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GPI Anchor Biosynthesis Report: T39916 (DPM2 family, Schizosaccharomyces pombe)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (72 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!

 Results of PSI-Blast iteration 2

Distribution of 8 Blast Hits on the Query Sequence

Sequences with E-value BETTER than threshold (0.002)


Sequences producing significant alignments:


 pir||T39916  dpm2-like protein - fission yeast (Schizosaccharomyc...    92  1e-18

 ref|NP_177574.1|  (NM_106094) putative dolichyl-phosphate mannosy...    89  1e-17

 sp|Q9Z1P1|DPM2_CRIGR  DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REG...    81  2e-15

 ref|NP_003854.1|  (NM_003863) dolichyl-phosphate mannosyltransfer...    78  2e-14

 ref|NP_034203.1|  (NM_010073) dolichol-phosphate (beta-D) mannosy...    74  5e-13

 ref|NP_062125.1|  (NM_019252) dolichol-phosphate (beta-D) mannosy...    72  1e-12


Sequences with E-value WORSE than threshold 


 gb|AAH15374.1|AAH15374  (BC015374) Similar to dolichyl-phosphate ...    35  0.16

 sp|Q90617|LMP2_CHICK  LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 2...    30  6.6


Alignments


>pir||T39916 dpm2-like protein - fission yeast (Schizosaccharomyces pombe)
 emb|CAB57919.1| (AL121794) dpm2-like protein [Schizosaccharomyces pombe]
          Length = 72

 Score = 91.8 bits (226), Expect = 1e-18
 Identities = 72/72 (100%), Positives = 72/72 (100%)

Query: 1  MIVYISTAAFLYYTIWVLIMPFVDNMNISQKLFLDREWAITIPVAVMLFGICLIGTFVSL 60
          MIVYISTAAFLYYTIWVLIMPFVDNMNISQKLFLDREWAITIPVAVMLFGICLIGTFVSL
Sbjct: 1  MIVYISTAAFLYYTIWVLIMPFVDNMNISQKLFLDREWAITIPVAVMLFGICLIGTFVSL 60

Query: 61 LMIKSSKKKSDL 72
          LMIKSSKKKSDL
Sbjct: 61 LMIKSSKKKSDL 72





>ref|NP_177574.1| (NM_106094) putative dolichyl-phosphate mannosyltransferase
          polypeptide 2 [Arabidopsis thaliana]
 gb|AAG52357.1|AC011765_9 (AC011765) putative dolichyl-phosphate mannosyltransferase
          polypeptide 2; 4974-4608 [Arabidopsis thaliana]
          Length = 80

 Score = 88.7 bits (218), Expect = 1e-17
 Identities = 29/70 (41%), Positives = 47/70 (66%)

Query: 1  MIVYISTAAFLYYTIWVLIMPFVDNMNISQKLFLDREWAITIPVAVMLFGICLIGTFVSL 60
          ++  IS + F YYT WV+I+PFVD+ +   K FL +++AI +PV   +  + LI  F+ +
Sbjct: 11 LLSSISLSIFTYYTFWVIILPFVDSDHFIHKYFLPQDYAILVPVFAGIALLSLISVFIGM 70

Query: 61 LMIKSSKKKS 70
          +M+KS KKK+
Sbjct: 71 VMLKSKKKKA 80





>sp|Q9Z1P1|DPM2_CRIGR DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN
 gb|AAD04344.1| (AF115410) Dpm2p [Cricetulus griseus]
 gb|AAD38194.1| (AF155144) DPM2 protein [Cricetulus griseus]
          Length = 84

 Score = 81.3 bits (199), Expect = 2e-15
 Identities = 25/66 (37%), Positives = 44/66 (65%)

Query: 2  IVYISTAAFLYYTIWVLIMPFVDNMNISQKLFLDREWAITIPVAVMLFGICLIGTFVSLL 61
          +V +S   F YYT WV+++PF+D+ ++  K FL R +A+ IP+A  L  +  +G F++ +
Sbjct: 13 LVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLIPLATGLLLLLFVGLFITYV 72

Query: 62 MIKSSK 67
          M+KS +
Sbjct: 73 MLKSRR 78





>ref|NP_003854.1| (NM_003863) dolichyl-phosphate mannosyltransferase polypeptide 2;
          dolichol phosphate-mannose synthesis 2 [Homo sapiens]
 sp|O94777|DPM2_HUMAN Dolichol phosphate-mannose biosynthesis regulatory protein
 dbj|BAA33974.1| (AB013361) DPM2 [Homo sapiens]
 gb|AAG43140.1|AF061729_1 (AF061729) My026 protein [Homo sapiens]
          Length = 84

 Score = 78.2 bits (191), Expect = 2e-14
 Identities = 25/66 (37%), Positives = 44/66 (65%)

Query: 2  IVYISTAAFLYYTIWVLIMPFVDNMNISQKLFLDREWAITIPVAVMLFGICLIGTFVSLL 61
          +V +S   F YYT WV+++PF+D+ ++  K FL R +A+ IP+A  L  +  +G F+S +
Sbjct: 13 LVAVSLIIFTYYTAWVILLPFIDSQHVIHKYFLPRAYAVAIPLAAGLLLLLFVGLFISYV 72

Query: 62 MIKSSK 67
          M+K+ +
Sbjct: 73 MLKTKR 78





>ref|NP_034203.1| (NM_010073) dolichol-phosphate (beta-D) mannosyltransferase 2
          [Mus musculus]
 sp|Q9Z324|DPM2_MOUSE Dolichol phosphate-mannose biosynthesis regulatory protein
 dbj|BAA33973.1| (AB013360) DPM2 [Mus musculus]
 dbj|BAB22122.1| (AK002468) data source:MGD, source key:MGI:1330238,
          evidence:ISS~dolichol-phosphate (beta-D)
          mannosyltransferase 2~putative [Mus musculus]
 dbj|BAB23234.1| (AK004241) data source:MGD, source key:MGI:1330238,
          evidence:ISS~dolichol-phosphate (beta-D)
          mannosyltransferase 2~putative [Mus musculus]
 gb|AAH08256.1|AAH08256 (BC008256) dolichol-phosphate (beta-D) mannosyltransferase 2 [Mus
          musculus]
          Length = 84

 Score = 73.5 bits (179), Expect = 5e-13
 Identities = 25/66 (37%), Positives = 44/66 (65%)

Query: 2  IVYISTAAFLYYTIWVLIMPFVDNMNISQKLFLDREWAITIPVAVMLFGICLIGTFVSLL 61
          +V +S   F YYT WV+++PF+D+ ++  K FL R +A+ +P+A  L  +  +G F++ +
Sbjct: 13 LVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLLLLLFVGLFITYV 72

Query: 62 MIKSSK 67
          M+KS K
Sbjct: 73 MLKSQK 78





>ref|NP_062125.1| (NM_019252) dolichol-phosphate (beta-D) mannosyltransferase 2
          [Rattus norvegicus]
 sp|Q9Z325|DPM2_RAT DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN
 dbj|BAA33972.1| (AB013359) DPM2 [Rattus norvegicus]
          Length = 84

 Score = 72.0 bits (175), Expect = 1e-12
 Identities = 24/66 (36%), Positives = 44/66 (66%)

Query: 2  IVYISTAAFLYYTIWVLIMPFVDNMNISQKLFLDREWAITIPVAVMLFGICLIGTFVSLL 61
          +V +S   F YYT WV+++PF+D+ ++  K FL R +A+ +P+A  L  +  +G F++ +
Sbjct: 13 LVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLLLLLFVGLFITYV 72

Query: 62 MIKSSK 67
          ++KS K
Sbjct: 73 LLKSQK 78





>gb|AAH15374.1|AAH15374 (BC015374) Similar to dolichyl-phosphate mannosyltransferase
          polypeptide 2, regulatory subunit [Homo sapiens]
          Length = 68

 Score = 35.0 bits (80), Expect = 0.16
 Identities = 8/19 (42%), Positives = 13/19 (68%)

Query: 2  IVYISTAAFLYYTIWVLIM 20
          +V +S   F YYT WV+++
Sbjct: 13 LVAVSLIIFTYYTAWVILL 31





>sp|Q90617|LMP2_CHICK LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 2 PRECURSOR (LAMP-2)
 gb|AAA99540.1| (U10547) lysosome-associated membrane glycoprotein LAMP-2b [Gallus
           gallus]
          Length = 425

 Score = 29.9 bits (67), Expect = 6.6
 Identities = 17/60 (28%), Positives = 31/60 (51%), Gaps = 6/60 (10%)

Query: 13  YTIWVLIMPFVDNMN---ISQKLFLDREWAITIPVAVML-FGICLIGTFVSLLMIKSSKK 68
           + +WV   PF+   N   I+++ F D +    IPVAV +  G  +I  F+S ++ +   +
Sbjct: 362 FNLWV--QPFLVKENKFSIAEECFADSDLNFLIPVAVGMALGFLIILVFISYIIGRRKSR 419





CPU time:    35.37 user secs.	    1.19 sys. secs	   36.56 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.328    0.170    0.553 

Gapped
Lambda     K      H
   0.270   0.0597    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54072441
Number of Sequences: 887402
Number of extensions: 2436059
Number of successful extensions: 20585
Number of sequences better than 10.0: 122
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 108
Number of HSP's that attempted gapping in prelim test: 20542
Number of HSP's gapped (non-prelim): 130
length of query: 72
length of database: 277,845,442
effective HSP length: 47
effective length of query: 25
effective length of database: 236,137,548
effective search space: 5903438700
effective search space used: 5903438700
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 39 (21.0 bits)
S2: 65 (29.4 bits)