Home

Initium

Angiogenesis

List of -TEMs -PEMs -NEMs

TEMs TEM1 TEM2 TEM3 TEM4 TEM5 TEM6 TEM7 TEM8 TEM9 TEM10 TEM11 TEM12 TEM13 TEM14 TEM15 TEM16 TEM17 TEM18 TEM19 TEM20 TEM21_1 TEM21_2 TEM22 TEM23 TEM24 TEM25 TEM26 TEM27 TEM28 TEM29 TEM30 TEM31_1 TEM31_2 TEM32 TEM33 TEM34 TEM35 TEM36 TEM37 TEM38 TEM39 TEM40 TEM41 TEM42 TEM43 TEM44 TEM45 TEM46

Summary -Overview of TEMs -extracellular TEMs -intrac. & membrane TEMs -TEM tissue distribution

Supplementary -SAGE -Seq.analysis Methods

FAQ

Tumor Endothelial Marker Report: TEM35 (connexin45)

	TEM domain structure

	TEM Sequence Analysis

name	from	to	source/E	description
connexin	1	253	Pfam, E= 1.7e-149	All cloned connexins share a common transmembrane (TM) topology: containing 4 TM domains, with two extracellular and three cytoplasmic regions. View the Pfam connexin alignment to obtain a overview on conserved residues and regions.
cytoplasmic	1	18		Localization was assigned by similarity to other connexins.
TM	20	40	toppred2, 1.180	The TM region fits the conserved connexin topology.
extracellular	40	76	by similarity	Conserved cysteine residue are likely to form intramolecular disulphide bonds, which are supposedly involved in interaction between connexons.
TM	76	96	toppred2, 1.653 by similarity	The TM region fits the conserved connexin topology.
intracellular	96	185	by similarity	The sequence of this loop is highly variable among connexin family members.
TM	185	205	toppred2, 0.952 by similarity	The fourth TM region has an amphipathic character, fits the conserved connexin topology, and is suggested to contribute to the lining of the formed-channel.
extracellular	205	228	by similarity	Conserved cysteine residue are likely to form intramolecular disulphide bonds, which are supposedly involved in interaction between connexons.
TM	228	248	toppred2, 1.946 by similarity	The TM region fits the conserved connexin topology.
intracellular	248	396	by similarity	The sequence of the cytoplasmic C-terminus is highly variable among connexin family members. It is unusually long in Connexin45. A tyrosine phosphorylation site is found at residue 267 and might be an indication of modulation of channel properties through (de)phosphorylation by protein kinases/phosphatases.
coil	327	396		Potentially involved in protein-protein interaction.
396

	Remarks

• RELIABILITY: The Unigen contig Hs.43761 has not been linked previously to the Cx45 gene. Here, the assignment was made following the listed steps:
  • The TEM35-tag was reliably linked to a single Unigen cluster (Hs.43761).
  • This Unigen cluster was oriented relative to a known genomic sequence.
  • The genomic sequence upstream of the TEM35-Unigen cluster was analyzed further. The known gene Cx45 was the first region free of repetitive sequence that could be found upstream of the Unigen cluster Hs.43761. This led to the conclusion that the TEM35-Unigen cluster is linked to the CDS of Cx45.
  View the Blastn against Human Unigen Cluster and the Blastn against Human EstDB using the genomic sequence 7kb upstream of the TEM35 tag that argue for a correct tag to gene assignment.

• CLASSIFICATION: Compared to alpha and beta group connexins, Cx45 has an unusually long cytoplasmic loop. Its amino acid sequence is so divergent from other connexins that it is classified into another subgroup (Goodenough et al, 1996).

• TISSSUE-DISTRIBUTION: Connexin45 (Cx45) has been reported in skin, brain, lung, heart, gut, uterus, ovary and bone (Kirchhoff et al, 1998; Simon et al, 1998).

• FUNCTION:
  GENERAL: Connexins build tightly packed aggregations directly connnecting the cytoplasma of adjacent cells and providing a mechanism for coordinating the activities of groups of cells through the exchange of cytosolic molecules such as ions, second messengers and small metabolites. Inter-endothelial cell contacts are known to be actively opened and closed during angiogenesis.
  SPECIFIC: It also appeared that the Cx45 molecule, like Cx40 and Cx43, contains multiple potential phosphorylation sites which allows for modulation of channel properties through (de)phosphorylation by protein kinases/phosphatases (tyrosine phosphorylation site 267, for a complete list of sites use the sequence analysis output). It has been shown that Cx45 can be phosphorylated at serine residues but also on threonine and tyrosine residues. Cx45 contains putative sites for phosphorylation by PKA.

• DOMAIN ARCHITECTURE: Connexins are thought to contain 4 TM domains, with two extracellular and three cytoplasmic regions. N- and C-termini face the cytoplasm, and the third TM domain has an amphipathic character, suggesting that it contributes to the lining of the formed-channel. Both extracellular loops contain characteristically conserved cysteine residues, which likely form intramolecular disulphide bonds. By contrast, the single putative intracellular loop (between TM domains 2 and 3) and the cytoplasmic C-terminus are highly variable among the family members. Six connexins are thought to associate to form a hemi-channel, or connexon. Two connexons then interact (likely via the extracellular loops of their connexins) to form the complete gap junction channel.

	Sequence Information

TEM	TEM35
Reliability of Gen2Tag mapping	Cid->tag (Hs.43761) frequency: tem35: CATG-GTGGAGACGGA=7/9 (EST, oriented)
Analysis	BlastP against nr Output Created by a Suite of Single Sequence Analysis Techniques
Protein Description	SEQ.SOURCE: sp|P36383|CXA6_HUMAN GAP JUNCTION ALPHA-6 PROTEIN
Protein Sequence	MSWSFLTRLLEEIHNHSTFVGKIWLTVLIVFRIVLTAVGGESIYYDEQSKFVCNTEQPGCENVCYDAFAP LSHVRFWVFQIILVATPSVMYLGYAIHKIAKMEHGEADKKAARSKPYAMRWKQHRALEETEEDNEEDPMM YPEMELESDKENKEQSQPKPKHDGRRRIREDGLMKIYVLQLLARTVFEVGFLIGQYFLYGFQVHPFYVCS RLPCPHKIDCFISRPTEKTIFLLIMYGVTGLCLLLNIWEMLHLGFGTIRDSLNSKRRELEDPGAYNYPFT WNTPSAPPGYNIAVKPDQIQYTELSNAKIAYKQNKANTAQEQQYGSHEENLPADLEALQREIRMAQERLD LAVQAYSHQNNPHGPREKKAKVGSKAGSNKSTASSKSGDGKNSVWI
SAGE-Tag	CATG-GTGGAGACGGA
EST/cluster Description	SEQ.SOURCE: gi|4885168|ref|NM_005497.1| Homo connexin 45, GJA7 ugc|Hs.43761.1|Hs.43761 GENE= PROTSIM= ESTs
EST/cluster Sequence	gi|4885168|ref|NM_005497.1|connexin 45 ATGAGTTGGAGCTTTCTGACTCGCCTGCTAGAGGAGATTCACAACCATTCCACATTTGTGGGGAAGATCT GGCTCACTGTTCTGATTGTCTTCCGGATCGTCCTTACAGCTGTAGGAGGAGAATCCATCTATTACGATGA GCAAAGCAAATTTGTGTGCAACACAGAACAGCCGGGCTGTGAGAATGTCTGTTATGATGCGTTTGCACCT CTCTCCCATGTACGCTTCTGGGTGTTCCAGATCATCCTGGTGGCAACTCCCTCTGTGATGTACCTGGGCT ATGCTATCCACAAGATTGCCAAAATGGAGCACGGTGAAGCAGACAAGAAGGCAGCTCGGAGCAAGCCCTA TGCAATGCGCTGGAAACAACACCGGGCTCTGGAAGAAACGGAGGAGGACAACGAAGAGGATCCTATGATG TATCCAGAGATGGAGTTAGAAAGTGATAAGGAAAATAAAGAGCAGAGCCAACCCAAACCTAAGCATGATG GCCGACGACGGATTCGGGAAGATGGGCTCATGAAAATCTATGTGCTGCAGTTGCTGGCAAGGACCGTGTT TGAGGTGGGTTTTCTGATAGGGCAGTATTTTCTGTATGGCTTCCAAGTCCACCCGTTTTATGTGTGCAGC AGACTTCCTTGTCCTCATAAGATAGACTGCTTTATTTCTAGACCCACTGAAAAGACCATCTTCCTTCTGA TAATGTATGGTGTTACAGGCCTTTGCCTCTTGCTTAACATTTGGGAGATGCTTCATTTAGGGTTTGGGAC CATTCGAGACTCACTAAACAGTAAAAGGAGGGAACTTGAGGATCCGGGTGCTTATAATTATCCTTTCACT TGGAATACACCATCTGCTCCCCCTGGCTATAACATTGCTGTCAAACCAGATCAAATCCAGTACACCGAAC TGTCCAATGCTAAGATCGCCTACAAGCAAAACAAGGCCAACACAGCCCAGGAACAGCAGTATGGCAGCCA TGAGGAGAACCTCCCAGCTGACCTGGAGGCTCTGCAGCGGGAGATCAGGATGGCTCAGGAACGCTTGGAT CTGGCAGTTCAGGCCTACAGTCACCAAAACAACCCTCATGGTCCCCGGGAGAAGAAGGCCAAAGTGGGGT CCAAAGCTGGGTCCAACAAAAGCACTGCCAGTAGCAAATCAGGGGATGGGAAGAACTCTGTCTGGATTTA A ugc|Hs.43761.1|Hs.43761 GENE= PROTSIM= ESTs GAATCCCTGAGCCGGGAGGCGGAGGTTGCAGTGAGCTGAGATTGCACCACTGCACTCCAGCTTGGGTGAT GGAGTGAGACTCAAATAAAAAAGGTACTAGTTCTGCATTTCAGAGTTGGCTTGTTGAACCAGGCTATATG CTTCCAAGATTTAAATGTTTTTCTGTATTATACTCTCAATTGTGTTTTAAAAAAATCTCTTACAGAAATC TCTACCTCAGGCACTAAGTGTTATGACATGGGTAGCATATTGATATTGAAAACTTAGCTAGGACTTCCAG CCTTTTAAGATAATTTAAATGTAAAATTAAATGGTTAACCAGCAATCTAATGTCATGTGGTGTGCAGTTT GGATATTGCATGAACAGCTAAGGAATCACCTGTTCTAGTGCCAAAGATCACTCATTGCTAATTTTGTTCT GTACAGCTTATGTAATATTTTCATGGTGGAGACGGACTCTGTGTGCTCAGGGCCTTGTCTCTAGGAAGAT TTTGTCAATTCCAAATACAGTTTTGAAGATACAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Genomic Sequence Description	SEQ.SOURCE: gi|11128433|gb|AC005180.2|AC005180 Homo sapiens chromosome 17, ANALYSIS: Blastn against Human Unigen Cluster Blastn against Human EstDB MARKINGS: The mRNA sequence (NM_005497.1) is marked in bold.
Genomic Sequence	1 ACCAATTCAATCACCATGAGTTGGAGCTTCCTGACTCGCCTGCTAGAGGAGATTCACAAC 61 CATTCCACATTTGTGGGGAAGATCTGGCTCACTGTTCTGATTGTCTTCCGGATCGTCCTT 121 ACAGCTGTAGGAGGAGAATCCATCTATTACGATGAGCAAAGCAAATTTGTGTGCAACACA 181 GAACAGCCGGGCTGTGAGAATGTCTGTTATGATGCGTTTGCACCTCTCTCCCATGTACGC 241 TTCTGGGTGTTCCAGATCATCCTGGTGGCAACTCCCTCTGTGATGTACCTGGGCTATGCT 301 ATCCACAAGATTGCCAAAATGGAGCACGGTGAAGCAGACAAGAAGGCAGCTCGGAGCAAG 361 CCCTATGCAATGCGCTGGAAACAACACCGGGCTCTGGAAGAAACGGAGGAGGACAACGAA 421 GAGGATCCTATGATGTATCCAGAGATGGAGTTAGAAAGTGATAAGGAAAATAAAGAGCAG 481 AGCCAACCCAAACCTAAGCATGATGGCCGACGACGGATTCGGGAAGATGGGCTCATGAAA 541 ATCTATGTGCTGCAGTTGCTGGCAAGGACCGTGTTTGAGGTGGGTTTTCTGATAGGGCAG 601 TATTTTCTGTATGGCTTCCAAGTCCACCCGTTTTATGTGTGCAGCAGACTTCCTTGTCCT 661 CATAAGATAGACTGCTTTATTTCTAGACCCACTGAAAAGACCATCTTCCTTCTGATAATG 721 TATGGTGTTACAGGCCTTTGCCTCTTGCTTAACATTTGGGAGATGCTTCATTTAGGGTTT 781 GGGACCATTCGAGACTCACTAAACAGTAAAAGGAGGGAACTTGAGGATCCGGGTGCTTAT 841 AATTATCCTTTCACTTGGAATACACCATCTGCTCCCCCTGGCTATAACATTGCTGTCAAA 901 CCAGATCAAATCCAGTACACCGAACTGTCCAATGCTAAGATCGCCTACAAGCAAAACAAG 961 GCCAACACAGCCCAGGAACAGCAGTATGGCAGCCATGAGGAGAACCTCCCAGCTGACCTG 1021 GAGGCTCTGCAGCGGGAGATCAGGATGGCTCAGGAACGCTTGGATCTGGCAGTTCAGGCC 1081 TACAGTCACCAAAACAACCCTCATGGTCCCCGGGAGAAGAAGGCCAAAGTGGGGTCCAAA 1141 GCTGGGTCCAACAAAAGCACTGCCAGTAGCAAATCAGGGGATGGGAAGACCTCCGTCTGG 1201 ATTTAATCCTGGCGGGCTTAAAACCTGTGCTTTTCATAGTTTATGGTAAGCAGCAGCTCA 1261 CTGAATAATGACTTCCATTGAGTAAACATTTGGCTCTGGTTATCTTCAGGGATGCTGTTG 1321 GCTCATGATCCAAGCTCAGGGGACTCTGGAGGCGGGGCTGGGCTGAGGGAGAGAAAGGGA 1381 AACACAGTGTTCCCAGGCACATGTTCTTAGCAATAATACAGTTGCAGAACTTTACATTTG 1441 TGTCTTCCAGATCTGGAGAAGAACAGACATATTTAAATCATTCTTGTTGAACAGTTTTTG 1501 TATGTACAGTATTATGGTACTTTTTTTTTTTTAGTATGAGAACTTTTTTTGTATTTGTAC 1561 ATTGAACTGCTGTAGTTATACTTTTATATTAAAGGGGAAAAAGTCCTTATAAATAATGCC 1621 TATTAGGCTAGTGCTATTACTTTGTTCAGCAAATTCTACCCTGGCTTTAGAGTTTTTAAT 1681 AGATGCTACACCTAATAAAAGTGCCTCTCATATTGCAGGAACGATTTCAGATGTGTAAGG 1741 AGACTGAAGAGGAGATACCAGTTTTTAGTCTTGGAAAGCAAGTTCACAGTAGAAGGAGGT 1801 TGAGATCTTTCTTTTATGTGAGAAATCTTTGAATCTCATTCATGCGATCAGAGTTGTAGC 1861 CAATTTTTGAAAACCTTATTTTCAAAGGAAATAAATGATTCACTGTAGGATTCCTTTAAA 1921 TATCAAGCATCACCAGTATATGCTTTGATGGTATATGTATATAACTTAAAGTTCTTTCAA 1981 AAGCCTGATACAGAAACGTGTCCCCAGTTTGGTAGCAATGTGGAAAACCTGGCTAGAGAT 2041 GATATGGAGCTGTCCCTCAGAAAGCAAAGCCATGCCTGGAATCCCTAATAGGCTGCTTAG 2101 TTGTGAACCTGTTTGATTTGCCTTAAGCCTCTATCCAGAAACCTGCCCGCTTCCGTCTGG 2161 TTAAGAAGCCAGTGGTGGATATTTTCTTTGTTAACATTAGAAATGCAAACATTCCCTTGT 2221 CAACCAAGAATACTCAAAGCTACTTGTATTGGAAATGGCAGAAGGCCTAAATCCAAATTT 2281 CTTATTTTTTATAATTTACCATAGAAGTTTTGTGATTAAATTCTTACTTCTGCCAGTGGA 2341 GGTTTATGCCTGAAAGGTCATGGGGTCCTGTCTGTAAATAGACCTAAAGAGAAGTGCAGT 2401 ATTTATTCTTTGTAGGCATAATGTGTTTGTCACTGACAAGCATTCATGTTCATCCCACTA 2461 GTCTTTTATTGCAGTCTTTTATTGTCATTTTCAGCCTTATGTTGGAGAGCTTTGCTTTCT 2521 CATCATGTTCACATTGTCTTAAGTTTTGTGAGCTTCTGAGAAAGAGCTTGGTAAAGGTTT 2581 AAAGGGGACTTTGTTCCACCAGGGAGCATTTTATTTGGGCGTCTCACCCTTTTCTAATGA 2641 AAGCTGTTGTAAGCCACCTCTGACTTGGAAATTCTGAAAGTATGAATATTTTTTATATCT 2701 TAATTGTAAAATGCCAGTTCTCCATTATTTAGATGAATAGTAGAACACTGCACCCTTTGT 2761 GCAGTGTTTTTGTTTCTCTACTGCATTCCTACCCCCACCAAAAAAAAGAAACTTAAGGAA 2821 ATTTTTTTTTGAGACAGGGTCTCCCTCTTTCACCCAGGCTGGAGTGCAGCAGCATGATCT 2881 CAGCTCACTGCAGCCTCTGCCTCCCAGGCTCAAGCAATCATTCCACCTCAGCCTCTTGAT 2941 TAGCTGGGACTACAGGCATGTGCCACCATACTCGGCTAATTCTGTTTTAAAAATTTTTTT 3001 TGTAGAGATGAGGTCTCACTATGTTACCCAGGCTGGTCTCGAACTTTTGGGTTCAAGTGA 3061 TCCTCCCGCCTCAGCCTCTCAAAGTGTTAGTATTATAGGTGTGAGCCACCATGCCCAGCC 3121 TAGAAAATTTAACCTTATCTTTTAGCCATTCATTTACTTTTATTTGCTTTTTTAAAAAAA 3181 AATCTTTAAATTTTAGCCTAGAGAAGCACCTCAGGAACTCTAAACATCTTAGGCAAAAAT 3241 TAAACTCAGGTGAGACTGAAGGAGAAATTTCAGAAATACACATGTACCTCATGGGTAATT 3301 AATAGACATGCACAGTTTCCTTCAAATCAAATGTTTAATAAGGAAATTCATATAATTCCA 3361 AGAAAACAGGCAAAATTTGGAATACATAAGCCTAGCTAAGATAAAGAACTACCAGTTAAT 3421 TCTTAACATCACTGGAGCTTCCAGCAGGTTTTTTAATTGAAGGACAAAAAGTGTCCATAC 3481 ATTTGTGTTATCTCTTCAGAGCCTGAGGGAGAAGAGAGTGGCTTTGCCCAGTGGAAAGGC 3541 TGGGGATTTGAGAATGATACAGGGGGAAGGAGTCAAGCAGAAATGCCAGGAAGAACCAGG 3601 CAAAGACATTTGGCGCCAGAAGCATCCACTTAATTTCTGTGAAGAGTGCCCCTGGTGTTT 3661 CATCTTGGCCTGTTTTGATGAGAATGTTATCTTTTGTGTCTGGATAACGCGTCAGCTTCT 3721 TAAAGTACATATAAAGATATTCTGTCACCTCCCCACATGCACACACTTTTAAAATCTATT 3781 TTTATTCTCTTGCTAAAGTTGTAATTATGTCAAGAATTTTCCAGCTCTAACTGCCTTCTT 3841 AGTACATGTCTTTCTGCCTTTGAAGCATATGAGTTTGCCAAAGTCATTCTCCCCTAATGA 3901 CATATTGTGGACTTACATTAAGAAAATGGGCCAGGCGCGGTGGCTCACACCTGTAATCCC 3961 AGCACTTTAGGAGGCCCAGGCGGGTGGATTACCTGAGGTCAGGAGTTCAAGACCAGACTG 4021 GTCAACATGGTGAAACTCCATCTCTACTAAAAATACAGAAATTAGCCAGGAGTGCTGGGT 4081 GGACGCCTGCAATCCCAGCTACTTGGGAGGTTGAGGCAGGAGAATTGCTTGAACCCAGGA 4141 GGCAGAGGTTGCAGTGAGCTGAGATCATACCATTGCACTCCAGCCTGCAAGACAGAGTGA 4201 AACTCTTGTCTCAAAAAAAAAAAAAGAAAAAAAGAAAGTGAGCCTGTTGATGTCTAGCTA 4261 ACCCATTGGTGGAGTCTGCATTGAATCTGTAAGAATATTGTTCTGGATCATGCAGGTGGG 4321 ATTTGTATAAAAAGCCTGTCCAACATTTAGTTCTCACTCGCATCAGAATCATACTGTTTT 4381 GAATTGCCATATAAGACATGCTCCATCATGACACTTGCTTCTCCTCCAAATACAAGTTGA 4441 GTGCTTAGTCCTTCACCTGCTAGAATCAGGATACAATCAACATGATTATGTTCCTTAACA 4501 CACTTGGATTTATGCAGATGTGGTGTGTCCTTTGCTCTTTTTTTTCTTAAGACAGAGTCT 4561 TGCTCTTGTCGCTCAGGCTGGAGTGCAGTGGTGTGATTTCGGCTCACTGCAACCTCCGCC 4621 TCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACCA 4681 CCACCATACCAGGCTGATTTTTTTTGTATTTTTAATATAGACAGGGCTTCATCATGTTGG 4741 CCAGGCTGGCAGGCTGGTCTCGAACTCCTCACCTCGTGATCCGCCCACCTCAGCTTCCCA 4801 AAGTGCTGTGATTACAGGCGTGAGCCACCGCGCCTGGCCTGCTCTTAAAACCCGTTATTC 4861 AGAAGATTTGTAAAAGAAATAGGCTTTTTTTTTTTTTTTGGTTAATTCAAACGAGGGGAA 4921 AATTAGATAGCATTTTCCCCTAAAGAAATGTTAATGTTCATTTTGTGGCTTTGTTTTCAA 4981 GTTTCAGGAGCCATGTACATCTCAGAAGTGTTACGAAGTGATTAGAAAAGGCCTCTGGTA 5041 AAAGACCTTCAAGGACCAACCATTATTATTGGTCACTTGTTTGAACTTGGCGCTTAAAAA 5101 AAAAGTTGGACTGTGATGCTTTGGTGCTCTTTATCACAGACATCAGTGATGGTCTGAAAC 5161 ACTCTCTAGCAGTTTATACGAAGAATGGACTTACATGTTATCAGTTACACTCATGATTTA 5221 CTCGTTCAGCTTCTACAAGTCCATACTTTATGCTGTCTCATTTAAGAGACAGTCCACTTC 5281 TGAAAATAAAATCGATAGTTGATGATACATTTTTGGCTAGCCAGCTTTCTGTAAAAGGCA 5341 AGTATGTTAGACATGCACAGTTTCCTTCAAATCAAATGTTTAATAAGGTACTTCAAGAAA 5401 CGCCTTTTTAAAAATCAAAGGTTTTGGCCAGGCATGGTGGCTCACGCCTGTAATCCCAGC 5461 ACTTTGGGAGGCCAAGGTGGGCGAATCACAAGGTCAGGAGTTTGAGACCAGCCTGGCCAA 5521 CATGGTGAAACCCCGTCTCTACTAAAAATACCAAAAATTAGCCGGGCGTGGTGGTGGGTA 5581 CCTGTAATCCCAGCTACTCAGGAGGCTGAGATGAGAATCACTTGATCCCAGGAGGTAGAG 5641 GTTGCAGTGAGCCGAGATTGCGCCATTGCACTTCAGCCTGGGCAACGAGTGAAACTCTCA 5701 AAAAAAAATTCATAGTAATAATAAAGTCCCTGCATGCAGTTTGGAAATAAAATTTAAACT 5761 CATTTATGTGTAATAAATATGTAATAATTCACAGTCACCATCAAATGTTGGAATCATTGA 5821 ATTGCATACTTGTACTCATTCCGGCCACATCATGAAGTGTTTAAAGATTTGTATTTGCTG 5881 TTGTCCCTATGTAGTTCTATTGATGTTTGGTTTTGTTTTTTGGGGTTTGTTTTGAGCCCA 5941 GGCTAGAGTGCAATGGCACAATCTCAACTCACTACAGCCTGGACCTCCTTGGCTCAAGCA 6001 GTCCTCCCACTTCAGCCTCCCAAGTAGCTGGTCTGCAAGTGCTCACCACCACACCTGGCT 6061 AATTTTTGTATTTTTTGTAGAGACAGAGCTCTCACTATGTTGCCTAGACTGGTCTCAAAC 6121 TCCTGGGCCCAAGCAATCCTCCCATCACAGCCTCCGAAGGTGCTGGGATTACAGGCATAA 6181 GCCACTGTGCCCAGCCTGTTTTTAATAATGATATTAAGTGGGTTTGGTTCATGTGTTATT 6241 AATCAGTGTTAATAATCGTACTTTTTTTTTTTTTTAAAAGAAACCATGGTATTCTAAAAT 6301 CAGGAGTCCAAATAAAAGAAAGTTCTCGGCTGTGCATGGTGGCTAACACCTGTAATCCCA 6361 GCACTTTGGGAGGCTGAGGTGGGTGGATCACTAGAGGTCAGGAGTTTGAAACAAGCCTGG 6421 CCAACATGGTAAAACCCCCTCTCTACTAAAAATACAAAAATTAGCCGGGCATGGTGGTGC 6481 ACACCTGTAATCCCAGCTACTCGGGATGTTGAGGCATGAGATTTCCTTGAACCTGGAAGG 6541 CAGAGGTTGCTGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGTGACAGAGTAAGA 6601 CTGTCGCCAAAAAAGAGGAAAAAAGTTATCCAGTGCAGTTTCTACAGAGATAAAAGAAGT 6661 AATAGTTCTGGCTGGGTGTGGTGGCTTATGCCTGTAATCCCAACACTTTGGGAGGCCAAG 6721 GCAGGTGGATCACATGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGCAAAACTGT 6781 CTCTACTAAAAATATAAAAATTAGGTATGGTGGCACGTGCCTGTACTTACAGCTACTTGG 6841 GAGGCTGAGGCATGAGATGACAATCTCTTGAACCCAGGAGGCGGAGGTTGCAGTGAGCTG 6901 AGATTGCACCACTGCACTCCAGCTTGGGTGATGGAGTGAGACTCAAATAAAAAAGGTACT 6961 AGTTCTGCATTTCAGAGTTGGCTTGTTGAACCAGGCTATATGCTTCCAAGATTTAAATGT 7021 TTTTCTGTATTATACTCTCAATTGTGTTTTAAAAAAATCTCTTACAGAAATCTCTACCTC 7081 AGGCACTAAGTGTTATGACATGGGTAGCATATTGATATTGAAAACTTAGCTAGGACTTCC 7141 AGCCTTTTAAGATAATTTAAATGTAAAATTAAATGGTTAACCAGCAATCTAATGTCATGT 7201 GGTGTGCAGTTTGGATATTGCATGAACAGCTAAGGAATCACCTGTTCTAGTGCCAAAGAT 7261 CACTCATTGCTAATTTTGTTCTGTACAGCTTATGTAATATTTTCATGGTGGAGACGGACT 7321 CTGTGTGCTCAGGGCCTTGTCTCTAGGAAGATTTTGTCAATTCCAAATACAGTTTTGAAG 7381 ATTCATTTGTGGTTTGTGTTTCCTTTCTCAGAATTCGAAACCAACTGAAAATCAGCTGCC 7441 AGGTAATGTCATATTGAGGATTTTACCCTATTTATATAAGATAATACCCAAGAGCTTAGT 7501 GATATACACAGAAAAGCTAATACGAAAATCTCTTCCTCATACATTTCACCTGTAAGTTGA 7561 TCTTGGTGGGAATACTTGAGCAGAGAAATTTCCAACTAAAGGTTTGGGCTTAAAAGATGC 7621 CTTTTTTCATAGCTGATTTGAAGCTCTTCTGGGACTACTGTGGCTAAATAGGTCAGCATA 7681 TTCGTGGCGTCGCAATCTGGGGAATATAGGTAGCCTGGCGGGGGAGGCTGGGGTCTAAGC 7741 TCAAAAGCTGGGAATGCACCCTTGACTTCCCTCGGTTCTGGAGGCCTTTCCTGGAGTGCC 7801 TTCTGCAAGGGACCCTTTGCGGCAGCAGAGTCAGCAGTGTCGTTGAAAAGCCTAATCCAC 7861 CTCCTGAGAAGGGAGCAGCAATGCCCTCTTGTTCCCTCAGACACGGGATAGCCGACTGGT 7921 AGGTCACGAACAGCTCACCCTCAGCAGCACAGATTGTAAACTGGTCAGCCAACCATATTT 7981 GGTAATGAATTTGTGAGCTATTTGAACGCTATTGGAGCAGTTAAATCACTCTGGATCTGT 8041 TAGTTTTGAAAGTAGATTCTTTGGCAGATTTGCCATCAGAACGGAGACTAATACCTGTGG 8101 CATCAAATCTGTTTTCTTTGCTACACAGAGTTTGAAAACTGTTTTTAAGGTTTTAAGGGC 8161 TTATATGATACTTTTAGGGTTTGTTTCCTTTAGATATCTCCCATTGGAAGCAGGTTGCAC 8221 AACGGCATGAAGAATATGGAAGCAGAGGCAAATTTGGGAACAGAATCCAGGTCTGTACAA 8281 TCCAGTATCCTTTGTTCTTGCAAGAGGAGGGTCCAGAAGCAGCTGTTAGCGGGTGCTCAC 8341 AAGGGTTTAGGGGCATCTTTGCTGGGTGGACCTTGCATCTGTCTATTATAATACTTTTTC 8401 AAAATGTAACCATTGGACCATTGGACTACTTGTTAGAAGTAACAGGAATGCTATTAACAT 8461 ACAGATTCCTAGGCCAAGGCGGGCGGATCACGAAGTCAGGAGATTGAGTCCATTCTGGCT 8521 AACATGGTGAAACCCCATCTCTACTAAAAATACAAAAAAATTAGCCGGCCATGGTGGCGG 8581 GCACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGG 8641 CGAAGCTTGCAGTGAGCCAAGATTGTGCCACTGCACTCCAGCCTGGGCAACAGAGCTAGG 8701 CTCTGTCTCAAAAAAAATAATAATTAAAAAATATATATAACAGAGAGAGATAGCGATATA 8761 TAGATTCCTAGTCTGGGCAACATAGTGAGACCCTGTCTCTACAAAAAATAAGCAAAAAAT 8821 TAGCTAGGCATGGTAGTACTCACCTGTAGTCCGAGCTCCTTGGGAGGCTGAGGCAGGAAG 8881 ATCGGTTGACCGCCAAGGTGGAGGCTACAGTGAGCCGTGGTTGCGCCACTGCACGCCACC 8941 CTGGATGACAGAGCAAGAGCGAGACCCTGTCTGTCTGTCTCTCTATATATATACGTGTGT 9001 GTGTGTGTATGTGTGTGTGTGTGTGTGTATTTTAGATCCATTGCAGACCTACATAATCAG 9061 AACTAACAAGTTCTCTGGGGGATTCATTTTTTTAAATCTTTTATTAAGATATAATTTACA 9121 TGGAGTAAAACTGTTGCATAGTTAGAAATGCATACAGTTAAGTAACCACACTAGAAAGAT 9181 GCATAATAGTTGCATTACCCCCAAATTACCTGGTGATTCTTATGTACATTATAGTTTGAA 9241 AACCACTCATTTTTAAAAAATTGTTTGAAGTAGCCAAAATAAAAGGGGAAAGAGAATTGT 9301 TTTGAATTACCTCCTCTGTTCAAGAAGGTTTAGACCTGGCATAGCACATGAGTTTCATCT 9361 CATCAGTCAGCTCAGAATGACTGCTGGCTCCCTGGAGGGGAGACCTACATAGAGGATCCA 9421 GGGACTCCTCTGGGTTCAGCCAAATAATCTATCAGTGATTTTGAGGTTGGGGCAGGGTTG 9481 ATGGAAGAGTGTGCCATCCTTGACTAGATGTTAAGCATGTTTCAAAATATCATTGATATG 9541 GGAGTGCTGGGAAGGGAAGAGGTGGTCCCTTTAAATGATATGGAAGGGGGGAAGGGAAGT 9601 GCTGCATAGAGGATGGCATGGTTCCTGGCTAGGGCTCCACCCCCACGGACCTAGGTGAGG 9661 ACTGGCATTTCCTGCCCAAATGCTTCGCCCCCATTCCCTAGACCCTAGTAGGCAGATGCA 9721 GGTGGCTGGATGTCCGGAGGAACACTGACAGGCACCGGTGCGCTGGCAGGCCACCGACCT 9781 GCAGAAGCAGAATGACATGGAGTTTGGCCTGGGCAGTCAGAGGAGAGCCCGGGCCGCCAA 9841 GCGGCCGGACTCCAGGGGAAAAGCATCTCCCTTCTGGCTCCCCCATCTGCTGAGAGCTAC 9901 TTCCACTCAATAAAACCTTGCACTCATTCTCCAAGCCCACGTGCGATCCGATTCTTCCAG 9961 TACATTAAGGCAAGAACCTCGGGATACAGAAAGCCCTCTGTCCTTGCAATGAGGCAGAGG 10021 TCTAATTGAACTAACACAAGCCGCCTATAGACGGCTAAACTAAAAGAGCAAAACTAAAAG 10081 CACACACTGTAACATACGCCCACTGGGGCTTCAGCTGTAAACATTCACCCCTGGACACTG 10141 TCTTGGGGTCAGAGCCCCACAGCCTGCCCATCTGTATGCTCACCTAGAAGTTTGAGGTTT 10201 GAGCAGCAGGGCACTGAAGAAGTGAGCCACTCTCCCATTGCACACCCTGCAAGGGGACAA 10261 GGGAACTTTTCCTGTTTCATCATGAAATAATGGATTTAGTGGCCGGGAGGGGTGGCTCGC 10321 GCCAGTAATCCCAGCACTTTAGGAGGCTGAGTCGGGTGGATCACGAGGTCAGGAGTTCCA 10381 AGACCAGCCTGGCCAAGATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCTGG 10441 GCGCAGTGGAAGGCGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAAGAGAATTGTTT 10501 GAATCTGGGGCAGGAGGGCAGAGGTTGCAGTGAGTCAAGATCGCACCACTGCACTCCAGC 10561 CTGGGTAACAGAGTGAGACTCCATCTCAAAATAAATAAATAAAATACGCATTTAGCACTT 10621 CGGGATCATGACTATGACTAGGCCCATGTGAAAAGAATCATCTCACCTTGTATGTAAGCA 10681 AGAGTTGGTGTCATTACTTAGATATTTCTCCTTCCTACAGTTAATGACATCTTGGAGGAA 10741 CTTAGAAACAGGAAGTGACTCAGGATTGGGAAGCACCAGAAAAAGAGCTGCTGACATGAC 10801 ATGGTGACAAGCATTCTTTGTCAATTGACTTTTTCTCCCGCCCCCTGGATCCTAAGGAAA 10861 CGGCACAGCATTTCCTTGCCCTGCCCTTGACGTGCTATGCCTACTCTAAGCTAGATAGGG 10921 GCTTCTGAAAGAAAAACCTAAGGAAGCCCCCTGCTCATGCCTGGAGGAAAGAACTGCCGA 10981 CTTTGTCTTTGCCCTCAGCCAAACCGCCACAAGTATCTTTGTCTGCCTGGCTCTCCTGGG 11041 GCCATCACAGAGGGCTGGGGATGCTGACAGCCGTAAACTCCAGGCGAGGCAGAACCAAGC 11101 AGCTGTTCATAAGCGATTTCAGTGCATGTCCAGTGACCCGAATGGGACCCGACCATGGGG 11161 AAAATGATACAGAACTAGATCGCTTTCTTTAAATTTTATTTTTAGTTTGTGTACTCCTAT 11221 GGAAGTGTAATTTTATCTTTGTGCTGGGAACACTGAAAAGCACTGACGGGGAGCTCCTTT 11281 GGCCTGGCCCATTTTTCTGAAAGCACGTTGCAGCTGTGGCTTCTGCCCAGCAGGCAGAGA 11341 CTGATGTTAGATCCTGTACCCAGCTGCTGCTGGGAGAAGGGACAGACTCAGCTTCTGACT 11401 GCAGGGGCATCTGCAGGAAGATCGGGGAAACTGAAGACACTTTTAACTGCAAAAGCCCTA 11461 GAAACAGTAAGTGCAAATTGTTCCGTGGATTGTTGACTTCACATTCAAACCATCGGGAAG 11521 TGATCGAGACTGGAAGCGTCGACAAGGATCCGCAGGGTCTGGGACCAGAGACCAGCTTGT 11581 GATCCTGCTGTGGAGTGCTAGGGGTCTGCTCTGGGGAGGGGGCTTTGGCCTAGAAGAGAC 11641 ATGGTACAGGGTGGGAACTGTTACAGTCATGCAACACTTAACATCAGGTGATTCTGTCAT 11701 TGTACAAATATAGAAAGAACTCACACTAACCCATACAGCGTAGCCTACTACACACGCAGG 11761 CTCTATAGCCTATTGCTCCTAGGCTACAAACACATCCAGTGTGTGTCTATACTGAATGCT 11821 GTAGACAATGGCAACACAATGGTATTTATATATCTAGACATATCTAAACAAAAGGTACAG 11881 TGAAAGATAAAATATATACAGTATAAAAGATTTTTTTAAAAAGCTGGGTGTGGTGGCACA 11941 CTCCTGTAATCCCAGCACTTTGGGAGTCCTGAGGTGGGAGGATCACTTCATCCCAGGAGT 12001 TCGAGACGAGCCTGGGCAACATGGCGAAAACCCATCTTGACAAAAAATACAAAAATTAGC 12061 CGGGTGTGGTGGTGGGCGCCTGTAATCCCAGCTATTCAGGAGACTGAGGCGTGAGAATCG 12121 CTTGAACCCGGGGGGCGGAGGTTGCAGTGAGCCAAGATCATGCCACTGCACTCTAGCCTG 12181 AGTGACAGAGTGAGACATTCTGTCTTTTTTTTTTTTTTTTTGAGACGGAGTTTCGCTCTT 12241 GTTGCCCAGGCTGGAGTGCAATGACGCGATCTCAGCTCACCTCAACCTCCGCCTCCCGGG 12301 TTCAAGCAATTCTCCTGCCTCAGCTTCCCGAGTAGCTGAGATTACAGGCACGCGCCACCA 12361 CACCCAGCTAATTTTGTATTTTTAGTAGAGACGGGATTTCTCCATGTTGGTCAGGCTGGT 12421 CTCGAACTCCCGACCACAGTTGATCCGCCTGCCTGGGCCTCTCAAAATCCTGGAATTACA 12481 GGCTTGAGCCACTGTACCCGGCAACATTCTGTCTTAAAAAAAAAAAACACGGGCCGGGCT 12541 CAGTGGCTCACGCCTGTCATCCCAGCACTTTGGGAGGCCGAGGCAGGCGAATCACGAGAC 12601 AGATCACCTGGGCAAGAGAGGGAGACTCCATCTCAAAAAAAGAAAAAAAGAAAATTAAGT 12661 CCCCCCCATGCTCCCGAGTCTTCTTACTCCTCATAGGTGACCACTGTTCAGTCTACCGTT 12721 TCTTCCCAGAAATATGTGCACATACAAGCATATACATATGTCTACTCTTTGCTTAGCAAA 12781 CCATTTATTCAGCCAAATTACAATTGTTGTCTTTGATAAAGCAGGCTACAGACCTCCAGA 12841 TCCTAAATTGGTGGCACCAAAATTAGAGAACTTACTTTGTCTTGAAGAGAACACTTGCAA 12901 ATTGACCAGGAACCCCTACTGCCAGTGTACATGAATTAGACTTCCATAAAGATTATAACT 12961 TAGGTGCCAGAATACACATTTTGAAATGAATGTAGGCCATCTAAAGGCATTTTTAGCTTC 13021 TAAACATTAACTTTATGGGGGCATAATTTACAGACAATAAATATACCTGCTTACAGTACA 13081 TAGAGTTCAATTAGTTTTTGTTTTGTTTTGTTTTGTTTTGTTTTGTTTTGTTTTGAGAAA 13141 GGGTCTTGCTCTGTTACTCAGGCAGAAGTGCAGTAGCATGATCATAGCTCACTGCAGCCT 13201 GGATCTCCTGGGCTCAAGTGATCCTCCCACCTCAGCCTCCCAAGCAGCTAGGACTACAGG 13261 CACATGCCACTACATCCGGCTAATTTTTAAATTTTTCTGTGGATATGGGGTTCTCACTGT 13321 GTTGCCCAGGCTGGTCTTGAATTCCTGGGTTCAAGTGATTCTCTTGTCTCAGCATCCCAA 13381 AGTGTTGAGATTACAAGTGTGAGTCACTGCACCCAGCTGACTTTTTTTTTTCCCACTGTA 13441 AATACCATTGCCTTCTTTTGAGGGGGTTATTCTGAGTTTTAATTACTTATTGTTTTCTGG 13501 TTATGAAGATACTACAAGTTCCTTATTTTAAAATAGAGACAATTCAGAAAATAAAGAGAA 13561 ACCAAAAGACATCAATAATTGCTCCATCCCTCAAAAAAAAAAAAATTTGGCATTATTAGC 13621 ATTGTCTTTTTAAAAGCATATATGTGGCCGGGCATGGCGGCTCATGCTTATAATCCTAAC 13681 ATTTTGGGAGGCCAAGGCAGGAGGATAGTTTAAGGCCGGGAGTTCAAGACCAGCCTGGGC 13741 AACACAGCAATTCTCCCATTTCCACACACACACACAGTTTAGGCCGGGCCGGGCGTGGTG 13801 GCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCAAGACGGGCGGGTCACGGGGTCAGGA 13861 GATTGAGACCATCCTGGCTAACACGGTGAGACCCCGTCTCTACTAAAAATACAAAAAAAA 13921 AAAAAAAATTAACCAGGTGTGGTGGTGGGTGCTTGTAGTCCCAGCTGCTCGGGAGGCTGA 13981 GGCAGGAGAATGGCGTGCACCCGGGAGGCAGAGCTTGCCGTGAGCCAAGATCGCGCCACT 14041 GCACTCCAGCCTGGGTGACAGAGCAAGACTTCACCTCCAAAAAAAAAAAAAAAATTTAGG 14101 CCGGGCATGGTGGCTCATGCCTATAATCTCAGCACTTTGGGAGGCTGAGGCGGGTGAATC 14161 ACCTAAGTTCAGGAATTCAAGACCAGCCTGGCCAACATGGCGAAACCCCATCTCTACTAA 14221 AAATACAAAAATTGGCTGGGCGTGGTGGCACACACCTGTGATCCCGGCTACTCGGGAGGC 14281 TGAGACAGCAGAATTGCTTGAACCCAGGAAACAGGTTGCAGTGAGCCAAGACTACGCCAC 14341 TGCACTCCAGCCTGGGCAATAAAGCAAGACCTTGTCACATACACACAAAAAATTGGCCAG 14401 GTGCGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGGGGCTGAGGCAGGCGGATCACGA 14461 GGTCAGGAGATCAAGACCATCCTGGCTAACATGGTGAAACCCCATCTCTACCAAAAATAC 14521 AAAAAAATTAGCTGGGCGTGGTGGCGGGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG 14581 GCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTGGCAGTGAGCCGAGATCGCACCACTG 14641 CACTCCAGCCTGGGCGACAGAGCAAGACTCCATCTCAAAAAAAAAAAAAAAAAAATTGGC 14701 TGGATGCGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGTGGATCA 14761 CCTGAGTTCGAGAGTTCACGACCAGCCTGACCAATGTGGAGAAATCCCCATCTCTACTGA 14821 AAATACAAAAAATTAGCCGAGTGTGGTGGCACATTCCTGTAATCCCAGCTACTCGGGAGG 14881 CTGAAGCAGGAGGACTGCTTGAGCCCAGGAGGTCAGGCTGCAGTGAGCTATGATCACGCC 14941 AACGCACTTCAGCCTCGGCAACAGAGTGGGATCTTGTGTCTAAAAAATAAAAACGAAAAA 15001 TAAATGCACACATGCACTTACTCATCTGTAAACACGGATAGTAATTTCAAGTGTCCTTAT

	Literature

Development:127/19/dev4359 PMID: 10976050

Defective vascular development in connexin 45-deficient mice. Olaf Krüger, Achim Plum, Jung-Sun Kim, Elke Winterhager, Stephan Maxeiner, Gaby Hallas, Susanne Kirchhoff, Otto Traub, Wouter H. Lamers and Klaus Willecke Development 2000 Oct;127(19):4179-93.