Tumor Endothelial Marker Report: TEM16 (tensin-like)

TEM domain structure

	TEM domain structure

TEM Sequence Analysis

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name from to source/E description

ABD-II 1 148 The homologous region is described as an actin binding domain in chicken tensin (proposed by similarity from chicken tensin - Lo, 1994).

364 896 SEG The central region of the protein is heavily interspersed by LCRs.

SH2 932 1047 Pfam, E = 9e-13 SH2 domains function as regulatory modules of intracellular signalling cascades by interacting with high affinity to phosphotyrosine-containing target peptides in a sequence-specific and strictly phosphorylation-dependent manner.

PTB 1072 1198 Aravind SDB, E = 4e-18 It has been previously proposed that subunit self-association occurs via this C-terminal region as it is the case for other actin cross-linking proteins (with the N-terminus being involved in actin-binding).

1205

	TEM Sequence Analysis

name	from	to	source/E	description
ABD-II	1	148		The homologous region is described as an actin binding domain in chicken tensin (proposed by similarity from chicken tensin - Lo, 1994).
	364	896	SEG	The central region of the protein is heavily interspersed by LCRs.
SH2	932	1047	Pfam, E = 9e-13	SH2 domains function as regulatory modules of intracellular signalling cascades by interacting with high affinity to phosphotyrosine-containing target peptides in a sequence-specific and strictly phosphorylation-dependent manner.
PTB	1072	1198	Aravind SDB, E = 4e-18	It has been previously proposed that subunit self-association occurs via this C-terminal region as it is the case for other actin cross-linking proteins (with the N-terminus being involved in actin-binding).
1205

Remarks

	Remarks

HOMOLOGUES: The C-terminus of the protein shows over 70% homology to the C-terminus of chicken, human (TEM24), bovine tensin. The overall sequence similarity between tensins in general is around 60% with higher identity close to the N- and C-terminus the identity (in this case it is around 45%). It can be assumed that the proposed TEM16 is just a fragment of a paralogue of human tensin.
DOMAIN ARCHITECTURE: In tensins the N-terminal (1-460) and C-terminal (1403-1735) regions contain known domains: PTPII in the N-terminus, SH2 and PTB in the C-terminus. These regions are devoid of LCRs (SEG25), while the central region is heavily interspersed by LCRs. Three regions with actin binding activity are found in the N-terminal and central region.
The proposed TEM16 fragment lacks the N-terminal 247aa if aligned to human tensin. The full-length protein is assumed to be functionally similar.
FUNCTION: If it is assumed that TEM16 is only a fragment of a complete tensin, functions typical for tensins can be hypothesized for the protein. Thus, TEM16 could play a role in integrating signal transductions with the cytoskeleton.
EXPRESSION CONTROL: TEM16 was recently cloned as a thyroid specific PTB domain protein.

Sequence Information

TEM TEM16

Reliability of Gen2Tag mapping

Cid->tag (Hs.12210) frequency:
tem16: CATG-TTAAAAGTCA=74/80(EST: oriented)

Analysis
BlastP against nr

Output Created by a Suite of Single Sequence Analysis Techniques

Protein Description

SEQ.SOURCE: gi|15987493|gb|AAL11993.1|AF378756_1 tumor endothelial marker 6 [Homo sapiens]

Protein Sequence

MVKCYHKKYRSATRDVIFRLQFHTGAVQGYGLVFGKEDLDNASKDDRFPDYGKVELVFSATPEKIQGSEHLYNDHGVIVD YNTTDPLIRWDSYENLSADGEVLHTQGPVDGSLYAKVRKKSSSDPGIPGGPQAIPATNSPDHSDHTLSVSSDSGHSTASA RTDKTEERLAPGTRRGLSAQEKAELDQLLSGFGLEDPGSSLKEMTDARSKYSGTRHVVPAQVHVNGDAALKDRETDILDD EMPHHDLHSVDSLGTLSSSEGPQSAHLGPFTCHKSSQNSLLSDGFGSNVGEDPQGTLVPDLGLGMDGPYERERTFGSREP KQPQPLLRKPSVSAQMQAYGQSSYSTQTWVRQQQMVVAHQYSFAPDGEARLVSRCPADNPGLVQAQPRVPLTPTRGTSSR VAVQRGVGSGPHPPDTQQPSPSKAFKPRFPGDQVVNGAGPELSTGPSPGSPTLDIDQSIEQLNRLILELDPTFEPIPTHM NALGSQANGSVSPDSVGGGLRASSRLPDTGEGPSRATGRQGSSAEQPLGGRLRKLSLGQYDNDAGGQLPFSKCAWGKAGV DYAPNLPPFPSPADVKETMTPGYPQDLDIIDGRILSSKESMCSTPAFPVSPETPYVKTALRHPPFSPPEPPLSSPASQHK GGREPRSCPETLTHAVGMSESPIGPKSTMLRADASSTPSFQQAFASSCTISSNGPGQRRESSSSAERQWVESSPKPMVSL LGSGRPTGSPLSAEFSGTRKDSPVLSCFPPSELQAPFHSHELSLAEPPDSLAPPSSQAFLGFGTAPVGSGLPPEEDLGAL LANSHGASPTPSIPLTATGAADNGFLSHNFLTVAPGHSSHHSPGLQGQGVTLPGQPPLPEKKRASEGDRSLGSVSPSSSG FSSPHSGSTISIPFPNVLPDFSKASEAASPLPDSPGDKLVIVKFVQDTSKFWYKADISREQAIAMLKDKEPGSFIVRDSH SFRGAYGLAMKVATPPPSVLQLNKKAGDLANELVRHFLIECTPKGVRLKGCSNEPYFGSLTALVCQHSITPLALPCKLLI PERDPLEEIAESSPQTAANSAAELLKQGAACNVWYLNSVEMESLTGHQAIQKALSITLVQEPPPVSTVVHFKVSAQGITL TDNQRKLFFRRHYPVNSVIFCALDPQDRKWIKDGPSSKVFGFVARKQGSATDNVCHLFAEHDPEQPASAIVNFVSKVMIG SPKKV

SAGE-Tag CATG-TTAAAAGTCAC

EST/cluster Description

UNIGEN CLUSTER: Hs.12210 : Homo sapiens cDNA FLJ11289 fis, clone PLACE1009621

SEQ.SOURCE: Homo sapiens tumor endothelial marker 6 (TEM6) mRNA

MARKINGS: TEM16 Tag in red
first Poly-A-signal following the Tag in bold

EST/cluster Sequence

1 TGCTCGAATGGACCATATCTCGGGAGCCTGACGGCCTTGGTGTGCCAGCATTCCATCACG 61 CCCTTGGCCTTTCCGTGCAAGCTGCTTATCCCAGAGAGAGATCCATTGGAGGAAATAGCA 121 GAAAGTTCTCCCCAGACGGCAGCCAATTCAGCAGCTGAGCTGTTGAAGCAGGGGGCAGCC 181 TGCAACGTGTGGTACTTGAACTCTGTGGAGATGGAGTCCCTCACCGGCCACCAGGCGATC 241 CAGAAGGCCCTGAGCATCACCCTGGTCCAGGAGCCTCCACCATGTGTCCACAGTTGTGCA 301 CTTCAAGGTGTCAGCCCAGGGCATCATCGCTGACAGACAATCAGAGGAAGCTCTTCTTCC 361 GGAGGCATTACCCCGTGAACAGTGTGATTTTCTGTGCCTTGGACCCACAAGACAGGAAGT 421 GGATCAAAGATGGCCCTTCCTCAAAAGTCTTTGGATATGTGGCCCTGGAAGCAGGGCAGT 481 GCCACGGATAATGTGTGCCACCTGTTTGCAGAGCATGACCCTGGAGCAGCCTGCCAGTGC 541 CATTGTCAACTTCGTATCAAAGGTCATGATTGGTTCCCCAAAGAAGGTCTGAGAACTCCC 601 CTCCCTCCCTGGACCCACCGATGCCTCTCGAAGCCCTGGAGACAGCCGTTGGGTGAGGGT 661 GGGGCCCCCACTTTTTACCAAACTAGTAAACCTGACATTCCAGGCCCATGAGGGGAAAGA 721 GGATCTTCCAGCTCTGCAAAAACAAGAACAAACAACATCACCGTGAATTGGCCTTTCCTG 781 AAAGTGACTTATCTGACACATCTCTGTAGCCACATGCTTTTTGGGTAGAAGAAGCTGGGC 841 ATGGGTGCACCCCACCCCCTAGGGTCCCCATGGGAAAGGGACAAGCAAGGAAACAGCACA 901 GAACACGAGGTGGTCCCCATGTCCCTGGCACACTAGCATTCCGGGGGATGAGGAATCCCC 961 AGCCCTTGAGGCAGAGGTGCCGAGTGACTGCCATGCTTCGCCCGTCCGCATGGGCGCTTC 1021 TGTCCAGCTGCACCCGAGGCCGGGGGTTTCCCTCACCTCGGTCTTCCCAAGATGGAGATG 1081 CTAACGAAACTGAGAAGGGGGCGTATGTTTGACGAAGGTTTGTGCAAGTCAGGCCCTTCT 1141 GGAACACAGCAGGGCCTACAACGAGGGGCCTTTGCGATGGGCTGTGAGGATGGGGGTGGT 1201 GGGAAGAATTGGCCACGTTAGAGACCCCATGCCACCCCACCATGGTGAGTGCTCTGTGCC 1261 TCCTGCTCACCTGTGGTGAGCTGGGCGAGCTGGGCGAGCTGGGCGAGCTGGGCTGGGGAG 1321 AGCCTGTGAGGACCGAGAGGAGAAATGAGAAGAAGGAACAAAAATATTATTTCTATGTAA 1381 TTTATATTTTACTTATGCCAAATTATTTATGATAATTTGCCATTGCTATACTGTACCAGT 1441 GTCAAATGCTGCAGCCTGCCAAGCTGTGATTTTGTGAGGCTTGTCCCTATGTAGGATGCA 1501 CCGCAGGCCCCTGGCCACTGAAAGAGTGTGCAGTGGACTGTGGGTCTCCCATATGCGGTG 1561 CCGCCCAAAGGTGGCTTTGCCTCAAGCAACCTACCCTGATGTTTTACTCATTGGAATGTT 1621 TTTCCCCGATTGTGGATGACTTCTTTTCTGATGGAGAGAGTCCAGGAGGGATGGAAAACT 1681 CCTGGATTTAAGCTCAGCATCCCCCACATGGGCTTTTCGATCATCTTCAGGCCTGAAGCT 1741 GCACGACCTGAAGTTCGCCTGCATTTATCAGCCCTCTTTGTGCTGCTCCTTGCCACCTTG 1801 GGGTTCCTGCTGGGGACCATGTGTGGTTGTGGCATGTGTGAGCAGAAGGGAGGATGAGGA 1861 AAAAGAGAAGAAACCCCGGTACTGACAAGCTGTTTTTGAGTGCCACTGTTTGCCATCATC 1921 TAAGCCACTGAATCAAGTGTATTTCAGGCTTATTTCAACATTCCAATGCCCTGGTTTTCC 1981 TGCTTGAATCTGTTCGTGGTCAAAGGTTTGGGGGAATTTGTGACCCTGGAACATCCCCAG 2041 AGTGAAAGATGGAGCTGGGCCACATCAGAATAAGGCCTTGGCCCCATCCTCTCACAGCCT 2101 AGGTGCTCTGCAGGCATGGCTGACTGTCCTGATTGCGATCCAGCCCGAAATTCCCTCCTC 2161 TGCTTTCAAAAGTCAAATCCCCCATTCTTAGGCCACACTGGTGTCACAAGCTCCTGTCAG 2221 GGAGCTGGGGTTTGGGAATGTGCTTTGTGAACTCTGCTTTAAAGTGAGGGGCCGAGGAAA 2281 ACTTAGAAACAGGCAGAGTTGGAAGCAGCCAAATCACAGTGGGTGTTGTGTGTGTGTGCG 2341 TGTGTGCATGCGTGCGTGTATGCGTGTGTGAAAGCAGGTGGACCATTCCACTTTTTAGCT 2401 CCTATTGATGCACCAAACCAAGTGCCTCATTTCTGTGCCAAATGTTTGCCTTGGTCGTTG 2461 TGGACCTCCTTCTCTAACTTGCGGTGGCATGACTGTCAGGAGGTGCTGGCATTTTCAGCA 2521 GATCCTCATGTGTTGACCCTGATGTCTTTAGCAGAGGCCTCTAGCATCTCGGTTTTTCAT 2581 CCACTGCAGGAATGTGGCCACAGGGAGCAGAGGTTTGTACTTTCCCCAAGAGGTCCTCAT 2641 CCTGAGACGGTCTCTACCCATGTTTAACCCAAAGAGTGCAGGCCAGGTTCCTTATCCTTC 2701 TGATGAAGGATGAGAGAGCTCATTTAGAAGTCAGAGCAAACTAGGGTCTCAGTATTGAGA 2761 AACGCAGCCTGCCAGGGAATCACAGAGACATCGGGGTGCCCGCGATGGCCCTCATGAAGC 2821 CATGCCTCGACGGCATTCAGGAAGCCCTGCAAACGTGCTTTTTGAACTCATTGGCCAGGT 2881 GTGATTTTTACACAAGGTAAACGTGGTCAAGGGCATCGGGGAATTTGCTCCAAGCAGATA 2941 GCTCCCTCTGAGGGAACCAAAGGAAGCAAGTTTCCACGATTTCTGAAGAGCTGGTATAGG 3001 AAGTTTCTTTCTTCCTTTTGTGTTAACATGTGCATTAAACAGAACAAGCTGTGTGTCATC 3061 ACAGATTGTACTGTGGGCTCAGAAACCGTGAGAGAGCCCCCACCGTGGACACCGGCTCTA 3121 GGGCCACAGGAAAAGGAACGTTTCCAGGCATTTTGTCTCCAGGGCTCCCGCTGGACAGGC 3181 ACGTACTGCCCCTGGGGAGTAAATGCGGAGAGTTCACGAACTGTGCCCAACGCATGTTAT 3241 AGCCAGGGTCCTACTAACTACTCAGTAAAAGAACGTATTGTTGTATTCCTCCAGTGTTAA 3301 GCTATAGCCCATGTTAAAAGTCACTGTGCATTTATTCTCAGCATCAAATACCTTGTAACG 3361 TCTTCTCTGCCTTGTTAGTGCATATTTTTACTTTTCTGATACTGTAAAGAATATATCCAG 3421 TATGTAAATGAATGTTCTATAAATCTTTTGTATAGTCATTTTCTCTGCTCCTTAAATATC 3481 ATCTCTATTCAGAGTATAATAAAATTATGAACTTGGTAAGCCTC

Genomic Sequence Description

Genomic Sequence

	Sequence Information

Literature

	Literature

PubMed: 95189829 Structure and function of SH2 domains.
Marengere LE, Pawson T.
J Cell Sci Suppl 1994;18:97-104.

PubMed: 10860821 Tensin can induce JNK and p38 activation.
Katz BZ, Zohar M, Teramoto H, Matsumoto K, Gutkind JS, Lin DC, Lin S, Yamada KM.
Biochem Biophys Res Commun. 2000 Jun 16;272(3):717-20.

PubMed: 10704455 Integrin dynamics and matrix assembly: tensin-dependent translocation of alpha(5)beta(1) integrins promotes early fibronectin fibrillogenesis.
Pankov R, Cukierman E, Katz BZ, Matsumoto K, Lin DC, Lin S, Hahn C, Yamada KM.
J Cell Biol. 2000 Mar 6;148(5):1075-90.

FAQ

PubMed: 95189829	Structure and function of SH2 domains. Marengere LE, Pawson T. J Cell Sci Suppl 1994;18:97-104.
PubMed: 10860821	Tensin can induce JNK and p38 activation. Katz BZ, Zohar M, Teramoto H, Matsumoto K, Gutkind JS, Lin DC, Lin S, Yamada KM. Biochem Biophys Res Commun. 2000 Jun 16;272(3):717-20.
PubMed: 10704455	Integrin dynamics and matrix assembly: tensin-dependent translocation of alpha(5)beta(1) integrins promotes early fibronectin fibrillogenesis. Pankov R, Cukierman E, Katz BZ, Matsumoto K, Lin DC, Lin S, Hahn C, Yamada KM. J Cell Biol. 2000 Mar 6;148(5):1075-90.