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List of -TEMs -PEMs -NEMs

TEMs TEM1 TEM2 TEM3 TEM4 TEM5 TEM6 TEM7 TEM8 TEM9 TEM10 TEM11 TEM12 TEM13 TEM14 TEM15 TEM16 TEM17 TEM18 TEM19 TEM20 TEM21_1 TEM21_2 TEM22 TEM23 TEM24 TEM25 TEM26 TEM27 TEM28 TEM29 TEM30 TEM31_1 TEM31_2 TEM32 TEM33 TEM34 TEM35 TEM36 TEM37 TEM38 TEM39 TEM40 TEM41 TEM42 TEM43 TEM44 TEM45 TEM46

Summary -Overview of TEMs -extracellular TEMs -intrac. & membrane TEMs -TEM tissue distribution

Supplementary -SAGE -Seq.analysis Methods

FAQ

Tumor Endothelial Marker Report: TEM3/TEM17

	TEM domain structure

	TEM Sequence Analysis

name	from	to	source/E	description
SignalP	1	18	SEG	LCR(LAR)
unknown	18	143	BlastP	This region has in part similarity to Drosophila and C. elegans proteins.
NIDO	143	290	SMART, E = 8.27e-01	Extracellular domain of unknown function in nidogen (entactin) and hypothetical proteins.
Plexin_repeat PS (Plexin_Semaphorin)	303	349	Pfam, E = 0.021	Low cystein spacing. Plexin like cysteine-rich repeats are found in several different extracellular receptors (plexins, semaphorins and integrins). The function of the repeat is unknown.
unknown	349	427		A LCR (S,T-; possibly mucin-like) is predicted for residues 365-400 by SEG25. Seven (out of 10) O-glycosylation site predictions cluster in the region 366-384.
TM	427	447	toppred2 = 2.4, SEG
cytoplasmic	447	502	Prosite, TYR_PHOSPHO_SITE	A potential tyrosine phosphorylation site can be found by Prosite at position 475: RSHPDHSTY. It has to be noted that second tyrosine phosphorylation site is predicted at position 83 and is assumed to be false-positive. No homologues can be found in this region.
502

	Remarks

• CLASSIFICATION: The protein is predicted to be a type I membrane protein, but this assignment has to be considered with caution as the protein sequence is not very reliable in all its parts.

• HOMOLOGUES: The sequence is not confirmed by full-length homologues in other species. The middle sequence part contains extracellular domains (PSI, NIDO) the organization of which is shared by the two major homologous proteins (Drosophila protein CG2221 (Identity= 43% in 87-352 of TEM17); C.elegans- C36E8.3 (Identity= 40% in 78-342 of TEM17)).

• DOMAIN ARCHITECTURE: TEM17 is a potential type I membrane protein. In its extracellular domain, Tem17 shares regions of homology with two other protein-families which have been implicated in axon migration:
  1. a more N-terminal region similar to parts of the G1 domain of nidogen
  2. a 100aa region with homology to Plexins (Tessier-Lavigne et al, 1999).
  A Psi-BLAST iteration(E=0.01) performed with the extracellular 420aa shows homologies clustering independently in the PSI and nido domains, with only two proteins (Drosophila CG2221 and C.elegans- C36E8.3) showing a similar domain-structure (CG2221 and C36E8.3 were excluded from all iterations.
     The short cytoplasmic domain shows no similarity to known proteins.

• FUNCTION: The NIDO domain is found in several non-collagenous human glycoproteins as alpha-tectorin, nidogen. A Psi-BLAST analysis was performed with this domain. No explicit function can be proposed for the NIDO domain.
     The ~ 100 aa region with homology to PLEXINs contains a short cysteine-rich motif, termed Met-related sequences (MRS), whose minimal consensus is: C-X(5-6)-C-X(2)-C-X(6-8)-C-X(2)-C-X(3-5)-C (Maestrini, 1996; Tamagnone, 1997). Semaphorins, the plexin family of semaphorin receptors, and scatter factor receptors share evolutionarily conserved protein modules, as the semaphorin domain and Met Related Sequences (MRS)(Winberg, 1998). All these proteins also have in common a role in mediating cell guidance cues. PlexinC1/VESPR, a divergent member of the Plexin family known to bind GPI linked semaphorin, shows closest similarity to the TEM17 plexin-like region. No explicit function can be proposed for the MRS domain.

	Sequence Information

TEM	TEM3
Reliability of Gen2Tag mapping	Cid->tag (Hs.13429) frequency: tem3: CATG-CATTTTTATC=9/9 (EST oriented)
Analysis	BlastP against nr Output Created by a Suite of Single Sequence Analysis Techniques
Protein Description	SEQ.SOURCE: gi|9857404|gb|AAG00869.1|AF279144_1 tumor endothelial marker 7
Protein Sequence	MRGELWLLVLVLREAARALSPQPGAGHDEGPGSGWAAKGTVRGWNRRARESPGHVSEPDRTQLSQDLGGG TLAMDTLPDNRTRVVEDNHSYYVSRLYGPSEPHSRELWVDVAEANRSQVKIHTILSNTHRQASRVVLSFD FPFYGHPLRQITIATGGFIFMGDVIHRMLTATQYVAPLMANFNPGYSDNSTVVYFDNGTVFVVQWDHVYL QGWEDKGSFTFQAALHHDGRIVFAYKEIPMSVPEISSSQHPVKTGLSDAFMILNPSPDVPESRRRSIFEY HRIELDPSKVTSMSAVEFTPLPTCLQHRSCDACMSSDLTFNCSWCHVLQRCSSGFDRYRQEWDGTMGCAQ EAEGQDVRGLPGMRTTTSASPDTSFSPYDGDLTTTSSSLFIDSLTTEDDTKLNPYAGGDGLQNNLSPKTK GTPVHLGTIVGIVLAVLLVAAIILAGIYINGHPTSNAALFFIERRPHHWPAMKFRSHPDHSTYAEVEPSG HEKEGFMEAEQC
SAGE-Tag	CATG-CATTTTTATCT
EST/cluster Description	UNIGEN CLUSTER: Hs.125036: tumor endothelial marker 7 precursor SEQ.SOURCE: gi|15987486|gb|AF378753.1|AF378753 Homo sapiens tumor endothelial marker 3 precursor (TEM7) mRNA MARKINGS: TEM3 Tag in red TEM17 Tag in mediumvioletred first Poly-A-signal following the Tag in bold
mRNA Sequence	GCGGCCGCAGCCTGAGCCAGGGCCCCCTCCCTCGTCAGGACCGGGGCAGCAAGCAGGCCGGGGGCAGGTC CGGGCACCCACCATGCGAGGCGAGCTCTGGCTCCTGGTGCTGGTGCTCAGGGAGGCTGCCCGGGCGCTGA GCCCCCAGCCCGGAGCAGGTCACGATGAGGGCCCAGGCTCTGGATGGGCTGCCAAAGGGACCGTGCGGGG CTGGAACCGGAGAGCCCGAGAGAGCCCTGGGCATGTGTCAGAGCCGGACAGGACCCAGCTGAGCCAGGAC CTGGGTGGGGGCACCCTGGCCATGGACACGCTGCCAGATAACAGGACCAGGGTGGTGGAGGACAACCACA GCTATTATGTGTCCCGTCTCTATGGCCCCAGCGAGCCCCACAGCCGGGAACTGTGGGTAGATGTGGCCGA GGCCAACCGGAGCCAAGTGAAGATCCACACAATACTCTCCAACACCCACCGGCAGGCTTCGAGAGTGGTC TTGTCCTTTGATTTCCCTTTCTACGGGCATCCTCTGCGGCAGATCACCATAGCAACTGGAGGCTTCATCT TCATGGGGGACGTGATCCATCGGATGCTCACAGCTACTCAGTATGTGGCGCCCCTGATGGCCAACTTCAA CCCTGGCTACTCCGACAACTCCACAGTTGTTTACTTTGACAATGGGACAGTCTTTGTGGTTCAGTGGGAC CACGTTTATCTCCAAGGCTGGGAAGACAAGGGCAGTTTCACCTTCCAGGCAGCTCTGCACCATGACGGCC GCATTGTCTTTGCCTATAAAGAGATCCCTATGTCTGTCCCGGAAATCAGCTCCTCCCAGCATCCTGTCAA AACCGGCCTATCGGATGCCTTCATGATTCTCAATCCATCCCCGGATGTGCCAGAATCTCGGCGAAGGAGC ATCTTTGAATACCACCGCATAGAGCTGGACCCCAGCAAGGTCACCAGCATGTCGGCCGTGGAGTTCACCC CATTGCCGACCTGCCTGCAGCATAGGAGCTGTGACGCCTGCATGTCCTCAGACCTGACCTTCAACTGCAG CTGGTGCCATGTCCTCCAGAGATGCTCCAGTGGCTTTGACCGCTATCGCCAGGAGTGGATGGACTATGGC TGTGCACAGGAGGCAGAGGGCAGGATGTGCGAGGACTTCCAGGATGAGGACCACGACTCAGCCTCCCCTG ACACTTCCTTCAGCCCCTATGATGGAGACCTCACCACTACCTCCTCCTCCCTCTTCATCGACAGCCTCAC CACAGAAGATGACACCAAGTTGAATCCCTATGCAGGAGGAGACGGCCTTCAGAACAACCTGTCCCCCAAG ACAAAGGGCACTCCTGTGCACCTGGGCACCATCGTGGGCATCGTGCTGGCAGTCCTCCTCGTGGCGGCCA TCATCCTGGCTGGAATTTACATCAATGGCCACCCCACATCCAATGCTGCGCTCTTCTTCATCGAGCGTAG ACCTCACCACTGGCCAGCCATGAAGTTTCGCAGCCACCCTGACCATTCCACCTATGCGGAGGTGGAGCCC TCGGGCCATGAGAAGGAGGGCTTCATGGAGGCTGAGCAGTGCTGAGAACACCAAGTCTCCCCTTTGAAGA CTTTGAGGCCACAGAAAAGACAGTTAAAGCAAAGAAGAGAAGTGACTTTTCCTGGCCTCTCCCAGCATGC CCTGGGCTGAGATGAGATGGTGGTTTATGGCTCCAGAGCTGCTGTTCGCTTCGTCAGCACACCCCGAATA TTGAAGAGGGGGCCAAAAAACAACCACATGGATTTTTTATAGGAACAACAACCTAATCTCATCCTGTTTT GATGCAAGGGTTCTCTTCTGTGTCTTGTAACCATGAAACAGCAGAAGAACTAACATAACTAACTCCATTT TTGTTTAAGGGGCCTTTACCTATTCCTGCACCTAGGCTAGGATAACTTTAGAGCACTGACATAAAACGCA AAAACAGGAATCATGCCGTTTGCAAAACTAACTCTGGGATTAAAGGGGAAGCATGTAAACAGCTAACTGT TTTTGTTAAAGATTTATAGGAATGAGGAGGTTTGGCTATTGTCACATGACAGACTGTTAGCCAAGGACAA AGAAGTTCTGCAAACCTCCCCTGGACCCTTGCTGGTGTCCAGATGTCTGCGGTTGTCAGCCCCTTCCTTT CCCCCGACCTAAACATAAAAGACAAGGCAAAGCCCGCATAATTTTAAGACGGTTCTTTAGGACATTAGTC CACCATCTTCTTGGTTTGCTGGCTCTCCGAAATAAAGTCCCTTTCCTTGCTCCAACTCCTTGTCTCTCAA CGTATTGGCTATGACGCAGCAAGCAGAATGAATTTGGACTCAGTTACAGGCTGTCAATGGTCTGCTCTGT AGCAGTCTCAGAGCCTCCCCGACCCACTACCTGGAGATAGCCAGATAGCCAGATGCCCTGCTCCTGGCCA CCTTTAAAGCCCCTGCATATGACACAGGTTAACTAAAGTCAAGATTGGGGCTGCTGCATTCCAGGTTCCC TAGACTCACAAGCTGGTCCTTGGCCAGGTGCAGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTG AGGCAGGCGGATCACCTGAAGTCAGAAGTTTGAGACCAGCCTGGCCAACATAATTAAAATGTCTCTACTA AAAATACAAAAAATTAGCTGGGTGTGGTGACGCTTGCCTGTATCCCAGCTACTCAGGAAGCTGAGACACG AGAATCACTTGAACCTGGGAGGCAGAGGTTGCAGTGAGCTCAGATAGTGCCACTGCACTCCAGCCTGGGT GACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAGAAAGCAGAACCTCATGGCTATAGAGTTGGCATTTT AGCCCCAGCTTCTGTAGCTCTGAAAGCCTAAAGAAGGTATTCTCTCCATCTGTTAAACACAGTATAGTGG CTCTCAGCCCTTGGGGCATGTTATCATGGGAGGGAAGTCAAATAAGAGGAGAGAAAAGAACTCAAGGGGG AAACTGCATTTTTAGGCTTTGCTCTCTTACCTTGCCCTTTCTACTCAGAACCAATAACTTCTGCATCAAA ACATGTTACAGCCTGCATCAAGGGCTTTACCCCAACCTGCAGCCCAGCCTTCCCTGGGTGAGCTTGCTAT GCGCAGCCACATTTACCATGTGGGGCTCCCTATTCTGATGGCCTGTTCGGTGCCGGGTTTACTCACTGCC CTGTTCTGATGTCAGTGCCTGTACATACCTCCAAAGGCAGGACTTGCCTGATAAATATTTTTCCTCCTCT GAACTGGATTTTATAGGCATTAAAGACAAGTCGGGTGGCTAGAGGGCTCCTTGAGACATACCTAGCAGGG AACTGCAGGTGGATTCTGTTGAGAGGCAAAGCACCTGAGTGGTTGGGACACAGGCAGCTGGCATGGGAGG GACTTTTTTTGAGACAGGGTCTCACTGTGTCGCCCAGGGCAAGGATGCCCAAAGACACCAGGTTGGAGAG GCACCTGCCAACTACTTGCTTTCCCTGGAGCCTGCATGTGCCTGTGGGGTGGGGAGGCGTAGGGGTCTAC GGCTGCCTGAGATGGGTGTGCACAGTGTGTGAAGTACCTACCTCCTTGCCTTGCTGGACTGTCAGCCAGT CGCAGGGCCGGCCACAAGACCCATGTCTCCATCTGGTCATACTCCATAGCTACCAAGTTAACCTGCTCTA AACTTTGGAGAACTGGATCTGTCCAATAAACGCTTATTTGGCCAAGCCTGATGGCTCGTGCCTGTACTCC CAGCACTTTGGGAGGCTGAGGTGGGAGGGTTGCTTGAGCCCAGGGGTTTGAGACCAGCTTGGGCAACAAC AACAAAAATGCCAGGTGTGGTGGGGTGCACCTGTAGTCCCAGCTACTAGGGAGGCTGAGCCAGGAGGATC ACTTGAGCCCGGGAGGTTGAGGCTGCAGTGGGGGGTCATAATCATGCCACTGTACTCCAGCCTGGGTGAC AGAGTGAGACCCTGTCTCCGAAAAAAAAAAAAAAAAAAGAACGGAAAAAGAAATGCTTACATTGTCAGGG ATCCTGTAGACAATCATTAACTCTATGAGATGCTTGGTTCTATTTTTTTGGGAGACTTTGTCCAAGTGTT TTGGCTTAAGAAATCCATAGGCCTCTCTTGGTGACACATCTCTAGTACTTTTTGTCATAAACAAACAGGC CATCTGCCGCCAAATACATCCACTCCCCATGCCACTGACATCCTATGGGTCAGCCAGGCTTGCTTTGACT GAGGCCGAGGCATCTGGAACTTTCTCTGCCTGCAGGGGCTAGCAGCAGAGGCTTCACCGCATCACCACCC CTTCCTCCACTCCTGACATTCTTTCCCTTCAGGGATCCAAAATGGTTGGCCGAGCTCCCAGTGGGAAAAC GTGTGCTAGAGTTGGGGAGTGAGATGAGTGGTGCTGTCCATGGAATCAGGCCACAGCAGGAACTGCCCCA CTGGCCATTTGAGACACACACAGGTGGTAAATGCTCTGCTGGTGGGCTGTGCTTCCCTCATTCAGAGAGC TCTGTTACAGCCCACTGTGTCCTTTAGAAGCTTGAAAGGAACCCAACTCTTTGCTGCACTGTCCTTTTTC TTCCTCAAATTCAGACCCTCCTTCCACCGGCACCCCCCTACTCCACCCTCAGCTCTTCCTTGCCTGGTTT ATCAAGCAGAGCTGAGGCCCCACGTTTCCAACTCTGATTGTCACTTGCATCTTCACAAAGGATAAACCAC GGAGCAACTGGAAAACCATCAGCCAAGCGTTCGGATGAGTCTGGTTATTGGTCCACCCCCGACCAGATTC CCTTACACTTAACTCACTTCTTTCTTTGGCAATGACCCTCATGACATGTATAAATGGGTATGACTAAGAA GAGGCTGTGATCTAACATTTATTTGCTGCCATTTTTTACTCTGGGGAGAAGCAGCCCCAACTCATCACTG GGAAAGAACTCCCCCTGCAAACCAGCTAAATTTGATAATTTAAACCCCCTGCCCCTAAAACTTCTCACAG AGCTGGGGAGTTGGTGGCAACTTTCCAAGTCAAGGTCTTGCTTAGAAAGTCCTTCACTACATGGCCAGGT GCAGTGGCTCACGCCTGTAGTCCCAGGTACTTGGGAGCCTGAGGCAGGAGGATTGCTTGAGCTCAGGAGT TCAAGGCTGCAGAGAGCTATGATCATCCCACTGCATTTGTTTAAAAATAAATTTTTAAAATTTGTGTGTT TTATCAGGGGTCTCCTGTACAGTGTATCTGTGTATGTTTGTGTGTGTGTTTGTATACAGCCTTGTTTAAT GTTTTGAGCAATAAGATATGCACACACAGGTATTTTGTTGCTAAAGAGATTGGACAAGGTTGTAGCTGTG CTCAGGCTTCAGCTTGGTTTGTTAAATTGAGAGATAAACAATGACAAGAGCTGCCAGCCAACCACACTAT TCAAAAAGCAAAGTGTTCACCACTAAAGCTAACCATTCATCTGGTTGCAGGCAAGGCTAAGGCTCTCTCT CCTCTAGTTCCTGGAACAGACTCACAGATTGGCATGAAGCACTGATCAGGGGCTGCACTCAGACTCCCTG GCCAAGCAAACCTACACCAGAAGAGTCAGTGTCACAGATATGATGCGGCCAATCTCTGTCTCCAAAAACC TACCTGAACTTAATGGTAGAATTCAAAGATCTGGGGACTGAGGGCACCCAGCCTTCTAAAACACAATGTA TTCATGTGTTTAGTGTAAACTCTCTGCATGGATTCTCAGTGTTAATAATAAAAGGAAGCATTCTTTTACA ACTCCTGCTGTGTGCAAAAGAAAGTGCAAAGGATTTGGAGTGGCATTCCGAAGATCACCACACATACCTT GGTTCTGATGGCTGCTGAACTCCGACTTCTTCGCTGAGACATGACTGTGGGAACAGCCTCCAGCTATCTG CTCATCAGAGGTGCTTTCCTCAACCTCCTGCACCACCTCCAAGAGAAACAGCCTAAAAAGAAACCCCAGC TGTTTACTTATATTGGTCTGTAAATCCCTGGAAGTAAACCCCATGCATTTTTATCTACTGTCTGAGGACA TACAATAAATCTGAGAAAGTCTATGCTGTCAAAAAAAAAAAAAAAAAAAA

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Angiogenesis

List of -TEMs -PEMs -NEMs

TEMs TEM1 TEM2 TEM3 TEM4 TEM5 TEM6 TEM7 TEM8 TEM9 TEM10 TEM11 TEM12 TEM13 TEM14 TEM15 TEM16 TEM17 TEM18 TEM19 TEM20 TEM21_1 TEM21_2 TEM22 TEM23 TEM24 TEM25 TEM26 TEM27 TEM28 TEM29 TEM30 TEM31_1 TEM31_2 TEM32 TEM33 TEM34 TEM35 TEM36 TEM37 TEM38 TEM39 TEM40 TEM41 TEM42 TEM43 TEM44 TEM45 TEM46

Summary -Overview of TEMs -extracellular TEMs -intrac. & membrane TEMs -TEM tissue distribution

Supplementary -SAGE -Seq.analysis Methods

FAQ