +  ++ S  + +  + + ++S +NF+ + + A+LLVP   ++        +RRL 
Sbjct: 559 RLHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGALLLVPTCLMAQPIKPALRSRRLK 618

Query: 503 LLFAVLTYFSTFIFLCSLSKILN 525
            L      F + + L     ++ 
Sbjct: 619 SLLGA---FCSIVLLTIGFPVMF 638
>ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila melanogaster]
 gb|AAK77275.1| (AY047543) GH05723p [Drosophila melanogaster]
          Length = 561

 Score =  486 bits (1238), Expect = e-136
 Identities = 122/407 (29%), Positives = 194/407 (46%), Gaps = 30/407 (7%)

Query: 8   QIRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFE---N 64
           Q ++   L RH+  +  +L + G++W F L   E     ++SE+AL PG V        N
Sbjct: 12  QSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPGLVYPEIRIDAN 71

Query: 65  RYSKTVSSSLTAANTWSHLDASVGTNTMYDDLEQIFTAMGLPTQKQNYSINIP---GSEF 121
           R +  +   L       HL     + T +  +       GL T   NY++  P   G E+
Sbjct: 72  RLAIQLLEELQRERK-DHL-----STTPHAWIAAKMNEFGLETHTHNYTLRYPFGGGKEY 125

Query: 122 NGSNFITTLRAPRGDATESLLLCVPWKDHIGQYNE--AGVALAISLLKYFQGWSLWSKDI 179
           +G N    LRAPR  +TE ++   P++     + +  A V L ++   + +  + W+KD+
Sbjct: 126 HGKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKNYWAKDL 185

Query: 180 ILVIFDDPVYGPSSFLTSYF------DQTTPYISYTPLKIRSGSIQAGLSLELVTTENNS 233
           I +I +    G  ++L +Y       D +  Y+    L  R+GS+QA L++E+   E   
Sbjct: 186 IFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAALNIEVQDLE--I 243

Query: 234 DVLEVLYQATNGQLPNLDLFNTISRIFMQHF-NYPLRLQGYDFHANSGSSYTSRLKSLWM 292
           D ++V  +  NG+LPNLD+FN + RI  +       +        +S S +    + +  
Sbjct: 244 DHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHFEQNFRQMLT 303

Query: 293 GMLTQAVSNVTSAHALFPQYRIDMLTL-------RMKVKDPFSFDMFRFGQAIESTFRSL 345
            + +Q+    T  H LF +YRID LT+          +K           +AIE   RSL
Sbjct: 304 MLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLKAIEGIARSL 363

Query: 346 NNLLEHLHQSFFFYFILDHLHFISIGNYMPSILILAASFMLGAYRHW 392
           NNLLE  HQSFFFY I+ +  +ISIG+YMP+++ L A   L AY  W
Sbjct: 364 NNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTW 410
>gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melanogaster]
          Length = 674

 Score =  483 bits (1231), Expect = e-135
 Identities = 122/407 (29%), Positives = 194/407 (46%), Gaps = 30/407 (7%)

Query: 8   QIRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFE---N 64
           Q ++   L RH+  +  +L + G++W F L   E     ++SE+AL PG V        N
Sbjct: 12  QSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPGLVYPEIRIDAN 71

Query: 65  RYSKTVSSSLTAANTWSHLDASVGTNTMYDDLEQIFTAMGLPTQKQNYSINIP---GSEF 121
           R +  +   L       HL     + T +  +       GL T   NY++  P   G E+
Sbjct: 72  RLAIQLLEELQRERK-DHL-----STTPHAWIAAKMNEFGLETHTHNYTLRYPFGGGKEY 125

Query: 122 NGSNFITTLRAPRGDATESLLLCVPWKDHIGQYNE--AGVALAISLLKYFQGWSLWSKDI 179
           +G N    LRAPR  +TE ++   P++     + +  A V L ++   + +  + W+KD+
Sbjct: 126 HGKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKNYWAKDL 185

Query: 180 ILVIFDDPVYGPSSFLTSYF------DQTTPYISYTPLKIRSGSIQAGLSLELVTTENNS 233
           I +I +    G  ++L +Y       D +  Y+    L  R+GS+QA L++E+   E   
Sbjct: 186 IFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAALNIEVQDLE--I 243

Query: 234 DVLEVLYQATNGQLPNLDLFNTISRIFMQHF-NYPLRLQGYDFHANSGSSYTSRLKSLWM 292
           D ++V  +  NG+LPNLD+FN + RI  +       +        +S S +    + +  
Sbjct: 244 DHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHFEQNFRQMLT 303

Query: 293 GMLTQAVSNVTSAHALFPQYRIDMLTL-------RMKVKDPFSFDMFRFGQAIESTFRSL 345
            + +Q+    T  H LF +YRID LT+          +K           +AIE   RSL
Sbjct: 304 MLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLKAIEGIARSL 363

Query: 346 NNLLEHLHQSFFFYFILDHLHFISIGNYMPSILILAASFMLGAYRHW 392
           NNLLE  HQSFFFY I+ +  +ISIG+YMP+++ L A   L AY  W
Sbjct: 364 NNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTW 410
>ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mus musculus]
 dbj|BAA82589.1| (AB002136) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAA82591.1| (AB002138) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAB03274.1| (AB006970) GPI anchor attachment protein [Mus musculus]
 dbj|BAB03275.1| (AB006971) GPI anchor attachment protein [Mus musculus]
 gb|AAH06697.1|AAH06697 (BC006697) GPI anchor attachment protein 1 [Mus musculus]
          Length = 621

 Score =  477 bits (1216), Expect = e-133
 Identities = 124/395 (31%), Positives = 195/395 (48%), Gaps = 26/395 (6%)

Query: 22  LQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFENRYSKTVSSSLTAANTWS 81
           L +   + G++W   L    +  R ++SE+A+     +T  E ++     +   A +  +
Sbjct: 24  LCVLSYVAGIAWFLALAFPPLTQRTYMSENAM----GSTMVEEQFVGGDRARSFARDFAA 79

Query: 82  HLDASVGTNTMYDDLEQIFTAMGLPTQKQNYSINIPGSE-------FNGSNFITTLRAPR 134
           H             LE+   ++GL    Q++S  +P  +        +G+N    LRAPR
Sbjct: 80  HRKKPGALPVA--WLERSMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTNVYGILRAPR 137

Query: 135 GDATESLLLCVPWKDHIGQYNEAGVALAISLLKYFQGWSLWSKDIILVIFDDPVYGPSSF 194
             +TESL+L VP        N   V L ++L  +F+G   W+KDII ++ D  + G  ++
Sbjct: 138 SASTESLVLTVPCGPD--ATNSQAVGLLLALAAHFRGQIYWAKDIIFLVTDHDLLGTEAW 195

Query: 195 LTSYFDQTTPYISYTPLKIRSGSIQAGLSLELVTTENNSDVLEVLYQATNGQLPNLDLFN 254
           L +Y D     I  +PL+ R+G+IQA ++LEL +    S  L+V  +  NGQLPNLDL N
Sbjct: 196 LEAYHDINVTGIQSSPLQGRAGAIQAAVALELSSDVVTS--LDVTVEGLNGQLPNLDLLN 253

Query: 255 TISRIFMQHFNYPLRLQGY----DFHANSGSSYTSRLKSLWMGMLTQAVSNVTSAHALFP 310
            + + F Q       LQG     D+ +  G      L++L + +L QA       H LF 
Sbjct: 254 -LFQTFCQKGGLLCTLQGKLQPQDWTSLEGP--LQGLQTLLLMVLRQASGRPHGPHGLFL 310

Query: 311 QYRIDMLTLR-MKVKDPFSFDMFRFGQAIESTFRSLNNLLEHLHQSFFFYFILDHLHFIS 369
           +Y ++ LTLR +     + +D+   G+A+E  FR LN+LLE LHQSFFFY +     F+S
Sbjct: 311 RYGVEALTLRGINSFRQYKYDLATVGKALEGMFRKLNHLLERLHQSFFFYLLPALSRFVS 370

Query: 370 IGNYMPSILILAASFMLGAYRHWIN-HEKKIDLWR 403
           IG YMP+   L     L A   W+  H+  ++   
Sbjct: 371 IGLYMPATGFLLLVLGLKALELWMQLHQAGVNPEE 405
>ref|NP_003792.1| (NM_003801) anchor attachment protein 1; anchor attachment protein
           1 (Gaa1p, yeast) homolog; glycophosphatidylinositol
           anchor attachment 1 [Homo sapiens]
 dbj|BAA24035.1| (AB006969) hGAA1 [Homo sapiens]
 dbj|BAA82588.1| (AB002135) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Homo sapiens]
 dbj|BAA82590.1| (AB002137) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Homo sapiens]
 gb|AAH03171.1|AAH03171 (BC003171) anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo
           sapiens]
 gb|AAH04129.1|AAH04129 (BC004129) anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo
           sapiens]
          Length = 621

 Score =  466 bits (1188), Expect = e-130
 Identities = 127/408 (31%), Positives = 203/408 (49%), Gaps = 29/408 (7%)

Query: 10  RVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFENRYSKT 69
           R+   L   L    +   + G++W   L    +  R ++SE+A+     +T  E +++  
Sbjct: 15  RLVLRLNAPL---CVLSYVAGIAWFLALVFPPLTQRTYMSENAM----GSTMVEEQFAGG 67

Query: 70  VSSSLTAANTWSHLDASVGTNTMYDDLEQIFTAMGLPTQKQNYSINIPGSE-------FN 122
             +   A +  +H   S         LE+   ++GL    Q++S  +P  +        +
Sbjct: 68  DRARAFARDFAAHRKKSGALPVA--WLERTMRSVGLEVYTQSFSRKLPFPDETHERYMVS 125

Query: 123 GSNFITTLRAPRGDATESLLLCVPWKDHIGQYNEAGVALAISLLKYFQGWSLWSKDIILV 182
           G+N    LRAPR  +TESL+L VP        N   V L ++L  +F+G   W+KDI+ +
Sbjct: 126 GTNVYGILRAPRAASTESLVLTVPCGSD--STNSQAVGLLLALAAHFRGQIYWAKDIVFL 183

Query: 183 IFDDPVYGPSSFLTSYFDQTTPYISYTPLKIRSGSIQAGLSLELVTTENNSDVLEVLYQA 242
           + +  + G  ++L +Y D     +  +PL+ R+G+IQA ++LEL +    S  L+V  + 
Sbjct: 184 VTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTS--LDVAVEG 241

Query: 243 TNGQLPNLDLFNTISRIFMQHFNYPLRLQGY----DFHANSGSSYTSRLKSLWMGMLTQA 298
            NGQLPNLDL N + + F Q       LQG     D+ +  G      L++L + +L QA
Sbjct: 242 LNGQLPNLDLLN-LFQTFCQKGGLLCTLQGKLQPEDWTSLDGP--LQGLQTLLLMVLRQA 298

Query: 299 VSNVTSAHALFPQYRIDMLTLR-MKVKDPFSFDMFRFGQAIESTFRSLNNLLEHLHQSFF 357
                 +H LF +YR++ LTLR +     + +D+   G+A+E  FR LN+LLE LHQSFF
Sbjct: 299 SGRPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQSFF 358

Query: 358 FYFILDHLHFISIGNYMPSILILAASFMLGAYRHWIN-HEKKIDLWRP 404
            Y +     F+SIG YMP++  L     L A   W+  HE  + L  P
Sbjct: 359 LYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEP 406
>pir||T46923 hypothetical protein DKFZp434E1512.1 - human
 emb|CAB75660.1| (AL157437) hypothetical protein [Homo sapiens]
 gb|AAH06383.1|AAH06383 (BC006383) Unknown (protein for MGC:12663) [Homo sapiens]
          Length = 499

 Score =  371 bits (945), Expect = e-101
 Identities = 105/290 (36%), Positives = 157/290 (53%), Gaps = 13/290 (4%)

Query: 121 FNGSNFITTLRAPRGDATESLLLCVPWKDHIGQYNEAGVALAISLLKYFQGWSLWSKDII 180
            +G+N    LRAPR  +TESL+L VP        N   V L ++L  +F+G   W+KDI+
Sbjct: 2   VSGTNVYGILRAPRAASTESLVLTVPCGSD--STNSQAVGLLLALAAHFRGQIYWAKDIV 59

Query: 181 LVIFDDPVYGPSSFLTSYFDQTTPYISYTPLKIRSGSIQAGLSLELVTTENNSDVLEVLY 240
            ++ +  + G  ++L +Y D     +  +PL+ R+G+IQA ++LEL +    S  L+V  
Sbjct: 60  FLVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTS--LDVAV 117

Query: 241 QATNGQLPNLDLFNTISRIFMQHFNYPLRLQGY----DFHANSGSSYTSRLKSLWMGMLT 296
           +  NGQLPNLDL N + + F Q       LQG     D+ +  G      L++L + +L 
Sbjct: 118 EGLNGQLPNLDLLN-LFQTFCQKGGLLCTLQGKLQPEDWTSLDGP--LQGLQTLLLMVLR 174

Query: 297 QAVSNVTSAHALFPQYRIDMLTLR-MKVKDPFSFDMFRFGQAIESTFRSLNNLLEHLHQS 355
           QA      +H LF +YR++ LTLR +     + +D+   G+A+E  FR LN+LLE LHQS
Sbjct: 175 QASGRPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQS 234

Query: 356 FFFYFILDHLHFISIGNYMPSILILAASFMLGAYRHWIN-HEKKIDLWRP 404
           FF Y +     F+SIG YMP++  L     L A   W+  HE  + L  P
Sbjct: 235 FFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEP 284
>ref|NP_491701.1| (NM_059300) F33D11.9a.p [Caenorhabditis elegans]
 pir||T32772 hypothetical protein F33D11.9a - Caenorhabditis elegans
 gb|AAB96702.1| (AF039720) Hypothetical protein F33D11.9a [Caenorhabditis elegans]
          Length = 229

 Score =  211 bits (532), Expect = 4e-53
 Identities = 41/199 (20%), Positives = 73/199 (36%), Gaps = 31/199 (15%)

Query: 372 NYMPSILILAASFMLGAYRHWINHEKKIDLWRPFSFWLFSIFCTIAAYYLVSSSTKITVF 431
            Y+  + IL +  ++ AY  W   ++        +F +   F  IA  Y+    T  T F
Sbjct: 1   MYIIPLTILVSPLVISAYFEWTKIQEFQFPVVHLAFHVLGFFYYIATTYVF--QTIGTTF 58

Query: 432 IFLYLMLTFIGIIFSTFMTSEDAELVLSYDLMSKSLFISVVSTLNFSLSFVVAILLVPLQ 491
           +  ++   F+GI         D +   S+ L++  L   ++              L P  
Sbjct: 59  VLQWMQWPFVGI------DGCDGQFTPSWSLINSFLIFFMIP-------------LGPYV 99

Query: 492 FISFRFNRRLSLLFAVLTYFSTFIFLCSLSKILNGPLVPFWLWAKEYELFNSWLMP--SV 549
           F+    +   S + A+L   S   F+      + G L P           +SWL    S+
Sbjct: 100 FLK-TRSIFRSSVLAILNPISVLAFVAINIPGMFGQLFPTISQ-------DSWLAAAQSI 151

Query: 550 FMILVLPEIIFSVTSFFSL 568
            +  +  ++ F V  FF L
Sbjct: 152 ALTAIREDVEFKVNHFFLL 170
>emb|CAB86709.1| (AL163505) hypothetical protein L3640.14 [Leishmania major]
          Length = 464

 Score = 41.2 bits (96), Expect = 0.051
 Identities = 35/181 (19%), Positives = 59/181 (32%), Gaps = 13/181 (7%)

Query: 220 AGLSLELVTTENNSDVLEVLYQATNGQLPNLDLFNTISRIFMQHFNYPLRLQGYDFHANS 279
           A L L L T  N    + +     NG  PN D  N   ++   +   P  L      +  
Sbjct: 137 AALVLNLSTLYN--RHICIDTLGGNGIQPNQDYTNIAVQLAAANQFLPSYLCQRRHRSTD 194

Query: 280 GSS--YTSRLKSLWMGMLTQAVSNVTSAHALFPQYRIDMLTL------RMKVKDPFSFDM 331
            +   +      L   +  Q  +     H + P + I+ + +            P S   
Sbjct: 195 DAHAGFWHYWAYLEASV--QLPTRPQPWH-IIPTHSINTIAISSDPLYSASSVFPHSQLP 251

Query: 332 FRFGQAIESTFRSLNNLLEHLHQSFFFYFILDHLHFISIGNYMPSILILAASFMLGAYRH 391
             F Q +     SLN L E  H S F +  +    ++   +    I++  AS     Y  
Sbjct: 252 DAFAQLVLKIIVSLNGLEEKFHHSTFVWLPVSLWRYVEYDHAQFCIILFVASIFSTTYSE 311

Query: 392 W 392
           +
Sbjct: 312 Y 312
CPU time:   135.88 user secs.	    2.53 sys. secs	  138.41 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.314    0.167    0.477 

Gapped
Lambda     K      H
   0.270   0.0587    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 336721663
Number of Sequences: 887402
Number of extensions: 15188309
Number of successful extensions: 67341
Number of sequences better than 10.0: 334
Number of HSP's better than 10.0 without gapping: 19
Number of HSP's successfully gapped in prelim test: 318
Number of HSP's that attempted gapping in prelim test: 67058
Number of HSP's gapped (non-prelim): 462
length of query: 581
length of database: 277,845,442
effective HSP length: 55
effective length of query: 526
effective length of database: 229,038,332
effective search space: 120474162632
effective search space used: 120474162632
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.7 bits)
S2: 76 (33.7 bits)