Tumor Endothelial Marker Report: TEM5 (scaffold + Rho GEF)

	TEM domain structure

	TEM Sequence Analysis

name	from	to	source/E	description
unknown	1	517	BLASTP without filtering	No known domain matches this region. It is LCR-rich (P) and 8 potential SH3 binding motif sequences (PXXP) cluster in the region. A potential 14-3-3-binding motif (RSXSXP) is found at position 179-184. The actin-binding protein profilin binds polyproline rich sequence tracts containing two or more ZPPX motifs in a stretch of no more than 100 residues (Z- P G A or charged, X- preferentially hydrophobic). The N-terminal of TEM5old4 contains 7 ZPPX motifs (64, 102, 343, 422, 428, 483, 490), 4 with a hydrophobic amino-acid at motif-position 4 (64, 102, 342, 421, 427). The function of the proline-rich N-terminal region remains unknown, although a partially sequence-similar proline-rich region in FGD1 was shown to mediate subcellular localization to the actin cytoskeleton.
	9	20	SEG	LCR(SL)
	30	55	predator	HELIX
	57	68	SEG	LCR(DPG)
	162	176	SEG	LCR(GS)
	277	344	SEG25	LCR(PSGE)
	478	502	SEG	LCR(PA)
DH/RhoGEF	517	700	PFAM, E=7.5e-43	This region contains a potential GEF-domain for Rho/Rac/Cdc42-like GTPases. DH domains are found to function not only as GEFs, but additionally to recruit GTPases to targets and locations. The DH-domain of TEM5 shows closest homology to bcr, ect2, pbl (the latter two are Rho-specific).
PH	718	930	IMPALA1.1, E=3.3 (722-822)	The hit is not reliable, but as PH domains are invariably found C-terminally to DH domains it can be considered. The assignment is based additionaly on a Psi-Blast search of the region 700-1000 with the inclusion condition E=0.002 collecting the PH domain of human bcr. While the DH domain is responsible for catalytic activity, the PH domain has proposed functions in proper localization or full catalytic activity of the DH domain.
unknown	930	1012	44% identity in CG7397	This region of unknown function contains a motif of potential significance due to its 100% preserved sequence compared to CG7397: VWVCNSDGYVGQVC
	1012	1048	SEG	No known domain matches this region. It is LCR enriched in proline and alanine.
	1083	1134	SEG	No known domain matches this region. It is LCR enriched in serine and contains a predicted high scoring PEST domain.
apolar region	1135	1155	TOPPREDM(1.22)	The following 7bladedbeta-propeler domain seems to be bordered by two apolar, unstructured regions.
7 bladed beta-propeler	1191	1362	IMPALA-SCOP, E= 1e-06	The region is predicted to form a 7 bladed beta-propeler, which is a structure frequently involved in multiple protein-interaction formation. The secondary structures adopted in this region can be described are predicted as follows: alpha-helix (1251-1239) & 7predicted beta-str (1239-1362). The region shows homology to the last 400 aa of JNK/SAPK-associated protein-1 (see below).
apolar region	1380	1394	TOPPREDM(1.539)	The preceeding 7bladedbeta-propeler domain seems to be bordered by two apolar, unstructured regions.
unknown	1394	1493	blastp	This region shows homology to CG7397 and JNK/SAPK-associated protein-1, the later of the two being more indicative on its potential function as it is studied. Seven potential SH3 binding motif sequences (PXXP) cluster in this region.
1510

	Remarks

• CLASSIFICATION: TEM5 is a DH family member Rho GEF and syd-related protein. The DH family comprises a number of proteins that share two conserved domains - a catalytic DH domain of approximately 250 amino acids, and a pleckstrin homology (PH) domain of approximately 100 amino acids.

• HOMOLOGUES: The characteristic domain organization combining a Rho-GEF and a seven propeller scaffolding domain is found in : CG7397 gene product [Drosophila], KIAA0294 [Homo sapiens], KIAA1626 protein [Homo sapiens],CG8557 gene product [Drosophila]. All these are not studied.

• LOCALIZATION: The region between 250 to 750 shows similarity to FGD1, a protein containing a PH, DH domain and a N-terminal proline rich region. FGD1 is known to localize to the subcortical actin cytoskeleton via its proline-rich N-terminal domain.
• DOMAIN ARCHITECTURE & FUNCTION: TEM5 potentially links RHO-FAMILY-MEMBERS (more than ten distinct proteins eg. RhoA, Rac1, Cdc42) AND MAPK-PATHWAYS (potentially the JNK-cascade). Two regions found in TEM5 give a hint on its function: a central region of 300 aa containing a Dbl homology domain in tandem with a pleckstrin homology domain, and C-terminal beta-propeller forming region with homology to JSAP-1 and mSYD2 (AF262046).

     The DH domain is known to confer catalytic GEF-activity. Family members show great variation in target-specificity: with either a broad GE-specificity (Dbl, Ect2), or a restricted GE-specificity (FGD1-cdc 42 specific). An additional function of DH domains besides the catalytic activity, could be the recruitment of GTPases to targets and locations. Closest homology of studied proteins to the TEM5 DH domain can be found in bcr(AAB60390.1), pbl[Drosophila](AAF50508.1), ect2(NP_031926.1) with identities in the range of 30%.

     The C-terminal beta-propeller forming region shows 27% identity to the C-terminal region in JSAP1 (BAB16676.1) and mSYD2 (AF262046).

  The homologous region in JSAP1 was shown to be involved in direct binding to Raf-1(MAPKKK), and in binding to MEK1(MAPKK) and SEK1(MAPKK) which could be indirect. No function could be reliably proposed for these binding properties, given that the region was not essential for activation of the MEKK1-SEK1 pathway. It has been proposed that JSAP1 might regulate the extent to which the ERK and JNK cascade can be activated by binding the catalytic C-terminus of Raf-1.
  Msyd2 is a neuronal enriched protein that has been shown to acts as a scaffold for the c-Jun N-terminal kinase (JNK) group of MAPK. Msyd2 belongs to the family of syd-related proteins, which includes two classes with similarity in their C-terminus but dissimlar N-terminus. One class is defined by a N-terminal coiled-coil domain. The second by an N-terminal GEF motif.
  
  

     A proline, serine rich LCR, which lies inbetween the DH/PH and the beta-propeller forming region, shows significantly scoring PEST sites in the region 1019 to 1158, the best being 1076 to 1104 (+14.31).

     Due to the potential integrative function of TEM5 in signaling and the fact that the proline-rich regions of Tem5 contain several minimal SH3 binding site (PXXP) the protein associates with Src homology 3-containing signaling proteins. Additionally, an match with the exact binding site for 14-3-3 within the aminoterminal region of Tem5 might indicate a role for 14-3-3 proteins in regulation of the protein activity.

	Sequence Information

TEM	TEM5
Reliability of Gen2Tag mapping	Cid->tag (Hs.45180) frequency: tem5: CATG-TATTAACTCT=31/36 (EST: oriented) tem5: CATG-TATTAACTCT=2/2 (cDNA)
Analysis	BlastP against nr Output Created by a Suite of Single Sequence Analysis Techniques
Protein Description	SEQ.SOURCE: gi|2224615|dbj|BAA20795.1| KIAA0337 [Homo sapiens]
Protein Sequence	MARRLPRTSALKSSSSELLLTGPGAEEDPLPLIVQDQYVQEARQVFEKIQRMGAQQDDGSDAPPGSPDWA GDVTRGQRSQEELSGPESSLTDEGIGADPEPPVAAFCGLGTTGMWRPLSSSSAQTNHHGPGTEDSLGGWA LVSPETPPTPGALRRRRKVPPSGSGGSEFSNGEAGEAYRSLSDPIPQRHRAATSEEPTGFSVDSNLLGSL SPKTGLPATSAMDEGLTSGHSDWSVGSEESKGYQEVIQSIVQGPGTLGRVVDDRIAGKAPKKKSLSDPSR RGELAGPGFEGPGGEPIREVEPMLPPSSSEPILVEQRAEPEEPGATRSRAQSERALPEALPPPATAHRNF HLDPKLADILSPRLIRRGSKKRPARSSHQELRRDEGSQDQTGSLSRARPSSRHVRHASVPATFMPIVVPE PPTSVGPPVAVPEPIGFPTRAHPTLQAPSLEDVTKQYMLNLHSGEVPAPVPVDMPCLPLAAPPSAEAKPP EAARPADEPTPASKCCSKPQVDMRKHVAMTLLDTEQSYVESLRTLMQGYMQPLKQPENSVLCDPSLVDEI FDQIPELLEHHEQFLEQVRHCMQTWHAQQKVGALLVQSFSKDVLVNIYSAYIDNFLNAKDAVRVAKEARP AFLKFLEQSMRENKEKQALSDLMIKPVQRIPRYELLVKDLLKHTPEDHPDHPLLLEAQRNIKQVAERINK GVRSAEEAERHARVLQEIEAHIEGMEDLQAPLRRFLRQEMVIEVKAIGGKKDRSLFLFTDLIVCTTLKRK SGSLRRSSMSLYTAASVIDTASKYKMLWKLPLEDADIIKGASQATNRENIQKAISRLDEDLTTLGQMSKL SESLGFPHQSLDDALRDLSAAMHRDLSEKQALCYALSFPPTKLELCATRPEGTDSYIFEFPHPDARLGFE QAFDEAKRKLASSKSCLDPEFLKAIPIMKTRSGMQFSCAAPTLNSCPEPSPEVWVCNSDGYVGQVCLLSL RAEPDVEACIAVCSARILCIGAVPGLQPRCHREPPPSLRSPPETAPEPAGPELDVEAAADEEAATLAEPG PQPCLHISIAGSGLEMTPGLGEGDPRPELVPFDSDSDDESSPSPSGTLQSQASRSTISSSFGNEETPSSK EATAETTSSEEEQEPGFLPLSGSFGPGGPCGTSPMDGRALRRSSHGSFTRGSLEDLLSVDPEAYQSSVWL GTEDGCVHVYQSSDSIRDRRNSMKLQHAASVTCILYLNNQVFVSLANGELVVYQREAGHFWDPQNFKSVT LGTQGSPITKMVSVGGRLWCGCQNRVLVLSPDTLQLEHMFYVGQDSSRCVACMVDSSLGVWVTLKGSAHV CLYHPDTFEQLAEVDVTPPVHRMLAGSDAIIRQHKAACLRITALLVCEELLWVGTSAGVVLTMPTSPGTV SCPRAPLSPTGLGQGHTGHVRFLAAVQLPDGFNLLCPTPPPPPDTGPEKLPSLEHRDSPWHRGPAPARPK MLVISGGDGYEDFRLSSGGGSSSETVGRDDSTNHLLLWRV
SAGE-Tag	CATG-TATTAACTCTC
EST/cluster Description	UNIGEN CLUSTER: Hs.45180 : KIAA0337 gene product SEQ.SOURCE: gi|2224614|dbj|AB002335.1|AB002335 Human mRNA for KIAA0337 gene MARKINGS: TEM5 Tag in red first Poly-A-signal following the Tag in bold
EST/cluster Sequence	ACGACCTATGGTCTAGTAGGGGTTCTGGGGGCTGGGGCGTGTACCGCTCCCCTAGCTTTGGAGCTGGGGA AGGGCTCCTGCGGTCCCAGGCTCGAACCCGTGCCAAAGGACCTGGAGGCACCTCTAGGGCATTGAGGGAT GGAGGATTTGAGCCTGAAAGAGTCGACAGCGGAAGTCCCTGTCAAATCCAGATATCGCCTCAGAGACCCT GACGCTTCTCAGTTTCCTGCGCTCAGACCTTTCAGAGCTGAGGGTCCGAAAACCTGGTGGGAGCTCCGGG GACCGTGGAAGCAACCCCCTAGATGGCAGAGACTCACCATCCGCAGGTGGCCCTGTGGGGCAACTTGAAC CCATACCCATCCCAGCCCCAGCATCACCTGGCACGCGCCCCACACTCAAGGACTTGACAGCCACTCTGCG GAGAGCAAAGTCATTCACCTGCTCTGAGAAGCCCATGGCCCGCCGCCTGCCCCGCACCAGTGCTCTGAAG TCCAGCTCCTCCGAGCTCCTGCTCACAGGCCCTGGTGCCGAGGAGGATCCGCTGCCCCTCATCGTCCAGG ACCAATATGTGCAGGAGGCCCGCCAGGTTTTTGAGAAGATCCAGCGCATGGGTGCCCAACAAGATGATGG AAGCGATGCCCCCCCTGGAAGCCCTGACTGGGCAGGGGATGTGACCCGAGGGCAGCGGTCCCAGGAGGAG CTCTCAGGCCCTGAGTCCAGTCTGACAGATGAAGGCATTGGGGCAGACCCTGAGCCTCCTGTTGCAGCAT TTTGCGGCCTGGGTACCACAGGGATGTGGCGACCTCTTTCCTCATCCTCGGCCCAGACGAACCACCATGG CCCTGGGACTGAGGACAGTCTGGGCGGGTGGGCCCTGGTGTCGCCTGAGACCCCTCCCACACCAGGTGCC CTCCGCCGACGACGCAAAGTCCCACCTTCAGGTTCTGGTGGGAGCGAATTTAGCAATGGGGAGGCAGGGG AGGCCTACAGGTCCCTGAGTGACCCAATTCCTCAGCGCCACCGGGCTGCCACCTCTGAAGAGCCTACTGG GTTCTCTGTGGACAGCAACCTCCTGGGCTCACTGAGCCCCAAGACAGGGCTCCCTGCCACCTCAGCCATG GATGAGGGCTTGACCAGTGGTCACAGTGACTGGTCTGTGGGCAGTGAAGAGAGCAAGGGATATCAGGAGG TTATTCAGAGCATAGTTCAGGGGCCTGGCACCCTGGGGCGTGTGGTGGACGACAGGATTGCTGGCAAAGC CCCCAAGAAGAAATCCCTGAGTGACCCCAGCCGCCGTGGGGAGCTGGCTGGGCCTGGATTCGAGGGCCCT GGAGGGGAGCCCATCCGAGAAGTTGAGCCCATGCTGCCTCCATCCAGCAGCGAGCCCATCCTTGTAGAGC AGCGGGCAGAGCCAGAAGAACCTGGTGCCACCAGGAGCCGGGCACAGTCTGAAAGGGCCCTACCTGAGGC TCTGCCTCCCCCTGCCACTGCCCACCGAAACTTTCACCTTGACCCCAAGCTGGCTGACATTCTGTCCCCG AGGCTAATCCGCCGAGGCTCCAAGAAGCGCCCAGCTCGGAGTAGTCACCAGGAGCTTCGGAGAGACGAGG GCAGTCAGGACCAGACTGGCAGCCTGTCTCGGGCCCGGCCCTCCTCCAGACACGTTCGCCATGCCAGTGT GCCCGCCACATTTATGCCTATTGTGGTGCCTGAGCCACCAACTTCTGTTGGTCCCCCTGTGGCTGTGCCA GAACCCATAGGCTTCCCTACCCGAGCCCATCCCACGTTGCAGGCACCATCGCTCGAGGACGTCACCAAGC AGTACATGCTGAACCTGCACTCCGGTGAGGTCCCTGCCCCAGTGCCAGTGGACATGCCCTGCTTGCCTCT GGCTGCACCGCCCTCTGCTGAGGCCAAGCCCCCTGAGGCAGCTCGGCCTGCAGATGAGCCTACCCCTGCC AGCAAGTGCTGCAGCAAGCCACAGGTGGACATGCGGAAGCACGTGGCCATGACCCTGCTGGACACAGAGC AGTCGTATGTGGAGTCGCTGCGCACCCTGATGCAGGGCTACATGCAGCCGCTGAAGCAGCCAGAGAACTC CGTGCTCTGTGACCCTTCACTGGTGGACGAGATCTTCGACCAGATCCCCGAGCTCCTGGAGCACCACGAG CAATTCCTGGAGCAGGTTCGGCACTGCATGCAGACCTGGCATGCCCAGCAGAAGGTGGGAGCCCTGCTCG TCCAGTCGTTCTCCAAGGATGTCCTAGTAAACATCTATTCTGCCTATATCGATAACTTCCTCAATGCAAA GGATGCTGTGCGTGTGGCCAAGGAGGCGAGGCCTGCCTTTCTCAAGTTCCTAGAGCAAAGCATGCGTGAG AACAAGGAGAAGCAGGCGCTGTCTGACCTCATGATCAAGCCTGTGCAGCGGATCCCACGCTACGAGCTTC TGGTGAAGGACCTCCTGAAGCATACACCTGAGGACCACCCGGACCATCCACTCCTGCTGGAGGCGCAGCG GAACATCAAGCAGGTGGCTGAGCGCATCAACAAGGGTGTGCGGAGTGCCGAGGAGGCGGAGCGCCATGCC CGTGTGCTGCAGGAGATAGAGGCTCACATCGAGGGCATGGAGGATCTCCAGGCCCCTCTGCGGCGGTTCC TGAGACAGGAGATGGTCATTGAAGTGAAGGCGATCGGTGGCAAGAAGGACCGGTCTCTCTTCCTGTTCAC GGACCTCATCGTCTGCACCACTCTGAAGCGAAAGTCAGGCTCCCTGCGGCGCAGCTCCATGAGCCTGTAC ACGGCAGCCAGTGTCATTGACACAGCCAGCAAGTACAAGATGCTGTGGAAGCTGCCGCTGGAAGACGCAG ACATCATCAAAGGGGCATCCCAAGCCACCAATCGGGAGAACATCCAGAAGGCCATCAGCCGCCTTGATGA GGACCTCACCACCCTGGGCCAAATGAGCAAGCTCTCTGAGAGCCTTGGTTTCCCCCACCAGAGCCTGGAC GATGCACTGCGGGACCTCTCAGCTGCCATGCACCGGGACCTGTCGGAGAAGCAGGCGCTGTGCTACGCGC TTTCCTTCCCGCCAACCAAGCTGGAGCTGTGCGCCACTCGGCCCGAGGGCACCGACTCCTACATTTTTGA GTTCCCTCACCCTGACGCCCGCCTTGGTTTTGAACAGGCCTTCGATGAGGCCAAGAGGAAGCTGGCATCC AGCAAAAGCTGTCTAGACCCTGAGTTCCTGAAGGCCATCCCCATCATGAAAACCCGCAGTGGCATGCAGT TCTCCTGTGCGGCTCCCACCCTGAACAGCTGCCCGGAGCCCTCGCCTGAGGTATGGGTCTGCAACAGCGA CGGCTACGTGGGCCAGGTGTGCCTGCTGAGCCTGCGCGCCGAGCCGGACGTGGAGGCCTGCATCGCCGTC TGTTCCGCCCGCATCCTCTGCATCGGGGCGGTGCCCGGGCTGCAGCCTCGCTGCCACCGGGAGCCTCCTC CGTCGCTGAGGAGTCCTCCAGAGACGGCACCGGAGCCCGCCGGGCCGGAGCTGGACGTCGAGGCCGCTGC AGACGAGGAAGCCGCGACGCTCGCGGAGCCGGGGCCGCAGCCCTGCCTTCACATCTCCATTGCAGGCTCG GGCTTGGAGATGACGCCGGGCCTCGGCGAGGGTGACCCCCGCCCAGAGCTGGTGCCCTTTGACAGTGACT CTGACGATGAGTCTTCGCCCAGCCCCTCGGGGACGCTGCAGAGCCAGGCCAGCCGGTCCACCATCTCCTC CAGCTTTGGCAATGAGGAGACCCCGAGTTCCAAGGAGGCCACGGCAGAGACCACCAGCTCAGAGGAGGAG CAGGAGCCAGGCTTCCTGCCACTGTCTGGCTCCTTTGGGCCTGGTGGTCCCTGCGGCACCAGCCCAATGG ATGGGAGAGCCCTTCGCCGCTCCAGCCACGGCTCCTTCACCCGGGGCAGCCTTGAGGACCTGCTGAGTGT CGACCCTGAGGCCTACCAGAGCTCCGTGTGGCTGGGCACTGAGGATGGCTGTGTCCACGTGTACCAGTCC TCCGACAGCATCCGTGACCGCAGGAACAGCATGAAGCTCCAGCATGCGGCCTCTGTGACCTGCATCTTGT ATCTGAATAACCAGGTGTTTGTGTCTCTGGCCAATGGAGAGCTTGTGGTCTACCAAAGGGAAGCAGGCCA TTTCTGGGACCCCCAGAACTTCAAATCAGTGACCTTGGGCACCCAGGGGAGCCCCATCACCAAGATGGTA TCTGTGGGTGGGCGGCTGTGGTGTGGCTGCCAGAACCGAGTCCTTGTCCTGAGCCCTGACACGCTGCAGC TGGAGCACATGTTTTACGTGGGTCAGGATTCAAGCCGCTGCGTGGCTTGCATGGTGGACTCCAGCCTGGG TGTGTGGGTGACATTGAAAGGTAGTGCCCACGTGTGTCTCTACCATCCAGACACCTTTGAGCAGCTGGCA GAAGTAGACGTCACTCCTCCCGTGCACAGGATGCTGGCAGGCTCGGATGCCATCATCCGGCAGCACAAGG CTGCCTGTCTGCGAATCACAGCGCTGCTGGTGTGTGAGGAGCTGCTGTGGGTGGGCACCAGTGCTGGTGT CGTCCTCACCATGCCCACTTCGCCCGGTACTGTCAGCTGCCCACGGGCACCACTCAGTCCCACAGGCCTC GGCCAGGGACACACCGGCCACGTCCGCTTCTTGGCTGCAGTCCAGCTGCCAGATGGCTTCAACCTGCTCT GCCCAACCCCACCACCTCCCCCAGACACAGGCCCCGAGAAGCTGCCATCACTGGAGCACCGGGACTCCCC TTGGCACCGAGGCCCCGCCCCTGCCAGGCCTAAAATGCTGGTTATCAGTGGAGGTGATGGCTATGAGGAC TTCCGACTCAGCAGTGGGGGCGGCAGCAGCAGTGAGACTGTGGGTCGAGACGACAGCACAAACCACCTCC TCCTGTGGAGGGTGTGACCCTGTCTGCCGTGGCCCAGGACTCGCCCGCCCACCTGCCTTCAGCCTGCTTG CCTCTCCCTAGCCCACACGCAGACTTTGACCAGGAGTATCCAGCCAGGGGCACACATGTGCCTGCGTGGG CTCTGCCTTGTCTTCGCGGAAGCATTCCTGATGGAACACCCACTGGCCAGCCAGGCCATGGCTTCTCCCG ACCCTCTGGCTGCCCCGGTGCTTCCAGTCATGATCGGGTGGGGGACATGTGGGCTGACCAGGACCTCTGA CCCTGGAGCTTCTACCAAAGACACAGCTGGGTCTGGACCCCACGGGGCTGGGGAGGGCCATGTGCAATAT TTGGAGGGTTTTCTGGAGGGCAGCAGGAAGGCTGGGGAATTCCCCATGTACAGTATTTATGTTTCTTTTT AGATGTGTACCTTCCCAAGCACTTATTTATGCAGTGACCTGGTCACCTGGGGTGGGGGTGATTTGAGGAA ATGACATGAGGAAAAGAAACCTATTCCTGCCCTGGGGACCACCCTGGGACTCTAACCAAGCCTTCCTGGA GGGACCCATGCGCCCCTGAGCCCCATTCCATTCATACAGACACACACGTACGCACACTGCATGTCCAAGG CCCTAAACATTGCCCGTTGACATAAACTTTCCAGGGCCCCAGCCTGATGGGGCTGCCCTCAGTCCTCTAG ATCAAGATGCTGACTATTAGGGGGCAGTGATTGCCATCTGGGGACCTGTCAGGCTTTGTCATTTCCCAGT TTGTTGGTGGTGCCTTTAGTGGTTCCCTAATTTGGGAACACTGATGGGGCCTTGGACAGGGCTTTCTCTC AGGTAGGAGAAATGGGCCCATGATCTCCTCACAGTCGCCCCCAGTCCTTGGCCCTGCTTCCCTGTGTCTC ATGCACTGGCACATATGGTCACCTTGGAGGGCAGACCTAGGAGCCCCTCTGACCACTGAATCCGTCTCCA CACCCCTTCTGCCAAGGGAAGCCCCTTCAGGAAGGACCCCCCAAAGCTGAGGGGCTGAATGTAGCCTTTT CAACAGAGAAGGCTCCCACTTGAGAGCAGCCTCTACCTGACCCCCTGGACCACAGAGAGCCACTCTGACC CTCAGCCCCCTCGCTTCTTCAGCTAAAACTCCAAAGGTTTGGTTTCAGATGGGGTTTGTTTTGTTCTGTT TGGTTTTGGTTTTGTTTGGGGTGGGTGGGTCATTGCGGTCTTAGATTATGTTTCTCTTGCTACCAAACAG TCATGTATTAACTCTCTTTGGATGATGAAGTTTAAAGAGTCAATAAATAGAAACACCAG
Genomic Sequence Description
Genomic Sequence

	Literature