analysis of sequence from tem2
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
>gi|9857402|gb|AAG00868.1|AF279143_1 tumor endothelial marker 2 [Homo sapiens]
MPASLALLQPRAMMKTLSSGNCTLSVPAKNSYRMVVLGASRVGKSSIVSRFLNGRFEDQYTPTIEDFHRK
VYNIRGDMYQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTK
EAAELPMVICGNKNDHGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTNVDEMFYVLFSMAKLPHEMSP
ALHRKISVQYGDAFHPRPFCMRRVKEMDAYGMVSPFARRPSVNSDLKYIKAKVLREGQARERDKCTIQ
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
sec.str. with predator
> gi|9857402|gb|AAG00868.1|AF279143_1
. . . . .
1 MPASLALLQPRAMMKTLSSGNCTLSVPAKNSYRMVVLGASRVGKSSIVSR 50
___EEEE_HHHHHH_________EEE_____EEEEEE_________EEEE
. . . . .
51 FLNGRFEDQYTPTIEDFHRKVYNIRGDMYQLDILDTSGNHPFPAMRRLSI 100
E_____________HHHHHHHHH______EEEEEE_______HHHHHHHH
. . . . .
101 LTGDVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKEAAELPMVIC 150
____EEEEEEE_____HHHHHHHHHHHHHHHHH_HHHHHHHHHH_EEEEE
. . . . .
151 GNKNDHGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTNVDEMFYVLFS 200
________________HHHHHH______EEEEEEE_______EEEEHHHH
. . . . .
201 MAKLPHEMSPALHRKISVQYGDAFHPRPFCMRRVKEMDAYGMVSPFARRP 250
HH________HHHHHHHHH_________HHHHHHHHHH__EEE_______
. .
251 SVNSDLKYIKAKVLREGQARERDKCTIQ 278
___HHHHHHHHHHHHHHHHHHHH_____
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
method : 1
alpha-contents : 40.5 %
beta-contents : 21.9 %
coil-contents : 37.6 %
class : mixed
method : 2
alpha-contents : 35.8 %
beta-contents : 15.8 %
coil-contents : 48.4 %
class : mixed
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
GPI: learning from metazoa
-13.30 -3.19 -3.01 -2.07 0.00 0.00 0.00 -0.72 -2.37 -8.39 -2.95 -12.00 -12.00 -8.00 -12.00 0.00 -79.99
2.31 -2.44 -3.17 -1.12 0.00 0.00 -28.00 0.00 -1.23 -9.86 -2.95 -12.00 0.00 0.00 -12.00 0.00 -70.47
ID: gi|9857402|gb|AAG00868.1|AF279143_1 AC: xxx Len: 210 1:I 200 Sc: -70.47 Pv: 5.760329e-01 NO_GPI_SITE
GPI: learning from protozoa
-25.51 -2.78 -2.04 -1.61 -4.00 0.00 0.00 0.00 -0.73 -5.18 -10.39 -12.00 -12.00 -8.00 -12.00 0.00 -96.25
-26.47 -0.98 -0.01 -0.08 -4.00 0.00 0.00 0.00 0.00 -5.00 -10.39 -12.00 -12.00 -8.00 -12.00 0.00 -90.93
ID: gi|9857402|gb|AAG00868.1|AF279143_1 AC: xxx Len: 210 1:I 184 Sc: -90.93 Pv: 7.130055e-01 NO_GPI_SITE
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
# SignalP euk predictions
# name Cmax pos ? Ymax pos ? Smax pos ? Smean ?
gi|9857402| 0.304 118 N 0.259 118 N 0.659 6 N 0.176 N
# SignalP gram- predictions
# name Cmax pos ? Ymax pos ? Smax pos ? Smean ?
gi|9857402| 0.457 15 N 0.243 15 N 0.890 9 Y 0.437 N
# SignalP gram+ predictions
# name Cmax pos ? Ymax pos ? Smax pos ? Smean ?
gi|9857402| 0.377 143 N 0.217 116 N 0.917 42 N 0.382 N
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
low complexity regions: SEG 12 2.2 2.5
>gi|9857402|gb|AAG00868.1|AF279143_1 tumor endothelial marker 2 [Homo sapiens]
1-278 MPASLALLQPRAMMKTLSSGNCTLSVPAKN
SYRMVVLGASRVGKSSIVSRFLNGRFEDQY
TPTIEDFHRKVYNIRGDMYQLDILDTSGNH
PFPAMRRLSILTGDVFILVFSLDNRESFDE
VKRLQKQILEVKSCLKNKTKEAAELPMVIC
GNKNDHGELCRQVPTTEAELLVSGDENCAY
FEVSAKKNTNVDEMFYVLFSMAKLPHEMSP
ALHRKISVQYGDAFHPRPFCMRRVKEMDAY
GMVSPFARRPSVNSDLKYIKAKVLREGQAR
ERDKCTIQ
low complexity regions: SEG 25 3.0 3.3
>gi|9857402|gb|AAG00868.1|AF279143_1 tumor endothelial marker 2 [Homo sapiens]
1-278 MPASLALLQPRAMMKTLSSGNCTLSVPAKN
SYRMVVLGASRVGKSSIVSRFLNGRFEDQY
TPTIEDFHRKVYNIRGDMYQLDILDTSGNH
PFPAMRRLSILTGDVFILVFSLDNRESFDE
VKRLQKQILEVKSCLKNKTKEAAELPMVIC
GNKNDHGELCRQVPTTEAELLVSGDENCAY
FEVSAKKNTNVDEMFYVLFSMAKLPHEMSP
ALHRKISVQYGDAFHPRPFCMRRVKEMDAY
GMVSPFARRPSVNSDLKYIKAKVLREGQAR
ERDKCTIQ
low complexity regions: SEG 45 3.4 3.75
>gi|9857402|gb|AAG00868.1|AF279143_1 tumor endothelial marker 2 [Homo sapiens]
1-278 MPASLALLQPRAMMKTLSSGNCTLSVPAKN
SYRMVVLGASRVGKSSIVSRFLNGRFEDQY
TPTIEDFHRKVYNIRGDMYQLDILDTSGNH
PFPAMRRLSILTGDVFILVFSLDNRESFDE
VKRLQKQILEVKSCLKNKTKEAAELPMVIC
GNKNDHGELCRQVPTTEAELLVSGDENCAY
FEVSAKKNTNVDEMFYVLFSMAKLPHEMSP
ALHRKISVQYGDAFHPRPFCMRRVKEMDAY
GMVSPFARRPSVNSDLKYIKAKVLREGQAR
ERDKCTIQ
low complexity regions: XNU
# Score cutoff = 21, Search from offsets 1 to 4
# both members of each repeat flagged
# lambda = 0.347, K = 0.200, H = 0.664
>gi|9857402|gb|AAG00868.1|AF279143_1 tumor endothelial marker 2 [Homo sapiens]
MPASLALLQPRAMMKTLSSGNCTLSVPAKNSYRMVVLGASRVGKSSIVSRFLNGRFEDQY
TPTIEDFHRKVYNIRGDMYQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRESFDE
VKRLQKQILEVKSCLKNKTKEAAELPMVICGNKNDHGELCRQVPTTEAELLVSGDENCAY
FEVSAKKNTNVDEMFYVLFSMAKLPHEMSPALHRKISVQYGDAFHPRPFCMRRVKEMDAY
GMVSPFARRPSVNSDLKYIKAKVLREGQARERDKCTIQ
1 - 278 MPASLALLQP RAMMKTLSSG NCTLSVPAKN SYRMVVLGAS RVGKSSIVSR FLNGRFEDQY
TPTIEDFHRK VYNIRGDMYQ LDILDTSGNH PFPAMRRLSI LTGDVFILVF SLDNRESFDE
VKRLQKQILE VKSCLKNKTK EAAELPMVIC GNKNDHGELC RQVPTTEAEL LVSGDENCAY
FEVSAKKNTN VDEMFYVLFS MAKLPHEMSP ALHRKISVQY GDAFHPRPFC MRRVKEMDAY
GMVSPFARRP SVNSDLKYIK AKVLREGQAR ERDKCTIQ
low complexity regions: DUST
>gi|9857402|gb|AAG00868.1|AF279143_1 tumor endothelial marker 2 [Homo sapiens]
MPASLALLQPRAMMKTLSSGNCTLSVPAKNSYRMVVLGASRVGKSSIVSRFLNGRFEDQY
TPTIEDFHRKVYNIRGDMYQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRESFDE
VKRLQKQILEVKSCLKNKTKEAAELPMVICGNKNDHGELCRQVPTTEAELLVSGDENCAY
FEVSAKKNTNVDEMFYVLFSMAKLPHEMSPALHRKISVQYGDAFHPRPFCMRRVKEMDAY
GMVSPFARRPSVNSDLKYIKAKVLREGQARERDKCTIQ
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
coiled coil prediction for gi|9857402|gb|AAG00868.1|AF279143_1
sequence: 210 amino acids, 23 residue(s) in coiled coil state
Coil 1 * 118* 141* DEVKRLQKQILEVKSCLKNKTKEA
. | . | . | . | . | . 60
MPASLALLQP RAMMKTLSSG NCTLSVPAKN SYRMVVLGAS RVGKSSIVSR FLNGRFEDQY
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 120
TPTIEDFHRK VYNIRGDMYQ LDILDTSGNH PFPAMRRLSI LTGDVFILVF SLDNRESFDE
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~233333355 * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- -fgabcdefg * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~11111144 * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~133333333 * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~14444999 * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~12555599 * 14 M'95 -w local
. | . | . | . | . | . 180
VKRLQKQILE VKSCLKNKTK EAAELPMVIC GNKNDHGELC RQVPTTEAEL LVSGDENCAY
5555555555 5555555555 54444~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
abcdefgabc defgabcdef gabcd----- ---------- ---------- ---------- * 21 M'95 -w register
4444444444 4444444444 43333~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
3333333333 3331111111 11111~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
9999999999 9999999999 999993~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
9999999999 998~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . |
FEVSAKKNTN VDEMFYVLFS MAKLPHEMSP
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~
---------- ---------- ----------
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
prediction of transmembrane regions with toppred2
***********************************
*TOPPREDM with eukaryotic function*
***********************************
tem2.___inter___ is a single sequence
Using hydrophobicity file: /bio_software/2D/toppredm/lib/Engelman-Steitz.scale
Using cyt/ext file: /bio_software/2D/toppredm/lib/Cyt-Ext.prok
Using sequence file: tem2.___inter___
(1 sequences)
MPASLALLQPRAMMKTLSSGNCTLSVPAKNSYRMVVLGASRVGKSSIVSR
FLNGRFEDQYTPTIEDFHRKVYNIRGDMYQLDILDTSGNHPFPAMRRLSI
LTGDVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKEAAELPMVIC
GNKNDHGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTNVDEMFYVLFS
MAKLPHEMSPALHRKISVQYGDAFHPRPFCMRRVKEMDAYGMVSPFARRP
SVNSDLKYIKAKVLREGQARERDKCTIQ
(p)rokaryotic or (e)ukaryotic: e
Charge-pair energy: 0
Length of full window (odd number!): 21
Length of core window (odd number!): 11
Number of residues to add to each end of helix: 1
Critical length: 60
Upper cutoff for candidates: 1
Lower cutoff for candidates: 0.6
Total of 1 structures are to be tested
Candidate membrane-spanning segments:
Helix Begin End Score Certainity
----------------------------------------------------------------------
Structure 1
Transmembrane segments included in this structure:
Segment
Loop length 278
K+R profile +
CYT-EXT prof 0.48
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 0.00
Tm probability: 1.00
-> Orientation: undecided
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.1233
NEG: 32.0000
POS: 41.0000
-> Orientation: N-in
CYT-EXT difference: 0.48
-> Orientation: N-out
----------------------------------------------------------------------
"tem2" 278
************************************
*TOPPREDM with prokaryotic function*
************************************
tem2.___inter___ is a single sequence
Using hydrophobicity file: /bio_software/2D/toppredm/lib/Engelman-Steitz.scale
Using cyt/ext file: /bio_software/2D/toppredm/lib/Cyt-Ext.prok
Using sequence file: tem2.___inter___
(1 sequences)
MPASLALLQPRAMMKTLSSGNCTLSVPAKNSYRMVVLGASRVGKSSIVSR
FLNGRFEDQYTPTIEDFHRKVYNIRGDMYQLDILDTSGNHPFPAMRRLSI
LTGDVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKEAAELPMVIC
GNKNDHGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTNVDEMFYVLFS
MAKLPHEMSPALHRKISVQYGDAFHPRPFCMRRVKEMDAYGMVSPFARRP
SVNSDLKYIKAKVLREGQARERDKCTIQ
(p)rokaryotic or (e)ukaryotic: p
Charge-pair energy: 0
Length of full window (odd number!): 21
Length of core window (odd number!): 11
Number of residues to add to each end of helix: 1
Critical length: 60
Upper cutoff for candidates: 1
Lower cutoff for candidates: 0.6
Total of 1 structures are to be tested
Candidate membrane-spanning segments:
Helix Begin End Score Certainity
----------------------------------------------------------------------
Structure 1
Transmembrane segments included in this structure:
Segment
Loop length 278
K+R profile +
CYT-EXT prof 0.48
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 0.00
Tm probability: 1.00
-> Orientation: undecided
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.1233
NEG: 32.0000
POS: 41.0000
-> Orientation: N-in
CYT-EXT difference: 0.48
-> Orientation: N-out
----------------------------------------------------------------------
"tem2" 278
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
NOW EXECUTING: /bio_software/1D/stat/saps/saps-stroh/SAPS.SSPA/saps /people/maria/tem2.___saps___
SAPS. Version of April 11, 1996.
Date run: Fri Oct 13 19:37:29 2000
File: /people/maria/tem2.___saps___
ID gi|9857402|gb|AAG00868.1|AF279143_1
DE tumor endothelial marker 2 [Homo sapiens]
number of residues: 278; molecular weight: 31.6 kdal
1 MPASLALLQP RAMMKTLSSG NCTLSVPAKN SYRMVVLGAS RVGKSSIVSR FLNGRFEDQY
61 TPTIEDFHRK VYNIRGDMYQ LDILDTSGNH PFPAMRRLSI LTGDVFILVF SLDNRESFDE
121 VKRLQKQILE VKSCLKNKTK EAAELPMVIC GNKNDHGELC RQVPTTEAEL LVSGDENCAY
181 FEVSAKKNTN VDEMFYVLFS MAKLPHEMSP ALHRKISVQY GDAFHPRPFC MRRVKEMDAY
241 GMVSPFARRP SVNSDLKYIK AKVLREGQAR ERDKCTIQ
--------------------------------------------------------------------------------
COMPOSITIONAL ANALYSIS (extremes relative to: swp23s)
A : 18( 6.5%); C : 7( 2.5%); D : 15( 5.4%); E : 17( 6.1%); F : 13( 4.7%)
G : 13( 4.7%); H : 6( 2.2%); I : 11( 4.0%); K : 20( 7.2%); L : 25( 9.0%)
M+ : 13( 4.7%); N : 13( 4.7%); P : 14( 5.0%); Q : 9( 3.2%); R : 21( 7.6%)
S : 22( 7.9%); T : 11( 4.0%); V : 21( 7.6%); W : 0( 0.0%); Y : 9( 3.2%)
KR : 41 ( 14.7%); ED : 32 ( 11.5%); AGP : 45 ( 16.2%);
KRED : 73 ( 26.3%); KR-ED : 9 ( 3.2%); FIKMNY : 79 ( 28.4%);
LVIFM : 83 ( 29.9%); ST : 33 ( 11.9%).
--------------------------------------------------------------------------------
CHARGE DISTRIBUTIONAL ANALYSIS
1 0000000000 +000+00000 00000000+0 00+0000000 +00+00000+ 0000+0--00
61 0000--00++ 0000+0-000 0-00-00000 00000++000 000-000000 00-0+-00--
121 0++00+000- 0+000+0+0+ -00-000000 00+0-00-00 +00000-0-0 0000--0000
181 0-000++000 0--0000000 00+000-000 000++00000 0-0000+000 0++0+-0-00
241 0000000++0 0000-0+00+ 0+00+-000+ -+-+0000
A. CHARGE CLUSTERS.
Positive charge clusters (cmin = 12/30 or 16/45 or 19/60): none
Negative charge clusters (cmin = 10/30 or 13/45 or 16/60): none
Mixed charge clusters (cmin = 17/30 or 23/45 or 29/60): none
B. HIGH SCORING (UN)CHARGED SEGMENTS.
There are no high scoring positive charge segments.
There are no high scoring negative charge segments.
There are no high scoring mixed charge segments.
There are no high scoring uncharged segments.
C. CHARGE RUNS AND PATTERNS.
pattern (+)| (-)| (*)| (0)| (+0)| (-0)| (*0)|(+00)|(-00)|(*00)| (H.)|(H..)|
lmin0 5 | 5 | 7 | 29 | 10 | 9 | 13 | 11 | 10 | 15 | 6 | 8 |
lmin1 7 | 6 | 9 | 35 | 12 | 11 | 16 | 14 | 13 | 18 | 8 | 10 |
lmin2 8 | 7 | 11 | 39 | 13 | 12 | 17 | 16 | 14 | 20 | 9 | 11 |
(Significance level: 0.010000; Minimal displayed length: 6)
There are no charge runs or patterns exceeding the given minimal lengths.
Run count statistics:
+ runs >= 4: 0
- runs >= 3: 0
* runs >= 5: 1, at 270;
0 runs >= 19: 0
--------------------------------------------------------------------------------
DISTRIBUTION OF OTHER AMINO ACID TYPES
1. HIGH SCORING SEGMENTS.
There are no high scoring hydrophobic segments.
There are no high scoring transmembrane segments.
2. SPACINGS OF C.
H2N-21-C-111-C-15-C-9-C-17-C-51-C-44-C-3-COOH
2*. SPACINGS OF C and H. (additional deluxe function for ALEX)
H2N-21-C-45-H-21-H-43-C-15-C-5-H-3-C-17-C-27-H-6-H-11-H-4-C-44-C-3-COOH
--------------------------------------------------------------------------------
REPETITIVE STRUCTURES.
A. SEPARATED, TANDEM, AND PERIODIC REPEATS: amino acid alphabet.
Repeat core block length: 4
B. SEPARATED AND TANDEM REPEATS: 11-letter reduced alphabet.
(i= LVIF; += KR; -= ED; s= AG; o= ST; n= NQ; a= YW; p= P; h= H; m= M; c= C)
Repeat core block length: 8
--------------------------------------------------------------------------------
MULTIPLETS.
A. AMINO ACID ALPHABET.
1. Total number of amino acid multiplets: 12 (Expected range: 3-- 27)
2. Histogram of spacings between consecutive amino acid multiplets:
(1-5) 3 (6-10) 2 (11-20) 3 (>=21) 5
3. Clusters of amino acid multiplets (cmin = 9/30 or 11/45 or 13/60): none
B. CHARGE ALPHABET.
1. Total number of
charge multiplets: 12 (Expected range: 0-- 17)
7 +plets (f+: 14.7%), 5 -plets (f-: 11.5%)
Total number of charge altplets: 5 (Critical number: 20)
2. Histogram of spacings between consecutive charge multiplets:
(1-5) 3 (6-10) 2 (11-20) 2 (>=21) 6
--------------------------------------------------------------------------------
PERIODICITY ANALYSIS.
A. AMINO ACID ALPHABET (core: 4; !-core: 5)
Location Period Element Copies Core Errors
There are no periodicities of the prescribed length.
B. CHARGE ALPHABET ({+= KR; -= ED; 0}; core: 5; !-core: 6)
and HYDROPHOBICITY ALPHABET ({*= KRED; i= LVIF; 0}; core: 6; !-core: 9)
Location Period Element Copies Core Errors
105- 110 1 i 6 6 0
--------------------------------------------------------------------------------
SPACING ANALYSIS.
There are no unusual spacings.
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
Start with Pfam (from /data/patterns/pfam)
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file: /data/patterns/pfam/Pfam
Sequence file: tem2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: gi|9857402|gb|AAG00868.1|AF279143_1 tumor endothelial marker 2 [Homo sapiens]
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
ras Ras family 119.7 5.6e-32 1
Flu_M1 Influenza Matrix protein (M1) -1.2 99 1
arf ADP-ribosylation factor family -106.3 0.76 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
Flu_M1 1/1 34 52 .. 226 244 .] -1.2 99
arf 1/1 14 205 .. 1 191 [] -106.3 0.76
ras 1/1 33 278 .] 1 198 [] 119.7 5.6e-32
Alignments of top-scoring domains:
Flu_M1: domain 1 of 1, from 34 to 52: score -1.2, E = 99
*->lEvLkayskrnsvlvkkyk<-*
+ vL a+ + s v +++
gi|9857402 34 MVVLGASRVGKSSIVSRFL 52
arf: domain 1 of 1, from 14 to 205: score -106.3, E = 0.76
*->GlgflkiflkllglwnKEmRILiLGLDnAGKTTILykLklgei.vtt
+ ++++ l + + ++R+++LG GK+ I+ ++ +g+++++
gi|9857402 14 MKTLSSGNCTLSVPAKNSYRMVVLGASRVGKSSIVSRFLNGRFeDQY 60
iPTi...GFNvEtveykNikFtvWDvGGQe...slRPlWrnYfpnTdavI
+PTi++ + v + + + + D G ++ + R+l + ++ +++
gi|9857402 61 TPTIedfHRKVYNIRGDMYQLDILDTSGNHpfpAMRRL-SILTGDVFILV 109
fVVDSaDreRieeakeELhaLLneeeL........adAplLifANKQDlp
f D re ++e k+ + +L+ + ++++++ a+ p +i +NK D
gi|9857402 110 FSLD--NRESFDEVKRLQKQILEVKSClknktkeaAELPMVICGNKNDHG 157
gAmseaEireaLgLhelkgskgkVtlegdRpWeiqgcsAvkGeGLyEGld
+ +ea el s gd + + sA k ++++E +
gi|9857402 158 ELCRQVPTTEA----ELLVS-------GDENCAYFEVSAKKNTNVDEMFY 196
WLsnnikkr<-*
L + k +
gi|9857402 197 VLFSMAKLP 205
ras: domain 1 of 1, from 33 to 278: score 119.7, E = 5.6e-32
*->KlVliGDsGVGKSsLliRFtdnkFveeYipTIGvDFytktvevDGkt
V++G s VGKSs++ RF +++F ++Y pTI +DF++k ++ G
gi|9857402 33 RMVVLGASRVGKSSIVSRFLNGRFEDQYTPTI-EDFHRKVYNIRGDM 78
vkLqIWDTAGQERFralrpaYYRgAqGfLLVYDITsrdSFenvkkWleei
+ L+I+DT G F a+r+++ ++++f+LV++ ++r+SF++vk+ ++i
gi|9857402 79 YQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQI 128
lrhad........kdenvpivLVGNKcDLeddedleltegqkRvVsteeG
l++++ ++++++ e p+v++GNK D + R+V+t e+
gi|9857402 129 LEVKSclknktkeAAE-LPMVICGNKNDHGE--------LC-RQVPTTEA 168
eal.AkelgAlpFmETSAKtntNVeeaFeelareilkkvse.........
e l + + + ++E SAK ntNV+e F+ l ++ + + ++ +++ +
gi|9857402 169 ELLvSGDEN-CAYFEVSAKKNTNVDEMFYVLFSMAKLPHEMspalhrkis 217
............................................vnvnld
+ ++ ++++ ++ ++ + + ++ ++++ +++ + + ++++
gi|9857402 218 vqygdafhprpfcmrrvkemdaygmvspfarrpsvnsdlkyikaKVLREG 267
qpakkkkskCcil<-*
q + + kC i
gi|9857402 268 QARERD--KCTIQ 278
//
Start with PfamFrag (from /data/patterns/pfam)
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file: /data/patterns/pfam/PfamFrag
Sequence file: tem2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: gi|9857402|gb|AAG00868.1|AF279143_1 tumor endothelial marker 2 [Homo sapiens]
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
ras Ras family 130.0 2e-37 1
ELH Egg-laying hormone precursor 1.7 5.1 1
Flu_M1 Influenza Matrix protein (M1) -1.2 99 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
Flu_M1 1/1 34 52 .. 226 244 .] -1.2 99
ras 1/1 33 198 .. 1 169 [. 130.0 2e-37
ELH 1/1 244 275 .. 200 230 .. 1.7 5.1
Alignments of top-scoring domains:
Flu_M1: domain 1 of 1, from 34 to 52: score -1.2, E = 99
*->lEvLkayskrnsvlvkkyk<-*
+ vL a+ + s v +++
gi|9857402 34 MVVLGASRVGKSSIVSRFL 52
ras: domain 1 of 1, from 33 to 198: score 130.0, E = 2e-37
*->KlVliGDsGVGKSsLliRFtdnkFveeYipTIGvDFytktvevDGkt
V++G s VGKSs++ RF +++F ++Y pTI +DF++k ++ G
gi|9857402 33 RMVVLGASRVGKSSIVSRFLNGRFEDQYTPTI-EDFHRKVYNIRGDM 78
vkLqIWDTAGQERFralrpaYYRgAqGfLLVYDITsrdSFenvkkWleei
+ L+I+DT G F a+r+++ ++++f+LV++ ++r+SF++vk+ ++i
gi|9857402 79 YQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQI 128
lrhad........kdenvpivLVGNKcDLeddedleltegqkRvVsteeG
l++++ ++++++ e p+v++GNK D + R+V+t e+
gi|9857402 129 LEVKSclknktkeAAE-LPMVICGNKNDHGE--------LC-RQVPTTEA 168
eal.AkelgAlpFmETSAKtntNVeeaFeel<-*
e l + + + ++E SAK ntNV+e F+ l
gi|9857402 169 ELLvSGDEN-CAYFEVSAKKNTNVDEMFYVL 198
ELH: domain 1 of 1, from 244 to 275: score 1.7, E = 5.1
*->sGisKRisinqDLKAitDmLltE.qiqeRery<-*
s ++R s+n DLK i +l E+q +eR ++
gi|9857402 244 SPFARRPSVNSDLKYIKAKVLREgQARERDKC 275
//
Start with Repeat Library (from /data/patterns/repeats-Miguel-Andrade/hmm)
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file: /data/patterns/repeats-Miguel-Andrade/hmm/repeats.hmm-lib
Sequence file: tem2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: gi|9857402|gb|AAG00868.1|AF279143_1 tumor endothelial marker 2 [Homo sapiens]
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
[no hits above thresholds]
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
[no hits above thresholds]
Alignments of top-scoring domains:
[no hits above thresholds]
//
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
Start with Prosite
---------------------------------------------------------
| ppsearch (c) 1994 EMBL Data Library |
| based on MacPattern (c) 1990-1994 R. Fuchs |
---------------------------------------------------------
PROSITE pattern search started: Fri Oct 13 19:39:03 2000
Sequence file: tem2
----------------------------------------
Sequence gi|9857402|gb|AAG00868.1|AF279143_1 (278 residues):
Matching pattern PS00001 ASN_GLYCOSYLATION:
21: NCTL
137: NKTK
Total matches: 2
Matching pattern PS00004 CAMP_PHOSPHO_SITE:
96: RRLS
186: KKNT
214: RKIS
248: RRPS
Total matches: 4
Matching pattern PS00005 PKC_PHOSPHO_SITE:
31: SYR
184: SAK
Total matches: 2
Matching pattern PS00006 CK2_PHOSPHO_SITE:
63: TIED
117: SFDE
166: TEAE
173: SGDE
189: TNVD
Total matches: 5
Matching pattern PS00007 TYR_PHOSPHO_SITE:
232: RRVKEMDAY
Total matches: 1
Matching pattern PS00294 PRENYLATION:
275: CTIQ
Total matches: 1
Matching pattern PS00016 RGD:
75: RGD
Total matches: 1
Matching pattern PS00017 ATP_GTP_A:
38: GASRVGKS
Total matches: 1
Total no of hits in this sequence: 17
========================================
1314 pattern(s) searched in 1 sequence(s), 278 residues.
Total no of hits in all sequences: 17.
Search time: 00:00 min
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
Start with Profile Search
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
Start with motif search against own library
***** bioMotif : Version V41a DB, 1999 Nov 11 *****
argv[1]=P
argv[2]=-m /data/patterns/own/motif.fa
argv[4]=-seq tem2
***** bioMotif : Version V41a DB, 1999 Nov 11 *****
SeqTyp=2 : PROTEIN search;
>APC D-Box is the MOTIF name
>STATISTICS Total : 0 solutions in 0 sequences, 0 units; out of 1 sequences, 278 units
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~ ~~~
Start with HMM-search search against own library
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file: /data/patterns/own/own-hmm.lib
Sequence file: tem2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: gi|9857402|gb|AAG00868.1|AF279143_1 tumor endothelial marker 2 [Homo sapiens]
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
[no hits above thresholds]
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
[no hits above thresholds]
Alignments of top-scoring domains:
[no hits above thresholds]
//
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file: /data/patterns/own/own-hmm-f.lib
Sequence file: tem2
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: gi|9857402|gb|AAG00868.1|AF279143_1 tumor endothelial marker 2 [Homo sapiens]
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
[no hits above thresholds]
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
[no hits above thresholds]
Alignments of top-scoring domains:
[no hits above thresholds]
//
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
L. Aravind's signalling DB
IMPALA version 1.1 [20-December-1999]
Reference: Alejandro A. Schaffer, Yuri I. Wolf, Chris P. Ponting,
Eugene V. Koonin, L. Aravind, Stephen F. Altschul (1999),
"IMPALA: Matching a Protein Sequence Against a Collection of
"PSI-BLAST-Constructed Position-Specific Score Matrices",
Bioinformatics 15:1000-1011.
Query= gi|9857402|gb|AAG00868.1|AF279143_1 tumor endothelial marker 2
[Homo sapiens]
(278 letters)
Searching..................................done
Results from profile search
Score E
Sequences producing significant alignments: (bits) Value
SGTP Small GTPAses 151 8e-40
CATH Cathepsin like protease domain 26 0.083
LAMNT Lamin N-terminal domain 25 0.10
14-3-3 14-3-3 protein alpha Helical domain 21 1.6
RASGAP RAS-type GTPase GTP hydrolysis activating protein 21 1.7
CBS cystathionine beta -synthase domain (A predicted ligand... 21 2.3
CALC Calcineurin like Phosphoesterase domain 20 5.2
PDE cyclic NMP phosphodiesterase domain 19 7.5
LRR Leucine rich repeats 19 9.2
RHOGEF RHO-type GTPase GDP exchange factor 19 9.7
>SGTP Small GTPAses
Length = 164
Score = 151 bits (380), Expect = 8e-40
Identities = 40/168 (23%), Positives = 77/168 (45%), Gaps = 12/168 (7%)
Query: 32 YRMVVLGASRVGKSSIVSRFLNGRFEDQYTPTIE-DFHRKVYNIRGDMYQLDILDTSGNH 90
++ +V+G + GKS ++ +F+ +F+D TI +F K+ N+ G +L I DT+G
Sbjct: 1 FKFLVIGNAGTGKSCLLHQFIEKKFKDDSNHTIGVEFGSKIINVGGKYVKLQIWDTAGQE 60
Query: 91 PFPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKEAAELPMVIC 150
F ++ R +LV+ + +RE+++ + + + + +++C
Sbjct: 61 RFRSVTRSYYRGAAGALLVYDITSRETYNALTNWLTDA---------RMLASQNIVIILC 111
Query: 151 GNKNDHGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTNVDEMFYVL 198
GNK D R+V EA + + E SA +V+E F
Sbjct: 112 GNKKDLDA-DREVTFLEASRFAQ-ENELMFLETSALTGEDVEEAFVQC 157
>CATH Cathepsin like protease domain
Length = 371
Score = 25.7 bits (56), Expect = 0.083
Identities = 18/69 (26%), Positives = 23/69 (33%), Gaps = 15/69 (21%)
Query: 62 PTIE-DFHRKVYNIRGDMYQLDILDTSGNH---PFPAMR------RLSILTGDVFI---- 107
P + K Y + + Y L + F + L IL GDVFI
Sbjct: 294 PNVNLVLGGKSYALTPNQYVLKVTVQGETLCLSGFSGLDVPPPAGPLWIL-GDVFIGSYY 352
Query: 108 LVFSLDNRE 116
VF DN
Sbjct: 353 TVFDRDNDA 361
>LAMNT Lamin N-terminal domain
Length = 256
Score = 25.3 bits (55), Expect = 0.10
Identities = 20/79 (25%), Positives = 28/79 (35%), Gaps = 5/79 (6%)
Query: 193 EMFYVLFSMAKLPHEMSPALHRKISVQYGDAFHP-RPFCMRRVKEMDAYGMVS--PFARR 249
+ YV MA P S L R S +G + P F + M +GM S P +
Sbjct: 127 HVAYVWIQMANSPRPGSWVLER--STDHGKTYQPWFNFAENAAECMRRFGMESLSPISED 184
Query: 250 PSVNSDLKYIKAKVLREGQ 268
SV + L +
Sbjct: 185 DSVTCRTDMASLQPLENAE 203
Score = 19.5 bits (40), Expect = 6.1
Identities = 7/32 (21%), Positives = 15/32 (46%), Gaps = 4/32 (12%)
Query: 47 IVSRFLNGRF---EDQYTPTIEDFHRKVYNIR 75
+V R L R + + +++F + N+R
Sbjct: 204 MVIRILEHRPSSRQFATSEALQNF-TRATNVR 234
>14-3-3 14-3-3 protein alpha Helical domain
Length = 270
Score = 21.5 bits (45), Expect = 1.6
Identities = 10/45 (22%), Positives = 19/45 (42%)
Query: 176 ENCAYFEVSAKKNTNVDEMFYVLFSMAKLPHEMSPALHRKISVQY 220
E+ Y A++ + M + S+A E++ +SV Y
Sbjct: 7 EDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAY 51
>RASGAP RAS-type GTPase GTP hydrolysis activating protein
Length = 292
Score = 21.3 bits (44), Expect = 1.7
Identities = 11/32 (34%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Query: 82 DILDTSGNHPFPAMR--RLSILTGDVFILVFS 111
D+ + +G H FP+ R R S+++G +F+ F+
Sbjct: 182 DLRECAGEH-FPSNREVRYSVVSGFIFLRFFA 212
>CBS cystathionine beta -synthase domain (A predicted ligand binding domain)
Length = 214
Score = 20.7 bits (43), Expect = 2.3
Identities = 5/43 (11%), Positives = 13/43 (29%)
Query: 48 VSRFLNGRFEDQYTPTIEDFHRKVYNIRGDMYQLDILDTSGNH 90
V + L +E ++ H + +I + +
Sbjct: 117 VVKTLAKLYEIPVYKIFKELHNHIGDISWEELMEAAAVVTKRM 159
>CALC Calcineurin like Phosphoesterase domain
Length = 274
Score = 19.8 bits (40), Expect = 5.2
Identities = 11/65 (16%), Positives = 21/65 (31%)
Query: 28 AKNSYRMVVLGASRVGKSSIVSRFLNGRFEDQYTPTIEDFHRKVYNIRGDMYQLDILDTS 87
Y +VVL + +S N R + + F + G ++Q + +
Sbjct: 153 NPERYTLVVLHHHLLPTNSAWLDQHNLRNSHELAEVLAPFTNVKAILYGHIHQEVNSEWN 212
Query: 88 GNHPF 92
G
Sbjct: 213 GYQVM 217
>PDE cyclic NMP phosphodiesterase domain
Length = 350
Score = 18.9 bits (38), Expect = 7.5
Identities = 13/46 (28%), Positives = 21/46 (45%), Gaps = 2/46 (4%)
Query: 82 DILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQ 127
D +D + F +M + L F+L NRE++ LQK+
Sbjct: 272 DFIDFVASPLFQSMAK--FLKESQFLLKVISKNRENWQAYMELQKE 315
>LRR Leucine rich repeats
Length = 339
Score = 18.8 bits (38), Expect = 9.2
Identities = 10/50 (20%), Positives = 19/50 (38%), Gaps = 12/50 (24%)
Query: 76 GDMYQLDILDTSGNH-------PFPAMRRLSILTGDVFILVFSLDNRESF 118
+ L +LD N P A ++L+ + FSL+++
Sbjct: 289 HLLPDLKVLDLHENENLVMPPKPNDARKKLAFYN-----IDFSLEHQRKI 333
>RHOGEF RHO-type GTPase GDP exchange factor
Length = 176
Score = 18.7 bits (38), Expect = 9.7
Identities = 7/24 (29%), Positives = 9/24 (37%)
Query: 112 LDNRESFDEVKRLQKQILEVKSCL 135
F E +R K L + S L
Sbjct: 107 YSECAFFQECQRKLKHRLRLDSYL 130
Underlying Matrix: BLOSUM62
Number of sequences tested against query: 105
Number of sequences better than 10.0: 10
Number of calls to ALIGN: 11
Length of query: 278
Total length of test sequences: 20182
Effective length of test sequences: 16536.0
Effective search space size: 4025149.7
Initial X dropoff for ALIGN: 25.0 bits
Y. Wolf's SCOP PSSM
IMPALA version 1.1 [20-December-1999]
Reference: Alejandro A. Schaffer, Yuri I. Wolf, Chris P. Ponting,
Eugene V. Koonin, L. Aravind, Stephen F. Altschul (1999),
"IMPALA: Matching a Protein Sequence Against a Collection of
"PSI-BLAST-Constructed Position-Specific Score Matrices",
Bioinformatics 15:1000-1011.
Query= gi|9857402|gb|AAG00868.1|AF279143_1 tumor endothelial marker 2
[Homo sapiens]
(278 letters)
Searching.................................................done
Results from profile search
Score E
Sequences producing significant alignments: (bits) Value
gi|1572819 [7..164] P-loop containing nucleotide triphosphat... 104 2e-24
gi|1531539 [70..226] P-loop containing nucleotide triphospha... 98 1e-22
gi|1086887 [26..189] P-loop containing nucleotide triphospha... 98 2e-22
gi|1666073 [6..176] P-loop containing nucleotide triphosphat... 95 8e-22
gi|4006 [276..439] P-loop containing nucleotide triphosphate... 90 3e-20
gi|2814284 [1..224] P-loop containing nucleotide triphosphat... 90 6e-20
gi|1301656 [50..208] P-loop containing nucleotide triphospha... 88 2e-19
gi|2132165 [3..190] P-loop containing nucleotide triphosphat... 86 9e-19
gi|1695241 [169..336] P-loop containing nucleotide triphosph... 82 1e-17
gi|232142 [190..392] P-loop containing nucleotide triphospha... 79 6e-17
gi|729400 [38..325] P-loop containing nucleotide triphosphat... 73 7e-15
gi|2136468 [19..195] Lipocalins 31 0.025
gi|1515341 [77..406] Acid proteases 28 0.13
gi|2496715 [53..359] NAD(P)-binding Rossmann-fold domains 25 1.2
gi|2144317 [224..416] Lactate & malate dehydrogenases, C-ter... 25 1.7
gi|417211 [2..187] P-loop containing nucleotide triphosphate... 25 1.8
gi|2652959 [169..353] P-loop containing nucleotide triphosph... 23 4.2
gi|1175188 [41..508] Periplasmic binding protein-like II 23 4.4
gi|1723793 [7..233] NAD(P)-binding Rossmann-fold domains 23 5.7
gi|345362 [61..347] Protein kinases (PK), catalytic core 23 5.7
gi|1174715 [19..356] Thiamin-binding 23 5.8
gi|134579 [59..285] P-loop containing nucleotide triphosphat... 23 5.9
gi|120586 [1..210] Class II aldolase 23 6.3
gi|2392193 [1..232] Zn metallo-beta-lactamase 23 6.7
gi|2493523 [117..286] Interleukin-1beta converting enzyme (a... 23 7.0
gi|127802 [1..266] Glucosamine 6-phosphate deaminase 23 7.1
gi|417569 [3..336] Aspartate/ornithine carbamoyltransferase,... 23 7.4
gi|1346784 [8..238] N-terminal nucleophile aminohydrolases (... 22 8.2
gi|280504 [161..330] Glyceraldehyde-3-phosphate dehydrogenas... 22 9.2
gi|1515148 [263..496] Protein kinases (PK), catalytic core 22 9.2
gi|2130121 [317..446] Cyclin-like 23 9.3
gi|1673470 [12..233] Protein kinases (PK), catalytic core 22 9.6
gi|1322391 [16..428] Acid proteases 22 9.9
>gi|1572819 [7..164] P-loop containing nucleotide triphosphate hydrolases
Length = 158
Score = 104 bits (258), Expect = 2e-24
Identities = 33/165 (20%), Positives = 64/165 (38%), Gaps = 10/165 (6%)
Query: 32 YRMVVLGASRVGKSSIVSRFLNGRFEDQYTPTIEDFHRKVYNIRGDMYQLDILDTSGNHP 91
R+V++G GK+S+V L + D + D ++ + I+D S
Sbjct: 4 VRIVLIGDEGCGKTSLVMSLLEDEWVDAVPRRL-DRVLIPADVTPENVTTSIVDLSIKEE 62
Query: 92 FPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKEAAELPMVICG 151
I +V +V+S+ + + D ++ ++ ++T P+++ G
Sbjct: 63 DENWIVSEIRQANVICVVYSVTDESTVDGIQTKWLPLIRQSFGEYHET------PVILVG 116
Query: 152 NKNDHGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTNVDEMFY 196
NK+D + + E E SA+ NV E+FY
Sbjct: 117 NKSDGTA--NNTDKILPIMEAN-TEVETCVECSARTMKNVSEIFY 158
>gi|1531539 [70..226] P-loop containing nucleotide triphosphate hydrolases
Length = 157
Score = 98.1 bits (241), Expect = 1e-22
Identities = 44/168 (26%), Positives = 73/168 (43%), Gaps = 13/168 (7%)
Query: 32 YRMVVLGASRVGKSSIVSRFLNGRFEDQYTPTIEDFHRKVYNIRGDMYQLDILDTSGNHP 91
R+ VLG +R GKSS++ RFL G ++ T + ++K + G + + I +
Sbjct: 2 LRLGVLGDARSGKSSLIHRFLTGSYQVLE-KTESEQYKKEMLVDGQTHLVLIREE----- 55
Query: 92 FPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKEAAELPMVICG 151
A D I VFSL++ SF V RL Q+ ++ + L + + G
Sbjct: 56 AGAPDAKFSGWADAVIFVFSLEDENSFQAVSRLHGQLSSLRG------EGRGGLALALVG 109
Query: 152 NKND-HGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTNVDEMFYVL 198
++ R V A L + + C+Y+E A NVD +F +
Sbjct: 110 TQDRISASSPRVVGDARARALCADMKRCSYYETCATYGLNVDRVFQEV 157
>gi|1086887 [26..189] P-loop containing nucleotide triphosphate hydrolases
Length = 164
Score = 98.1 bits (241), Expect = 2e-22
Identities = 33/175 (18%), Positives = 66/175 (36%), Gaps = 14/175 (8%)
Query: 33 RMVVLGASRVGKSSIVSRFLNGRFEDQYTPTI-EDFHRKVYNIRGDMYQLDILDTSGNHP 91
+++V+GA GKS+ + R G+F ++ K + +L D G
Sbjct: 1 KIIVVGAKNAGKSTFIERVEFGKFHEEKQTEKLYRVIAKKVFEQTLTVELIEKDLGGLTN 60
Query: 92 -FPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKEAAELPMVIC 150
++ + D +L ++ D+ ESF ++K + + +
Sbjct: 61 EDNGFKKTEMRDVDAVLLFYAADDLESFKQLKENLVHVQRKIP---------PNANITVV 111
Query: 151 GNKNDHGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTNVDEMFYVLFSMAKLP 205
G K D E QV E + + ++ + FE S+K NV+ + + +
Sbjct: 112 GTKADVKE--MQVQWQEVDSF-AENQGFSCFETSSKTGVNVEIILHDILETIFER 163
>gi|1666073 [6..176] P-loop containing nucleotide triphosphate hydrolases
Length = 171
Score = 95.5 bits (234), Expect = 8e-22
Identities = 53/176 (30%), Positives = 81/176 (45%), Gaps = 17/176 (9%)
Query: 33 RMVVLGASRVGKSSIVSRFLNGRFEDQYTPTIEDFHRK----------VYNIRGDMYQLD 82
R+ VLGA VGK++I+ +FL G + +++ PT + +IR
Sbjct: 1 RVAVLGAPGVGKTAIIRQFLFGDYPERHRPTDGPRLYRPAVLLDGAVYDLSIRDGDVAGP 60
Query: 83 ILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKEA 142
G +P + S+ D F+LV+ + + +SFD VK L+++I E + A
Sbjct: 61 GSSPGGPEEWPDAKDWSLQDTDAFVLVYDICSPDSFDYVKALRQRIAETRP------AGA 114
Query: 143 AELPMVICGNKNDHGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTNVDEMFYVL 198
E P+++ GNK D L R P LV C Y E SAK N +V +F L
Sbjct: 115 PEAPILVVGNKRDRQRL-RFGPRRALAALVRRGWRCGYLECSAKYNWHVLRLFREL 169
>gi|4006 [276..439] P-loop containing nucleotide triphosphate hydrolases
Length = 164
Score = 90.4 bits (221), Expect = 3e-20
Identities = 31/167 (18%), Positives = 57/167 (33%), Gaps = 4/167 (2%)
Query: 33 RMVVLGASRVGKSSIVSRFLNGRFEDQYTPTIEDFHRKVYNIRGDMYQLDILDTSGNHPF 92
++V+LGA VGKSS+V+ N I + Y++ I DT+G
Sbjct: 1 KLVLLGAPNVGKSSLVNSLTNDDISIVSDIPGTTRDSIDAMINVNGYKVIICDTAGIREK 60
Query: 93 PAMRRLSILTGDVFILVFSLDNRE-SFDEVKRLQKQILEVKSCLKNKTKEAAELPMVICG 151
+ + + D D + ++ + L +KT ++I
Sbjct: 61 SSDKIEMLGIDRAKKKSVQSDLCLFIVDPTDLSKLLPEDILAHLSSKTFG--NKRIIIVV 118
Query: 152 NKNDHGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTNVDEMFYVL 198
NK+D ++ +L VS K ++ + L
Sbjct: 119 NKSDLVS-DDEMTKVLNKLQTRLGSKYPILSVSCKTKEGIESLISTL 164
>gi|2814284 [1..224] P-loop containing nucleotide triphosphate hydrolases
Length = 224
Score = 89.6 bits (219), Expect = 6e-20
Identities = 32/183 (17%), Positives = 64/183 (34%), Gaps = 22/183 (12%)
Query: 29 KNSYRMVVLGASRVGKSSIVSRFLNGRFEDQY--TPTIEDFHRKVYNIRGDMYQLDILDT 86
+ + + + + LN F+ + + + D + + G L + DT
Sbjct: 49 RKNLKKNLKKNLSILFYLKNHEKLNSFFKKRQLLSVNVFDTYSTNVMVDGRPINLSLWDT 108
Query: 87 SGNHPFPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKEAAELP 146
+G + R LS DVF++ F+L+N SF+ V+ + P
Sbjct: 109 AGQDDYDQFRHLSFPQTDVFLVCFALNNPASFENVRAKWYPEVSHHC---------PNTP 159
Query: 147 MVICGNKND-----------HGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTNVDEMF 195
+++ G K D + V T+ ++ + Y E SA + ++F
Sbjct: 160 IILVGTKADLREDRDTIERLRERRLQPVSHTQGYVMAKEIKAVKYLECSALTQIGLKQVF 219
Query: 196 YVL 198
Sbjct: 220 DEA 222
>gi|1301656 [50..208] P-loop containing nucleotide triphosphate hydrolases
Length = 159
Score = 87.6 bits (214), Expect = 2e-19
Identities = 37/167 (22%), Positives = 69/167 (41%), Gaps = 15/167 (8%)
Query: 32 YRMVVLGASRVGKSSIVSRFLNGRF---EDQYTPTIEDFHRKVYNIRGDMYQLDILDTSG 88
R+VV+G +VGK++I+ + + + + + L + DT+G
Sbjct: 3 MRVVVVGGKKVGKTAILRQVACVEDVTNKPYEPTIEDTYQVLLEEPDKAREILILHDTAG 62
Query: 89 NHPFPA--MRRLSILTGDVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKEAAELP 146
+ +++ + D F+LV+S + ESF+ V L+K I K + P
Sbjct: 63 VSNYGPIELKKAYVQAADAFVLVYSSADYESFNRVDLLKKWIDRQFGKDKKEV------P 116
Query: 147 MVICGNKNDHGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTNVDE 193
+V+ N D V + A + E FEV+AK ++ +
Sbjct: 117 IVVLANMRD---RPATVDSAFAHSWAA-REKVKLFEVTAKDRQSLVD 159
>gi|2132165 [3..190] P-loop containing nucleotide triphosphate hydrolases
Length = 188
Score = 85.7 bits (209), Expect = 9e-19
Identities = 22/176 (12%), Positives = 44/176 (24%), Gaps = 14/176 (7%)
Query: 29 KNSYRMVVLGASRVGKSSIVSRFLNGRFEDQYTPTIE---DFHRKVYNIRGDMYQLDILD 85
K Y +++LG GK++ + L D
Sbjct: 13 KEQYSILILGLDNAGKTTFLETLKKEYSLAFKALEKIQPTVGQNVATIPVDSKQILKFWD 72
Query: 86 TSGNHPFPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKEAAEL 145
G +M I + +RE DE + ++ + +
Sbjct: 73 VGGQESLRSMWSEYYSLCHGIIFIVDSSDRERLDECSTTLQSVVMDEEI--------EGV 124
Query: 146 PMVICGNKNDHGELCR---QVPTTEAELLVSGDENCAYFEVSAKKNTNVDEMFYVL 198
P+++ NK D + + +SA V + +
Sbjct: 125 PILMLANKQDRQDRMEVQDIKEVFNKIAEHISARDSRVLPISALTGEGVKDAIEWM 180
>gi|1695241 [169..336] P-loop containing nucleotide triphosphate hydrolases
Length = 168
Score = 81.8 bits (199), Expect = 1e-17
Identities = 27/170 (15%), Positives = 59/170 (33%), Gaps = 13/170 (7%)
Query: 32 YRMVVLGASRVGKSSIVSRFLNGRF---EDQYTPTIEDFHRKVYNIRGDMYQLDILDTSG 88
+ + GA GK S+ +R + ++ P + +
Sbjct: 4 VVLRIYGARNCGKKSLANRIHHFATSMAPERVDPDENGNDYTKMTTF--LLNGREVTLEI 61
Query: 89 NHPFPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKEAAELPMV 148
+ ++I+++++DNR+SF ++ ++I +
Sbjct: 62 LLESTLENSPFRQSKTLYIVMYNMDNRQSFIYATQILERITLANLNNPVPL------QLF 115
Query: 149 ICGNKNDHGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTNVDEMFYVL 198
+ GNK D + + T E + + C + EVSA N +E + +
Sbjct: 116 LIGNKCDLKRN-QVISTNEGKSVART-FKCDFLEVSALLGMNTEETWTTI 163
>gi|232142 [190..392] P-loop containing nucleotide triphosphate hydrolases
Length = 203
Score = 79.3 bits (193), Expect = 6e-17
Identities = 14/144 (9%), Positives = 34/144 (22%), Gaps = 4/144 (2%)
Query: 70 KVYNIRGDMYQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQIL 129
+ D + D G I V + + ++
Sbjct: 4 FETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQ 63
Query: 130 EVKSCLKN--KTKEAAELPMVICGNK-NDHGELCRQVPTTEAELLVSGDENCAYFEVSAK 186
E + K+ + + +++ NK + E + ++ S
Sbjct: 64 EALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIELFVLDDR-LFQERPFSFI 122
Query: 187 KNTNVDEMFYVLFSMAKLPHEMSP 210
++ + Y A P
Sbjct: 123 EDYFPEFARYTTPEDATPEPGEDP 146
>gi|729400 [38..325] P-loop containing nucleotide triphosphate hydrolases
Length = 288
Score = 72.6 bits (175), Expect = 7e-15
Identities = 21/219 (9%), Positives = 56/219 (24%), Gaps = 35/219 (15%)
Query: 29 KNSYRMVVLGASRVGKSSIVSRFLNGR------------------FEDQYTPTIEDFHRK 70
+ ++ GK++ R L E + + I
Sbjct: 2 SKVRNIGIIAHIDAGKTTTTERMLYYAGISKHIGDVDTGDTITDFLEQERSRGITIQSAA 61
Query: 71 VYNIRGDMYQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQILE 130
+ + + ++++DT G+ F ++ D +++ K ++ +
Sbjct: 62 ISFPWRNTFAINLIDTPGHIDFTFEVIRALKVIDSCVVILDAVAGVEAQTEKVWKQSKSK 121
Query: 131 VKSCLKNKTKEAAELPMVICGNKNDHGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTN 190
K C NK + + + K+ T+
Sbjct: 122 PKICFINK----------MDRMGASFNHTVNDLINKFMRGTTTK-PVLVNIPYYRKQPTS 170
Query: 191 VDEMFYVL------FSMAKLPHEMSPALHRKISVQYGDA 223
D +F + + P + ++ +
Sbjct: 171 NDYVFQGVIDVVNGKRLTWNPENPDEIIVDELDGTSLEQ 209
>gi|2136468 [19..195] Lipocalins
Length = 177
Score = 30.8 bits (69), Expect = 0.025
Identities = 19/124 (15%), Positives = 31/124 (24%), Gaps = 15/124 (12%)
Query: 153 KNDHGELCRQVP---TTEAELLVS---GDENCAYFEVSAKKNTNVDEMFYVLFSMAKLPH 206
N E + A+ V + CA + T F + +
Sbjct: 39 DNIVAEFSVDEYGQMSATAKGRVRLLNNWDVCADMVGTFTD-TEDPAKFKMKYWGVASFL 97
Query: 207 EMSPALHRKISVQYGD-AFHPRPFCMRRVKEMDAYG---MVSPFARRPSVNSDLKYIKAK 262
+ H I Y A R+ +D FAR P+ +
Sbjct: 98 QKGNDDHWIIDTDYDTYAV----QYSCRLLNLDGTCADSYSFVFARDPNGFPPEVQRIVR 153
Query: 263 VLRE 266
+E
Sbjct: 154 RRQE 157
>gi|1515341 [77..406] Acid proteases
Length = 330
Score = 28.4 bits (63), Expect = 0.13
Identities = 7/61 (11%), Positives = 11/61 (17%), Gaps = 8/61 (13%)
Query: 62 PTIEDFH--RKVYNIRGDMYQLDILDTSGNHPFPAMRRLSILTGDVFI----LVFSLDNR 115
T+ +I + T IL G F +
Sbjct: 266 ITLTFGFVSGTTVSITERDLVISFFGTCRL-QIIPTTDRIIL-GLPLYRGRCTYFDPIMQ 323
Query: 116 E 116
Sbjct: 324 R 324
>gi|2496715 [53..359] NAD(P)-binding Rossmann-fold domains
Length = 307
Score = 25.4 bits (54), Expect = 1.2
Identities = 12/88 (13%), Positives = 27/88 (30%), Gaps = 2/88 (2%)
Query: 144 ELPMVICGNKNDHGELCRQVPTTEAELLVSGDENCAYFEVSAKKNTNVDEMFYVLFSM-- 201
L + + G N+ + A + S + A + + A + + E+ V+
Sbjct: 179 GLDIPVLGGGNNKYQFVHSDDLASACIAASNVKGFATYNIGAAEFGTMRELLQVVIKHAE 238
Query: 202 AKLPHEMSPALHRKISVQYGDAFHPRPF 229
+ P ++ A P
Sbjct: 239 TGSRIKSIPMGPTALAANLASALGLSPL 266
>gi|2144317 [224..416] Lactate & malate dehydrogenases, C-terminal domain
Length = 193
Score = 24.7 bits (54), Expect = 1.7
Identities = 14/98 (14%), Positives = 29/98 (29%), Gaps = 9/98 (9%)
Query: 46 SIVSRFLN--GRFEDQYTPTIEDFHRKVYNIRGDMYQLDILDTSGNHPFPAMRRLSILTG 103
VS L + Y ++D N +L + A+ G
Sbjct: 99 QFVSLLLGNIEQIHGTYYVPLKDA----NNFPIAPGADQLLPLVDGADYFAIPLTITTKG 154
Query: 104 DVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKE 141
++ ++ + + L + ++K KN K
Sbjct: 155 VSYVDYDIVNRMNDMERNQMLPICVSQLK---KNIDKG 189
>gi|417211 [2..187] P-loop containing nucleotide triphosphate hydrolases
Length = 186
Score = 24.8 bits (54), Expect = 1.8
Identities = 12/48 (25%), Positives = 21/48 (43%), Gaps = 10/48 (20%)
Query: 35 VVLGASRVGKSSIVSRFLNGRFEDQY---------TPTIEDFHRKVYN 73
V+ G S GKS+++ + + D + TP + + K YN
Sbjct: 5 VISGPSGTGKSTLLKKLFA-EYPDSFGFSVSSTTRTPRAGEVNGKDYN 51
>gi|2652959 [169..353] P-loop containing nucleotide triphosphate hydrolases
Length = 185
Score = 23.4 bits (49), Expect = 4.2
Identities = 10/109 (9%), Positives = 27/109 (24%), Gaps = 6/109 (5%)
Query: 34 MVVLGASRVGKSSIVSRFLNGRFEDQYTPTIEDFHRKVYNIRGDMYQLDILDTSGNHPFP 93
++ +G GK+ + D I + G N +
Sbjct: 4 ILFMGGPGGGKTRHA-ARVADSLADNGLVHICM-PDIIRTALGKYKDKYPEWKEANEHYI 61
Query: 94 AMRRLSILTGDVFILVFSLDNR----ESFDEVKRLQKQILEVKSCLKNK 138
+ + + + R +Q+ + + +K+
Sbjct: 62 RGELIPNQLALTLLKAEMGRHPDAMGFFLEGYPREARQVEDFERQVKSV 110
>gi|1175188 [41..508] Periplasmic binding protein-like II
Length = 468
Score = 23.4 bits (50), Expect = 4.4
Identities = 14/120 (11%), Positives = 33/120 (26%), Gaps = 10/120 (8%)
Query: 7 LLQPRAMMKTLSSGNCTLSVPAKNSYRMVVLGA----SRVGKSSIVSRFLNGRFEDQYTP 62
L + + ++ + L AK++ + GA + I++ N + +
Sbjct: 142 LDKASPYLPSMLAHVSLLPHYAKSTEIFISNGAYQLQRQAENQHILTT--NPYYWAKEKV 199
Query: 63 TIEDFHRKVYNIRGDMYQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRESFDEVK 122
+ + Y L D N + F+L + +
Sbjct: 200 IFQ---QVKYQKISVDADLSDFDVVMNPKKVNQNIQDYPQLCTYFYEFNL-SDPVLQKSA 255
>gi|1723793 [7..233] NAD(P)-binding Rossmann-fold domains
Length = 227
Score = 23.1 bits (48), Expect = 5.7
Identities = 10/137 (7%), Positives = 30/137 (21%), Gaps = 10/137 (7%)
Query: 121 VKRLQKQILEVKSCLKNKTKEAAELPM----------VICGNKNDHGELCRQVPTTEAEL 170
+++ + + + +L G+ ++ + ++A +
Sbjct: 15 IQQFFDINPKPDIHIFDVRDLPEKLSKQFTFNVDDIKFHKGDLTSPDDMENAINESKANV 74
Query: 171 LVSGDENCAYFEVSAKKNTNVDEMFYVLFSMAKLPHEMSPALHRKISVQYGDAFHPRPFC 230
+V NV V+ K + + G H
Sbjct: 75 VVHCASPMHGQNPDIYDIVNVKGTRNVIDMCKKCGVNILVYTSSAGVIFNGQDVHNADET 134
Query: 231 MRRVKEMDAYGMVSPFA 247
+ +
Sbjct: 135 WPIPEVPMDAYNETKAI 151
>gi|345362 [61..347] Protein kinases (PK), catalytic core
Length = 287
Score = 23.2 bits (48), Expect = 5.7
Identities = 6/80 (7%), Positives = 19/80 (23%), Gaps = 4/80 (5%)
Query: 60 YTPTIEDFHRKVYNIRGDMYQLDIL---DTSGNHPFPAMRRLSILTGDVFILVFSLDNRE 116
+ + D++ L +G PF L ++ + +
Sbjct: 192 QNSHNWKTKSAYTSEQCDLWALGCTLYFCATGKFPFEHESNNKSLY-HKAVVALTQNPDA 250
Query: 117 SFDEVKRLQKQILEVKSCLK 136
+ + + +
Sbjct: 251 IAMVLVQKGRDPGSSTDMFE 270
>gi|1174715 [19..356] Thiamin-binding
Length = 338
Score = 23.0 bits (49), Expect = 5.8
Identities = 9/31 (29%), Positives = 13/31 (41%)
Query: 47 IVSRFLNGRFEDQYTPTIEDFHRKVYNIRGD 77
+ SR+ G F+ P F +Y I D
Sbjct: 129 MASRYERGLFDPDAEPGASPFDHYIYVIASD 159
>gi|134579 [59..285] P-loop containing nucleotide triphosphate hydrolases
Length = 227
Score = 22.8 bits (47), Expect = 5.9
Identities = 13/124 (10%), Positives = 31/124 (24%), Gaps = 19/124 (15%)
Query: 24 LSVPAKNSYRMVVLGASRVGKSSIVSRFLNGRFEDQYTPTIEDFHRKVYNIRGDMYQLDI 83
L +++ G GKS+++ + L + M L +
Sbjct: 30 LGGGVVPGSAILIGGNPGAGKSTLLLQTLC-------------------KLAQQMKTLYV 70
Query: 84 LDTSGNHPFPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKEAA 143
L D ++ + + Q +++ + S +
Sbjct: 71 TGEESLQQVAMRAHRLGLPTDNLNMLSETSIEQICLIAEEEQPKLMVIDSIQVMHMADVQ 130
Query: 144 ELPM 147
P
Sbjct: 131 SSPG 134
>gi|120586 [1..210] Class II aldolase
Length = 210
Score = 22.9 bits (49), Expect = 6.3
Identities = 16/67 (23%), Positives = 28/67 (40%), Gaps = 9/67 (13%)
Query: 16 TLSSGNCTLSVPAKNSYRMVVLGA----SRVGKSSIVSRFLNGRFEDQYTPTIE-DFHRK 70
++GN + + M++ ++ +S IV NG+ E+ P+ E FH
Sbjct: 24 QGTAGNVS----VRYQDGMLITPTGIPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMA 79
Query: 71 VYNIRGD 77
Y R D
Sbjct: 80 AYQSRPD 86
>gi|2392193 [1..232] Zn metallo-beta-lactamase
Length = 232
Score = 22.7 bits (48), Expect = 6.7
Identities = 5/62 (8%), Positives = 17/62 (27%), Gaps = 3/62 (4%)
Query: 70 KVYNIRGDMYQLDILDTSGNHPFPAMRRLSILTGDVFILVFSLDNRESFDEVKRLQKQIL 129
+ + +Y L + ++ L+ D + + + L +
Sbjct: 12 SITQLSDKVYTYVSLAEIEGWGMVPSNGMIVINNHQAALL---DTPINDAQTEMLVNWVT 68
Query: 130 EV 131
+
Sbjct: 69 DS 70
>gi|2493523 [117..286] Interleukin-1beta converting enzyme (a cysteine protease)
Length = 170
Score = 22.9 bits (49), Expect = 7.0
Identities = 16/89 (17%), Positives = 31/89 (33%), Gaps = 3/89 (3%)
Query: 117 SFDEVKRLQKQILEVKSCLKNKTKEAAELPMVICGNKNDHGELCRQVPTTEAELLVSGDE 176
S +E K++Q + ++ + L ++IC N+ R + + +
Sbjct: 2 SAEEFKKIQDTQGDKIYDVRKREGRKG-LALIIC-NEKFENLNERHGAKVDLDGMTKLLN 59
Query: 177 NCAYFEVSAKKNTNVDEMFYVLFSMAKLP 205
Y +V N EM V+ A
Sbjct: 60 ELGY-QVHPHTNLTKTEMVKVMKEFAAQE 87
>gi|127802 [1..266] Glucosamine 6-phosphate deaminase
Length = 266
Score = 22.6 bits (48), Expect = 7.1
Identities = 8/49 (16%), Positives = 16/49 (32%)
Query: 23 TLSVPAKNSYRMVVLGASRVGKSSIVSRFLNGRFEDQYTPTIEDFHRKV 71
T+ V V++ K+ + + G +T + H K
Sbjct: 187 TVGVGTLLDAEEVMILVLGSQKALALQAAVEGCVNHMWTISCLQLHPKA 235
>gi|417569 [3..336] Aspartate/ornithine carbamoyltransferase, Catalytic-chain
Length = 334
Score = 22.5 bits (48), Expect = 7.4
Identities = 9/52 (17%), Positives = 17/52 (32%), Gaps = 1/52 (1%)
Query: 55 RFEDQYTPTIEDFHRKVYNIRGDMYQLDILDTSGNHPFPAMRRLSILTGDVF 106
R +Q ++ R R + + +T HP P R + +
Sbjct: 256 RMGEQVLKKQDELRRS-ITFRKEFIEKLPENTRFYHPLPRHRVHPTIPTFLD 306
>gi|1346784 [8..238] N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Length = 231
Score = 22.5 bits (48), Expect = 8.2
Identities = 9/45 (20%), Positives = 16/45 (35%), Gaps = 4/45 (8%)
Query: 7 LLQPRAMMKTLSSGNCTLSVPAKNSYRMVVLGA-SRVGKSSIVSR 50
L Q + + G+ + V ++ VVLG + R
Sbjct: 9 LFQVEYAQEAVKKGSTAVGVRGRDI---VVLGVEKKSVAKLQDER 50
>gi|280504 [161..330] Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain
Length = 170
Score = 22.5 bits (48), Expect = 9.2
Identities = 7/31 (22%), Positives = 17/31 (54%)
Query: 97 RLSILTGDVFILVFSLDNRESFDEVKRLQKQ 127
R+ +TG + L +++ + + +EV K+
Sbjct: 83 RVPTITGSLVDLTVNVNAKVTKEEVNAALKK 113
>gi|1515148 [263..496] Protein kinases (PK), catalytic core
Length = 234
Score = 22.3 bits (46), Expect = 9.2
Identities = 6/56 (10%), Positives = 17/56 (29%), Gaps = 3/56 (5%)
Query: 49 SRFLNGRFEDQYTPTIEDFHRKVYNIRGDMYQLDIL---DTSGNHPFPAMRRLSIL 101
+ + R ++ +++ L +L +G +PF +
Sbjct: 140 FTRRSVCVPFMAPEMLTAESRTHHSYSTELWGLGVLLYILLTGKYPFHENSMPLLF 195
>gi|2130121 [317..446] Cyclin-like
Length = 130
Score = 22.5 bits (48), Expect = 9.3
Identities = 12/69 (17%), Positives = 26/69 (37%), Gaps = 3/69 (4%)
Query: 32 YRMVVLGASRVGKSSIV-SRFLNGRFEDQYTPTIEDF-HRKVYNIRGDMYQL-DILDTSG 88
Y ++ S + S+I +RF+ + + T+ + K + + L +
Sbjct: 40 YSLLSYPPSLIAASAIFLARFILQPTKYPWNSTLAHYTQYKPSKLSECVKALHRLCSVGS 99
Query: 89 NHPFPAMRR 97
PA+R
Sbjct: 100 GSNLPAIRE 108
>gi|1673470 [12..233] Protein kinases (PK), catalytic core
Length = 222
Score = 22.4 bits (46), Expect = 9.6
Identities = 9/115 (7%), Positives = 23/115 (19%), Gaps = 1/115 (0%)
Query: 47 IVSRFLNGRFEDQYTPTIEDFHRKVYNIRGDMYQLDILDTSGNHPFPAMRRLSILTGDVF 106
+ + R + T + + A+
Sbjct: 33 VKFVHGSQREKSFKTELSVFRKLDSLGNSKRNGFPQFCENFQVDGYYAIVLSDEGDNLND 92
Query: 107 ILVFSLDNRESFDEVKRLQKQILEVKSCLKNKTKEAAEL-PMVICGNKNDHGELC 160
I + + + V + + S L + A+L +
Sbjct: 93 IRKRNKNLVFNRKNVATVGCAVSRSLSTLHSIGFFHADLHEQNLMVPYPCRKINI 147
>gi|1322391 [16..428] Acid proteases
Length = 413
Score = 22.2 bits (47), Expect = 9.9
Identities = 15/73 (20%), Positives = 18/73 (24%), Gaps = 14/73 (19%)
Query: 62 PTIE-DFHRKVYNIRGDMYQLDILDTS------GNHPFPAMRRLSILTGDVFI----LVF 110
T K Y I L+ S IL G FI +
Sbjct: 343 VTFGMTIGGKQYKIERKNLVLEEDKDSCMIAMTPLSSVG-FGPQWIL-GAPFIRQYCNIH 400
Query: 111 SLDN-RESFDEVK 122
+ N F E K
Sbjct: 401 DMRNNTIGFAEPK 413
Underlying Matrix: BLOSUM62
Number of sequences tested against query: 1187
Number of sequences better than 10.0: 33
Number of calls to ALIGN: 33
Length of query: 278
Total length of test sequences: 256703
Effective length of test sequences: 207231.0
Effective search space size: 49097454.9
Initial X dropoff for ALIGN: 25.0 bits