Tumor Endothelial Marker Report: TEM46 (Peanut-like 1)

TEM domain structure

	TEM domain structure

TEM Sequence Analysis

name from to source/E description

GTP_CDC 41 321 Pfam, E = 1.4e-185 Contains a P-loop nucleotide-binding consensus sequence (SCOP).

369

	TEM Sequence Analysis

name	from	to	source/E	description
GTP_CDC	41	321	Pfam, E = 1.4e-185	Contains a P-loop nucleotide-binding consensus sequence (SCOP).
369

Remarks

	Remarks

TRANSCRIPTION VARIANTS: Two known transcripts of the gene exist: a major one of 2.2 kb and a minor one of 3.5 kb. The 2.2 kb transcript form which encodes the 369 amino acid protein results from the utilization of a non-consensus polyA_signal (AACAAT) within this gene itself. In the absence of polyadenylation from its own imperfect site, this gene uses the consensus polyA_signal within its downstream neighbour gene (GP1BB; platelet glycoprotein Ib), resulting in the 3.5 kb read-through transcript. A transcript variant with a different 5 end arising by alternative splicing, and possibly accompanied by alternative promoter usage, has also been identified, however, its full length nature is not known.
FUNCTION: The septins are a family of GTPase enzymes that assemble as intracellular filamentous scaffolds involved in the organization of submembranous structures, neuronal polarity, and vesicle trafficking. The brain-specific human CDCrel-1 has been shown to be essential for exocytosis in neurons. It associates with synaptic vesicles, binds other septins and syntaxin. It is hypothesized that CDCrel-1 can have both a positive and a negative role in exocytosis.

Sequence Information

TEM TEM46

Reliability of Gen2Tag mapping

Cid->tag (Hs.3847) frequency:
tem46: CATG-ATCCCTTCCCG=5/6 (cDNA)
tem46: CATG-ATCCCTTCCCG=12/12 (EST oriented)

Analysis
BlastP against nr

Output Created by a Suite of Single Sequence Analysis Techniques

Protein Description

SEQ.SOURCE: sp|Q99719|PNL1_HUMAN PEANUT-LIKE PROTEIN 1

Protein Sequence

MSTGLRYKSKLATPEDKQDIDKQYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKSTLVHSLFLTDL YKDRKLLSAEERISQTVEILKHTVDIEEKGVKLKLTIVDTPGFGDAVNNTECWKPITDYVDQQFEQYFR DESGLNRKNIQDNRVHCCLYFISPFGHGLRPVDVGFMKALHEKVNIVPLIAKADCLVPSEIRKLKERIR EEIDKFGIHVYQFPECDSDEDEDFKQQDRELKESAPFAVIGSNTVVEAKGQRVRGRLYPWGIVEVENQA HCDFVKLRNMLIRTHMHDLKDVTCDVHYENYRAHCIQQMTSKLTQDSRMESPIPILPLPTPDAETEKLI RMKDEELRRMQEMLQRMKQQMQDQ

SAGE-Tag CATG-ATCCCTTCCCG

EST/cluster Description

UNIGEN CLUSTER: Hs.3847 : peanut (Drosophila)-like 1

SEQ.SOURCE: gi|9945438|ref|NM_002688.2| Homo peanut (Drosophila)-like 1 (PNUTL1)

REMARKS: Known transcripts of the gene: a major one of 2.2 kb and a minor one of 3.5 kb.

EST/cluster Sequence

CACCCCGCAGCCCGGCCTCGGCCTCCGCCGCTTGTTGTCGCGCCCCGCCCGCGAGCCCGCCCCGCACGTC CCCCGCCGGCGGCCACCATGAGCACAGGCCTGCGGTACAAGAGCAAGCTGGCGACCCCAGAGGACAAGCA GGACATTGACAAGCAGTACGTGGGCTTCGCCACACTGCCCAACCAGGTGCACCGCAAGTCGGTGAAGAAA GGCTTTGACTTCACACTCATGGTGGCTGGTGAGTCAGGCCTGGGGAAGTCCACACTGGTCCACAGCCTCT TCCTGACAGACTTGTACAAGGACCGGAAGCTGCTCAGTGCTGAGGAGCGCATCAGCCAGACGGTAGAGAT TCTAAAACACACGGTGGACATTGAGGAGAAGGGAGTCAAGCTGAAGCTCACCATCGTGGACACGCCGGGA TTCGGGGACGCTGTCAACAACACCGAGTGCTGGAAGCCCATCACCGACTATGTGGACCAGCAGTTTGAGC AGTACTTCCGTGATGAGAGCGGCCTCAACCGAAAGAACATCCAAGACAACCGAGTGCACTGCTGCCTATA CTTCATCTCCCCCTTCGGGCATGGGCTGCGGCCAGTGGATGTGGGTTTCATGAAGGCATTGCATGAGAAG GTCAACATCGTGCCTCTCATCGCCAAAGCTGACTGTCTTGTCCCCAGTGAGATCCGGAAGCTGAAGGAGC GGATCCGGGAGGAGATTGACAAGTTTGGGATCCATGTATACCAGTTCCCTGAGTGTGACTCGGACGAGGA TGAGGACTTCAAGCAGCAGGACCGGGAACTGAAGGAGAGCGCGCCCTTCGCCGTTATAGGCAGCAACACG GTGGTGGAGGCCAAGGGGCAGCGGGTCCGGGGCCGACTGTACCCCTGGGGGATCGTGGAGGTGGAGAACC AGGCGCATTGCGACTTCGTGAAGCTGCGCAACATGCTCATCCGCACGCATATGCACGACCTCAAGGACGT GACGTGCGACGTGCACTACGAGAACTACCGCGCGCACTGCATCCAGCAGATGACCAGCAAACTGACCCAG GACAGCCGCATGGAGAGCCCCATCCCGATCCTGCCGCTGCCCACCCCGGACGCCGAGACTGAGAAGCTTA TCAGGATGAAGGATGAGGAACTGAGGCGCATGCAGGAGATGCTGCAGAGGATGAAGCAGCAGATGCAGGA CCAGTGACGCTCGCCGCGGACACACCGTCCGTCTCCGGGACGCCCTCGCACCCCTGGACACCAGACCGGA CTGTTCCCGACCCGGAGACGCGGGGCCACAGCCCCCAGCTGACCCTAATTTATTCTCAGCACCACCCCCT CCCAGGTCATTGTGTCTGTTTCCGAGGGGCCTGGACCGTAGCCCCCGCCCAGCTGGCCCTCTCTGACCTT GGGGGATCAGGAGCGAAGTTGGGCGGGACTTCAGAGATCCGCCTCCCTTGCCCTTCCCCCGCCCCCGGAC GGTCACAGCACCCAAACCGCAGGCCCTGCTCTGGCAGGCAGGCAAAGCTAGGCAGAAGAGGATTCCCAGG ATCCTGGGTCTGTTCCCTGCCCCAGTGCTGCAGAACGGACTTGGGAGCCCTCCTTTGCCTGCTCCCGCGG GTCACCCAGCGAGTGCTGAGACCCCATTTTCTGTCGAGGCGGGCCGAGTCTTCCCTTATCCCCAGACGCC TAGCGGGCAGGGTTGGGCTGAATCAAATGGGAGCCCTCCAGACATAAGGAGGCCAGAGGCTGCAAGGAGC GGGGTCGTGACCGCTTACACCCCTTCTCCACAGCCCGGCCCGACCTGGAGGGCCCCCGGGGCACTGGGCG GTGAGCCACCTCCTGGCAACTCTCGGTGCCGTCCCCTGCCCTCGCTCGAGGCCTCTTCTCCCCAGCACCG CTGTGGTGTGCCGGGATCCTGAGCCTAGGCCTCCCGATGTTCCCACCCGCATGATCCCTTCCCGCCACAC GATGCTCCGTTTTCTTCCGTTGTGAATGCCGCGTCCTGTCCTGGTGACAGGAGAACAATGTTGGTGAACG TCAAAAAAAAAAAAAAAA

Genomic Sequence Description

Genomic Sequence

	Sequence Information

Literature

	Literature

PubMed: 9022087 Alternative expression of platelet glycoprotein Ib(beta) mRNA from an adjacent 5 gene with an imperfect polyadenylation signal sequence.
Zieger B, Hashimoto Y, Ware J.
J Clin Invest 1997 Feb 1;99(3):520-5.

PubMed: 10321247 The septin CDCrel-1 binds syntaxin and inhibits exocytosis.
Beites CL, Xie H, Bowser R, Trimble WS.
Nat Neurosci. 1999 May;2(5):434-9.

PubMed: 9611266 Structure and expression of the human septin gene HCDCREL-1.
Yagi M, Zieger B, Roth GJ, Ware J.
Gene 1998 Jun 8;212(2):229-36.

FAQ

PubMed: 9022087	Alternative expression of platelet glycoprotein Ib(beta) mRNA from an adjacent 5 gene with an imperfect polyadenylation signal sequence. Zieger B, Hashimoto Y, Ware J. J Clin Invest 1997 Feb 1;99(3):520-5.
PubMed: 10321247	The septin CDCrel-1 binds syntaxin and inhibits exocytosis. Beites CL, Xie H, Bowser R, Trimble WS. Nat Neurosci. 1999 May;2(5):434-9.
PubMed: 9611266	Structure and expression of the human septin gene HCDCREL-1. Yagi M, Zieger B, Roth GJ, Ware J. Gene 1998 Jun 8;212(2):229-36.