Tumor Endothelial Marker Report: TEM45 (no convincing assignment: tropomyosin 1)

	TEM domain structure

	TEM Sequence Analysis

name	from	to	source/E	description
Tropomyosin	48	284	Pfam, E = 1e-46	Tropomyosin is an alpha-helical protein that forms a coiled-coil structure of 2 parallel helices containing 2 sets of 7 alternating actin binding sites
284

	Remarks

• RELIABILITY of the tag assignment:
  The tag is questionable for several reasons:
  • the tag is invers, although close to a polyT run.
  • the tag with highest reliability (TTAAAGATTT) cannot be found in either TEMs nor in PEMs in the region and, thus, an upregulation of the transcript cannot be supported.
    
  • note: the tag-encluding region has been sequenced wrongly in the cDNA.
    aaaaggcaagcccatg in the EST (correct)
    aaaag-caagcccatg in the CDS
    

• DOMAIN ARCHITECTURE: The muscle protein tropomyosin consists of two parallel alpha-helical tropomyosin molecules that are wound around each other forming a coiled-coil dimer.

• FUNCTION: Tropomyosin (TM) is a major actin-binding proteins that can modulate the stability of actin filaments. The mode of interaction with actin filaments is dependent on the actin and tropomyosin isoform. The TM binding can occur on the inner or the outer domain of actin. Tropomyosin is a coiled-coil that binds head-to-tail along the helical actin filament. Tropomyosin has a role in steric hindrance to the binding of myosin to actin and in stabilization of actin filament structure.
  Tropomyosin (TM) is known to be essential for the reorganization of the actin cytoskeleton mediated by stabilization of microfilaments.

	Sequence Information

TEM	TEM45
Reliability of Gen2Tag mapping	Cid->tag (Hs.77899) frequency: tem45: CATG-GGCTTGCCT=6/29 (EST oriented, 5labeled)      : CATG-TTAAAGATT=45/72 (EST unoriented, 5labeled) tem45: CATG-GGCTTGCCT=2/184 (EST oriented)      : CATG-TTAAAGATT=143/184 (EST oriented)
Analysis	Output Created by a Suite of Single Sequence Analysis Techniques
Protein Description	SEQ.SOURCE: gi|339944|gb|AAA36771.1| tropomyosin
Protein Sequence	MDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQEK LELAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEK MEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGQVRQLEEQLRIMDQTLKALMAA EDKYSQKEDRYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEEKVAHAKEENLSMHQMLDQTLLE LNNM
SAGE-Tag	CATG-GGCTTGCCTTT
EST/cluster Description	UNIGEN CLUSTER: Hs.77899 : tropomyosin 1 (alpha) SEQ.SOURCE: gb|M19267.1|HUMTRO Human tropomyosin mRNA MARKINGS: tem45 Tag in red first Poly-A-signal following the Tag in bold REMARKS: The sequence of the tag is incorrect in the published mRNA Proof: BlastN of 1261 to 1330 against human EST
EST/cluster Sequence	CAGAATCTCCGGCAGTTTTTGTACCTCAAGAAGTAAGTGGAACACCTTTCCCTGTCATAGTTATTTTCAT CCAGACATCTGGTGGAAGCATCAGATTCCTTACAGATATAAGAGAGGCATCATTTAAAAGGTAGAACAGG ATCGACAAACAAGGATTTATGTCAGGATCTCTCAGCCTCTGTGTTACCGAGGGCATTTCTAACAGTCTTC TTACTACGGCCTCCGCCGACCGCGCGCTCGCCCCGCCGCTCCTGCTGCAGCCCCAGGGCCCCTCGCCGCC GCCACCATGGACGCCATCAAGAAGAAGATGCAGATGCTGAAGCTCGACAAGGAGAACGCCTTGGATCGAG CTGAGCAGGCGGAGGCCGACAAGAAGGCGGCGGAAGACAGGAGCAAGCAGCTGGAAGATGAGCTGGTGTC ACTGCAAAAGAAACTCAAGGGCACCGAAGATGAACTGGACAAATACTCTGAGGCTCTCAAAGATGCCCAG GAGAAGCTGGAGCTGGCAGAGAAAAAGGCCACCGATGCTGAAGCCGACGTAGCTTCTCTGAACAGACGCA TCCAGCTGGTTGAGGAAGAGTTGGATCGTGCCCAGGAGCGTCTGGCAACAGCTTTGCAGAAGCTGGAGGA AGCTGAGAAGGCAGCAGATGAGAGTGAGAGAGGCATGAAAGTCATTGAGAGTCGAGCCCAAAAAGATGAA GAAAAAATGGAAATTCAGGAGATCCAACTGAAAGAGGCAAAGCACATTGCTGAAGATGCCGACCGCAAAT ATGAAGAGGTGGCCCGTAAGCTGGTCATCATTGAGAGCGACCTGGAACGTGCAGAGGAGCGGGCTGAGCT CTCAGAAGGCCAAGTCCGACAGCTGGAAGAACAATTAAGAATAATGGATCAGACCTTGAAAGCATTAATG GCTGCAGAGGATAAGTACTCGCAGAAGGAAGACAGATATGAGGAAGAGATCAAGGTCCTTTCCGACAAGC TGAAGGAGGCTGAGACTCGGGCTGAGTTTGCGGAGAGGTCAGTAACTAAATTGGAGAAAAGCATTGATGA CTTAGAAGAGAAAGTGGCTCATGCCAAAGAAGAAAACCTTAGTATGCATCAGATGCTGGATCAGACTTTA CTGGAGTTAAACAACATGTGAAAACCTCCTTAGCTGCGACCACATTCTTTCATTTTGTTTTGTTTTGTTT TGTTTTTAAACACCTGCTTACCCCTTAAATGCAATTTATTTACTTTTACCACTGTCACAGAAACATCCAC AAGATACCAGCTAGGTCAGGGGGTGGGGAAAACACATACAAAAAGCAAGCCCATGTCAGGGCGATCCTGG TTCAAATGTGCCATTTCCCGGGTTGATGCTGCCACACTTTGTAGAGAGTTTAGCAACACAGTGTGCTTAG TCAGCGTAGGAATCCTCACTAAAGCAGGAGAAGTTCCATTCAAAGTGCCAATGATAGAGTCAACAAGGAA GGTTAATGTTGGAAACACAATCAGGTGTGGATTGGTGCTACTTTGAACAAAAGGTCCCCCTGTGGTCTTT TGTTCAACATTGTACAATGTAGAACTCTGTCCAACACTAATTTATTTTGTCTTGAGTTTTACTACAAGAT GAGACTATGGATCCCGCATGCCT
Genomic Sequence Description
Genomic Sequence

	Literature

PubMed: 7943254

Hypoxia induces the synthesis of tropomyosin in cultured porcine pulmonary artery endothelial cells. Rao UJ, Denslow ND, Block ER Am J Physiol 1994 Sep;267(3 Pt 1):L271-81