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GPI Anchor Biosynthesis Report: DPM2_CRIGR (DPM2 family, Cricetulus griseus)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (84 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!

 Results of PSI-Blast iteration 1

Distribution of 7 Blast Hits on the Query Sequence

Sequences with E-value BETTER than threshold (0.002)


Sequences producing significant alignments:


 sp|Q9Z1P1|DPM2_CRIGR  DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REG...   108  2e-23

 ref|NP_003854.1|  (NM_003863) dolichyl-phosphate mannosyltransfer...   105  2e-22

 ref|NP_034203.1|  (NM_010073) dolichol-phosphate (beta-D) mannosy...   101  3e-21

 ref|NP_062125.1|  (NM_019252) dolichol-phosphate (beta-D) mannosy...   100  8e-21

 ref|NP_177574.1|  (NM_106094) putative dolichyl-phosphate mannosy...    91  4e-18

 pir||T39916  dpm2-like protein - fission yeast (Schizosaccharomyc...    88  4e-17

 gb|AAH15374.1|AAH15374  (BC015374) Similar to dolichyl-phosphate ...    55  3e-07


Alignments


>sp|Q9Z1P1|DPM2_CRIGR DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN
 gb|AAD04344.1| (AF115410) Dpm2p [Cricetulus griseus]
 gb|AAD38194.1| (AF155144) DPM2 protein [Cricetulus griseus]
          Length = 84

 Score =  108 bits (268), Expect = 2e-23
 Identities = 84/84 (100%), Positives = 84/84 (100%)

Query: 1  MATGTDQVVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLIPLATGLL 60
          MATGTDQVVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLIPLATGLL
Sbjct: 1  MATGTDQVVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLIPLATGLL 60

Query: 61 LLLFVGLFITYVMLKSRRLTKKAQ 84
          LLLFVGLFITYVMLKSRRLTKKAQ
Sbjct: 61 LLLFVGLFITYVMLKSRRLTKKAQ 84





>ref|NP_003854.1| (NM_003863) dolichyl-phosphate mannosyltransferase polypeptide 2;
          dolichol phosphate-mannose synthesis 2 [Homo sapiens]
 sp|O94777|DPM2_HUMAN Dolichol phosphate-mannose biosynthesis regulatory protein
 dbj|BAA33974.1| (AB013361) DPM2 [Homo sapiens]
 gb|AAG43140.1|AF061729_1 (AF061729) My026 protein [Homo sapiens]
          Length = 84

 Score =  105 bits (260), Expect = 2e-22
 Identities = 76/84 (90%), Positives = 80/84 (94%)

Query: 1  MATGTDQVVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLIPLATGLL 60
          MATGTDQVVG GLVAVSLIIFTYYT WVILLPFIDSQHVIHKYFLPRAYAV IPLA GLL
Sbjct: 1  MATGTDQVVGLGLVAVSLIIFTYYTAWVILLPFIDSQHVIHKYFLPRAYAVAIPLAAGLL 60

Query: 61 LLLFVGLFITYVMLKSRRLTKKAQ 84
          LLLFVGLFI+YVMLK++R+TKKAQ
Sbjct: 61 LLLFVGLFISYVMLKTKRVTKKAQ 84





>ref|NP_034203.1| (NM_010073) dolichol-phosphate (beta-D) mannosyltransferase 2
          [Mus musculus]
 sp|Q9Z324|DPM2_MOUSE Dolichol phosphate-mannose biosynthesis regulatory protein
 dbj|BAA33973.1| (AB013360) DPM2 [Mus musculus]
 dbj|BAB22122.1| (AK002468) data source:MGD, source key:MGI:1330238,
          evidence:ISS~dolichol-phosphate (beta-D)
          mannosyltransferase 2~putative [Mus musculus]
 dbj|BAB23234.1| (AK004241) data source:MGD, source key:MGI:1330238,
          evidence:ISS~dolichol-phosphate (beta-D)
          mannosyltransferase 2~putative [Mus musculus]
 gb|AAH08256.1|AAH08256 (BC008256) dolichol-phosphate (beta-D) mannosyltransferase 2 [Mus
          musculus]
          Length = 84

 Score =  101 bits (250), Expect = 3e-21
 Identities = 78/84 (92%), Positives = 82/84 (96%)

Query: 1  MATGTDQVVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLIPLATGLL 60
          MATGTDQ VGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVL+PLA GLL
Sbjct: 1  MATGTDQAVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLL 60

Query: 61 LLLFVGLFITYVMLKSRRLTKKAQ 84
          LLLFVGLFITYVMLKS+++TKKAQ
Sbjct: 61 LLLFVGLFITYVMLKSQKITKKAQ 84





>ref|NP_062125.1| (NM_019252) dolichol-phosphate (beta-D) mannosyltransferase 2
          [Rattus norvegicus]
 sp|Q9Z325|DPM2_RAT DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN
 dbj|BAA33972.1| (AB013359) DPM2 [Rattus norvegicus]
          Length = 84

 Score = 99.6 bits (246), Expect = 8e-21
 Identities = 77/84 (91%), Positives = 82/84 (96%)

Query: 1  MATGTDQVVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLIPLATGLL 60
          MATGTDQ VGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVL+PLA GLL
Sbjct: 1  MATGTDQAVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLL 60

Query: 61 LLLFVGLFITYVMLKSRRLTKKAQ 84
          LLLFVGLFITYV+LKS+++TKKAQ
Sbjct: 61 LLLFVGLFITYVLLKSQKVTKKAQ 84





>ref|NP_177574.1| (NM_106094) putative dolichyl-phosphate mannosyltransferase
          polypeptide 2 [Arabidopsis thaliana]
 gb|AAG52357.1|AC011765_9 (AC011765) putative dolichyl-phosphate mannosyltransferase
          polypeptide 2; 4974-4608 [Arabidopsis thaliana]
          Length = 80

 Score = 91.1 bits (224), Expect = 4e-18
 Identities = 42/73 (57%), Positives = 56/73 (76%)

Query: 6  DQVVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLIPLATGLLLLLFV 65
          D+ VG  L ++SL IFTYYT WVI+LPF+DS H IHKYFLP+ YA+L+P+  G+ LL  +
Sbjct: 5  DRAVGLLLSSISLSIFTYYTFWVIILPFVDSDHFIHKYFLPQDYAILVPVFAGIALLSLI 64

Query: 66 GLFITYVMLKSRR 78
           +FI  VMLKS++
Sbjct: 65 SVFIGMVMLKSKK 77





>pir||T39916 dpm2-like protein - fission yeast (Schizosaccharomyces pombe)
 emb|CAB57919.1| (AL121794) dpm2-like protein [Schizosaccharomyces pombe]
          Length = 72

 Score = 87.6 bits (215), Expect = 4e-17
 Identities = 25/66 (37%), Positives = 44/66 (65%)

Query: 13 LVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLIPLATGLLLLLFVGLFITYV 72
          +V +S   F YYT WV+++PF+D+ ++  K FL R +A+ IP+A  L  +  +G F++ +
Sbjct: 2  IVYISTAAFLYYTIWVLIMPFVDNMNISQKLFLDREWAITIPVAVMLFGICLIGTFVSLL 61

Query: 73 MLKSRR 78
          M+KS +
Sbjct: 62 MIKSSK 67





>gb|AAH15374.1|AAH15374 (BC015374) Similar to dolichyl-phosphate mannosyltransferase
          polypeptide 2, regulatory subunit [Homo sapiens]
          Length = 68

 Score = 54.9 bits (131), Expect = 3e-07
 Identities = 29/31 (93%), Positives = 29/31 (93%)

Query: 1  MATGTDQVVGFGLVAVSLIIFTYYTTWVILL 31
          MATGTDQVVG GLVAVSLIIFTYYT WVILL
Sbjct: 1  MATGTDQVVGLGLVAVSLIIFTYYTAWVILL 31





CPU time:    31.28 user secs.	    1.05 sys. secs	   32.33 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.333    0.168    0.546 

Gapped
Lambda     K      H
   0.270   0.0588    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52851242
Number of Sequences: 887402
Number of extensions: 2132537
Number of successful extensions: 15213
Number of sequences better than 10.0: 110
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 100
Number of HSP's that attempted gapping in prelim test: 15179
Number of HSP's gapped (non-prelim): 116
length of query: 84
length of database: 277,845,442
effective HSP length: 47
effective length of query: 37
effective length of database: 236,137,548
effective search space: 8737089276
effective search space used: 8737089276
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 39 (21.3 bits)
S2: 66 (29.8 bits)