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GPI Anchor Biosynthesis Report: BAC01259.1 (GAA1 family, Oryza sativa (japonica cultivar-group))




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (718 letters)

Database: nr
           1,315,992 sequences; 421,297,171 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 1

Distribution of 17 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

dbj|BAC01259.1| putative GPAA1 - like protein [Oryza sativa (jap... 1081 0.0
gb|AAO00773.1| GPAA1 - like protein [Arabidopsis thaliana] 880 0.0
ref|NP_197414.1| GPAA1 - like protein; protein id: At5g19130.1 [... 871 0.0
ref|NP_034461.1| GPI anchor attachment protein 1 [Mus musculus] ... 600 e-170
ref|NP_003792.1| anchor attachment protein 1; anchor attachment ... 600 e-170
ref|XP_235447.1| similar to GPI anchor attachment protein 1 [Mus... 588 e-166
ref|NP_572273.2| CG3033-PA [Drosophila melanogaster] >gi|7290645... 527 e-148
gb|AAK77275.1| GH05723p [Drosophila melanogaster] 510 e-143
pir||T46923 hypothetical protein DKFZp434E1512.1 - human >gi|701... 498 e-139
ref|NP_593498.1| putative glycosylphosphatidylinositol anchor at... 414 e-114
gb|EAA07058.1| agCP7251 [Anopheles gambiae str. PEST] 387 e-106
ref|NP_491700.2| Putative plasma membrane membrane protein famil... 377 e-103
pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans 370 e-101
ref|NP_013189.1| ER protein essential for attaching GPI (glycosy... 121 6e-26
Alignments
>dbj|BAC01259.1| putative GPAA1 - like protein [Oryza sativa (japonica
           cultivar-group)]
          Length = 718

 Score = 1081 bits (2766), Expect = 0.0
 Identities = 718/718 (100%), Positives = 718/718 (100%)

Query: 1   MASSAKEEAKPKPRLIVRLGVFLASHHILFSAVCCTAGIIALLFLPSLAKNTYLSENALI 60
           MASSAKEEAKPKPRLIVRLGVFLASHHILFSAVCCTAGIIALLFLPSLAKNTYLSENALI
Sbjct: 1   MASSAKEEAKPKPRLIVRLGVFLASHHILFSAVCCTAGIIALLFLPSLAKNTYLSENALI 60

Query: 61  PGSANTLFSTEDVQEANRFAKGIEAAIGESRGGTTEIPKFIAQQTKNLGAEVYYHEFLPD 120
           PGSANTLFSTEDVQEANRFAKGIEAAIGESRGGTTEIPKFIAQQTKNLGAEVYYHEFLPD
Sbjct: 61  PGSANTLFSTEDVQEANRFAKGIEAAIGESRGGTTEIPKFIAQQTKNLGAEVYYHEFLPD 120

Query: 121 SKCFHPLKFFTSMTNNMAAKPNGTYTNFGINTVGIIRAPRGDGKEAIVLVTPYNSQKVTP 180
           SKCFHPLKFFTSMTNNMAAKPNGTYTNFGINTVGIIRAPRGDGKEAIVLVTPYNSQKVTP
Sbjct: 121 SKCFHPLKFFTSMTNNMAAKPNGTYTNFGINTVGIIRAPRGDGKEAIVLVTPYNSQKVTP 180

Query: 181 NELLSLALGFSVFSLLSRAAWLSKDIVWLSADSQFGEYSAVSSWLNQYHNPMFLSHPVNL 240
           NELLSLALGFSVFSLLSRAAWLSKDIVWLSADSQFGEYSAVSSWLNQYHNPMFLSHPVNL
Sbjct: 181 NELLSLALGFSVFSLLSRAAWLSKDIVWLSADSQFGEYSAVSSWLNQYHNPMFLSHPVNL 240

Query: 241 DTKIYGANQILYKPDGTAEKAELMAFKRAGTMAAALIFKVGETRKYGDRDSVTMYAEASN 300
           DTKIYGANQILYKPDGTAEKAELMAFKRAGTMAAALIFKVGETRKYGDRDSVTMYAEASN
Sbjct: 241 DTKIYGANQILYKPDGTAEKAELMAFKRAGTMAAALIFKVGETRKYGDRDSVTMYAEASN 300

Query: 301 GQMPNLDLLNVVHYLAVHRQGFRVNVETFNSLLSSSWLRVIAEVFQNLGSLLRKINPDWK 360
           GQMPNLDLLNVVHYLAVHRQGFRVNVETFNSLLSSSWLRVIAEVFQNLGSLLRKINPDWK
Sbjct: 301 GQMPNLDLLNVVHYLAVHRQGFRVNVETFNSLLSSSWLRVIAEVFQNLGSLLRKINPDWK 360

Query: 361 LDVTVPDYVEGTANLASSMYNQALGVPTGSHGAFRDYQVDAVSLEFAPAFHLKNENAKSS 420
           LDVTVPDYVEGTANLASSMYNQALGVPTGSHGAFRDYQVDAVSLEFAPAFHLKNENAKSS
Sbjct: 361 LDVTVPDYVEGTANLASSMYNQALGVPTGSHGAFRDYQVDAVSLEFAPAFHLKNENAKSS 420

Query: 421 FLLRGGRLTEGVVRSVNNLLEKFHQSFFLYFLTAPSKFISVGVYMIPFALLLAPLPIVAA 480
           FLLRGGRLTEGVVRSVNNLLEKFHQSFFLYFLTAPSKFISVGVYMIPFALLLAPLPIVAA
Sbjct: 421 FLLRGGRLTEGVVRSVNNLLEKFHQSFFLYFLTAPSKFISVGVYMIPFALLLAPLPIVAA 480

Query: 481 ALAGGSKTKGKLEDECKTKGNADDLQMEGGSWKWLKSARVLLIIQFWAVLVSLLPYYISQ 540
           ALAGGSKTKGKLEDECKTKGNADDLQMEGGSWKWLKSARVLLIIQFWAVLVSLLPYYISQ
Sbjct: 481 ALAGGSKTKGKLEDECKTKGNADDLQMEGGSWKWLKSARVLLIIQFWAVLVSLLPYYISQ 540

Query: 541 IPGAMPIQYAVIWAVLSITILIILYAMFGSPSRAGVEWKLLKATMITSITIGMGLMSIIN 600
           IPGAMPIQYAVIWAVLSITILIILYAMFGSPSRAGVEWKLLKATMITSITIGMGLMSIIN
Sbjct: 541 IPGAMPIQYAVIWAVLSITILIILYAMFGSPSRAGVEWKLLKATMITSITIGMGLMSIIN 600

Query: 601 FATAQLGALILIPMCLFSRPLRAQLEMNFLPRTVLLASNILLTVLGFPPAAFLIMKGLSK 660
           FATAQLGALILIPMCLFSRPLRAQLEMNFLPRTVLLASNILLTVLGFPPAAFLIMKGLSK
Sbjct: 601 FATAQLGALILIPMCLFSRPLRAQLEMNFLPRTVLLASNILLTVLGFPPAAFLIMKGLSK 660

Query: 661 GSWTVDIVGDFWLWMEFLWEWSSATYLYVFLVHLPCWLLCIHVLLHPCYQPESKMKQE 718
           GSWTVDIVGDFWLWMEFLWEWSSATYLYVFLVHLPCWLLCIHVLLHPCYQPESKMKQE
Sbjct: 661 GSWTVDIVGDFWLWMEFLWEWSSATYLYVFLVHLPCWLLCIHVLLHPCYQPESKMKQE 718
>gb|AAO00773.1| GPAA1 - like protein [Arabidopsis thaliana]
          Length = 699

 Score =  880 bits (2250), Expect = 0.0
 Identities = 348/708 (49%), Positives = 466/708 (65%), Gaps = 39/708 (5%)

Query: 10  KPKPRLIVRLGVFLASHHILFSAVCCTAGIIALLFLPSLAKNTYLSENALIPGSANTLFS 69
           K KPR IVRLG+FL +H  +FS V   AG++ALL LP LAKNTY+SENAL+PGSA ++ S
Sbjct: 20  KIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSMLS 79

Query: 70  TEDVQEANRFAKGIEAAIGESRGGTTEIPKFIAQQTKNLGAEVYYHEFLPDSKCFHPLKF 129
             DV + ++  K I+       G   E+ K I +   ++GAEV Y +F P+   FHPL F
Sbjct: 80  NRDVSDGSKLVKDIKNFRLNHEGQGVEVQKLIGKYMSDMGAEVSYQKFHPEGNQFHPLHF 139

Query: 130 FTSMTNNMAAKPNGTYTNFGINTVGIIRAPRGDGKEAIVLVTPYNSQKVTPNELLSLALG 189
           F S  ++     N +  ++G+N  GIIRAPRGDGKE+IVLVTPY+       E LSL + 
Sbjct: 140 F-SGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGIV 198

Query: 190 FSVFSLLSRAAWLSKDIVWLSADSQFGEYSAVSSWLNQYHNPMF-LSHPVNLDTKIYGAN 248
            S+FSLLSR  WLSKDI+WL ADS++G+Y  V++WL +YH+P F +S  +  D +    N
Sbjct: 199 SSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCDEQNTADN 258

Query: 249 QILYKPDGTAEKAELMAFKRAGTMAAALIFKV-GETRKYGDRDSVTMYAEASNGQMPNLD 307
                            F+RAGTMAAAL+ KV G + K+   D++++YAEASNGQMPNLD
Sbjct: 259 -----------------FRRAGTMAAALVLKVDGRSEKF--EDTLSIYAEASNGQMPNLD 299

Query: 308 LLNVVHYLAVHRQGFRVNVETFNSLLSSSWLRVIAEVFQNLGSLLRKINPDWKLDVTVPD 367
           L+NVV+YLAVHRQGF V VE   SLLSSSWL++  E+F+ +G L   +NPDW   +   D
Sbjct: 300 LINVVNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIFKAVGKLAHMLNPDWNFGIPAAD 359

Query: 368 YVEGTANLASSMYNQALGVPTGSHGAFRDYQVDAVSLEFAPAFHLKNENAKSSFLLRGGR 427
           Y+EG+A LASS+Y+QALG+PTG HGAFRDYQVDA++L+ +P F   ++  +  F LRG R
Sbjct: 360 YLEGSATLASSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGAR 419

Query: 428 LTEGVVRSVNNLLEKFHQSFFLYFLTAPSKFISVGVYMIPFALLLAPLPIVAAALAGGSK 487
           L EG +RSVNNLLEKFHQSFFLY LT+PSKFISVGVYMI FALL+APLP+VAA+L     
Sbjct: 420 LLEGTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASL----- 474

Query: 488 TKGKLEDECKTKGNADDLQMEG-GSWKWLKSARVLLIIQFWAVLVSLLPYYISQIPGA-M 545
                 D CK+  N+     E   SWKWL +A+ +  +     +V+LLPY+I Q+PG   
Sbjct: 475 ----YIDGCKSLTNSTHNPTENFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQHS 530

Query: 546 PIQYAVIWAVLSITILIILYAMFG--SPSRA---GVEWKLLKATMITSITIGMGLMSIIN 600
           P   +++W   S ++L+I +      SP  +   G  W +LK+  I++  IG+ LMSIIN
Sbjct: 531 PTNRSIMWGTTSSSLLLITFVTMPGCSPFSSRLHGTNWAILKSVTISAAFIGLCLMSIIN 590

Query: 601 FATAQLGALILIPMCLFSRPLRAQLEMNFLPRTVLLASNILLTVLGFPPAAFLIMK-GLS 659
           FATA++GAL+L+P CL ++P++  L    L   +    +I+L  +GFP   F I K  L 
Sbjct: 591 FATAEIGALLLVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGLLG 650

Query: 660 KGSWTVDIVGDFWLWMEFLWEWSSATYLYVFLVHLPCWLLCIHVLLHP 707
           +G   + + G+FW W+E LW W SATYLY+ +VHLPCWLLC+ +L HP
Sbjct: 651 EGLVGLSLGGEFWTWLESLWAWKSATYLYIGMVHLPCWLLCLCILFHP 698
>ref|NP_197414.1| GPAA1 - like protein; protein id: At5g19130.1 [Arabidopsis
           thaliana]
          Length = 696

 Score =  871 bits (2226), Expect = 0.0
 Identities = 347/708 (49%), Positives = 465/708 (65%), Gaps = 42/708 (5%)

Query: 10  KPKPRLIVRLGVFLASHHILFSAVCCTAGIIALLFLPSLAKNTYLSENALIPGSANTLFS 69
           K KPR IVRLG+FL +H  +FS V   AG++ALL LP LAKNTY+SENAL+PGSA ++ S
Sbjct: 20  KIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSMLS 79

Query: 70  TEDVQEANRFAKGIEAAIGESRGGTTEIPKFIAQQTKNLGAEVYYHEFLPDSKCFHPLKF 129
             DV + ++  K I+       G    + K I +   ++GAEV Y +F P+   FHPL F
Sbjct: 80  NRDVSDGSKLVKDIKNFRLNHEGQ---VQKLIGKYMSDMGAEVSYQKFHPEGNQFHPLHF 136

Query: 130 FTSMTNNMAAKPNGTYTNFGINTVGIIRAPRGDGKEAIVLVTPYNSQKVTPNELLSLALG 189
           F S  ++     N +  ++G+N  GIIRAPRGDGKE+IVLVTPY+       E LSL + 
Sbjct: 137 F-SGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGIV 195

Query: 190 FSVFSLLSRAAWLSKDIVWLSADSQFGEYSAVSSWLNQYHNPMF-LSHPVNLDTKIYGAN 248
            S+FSLLSR  WLSKDI+WL ADS++G+Y  V++WL +YH+P F +S  +  D +    N
Sbjct: 196 SSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCDEQNTADN 255

Query: 249 QILYKPDGTAEKAELMAFKRAGTMAAALIFKV-GETRKYGDRDSVTMYAEASNGQMPNLD 307
                            F+RAGTMAAAL+ KV G + K+   D++++YAEASNGQMPNLD
Sbjct: 256 -----------------FRRAGTMAAALVLKVDGRSEKF--EDTLSIYAEASNGQMPNLD 296

Query: 308 LLNVVHYLAVHRQGFRVNVETFNSLLSSSWLRVIAEVFQNLGSLLRKINPDWKLDVTVPD 367
           L+NVV+YLAVHRQGF V VE   SLLSSSWL++  E+F+ +G L   +NPDW   +   D
Sbjct: 297 LINVVNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAAD 356

Query: 368 YVEGTANLASSMYNQALGVPTGSHGAFRDYQVDAVSLEFAPAFHLKNENAKSSFLLRGGR 427
           Y+EG+A LASS+Y+QALG+PTG HGAFRDYQVDA++L+ +P F   ++  +  F LRG R
Sbjct: 357 YLEGSATLASSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGAR 416

Query: 428 LTEGVVRSVNNLLEKFHQSFFLYFLTAPSKFISVGVYMIPFALLLAPLPIVAAALAGGSK 487
           L EG +RSVNNLLEKFHQSFFLY LT+PSKFISVGVYMI FALL+APLP+VAA+L     
Sbjct: 417 LLEGTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASL----- 471

Query: 488 TKGKLEDECKTKGNADDLQMEG-GSWKWLKSARVLLIIQFWAVLVSLLPYYISQIPGA-M 545
                 D CK+  N+     E   SWKWL +A+ +  +     +V+LLPY+I Q+PG   
Sbjct: 472 ----YIDGCKSLTNSTHNPTENFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQHS 527

Query: 546 PIQYAVIWAVLSITILIILYAMFG--SPSRA---GVEWKLLKATMITSITIGMGLMSIIN 600
           P   +++W   S ++L+I +      SP  +   G  W +LK+  I++  IG+ LMSIIN
Sbjct: 528 PTNRSIMWGTTSSSLLLITFVTMPGCSPFSSRLHGTNWAILKSVTISAAFIGLCLMSIIN 587

Query: 601 FATAQLGALILIPMCLFSRPLRAQLEMNFLPRTVLLASNILLTVLGFPPAAFLIMK-GLS 659
           FATA++GAL+L+P CL ++P++  L    L   +    +I+L  +GFP   F I K  L 
Sbjct: 588 FATAEIGALLLVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGLLG 647

Query: 660 KGSWTVDIVGDFWLWMEFLWEWSSATYLYVFLVHLPCWLLCIHVLLHP 707
           +G   + + G+FW W+E LW W SATYLY+ +VHLPCWLLC+ +L HP
Sbjct: 648 EGLVGLSLGGEFWTWLESLWAWKSATYLYIGMVHLPCWLLCLCILFHP 695
>ref|NP_034461.1| GPI anchor attachment protein 1 [Mus musculus]
 dbj|BAA82589.1| glycosylphosphatidylinositol anchor attachment 1 (GPAA1) [Mus
           musculus]
 dbj|BAA82591.1| glycosylphosphatidylinositol anchor attachment 1 (GPAA1) [Mus
           musculus]
 dbj|BAB03274.1| GPI anchor attachment protein [Mus musculus]
 dbj|BAB03275.1| GPI anchor attachment protein [Mus musculus]
 gb|AAH06697.1| GPI anchor attachment protein 1 [Mus musculus]
 dbj|BAC36840.1| unnamed protein product [Mus musculus]
          Length = 621

 Score =  600 bits (1532), Expect = e-170
 Identities = 177/725 (24%), Positives = 270/725 (36%), Gaps = 153/725 (21%)

Query: 14  RLIVRLGVFLASHHILFSAVCCTAGIIALLFLPSLAKNTYLSENALIPGSANTLFSTEDV 73
           R+++RL   L     + S V   A  +AL F P L + TY+SENA+        F   D 
Sbjct: 15  RIVLRLNTPLC----VLSYVAGIAWFLALAF-PPLTQRTYMSENAMGSTMVEEQFVGGD- 68

Query: 74  QEANRFAKGIEAAIGESRGGTTEIP-KFIAQQTKNLGAEVYYHEFLPDSKCFHPLKFFTS 132
             A  FA+   A     R     +P  ++ +  +++G EVY   F         L F   
Sbjct: 69  -RARSFARDFAA----HRKKPGALPVAWLERSMRSVGLEVYTQSF------SRKLPF--- 114

Query: 133 MTNNMAAKPNGTYTNF---GINTVGIIRAPRGDGKEAIVLVTPYNSQKVTPNELLSLALG 189
                   P+ T+  +   G N  GI+RAPR    E++VL  P          +  L   
Sbjct: 115 --------PDETHERYMVSGTNVYGILRAPRSASTESLVLTVPCGPDATNSQAVGLLLAL 166

Query: 190 FSVFSLLSRAAWLSKDIVWLSADSQFGEYSAVSSWLNQYHNPMFLSHPVNLDTKIYGANQ 249
            + F       + +KDI++L  D    +     +WL  YH           D  + G   
Sbjct: 167 AAHF---RGQIYWAKDIIFLVTDH---DLLGTEAWLEAYH-----------DINVTG--- 206

Query: 250 ILYKPDGTAEKAELMAFKRAGTMAAALIFKVGETRKYGDRDSVTMYAEASNGQMPNLDLL 309
                    + + L    RAG + AA+  ++          S+ +  E  NGQ+PNLDLL
Sbjct: 207 --------IQSSPLQG--RAGAIQAAVALELSSDVV----TSLDVTVEGLNGQLPNLDLL 252

Query: 310 NVVHYLAVHRQGFRVNVETFNSLLSSSWLRVIAEVFQNLGSLL----RKINP-DWKLDVT 364
           N+                               + F   G LL     K+ P DW    +
Sbjct: 253 NL------------------------------FQTFCQKGGLLCTLQGKLQPQDW---TS 279

Query: 365 VPDYVEGTANLASSMYNQALGVPTGSHGAFRDYQVDAVSLEFAPAFHLKNENAKSSFLLR 424
           +   ++G   L   +  QA G P G HG F  Y V+A++L    +F     +     L  
Sbjct: 280 LEGPLQGLQTLLLMVLRQASGRPHGPHGLFLRYGVEALTLRGINSFRQYKYD-----LAT 334

Query: 425 GGRLTEGVVRSVNNLLEKFHQSFFLYFLTAPSKFISVGVYMIPFALLLAPLPIVAAALAG 484
            G+  EG+ R +N+LLE+ HQSFF Y L A S+F+S+G+YM     LL  L + A  L  
Sbjct: 335 VGKALEGMFRKLNHLLERLHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKALELWM 394

Query: 485 GSKTKGKL-EDECKTKGNADDLQMEGGSWKWLKSARVLLIIQFWAVLVSLLPYYISQIPG 543
                G   E+  K       L    G          LLI Q   + +  LP     +  
Sbjct: 395 QLHQAGVNPEEAGKAPSPGTPLLPTQG-VGLASLTAPLLISQAMGLALYFLPVLGQHLAT 453

Query: 544 AMPIQYAVIWAVLSITILIILYAMFGS-------------PSRAGVEWKLLKATMITSIT 590
                + V  A   +  L+ +Y    +             P R    W  LK   +  + 
Sbjct: 454 Q---HFPVAEAEAVVLTLLAIYVAGLALPHNTHRVVNSQVPDRG---WMALKLVALIYLA 507

Query: 591 IGMGLMSIINFATAQLGALILIPMCLFSRP-----LRAQLEMNFLPRTVLLASNILLTVL 645
           + +G ++++NF+   L A  ++P    ++P     L A L +   P   L  S  L   L
Sbjct: 508 LQLGCIALLNFSLGFLLAATMVPAAALAKPHGPRTLYAALLVVTSPAVTLFGSLFLWREL 567

Query: 646 GFPPAAF-----LIMKGLSKGSWTVDIVGDFWLWMEFLWEWSSATYLYVFLVHLPCWLLC 700
              P +      L +  L++G       G     +  L  +             PCWLL 
Sbjct: 568 LEVPLSLAEGWQLFLTALAQGVLEHYTYGALLFPILALGLY-------------PCWLLF 614

Query: 701 IHVLL 705
            +VL 
Sbjct: 615 WNVLF 619
>ref|NP_003792.1| anchor attachment protein 1; anchor attachment protein 1 (Gaa1p,
           yeast) homolog; glycophosphatidylinositol anchor
           attachment 1 [Homo sapiens]
 dbj|BAA24035.1| hGAA1 [Homo sapiens]
 dbj|BAA82588.1| glycosylphosphatidylinositol anchor attachment 1 (GPAA1) [Homo
           sapiens]
 dbj|BAA82590.1| glycosylphosphatidylinositol anchor attachment 1 (GPAA1) [Homo
           sapiens]
 gb|AAH03171.1|AAH03171 anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo sapiens]
 gb|AAH04129.1|AAH04129 anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo sapiens]
          Length = 621

 Score =  600 bits (1531), Expect = e-170
 Identities = 179/725 (24%), Positives = 277/725 (37%), Gaps = 153/725 (21%)

Query: 14  RLIVRLGVFLASHHILFSAVCCTAGIIALLFLPSLAKNTYLSENALIPGSANTLFSTEDV 73
           RL++RL   L     + S V   A  +AL+F P L + TY+SENA+        F+    
Sbjct: 15  RLVLRLNAPLC----VLSYVAGIAWFLALVF-PPLTQRTYMSENAMGSTMVEEQFAGG-- 67

Query: 74  QEANRFAKGIEAAIGESRGGTTEIP-KFIAQQTKNLGAEVYYHEFLPDSKCFHPLKFFTS 132
                 A+         R  +  +P  ++ +  +++G EVY   F         L F   
Sbjct: 68  ----DRARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSF------SRKLPF--- 114

Query: 133 MTNNMAAKPNGTYTNF---GINTVGIIRAPRGDGKEAIVLVTPYNSQKVTPNELLSLALG 189
                   P+ T+  +   G N  GI+RAPR    E++VL  P  S       +  L   
Sbjct: 115 --------PDETHERYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLAL 166

Query: 190 FSVFSLLSRAAWLSKDIVWLSADSQFGEYSAVSSWLNQYHNPMFLSHPVNLDTKIYGANQ 249
            + F       + +KDIV+L  +    +     +WL  YH           D  + G   
Sbjct: 167 AAHF---RGQIYWAKDIVFLVTEH---DLLGTEAWLEAYH-----------DVNVTG--- 206

Query: 250 ILYKPDGTAEKAELMAFKRAGTMAAALIFKVGETRKYGDRDSVTMYAEASNGQMPNLDLL 309
                    + + L    RAG + AA+  ++          S+ +  E  NGQ+PNLDLL
Sbjct: 207 --------MQSSPLQG--RAGAIQAAVALELSSDVV----TSLDVAVEGLNGQLPNLDLL 252

Query: 310 NVVHYLAVHRQGFRVNVETFNSLLSSSWLRVIAEVFQNLGSLL----RKINP-DWKLDVT 364
           N+                               + F   G LL     K+ P DW    +
Sbjct: 253 NL------------------------------FQTFCQKGGLLCTLQGKLQPEDW---TS 279

Query: 365 VPDYVEGTANLASSMYNQALGVPTGSHGAFRDYQVDAVSLEFAPAFHLKNENAKSSFLLR 424
           +   ++G   L   +  QA G P GSHG F  Y+V+A++L    +F     +     L+ 
Sbjct: 280 LDGPLQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRGINSFRQYKYD-----LVA 334

Query: 425 GGRLTEGVVRSVNNLLEKFHQSFFLYFLTAPSKFISVGVYMIPFALLLAPLPIVAAALAG 484
            G+  EG+ R +N+LLE+ HQSFFLY L   S+F+S+G+YM     LL  L + A  L  
Sbjct: 335 VGKALEGMFRKLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWM 394

Query: 485 GSKTKGK-LEDECKTKGNADDLQMEGGSWKWLKSARVLLIIQFWAVLVSLLPYYISQIPG 543
                G  LE+     G +  L    G          LLI Q   + + +LP     +  
Sbjct: 395 QLHEAGMGLEEPGGAPGPSVPLPPSQG-VGLASLVAPLLISQAMGLALYVLPVLGQHVAT 453

Query: 544 AMPIQYAVIWAVLSITILIILYAM-------------FGSPSRAGVEWKLLKATMITSIT 590
                + V  A   +  L+ +YA                +P R    W  LK   +  + 
Sbjct: 454 Q---HFPVAEAEAVVLTLLAIYAAGLALPHNTHRVVSTQAPDRG---WMALKLVALIYLA 507

Query: 591 IGMGLMSIINFATAQLGALILIPMCLFSRP-----LRAQLEMNFLPRTVLLASNILLTVL 645
           + +G +++ NF+   L A  ++P    ++P     L A L +   P   LL S  L   L
Sbjct: 508 LQLGCIALTNFSLGFLLATTMVPTAALAKPHGPRTLYAALLVLTSPAATLLGSLFLWREL 567

Query: 646 GFPPAAF-----LIMKGLSKGSWTVDIVGDFWLWMEFLWEWSSATYLYVFLVHLPCWLLC 700
              P +      L +  L++G       G     +  L  +             PCWLL 
Sbjct: 568 QEAPLSLAEGWQLFLAALAQGVLEHHTYGALLFPLLSLGLY-------------PCWLLF 614

Query: 701 IHVLL 705
            +VL 
Sbjct: 615 WNVLF 619
>ref|XP_235447.1| similar to GPI anchor attachment protein 1 [Mus musculus] [Rattus
           norvegicus]
          Length = 671

 Score =  588 bits (1501), Expect = e-166
 Identities = 179/763 (23%), Positives = 273/763 (35%), Gaps = 179/763 (23%)

Query: 14  RLIVRLGVFLASHHILFSAVCCTAGIIALLFLPSLAKNTYLSENALIPGSANTLFSTEDV 73
           R+++RL   L     + S V   A  +AL F P L + TY+SENA+        F   D 
Sbjct: 15  RIVLRLNTPLC----VLSYVAGIAWFLALAF-PPLTQRTYMSENAMGSTMVEEQFVGGD- 68

Query: 74  QEANRFAKGIEAAIGESRGGTTEIP-KFIAQQTKNLGAEVYYHEF---LPDSKCFHPLKF 129
             A  FA+   A     R     +P  ++ +  +++G EVY   F   LP     H    
Sbjct: 69  -RARNFARDFAA----HRKKPGALPVAWLERAMRSVGLEVYTQSFSRKLPFPDETHERYV 123

Query: 130 FTSMTNNMAAKPNGTYTNFGINTVGIIRAPRGDGKEAIVLVTPYNSQKVTPNELLSLALG 189
              M       P G     G N  GI+RAPR    E++VL  P          +  L   
Sbjct: 124 LREM-----GVP-GRKMVSGTNVYGILRAPRAASTESLVLTVPCGPDTTNSQAVGLLLAL 177

Query: 190 FSVFSLLSRAAWLSKDIVWLSADSQFGEYSAVSSWLNQYHNPMFLSHPVNLDTKIYGANQ 249
            + F       + +KDI++L  +    +     +WL  YH           D  + G   
Sbjct: 178 AAHF---RGQIYWAKDIIFLVTEH---DLLGTEAWLEAYH-----------DINVTG--- 217

Query: 250 ILYKPDGTAEKAELMAFKRAGTMAAALIFKVGETRKYGDRDSVTMYAEASNGQMPNLDLL 309
                    + + L    RAG + AA+  ++          S+ +  E  NGQ+PNLDLL
Sbjct: 218 --------IQSSPLQG--RAGAIQAAVALELSSDVV----TSLDVTVEGLNGQLPNLDLL 263

Query: 310 NVVHYLAVHRQGFRVNVETFNSLLSSSWLRVIAEVFQNLGSLL----RKINP-DWKLDVT 364
           N+                               + F   G LL     K+ P DW    +
Sbjct: 264 NL------------------------------FQTFCQKGGLLCTLQGKLQPQDW---TS 290

Query: 365 VPDYVEGTANLASSMYNQALGVPTGSHGAFRDYQVDAVSLEFAPAFHLKNENAKSSFLLR 424
           +   ++G   L   +  QA G P G HG F  Y V+A++L    +F     +     L+ 
Sbjct: 291 LEGPLQGLQTLLLMVLRQASGRPHGPHGLFLRYGVEALTLRGINSFRQYKYD-----LVA 345

Query: 425 GGRLTEGVVRSVNNLLEKFHQSFFLYFLTAPSKFISVGVYMIPFALLLAPLPIVAAA--- 481
            G+  EG+ R +N+LLE+ HQSFF Y L A S+F+S+G+YM     LL  L +  ++   
Sbjct: 346 VGKALEGMFRKLNHLLERLHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKISSTLV 405

Query: 482 ----LAGGS-------KTKGKL-EDECKTKGNADD------------------------L 505
               L   S          G   E+  K  G                            L
Sbjct: 406 LDTHLWPNSDPFNCKLHEAGVNPEEAGKAPGPGSPPLATQVTSYSTALRMGSQRPQQCFL 465

Query: 506 QMEGGSWKWLKSARVLLIIQFWAVLVSLLPYYISQIPGAMPIQYAVIWAVLSITILIILY 565
                S         LLI Q   + +  LP     +       + V  A   +  L+ +Y
Sbjct: 466 WSTLSSVSLASLMAPLLISQAMGLALYFLPVLGQHVAAQ---HFPVAEAEAVVLTLLAIY 522

Query: 566 AMFGS-------------PSRAGVEWKLLKATMITSITIGMGLMSIINFATAQLGALILI 612
               +             P R    W  LK   +  + + +G ++++NF+   L A  ++
Sbjct: 523 VAGLALPHNTHRVVSSQVPDRG---WMALKLVALIYLALQLGCIALLNFSLGFLLAATMV 579

Query: 613 PMCLFSRP-----LRAQLEMNFLPRTVLLASNILLTVLGFPPAAF-----LIMKGLSKGS 662
           P    ++P     L A L +   P   L  S  L   L   P +      L +  L++G 
Sbjct: 580 PAAALTKPHGPRTLYAALLVVTSPAVTLFGSLFLWRELQEVPLSLAEGWQLFLTALAQGV 639

Query: 663 WTVDIVGDFWLWMEFLWEWSSATYLYVFLVHLPCWLLCIHVLL 705
                 G     +  L  +             PCWLL  +VL 
Sbjct: 640 LEHYTYGALLFPILALGLY-------------PCWLLFWNVLF 669
>ref|NP_572273.2| CG3033-PA [Drosophila melanogaster]
 gb|AAF46094.1| CG3033-PA [Drosophila melanogaster]
          Length = 674

 Score =  527 bits (1344), Expect = e-148
 Identities = 169/704 (24%), Positives = 283/704 (40%), Gaps = 127/704 (18%)

Query: 32  AVCCTAGIIALLFLPSLAKNTYLSENALIPGSANTLFSTEDVQEANRFA-KGIEAAIGES 90
            V   A    L  LP     TYLSENAL PG        E   +ANR A + +E    E 
Sbjct: 31  YVAGVAWFFCLA-LPEFNHGTYLSENALSPGLV----YPEIRIDANRLAIQLLEELQRER 85

Query: 91  RGGTTEIPK-FIAQQTKNLGAEVYYHEFLPDSKCFHPLKFFTSMTNNMAAKPNGTYTNF- 148
           +   +  P  +IA +    G E + H +                       P G    + 
Sbjct: 86  KDHLSTTPHAWIAAKMNEFGLETHTHNYTLRY-------------------PFGGGKEYH 126

Query: 149 GINTVGIIRAPRGDGKEAIVLVTPYNSQKVTPNEL---LSLALGFSVFSLLSRAAWLSKD 205
           G N  GI+RAPR    E IV   PY +      ++   + L L F+ F+   R  + +KD
Sbjct: 127 GKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFA--RRKNYWAKD 184

Query: 206 IVWLSADSQFGEYSAVSSWLNQYHNPMFLSHPVNLDTKIYGANQILYKPDGTAEKAELMA 265
           +++L  +    E   + +WL  YH+                 ++ L           L A
Sbjct: 185 LIFLITEQ---EQLGMQAWLEAYHD----------------GDRELDLSKAYLRPGNLPA 225

Query: 266 FKRAGTMAAALIFKVGETRKYGDRDSVTMYAEASNGQMPNLDLLNVVHYLAVHRQGFRVN 325
             RAG++ AAL  +V +       D V +  E  NG++PNLD+ N+V  +        + 
Sbjct: 226 --RAGSLQAALNIEVQDLE----IDHVDVRIEGLNGKLPNLDMFNLVQRI--------MA 271

Query: 326 VETFNSLLSSSWLRVIAEVFQNLGSLLRKINPDWKLDVTVPDYVEGTANLASSMYNQALG 385
            E   S                      K  P  K   +   + +    + + + +Q+ G
Sbjct: 272 REGIASGY--------------------KQAPRKKRRHSQSHFEQNFRQMLTMLASQSSG 311

Query: 386 VPTGSHGAFRDYQVDAVSLEF-APAFHLKNENAKSSFLLRGGRLTEGVVRSVNNLLEKFH 444
           VPTG+HG F  Y++DA+++     A H   + +  S  +   +  EG+ RS+NNLLE+FH
Sbjct: 312 VPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLKAIEGIARSLNNLLERFH 371

Query: 445 QSFFLYFLTAPSKFISVGVYMIPFALLLA-----------PLPIVAAAL--AGGSKTKGK 491
           QSFF Y + +  ++IS+G YM     L+A            LP   A L  A G   + +
Sbjct: 372 QSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLPATNAELEKAAGWLREHE 431

Query: 492 LEDECKTKGNADDLQMEGGSWKWLKSARVLLIIQFWAVLVSLLPYYISQIPGAMPIQYAV 551
            E E +     D  ++  GS     +A   L+I F   ++ L  Y++    GA P+  +V
Sbjct: 432 QEAELEENLEPDKFELPYGSVLIYLTAT--LLIGFLCNVLPLQQYFLEIPMGAAPLTTSV 489

Query: 552 IWAVLSITILIILYAMFGSPSRAGVEWKLLKATMITSITIGMGLMSIINFATAQLGALIL 611
           + + LS+   ++ + +   P   G+E  LL    +      + ++ ++NFA     A++ 
Sbjct: 490 L-SFLSLIGFVLPFVVVLPP--GGLE--LLHVAFLLIYGCALIVIGLLNFALGLFAAVLT 544

Query: 612 IPMCLF--SRPLRAQLEMNFLPRTVLLASNIL---------LTVLGFPPAAFL-IMKGLS 659
           +P+ +   ++   ++  +    R   L  N +         +T   FP      IM   +
Sbjct: 545 VPLVIALETKEENSRSTLRNTIRLATLVMNPMMVVYVIVLAMTFYQFPELPVQKIMLRAA 604

Query: 660 KGSWTVDIVG--DFWLWMEFLWEWSSATYLYVFLVHLPCWLLCI 701
             +      G  D  ++  +L       Y  +  + LP W++C 
Sbjct: 605 TAAMDASAYGLIDSVIYGNWL-------YFVICTIFLPLWIICW 641
>gb|AAK77275.1| GH05723p [Drosophila melanogaster]
          Length = 561

 Score =  510 bits (1300), Expect = e-143
 Identities = 153/605 (25%), Positives = 251/605 (41%), Gaps = 106/605 (17%)

Query: 32  AVCCTAGIIALLFLPSLAKNTYLSENALIPGSANTLFSTEDVQEANRFA-KGIEAAIGES 90
            V   A    L  LP     TYLSENAL PG        E   +ANR A + +E    E 
Sbjct: 31  YVAGVAWFFCLA-LPEFNHGTYLSENALSPGLV----YPEIRIDANRLAIQLLEELQRER 85

Query: 91  RGGTTEIPK-FIAQQTKNLGAEVYYHEFLPDSKCFHPLKFFTSMTNNMAAKPNGTYTNF- 148
           +   +  P  +IA +    G E + H +                       P G    + 
Sbjct: 86  KDHLSTTPHAWIAAKMNEFGLETHTHNYTLRY-------------------PFGGGKEYH 126

Query: 149 GINTVGIIRAPRGDGKEAIVLVTPYNSQKVTPNEL---LSLALGFSVFSLLSRAAWLSKD 205
           G N  GI+RAPR    E IV   PY +      ++   + L L F+ F+   R  + +KD
Sbjct: 127 GKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFA--RRKNYWAKD 184

Query: 206 IVWLSADSQFGEYSAVSSWLNQYHNPMFLSHPVNLDTKIYGANQILYKPDGTAEKAELMA 265
           +++L  +    E   + +WL  YH+                 ++ L           L A
Sbjct: 185 LIFLITEQ---EQLGMQAWLEAYHD----------------GDRELDLSKAYLRPGNLPA 225

Query: 266 FKRAGTMAAALIFKVGETRKYGDRDSVTMYAEASNGQMPNLDLLNVVHYLAVHRQGFRVN 325
             RAG++ AAL  +V +       D V +  E  NG++PNLD+ N+V  +        + 
Sbjct: 226 --RAGSLQAALNIEVQDLE----IDHVDVRIEGLNGKLPNLDMFNLVQRI--------MA 271

Query: 326 VETFNSLLSSSWLRVIAEVFQNLGSLLRKINPDWKLDVTVPDYVEGTANLASSMYNQALG 385
            E   S                      K  P  K   +   + +    + + + +Q+ G
Sbjct: 272 REGIASGY--------------------KQAPRKKRRHSQSHFEQNFRQMLTMLASQSSG 311

Query: 386 VPTGSHGAFRDYQVDAVSLEF-APAFHLKNENAKSSFLLRGGRLTEGVVRSVNNLLEKFH 444
           VPTG+HG F  Y++DA+++     A H   + +  S  +   +  EG+ RS+NNLLE+FH
Sbjct: 312 VPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLKAIEGIARSLNNLLERFH 371

Query: 445 QSFFLYFLTAPSKFISVGVYMIPFALLLA-----------PLPIVAAAL--AGGSKTKGK 491
           QSFF Y + +  ++IS+G YM     L+A            LP   A L  A G   + +
Sbjct: 372 QSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLPATNAELEKAAGWLREHE 431

Query: 492 LEDECKTKGNADDLQMEGGSWKWLKSARVLLIIQFWAVLVSLLPYYISQIPGAMPIQYAV 551
            E E +     D  ++  GS     +A   L+I F   ++ L  Y++    GA P+  +V
Sbjct: 432 QEAELEENLEPDKFELPYGSVLIYLTAT--LLIGFLCNVLPLQQYFLEIPMGAAPLTTSV 489

Query: 552 IWAVLSITILIILYAMFGSPSRAGVEWKLLKATMITSITIGMGLMSIINFATAQLGALIL 611
           + + LS+   ++ + +   P   G+E  LL    +      + ++ ++NFA     A++ 
Sbjct: 490 L-SFLSLIGFVLPFVVVLPP--GGLE--LLHVAFLLIYGCALIVIGLLNFALGLFAAVLT 544

Query: 612 IPMCL 616
           +P+ +
Sbjct: 545 VPLVI 549
>pir||T46923 hypothetical protein DKFZp434E1512.1 - human
 emb|CAB75660.1| hypothetical protein [Homo sapiens]
 gb|AAH06383.1|AAH06383 Unknown (protein for MGC:12663) [Homo sapiens]
          Length = 499

 Score =  498 bits (1270), Expect = e-139
 Identities = 147/589 (24%), Positives = 224/589 (37%), Gaps = 121/589 (20%)

Query: 146 TNFGINTVGIIRAPRGDGKEAIVLVTPYNSQKVTPNELLSLALGFSVFSLLSRAAWLSKD 205
              G N  GI+RAPR    E++VL  P  S       +  L    + F       + +KD
Sbjct: 1   MVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHF---RGQIYWAKD 57

Query: 206 IVWLSADSQFGEYSAVSSWLNQYHNPMFLSHPVNLDTKIYGANQILYKPDGTAEKAELMA 265
           IV+L  +    +     +WL  YH           D  + G            + + L  
Sbjct: 58  IVFLVTEH---DLLGTEAWLEAYH-----------DVNVTG-----------MQSSPLQG 92

Query: 266 FKRAGTMAAALIFKVGETRKYGDRDSVTMYAEASNGQMPNLDLLNVVHYLAVHRQGFRVN 325
             RAG + AA+  ++          S+ +  E  NGQ+PNLDLLN+              
Sbjct: 93  --RAGAIQAAVALELSSDVV----TSLDVAVEGLNGQLPNLDLLNL-------------- 132

Query: 326 VETFNSLLSSSWLRVIAEVFQNLGSLL----RKINP-DWKLDVTVPDYVEGTANLASSMY 380
                            + F   G LL     K+ P DW    ++   ++G   L   + 
Sbjct: 133 ----------------FQTFCQKGGLLCTLQGKLQPEDW---TSLDGPLQGLQTLLLMVL 173

Query: 381 NQALGVPTGSHGAFRDYQVDAVSLEFAPAFHLKNENAKSSFLLRGGRLTEGVVRSVNNLL 440
            QA G P GSHG F  Y+V+A++L    +F     +     L+  G+  EG+ R +N+LL
Sbjct: 174 RQASGRPHGSHGLFLRYRVEALTLRGINSFRQYKYD-----LVAVGKALEGMFRKLNHLL 228

Query: 441 EKFHQSFFLYFLTAPSKFISVGVYMIPFALLLAPLPIVAAALAGGSKTKGK-LEDECKTK 499
           E+ HQSFFLY L   S+F+S+G+YM     LL  L + A  L       G  LE+     
Sbjct: 229 ERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAP 288

Query: 500 GNADDLQMEGGSWKWLKSARVLLIIQFWAVLVSLLPYYISQIPGAMPIQYAVIWAVLSIT 559
           G +  L    G          LLI Q   + + +LP     +       + V  A   + 
Sbjct: 289 GPSVPLPPSQG-VGLASLVAPLLISQAMGLALYVLPVLGQHVATQ---HFPVAEAEAVVL 344

Query: 560 ILIILYAM-------------FGSPSRAGVEWKLLKATMITSITIGMGLMSIINFATAQL 606
            L+ +YA                +P R    W  LK   +  + + +G +++ NF+   L
Sbjct: 345 TLLAIYAAGLALPHNTHRVVSTQAPDRG---WMALKLVALIYLALQLGCIALTNFSLGFL 401

Query: 607 GALILIPMCLFSRP-----LRAQLEMNFLPRTVLLASNILLTVLGFPPAAF-----LIMK 656
            A  ++P    ++P     L A L +   P   LL S  L   L   P +      L + 
Sbjct: 402 LATTMVPTAALAKPHGPRTLYAALLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLA 461

Query: 657 GLSKGSWTVDIVGDFWLWMEFLWEWSSATYLYVFLVHLPCWLLCIHVLL 705
            L++G       G     +  L  +             PCWLL  +VL 
Sbjct: 462 ALAQGVLEHHTYGALLFPLLSLGLY-------------PCWLLFWNVLF 497
>ref|NP_593498.1| putative glycosylphosphatidylinositol anchor attachment protein
           [Schizosaccharomyces pombe]
 emb|CAB65611.1| putative glycosylphosphatidylinositol anchor attachment protein
           [Schizosaccharomyces pombe]
          Length = 581

 Score =  414 bits (1055), Expect = e-114
 Identities = 137/619 (22%), Positives = 245/619 (39%), Gaps = 128/619 (20%)

Query: 17  VRLGVFLASHHILFSAVCCTAGIIALLFLP--SLAKNTYLSENALIPGSANTLFSTEDVQ 74
           +R+  FL  H           G+  +  LP   +    ++SE+AL+PG  NT F      
Sbjct: 9   IRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFE----- 63

Query: 75  EANRFAKGIEAAIGESRGGTTEIPKFIAQQTKNLGAEVYYHEFLPD-SKCFHPLKFFTSM 133
             NR++K + +++              A    +L A V  +    D  + F  +   T  
Sbjct: 64  --NRYSKTVSSSLTA------------ANTWSHLDASVGTNTMYDDLEQIFTAMGLPTQK 109

Query: 134 TNNMAAKPNGTYTNFGINTVGIIRAPRGDGKEAIVLVTPYNSQKVTPNELLSLALGFSVF 193
            N     P   +   G N +  +RAPRGD  E+++L  P+       NE   +AL  S+ 
Sbjct: 110 QNYSINIPGSEFN--GSNFITTLRAPRGDATESLLLCVPWKDHIGQYNE-AGVALAISLL 166

Query: 194 SLLSRAAWLSKDIVWLSADSQ-FGEYSAVSSWLNQYHNPMFLSHPVNLDTKIYGANQILY 252
                 +  SKDI+ +  D   +G  S ++S+ +Q                         
Sbjct: 167 KYFQGWSLWSKDIILVIFDDPVYGPSSFLTSYFDQ------------------------- 201

Query: 253 KPDGTAEKAELMAFK-RAGTMAAALIFKVGETRKYGDRDSVTMYAEASNGQMPNLDLLNV 311
               T         K R+G++ A L  ++  T    + D + +  +A+NGQ+PNLDL N 
Sbjct: 202 ----TTPYISYTPLKIRSGSIQAGLSLELVTTEN--NSDVLEVLYQATNGQLPNLDLFNT 255

Query: 312 VHYLAVHRQGFRVNVETFNSLLSSSWLRVIAEVFQNLGSLLRKINPDWKLDVTVPDYVEG 371
           +  + +    + + ++ +             +   N GS                 Y   
Sbjct: 256 ISRIFMQHFNYPLRLQGY-------------DFHANSGS----------------SYTSR 286

Query: 372 TANLASSMYNQALGVPTGSHGAFRDYQVDAVSLEFAPAFHLKNENAKSSFLLRGGRLTEG 431
             +L   M  QA+   T +H  F  Y++D ++L       +K ++  S  + R G+  E 
Sbjct: 287 LKSLWMGMLTQAVSNVTSAHALFPQYRIDMLTLR------MKVKDPFSFDMFRFGQAIES 340

Query: 432 VVRSVNNLLEKFHQSFFLYFLTAPSKFISVGVYMIPFALLLAPLPIVAAALAGGSKTKGK 491
             RS+NNLLE  HQSFF YF+     FIS+G YM P  L+LA   ++ A     +  K  
Sbjct: 341 TFRSLNNLLEHLHQSFFFYFILDHLHFISIGNYM-PSILILAASFMLGAYRHWINHEK-- 397

Query: 492 LEDECKTKGNADDLQMEGGSWKWLKSARVLLIIQFWAVLVSLLPYYISQIPGAMPIQYAV 551
                        + +      WL S           +  ++  YY+     +   +  V
Sbjct: 398 ------------KIDLWRPFSFWLFS-----------IFCTIAAYYL----VSSSTKITV 430

Query: 552 IWAVLSITILI-ILYAMFGSPSRAGVEWKLLKATMITSITIGMGLMSIINFATAQLGALI 610
              +  +   I I+++ F +   A    +L+ +  + S ++ + ++S +NF+ + + A++
Sbjct: 431 FIFLYLMLTFIGIIFSTFMTSEDA----ELVLSYDLMSKSLFISVVSTLNFSLSFVVAIL 486

Query: 611 LIPMCLFSRPLRAQLEMNF 629
           L+P+   S     +L + F
Sbjct: 487 LVPLQFISFRFNRRLSLLF 505
>gb|EAA07058.1| agCP7251 [Anopheles gambiae str. PEST]
          Length = 647

 Score =  387 bits (985), Expect = e-106
 Identities = 110/417 (26%), Positives = 187/417 (44%), Gaps = 54/417 (12%)

Query: 321 GFRVNVETFNSLLSSSWLRVIAEVFQNLGSLLRKINPDWKLDVTVPDYVEGTANLASSMY 380
              + +E  N  L +  L  + +   +   ++               Y +   NL S ++
Sbjct: 243 HINLKIEGLNGQLPNLDLHNLVQKLSSKNGIIAGYKQSSSSPKRSFRYQDKLLNLLSMVF 302

Query: 381 NQALGVPTGSHGAFRDYQVDAVSLEFAPAFHLKNENAKSSFLLRGG---RLTEGVVRSVN 437
           +QA GVPTG+HG F  Y ++A++LE      +K E  +       G   R+ EG+ RS+N
Sbjct: 303 SQASGVPTGNHGLFHKYGIEALTLEA-----VKRERTQQQQFQEVGSMLRIIEGICRSLN 357

Query: 438 NLLEKFHQSFFLYFLTAPSKFISVGVYMIPFALLLAPLPIVA------AALAGGSKTKGK 491
           NLLE+FHQSFF Y L +  +F+S+G YM   AL+   L I A          G +    K
Sbjct: 358 NLLERFHQSFFFYLLVSNDRFVSIGDYMPSLALMAGALLIKAFIHYLSLYYTGDNDETEK 417

Query: 492 LE--DECKTKGNADDLQMEGGSWKWLKSARVLLIIQFWAVLVSLLPY------YISQIPG 543
            E  D  +TK  A ++ +      +     VLL+      LV LLP       Y+ +   
Sbjct: 418 SEETDAVETKQKAGNIDI-----GYFSVGIVLLVAHTLGALVVLLPQSKAVSNYLHE--A 470

Query: 544 AMPIQYAVIWAVLSITILIILYAMFGSPSRAGVEWKLLKATMITSITIGMGLMSIINFAT 603
            +  Q+ +    L + +L++   +    S +G+  + L   ++  +   +  + ++NF+ 
Sbjct: 471 ELSTQFGLF--SLFVAVLVVALTLPAFCSLSGINGEALHVAVLLELGTVLLAVGMLNFSL 528

Query: 604 AQLGALILIPMCLFSRPLRAQLEMNFLPRTVLLASNIL---------LTVLGFPPAAF-- 652
               +++L+P  +  RP  ++  M FL R   L  N L         LT   FP  AF  
Sbjct: 529 GFSLSVLLVPFIILIRPAASRGAM-FLSRLCCLLINPLVVLYCVLLALTWYLFPELAFKP 587

Query: 653 LIMKGLSK--GSWTVDIVGDFWLWMEFLWEWSSATYLYVFLVHLPCWLLCIHVLLHP 707
           ++ K L+    + T  +V D  ++  +L++  S       L+ +PCW+L + VLL P
Sbjct: 588 MLNKALTATMDALTFSVV-DSMIYGNWLFDMVS-------LIFVPCWVL-LWVLLFP 635
 Score =  238 bits (603), Expect = 3e-61
 Identities = 77/297 (25%), Positives = 118/297 (38%), Gaps = 68/297 (22%)

Query: 31  SAVCCTAGIIALLFLPSLAK-----NTYLSENALIPGSANTLFSTEDVQEANRFAKGIEA 85
           +A+C T  ++ + F  +L        TY SENAL+PG  N+    E V       K + +
Sbjct: 27  TAICLTLYLLGVGFFCALPDANFNSGTYFSENALLPGLVNSELEMETVN----LVKSLSS 82

Query: 86  AIGESRG---GTTEIPKFIAQQTKNLGAEVYYHEFLPDSKCFHPLKFFTSMTNNMAAKPN 142
            +   R         P ++  + +  G E + H F  +                    P 
Sbjct: 83  ELQRERENYPKGMPYP-WLLAKMRRFGLETHTHNFTLNY-------------------PF 122

Query: 143 GTYTNF-GINTVGIIRAPRGDGKEAIVLVTPYNSQKVTPNEL---LSLALGFSVFSLLSR 198
           G    F G N  GI+RAPR    EAIV+  PY   +    ++   + L L F+ F+   R
Sbjct: 123 GGGKRFKGENVYGILRAPRIASTEAIVISVPYRPPETVHTDVSAGVPLMLAFADFA--RR 180

Query: 199 AAWLSKDIVWLSADSQFGEYSAVSSWLNQYHNPMFLSHPVNLDTKIYGANQILYKPDGTA 258
             + +KDI++L  +    E   + +WL  YH                G ++      G  
Sbjct: 181 KKYWAKDIIFLVTEQ---EQLGMQAWLEAYH----------------GGDEGRILDSGLL 221

Query: 259 EKAELMAFKRAGTMAAALIFKVGETRKYGDRDSVTMYAEASNGQMPNLDLLNVVHYL 315
                    RAG++ AAL  +V       D   + +  E  NGQ+PNLDL N+V  L
Sbjct: 222 RA-------RAGSIQAALNLEVQSL----DISHINLKIEGLNGQLPNLDLHNLVQKL 267
>ref|NP_491700.2| Putative plasma membrane membrane protein family member, with at
           least 7 transmembrane domains, of eukaryotic origin
           [Caenorhabditis elegans]
 gb|AAK68360.2|AF039720_13 Hypothetical protein F33D11.9b [Caenorhabditis elegans]
          Length = 676

 Score =  377 bits (958), Expect = e-103
 Identities = 131/649 (20%), Positives = 231/649 (35%), Gaps = 143/649 (22%)

Query: 43  LFLPSLAKNTYLSENALIPGSANTLFSTED--VQEANRFAKGIEAAIGESRGGTTEIPKF 100
           LF P   + T +SE++L+PG     F      +Q   RF               +++PK 
Sbjct: 58  LFDPVNMEVTRISEHSLMPGLVTPKFDKSGIAIQLYRRF---------------SDLPKS 102

Query: 101 IAQQT------KNLGAEVYYHEFLPDSKCFHPLKFFTSMTNNMAAKPNGTYTNFGINTVG 154
            +QQ        +LG E + H++                 + +A  P       G N  G
Sbjct: 103 KSQQELIHTIFSDLGLECFTHKW----------------RSKVAGNPMN-----GENVYG 141

Query: 155 IIRAPRGDGKEAIVLVTPYNSQKVTPNELLSLALGFSVFSLLSRAAWLSKDIVWLSADSQ 214
            IR PR DG EA ++V      + +   ++S  L F  ++      + ++DIV +  D  
Sbjct: 142 FIRGPRNDGAEAQMIVVQLGRSEKS-RRMMSRMLAFVDYA--KDQVYWARDIVIVFVDG- 197

Query: 215 FGEYS--------AVSSWLNQYHNPMFLSHPVNLDTKIYGANQILYKPDGTAEKAELMAF 266
            GE +        A+ ++L +Y              KI   N    K   T E  E+ A 
Sbjct: 198 -GEKNDSIEKAAFALDAFLLKYQ-------------KIEALNS---KKSITVEADEIQA- 239

Query: 267 KRAGTMAAALIFKVGETRKYGDRDSVTMYAEASNGQMPNLDLLNVVHYLAVHRQGFRVNV 326
            + G +   +++ +      G +  V +     NGQ  NLD+ N +  +A  +   RV +
Sbjct: 240 -QTGALIGGVVYDLSGMAVKG-QHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHSRVAI 297

Query: 327 ETFNSLLSSSWLRVIAEVFQNLGSLLRKINPDWKLDVTVPDYVEGTANLA--SSMYNQAL 384
                                 G+L R  NP          Y+E +  +    ++Y QA 
Sbjct: 298 ---------------------FGTLHRHSNP----------YMEYSPFIVPMRALYTQAF 326

Query: 385 GVPTGSHGAFRDYQVDAVSLEFAPAFHLKNENAKSSFLLRGGRLTEGVVRSVNNLLEKFH 444
               G H     Y V  +++  +  +  K          + G+  E V R +NN+LE+ H
Sbjct: 327 ISIEGIHSVLGKYGVQGLTVGLSHDYSEK----------QTGQFIEEVSRMLNNVLERLH 376

Query: 445 QSFFLYFLTAPSKFISVGVYMIPFALLLAPLPIVAAALAGGSKTKGKLEDECKTKGNADD 504
           QS+F+Y L     F S+ +Y+IP  +L++PL I A       +                 
Sbjct: 377 QSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQEFQF---------PVVH 427

Query: 505 LQMEGGSWKWLKSARVLLIIQFWAVLVSLL----PYY-ISQIPGAMPIQYAVIWAVL-SI 558
           L        +        + Q       L     P+  I    G     +++I + L   
Sbjct: 428 L--AFHVLGFFYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSWSLINSFLIFF 485

Query: 559 TILIILYAMFGSPSRAGVEWKLLKATMITSITIGMGLMSIINFATAQLGALILIPMC-LF 617
            I +  Y     P R       ++   +   ++ +  + + NF  A L    ++P+  L 
Sbjct: 486 MIPLGPYVFLKEPVRCVPS---MRIVCLLETSLVLYGICLTNFGLAILCTAFVVPIVFLM 542

Query: 618 SRPLRAQLEMNFLPRTVLLA---SNILLTVLGFPPAAFLIMKGLSKGSW 663
           +     +    F    + +    S +    +  P     +   +S+ SW
Sbjct: 543 TYESSDRTRSIFRSSVLAILNPISVLAFVAINIPGMFGQLFPTISQDSW 591
>pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans
          Length = 885

 Score =  370 bits (941), Expect = e-101
 Identities = 107/459 (23%), Positives = 178/459 (38%), Gaps = 119/459 (25%)

Query: 43  LFLPSLAKNTYLSENALIPGSANTLFSTED--VQEANRFAKGIEAAIGESRGGTTEIPKF 100
           LF P   + T +SE++L+PG     F      +Q   RF               +++PK 
Sbjct: 320 LFDPVNMEVTRISEHSLMPGLVTPKFDKSGIAIQLYRRF---------------SDLPKS 364

Query: 101 IAQQT------KNLGAEVYYHEFLPDSKCFHPLKFFTSMTNNMAAKPNGTYTNFGINTVG 154
            +QQ        +LG E + H++                 + +A  P       G N  G
Sbjct: 365 KSQQELIHTIFSDLGLECFTHKW----------------RSKVAGNPMN-----GENVYG 403

Query: 155 IIRAPRGDGKEAIVLVTPYNSQKVTPNELLSLALGFSVFSLLSRAAWLSKDIVWLSADSQ 214
            IR PR DG EA ++V      + +   ++S  L F  ++      + ++DIV +  D  
Sbjct: 404 FIRGPRNDGAEAQMIVVQLGRSEKS-RRMMSRMLAFVDYA--KDQVYWARDIVIVFVDG- 459

Query: 215 FGEYS--------AVSSWLNQYHNPMFLSHPVNLDTKIYGANQILYKPDGTAEKAELMAF 266
            GE +        A+ ++L +Y              KI   N    K   T E  E+ A 
Sbjct: 460 -GEKNDSIEKAAFALDAFLLKYQ-------------KIEALNS---KKSITVEADEIQA- 501

Query: 267 KRAGTMAAALIFKVGETRKYGDRDSVTMYAEASNGQMPNLDLLNVVHYLAVHRQGFRVNV 326
            + G +   +++ +      G +  V +     NGQ  NLD+ N +  +A  +   RV +
Sbjct: 502 -QTGALIGGVVYDLSGMAVKG-QHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHSRVAI 559

Query: 327 ETFNSLLSSSWLRVIAEVFQNLGSLLRKINPDWKLDVTVPDYVEGTANLA--SSMYNQAL 384
                                 G+L R  NP          Y+E +  +    ++Y QA 
Sbjct: 560 ---------------------FGTLHRHSNP----------YMEYSPFIVPMRALYTQAF 588

Query: 385 GVPTGSHGAFRDYQVDAVSLEFAPAFHLKNENAKSSFLLRGGRLTEGVVRSVNNLLEKFH 444
               G H     Y V  +++  +  +  K          + G+  E V R +NN+LE+ H
Sbjct: 589 ISIEGIHSVLGKYGVQGLTVGLSHDYSEK----------QTGQFIEEVSRMLNNVLERLH 638

Query: 445 QSFFLYFLTAPSKFISVGVYMIPFALLLAPLPIVAAALA 483
           QS+F+Y L     F S+ +Y+IP  +L++PL I A    
Sbjct: 639 QSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEW 677
>ref|NP_013189.1| ER protein essential for attaching GPI
           (glycosylphosphatidylinositol) to protein; Gaa1p
           [Saccharomyces cerevisiae]
 sp|P39012|GAA1_YEAST GAA1 PROTEIN
 pir||S45053 membrane protein END2 - yeast (Saccharomyces cerevisiae)
 emb|CAA55944.1| Gaa1 [Saccharomyces cerevisiae]
 gb|AAB67592.1| Gaa1p [Saccharomyces cerevisiae]
 emb|CAA97649.1| ORF YLR088w [Saccharomyces cerevisiae]
          Length = 614

 Score =  121 bits (302), Expect = 6e-26
 Identities = 61/289 (21%), Positives = 107/289 (36%), Gaps = 35/289 (12%)

Query: 328 TFNSLLSSSWLRVIAEVFQNLGSLLRKINPDWKLDVTVPDYVEGTANLASSMYNQALGVP 387
             N  L +  L  IA        +   ++      +T  ++      L   + + AL   
Sbjct: 227 GLNGELPNLDLVNIAISITEHEGMKVSLHGLPSDQLTNNNFWSRLKILCLGIRDWALSGV 286

Query: 388 TGSHG--AFRDYQVDAVSLEFAPAFHLKNENAKSSFLLRGGRLTEGVVRSVNNLLEKFHQ 445
              HG  AF  +++ +V+L+     H  + +  ++F    GR+ E + RS+NNLLEKFHQ
Sbjct: 287 KKPHGNEAFSGWRIQSVTLKA----HGNSGHDITTF----GRIPEAMFRSINNLLEKFHQ 338

Query: 446 SFFLYFLTAPSKFISVGVYMIPFALLLAPLPIVAAALAGGSKTKGKLEDECKTKGNADDL 505
           SFF Y L AP +F+S+  Y+     L     I +      +                  L
Sbjct: 339 SFFFYLLLAPRQFVSISSYLPSAVALSIAFAISSLNAFINNAYANIS------------L 386

Query: 506 QMEGGSWKWLKSARVLLIIQFWAVLVSLLPYYISQIPGAMPIQYAVIWAVLSITILIILY 565
             E      L    V  +    + +VS    ++      + +  ++    L + +   ++
Sbjct: 387 FSEYNLVALL----VWFVSLVISFVVS--QAFLLIPSSGLLMTISMASCFLPLILSRKIH 440

Query: 566 AMFGSPSRAGVEWKLLKATMITSITIGMGLMSIINFATAQLGALILIPM 614
                  R       LK       ++    + +INFA A L   +  PM
Sbjct: 441 ISEPLSYR-------LKNVAFLYFSLVSTSLLMINFAMALLIGTLAFPM 482
 Score =  100 bits (249), Expect = 1e-19
 Identities = 50/229 (21%), Positives = 90/229 (38%), Gaps = 38/229 (16%)

Query: 10  KPKPRLI-VRLGVFLASHHILFSAVCCTAGIIALLFLPSLAK--NTYLSENALIPGSANT 66
           K   R++ + L   + +   + S +C   G I++  LP   +   TY+SENAL+P  A +
Sbjct: 6   KLHRRIVDMGLVPRIIALLPVISMLCALFGFISIAILPMDGQYRRTYISENALMPSQAYS 65

Query: 67  LFSTEDVQEANRFAKGIEAAIGESRGGTTEIPKFIAQQTKNLGAEVYYHEFLPDSKCFHP 126
            F   +      +   I+  +                   ++        +L +      
Sbjct: 66  YFRESEWNILRGYRSQIKEMVN----------------MTSMERNNLMGSWLQEFGTKTA 109

Query: 127 LKFFTSMTNNMAAKPNGTYTNFGINTVGIIRAPRGDGKEAIVLVTPYNSQKVTPNELLSL 186
           +                    +G    G++ APRGDG EA+VL  P+ +     N +   
Sbjct: 110 IY---------------ENEQYGETLYGVMHAPRGDGTEAMVLAVPWFNSDDEFN-IGGA 153

Query: 187 ALGFSVFSLLSRAAWLSKDIVWLSADSQFGEYSAVSSWLNQYHNPMFLS 235
           ALG S+    SR    SK+I+ + +++      A+ SW+  YH  + L+
Sbjct: 154 ALGVSLARFFSRWPVWSKNIIVVFSENPRA---ALRSWVEAYHTSLDLT 199
 Score = 48.7 bits (115), Expect = 5e-04
 Identities = 18/78 (23%), Positives = 40/78 (51%), Gaps = 4/78 (5%)

Query: 270 GTMAAALIFKVGETRKYGDRDSVTMYAEASNGQMPNLDLLNVVHYLAVHRQGFRVNVETF 329
           G++ AA++     T  + +   V +  +  NG++PNLDL+N+   +  H +G +V++   
Sbjct: 201 GSIEAAVVLDYSSTEDFFE--YVEISYDGLNGELPNLDLVNIAISITEH-EGMKVSLHGL 257

Query: 330 NS-LLSSSWLRVIAEVFQ 346
            S  L+++      ++  
Sbjct: 258 PSDQLTNNNFWSRLKILC 275
CPU time:   173.63 user secs.	    3.15 sys. secs	  176.78 total secs.

  Database: nr
    Posted date:  Jan 18, 2003  1:23 PM
  Number of letters in database: 421,297,171
  Number of sequences in database:  1,315,992
  
Lambda     K      H
   0.312    0.156    0.436 

Gapped
Lambda     K      H
   0.270   0.0548    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 476379381
Number of Sequences: 1315992
Number of extensions: 16496079
Number of successful extensions: 64382
Number of sequences better than 10.0: 443
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 429
Number of HSP's that attempted gapping in prelim test: 64063
Number of HSP's gapped (non-prelim): 501
length of query: 718
length of database: 421,297,171
effective HSP length: 58
effective length of query: 660
effective length of database: 344,969,635
effective search space: 227679959100
effective search space used: 227679959100
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.2 bits)
S2: 78 (34.6 bits)