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GPI Anchor Biosynthesis Report: NP_177574.1 (DPM2 family, Arabidopsis thaliana)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (80 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!

 Results of PSI-Blast iteration 2

Distribution of 7 Blast Hits on the Query Sequence

Sequences with E-value BETTER than threshold (0.002)


Sequences producing significant alignments:


 ref|NP_177574.1|  (NM_106094) putative dolichyl-phosphate mannosy...   100  6e-21

 sp|Q9Z1P1|DPM2_CRIGR  DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REG...    95  2e-19

 pir||T39916  dpm2-like protein - fission yeast (Schizosaccharomyc...    91  2e-18

 ref|NP_003854.1|  (NM_003863) dolichyl-phosphate mannosyltransfer...    91  3e-18

 ref|NP_034203.1|  (NM_010073) dolichol-phosphate (beta-D) mannosy...    86  8e-17

 ref|NP_062125.1|  (NM_019252) dolichol-phosphate (beta-D) mannosy...    84  3e-16

 gb|AAH15374.1|AAH15374  (BC015374) Similar to dolichyl-phosphate ...    47  5e-05


Alignments


>ref|NP_177574.1| (NM_106094) putative dolichyl-phosphate mannosyltransferase
          polypeptide 2 [Arabidopsis thaliana]
 gb|AAG52357.1|AC011765_9 (AC011765) putative dolichyl-phosphate mannosyltransferase
          polypeptide 2; 4974-4608 [Arabidopsis thaliana]
          Length = 80

 Score =  100 bits (247), Expect = 6e-21
 Identities = 80/80 (100%), Positives = 80/80 (100%)

Query: 1  MELADRAVGLLLSSISLSIFTYYTFWVIILPFVDSDHFIHKYFLPQDYAILVPVFAGIAL 60
          MELADRAVGLLLSSISLSIFTYYTFWVIILPFVDSDHFIHKYFLPQDYAILVPVFAGIAL
Sbjct: 1  MELADRAVGLLLSSISLSIFTYYTFWVIILPFVDSDHFIHKYFLPQDYAILVPVFAGIAL 60

Query: 61 LSLISVFIGMVMLKSKKKKA 80
          LSLISVFIGMVMLKSKKKKA
Sbjct: 61 LSLISVFIGMVMLKSKKKKA 80





>sp|Q9Z1P1|DPM2_CRIGR DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN
 gb|AAD04344.1| (AF115410) Dpm2p [Cricetulus griseus]
 gb|AAD38194.1| (AF155144) DPM2 protein [Cricetulus griseus]
          Length = 84

 Score = 94.6 bits (233), Expect = 2e-19
 Identities = 42/73 (57%), Positives = 56/73 (76%)

Query: 5  DRAVGLLLSSISLSIFTYYTFWVIILPFVDSDHFIHKYFLPQDYAILVPVFAGIALLSLI 64
          D+ VG  L ++SL IFTYYT WVI+LPF+DS H IHKYFLP+ YA+L+P+  G+ LL  +
Sbjct: 6  DQVVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLIPLATGLLLLLFV 65

Query: 65 SVFIGMVMLKSKK 77
           +FI  VMLKS++
Sbjct: 66 GLFITYVMLKSRR 78





>pir||T39916 dpm2-like protein - fission yeast (Schizosaccharomyces pombe)
 emb|CAB57919.1| (AL121794) dpm2-like protein [Schizosaccharomyces pombe]
          Length = 72

 Score = 91.5 bits (225), Expect = 2e-18
 Identities = 29/69 (42%), Positives = 46/69 (66%)

Query: 12 LSSISLSIFTYYTFWVIILPFVDSDHFIHKYFLPQDYAILVPVFAGIALLSLISVFIGMV 71
          +  IS + F YYT WV+I+PFVD+ +   K FL +++AI +PV   +  + LI  F+ ++
Sbjct: 2  IVYISTAAFLYYTIWVLIMPFVDNMNISQKLFLDREWAITIPVAVMLFGICLIGTFVSLL 61

Query: 72 MLKSKKKKA 80
          M+KS KKK+
Sbjct: 62 MIKSSKKKS 70





>ref|NP_003854.1| (NM_003863) dolichyl-phosphate mannosyltransferase polypeptide 2;
          dolichol phosphate-mannose synthesis 2 [Homo sapiens]
 sp|O94777|DPM2_HUMAN Dolichol phosphate-mannose biosynthesis regulatory protein
 dbj|BAA33974.1| (AB013361) DPM2 [Homo sapiens]
 gb|AAG43140.1|AF061729_1 (AF061729) My026 protein [Homo sapiens]
          Length = 84

 Score = 91.1 bits (224), Expect = 3e-18
 Identities = 43/73 (58%), Positives = 57/73 (77%)

Query: 5  DRAVGLLLSSISLSIFTYYTFWVIILPFVDSDHFIHKYFLPQDYAILVPVFAGIALLSLI 64
          D+ VGL L ++SL IFTYYT WVI+LPF+DS H IHKYFLP+ YA+ +P+ AG+ LL  +
Sbjct: 6  DQVVGLGLVAVSLIIFTYYTAWVILLPFIDSQHVIHKYFLPRAYAVAIPLAAGLLLLLFV 65

Query: 65 SVFIGMVMLKSKK 77
           +FI  VMLK+K+
Sbjct: 66 GLFISYVMLKTKR 78





>ref|NP_034203.1| (NM_010073) dolichol-phosphate (beta-D) mannosyltransferase 2
          [Mus musculus]
 sp|Q9Z324|DPM2_MOUSE Dolichol phosphate-mannose biosynthesis regulatory protein
 dbj|BAA33973.1| (AB013360) DPM2 [Mus musculus]
 dbj|BAB22122.1| (AK002468) data source:MGD, source key:MGI:1330238,
          evidence:ISS~dolichol-phosphate (beta-D)
          mannosyltransferase 2~putative [Mus musculus]
 dbj|BAB23234.1| (AK004241) data source:MGD, source key:MGI:1330238,
          evidence:ISS~dolichol-phosphate (beta-D)
          mannosyltransferase 2~putative [Mus musculus]
 gb|AAH08256.1|AAH08256 (BC008256) dolichol-phosphate (beta-D) mannosyltransferase 2 [Mus
          musculus]
          Length = 84

 Score = 86.0 bits (211), Expect = 8e-17
 Identities = 45/73 (61%), Positives = 58/73 (78%)

Query: 5  DRAVGLLLSSISLSIFTYYTFWVIILPFVDSDHFIHKYFLPQDYAILVPVFAGIALLSLI 64
          D+AVG  L ++SL IFTYYT WVI+LPF+DS H IHKYFLP+ YA+L+P+ AG+ LL  +
Sbjct: 6  DQAVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLLLLLFV 65

Query: 65 SVFIGMVMLKSKK 77
           +FI  VMLKS+K
Sbjct: 66 GLFITYVMLKSQK 78





>ref|NP_062125.1| (NM_019252) dolichol-phosphate (beta-D) mannosyltransferase 2
          [Rattus norvegicus]
 sp|Q9Z325|DPM2_RAT DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN
 dbj|BAA33972.1| (AB013359) DPM2 [Rattus norvegicus]
          Length = 84

 Score = 84.5 bits (207), Expect = 3e-16
 Identities = 44/73 (60%), Positives = 58/73 (79%)

Query: 5  DRAVGLLLSSISLSIFTYYTFWVIILPFVDSDHFIHKYFLPQDYAILVPVFAGIALLSLI 64
          D+AVG  L ++SL IFTYYT WVI+LPF+DS H IHKYFLP+ YA+L+P+ AG+ LL  +
Sbjct: 6  DQAVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLLLLLFV 65

Query: 65 SVFIGMVMLKSKK 77
           +FI  V+LKS+K
Sbjct: 66 GLFITYVLLKSQK 78





>gb|AAH15374.1|AAH15374 (BC015374) Similar to dolichyl-phosphate mannosyltransferase
          polypeptide 2, regulatory subunit [Homo sapiens]
          Length = 68

 Score = 47.1 bits (111), Expect = 5e-05
 Identities = 17/26 (65%), Positives = 21/26 (80%)

Query: 5  DRAVGLLLSSISLSIFTYYTFWVIIL 30
          D+ VGL L ++SL IFTYYT WVI+L
Sbjct: 6  DQVVGLGLVAVSLIIFTYYTAWVILL 31





CPU time:    37.70 user secs.	    1.18 sys. secs	   38.88 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.328    0.167    0.533 

Gapped
Lambda     K      H
   0.270   0.0587    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57546341
Number of Sequences: 887402
Number of extensions: 2626612
Number of successful extensions: 15941
Number of sequences better than 10.0: 146
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 133
Number of HSP's that attempted gapping in prelim test: 15898
Number of HSP's gapped (non-prelim): 160
length of query: 80
length of database: 277,845,442
effective HSP length: 48
effective length of query: 32
effective length of database: 235,250,146
effective search space: 7528004672
effective search space used: 7528004672
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.5 bits)
S2: 66 (29.8 bits)