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GPI Anchor Biosynthesis Report: DPM2_MOUSE (DPM2 family, Mus musculus)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (84 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!

 Results of PSI-Blast iteration 1

Distribution of 7 Blast Hits on the Query Sequence

Sequences with E-value BETTER than threshold (0.002)


Sequences producing significant alignments:


 sp|Q9Z1P1|DPM2_CRIGR  DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REG...   108  2e-23

 ref|NP_003854.1|  (NM_003863) dolichyl-phosphate mannosyltransfer...   105  1e-22

 ref|NP_034203.1|  (NM_010073) dolichol-phosphate (beta-D) mannosy...   101  2e-21

 ref|NP_062125.1|  (NM_019252) dolichol-phosphate (beta-D) mannosy...   100  6e-21

 ref|NP_177574.1|  (NM_106094) putative dolichyl-phosphate mannosy...    91  3e-18

 pir||T39916  dpm2-like protein - fission yeast (Schizosaccharomyc...    88  3e-17

 gb|AAH15374.1|AAH15374  (BC015374) Similar to dolichyl-phosphate ...    55  2e-07


Alignments


>sp|Q9Z1P1|DPM2_CRIGR DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN
 gb|AAD04344.1| (AF115410) Dpm2p [Cricetulus griseus]
 gb|AAD38194.1| (AF155144) DPM2 protein [Cricetulus griseus]
          Length = 84

 Score =  108 bits (269), Expect = 2e-23
 Identities = 78/84 (92%), Positives = 82/84 (96%)

Query: 1  MATGTDQAVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLL 60
          MATGTDQ VGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVL+PLA GLL
Sbjct: 1  MATGTDQVVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLIPLATGLL 60

Query: 61 LLLFVGLFITYVMLKSQKITKKAQ 84
          LLLFVGLFITYVMLKS+++TKKAQ
Sbjct: 61 LLLFVGLFITYVMLKSRRLTKKAQ 84





>ref|NP_003854.1| (NM_003863) dolichyl-phosphate mannosyltransferase polypeptide 2;
          dolichol phosphate-mannose synthesis 2 [Homo sapiens]
 sp|O94777|DPM2_HUMAN Dolichol phosphate-mannose biosynthesis regulatory protein
 dbj|BAA33974.1| (AB013361) DPM2 [Homo sapiens]
 gb|AAG43140.1|AF061729_1 (AF061729) My026 protein [Homo sapiens]
          Length = 84

 Score =  105 bits (261), Expect = 1e-22
 Identities = 74/84 (88%), Positives = 80/84 (95%)

Query: 1  MATGTDQAVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLL 60
          MATGTDQ VG GLVAVSLIIFTYYT WVILLPFIDSQHVIHKYFLPRAYAV +PLAAGLL
Sbjct: 1  MATGTDQVVGLGLVAVSLIIFTYYTAWVILLPFIDSQHVIHKYFLPRAYAVAIPLAAGLL 60

Query: 61 LLLFVGLFITYVMLKSQKITKKAQ 84
          LLLFVGLFI+YVMLK++++TKKAQ
Sbjct: 61 LLLFVGLFISYVMLKTKRVTKKAQ 84





>ref|NP_034203.1| (NM_010073) dolichol-phosphate (beta-D) mannosyltransferase 2
          [Mus musculus]
 sp|Q9Z324|DPM2_MOUSE Dolichol phosphate-mannose biosynthesis regulatory protein
 dbj|BAA33973.1| (AB013360) DPM2 [Mus musculus]
 dbj|BAB22122.1| (AK002468) data source:MGD, source key:MGI:1330238,
          evidence:ISS~dolichol-phosphate (beta-D)
          mannosyltransferase 2~putative [Mus musculus]
 dbj|BAB23234.1| (AK004241) data source:MGD, source key:MGI:1330238,
          evidence:ISS~dolichol-phosphate (beta-D)
          mannosyltransferase 2~putative [Mus musculus]
 gb|AAH08256.1|AAH08256 (BC008256) dolichol-phosphate (beta-D) mannosyltransferase 2 [Mus
          musculus]
          Length = 84

 Score =  101 bits (251), Expect = 2e-21
 Identities = 84/84 (100%), Positives = 84/84 (100%)

Query: 1  MATGTDQAVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLL 60
          MATGTDQAVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLL
Sbjct: 1  MATGTDQAVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLL 60

Query: 61 LLLFVGLFITYVMLKSQKITKKAQ 84
          LLLFVGLFITYVMLKSQKITKKAQ
Sbjct: 61 LLLFVGLFITYVMLKSQKITKKAQ 84





>ref|NP_062125.1| (NM_019252) dolichol-phosphate (beta-D) mannosyltransferase 2
          [Rattus norvegicus]
 sp|Q9Z325|DPM2_RAT DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN
 dbj|BAA33972.1| (AB013359) DPM2 [Rattus norvegicus]
          Length = 84

 Score =  100 bits (247), Expect = 6e-21
 Identities = 82/84 (97%), Positives = 84/84 (99%)

Query: 1  MATGTDQAVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLL 60
          MATGTDQAVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLL
Sbjct: 1  MATGTDQAVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLL 60

Query: 61 LLLFVGLFITYVMLKSQKITKKAQ 84
          LLLFVGLFITYV+LKSQK+TKKAQ
Sbjct: 61 LLLFVGLFITYVLLKSQKVTKKAQ 84





>ref|NP_177574.1| (NM_106094) putative dolichyl-phosphate mannosyltransferase
          polypeptide 2 [Arabidopsis thaliana]
 gb|AAG52357.1|AC011765_9 (AC011765) putative dolichyl-phosphate mannosyltransferase
          polypeptide 2; 4974-4608 [Arabidopsis thaliana]
          Length = 80

 Score = 91.5 bits (225), Expect = 3e-18
 Identities = 45/73 (61%), Positives = 58/73 (78%)

Query: 6  DQAVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLLLLLFV 65
          D+AVG  L ++SL IFTYYT WVI+LPF+DS H IHKYFLP+ YA+L+P+ AG+ LL  +
Sbjct: 5  DRAVGLLLSSISLSIFTYYTFWVIILPFVDSDHFIHKYFLPQDYAILVPVFAGIALLSLI 64

Query: 66 GLFITYVMLKSQK 78
           +FI  VMLKS+K
Sbjct: 65 SVFIGMVMLKSKK 77





>pir||T39916 dpm2-like protein - fission yeast (Schizosaccharomyces pombe)
 emb|CAB57919.1| (AL121794) dpm2-like protein [Schizosaccharomyces pombe]
          Length = 72

 Score = 88.0 bits (216), Expect = 3e-17
 Identities = 25/66 (37%), Positives = 44/66 (65%)

Query: 13 LVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLLLLLFVGLFITYV 72
          +V +S   F YYT WV+++PF+D+ ++  K FL R +A+ +P+A  L  +  +G F++ +
Sbjct: 2  IVYISTAAFLYYTIWVLIMPFVDNMNISQKLFLDREWAITIPVAVMLFGICLIGTFVSLL 61

Query: 73 MLKSQK 78
          M+KS K
Sbjct: 62 MIKSSK 67





>gb|AAH15374.1|AAH15374 (BC015374) Similar to dolichyl-phosphate mannosyltransferase
          polypeptide 2, regulatory subunit [Homo sapiens]
          Length = 68

 Score = 55.2 bits (132), Expect = 2e-07
 Identities = 28/31 (90%), Positives = 28/31 (90%)

Query: 1  MATGTDQAVGFGLVAVSLIIFTYYTTWVILL 31
          MATGTDQ VG GLVAVSLIIFTYYT WVILL
Sbjct: 1  MATGTDQVVGLGLVAVSLIIFTYYTAWVILL 31





CPU time:    34.19 user secs.	    1.19 sys. secs	   35.38 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.333    0.168    0.546 

Gapped
Lambda     K      H
   0.270   0.0588    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52851483
Number of Sequences: 887402
Number of extensions: 2132542
Number of successful extensions: 11122
Number of sequences better than 10.0: 107
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 97
Number of HSP's that attempted gapping in prelim test: 11101
Number of HSP's gapped (non-prelim): 111
length of query: 84
length of database: 277,845,442
effective HSP length: 48
effective length of query: 36
effective length of database: 235,250,146
effective search space: 8469005256
effective search space used: 8469005256
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.8 bits)
S2: 66 (29.8 bits)