analysis of sequence from tem22
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
>Tem22_gi|3327232|dbj|BAA31684.1| KIAA0709 protein [Homo sapiens]
MGPGRPAPAPWPRHLLRCVLLLGCLHLGRPGAPGDAALPEPNVFLIFSHGLQGCLEAQGGQVRVTPACNT
SLPAQRWKWVSRNRLFNLGTMQCLGTGWPGTNTTASLGMYECDREALNLRWHCRTLGDQLSLLLGARTSN
ISKPGTLERGDQTRSGQWRIYGSEEDLCALPYHEVYTIQGNSHGKPCTIPFKYDNQWFHGCTSTGREDGH
LWCATTQDYGKDERWGFCPIKSNDCETFWDKDQLTDSCYQFNFQSTLSWREAWASCEQQGADLLSITEIH
EQTYINGLLTGYSSTLWIGLNDLDTSGGWQWSDNSPLKYLNWESDQPDNPSEENCGVIRTESSGGWQNRD
CSIALPYVCKKKPNATAEPTPPDRWANVKVECEPSWQPFQGHCYRLQAEKRSWQESKKACLRGGGDLVSI
HSMAELEFITKQIKQEVEELWIGLNDLKLQMNFEWSDGSLVSFTHWHPFEPNNFRDSLEDCVTIWGPEGR
WNDSPCNQSLPSICKKAGQLSQGAAEEDHGCRKGWTWHSPSCYWLGEDQVTYSEARRLCTDHGSQLVTIT
NRFEQAFVSSLIYNWEGEYFWTALQDLNSTGSFFWLSGDEVMYTHWNRDQPGYSRGGCVALATGSAMGLW
EVKNCTSFRARYICRQSLGTPVTPELPGPDPTPSLTGSCPQGWASDTKLRYCYKVFSSERLQDKKSWVQA
QGACQELGAQLLSLASYEEEHFVANMLNKIFGESEPEIHEQHWFWIGLNRRDPRGGQSWRWSDGVGFSYH
NFDRSRHDDDDIRGCAVLDLASLQWVAMQCDTQLDWICKIPRGTDVREPDDSPQGRREWLRFQEAEYKFF
EHHSTWAQAQRICTWFQAELTSVHSQAELDFLSHNLQKFSRAQEQHWWIGLHTSESDGRFRWTDGSIINF
ISWAPGKPRPVGKDKKCVYMTASREDWGDQRCLTALPYICKRSNVTKETQPPDLPTTALGGCPSDWIQFL
NKCFQVQGQEPQSRVKWSEAQFSCEQQEAQLVTITNPLEQAFITASLPNVTFDLWIGLHASQRDFQWVEQ
EPLMYANWAPGEPSGPSPAPSGNKPTSCAVVLHSPSAHFTGRWDDRSCTEETHGFICQKGTDPSLSPSPA
ALPPAPGTELSYLNGTFRLLQKPLRWHDALLLCESHNASLAYVPDPYTQAFLTQAARGLRTPLWIGLAGE
EGSRRYSWVSEEPLNYVGWQDGEPQQPGGCTYVDVDGAWRTTSCDTKLQGAVCGVSSGPPPPRRISYHGS
CPQGLADSAWIPFREHCYSFHMELLLGHKEARQRCQRAGGAVLSILDEMENVFVWEHLQSYEGQSRGAWL
GMNFNPKGGTLVWQDNTAVNYSNWGPPGLGPSMLSHNSCYWIQSNSGLWRPGACTNITMGVVCKLPRAEQ
SSFSPSALPENPAALVVVLMAVLLLLALLTAALILYRRRQSIERGAFEGARYSRSSSSPTEATEKNILVS
DMEMNEQQE
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
sec.str. with predator
> Tem22_gi|3327232|dbj|BAA31684.1|
. . . . .
1 MGPGRPAPAPWPRHLLRCVLLLGCLHLGRPGAPGDAALPEPNVFLIFSHG 50
____________HHHHHHHHHHHHH_________________EEEEEE__
. . . . .
51 LQGCLEAQGGQVRVTPACNTSLPAQRWKWVSRNRLFNLGTMQCLGTGWPG 100
___________EEEE______________HHHHHH____EEEE_______
. . . . .
101 TNTTASLGMYECDREALNLRWHCRTLGDQLSLLLGARTSNISKPGTLERG 150
_______EEEHHHHHHHHHHHHH____HHHHHHHH_______________
. . . . .
151 DQTRSGQWRIYGSEEDLCALPYHEVYTIQGNSHGKPCTIPFKYDNQWFHG 200
_______EEE______EEEEE_EEEEEE_________EEEE_________
. . . . .
201 CTSTGREDGHLWCATTQDYGKDERWGFCPIKSNDCETFWDKDQLTDSCYQ 250
__________EEEEE___________________________________
. . . . .
251 FNFQSTLSWREAWASCEQQGADLLSITEIHEQTYINGLLTGYSSTLWIGL 300
___HHHHHHHHHHHHHH_____________EEE_EEE________EEE__
. . . . .
301 NDLDTSGGWQWSDNSPLKYLNWESDQPDNPSEENCGVIRTESSGGWQNRD 350
___________________________________EEEEEE_________
. . . . .
351 CSIALPYVCKKKPNATAEPTPPDRWANVKVECEPSWQPFQGHCYRLQAEK 400
___EEE_EEE_______________EEEEEE___________HHHHHHHH
. . . . .
401 RSWQESKKACLRGGGDLVSIHSMAELEFITKQIKQEVEELWIGLNDLKLQ 450
HHHHHHHHHHH_____EEEEHHHHHHHHHHHHHHHHHHHHHH___HHHHH
. . . . .
451 MNFEWSDGSLVSFTHWHPFEPNNFRDSLEDCVTIWGPEGRWNDSPCNQSL 500
HHH_______EEEE_________________EEEE_______________
. . . . .
501 PSICKKAGQLSQGAAEEDHGCRKGWTWHSPSCYWLGEDQVTYSEARRLCT 550
__HHHHHH________________________EEE_____HHHHHHHHHH
. . . . .
551 DHGSQLVTITNRFEQAFVSSLIYNWEGEYFWTALQDLNSTGSFFWLSGDE 600
___EEEEEEHHHHHHHHHHHHHHH___EEEEEE_________EEE_____
. . . . .
601 VMYTHWNRDQPGYSRGGCVALATGSAMGLWEVKNCTSFRARYICRQSLGT 650
EEEEEE___________EEEEE_______EEEEE____EEEEE_______
. . . . .
651 PVTPELPGPDPTPSLTGSCPQGWASDTKLRYCYKVFSSERLQDKKSWVQA 700
____________________________EEEE______HHHHHHHHHHHH
. . . . .
701 QGACQELGAQLLSLASYEEEHFVANMLNKIFGESEPEIHEQHWFWIGLNR 750
___HHHHHHHHHHHHHHHHHHHHHHHHHHHH____HHHHHHHHHHHH___
. . . . .
751 RDPRGGQSWRWSDGVGFSYHNFDRSRHDDDDIRGCAVLDLASLQWVAMQC 800
_______EEEE______________________EEEHHHHHHHHHHHHH_
. . . . .
801 DTQLDWICKIPRGTDVREPDDSPQGRREWLRFQEAEYKFFEHHSTWAQAQ 850
__HHHHHH_________________HHHHHHHHHHHHHHH_____HHHHH
. . . . .
851 RICTWFQAELTSVHSQAELDFLSHNLQKFSRAQEQHWWIGLHTSESDGRF 900
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE_______
. . . . .
901 RWTDGSIINFISWAPGKPRPVGKDKKCVYMTASREDWGDQRCLTALPYIC 950
______EEEEE_____________EEEEEEE_________EEEEEE_EEE
. . . . .
951 KRSNVTKETQPPDLPTTALGGCPSDWIQFLNKCFQVQGQEPQSRVKWSEA 1000
_________________________HHHHHHHHHEEE_____HHHHHHHH
. . . . .
1001 QFSCEQQEAQLVTITNPLEQAFITASLPNVTFDLWIGLHASQRDFQWVEQ 1050
HHHHHHHHHHHH_____HHHHHHHH____EEEE___________EEEE__
. . . . .
1051 EPLMYANWAPGEPSGPSPAPSGNKPTSCAVVLHSPSAHFTGRWDDRSCTE 1100
_EEEEEE___________________EEEEEEE_________________
. . . . .
1101 ETHGFICQKGTDPSLSPSPAALPPAPGTELSYLNGTFRLLQKPLRWHDAL 1150
_____EEE____________________________EEE______HHHHH
. . . . .
1151 LLCESHNASLAYVPDPYTQAFLTQAARGLRTPLWIGLAGEEGSRRYSWVS 1200
HHHH____EEEE______HHHHHHHHHHH____EEEE_______EEEEEE
. . . . .
1201 EEPLNYVGWQDGEPQQPGGCTYVDVDGAWRTTSCDTKLQGAVCGVSSGPP 1250
____EEEEE__________EEEEE____EEEEE_____EEEEEEE_____
. . . . .
1251 PPRRISYHGSCPQGLADSAWIPFREHCYSFHMELLLGHKEARQRCQRAGG 1300
____EEE_________________HHHHHHHHHHHHHHHHHHHHHHH___
. . . . .
1301 AVLSILDEMENVFVWEHLQSYEGQSRGAWLGMNFNPKGGTLVWQDNTAVN 1350
HHHHHHHHHHHHEEEHHHHHH__________________EEEEE___EEE
. . . . .
1351 YSNWGPPGLGPSMLSHNSCYWIQSNSGLWRPGACTNITMGVVCKLPRAEQ 1400
EE_______________EEEEEE___________EEE_____________
. . . . .
1401 SSFSPSALPENPAALVVVLMAVLLLLALLTAALILYRRRQSIERGAFEGA 1450
_____________HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH___
. .
1451 RYSRSSSSPTEATEKNILVSDMEMNEQQE 1479
EEEE______HHHHHH__HHHHHHHH___
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
method : 1
alpha-contents : 26.7 %
beta-contents : 5.3 %
coil-contents : 68.0 %
class : alpha
method : 2
alpha-contents : 15.9 %
beta-contents : 14.9 %
coil-contents : 69.2 %
class : mixed
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
GPI: learning from metazoa
4.98 0.00 0.00 0.00 0.00 0.00 -12.00 0.00 0.00 -3.20 -0.74 -12.00 -12.00 0.00 0.00 0.00 -34.96
6.22 0.00 0.00 0.00 0.00 0.00 -16.00 0.00 0.00 -3.20 -0.74 -12.00 0.00 0.00 0.00 0.00 -25.72
ID: Tem22_gi|3327232|dbj|BAA31684.1| AC: xxx Len: 1470 1:I 1457 Sc: -25.72 Pv: 5.889423e-02 NO_GPI_SITE
GPI: learning from protozoa
0.97 0.00 0.00 0.00 0.00 0.00 -12.00 0.00 0.00 -2.91 -3.74 -12.00 -12.00 0.00 0.00 0.00 -41.68
2.13 -0.57 -0.83 -1.49 0.00 0.00 -8.00 0.00 0.00 -2.91 -3.74 -12.00 -12.00 0.00 0.00 0.00 -39.41
ID: Tem22_gi|3327232|dbj|BAA31684.1| AC: xxx Len: 1470 1:I 1455 Sc: -39.41 Pv: 8.414013e-02 NO_GPI_SITE
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
# SignalP euk predictions
# name Cmax pos ? Ymax pos ? Smax pos ? Smean ?
Tem22_gi|33 0.769 1433 Y 0.695 1433 Y 0.989 1423 Y 0.110 N
# SignalP gram- predictions
# name Cmax pos ? Ymax pos ? Smax pos ? Smean ?
Tem22_gi|33 0.663 850 Y 0.366 1433 Y 0.997 1425 Y 0.127 N
# SignalP gram+ predictions
# name Cmax pos ? Ymax pos ? Smax pos ? Smean ?
Tem22_gi|33 0.612 850 Y 0.473 35 Y 0.995 1429 Y 0.589 Y
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
low complexity regions: SEG 12 2.2 2.5
>Tem22_gi|3327232|dbj|BAA31684.1| KIAA0709 protein [Homo sapiens]
1-1 M
gpgrpapapwprhllrcvlllgclhlg 2-28
29-1058 RPGAPGDAALPEPNVFLIFSHGLQGCLEAQ
GGQVRVTPACNTSLPAQRWKWVSRNRLFNL
GTMQCLGTGWPGTNTTASLGMYECDREALN
LRWHCRTLGDQLSLLLGARTSNISKPGTLE
RGDQTRSGQWRIYGSEEDLCALPYHEVYTI
QGNSHGKPCTIPFKYDNQWFHGCTSTGRED
GHLWCATTQDYGKDERWGFCPIKSNDCETF
WDKDQLTDSCYQFNFQSTLSWREAWASCEQ
QGADLLSITEIHEQTYINGLLTGYSSTLWI
GLNDLDTSGGWQWSDNSPLKYLNWESDQPD
NPSEENCGVIRTESSGGWQNRDCSIALPYV
CKKKPNATAEPTPPDRWANVKVECEPSWQP
FQGHCYRLQAEKRSWQESKKACLRGGGDLV
SIHSMAELEFITKQIKQEVEELWIGLNDLK
LQMNFEWSDGSLVSFTHWHPFEPNNFRDSL
EDCVTIWGPEGRWNDSPCNQSLPSICKKAG
QLSQGAAEEDHGCRKGWTWHSPSCYWLGED
QVTYSEARRLCTDHGSQLVTITNRFEQAFV
SSLIYNWEGEYFWTALQDLNSTGSFFWLSG
DEVMYTHWNRDQPGYSRGGCVALATGSAMG
LWEVKNCTSFRARYICRQSLGTPVTPELPG
PDPTPSLTGSCPQGWASDTKLRYCYKVFSS
ERLQDKKSWVQAQGACQELGAQLLSLASYE
EEHFVANMLNKIFGESEPEIHEQHWFWIGL
NRRDPRGGQSWRWSDGVGFSYHNFDRSRHD
DDDIRGCAVLDLASLQWVAMQCDTQLDWIC
KIPRGTDVREPDDSPQGRREWLRFQEAEYK
FFEHHSTWAQAQRICTWFQAELTSVHSQAE
LDFLSHNLQKFSRAQEQHWWIGLHTSESDG
RFRWTDGSIINFISWAPGKPRPVGKDKKCV
YMTASREDWGDQRCLTALPYICKRSNVTKE
TQPPDLPTTALGGCPSDWIQFLNKCFQVQG
QEPQSRVKWSEAQFSCEQQEAQLVTITNPL
EQAFITASLPNVTFDLWIGLHASQRDFQWV
EQEPLMYANW
apgepsgpspapsg 1059-1072
1073-1112 NKPTSCAVVLHSPSAHFTGRWDDRSCTEET
HGFICQKGTD
pslspspaalppap 1113-1126
1127-1412 GTELSYLNGTFRLLQKPLRWHDALLLCESH
NASLAYVPDPYTQAFLTQAARGLRTPLWIG
LAGEEGSRRYSWVSEEPLNYVGWQDGEPQQ
PGGCTYVDVDGAWRTTSCDTKLQGAVCGVS
SGPPPPRRISYHGSCPQGLADSAWIPFREH
CYSFHMELLLGHKEARQRCQRAGGAVLSIL
DEMENVFVWEHLQSYEGQSRGAWLGMNFNP
KGGTLVWQDNTAVNYSNWGPPGLGPSMLSH
NSCYWIQSNSGLWRPGACTNITMGVVCKLP
RAEQSSFSPSALPENP
aalvvvlmavllllalltaalil 1413-1435
1436-1479 YRRRQSIERGAFEGARYSRSSSSPTEATEK
NILVSDMEMNEQQE
low complexity regions: SEG 25 3.0 3.3
>Tem22_gi|3327232|dbj|BAA31684.1| KIAA0709 protein [Homo sapiens]
1-1 M
gpgrpapapwprhllrcvlllgclhlgrpg 2-39
apgdaalp
40-646 EPNVFLIFSHGLQGCLEAQGGQVRVTPACN
TSLPAQRWKWVSRNRLFNLGTMQCLGTGWP
GTNTTASLGMYECDREALNLRWHCRTLGDQ
LSLLLGARTSNISKPGTLERGDQTRSGQWR
IYGSEEDLCALPYHEVYTIQGNSHGKPCTI
PFKYDNQWFHGCTSTGREDGHLWCATTQDY
GKDERWGFCPIKSNDCETFWDKDQLTDSCY
QFNFQSTLSWREAWASCEQQGADLLSITEI
HEQTYINGLLTGYSSTLWIGLNDLDTSGGW
QWSDNSPLKYLNWESDQPDNPSEENCGVIR
TESSGGWQNRDCSIALPYVCKKKPNATAEP
TPPDRWANVKVECEPSWQPFQGHCYRLQAE
KRSWQESKKACLRGGGDLVSIHSMAELEFI
TKQIKQEVEELWIGLNDLKLQMNFEWSDGS
LVSFTHWHPFEPNNFRDSLEDCVTIWGPEG
RWNDSPCNQSLPSICKKAGQLSQGAAEEDH
GCRKGWTWHSPSCYWLGEDQVTYSEARRLC
TDHGSQLVTITNRFEQAFVSSLIYNWEGEY
FWTALQDLNSTGSFFWLSGDEVMYTHWNRD
QPGYSRGGCVALATGSAMGLWEVKNCTSFR
ARYICRQ
slgtpvtpelpgpdptpsltgscp 647-670
671-1394 QGWASDTKLRYCYKVFSSERLQDKKSWVQA
QGACQELGAQLLSLASYEEEHFVANMLNKI
FGESEPEIHEQHWFWIGLNRRDPRGGQSWR
WSDGVGFSYHNFDRSRHDDDDIRGCAVLDL
ASLQWVAMQCDTQLDWICKIPRGTDVREPD
DSPQGRREWLRFQEAEYKFFEHHSTWAQAQ
RICTWFQAELTSVHSQAELDFLSHNLQKFS
RAQEQHWWIGLHTSESDGRFRWTDGSIINF
ISWAPGKPRPVGKDKKCVYMTASREDWGDQ
RCLTALPYICKRSNVTKETQPPDLPTTALG
GCPSDWIQFLNKCFQVQGQEPQSRVKWSEA
QFSCEQQEAQLVTITNPLEQAFITASLPNV
TFDLWIGLHASQRDFQWVEQEPLMYANWAP
GEPSGPSPAPSGNKPTSCAVVLHSPSAHFT
GRWDDRSCTEETHGFICQKGTDPSLSPSPA
ALPPAPGTELSYLNGTFRLLQKPLRWHDAL
LLCESHNASLAYVPDPYTQAFLTQAARGLR
TPLWIGLAGEEGSRRYSWVSEEPLNYVGWQ
DGEPQQPGGCTYVDVDGAWRTTSCDTKLQG
AVCGVSSGPPPPRRISYHGSCPQGLADSAW
IPFREHCYSFHMELLLGHKEARQRCQRAGG
AVLSILDEMENVFVWEHLQSYEGQSRGAWL
GMNFNPKGGTLVWQDNTAVNYSNWGPPGLG
PSMLSHNSCYWIQSNSGLWRPGACTNITMG
VVCK
lpraeqssfspsalpenpaalvvvlmavll 1395-1462
llalltaalilyrrrqsiergafegarysr
ssssptea
1463-1479 TEKNILVSDMEMNEQQE
low complexity regions: SEG 45 3.4 3.75
>Tem22_gi|3327232|dbj|BAA31684.1| KIAA0709 protein [Homo sapiens]
1-1 M
gpgrpapapwprhllrcvlllgclhlgrpg 2-67
apgdaalpepnvflifshglqgcleaqggq
vrvtpa
68-1394 CNTSLPAQRWKWVSRNRLFNLGTMQCLGTG
WPGTNTTASLGMYECDREALNLRWHCRTLG
DQLSLLLGARTSNISKPGTLERGDQTRSGQ
WRIYGSEEDLCALPYHEVYTIQGNSHGKPC
TIPFKYDNQWFHGCTSTGREDGHLWCATTQ
DYGKDERWGFCPIKSNDCETFWDKDQLTDS
CYQFNFQSTLSWREAWASCEQQGADLLSIT
EIHEQTYINGLLTGYSSTLWIGLNDLDTSG
GWQWSDNSPLKYLNWESDQPDNPSEENCGV
IRTESSGGWQNRDCSIALPYVCKKKPNATA
EPTPPDRWANVKVECEPSWQPFQGHCYRLQ
AEKRSWQESKKACLRGGGDLVSIHSMAELE
FITKQIKQEVEELWIGLNDLKLQMNFEWSD
GSLVSFTHWHPFEPNNFRDSLEDCVTIWGP
EGRWNDSPCNQSLPSICKKAGQLSQGAAEE
DHGCRKGWTWHSPSCYWLGEDQVTYSEARR
LCTDHGSQLVTITNRFEQAFVSSLIYNWEG
EYFWTALQDLNSTGSFFWLSGDEVMYTHWN
RDQPGYSRGGCVALATGSAMGLWEVKNCTS
FRARYICRQSLGTPVTPELPGPDPTPSLTG
SCPQGWASDTKLRYCYKVFSSERLQDKKSW
VQAQGACQELGAQLLSLASYEEEHFVANML
NKIFGESEPEIHEQHWFWIGLNRRDPRGGQ
SWRWSDGVGFSYHNFDRSRHDDDDIRGCAV
LDLASLQWVAMQCDTQLDWICKIPRGTDVR
EPDDSPQGRREWLRFQEAEYKFFEHHSTWA
QAQRICTWFQAELTSVHSQAELDFLSHNLQ
KFSRAQEQHWWIGLHTSESDGRFRWTDGSI
INFISWAPGKPRPVGKDKKCVYMTASREDW
GDQRCLTALPYICKRSNVTKETQPPDLPTT
ALGGCPSDWIQFLNKCFQVQGQEPQSRVKW
SEAQFSCEQQEAQLVTITNPLEQAFITASL
PNVTFDLWIGLHASQRDFQWVEQEPLMYAN
WAPGEPSGPSPAPSGNKPTSCAVVLHSPSA
HFTGRWDDRSCTEETHGFICQKGTDPSLSP
SPAALPPAPGTELSYLNGTFRLLQKPLRWH
DALLLCESHNASLAYVPDPYTQAFLTQAAR
GLRTPLWIGLAGEEGSRRYSWVSEEPLNYV
GWQDGEPQQPGGCTYVDVDGAWRTTSCDTK
LQGAVCGVSSGPPPPRRISYHGSCPQGLAD
SAWIPFREHCYSFHMELLLGHKEARQRCQR
AGGAVLSILDEMENVFVWEHLQSYEGQSRG
AWLGMNFNPKGGTLVWQDNTAVNYSNWGPP
GLGPSMLSHNSCYWIQSNSGLWRPGACTNI
TMGVVCK
lpraeqssfspsalpenpaalvvvlmavll 1395-1462
llalltaalilyrrrqsiergafegarysr
ssssptea
1463-1479 TEKNILVSDMEMNEQQE
low complexity regions: XNU
# Score cutoff = 21, Search from offsets 1 to 4
# both members of each repeat flagged
# lambda = 0.347, K = 0.200, H = 0.664
>Tem22_gi|3327232|dbj|BAA31684.1| KIAA0709 protein [Homo sapiens]
MGPGRPAPAPWPRHLLRCVLLLGCLHLGRPGAPGDAALPEPNVFLIFSHGLQGCLEAQGG
QVRVTPACNTSLPAQRWKWVSRNRLFNLGTMQCLGTGWPGTNTTASLGMYECDREALNLR
WHCRTLGDQLSLLLGARTSNISKPGTLERGDQTRSGQWRIYGSEEDLCALPYHEVYTIQG
NSHGKPCTIPFKYDNQWFHGCTSTGREDGHLWCATTQDYGKDERWGFCPIKSNDCETFWD
KDQLTDSCYQFNFQSTLSWREAWASCEQQGADLLSITEIHEQTYINGLLTGYSSTLWIGL
NDLDTSGGWQWSDNSPLKYLNWESDQPDNPSEENCGVIRTESSGGWQNRDCSIALPYVCK
KKPNATAEPTPPDRWANVKVECEPSWQPFQGHCYRLQAEKRSWQESKKACLRGGGDLVSI
HSMAELEFITKQIKQEVEELWIGLNDLKLQMNFEWSDGSLVSFTHWHPFEPNNFRDSLED
CVTIWGPEGRWNDSPCNQSLPSICKKAGQLSQGAAEEDHGCRKGWTWHSPSCYWLGEDQV
TYSEARRLCTDHGSQLVTITNRFEQAFVSSLIYNWEGEYFWTALQDLNSTGSFFWLSGDE
VMYTHWNRDQPGYSRGGCVALATGSAMGLWEVKNCTSFRARYICRQSLGTPVTPELPGPD
PTPSLTGSCPQGWASDTKLRYCYKVFSSERLQDKKSWVQAQGACQELGAQLLSLASYEEE
HFVANMLNKIFGESEPEIHEQHWFWIGLNRRDPRGGQSWRWSDGVGFSYHNFDRSRHDDD
DIRGCAVLDLASLQWVAMQCDTQLDWICKIPRGTDVREPDDSPQGRREWLRFQEAEYKFF
EHHSTWAQAQRICTWFQAELTSVHSQAELDFLSHNLQKFSRAQEQHWWIGLHTSESDGRF
RWTDGSIINFISWAPGKPRPVGKDKKCVYMTASREDWGDQRCLTALPYICKRSNVTKETQ
PPDLPTTALGGCPSDWIQFLNKCFQVQGQEPQSRVKWSEAQFSCEQQEAQLVTITNPLEQ
AFITASLPNVTFDLWIGLHASQRDFQWVEQEPLMYANWAPGEPSGPSPAPSGNKPTSCAV
VLHSPSAHFTGRWDDRSCTEETHGFICQKGTDPSLSPSPAALPPAPGTELSYLNGTFRLL
QKPLRWHDALLLCESHNASLAYVPDPYTQAFLTQAARGLRTPLWIGLAGEEGSRRYSWVS
EEPLNYVGWQDGEPQQPGGCTYVDVDGAWRTTSCDTKLQGAVCGVssgpppprrISYHGS
CPQGLADSAWIPFREHCYSFHMELLLGHKEARQRCQRAGGAVLSILDEMENVFVWEHLQS
YEGQSRGAWLGMNFNPKGGTLVWQDNTAVNYSNWGPPGLGPSMLSHNSCYWIQSNSGLWR
PGACTNITMGVVCKLPRAEQSSFSPSALPENpaalvvvlmavllllalltaalilyrrrq
SIERGAFEGarysrsssspteateKNILVSDMEMNEQQE
1 - 1245 MGPGRPAPAP WPRHLLRCVL LLGCLHLGRP GAPGDAALPE PNVFLIFSHG LQGCLEAQGG
QVRVTPACNT SLPAQRWKWV SRNRLFNLGT MQCLGTGWPG TNTTASLGMY ECDREALNLR
WHCRTLGDQL SLLLGARTSN ISKPGTLERG DQTRSGQWRI YGSEEDLCAL PYHEVYTIQG
NSHGKPCTIP FKYDNQWFHG CTSTGREDGH LWCATTQDYG KDERWGFCPI KSNDCETFWD
KDQLTDSCYQ FNFQSTLSWR EAWASCEQQG ADLLSITEIH EQTYINGLLT GYSSTLWIGL
NDLDTSGGWQ WSDNSPLKYL NWESDQPDNP SEENCGVIRT ESSGGWQNRD CSIALPYVCK
KKPNATAEPT PPDRWANVKV ECEPSWQPFQ GHCYRLQAEK RSWQESKKAC LRGGGDLVSI
HSMAELEFIT KQIKQEVEEL WIGLNDLKLQ MNFEWSDGSL VSFTHWHPFE PNNFRDSLED
CVTIWGPEGR WNDSPCNQSL PSICKKAGQL SQGAAEEDHG CRKGWTWHSP SCYWLGEDQV
TYSEARRLCT DHGSQLVTIT NRFEQAFVSS LIYNWEGEYF WTALQDLNST GSFFWLSGDE
VMYTHWNRDQ PGYSRGGCVA LATGSAMGLW EVKNCTSFRA RYICRQSLGT PVTPELPGPD
PTPSLTGSCP QGWASDTKLR YCYKVFSSER LQDKKSWVQA QGACQELGAQ LLSLASYEEE
HFVANMLNKI FGESEPEIHE QHWFWIGLNR RDPRGGQSWR WSDGVGFSYH NFDRSRHDDD
DIRGCAVLDL ASLQWVAMQC DTQLDWICKI PRGTDVREPD DSPQGRREWL RFQEAEYKFF
EHHSTWAQAQ RICTWFQAEL TSVHSQAELD FLSHNLQKFS RAQEQHWWIG LHTSESDGRF
RWTDGSIINF ISWAPGKPRP VGKDKKCVYM TASREDWGDQ RCLTALPYIC KRSNVTKETQ
PPDLPTTALG GCPSDWIQFL NKCFQVQGQE PQSRVKWSEA QFSCEQQEAQ LVTITNPLEQ
AFITASLPNV TFDLWIGLHA SQRDFQWVEQ EPLMYANWAP GEPSGPSPAP SGNKPTSCAV
VLHSPSAHFT GRWDDRSCTE ETHGFICQKG TDPSLSPSPA ALPPAPGTEL SYLNGTFRLL
QKPLRWHDAL LLCESHNASL AYVPDPYTQA FLTQAARGLR TPLWIGLAGE EGSRRYSWVS
EEPLNYVGWQ DGEPQQPGGC TYVDVDGAWR TTSCDTKLQG AVCGV
1246 - 1254 ssgpp pprr
1255 - 1411 ISYHGS CPQGLADSAW IPFREHCYSF HMELLLGHKE ARQRCQRAGG AVLSILDEME NVFV
WEHLQS YEGQSRGAWL GMNFNPKGGT LVWQDNTAVN YSNWGPPGLG PSMLSHNSCY WIQS
NSGLWR PGACTNITMG VVCKLPRAEQ SSFSPSALPE N
1412 - 1440 paalvvvlm avllllallt aalilyrrrq
1441 - 1449 SIERGAFEG
1450 - 1464 a rysrsssspt eate
1465 - 1479 KNILVS DMEMNEQQE
low complexity regions: DUST
>Tem22_gi|3327232|dbj|BAA31684.1| KIAA0709 protein [Homo sapiens]
MGPGRPAPAPWPRHLLRCVLLLGCLHLGRPGAPGDAALPEPNVFLIFSHGLQGCLEAQGG
QVRVTPACNTSLPAQRWKWVSRNRLFNLGTMQCLGTGWPGTNTTASLGMYECDREALNLR
WHCRTLGDQLSLLLGARTSNISKPGTLERGDQTRSGQWRIYGSEEDLCALPYHEVYTIQG
NSHGKPCTIPFKYDNQWFHGCTSTGREDGHLWCATTQDYGKDERWGFCPIKSNDCETFWD
KDQLTDSCYQFNFQSTLSWREAWASCEQQGADLLSITEIHEQTYINGLLTGYSSTLWIGL
NDLDTSGGWQWSDNSPLKYLNWESDQPDNPSEENCGVIRTESSGGWQNRDCSIALPYVCK
KKPNATAEPTPPDRWANVKVECEPSWQPFQGHCYRLQAEKRSWQESKKACLRGGGDLVSI
HSMAELEFITKQIKQEVEELWIGLNDLKLQMNFEWSDGSLVSFTHWHPFEPNNFRDSLED
CVTIWGPEGRWNDSPCNQSLPSICKKAGQLSQGAAEEDHGCRKGWTWHSPSCYWLGEDQV
TYSEARRLCTDHGSQLVTITNRFEQAFVSSLIYNWEGEYFWTALQDLNSTGSFFWLSGDE
VMYTHWNRDQPGYSRGGCVALATGSAMGLWEVKNCTSFRARYICRQSLGTPVTPELPGPD
PTPSLTGSCPQGWASDTKLRYCYKVFSSERLQDKKSWVQAQGACQELGAQLLSLASYEEE
HFVANMLNKIFGESEPEIHEQHWFWIGLNRRDPRGGQSWRWSDGVGFSYHNFDRSRHDDD
DIRGCAVLDLASLQWVAMQCDTQLDWICKIPRGTDVREPDDSPQGRREWLRFQEAEYKFF
EHHSTWAQAQRICTWFQAELTSVHSQAELDFLSHNLQKFSRAQEQHWWIGLHTSESDGRF
RWTDGSIINFISWAPGKPRPVGKDKKCVYMTASREDWGDQRCLTALPYICKRSNVTKETQ
PPDLPTTALGGCPSDWIQFLNKCFQVQGQEPQSRVKWSEAQFSCEQQEAQLVTITNPLEQ
AFITASLPNVTFDLWIGLHASQRDFQWVEQEPLMYANWAPGEPSGPSPAPSGNKPTSCAV
VLHSPSAHFTGRWDDRSCTEETHGFICQKGTDPSLSPSPAALPPAPGTELSYLNGTFRLL
QKPLRWHDALLLCESHNASLAYVPDPYTQAFLTQAARGLRTPLWIGLAGEEGSRRYSWVS
EEPLNYVGWQDGEPQQPGGCTYVDVDGAWRTTSCDTKLQGAVCGVSSGPPPPRRISYHGS
CPQGLADSAWIPFREHCYSFHMELLLGHKEARQRCQRAGGAVLSILDEMENVFVWEHLQS
YEGQSRGAWLGMNFNPKGGTLVWQDNTAVNYSNWGPPGLGPSMLSHNSCYWIQSNSGLWR
PGACTNITMGVVCKLPRAEQSSFSPSALPENPAALVVVLMAVLLLLALLTAALILYRRRQ
SIERGAFEGARYSRSSSSPTEATEKNILVSDMEMNEQQE
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
coiled coil prediction for Tem22_gi|3327232|dbj|BAA31684.1|
sequence: 1470 amino acids, 0 residue(s) in coiled coil state
. | . | . | . | . | . 60
MGPGRPAPAP WPRHLLRCVL LLGCLHLGRP GAPGDAALPE PNVFLIFSHG LQGCLEAQGG
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 120
QVRVTPACNT SLPAQRWKWV SRNRLFNLGT MQCLGTGWPG TNTTASLGMY ECDREALNLR
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 180
WHCRTLGDQL SLLLGARTSN ISKPGTLERG DQTRSGQWRI YGSEEDLCAL PYHEVYTIQG
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 240
NSHGKPCTIP FKYDNQWFHG CTSTGREDGH LWCATTQDYG KDERWGFCPI KSNDCETFWD
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 300
KDQLTDSCYQ FNFQSTLSWR EAWASCEQQG ADLLSITEIH EQTYINGLLT GYSSTLWIGL
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 360
NDLDTSGGWQ WSDNSPLKYL NWESDQPDNP SEENCGVIRT ESSGGWQNRD CSIALPYVCK
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 420
KKPNATAEPT PPDRWANVKV ECEPSWQPFQ GHCYRLQAEK RSWQESKKAC LRGGGDLVSI
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~1 * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~555555 555555554~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 480
HSMAELEFIT KQIKQEVEEL WIGLNDLKLQ MNFEWSDGSL VSFTHWHPFE PNNFRDSLED
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ 5555555555 5555555555 5511~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
1111111111 1111111111 1111111111 11~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~122222222 2222222222 ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 540
CVTIWGPEGR WNDSPCNQSL PSICKKAGQL SQGAAEEDHG CRKGWTWHSP SCYWLGEDQV
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 600
TYSEARRLCT DHGSQLVTIT NRFEQAFVSS LIYNWEGEYF WTALQDLNST GSFFWLSGDE
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 660
VMYTHWNRDQ PGYSRGGCVA LATGSAMGLW EVKNCTSFRA RYICRQSLGT PVTPELPGPD
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 720
PTPSLTGSCP QGWASDTKLR YCYKVFSSER LQDKKSWVQA QGACQELGAQ LLSLASYEEE
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 780
HFVANMLNKI FGESEPEIHE QHWFWIGLNR RDPRGGQSWR WSDGVGFSYH NFDRSRHDDD
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 840
DIRGCAVLDL ASLQWVAMQC DTQLDWICKI PRGTDVREPD DSPQGRREWL RFQEAEYKFF
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 900
EHHSTWAQAQ RICTWFQAEL TSVHSQAELD FLSHNLQKFS RAQEQHWWIG LHTSESDGRF
~~~~~~~~~~ ~~~~~~~~~~ ~~~~244444 4444444444 444444~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ----defgab cdefgabcde fgabcd---- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~11111 1111111111 111111~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~1355555 5555555552 2~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 960
RWTDGSIINF ISWAPGKPRP VGKDKKCVYM TASREDWGDQ RCLTALPYIC KRSNVTKETQ
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 1020
PPDLPTTALG GCPSDWIQFL NKCFQVQGQE PQSRVKWSEA QFSCEQQEAQ LVTITNPLEQ
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 1080
AFITASLPNV TFDLWIGLHA SQRDFQWVEQ EPLMYANWAP GEPSGPSPAP SGNKPTSCAV
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 1140
VLHSPSAHFT GRWDDRSCTE ETHGFICQKG TDPSLSPSPA ALPPAPGTEL SYLNGTFRLL
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 1200
QKPLRWHDAL LLCESHNASL AYVPDPYTQA FLTQAARGLR TPLWIGLAGE EGSRRYSWVS
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 1260
EEPLNYVGWQ DGEPQQPGGC TYVDVDGAWR TTSCDTKLQG AVCGVSSGPP PPRRISYHGS
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 1320
CPQGLADSAW IPFREHCYSF HMELLLGHKE ARQRCQRAGG AVLSILDEME NVFVWEHLQS
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 1380
YEGQSRGAWL GMNFNPKGGT LVWQDNTAVN YSNWGPPGLG PSMLSHNSCY WIQSNSGLWR
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . | . | . | . 1440
PGACTNITMG VVCKLPRAEQ SSFSPSALPE NPAALVVVLM AVLLLLALLT AALILYRRRQ
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 -w border
---------- ---------- ---------- ---------- ---------- ---------- * 21 M'95 -w register
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 M'95 +w polar
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 21 MTK -w class
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 28 M'95 -w signif.
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~ * 14 M'95 -w local
. | . | . |
SIERGAFEGA RYSRSSSSPT EATEKNILVS
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~
---------- ---------- ----------
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~
~~~~~~~~~~ ~~~~~~~~~~ ~~~~~~~~~~
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
prediction of transmembrane regions with toppred2
***********************************
*TOPPREDM with eukaryotic function*
***********************************
tem22.___inter___ is a single sequence
Using hydrophobicity file: /bio_software/2D/toppredm/lib/Engelman-Steitz.scale
Using cyt/ext file: /bio_software/2D/toppredm/lib/Cyt-Ext.prok
Using sequence file: tem22.___inter___
(1 sequences)
MGPGRPAPAPWPRHLLRCVLLLGCLHLGRPGAPGDAALPEPNVFLIFSHG
LQGCLEAQGGQVRVTPACNTSLPAQRWKWVSRNRLFNLGTMQCLGTGWPG
TNTTASLGMYECDREALNLRWHCRTLGDQLSLLLGARTSNISKPGTLERG
DQTRSGQWRIYGSEEDLCALPYHEVYTIQGNSHGKPCTIPFKYDNQWFHG
CTSTGREDGHLWCATTQDYGKDERWGFCPIKSNDCETFWDKDQLTDSCYQ
FNFQSTLSWREAWASCEQQGADLLSITEIHEQTYINGLLTGYSSTLWIGL
NDLDTSGGWQWSDNSPLKYLNWESDQPDNPSEENCGVIRTESSGGWQNRD
CSIALPYVCKKKPNATAEPTPPDRWANVKVECEPSWQPFQGHCYRLQAEK
RSWQESKKACLRGGGDLVSIHSMAELEFITKQIKQEVEELWIGLNDLKLQ
MNFEWSDGSLVSFTHWHPFEPNNFRDSLEDCVTIWGPEGRWNDSPCNQSL
PSICKKAGQLSQGAAEEDHGCRKGWTWHSPSCYWLGEDQVTYSEARRLCT
DHGSQLVTITNRFEQAFVSSLIYNWEGEYFWTALQDLNSTGSFFWLSGDE
VMYTHWNRDQPGYSRGGCVALATGSAMGLWEVKNCTSFRARYICRQSLGT
PVTPELPGPDPTPSLTGSCPQGWASDTKLRYCYKVFSSERLQDKKSWVQA
QGACQELGAQLLSLASYEEEHFVANMLNKIFGESEPEIHEQHWFWIGLNR
RDPRGGQSWRWSDGVGFSYHNFDRSRHDDDDIRGCAVLDLASLQWVAMQC
DTQLDWICKIPRGTDVREPDDSPQGRREWLRFQEAEYKFFEHHSTWAQAQ
RICTWFQAELTSVHSQAELDFLSHNLQKFSRAQEQHWWIGLHTSESDGRF
RWTDGSIINFISWAPGKPRPVGKDKKCVYMTASREDWGDQRCLTALPYIC
KRSNVTKETQPPDLPTTALGGCPSDWIQFLNKCFQVQGQEPQSRVKWSEA
QFSCEQQEAQLVTITNPLEQAFITASLPNVTFDLWIGLHASQRDFQWVEQ
EPLMYANWAPGEPSGPSPAPSGNKPTSCAVVLHSPSAHFTGRWDDRSCTE
ETHGFICQKGTDPSLSPSPAALPPAPGTELSYLNGTFRLLQKPLRWHDAL
LLCESHNASLAYVPDPYTQAFLTQAARGLRTPLWIGLAGEEGSRRYSWVS
EEPLNYVGWQDGEPQQPGGCTYVDVDGAWRTTSCDTKLQGAVCGVSSGPP
PPRRISYHGSCPQGLADSAWIPFREHCYSFHMELLLGHKEARQRCQRAGG
AVLSILDEMENVFVWEHLQSYEGQSRGAWLGMNFNPKGGTLVWQDNTAVN
YSNWGPPGLGPSMLSHNSCYWIQSNSGLWRPGACTNITMGVVCKLPRAEQ
SSFSPSALPENPAALVVVLMAVLLLLALLTAALILYRRRQSIERGAFEGA
RYSRSSSSPTEATEKNILVSDMEMNEQQE
(p)rokaryotic or (e)ukaryotic: e
Charge-pair energy: 0
Length of full window (odd number!): 21
Length of core window (odd number!): 11
Number of residues to add to each end of helix: 1
Critical length: 60
Upper cutoff for candidates: 1
Lower cutoff for candidates: 0.6
Total of 16 structures are to be tested
Candidate membrane-spanning segments:
Helix Begin End Score Certainity
1 90 110 0.828 Putative
2 282 302 0.950 Putative
3 612 632 0.866 Putative
4 1020 1040 0.730 Putative
5 1413 1433 2.477 Certain
----------------------------------------------------------------------
Structure 1
Transmembrane segments included in this structure:
Segment 1 2 3 4 5
Loop length 89 171 309 387 372 46
K+R profile + + +
+ + 7.00
CYT-EXT prof 0.17 0.18 0.72
-0.84 -0.20 -
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 0.11
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: 2.11
-> Orientation: N-out
----------------------------------------------------------------------
Structure 2
Transmembrane segments included in this structure:
Segment 2 3 4 5
Loop length 281 309 387 372 46
K+R profile + + 7.00
+ +
CYT-EXT prof -0.54 -0.20 -
0.18 0.72
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.19
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): -1.00
(NEG-POS)/(NEG+POS): 0.0741
NEG: 29.0000
POS: 25.0000
-> Orientation: N-out
CYT-EXT difference: -1.64
-> Orientation: N-in
----------------------------------------------------------------------
Structure 3
Transmembrane segments included in this structure:
Segment 1 3 4 5
Loop length 89 501 387 372 46
K+R profile + + 7.00
+ +
CYT-EXT prof 0.17 -0.20 -
-0.16 0.72
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.12
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: -0.59
-> Orientation: N-in
----------------------------------------------------------------------
Structure 4
Transmembrane segments included in this structure:
Segment 3 4 5
Loop length 611 387 372 46
K+R profile + +
+ 7.00
CYT-EXT prof -0.12 0.72
-0.20 -
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 0.22
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): -5.00
(NEG-POS)/(NEG+POS): 0.1654
NEG: 74.0000
POS: 53.0000
-> Orientation: N-out
CYT-EXT difference: 0.81
-> Orientation: N-out
----------------------------------------------------------------------
Structure 5
Transmembrane segments included in this structure:
Segment 1 2 4 5
Loop length 89 171 717 372 46
K+R profile + + 7.00
+ +
CYT-EXT prof 0.17 0.01 -
-0.84 0.72
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.16
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: 0.30
-> Orientation: N-out
----------------------------------------------------------------------
Structure 6
Transmembrane segments included in this structure:
Segment 2 4 5
Loop length 281 717 372 46
K+R profile + +
+ 7.00
CYT-EXT prof -0.54 0.72
0.01 -
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 0.28
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): -1.00
(NEG-POS)/(NEG+POS): 0.0741
NEG: 29.0000
POS: 25.0000
-> Orientation: N-out
CYT-EXT difference: 0.18
-> Orientation: N-out
----------------------------------------------------------------------
Structure 7
Transmembrane segments included in this structure:
Segment 1 4 5
Loop length 89 909 372 46
K+R profile + +
+ 7.00
CYT-EXT prof 0.17 0.72
-0.16 -
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 0.19
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: 1.05
-> Orientation: N-out
----------------------------------------------------------------------
Structure 8
Transmembrane segments included in this structure:
Segment 4 5
Loop length 1019 372 46
K+R profile + 7.00
+
CYT-EXT prof -0.13 -
0.72
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.33
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): -1.00
(NEG-POS)/(NEG+POS): 0.1312
NEG: 125.0000
POS: 96.0000
-> Orientation: N-out
CYT-EXT difference: -0.85
-> Orientation: N-in
----------------------------------------------------------------------
Structure 9
Transmembrane segments included in this structure:
Segment 1 2 3 5
Loop length 89 171 309 780 46
K+R profile + + 7.00
+ +
CYT-EXT prof 0.17 0.18 -
-0.84 0.27
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.33
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: 0.92
-> Orientation: N-out
----------------------------------------------------------------------
Structure 10
Transmembrane segments included in this structure:
Segment 2 3 5
Loop length 281 309 780 46
K+R profile + +
+ 7.00
CYT-EXT prof -0.54 0.27
0.18 -
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 0.58
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): -1.00
(NEG-POS)/(NEG+POS): 0.0741
NEG: 29.0000
POS: 25.0000
-> Orientation: N-out
CYT-EXT difference: -0.45
-> Orientation: N-in
----------------------------------------------------------------------
Structure 11
Transmembrane segments included in this structure:
Segment 1 3 5
Loop length 89 501 780 46
K+R profile + +
+ 7.00
CYT-EXT prof 0.17 0.27
-0.16 -
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 0.38
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: 0.60
-> Orientation: N-out
----------------------------------------------------------------------
Structure 12
Transmembrane segments included in this structure:
Segment 3 5
Loop length 611 780 46
K+R profile + 7.00
+
CYT-EXT prof -0.12 -
0.27
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.66
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): -5.00
(NEG-POS)/(NEG+POS): 0.1654
NEG: 74.0000
POS: 53.0000
-> Orientation: N-out
CYT-EXT difference: -0.38
-> Orientation: N-in
----------------------------------------------------------------------
Structure 13
Transmembrane segments included in this structure:
Segment 1 2 5
Loop length 89 171 1110 46
K+R profile + +
+ 7.00
CYT-EXT prof 0.17 0.26
-0.84 -
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 0.50
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: 1.27
-> Orientation: N-out
----------------------------------------------------------------------
Structure 14
Transmembrane segments included in this structure:
Segment 2 5
Loop length 281 1110 46
K+R profile + 7.00
+
CYT-EXT prof -0.54 -
0.26
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.87
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): -1.00
(NEG-POS)/(NEG+POS): 0.0741
NEG: 29.0000
POS: 25.0000
-> Orientation: N-out
CYT-EXT difference: -0.80
-> Orientation: N-in
----------------------------------------------------------------------
Structure 15
Transmembrane segments included in this structure:
Segment 1 5
Loop length 89 1302 46
K+R profile + 7.00
+
CYT-EXT prof 0.17 -
0.10
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.57
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: 0.07
-> Orientation: N-out
----------------------------------------------------------------------
Structure 16
Transmembrane segments included in this structure:
Segment 5
Loop length 1412 46
K+R profile +
7.00
CYT-EXT prof 0.11
-
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 1.00
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): -4.00
(NEG-POS)/(NEG+POS): 0.1348
NEG: 160.0000
POS: 122.0000
-> Orientation: N-out
CYT-EXT difference: 0.11
-> Orientation: N-out
----------------------------------------------------------------------
"tem22" 1479
90 110 #f 0.828125
282 302 #f 0.95
612 632 #f 0.865625
1020 1040 #f 0.730208
1413 1433 #t 2.47708
************************************
*TOPPREDM with prokaryotic function*
************************************
tem22.___inter___ is a single sequence
Using hydrophobicity file: /bio_software/2D/toppredm/lib/Engelman-Steitz.scale
Using cyt/ext file: /bio_software/2D/toppredm/lib/Cyt-Ext.prok
Using sequence file: tem22.___inter___
(1 sequences)
MGPGRPAPAPWPRHLLRCVLLLGCLHLGRPGAPGDAALPEPNVFLIFSHG
LQGCLEAQGGQVRVTPACNTSLPAQRWKWVSRNRLFNLGTMQCLGTGWPG
TNTTASLGMYECDREALNLRWHCRTLGDQLSLLLGARTSNISKPGTLERG
DQTRSGQWRIYGSEEDLCALPYHEVYTIQGNSHGKPCTIPFKYDNQWFHG
CTSTGREDGHLWCATTQDYGKDERWGFCPIKSNDCETFWDKDQLTDSCYQ
FNFQSTLSWREAWASCEQQGADLLSITEIHEQTYINGLLTGYSSTLWIGL
NDLDTSGGWQWSDNSPLKYLNWESDQPDNPSEENCGVIRTESSGGWQNRD
CSIALPYVCKKKPNATAEPTPPDRWANVKVECEPSWQPFQGHCYRLQAEK
RSWQESKKACLRGGGDLVSIHSMAELEFITKQIKQEVEELWIGLNDLKLQ
MNFEWSDGSLVSFTHWHPFEPNNFRDSLEDCVTIWGPEGRWNDSPCNQSL
PSICKKAGQLSQGAAEEDHGCRKGWTWHSPSCYWLGEDQVTYSEARRLCT
DHGSQLVTITNRFEQAFVSSLIYNWEGEYFWTALQDLNSTGSFFWLSGDE
VMYTHWNRDQPGYSRGGCVALATGSAMGLWEVKNCTSFRARYICRQSLGT
PVTPELPGPDPTPSLTGSCPQGWASDTKLRYCYKVFSSERLQDKKSWVQA
QGACQELGAQLLSLASYEEEHFVANMLNKIFGESEPEIHEQHWFWIGLNR
RDPRGGQSWRWSDGVGFSYHNFDRSRHDDDDIRGCAVLDLASLQWVAMQC
DTQLDWICKIPRGTDVREPDDSPQGRREWLRFQEAEYKFFEHHSTWAQAQ
RICTWFQAELTSVHSQAELDFLSHNLQKFSRAQEQHWWIGLHTSESDGRF
RWTDGSIINFISWAPGKPRPVGKDKKCVYMTASREDWGDQRCLTALPYIC
KRSNVTKETQPPDLPTTALGGCPSDWIQFLNKCFQVQGQEPQSRVKWSEA
QFSCEQQEAQLVTITNPLEQAFITASLPNVTFDLWIGLHASQRDFQWVEQ
EPLMYANWAPGEPSGPSPAPSGNKPTSCAVVLHSPSAHFTGRWDDRSCTE
ETHGFICQKGTDPSLSPSPAALPPAPGTELSYLNGTFRLLQKPLRWHDAL
LLCESHNASLAYVPDPYTQAFLTQAARGLRTPLWIGLAGEEGSRRYSWVS
EEPLNYVGWQDGEPQQPGGCTYVDVDGAWRTTSCDTKLQGAVCGVSSGPP
PPRRISYHGSCPQGLADSAWIPFREHCYSFHMELLLGHKEARQRCQRAGG
AVLSILDEMENVFVWEHLQSYEGQSRGAWLGMNFNPKGGTLVWQDNTAVN
YSNWGPPGLGPSMLSHNSCYWIQSNSGLWRPGACTNITMGVVCKLPRAEQ
SSFSPSALPENPAALVVVLMAVLLLLALLTAALILYRRRQSIERGAFEGA
RYSRSSSSPTEATEKNILVSDMEMNEQQE
(p)rokaryotic or (e)ukaryotic: p
Charge-pair energy: 0
Length of full window (odd number!): 21
Length of core window (odd number!): 11
Number of residues to add to each end of helix: 1
Critical length: 60
Upper cutoff for candidates: 1
Lower cutoff for candidates: 0.6
Total of 16 structures are to be tested
Candidate membrane-spanning segments:
Helix Begin End Score Certainity
1 90 110 0.828 Putative
2 282 302 0.950 Putative
3 612 632 0.866 Putative
4 1020 1040 0.730 Putative
5 1413 1433 2.477 Certain
----------------------------------------------------------------------
Structure 1
Transmembrane segments included in this structure:
Segment 1 2 3 4 5
Loop length 89 171 309 387 372 46
K+R profile + + +
+ + 7.00
CYT-EXT prof 0.17 0.18 0.72
-0.84 -0.20 -
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 0.11
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: 2.11
-> Orientation: N-out
----------------------------------------------------------------------
Structure 2
Transmembrane segments included in this structure:
Segment 2 3 4 5
Loop length 281 309 387 372 46
K+R profile + + 7.00
+ +
CYT-EXT prof -0.54 -0.20 -
0.18 0.72
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.19
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): -1.00
(NEG-POS)/(NEG+POS): 0.0741
NEG: 29.0000
POS: 25.0000
-> Orientation: N-out
CYT-EXT difference: -1.64
-> Orientation: N-in
----------------------------------------------------------------------
Structure 3
Transmembrane segments included in this structure:
Segment 1 3 4 5
Loop length 89 501 387 372 46
K+R profile + + 7.00
+ +
CYT-EXT prof 0.17 -0.20 -
-0.16 0.72
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.12
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: -0.59
-> Orientation: N-in
----------------------------------------------------------------------
Structure 4
Transmembrane segments included in this structure:
Segment 3 4 5
Loop length 611 387 372 46
K+R profile + +
+ 7.00
CYT-EXT prof -0.12 0.72
-0.20 -
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 0.22
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): -5.00
(NEG-POS)/(NEG+POS): 0.1654
NEG: 74.0000
POS: 53.0000
-> Orientation: N-out
CYT-EXT difference: 0.81
-> Orientation: N-out
----------------------------------------------------------------------
Structure 5
Transmembrane segments included in this structure:
Segment 1 2 4 5
Loop length 89 171 717 372 46
K+R profile + + 7.00
+ +
CYT-EXT prof 0.17 0.01 -
-0.84 0.72
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.16
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: 0.30
-> Orientation: N-out
----------------------------------------------------------------------
Structure 6
Transmembrane segments included in this structure:
Segment 2 4 5
Loop length 281 717 372 46
K+R profile + +
+ 7.00
CYT-EXT prof -0.54 0.72
0.01 -
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 0.28
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): -1.00
(NEG-POS)/(NEG+POS): 0.0741
NEG: 29.0000
POS: 25.0000
-> Orientation: N-out
CYT-EXT difference: 0.18
-> Orientation: N-out
----------------------------------------------------------------------
Structure 7
Transmembrane segments included in this structure:
Segment 1 4 5
Loop length 89 909 372 46
K+R profile + +
+ 7.00
CYT-EXT prof 0.17 0.72
-0.16 -
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 0.19
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: 1.05
-> Orientation: N-out
----------------------------------------------------------------------
Structure 8
Transmembrane segments included in this structure:
Segment 4 5
Loop length 1019 372 46
K+R profile + 7.00
+
CYT-EXT prof -0.13 -
0.72
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.33
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): -1.00
(NEG-POS)/(NEG+POS): 0.1312
NEG: 125.0000
POS: 96.0000
-> Orientation: N-out
CYT-EXT difference: -0.85
-> Orientation: N-in
----------------------------------------------------------------------
Structure 9
Transmembrane segments included in this structure:
Segment 1 2 3 5
Loop length 89 171 309 780 46
K+R profile + + 7.00
+ +
CYT-EXT prof 0.17 0.18 -
-0.84 0.27
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.33
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: 0.92
-> Orientation: N-out
----------------------------------------------------------------------
Structure 10
Transmembrane segments included in this structure:
Segment 2 3 5
Loop length 281 309 780 46
K+R profile + +
+ 7.00
CYT-EXT prof -0.54 0.27
0.18 -
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 0.58
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): -1.00
(NEG-POS)/(NEG+POS): 0.0741
NEG: 29.0000
POS: 25.0000
-> Orientation: N-out
CYT-EXT difference: -0.45
-> Orientation: N-in
----------------------------------------------------------------------
Structure 11
Transmembrane segments included in this structure:
Segment 1 3 5
Loop length 89 501 780 46
K+R profile + +
+ 7.00
CYT-EXT prof 0.17 0.27
-0.16 -
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 0.38
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: 0.60
-> Orientation: N-out
----------------------------------------------------------------------
Structure 12
Transmembrane segments included in this structure:
Segment 3 5
Loop length 611 780 46
K+R profile + 7.00
+
CYT-EXT prof -0.12 -
0.27
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.66
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): -5.00
(NEG-POS)/(NEG+POS): 0.1654
NEG: 74.0000
POS: 53.0000
-> Orientation: N-out
CYT-EXT difference: -0.38
-> Orientation: N-in
----------------------------------------------------------------------
Structure 13
Transmembrane segments included in this structure:
Segment 1 2 5
Loop length 89 171 1110 46
K+R profile + +
+ 7.00
CYT-EXT prof 0.17 0.26
-0.84 -
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 0.50
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: 1.27
-> Orientation: N-out
----------------------------------------------------------------------
Structure 14
Transmembrane segments included in this structure:
Segment 2 5
Loop length 281 1110 46
K+R profile + 7.00
+
CYT-EXT prof -0.54 -
0.26
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.87
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): -1.00
(NEG-POS)/(NEG+POS): 0.0741
NEG: 29.0000
POS: 25.0000
-> Orientation: N-out
CYT-EXT difference: -0.80
-> Orientation: N-in
----------------------------------------------------------------------
Structure 15
Transmembrane segments included in this structure:
Segment 1 5
Loop length 89 1302 46
K+R profile + 7.00
+
CYT-EXT prof 0.17 -
0.10
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: 7.00
Tm probability: 0.57
-> Orientation: N-in
Charge-difference over N-terminal Tm (+-15 residues): 4.00
(NEG-POS)/(NEG+POS): -0.5000
NEG: 3.0000
POS: 9.0000
-> Orientation: N-in
CYT-EXT difference: 0.07
-> Orientation: N-out
----------------------------------------------------------------------
Structure 16
Transmembrane segments included in this structure:
Segment 5
Loop length 1412 46
K+R profile +
7.00
CYT-EXT prof 0.11
-
For CYT-EXT profile neg. values indicate cytoplasmic preference.
K+R difference: -7.00
Tm probability: 1.00
-> Orientation: N-out
Charge-difference over N-terminal Tm (+-15 residues): -4.00
(NEG-POS)/(NEG+POS): 0.1348
NEG: 160.0000
POS: 122.0000
-> Orientation: N-out
CYT-EXT difference: 0.11
-> Orientation: N-out
----------------------------------------------------------------------
"tem22" 1479
90 110 #f 0.828125
282 302 #f 0.95
612 632 #f 0.865625
1020 1040 #f 0.730208
1413 1433 #t 2.47708
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
NOW EXECUTING: /bio_software/1D/stat/saps/saps-stroh/SAPS.SSPA/saps /people/maria/tem22.___saps___
SAPS. Version of April 11, 1996.
Date run: Tue Oct 31 14:55:04 2000
File: /people/maria/tem22.___saps___
ID Tem22_gi|3327232|dbj|BAA31684.1|
DE KIAA0709 protein [Homo sapiens]
number of residues: 1479; molecular weight: 166.7 kdal
1 MGPGRPAPAP WPRHLLRCVL LLGCLHLGRP GAPGDAALPE PNVFLIFSHG LQGCLEAQGG
61 QVRVTPACNT SLPAQRWKWV SRNRLFNLGT MQCLGTGWPG TNTTASLGMY ECDREALNLR
121 WHCRTLGDQL SLLLGARTSN ISKPGTLERG DQTRSGQWRI YGSEEDLCAL PYHEVYTIQG
181 NSHGKPCTIP FKYDNQWFHG CTSTGREDGH LWCATTQDYG KDERWGFCPI KSNDCETFWD
241 KDQLTDSCYQ FNFQSTLSWR EAWASCEQQG ADLLSITEIH EQTYINGLLT GYSSTLWIGL
301 NDLDTSGGWQ WSDNSPLKYL NWESDQPDNP SEENCGVIRT ESSGGWQNRD CSIALPYVCK
361 KKPNATAEPT PPDRWANVKV ECEPSWQPFQ GHCYRLQAEK RSWQESKKAC LRGGGDLVSI
421 HSMAELEFIT KQIKQEVEEL WIGLNDLKLQ MNFEWSDGSL VSFTHWHPFE PNNFRDSLED
481 CVTIWGPEGR WNDSPCNQSL PSICKKAGQL SQGAAEEDHG CRKGWTWHSP SCYWLGEDQV
541 TYSEARRLCT DHGSQLVTIT NRFEQAFVSS LIYNWEGEYF WTALQDLNST GSFFWLSGDE
601 VMYTHWNRDQ PGYSRGGCVA LATGSAMGLW EVKNCTSFRA RYICRQSLGT PVTPELPGPD
661 PTPSLTGSCP QGWASDTKLR YCYKVFSSER LQDKKSWVQA QGACQELGAQ LLSLASYEEE
721 HFVANMLNKI FGESEPEIHE QHWFWIGLNR RDPRGGQSWR WSDGVGFSYH NFDRSRHDDD
781 DIRGCAVLDL ASLQWVAMQC DTQLDWICKI PRGTDVREPD DSPQGRREWL RFQEAEYKFF
841 EHHSTWAQAQ RICTWFQAEL TSVHSQAELD FLSHNLQKFS RAQEQHWWIG LHTSESDGRF
901 RWTDGSIINF ISWAPGKPRP VGKDKKCVYM TASREDWGDQ RCLTALPYIC KRSNVTKETQ
961 PPDLPTTALG GCPSDWIQFL NKCFQVQGQE PQSRVKWSEA QFSCEQQEAQ LVTITNPLEQ
1021 AFITASLPNV TFDLWIGLHA SQRDFQWVEQ EPLMYANWAP GEPSGPSPAP SGNKPTSCAV
1081 VLHSPSAHFT GRWDDRSCTE ETHGFICQKG TDPSLSPSPA ALPPAPGTEL SYLNGTFRLL
1141 QKPLRWHDAL LLCESHNASL AYVPDPYTQA FLTQAARGLR TPLWIGLAGE EGSRRYSWVS
1201 EEPLNYVGWQ DGEPQQPGGC TYVDVDGAWR TTSCDTKLQG AVCGVSSGPP PPRRISYHGS
1261 CPQGLADSAW IPFREHCYSF HMELLLGHKE ARQRCQRAGG AVLSILDEME NVFVWEHLQS
1321 YEGQSRGAWL GMNFNPKGGT LVWQDNTAVN YSNWGPPGLG PSMLSHNSCY WIQSNSGLWR
1381 PGACTNITMG VVCKLPRAEQ SSFSPSALPE NPAALVVVLM AVLLLLALLT AALILYRRRQ
1441 SIERGAFEGA RYSRSSSSPT EATEKNILVS DMEMNEQQE
--------------------------------------------------------------------------------
COMPOSITIONAL ANALYSIS (extremes relative to: swp23s)
A : 93( 6.3%); C : 56( 3.8%); D : 72( 4.9%); E : 96( 6.5%); F : 51( 3.4%)
G :122( 8.2%); H : 40( 2.7%); I : 47( 3.2%); K : 46( 3.1%); L :135( 9.1%)
M : 19( 1.3%); N : 58( 3.9%); P : 94( 6.4%); Q : 90( 6.1%); R : 83( 5.6%)
S :128( 8.7%); T : 84( 5.7%); V : 58( 3.9%); W++: 67( 4.5%); Y : 40( 2.7%)
KR : 129 ( 8.7%); ED : 168 ( 11.4%); AGP : 309 ( 20.9%);
KRED : 297 ( 20.1%); KR-ED : -39 ( -2.6%); FIKMNY : 261 ( 17.6%);
LVIFM : 310 ( 21.0%); ST : 212 ( 14.3%).
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CHARGE DISTRIBUTIONAL ANALYSIS
1 0000+00000 00+000+000 00000000+0 0000-0000- 0000000000 00000-0000
61 00+0000000 00000+0+00 0+0+000000 0000000000 0000000000 -0-+-0000+
121 000+000-00 000000+000 00+0000-+0 -00+0000+0 000---0000 000-000000
181 0000+00000 0+0-000000 00000+--00 0000000-00 +--+000000 +00-0-000-
241 +-000-0000 000000000+ -00000-000 0-00000-00 -000000000 0000000000
301 0-0-000000 00-0000+00 00-0-00-00 0--00000+0 -0000000+- 000000000+
361 ++00000-00 00-+0000+0 -0-0000000 0000+000-+ +000-0++00 0+000-0000
421 0000-0-000 +00+0-0--0 00000-0+00 000-00-000 000000000- 0000+-00--
481 0000000-0+ 00-0000000 0000++0000 00000---00 0++0000000 000000--00
541 000-0++000 -000000000 0+0-000000 00000-0-00 00000-0000 00000000--
601 0000000+-0 0000+00000 0000000000 -0+00000+0 +000+00000 0000-0000-
661 0000000000 00000-0+0+ 000+0000-+ 00-++00000 00000-0000 0000000---
721 00000000+0 00-0-0-00- 000000000+ +-0+00000+ 00-0000000 00-+0+0---
781 -0+00000-0 0000000000 -000-000+0 0+00-0+-0- -0000++-00 +00-0-0+00
841 -000000000 +0000000-0 0000000-0- 0000000+00 +00-000000 0000-0-0+0
901 +00-000000 000000+0+0 00+-++0000 000+--00-0 +000000000 ++0000+-00
961 00-0000000 0000-00000 0+0000000- 000+0+00-0 0000-00-00 00000000-0
1021 0000000000 00-0000000 00+-0000-0 -000000000 0-00000000 000+000000
1081 0000000000 0+0--+000- -0000000+0 0-00000000 00000000-0 0000000+00
1141 0+00+00-00 000-000000 0000-00000 000000+00+ 000000000- -00++00000
1201 --00000000 -0-0000000 000-0-000+ 0000-0+000 0000000000 00++000000
1261 000000-000 000+-00000 00-00000+- 0+0+00+000 000000--0- 00000-0000
1321 0-000+0000 000000+000 0000-00000 0000000000 0000000000 000000000+
1381 0000000000 000+00+0-0 000000000- 0000000000 0000000000 000000+++0
1441 00-+000-00 +00+000000 -00-+00000 -0-00-00-
A. CHARGE CLUSTERS.
Positive charge clusters (cmin = 9/30 or 12/45 or 15/60): none
Negative charge clusters (cmin = 11/30 or 14/45 or 17/60): none
Mixed charge clusters (cmin = 15/30 or 21/45 or 26/60): none
B. HIGH SCORING (UN)CHARGED SEGMENTS.
There are no high scoring positive charge segments.
There are no high scoring negative charge segments.
There are no high scoring mixed charge segments.
________________________________
High scoring uncharged segments:
score= 1.00 frequency= 0.799 ( LAGSVTIPNFQYHMCW )
score= 0.00 frequency= 0.000 ( BZX )
score= -8.00 frequency= 0.201 ( KEDR )
Expected score/letter: -0.807
M_0.01= 54.86; M_0.05= 45.40
1) From 1327 to 1436: length=110, score=47.00 *
(pocket at 1394 to 1399: length= 6, score=-21.00)
1327 GAWLGMNFNP KGGTLVWQDN TAVNYSNWGP PGLGPSMLSH NSCYWIQSNS
1377 GLWRPGACTN ITMGVVC |KLP RAE| QSSFSPS ALPENPAALV VVLMAVLLLL
1427 ALLTAALILY
L: 17(15.5%); A: 11(10.0%);
C. CHARGE RUNS AND PATTERNS.
pattern (+)| (-)| (*)| (0)| (+0)| (-0)| (*0)|(+00)|(-00)|(*00)| (H.)|(H..)|
lmin0 5 | 5 | 7 | 46 | 10 | 11 | 14 | 12 | 13 | 16 | 8 | 10 |
lmin1 6 | 7 | 9 | 55 | 12 | 13 | 16 | 14 | 16 | 20 | 9 | 12 |
lmin2 7 | 8 | 10 | 62 | 13 | 14 | 18 | 16 | 18 | 22 | 10 | 13 |
(Significance level: 0.010000; Minimal displayed length: 6)
There are no charge runs or patterns exceeding the given minimal lengths.
Run count statistics:
+ runs >= 3: 2, at 360; 1437;
- runs >= 4: 1, at 778;
* runs >= 5: 0
0 runs >= 31: 1, at 1346;
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DISTRIBUTION OF OTHER AMINO ACID TYPES
1. HIGH SCORING SEGMENTS.
__________________________________
High scoring hydrophobic segments:
2.00 (LVIFM) 1.00 (AGYCW) 0.00 (BZX) -2.00 (PH) -4.00 (STNQ)
-8.00 (KEDR)
Expected score/letter: -2.087
M_0.01= 23.85; M_0.05= 20.24
1) From 1413 to 1436: length= 24, score=35.00 **
1413 AALVVVLMAV LLLLALLTAA LILY
L: 10(41.7%); A: 6(25.0%); V: 4(16.7%);
____________________________________
High scoring transmembrane segments:
5.00 (LVIF) 2.00 (AGM) 0.00 (BZX) -1.00 (YCW) -2.00 (ST)
-6.00 (P) -8.00 (H) -10.00 (NQ) -16.00 (KR) -17.00 (ED)
Expected score/letter: -4.022
M_0.01= 52.69; M_0.05= 44.49; M_0.30= 34.74
1) From 1413 to 1435: length= 23, score=87.00 **
1413 AALVVVLMAV LLLLALLTAA LIL
L: 10(43.5%); A: 6(26.1%); V: 4(17.4%);
2. SPACINGS OF C.
H2N-17-C-5-C-29-C-13-C-24-C-18-C-10-C-44-C-18-C-13-C-11-C-14-C-6-C-12-C-17-C-68-C-15-C-7-C-22-C-10-C-16-C-70-C-14-C-7-C-16-C-10-C-16-C-68-C-16-C-8-C-24-C-12-C-21-C-80-C-14-C-7-C-44-C-73-C-14-C-7-C-21-C-10-C-20-C-73-C-19-C-8-C-45-C-66-C-13-C-8-C-17-C-15-C-17-C-73-C-14-C-8-C-86-COOH
2*. SPACINGS OF C and H. (additional deluxe function for ALEX)
H2N-13-H-3-C-5-C-1-H-22-H-4-C-13-C-24-C-18-C-9-H-C-44-C-4-H-9-H-3-C-11-H-1-C-8-H-2-C-14-C-6-C-12-C-17-C-13-H-54-C-15-C-7-C-22-C-9-H-C-16-C-10-H-43-H-1-H-13-C-14-C-7-C-14-H-1-C-6-H-3-C-16-C-2-H-52-H-12-C-16-C-8-C-24-C-12-C-21-C-16-H-17-H-2-H-27-H-6-H-7-C-14-C-7-C-33-H-H-9-C-10-H-9-H-11-H-5-H-34-C-14-C-7-C-21-C-10-C-20-C-34-H-38-C-4-H-4-H-
9-C-4-H-3-C-39-H-5-C-2-H-63-C-13-C-8-C-14-H-2-C-14-H-C-3-H-6-H-6-C-21-H-48-H-2-C-14-C-8-C-86-COOH
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REPETITIVE STRUCTURES.
A. SEPARATED, TANDEM, AND PERIODIC REPEATS: amino acid alphabet.
Repeat core block length: 5
Aligned matching blocks:
[ 261- 269] EAWASCEQQ
[ 999-1007] EAQFSCEQQ
______________________________
[ 278- 282] EIHEQ
[ 737- 741] EIHEQ
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[ 296- 303]-( -7)-[ 297- 301]
[ 440- 447]-( -7)-[ 441- 445]
[ ]--------[ 745- 749]
[ 296- 303] LWIGLNDL
[ 440- 447] LWIGLNDL
[ 297- 301] WIGLN
[ 441- 445] WIGLN
[ 745- 749] WIGLN
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[ 306- 310] SGGWQ
[ 343- 347] SGGWQ
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[ 555- 567] QLVTITNRFEQAF
[1010-1022] QLVTITNPLEQAF
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[ 667- 672] GSCPQG
[1259-1264] GSCPQG
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[ 888- 892] WIGLH
[1035-1039] WIGLH
______________________________
[1034-1038] LWIGL
[1183-1187] LWIGL
B. SEPARATED AND TANDEM REPEATS: 11-letter reduced alphabet.
(i= LVIF; += KR; -= ED; s= AG; o= ST; n= NQ; a= YW; p= P; h= H; m= M; c= C)
Repeat core block length: 9
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MULTIPLETS.
A. AMINO ACID ALPHABET.
1. Total number of amino acid multiplets: 78 (Expected range: 54--108)
2. Histogram of spacings between consecutive amino acid multiplets:
(1-5) 23 (6-10) 8 (11-20) 21 (>=21) 27
3. Clusters of amino acid multiplets (cmin = 10/30 or 13/45 or 15/60): none
B. CHARGE ALPHABET.
1. Total number of charge multiplets: 32 (Expected range: 11-- 43)
14 +plets (f+: 8.7%), 18 -plets (f-: 11.4%)
Total number of charge altplets: 27 (Critical number: 47)
2. Histogram of spacings between consecutive charge multiplets:
(1-5) 4 (6-10) 5 (11-20) 3 (>=21) 21
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PERIODICITY ANALYSIS.
A. AMINO ACID ALPHABET (core: 4; !-core: 5)
Location Period Element Copies Core Errors
6- 13 2 P. 4 4 0
657- 664 2 P. 4 4 0
778- 781 1 D 4 4 0
1130-1169 10 L......... 4 4 0
1249-1252 1 P 4 4 0
1423-1429 1 L 6 4 1
1455-1458 1 S 4 4 0
B. CHARGE ALPHABET ({+= KR; -= ED; 0}; core: 5; !-core: 7)
and HYDROPHOBICITY ALPHABET ({*= KRED; i= LVIF; 0}; core: 6; !-core: 9)
Location Period Element Copies Core Errors
433- 486 9 i.....*.0 6 6 /0/./././././2/./0/
1415-1429 1 i 13 6 2
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SPACING ANALYSIS.
Location (Quartile) Spacing Rank P-value Interpretation
18- 24 (1.) C( 6)C 57 of 57 0.0000 large minimal spacing
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
Start with Pfam (from /data/patterns/pfam)
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file: /data/patterns/pfam/Pfam
Sequence file: tem22
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: Tem22_gi|3327232|dbj|BAA31684.1| KIAA0709 protein [Homo sapiens]
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
lectin_c Lectin C-type domain 557.3 9.9e-164 8
fn2 Fibronectin type II domain 75.1 1.8e-27 1
Ricin_B_lectin Similarity to lectin domain of ricin 31.4 2.1e-05 1
Involucrin Involucrin repeat 6.7 60 2
Gram_pos_anchor Gram positive anchor 4.3 12 1
granulin Granulins 2.6 21 1
Xlink Extracellular link domain 1.9 3 1
oxidored_q4 NADH-ubiquinone/plastoquinone oxidore 1.1 46 1
Fork_head Fork head domain 0.9 89 1
THF_DHG_CYH Tetrahydrofolate dehydrogenase/cycloh 0.1 47 1
biopterin_H Biopterin-dependent aromatic amino ac -1.0 41 1
neur Neuraminidases -1.2 39 1
hemocyanin hemocyanin family -1.4 58 1
IBR IBR domain -28.9 87 1
MIase Muconolactone delta-isomerase -44.7 38 1
DB DB module -49.9 78 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
Involucrin 1/2 55 64 .. 1 10 [] 2.5 7.2e+02
Ricin_B_lectin 1/1 41 161 .. 1 145 [] 31.4 2.1e-05
fn2 1/1 187 228 .. 1 42 [] 75.1 1.8e-27
lectin_c 1/8 264 359 .. 1 111 [] 107.4 2.8e-28
THF_DHG_CYH 1/1 429 440 .. 1 12 [. 0.1 47
lectin_c 2/8 408 504 .. 1 111 [] 128.0 1.7e-34
IBR 1/1 489 532 .. 1 72 [] -28.9 87
Xlink 1/1 539 556 .. 14 31 .. 1.9 3
lectin_c 3/8 547 644 .. 1 111 [] 85.6 1e-21
granulin 1/1 669 684 .. 28 43 .] 2.6 21
hemocyanin 1/1 760 783 .. 784 807 .] -1.4 58
lectin_c 4/8 702 808 .. 1 111 [] 46.3 6.7e-10
biopterin_H 1/1 866 882 .. 320 336 .] -1.0 41
Fork_head 1/1 863 885 .. 78 101 .] 0.9 89
MIase 1/1 815 896 .. 1 94 [] -44.7 38
neur 1/1 902 912 .. 477 487 .] -1.2 39
lectin_c 5/8 851 950 .. 1 111 [] 78.6 1.2e-19
DB 1/1 917 985 .. 1 124 [] -49.9 78
Involucrin 2/2 1004 1013 .. 1 10 [] 5.1 1.6e+02
lectin_c 6/8 1002 1107 .. 1 111 [] 78.7 1.2e-19
lectin_c 7/8 1151 1243 .. 1 111 [] 43.7 4e-09
lectin_c 8/8 1293 1393 .. 1 111 [] 29.0 6.1e-05
Gram_pos_anchor 1/1 1400 1439 .. 1 41 [] 4.3 12
oxidored_q4 1/1 1415 1444 .. 53 82 .] 1.1 46
Alignments of top-scoring domains:
Involucrin: domain 1 of 2, from 55 to 64: score 2.5, E = 7.2e+02
*->leqQegQlkh<-*
le Q gQ+ +
Tem22_gi|3 55 LEAQGGQVRV 64
Ricin_B_lectin: domain 1 of 1, from 41 to 161: score 31.4, E = 2.1e-05
*->rsvfvitilvngnsgrClDvnsssesdGnqvqlwnChsnpgknQkWs
++vf+i + Cl ++++ + +C+ +Q W+
Tem22_gi|3 41 PNVFLIF---SHGLQGCLEAQGGQV-----RVTPACNT-SLPAQRWK 78
ltYdesdgeirsvvnndkCLtv..nanspgseVklyqCdsatsdnQkWel
++ s ++++ ++ +CL+++ +++++ ++ +y+Cd +n++W++
Tem22_gi|3 79 WV---SRNRLFN-LGTMQCLGTgwPGTNTTASLGMYECDR-EALNLRWHC 123
nndglignkIllnlvntglvLdvkgsdtqngTklilytcsggrnqqWlpt
+ g+ ++ l+L +++s+++ +l + r+ qW ++
Tem22_gi|3 124 RTLGDQLS----------LLLGARTSNISKP--GTLERGDQTRSGQWRIY 161
<-*
Tem22_gi|3 - -
fn2: domain 1 of 1, from 187 to 228: score 75.1, E = 1.8e-27
*->CvFPFiynGksYesCTseGRsdgrlWCsTTaNYDrDkkwGFC<-*
C PF+y +++++ CTs+GR dg+lWC+TT++Y +D +wGFC
Tem22_gi|3 187 CTIPFKYDNQWFHGCTSTGREDGHLWCATTQDYGKDERWGFC 228
lectin_c: domain 1 of 8, from 264 to 359: score 107.4, E = 2.8e-28
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn.yaWIGLtdinkgep
+ C+++ g++L+sI Eq +++ l++ + + +WIGL+d +
Tem22_gi|3 264 ASCEQQ--GADLLSITEIHEQTYINGLLTGYSsTLWIGLNDLD---- 304
tegtWvWsdGslpvnytnWakipgePnnwrhkggkedCvelytktpkang
t+g+W+Wsd+s p+ y nW +++P+n + e+C++++t + +g
Tem22_gi|3 305 TSGGWQWSDNS-PLKYLNWE--SDQPDN----PSEENCGVIRTES---SG 344
kWnDepCdsklpyvC<-*
W +++C+ lpyvC
Tem22_gi|3 345 GWQNRDCSIALPYVC 359
THF_DHG_CYH: domain 1 of 1, from 429 to 440: score 0.1, E = 47
*->ireelaeevaal<-*
i +++++ev++l
Tem22_gi|3 429 ITKQIKQEVEEL 440
lectin_c: domain 2 of 8, from 408 to 504: score 128.0, E = 1.7e-34
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn.yaWIGLtdinkgep
+aC r gg+LvsI+s +E +F+++ +k++ + +WIGL+d +
Tem22_gi|3 408 KACLRG--GGDLVSIHSMAELEFITKQIKQEVeELWIGLNDLK---- 448
tegtWvWsdGslpvnytnWakipgePnnwrhkggkedCvelytktpkang
+++++WsdGs v++t+W p ePnn r + edCv +++ p g
Tem22_gi|3 449 LQMNFEWSDGS-LVSFTHWH--PFEPNNFR--DSLEDCVTIWG--P--EG 489
kWnDepCdsklpyvC<-*
+WnD pC+++lp +C
Tem22_gi|3 490 RWNDSPCNQSLPSIC 504
IBR: domain 1 of 1, from 489 to 532: score -28.9, E = 87
*->eKYekfmvrsyveknpdlkwCPgpdCsyavrltevssstelaepprV
++ ++ ++ +++ + C++a +l+ a +
Tem22_gi|3 489 GRWNDSPCNQSLPS---------I-CKKAGQLSQ------GAAEEDH 519
eCkkPaCgtsFCfkCgaeWHapvsC<-*
C+k g +WH+p sC
Tem22_gi|3 520 GCRK-----------GWTWHSP-SC 532
Xlink: domain 1 of 1, from 539 to 556: score 1.9, E = 3
*->kltFeEAqaaClrqgAri<-*
++t+ EA+++C + g+++
Tem22_gi|3 539 QVTYSEARRLCTDHGSQL 556
lectin_c: domain 3 of 8, from 547 to 644: score 85.6, E = 1e-21
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn..yaWIGLtdinkge
C+ + g++Lv+I ++ Eq+F++sl+ + ++y+W+ L d n
Tem22_gi|3 547 RLCTDH--GSQLVTITNRFEQAFVSSLIYNWEgeYFWTALQDLN--- 588
ptegtWvWsdGslpvnytnWakipgePnnwrhkggkedCvelytktpkan
+ g+++W G+ +v yt+W+ +++P ++ Cv+l t+++
Tem22_gi|3 589 -STGSFFWLSGD-EVMYTHWN--RDQPGY-----SRGGCVALATGSA--M 627
gkWnDepCdskl.pyvC<-*
g+W ++C s + +y+C
Tem22_gi|3 628 GLWEVKNCTSFRaRYIC 644
granulin: domain 1 of 1, from 669 to 684: score 2.6, E = 21
*->CPqGfhCdadggrClq<-*
CPqG d++ ++C +
Tem22_gi|3 669 CPQGWASDTKLRYCYK 684
hemocyanin: domain 1 of 1, from 760 to 783: score -1.4, E = 58
*->kdvdtsdkpykkddltyhdsdhlv<-*
++ d + ++y + d+++hd d+
Tem22_gi|3 760 RWSDGVGFSYHNFDRSRHDDDDIR 783
lectin_c: domain 4 of 8, from 702 to 808: score 46.3, E = 6.7e-10
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn...........yaWI
aCq++ g++L+s s+eE F+++++++ ++++++ ++++ +WI
Tem22_gi|3 702 GACQEL--GAQLLSLASYEEEHFVANMLNKIFgesepeiheqhWFWI 746
GLtdinkgeptegtWvWsdGslpvnytnWakipgePnnwrhkggkedCve
GL++++ + +W WsdG +y n +++ + ++ r C++
Tem22_gi|3 747 GLNRRD--PRGGQSWRWSDGV-GFSYHNFDRSRHDDDDIR------GCAV 787
lytktpkangkWnDepCdsklpyvC<-*
l + +W + Cd++l ++C
Tem22_gi|3 788 LDLA----SLQWVAMQCDTQLDWIC 808
biopterin_H: domain 1 of 1, from 866 to 882: score -1.0, E = 41
*->nsElqiLsnAlakisrk<-*
+ El+ Ls+ l+k sr
Tem22_gi|3 866 QAELDFLSHNLQKFSRA 882
Fork_head: domain 1 of 1, from 863 to 885: score 0.9, E = 89
*->ldPeaedmFenkkkkgsflkrrrr<-*
++ +ae +F+ + ++f++++ +
Tem22_gi|3 863 VHSQAELDFLS-HNLQKFSRAQEQ 885
MIase: domain 1 of 1, from 815 to 896: score -44.7, E = 38
*->MLFhVrMtVniPddmpadraerikarEkAySqeLQrqGkWrhLWRPP
+V+ Pdd p r+e ++ +E++y + +W R
Tem22_gi|3 815 -------DVREPDDSPQGRREWLRFQEAEY-KFFEHHSTWAQAQR-- 851
LiaGeYaNiSvFDVeSndeLheiLssLPLFPYMkieVtpLcrHPSsv<-*
i + + V S +eL+ + +L F+ + + H S
Tem22_gi|3 852 -IC-TWFQAELTSVHSQAELDFLSHNLQKFSRAQEQHWWIGLHTSES 896
neur: domain 1 of 1, from 902 to 912: score -1.2, E = 39
*->WpDGAdlpffi<-*
W DG +++f +
Tem22_gi|3 902 WTDGSIINFIS 912
lectin_c: domain 5 of 8, from 851 to 950: score 78.6, E = 1.2e-19
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn.....yaWIGLtdin
C+ ++ L+s++sq+E dFl+ + ++ + +++ WIGL+ +
Tem22_gi|3 851 RICTWF--QAELTSVHSQAELDFLSHNLQKFSraqeqHWWIGLHTSE 895
kgeptegtWvWsdGslpvnytnWakipgePnnwrhkggkedCvelytktp
++g + W+dGs n+ +Wa pg P+ g + +Cv + +++
Tem22_gi|3 896 ----SDGRFRWTDGS-IINFISWA--PGKPRP---VGKDKKCVYMTASRE 935
kangkWnDepCdsklpyvC<-*
W D C + lpy+C
Tem22_gi|3 936 ----DWGDQRCLTALPYIC 950
DB: domain 1 of 1, from 917 to 985: score -49.9, E = 78
*->CCesdrkvpeeClskkCsfntysksalnmvvqkFkqlmffkqddsCp
+ r v ++ kkC++ t s + + ++C
Tem22_gi|3 917 --K-PRPVGKD---KKCVYMTASREDW-------------GD-QRCL 943
lkgkalpemlfCAAqgrDHtdCClrnGVttPqTlaGkkCLtfCdqspgPR
alp + C+r+ Vt+ + +q+p+
Tem22_gi|3 944 T---ALPYI-------------CKRSNVTK-------E-----TQPPD-- 963
RFWfvtqldksYvpCldrfes.IkqCFy<-*
+t ++ C +++ + CF+
Tem22_gi|3 964 ---LPTTALG---GCPSDWIQfLNKCFQ 985
Involucrin: domain 2 of 2, from 1004 to 1013: score 5.1, E = 1.6e+02
*->leqQegQlkh<-*
+eqQe Ql
Tem22_gi|3 1004 CEQQEAQLVT 1013
lectin_c: domain 6 of 8, from 1002 to 1107: score 78.7, E = 1.2e-19
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn.yaWIGLtdinkgep
C+++ ++Lv+I ++ Eq+F++ +++v+ ++WIGL+
Tem22_gi|3 1002 FSCEQQ--EAQLVTITNPLEQAFITASLPNVTfDLWIGLHA------ 1040
tegtWvWsdGslpvnytnWakipgePnnwr.h.kggk.edC.velytktp
++++++W+ + p+ y nWa pgeP++ + + g+k+ C v+l++ ++
Tem22_gi|3 1041 SQRDFQWVEQE-PLMYANWA--PGEPSGPSpApSGNKpTSCaVVLHSPSA 1087
kangkWnDepCdskl.pyvC<-*
+g+W+D++C + ++++C
Tem22_gi|3 1088 HFTGRWDDRSCTEEThGFIC 1107
lectin_c: domain 7 of 8, from 1151 to 1243: score 43.7, E = 4e-09
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn.yaWIGLtdinkgep
l C+++ +++L+ + ++ q+Fl++ + ++ +WIGL +
Tem22_gi|3 1151 LLCESH--NASLAYVPDPYTQAFLTQAARGLRtPLWIGLAGEE---- 1191
tegtWvWsdGslpvnytnWakipgePnnwrhkggkedCvelytktpkang
++ + W+ + p+ny W +geP ++ C + +g
Tem22_gi|3 1192 GSRRYSWVSEE-PLNYVGWQ--DGEP------QQPGGCTYVDV-----DG 1227
kWnDepCdskl.pyvC<-*
W+ +Cd+kl+++vC
Tem22_gi|3 1228 AWRTTSCDTKLqGAVC 1243
lectin_c: domain 8 of 8, from 1293 to 1393: score 29.0, E = 6.1e-05
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn....yaWIGLtdink
+ Cqr+ gg +sI ++ E+ F+++++ + ++++ aW+G++ ++
Tem22_gi|3 1293 QRCQRA--GGAVLSILDEMENVFVWEHLQSYEgqsrGAWLGMNFNP- 1336
geptegtWvWsdGslpvnytnWak.ipgePnnwrhkggkedCvelytktp
++gt vW d++ +vny+nW + g+++ + C +++
Tem22_gi|3 1337 ---KGGTLVWQDNT-AVNYSNWGPpGLGPSML-----SHNSCYWIQSN-- 1375
kangkWnDepCdskl.pyvC<-*
+g+W+ +C ++vC
Tem22_gi|3 1376 --SGLWRPGACTNITmGVVC 1393
Gram_pos_anchor: domain 1 of 1, from 1400 to 1439: score 4.3, E = 12
*->apaskkkkLPkTGeenatnlltaaGllalvagagllavkrK<-*
+ + + +LP+ ++ ++l+a+ ll +++ a+l +++r+
Tem22_gi|3 1400 QSSFSPSALPENP-AALVVVLMAVLLLLALLTAALILYRRR 1439
oxidored_q4: domain 1 of 1, from 1415 to 1444: score 1.1, E = 46
*->smlgflsaliFlfiLtiGLvYEWrqGaLeW<-*
++++++ l++l++Lt+ L+ r+ + e
Tem22_gi|3 1415 LVVVLMAVLLLLALLTAALILYRRRQSIER 1444
//
Start with PfamFrag (from /data/patterns/pfam)
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file: /data/patterns/pfam/PfamFrag
Sequence file: tem22
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: Tem22_gi|3327232|dbj|BAA31684.1| KIAA0709 protein [Homo sapiens]
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
lectin_c Lectin C-type domain 553.9 2.2e-189 8
fn2 Fibronectin type II domain 75.1 1.8e-27 1
Ricin_B_lectin Similarity to lectin domain of ricin b 29.6 6.6e-07 1
MORN MORN motif 3.1 62 1
OPR Octicosapeptide repeat 3.0 45 1
granulin Granulins 2.6 21 1
Xlink Extracellular link domain 1.9 3 1
oxidored_q4 NADH-ubiquinone/plastoquinone oxidored 1.1 46 1
LexA_DNA_bind LexA DNA binding domain 0.9 51 1
Fork_head Fork head domain 0.9 89 1
5-FTHF_cyc-lig 5-formyltetrahydrofolate cyclo-ligase 0.8 36 1
Pico_P2A Picornavirus core protein 2A 0.4 70 1
Nuclease S1/P1 Nuclease 0.4 26 1
Lipase_GDSL Lipase/Acylhydrolase with GDSL-like mo 0.3 56 1
mce mce related protein 0.2 99 1
THF_DHG_CYH Tetrahydrofolate dehydrogenase/cyclohy 0.1 47 1
biopterin_H Biopterin-dependent aromatic amino aci -1.0 41 1
neur Neuraminidases -1.2 39 1
hemocyanin hemocyanin family -1.4 58 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
Ricin_B_lectin 1/1 41 161 .. 1 145 [] 29.6 6.6e-07
fn2 1/1 187 228 .. 1 42 [] 75.1 1.8e-27
Lipase_GDSL 1/1 295 310 .. 132 146 .] 0.3 56
lectin_c 1/8 264 359 .. 1 111 [] 105.5 2.7e-35
OPR 1/1 415 434 .. 11 30 .] 3.0 45
THF_DHG_CYH 1/1 429 440 .. 1 12 [. 0.1 47
lectin_c 2/8 408 504 .. 1 111 [] 126.2 2.1e-42
Xlink 1/1 539 556 .. 14 31 .. 1.9 3
lectin_c 3/8 547 644 .. 1 111 [] 83.7 8.1e-28
granulin 1/1 669 684 .. 28 43 .] 2.6 21
Nuclease 1/1 724 735 .. 304 315 .] 0.4 26
hemocyanin 1/1 760 783 .. 784 807 .] -1.4 58
lectin_c 4/8 702 808 .. 1 111 [] 44.5 2.5e-14
5-FTHF_cyc-lig 1/1 820 854 .. 1 35 [. 0.8 36
biopterin_H 1/1 866 882 .. 320 336 .] -1.0 41
Fork_head 1/1 863 885 .. 78 101 .] 0.9 89
MORN 1/1 898 907 .. 14 23 .] 3.1 62
neur 1/1 902 912 .. 477 487 .] -1.2 39
LexA_DNA_bind 1/1 910 920 .. 55 65 .] 0.9 51
lectin_c 5/8 851 950 .. 1 111 [] 76.8 2e-25
Pico_P2A 1/1 1043 1050 .. 120 127 .] 0.4 70
lectin_c 6/8 1002 1107 .. 1 111 [] 76.8 1.9e-25
lectin_c 7/8 1151 1243 .. 1 111 [] 41.9 1.9e-13
lectin_c 8/8 1293 1356 .. 1 69 [. 36.7 1.2e-11
oxidored_q4 1/1 1415 1444 .. 53 82 .] 1.1 46
mce 1/1 1419 1444 .. 1 28 [. 0.2 99
Alignments of top-scoring domains:
Ricin_B_lectin: domain 1 of 1, from 41 to 161: score 29.6, E = 6.6e-07
*->rsvfvitilvngnsgrClDvnsssesdGnqvqlwnChsnpgknQkWs
++vf+i + Cl ++++ + +C+ +Q W+
Tem22_gi|3 41 PNVFLIF---SHGLQGCLEAQGGQV-----RVTPACNT-SLPAQRWK 78
ltYdesdgeirsvvnndkCLtv..nanspgseVklyqCdsatsdnQkWel
++ s ++++ ++ +CL+++ +++++ ++ +y+Cd +n++W++
Tem22_gi|3 79 WV---SRNRLFN-LGTMQCLGTgwPGTNTTASLGMYECDR-EALNLRWHC 123
nndglignkIllnlvntglvLdvkgsdtqngTklilytcsggrnqqWlpt
+ g+ ++ l+L +++s+++ +l + r+ qW ++
Tem22_gi|3 124 RTLGDQLS----------LLLGARTSNISKP--GTLERGDQTRSGQWRIY 161
<-*
Tem22_gi|3 - -
fn2: domain 1 of 1, from 187 to 228: score 75.1, E = 1.8e-27
*->CvFPFiynGksYesCTseGRsdgrlWCsTTaNYDrDkkwGFC<-*
C PF+y +++++ CTs+GR dg+lWC+TT++Y +D +wGFC
Tem22_gi|3 187 CTIPFKYDNQWFHGCTSTGREDGHLWCATTQDYGKDERWGFC 228
Lipase_GDSL: domain 1 of 1, from 295 to 310: score 0.3, E = 56
*->tielGgNDlc.dyfrd<-*
t+++G NDl+++++++
Tem22_gi|3 295 TLWIGLNDLDtSGGWQ 310
lectin_c: domain 1 of 8, from 264 to 359: score 105.5, E = 2.7e-35
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn.yaWIGLtdinkgep
+ C+++ g++L+sI Eq +++ l++ + + +WIGL+d +
Tem22_gi|3 264 ASCEQQ--GADLLSITEIHEQTYINGLLTGYSsTLWIGLNDLD---- 304
tegtWvWsdGslpvnytnWakipgePnnwrhkggkedCvelytktpkang
t+g+W+Wsd+s p+ y nW +++P+n + e+C++++t + +g
Tem22_gi|3 305 TSGGWQWSDNS-PLKYLNWE--SDQPDN----PSEENCGVIRTES---SG 344
kWnDepCdsklpyvC<-*
W +++C+ lpyvC
Tem22_gi|3 345 GWQNRDCSIALPYVC 359
OPR: domain 1 of 1, from 415 to 434: score 3.0, E = 45
*->GDlvtisSdeeleeAlrlar<-*
GDlv+i S +ele+ ++ +
Tem22_gi|3 415 GDLVSIHSMAELEFITKQIK 434
THF_DHG_CYH: domain 1 of 1, from 429 to 440: score 0.1, E = 47
*->ireelaeevaal<-*
i +++++ev++l
Tem22_gi|3 429 ITKQIKQEVEEL 440
lectin_c: domain 2 of 8, from 408 to 504: score 126.2, E = 2.1e-42
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn.yaWIGLtdinkgep
+aC r gg+LvsI+s +E +F+++ +k++ + +WIGL+d +
Tem22_gi|3 408 KACLRG--GGDLVSIHSMAELEFITKQIKQEVeELWIGLNDLK---- 448
tegtWvWsdGslpvnytnWakipgePnnwrhkggkedCvelytktpkang
+++++WsdGs v++t+W p ePnn r + edCv +++ p g
Tem22_gi|3 449 LQMNFEWSDGS-LVSFTHWH--PFEPNNFR--DSLEDCVTIWG--P--EG 489
kWnDepCdsklpyvC<-*
+WnD pC+++lp +C
Tem22_gi|3 490 RWNDSPCNQSLPSIC 504
Xlink: domain 1 of 1, from 539 to 556: score 1.9, E = 3
*->kltFeEAqaaClrqgAri<-*
++t+ EA+++C + g+++
Tem22_gi|3 539 QVTYSEARRLCTDHGSQL 556
lectin_c: domain 3 of 8, from 547 to 644: score 83.7, E = 8.1e-28
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn..yaWIGLtdinkge
C+ + g++Lv+I ++ Eq+F++sl+ + ++y+W+ L d n
Tem22_gi|3 547 RLCTDH--GSQLVTITNRFEQAFVSSLIYNWEgeYFWTALQDLN--- 588
ptegtWvWsdGslpvnytnWakipgePnnwrhkggkedCvelytktpkan
+ g+++W G+ +v yt+W+ +++P ++ Cv+l t+++
Tem22_gi|3 589 -STGSFFWLSGD-EVMYTHWN--RDQPGY-----SRGGCVALATGSA--M 627
gkWnDepCdskl.pyvC<-*
g+W ++C s + +y+C
Tem22_gi|3 628 GLWEVKNCTSFRaRYIC 644
granulin: domain 1 of 1, from 669 to 684: score 2.6, E = 21
*->CPqGfhCdadggrClq<-*
CPqG d++ ++C +
Tem22_gi|3 669 CPQGWASDTKLRYCYK 684
Nuclease: domain 1 of 1, from 724 to 735: score 0.4, E = 26
*->AaiLNrifsses<-*
A++LN if+ +
Tem22_gi|3 724 ANMLNKIFGESE 735
hemocyanin: domain 1 of 1, from 760 to 783: score -1.4, E = 58
*->kdvdtsdkpykkddltyhdsdhlv<-*
++ d + ++y + d+++hd d+
Tem22_gi|3 760 RWSDGVGFSYHNFDRSRHDDDDIR 783
lectin_c: domain 4 of 8, from 702 to 808: score 44.5, E = 2.5e-14
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn...........yaWI
aCq++ g++L+s s+eE F+++++++ ++++++ ++++ +WI
Tem22_gi|3 702 GACQEL--GAQLLSLASYEEEHFVANMLNKIFgesepeiheqhWFWI 746
GLtdinkgeptegtWvWsdGslpvnytnWakipgePnnwrhkggkedCve
GL++++ + +W WsdG +y n +++ + ++ r C++
Tem22_gi|3 747 GLNRRD--PRGGQSWRWSDGV-GFSYHNFDRSRHDDDDIR------GCAV 787
lytktpkangkWnDepCdsklpyvC<-*
l + +W + Cd++l ++C
Tem22_gi|3 788 LDLA----SLQWVAMQCDTQLDWIC 808
5-FTHF_cyc-lig: domain 1 of 1, from 820 to 854: score 0.8, E = 36
*->rRralsreerlqeseelsqllkslpeqqkakrvaa<-*
++ + r e+l+ +e +++++ ++++ +a+r+++
Tem22_gi|3 820 DDSPQGRREWLRFQEAEYKFFEHHSTWAQAQRICT 854
biopterin_H: domain 1 of 1, from 866 to 882: score -1.0, E = 41
*->nsElqiLsnAlakisrk<-*
+ El+ Ls+ l+k sr
Tem22_gi|3 866 QAELDFLSHNLQKFSRA 882
Fork_head: domain 1 of 1, from 863 to 885: score 0.9, E = 89
*->ldPeaedmFenkkkkgsflkrrrr<-*
++ +ae +F+ + ++f++++ +
Tem22_gi|3 863 VHSQAELDFLS-HNLQKFSRAQEQ 885
MORN: domain 1 of 1, from 898 to 907: score 3.1, E = 62
*->GvytwanGdr<-*
G+++w++G+
Tem22_gi|3 898 GRFRWTDGSI 907
neur: domain 1 of 1, from 902 to 912: score -1.2, E = 39
*->WpDGAdlpffi<-*
W DG +++f +
Tem22_gi|3 902 WTDGSIINFIS 912
LexA_DNA_bind: domain 1 of 1, from 910 to 920: score 0.9, E = 51
*->yIerdpgkpRa<-*
+I + pgkpR
Tem22_gi|3 910 FISWAPGKPRP 920
lectin_c: domain 5 of 8, from 851 to 950: score 76.8, E = 2e-25
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn.....yaWIGLtdin
C+ ++ L+s++sq+E dFl+ + ++ + +++ WIGL+ +
Tem22_gi|3 851 RICTWF--QAELTSVHSQAELDFLSHNLQKFSraqeqHWWIGLHTSE 895
kgeptegtWvWsdGslpvnytnWakipgePnnwrhkggkedCvelytktp
++g + W+dGs n+ +Wa pg P+ g + +Cv + +++
Tem22_gi|3 896 ----SDGRFRWTDGS-IINFISWA--PGKPRP---VGKDKKCVYMTASRE 935
kangkWnDepCdsklpyvC<-*
W D C + lpy+C
Tem22_gi|3 936 ----DWGDQRCLTALPYIC 950
Pico_P2A: domain 1 of 1, from 1043 to 1050: score 0.4, E = 70
*->RDLlwlEe<-*
RD++w+E
Tem22_gi|3 1043 RDFQWVEQ 1050
lectin_c: domain 6 of 8, from 1002 to 1107: score 76.8, E = 1.9e-25
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn.yaWIGLtdinkgep
C+++ ++Lv+I ++ Eq+F++ +++v+ ++WIGL+
Tem22_gi|3 1002 FSCEQQ--EAQLVTITNPLEQAFITASLPNVTfDLWIGLHA------ 1040
tegtWvWsdGslpvnytnWakipgePnnwr.h.kggk.edC.velytktp
++++++W+ + p+ y nWa pgeP++ + + g+k+ C v+l++ ++
Tem22_gi|3 1041 SQRDFQWVEQE-PLMYANWA--PGEPSGPSpApSGNKpTSCaVVLHSPSA 1087
kangkWnDepCdskl.pyvC<-*
+g+W+D++C + ++++C
Tem22_gi|3 1088 HFTGRWDDRSCTEEThGFIC 1107
lectin_c: domain 7 of 8, from 1151 to 1243: score 41.9, E = 1.9e-13
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn.yaWIGLtdinkgep
l C+++ +++L+ + ++ q+Fl++ + ++ +WIGL +
Tem22_gi|3 1151 LLCESH--NASLAYVPDPYTQAFLTQAARGLRtPLWIGLAGEE---- 1191
tegtWvWsdGslpvnytnWakipgePnnwrhkggkedCvelytktpkang
++ + W+ + p+ny W +geP ++ C + +g
Tem22_gi|3 1192 GSRRYSWVSEE-PLNYVGWQ--DGEP------QQPGGCTYVDV-----DG 1227
kWnDepCdskl.pyvC<-*
W+ +Cd+kl+++vC
Tem22_gi|3 1228 AWRTTSCDTKLqGAVC 1243
lectin_c: domain 8 of 8, from 1293 to 1356: score 36.7, E = 1.2e-11
*->laCqrekpgghLvsIqsqeEqdFlqslvkavn....yaWIGLtdink
+ Cqr+ gg +sI ++ E+ F+++++ + ++++ aW+G++ ++
Tem22_gi|3 1293 QRCQRA--GGAVLSILDEMENVFVWEHLQSYEgqsrGAWLGMNFNP- 1336
geptegtWvWsdGslpvnytnWakip<-*
++gt vW d++ +vny+nW p
Tem22_gi|3 1337 ---KGGTLVWQDNT-AVNYSNWG--P 1356
oxidored_q4: domain 1 of 1, from 1415 to 1444: score 1.1, E = 46
*->smlgflsaliFlfiLtiGLvYEWrqGaLeW<-*
++++++ l++l++Lt+ L+ r+ + e
Tem22_gi|3 1415 LVVVLMAVLLLLALLTAALILYRRRQSIER 1444
mce: domain 1 of 1, from 1419 to 1444: score 0.2, E = 99
*->lglfllvalvvllvvllwfglrslPlpr<-*
l ++ll+++++ ++++l+++++s + r
Tem22_gi|3 1419 LMAVLLLLALLTAALILYRRRQS--IER 1444
//
Start with Repeat Library (from /data/patterns/repeats-Miguel-Andrade/hmm)
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file: /data/patterns/repeats-Miguel-Andrade/hmm/repeats.hmm-lib
Sequence file: tem22
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: Tem22_gi|3327232|dbj|BAA31684.1| KIAA0709 protein [Homo sapiens]
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
[no hits above thresholds]
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
[no hits above thresholds]
Alignments of top-scoring domains:
[no hits above thresholds]
//
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
Start with Prosite
---------------------------------------------------------
| ppsearch (c) 1994 EMBL Data Library |
| based on MacPattern (c) 1990-1994 R. Fuchs |
---------------------------------------------------------
PROSITE pattern search started: Tue Oct 31 14:58:49 2000
Sequence file: tem22
----------------------------------------
Sequence Tem22_gi|3327232|dbj|BAA31684.1| (1479 residues):
Matching pattern PS00001 ASN_GLYCOSYLATION:
69: NTSL
102: NTTA
140: NISK
364: NATA
497: NQSL
588: NSTG
634: NCTS
954: NVTK
1029: NVTF
1134: NGTF
1157: NASL
1350: NYSN
1386: NITM
Total matches: 13
Matching pattern PS00004 CAMP_PHOSPHO_SITE:
1194: RRYS
1253: RRIS
1438: RRQS
Total matches: 3
Matching pattern PS00005 PKC_PHOSPHO_SITE:
204: TGR
258: SWR
406: SKK
560: TNR
637: SFR
688: SER
758: SWR
1041: SQR
1090: TGR
1136: TFR
1193: SRR
1463: TEK
Total matches: 12
Matching pattern PS00006 CK2_PHOSPHO_SITE:
125: TLGD
163: SEED
204: TGRE
215: TTQD
237: TFWD
258: SWRE
275: SITE
370: TPPD
402: SWQE
422: SMAE
477: SLED
541: TYSE
597: SGDE
716: SYEE
734: SEPE
775: SRHD
802: TQLD
865: SQAE
894: SESD
933: SRED
1041: SQRD
1097: SCTE
1221: TYVD
1232: TSCD
1304: SILD
1458: SPTE
1470: SDME
Total matches: 27
Matching pattern PS00007 TYR_PHOSPHO_SITE:
1444: RGAFEGARY
Total matches: 1
Matching pattern PS00008 MYRISTYL:
50: GLQGCL
53: GCLEAQ
89: GTMQCL
100: GTNTTA
108: GMYECD
135: GARTSN
180: GNSHGK
200: GCTSTG
287: GLLTGY
336: GVIRTE
344: GGWQNR
458: GSLVSF
520: GCRKGW
616: GGCVAL
624: GSAMGL
764: GVGFSY
890: GLHTSE
905: GSIINF
970: GGCPSD
1037: GLHASQ
1072: GNKPTS
1110: GTDPSL
1127: GTELSY
1227: GAWRTT
1240: GAVCGV
1264: GLADSA
1300: GAVLSI
1323: GQSRGA
1327: GAWLGM
1358: GLGPSM
1382: GACTNI
1445: GAFEGA
1449: GARYSR
Total matches: 33
Matching pattern PS00009 AMIDATION:
824: QGRR
Total matches: 1
Matching pattern PS00016 RGD:
149: RGD
Total matches: 1
Matching pattern PS00023 FIBRONECTIN_2:
187: CTIPFKYDNQWFHGCTSTGREDGHLWCATTQDYGKDERWGFC
Total matches: 1
Matching pattern PS00615 C_TYPE_LECTIN_1:
335: CGVIRTESSGGWQNRDCSIALPYVC
481: CVTIWGPEGRWNDSPCNQSLPSIC
927: CVYMTASREDWGDQRCLTALPYIC
Total matches: 3
Matching pattern PS00213 LIPOCALIN:
1219: GCTYVDVDGAWRTT
Total matches: 1
Matching pattern PS00241 RECEPTOR_CYTOKINES_1:
521: CRKGWTWHSPSCYW
Total matches: 1
Total no of hits in this sequence: 97
========================================
1314 pattern(s) searched in 1 sequence(s), 1479 residues.
Total no of hits in all sequences: 97.
Search time: 00:00 min
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
Start with Profile Search
L=0 25.1908 1951 pos. 244 - 360 PS50041|C_TYPE_LECTIN_2 C-type lectin domain profile.
#
# P 1 YGGHCYYFSte-rktwaeAELFCqQQrpggHlLVSIQSqeeqefLTSLVKasnsyVWIGL -59
# S 244 LTDSCYQFNFQsTLSWREAWASCEQQ--GAD-LLSITEIHEQTYINGLLTGYSSTLWIGL -1180
#
# P 60 NDIttegkWTWSDGtpvSYTNWapgepnnkgerEDCVELys---gKWNDENCEKKLPFVC -2
# S 301 NDLDTSGGWQWSDNSPLKYLNWESDQPDN-PSEENCGVIRTessGGWQNRDCSIALPYVC -1121
#
# P 117 K -1
# S 360 K -1120
#
L=0 28.3140 2195 pos. 389 - 505 PS50041|C_TYPE_LECTIN_2 C-type lectin domain profile.
#
# P 1 YGGHCYYFSterktwaeAELFCqQQrpggHlLVSIQSqeeqefLTSLVKasnsyVWIGLN -58
# S 389 FQGHCYRLQAEKRSWQESKKACLRG--GGD-LVSIHSMAELEFITKQIKQEVEELWIGLN -1035
#
# P 61 DIttegkWTWSDGtpvSYTNWapgepnn-kgerEDCVELys--gKWNDENCEKKLPFVCK -1
# S 446 DLKLQMNFEWSDGSLVSFTHWHPFEPNNfRDSLEDCVTIWGpeGRWNDSPCNQSLPSICK -975
#
L=0 19.7636 1527 pos. 528 - 635 PS50041|C_TYPE_LECTIN_2 C-type lectin domain profile.
#
# P 1 YGGHCYYFSterktwaeAELFCqQQrpggHlLVSIQSqeeqefLTSLVKasn-syVWIGL -59
# S 528 HSPSCYWLGEDQVTYSEARRLCTDH---GSQLVTITNRFEQAFVSSLIYNWEgEYFWTAL -896
#
# P 60 NDIttegkWTWSDGtpvSYTNWapgepnnkgerEDCVELys----gKWNDENC -10
# S 585 QDLNSTGSFFWLSGDEVMYTHWNRDQPG--YSRGGCVALATgsamGLWEVKNC -845
#
L=0 18.4324 1423 pos. 678 - 809 PS50041|C_TYPE_LECTIN_2 C-type lectin domain profile.
#
# P 1 YGGHCYYFSte-----rktwaeAELFCqQQrpggHlLVSIQSqeeqefLTSLVKasn--- -66
# S 678 KLRYCYKVFSSerlqdKKSWVQAQGACQEL--GAQ-LLSLASYEEEHFVANMLNKIFges -746
#
# P 52 -------syVWIGLNDItt--egkWTWSDGtpvSYTNWapgepnnkgerEDCVELys--g -17
# S 735 epeiheqHWFWIGLNRRDPrgGQSWRWSDGVGFSYHNFDRSR-HDDDDIRGCAVLDLasL -687
#
# P 102 KWNDENCEKKLPFVCK -1
# S 794 QWVAMQCDTQLDWICK -671
#
L=0 23.9364 1853 pos. 832 - 951 PS50041|C_TYPE_LECTIN_2 C-type lectin domain profile.
#
# P 1 YGGHCYYFSterktwaeAELFCqQQrpggHlLVSIQSqeeqefLTSLVKasn----syVW -62
# S 832 FQEAEYKFFEHHSTWAQAQRICTWFQ--AE-LTSVHSQAELDFLSHNLQKFSraqeQHWW -592
#
# P 57 IGLNDIttegkWTWSDGtpvSYTNWapgepnnkgerEDCVELys--gKWNDENCEKKLPF -4
# S 889 IGLHTSESDGRFRWTDGSIINFISWAPGKPRPVGKDKKCVYMTAsrEDWGDQRCLTALPY -532
#
# P 115 VCK -1
# S 949 ICK -529
#
L=0 19.8020 1530 pos. 979 - 1101 PS50041|C_TYPE_LECTIN_2 C-type lectin domain profile.
#
# P 1 YGGHCYYFSte----rktwaeAELFCqQQrpggHlLVSIQSqeeqefLTSLVKasnsyVW -62
# S 979 FLNKCFQVQGQepqsRVKWSEAQFSCEQQE--AQ-LVTITNPLEQAFITASLPNVTFDLW -445
#
# P 57 IGLNDIttegkWTWSDGtpvSYTNWapgepnn------kgerEDCVELys-------gKW -15
# S 1036 IGLHAS--QRDFQWVEQEPLMYANWAPGEPSGpspapsGNKPTSCAVVLHspsahftGRW -387
#
# P 104 NDENCEKK -7
# S 1094 DDRSCTEE -379
#
L=0 16.8580 1300 pos. 1132 - 1238 PS50041|C_TYPE_LECTIN_2 C-type lectin domain profile.
#
# P 1 YGGHCYYFSterktwaeAELFCqQQrpggHlLVSIQSqeeqefLTSLVKasnsyVWIGLN -58
# S 1132 YLNGTFRLLQKPLRWHDALLLCESHNAS---LAYVPDPYTQAFLTQAARGLRTPLWIGLA -292
#
# P 61 DIttegkWTWSDGtpvSYTNWapgepnnkgerEDCVELys-gKWNDENCEKKL -6
# S 1189 GEEGSRRYSWVSEEPLNYVGWQDGEPQ---QPGGCTYVDVdGAWRTTSCDTKL -242
#
L=0 16.5892 1279 pos. 1273 - 1384 PS50041|C_TYPE_LECTIN_2 C-type lectin domain profile.
#
# P 1 YGGHCYYFSte-rktwaeAELFCqQQrpggHlLVSIQSqeeqefLTSLVKasn---syVW -62
# S 1273 FREHCYSFHMElLLGHKEARQRCQRA--GGA-VLSILDEMENVFVWEHLQSYEgqsRGAW -151
#
# P 57 IGLNDIttegkWTWSDGtpvSYTNWap---gepnnkgerEDCVELys--gKWNDENC -10
# S 1330 LGMNFNPKGGTLVWQDNTAVNYSNWGPpglG-P-SMLSHNSCYWIQSnsGLWRPGAC -96
#
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
Start with motif search against own library
***** bioMotif : Version V41a DB, 1999 Nov 11 *****
argv[1]=P
argv[2]=-m /data/patterns/own/motif.fa
argv[4]=-seq tem22
***** bioMotif : Version V41a DB, 1999 Nov 11 *****
SeqTyp=2 : PROTEIN search;
>APC D-Box is the MOTIF name
>STATISTICS Total : 0 solutions in 0 sequences, 0 units; out of 1 sequences, 1479 units
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~ ~~~
Start with HMM-search search against own library
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file: /data/patterns/own/own-hmm.lib
Sequence file: tem22
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: Tem22_gi|3327232|dbj|BAA31684.1| KIAA0709 protein [Homo sapiens]
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
[no hits above thresholds]
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
[no hits above thresholds]
Alignments of top-scoring domains:
[no hits above thresholds]
//
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file: /data/patterns/own/own-hmm-f.lib
Sequence file: tem22
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: Tem22_gi|3327232|dbj|BAA31684.1| KIAA0709 protein [Homo sapiens]
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
[no hits above thresholds]
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
[no hits above thresholds]
Alignments of top-scoring domains:
[no hits above thresholds]
//
~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~ ~~~~~
L. Aravind's signalling DB
IMPALA version 1.1 [20-December-1999]
Reference: Alejandro A. Schaffer, Yuri I. Wolf, Chris P. Ponting,
Eugene V. Koonin, L. Aravind, Stephen F. Altschul (1999),
"IMPALA: Matching a Protein Sequence Against a Collection of
"PSI-BLAST-Constructed Position-Specific Score Matrices",
Bioinformatics 15:1000-1011.
Query= Tem22_gi|3327232|dbj|BAA31684.1| KIAA0709 protein [Homo
sapiens]
(1479 letters)
Searching..................................done
Results from profile search
Score E
Sequences producing significant alignments: (bits) Value
HISDAC Histone deacetylase domain 29 0.044
UBA Ubiquitin pathway associated domain 27 0.25
RHOGEF RHO-type GTPase GDP exchange factor 24 1.2
CALC Calcineurin like Phosphoesterase domain 24 1.3
DHHC Novel zinc finger domain with DHHC signature 24 1.4
SPRY SPRY domain 24 2.0
SNARE Alpha helical domains which are involved in vesicle fu... 23 2.4
PH Pleckstrin homology domain (lipid and protein interactio... 23 3.4
AAA AAA+ ATPase Module 23 4.4
GAF GAF domain (Predicted Small-ligand binding domain) 22 4.6
KIN Protein kinase domain 22 5.6
AP2 A plant specific DNA binding domain (Apetala 2 like) 22 6.3
KR Kringle domain (Adhesion module) 21 7.8
>HISDAC Histone deacetylase domain
Length = 433
Score = 29.2 bits (65), Expect = 0.044
Identities = 17/78 (21%), Positives = 29/78 (36%), Gaps = 20/78 (25%)
Query: 858 AELTSVHSQAELDFLSH----NLQKFSRAQEQHWWIGLHTSESDGRF----RWTDGSI-- 907
E+ H+ +DFLS NL+ F R + + +G DG + GS+
Sbjct: 72 QEMCQFHTDEYIDFLSRVTPDNLEMFKRESVK-FNVGDDCPVFDGLYEYCSISGGGSMEG 130
Query: 908 ---------INFISWAPG 916
+++A G
Sbjct: 131 AARLNRGKCDVAVNYAGG 148
Score = 22.2 bits (47), Expect = 4.6
Identities = 5/18 (27%), Positives = 8/18 (43%)
Query: 271 ADLLSITEIHEQTYINGL 288
A + + H YI+ L
Sbjct: 69 ATKQEMCQFHTDEYIDFL 86
>UBA Ubiquitin pathway associated domain
Length = 255
Score = 26.6 bits (58), Expect = 0.25
Identities = 13/61 (21%), Positives = 22/61 (35%), Gaps = 9/61 (14%)
Query: 689 ERLQDKKSWVQA-----QGACQELGA---QLLSLASYEEEHFVANMLNKIFGESEPEIHE 740
E L + V + + A QL E FV +ML + G++ ++ E
Sbjct: 133 EDLLSLRQVVSGNPEALAPLLENISARYPQLREHIMANPEVFV-SMLLEAVGDNMQDVME 191
Query: 741 Q 741
Sbjct: 192 G 192
>RHOGEF RHO-type GTPase GDP exchange factor
Length = 176
Score = 24.1 bits (52), Expect = 1.2
Identities = 9/34 (26%), Positives = 14/34 (40%), Gaps = 1/34 (2%)
Query: 1278 YSFHMELLLGHKEAR-QRCQRAGGAVLSILDEME 1310
Y FH ++ L E +R G L D+ +
Sbjct: 55 YEFHNDIFLSSLENCAHAPERVGPCFLERKDDFQ 88
>CALC Calcineurin like Phosphoesterase domain
Length = 274
Score = 24.0 bits (51), Expect = 1.3
Identities = 8/54 (14%), Positives = 14/54 (25%), Gaps = 2/54 (3%)
Query: 150 GDQTRSGQWRIYGSEEDLCALPYHEVYTIQGNSHGKPCT--IPFKYDNQWFHGC 201
GD + Y ++ V+ I GN +P +
Sbjct: 62 GDLVQDSSDEGYIRFVEMMKPFNKPVFWIPGNHDFQPKMAEFLNQPPMNAAKHL 115
>DHHC Novel zinc finger domain with DHHC signature
Length = 217
Score = 24.0 bits (51), Expect = 1.4
Identities = 9/16 (56%), Positives = 11/16 (68%)
Query: 1059 APGEPSGPSPAPSGNK 1074
AP +PSGPSP G +
Sbjct: 23 APAQPSGPSPELQGQR 38
Score = 21.6 bits (45), Expect = 7.0
Identities = 4/48 (8%), Positives = 5/48 (10%), Gaps = 2/48 (4%)
Query: 184 GKP-CTIPFKYDNQWFHGCTSTGREDGHLWCATTQDYGKDERWGFCPI 230
+ L C R C
Sbjct: 106 ADDNVRDKSYAGPLPIFNRSQHAHVIEDLHCNLCNVD-VSARSKHCSA 152
>SPRY SPRY domain
Length = 122
Score = 23.7 bits (51), Expect = 2.0
Identities = 16/87 (18%), Positives = 23/87 (26%), Gaps = 11/87 (12%)
Query: 518 DHGCRKGW-TWHSPSCYWLGEDQVTYS-EARRLCTDHGSQLVTITNRFEQAFVSSLIYNW 575
G T P + G Q +Y C ++ F V Y+
Sbjct: 15 GDKICFGLTTNDHPIEVYPGNYQGSYGYSGDGKCYFGTNEGRVYGPSFSSGDVVGCGYDS 74
Query: 576 E---------GEYFWTALQDLNSTGSF 593
G Y A Q +N G +
Sbjct: 75 SSKTLYFTKNGVYLGVAAQKVNLIGLY 101
>SNARE Alpha helical domains which are involved in vesicle fusion
Length = 254
Score = 23.3 bits (50), Expect = 2.4
Identities = 15/62 (24%), Positives = 22/62 (35%), Gaps = 9/62 (14%)
Query: 1391 VVCKLPRAEQSS--FSPSA-------LPENPAALVVVLMAVLLLLALLTAALILYRRRQS 1441
+C+L A + FS + V +L+LL AL L R +
Sbjct: 154 PMCELGSANGVTSAFSVDCKGAGKISSAHQRLEPATLSGIVAFILSLLCGALNLIRGFHA 213
Query: 1442 IE 1443
IE
Sbjct: 214 IE 215
>PH Pleckstrin homology domain (lipid and protein interaction domain)
Length = 138
Score = 22.7 bits (48), Expect = 3.4
Identities = 8/73 (10%), Positives = 24/73 (31%), Gaps = 4/73 (5%)
Query: 628 GLWEVKNCTSFRARYICRQSLGTPVTPELPGPDPTPSLTGSCPQGWASDTKLRYCYKVFS 687
G+ ++N + + + P G T + + + + S
Sbjct: 56 GIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGNHMVYR----IS 111
Query: 688 SERLQDKKSWVQA 700
+ ++K W+++
Sbjct: 112 APTQEEKDEWIKS 124
>AAA AAA+ ATPase Module
Length = 298
Score = 22.5 bits (47), Expect = 4.4
Identities = 6/13 (46%), Positives = 10/13 (76%)
Query: 1354 WGPPGLGPSMLSH 1366
+GPPG G ++L+
Sbjct: 86 YGPPGTGKTLLAR 98
>GAF GAF domain (Predicted Small-ligand binding domain)
Length = 160
Score = 22.2 bits (47), Expect = 4.6
Identities = 9/41 (21%), Positives = 19/41 (45%)
Query: 701 QGACQELGAQLLSLASYEEEHFVANMLNKIFGESEPEIHEQ 741
+ C L A +SL Y + +A + ++F + + E+
Sbjct: 11 KKLCFLLQADRMSLFMYRARNGIAELATRLFNVHKDAVLEE 51
>KIN Protein kinase domain
Length = 313
Score = 22.0 bits (46), Expect = 5.6
Identities = 12/70 (17%), Positives = 20/70 (28%), Gaps = 6/70 (8%)
Query: 927 CVYMTASREDWGDQRCLTALPYICKRSNVTKETQPPDLPTTALGGCPSDWIQFLNKCFQV 986
+ S ED + A +I ++ QP D I L +
Sbjct: 248 ETIGSPSEEDICNIANEQARQFI---RSLNMGNQPKVNFANMFPKANPDAIDLLERMLYF 304
Query: 987 QGQEPQSRVK 996
+P R+
Sbjct: 305 ---DPSKRLT 311
>AP2 A plant specific DNA binding domain (Apetala 2 like)
Length = 218
Score = 22.0 bits (46), Expect = 6.3
Identities = 12/47 (25%), Positives = 19/47 (39%), Gaps = 6/47 (12%)
Query: 75 QRW-KWVSRNRLFNLGTMQCLGTGWPGTNTTASLGMYECDREALNLR 120
+ W KWVS R + W GT + + D AL+++
Sbjct: 42 RNWGKWVSEIREPRKKSRI-----WLGTFPSPEMAARAHDVAALSIK 83
>KR Kringle domain (Adhesion module)
Length = 86
Score = 21.5 bits (45), Expect = 7.8
Identities = 8/30 (26%), Positives = 14/30 (46%), Gaps = 4/30 (13%)
Query: 208 DGHLWCATTQDYGKDERWGFCPIKSNDCET 237
D WC +D W +C +++ CE+
Sbjct: 61 DERPWCYVVKDSAL--SWEYCRLEA--CES 86
Underlying Matrix: BLOSUM62
Number of sequences tested against query: 105
Number of sequences better than 10.0: 13
Number of calls to ALIGN: 15
Length of query: 1479
Total length of test sequences: 20182
Effective length of test sequences: 16637.0
Effective search space size: 24045297.3
Initial X dropoff for ALIGN: 25.0 bits
Y. Wolf's SCOP PSSM
IMPALA version 1.1 [20-December-1999]
Reference: Alejandro A. Schaffer, Yuri I. Wolf, Chris P. Ponting,
Eugene V. Koonin, L. Aravind, Stephen F. Altschul (1999),
"IMPALA: Matching a Protein Sequence Against a Collection of
"PSI-BLAST-Constructed Position-Specific Score Matrices",
Bioinformatics 15:1000-1011.
Query= Tem22_gi|3327232|dbj|BAA31684.1| KIAA0709 protein [Homo
sapiens]
(1479 letters)
Searching.................................................done
Results from profile search
Score E
Sequences producing significant alignments: (bits) Value
gi|1469029 [26..148] C-type lectin-like 111 9e-26
gi|1053218 [103..244] C-type lectin-like 103 2e-23
gi|1078939 [54..180] C-type lectin-like 103 2e-23
gi|1109918 [24..147] C-type lectin-like 102 4e-23
gi|2706599 [57..185] C-type lectin-like 100 2e-22
gi|2502062 [25..167] C-type lectin-like 93 4e-20
gi|82035 [136..263] beta-Trefoil 44 1e-05
gi|1655439 [28..139] C-type lectin-like 37 0.002
gi|1685117 [55..161] C-type lectin-like 37 0.002
gi|438998 [1..297] S-adenosyl-L-methionine-dependent methylt... 29 0.73
gi|2507361 [53..298] Periplasmic binding protein-like I 28 0.87
gi|115715 [1..97] Ferredoxin-like 28 1.2
gi|2500689 [29..252] Periplasmic binding protein-like I 27 1.8
gi|1536958 [63..316] Periplasmic binding protein-like I 27 2.0
gi|2650282 [2..154] P-loop containing nucleotide triphosphat... 27 2.1
gi|1708629 [67..339] Periplasmic binding protein-like I 27 2.5
gi|797337 [17..308] Periplasmic binding protein-like I 26 3.7
gi|729214 [63..305] Periplasmic binding protein-like I 26 5.4
gi|2244909 [587..959] beta/alpha (TIM)-barrel 26 5.6
gi|137196 [30..266] P-loop containing nucleotide triphosphat... 25 7.2
gi|586847 [59..303] Periplasmic binding protein-like I 25 7.5
gi|628933 [59..301] Periplasmic binding protein-like I 25 7.6
gi|2500694 [73..586] Periplasmic binding protein-like II 25 8.3
gi|121769 [3..207] Class I glutamine amidotransferases 25 8.5
gi|1616941 [10..247] Metzincin-like 25 9.0
gi|1762781 [797..951] C2 domain-like 25 9.0
gi|2506563 [66..342] Periplasmic binding protein-like I 25 9.5
>gi|1469029 [26..148] C-type lectin-like
Length = 123
Score = 111 bits (275), Expect = 9e-26
Identities = 25/127 (19%), Positives = 44/127 (33%), Gaps = 7/127 (5%)
Query: 382 CEPSWQPFQ--GHCYRLQAEKRSWQESKKACLRGGGDLVSIHSMAELEFITKQIKQEVEE 439
C W +CY ++ E+ C GG + + E F+ + +
Sbjct: 1 CPDGWLLSNETSYCYLTSDRYMTFAEADGYCQSLGGSQAFMMNSYEFTFMKSYTQGMFAQ 60
Query: 440 LWIGLNDLKLQMNFEWSDGSLVSFTHWHPFEPNNFRDSLEDCVTIW-GPEGRWNDSPCNQ 498
W+G+ + +DG+ + W EP+ DCV+ + + C
Sbjct: 61 PWLGVTRNLTNNKWYNNDGTTPYSSWWIAGEPSL----NGDCVSFKSTDKQGIRATQCWS 116
Query: 499 SLPSICK 505
P ICK
Sbjct: 117 IQPVICK 123
Score = 107 bits (265), Expect = 1e-24
Identities = 25/126 (19%), Positives = 46/126 (35%), Gaps = 3/126 (2%)
Query: 235 CETFWDKDQLTDSCYQFNFQSTLSWREAWASCEQQGADLLSITEIHEQTYINGLLTGYSS 294
C W T CY +++ EA C+ G + +E T++ G +
Sbjct: 1 CPDGWLLSNETSYCYLT-SDRYMTFAEADGYCQSLGGSQAFMMNSYEFTFMKSYTQGMFA 59
Query: 295 TLWIGLNDLDTSGGWQWSDNSPLKYLNWESDQPDNPSEENCGVIRTESSGGWQNRDCSIA 354
W+G+ T+ W +D + W + +P +C ++ G + C
Sbjct: 60 QPWLGVTRNLTNNKWYNNDGTTPYSSWWIAGEPSL--NGDCVSFKSTDKQGIRATQCWSI 117
Query: 355 LPYVCK 360
P +CK
Sbjct: 118 QPVICK 123
Score = 87.5 bits (214), Expect = 1e-18
Identities = 25/126 (19%), Positives = 50/126 (38%), Gaps = 10/126 (7%)
Query: 829 WLRFQEAE--YKFFEHHSTWAQAQRICTWFQAELTSVHSQAELDFLSHNLQKFSRAQEQH 886
WL E Y + + T+A+A C + + E F ++ +++
Sbjct: 5 WLLSNETSYCYLTSDRYMTFAEADGYCQSLGGSQAFMMNSYEFTF----MKSYTQGMFAQ 60
Query: 887 WWIGLHTSESDGRFRWTDGSIINFISWAPGKPRPVGKDKKCVYMTASRED-WGDQRCLTA 945
W+G+ + ++ ++ DG+ W G+P G CV ++ + +C +
Sbjct: 61 PWLGVTRNLTNNKWYNNDGTTPYSSWWIAGEPSLNG---DCVSFKSTDKQGIRATQCWSI 117
Query: 946 LPYICK 951
P ICK
Sbjct: 118 QPVICK 123
Score = 84.4 bits (206), Expect = 1e-17
Identities = 25/126 (19%), Positives = 43/126 (33%), Gaps = 6/126 (4%)
Query: 521 CRKGWTWHSP--SCYWLGEDQVTYSEARRLCTDHGSQLVTITNRFEQAFVSSLIYNWEGE 578
C GW + CY + +T++EA C G + N +E F+ S
Sbjct: 1 CPDGWLLSNETSYCYLTSDRYMTFAEADGYCQSLGGSQAFMMNSYEFTFMKSYT-QGMFA 59
Query: 579 YFWTALQDLNSTGSFFWLSGDEVMYTHWNRDQPGYSRGGCVALATGSAMGLWEVKNCTSF 638
W + + ++ G + W +P G CV+ + + C S
Sbjct: 60 QPWLGVTRNLTNNKWYNNDGTTPYSSWWIAGEP-SLNGDCVSFKS-TDKQGIRATQCWSI 117
Query: 639 RARYIC 644
+ IC
Sbjct: 118 Q-PVIC 122
Score = 84.0 bits (205), Expect = 1e-17
Identities = 20/141 (14%), Positives = 41/141 (28%), Gaps = 22/141 (15%)
Query: 972 CPSDWIQF--LNKCFQVQGQEPQSRVKWSEAQFSCEQQEAQLVTITNPLEQAFITASLPN 1029
CP W+ + C+ + + ++EA C+ + N E F+ +
Sbjct: 1 CPDGWLLSNETSYCYLTSDR----YMTFAEADGYCQSLGGSQAFMMNSYEFTFMKSYTQG 56
Query: 1030 VTFDLWIGLHA--SQRDFQWVEQEPLMYANWAPGEPSGPSPAPSGNKPTSCAVVLHSPSA 1087
+ W+G+ + + + + W GEP + C +
Sbjct: 57 MFAQPWLGVTRNLTNNKWYNNDGTTPYSSWWIAGEP---------SLNGDCVSFKSTD-- 105
Query: 1088 HFTGRWDDRSCTEETHGFICQ 1108
C IC+
Sbjct: 106 --KQGIRATQCWSI-QPVICK 123
Score = 78.2 bits (190), Expect = 8e-16
Identities = 29/142 (20%), Positives = 44/142 (30%), Gaps = 20/142 (14%)
Query: 669 CPQGWASDTKLRYCYKVFSSERLQDKKSWVQAQGACQELGAQLLSLASYEEEHFVANMLN 728
CP GW + YCY ++ +A G CQ LG + + E F+ +
Sbjct: 1 CPDGWLLSNETSYCYLTS-----DRYMTFAEADGYCQSLGGSQAFMMNSYEFTFMKSYTQ 55
Query: 729 KIFGESEPEIHEQHWFWIGLNRRDPRGGQSWRWSDGVGFSYHNFDRSRHDDDDIRGCAVL 788
+F W+G+ R W +DG + + C
Sbjct: 56 GMFA----------QPWLGVTRNLTNN--KWYNNDGTTPYSSWWIAG--EPSLNGDCVSF 101
Query: 789 DLA-SLQWVAMQCDTQLDWICK 809
A QC + ICK
Sbjct: 102 KSTDKQGIRATQCWSIQPVICK 123
Score = 65.3 bits (157), Expect = 6e-12
Identities = 20/118 (16%), Positives = 41/118 (33%), Gaps = 3/118 (2%)
Query: 1128 TELSYLNGTFRLLQKPLRWHDALLLCESHNASLAYVPDPYTQAFLTQAARGLRTPLWIGL 1187
+ + + + + + +A C+S S A++ + Y F+ +G+ W+G+
Sbjct: 6 LLSNETSYCYLTSDRYMTFAEADGYCQSLGGSQAFMMNSYEFTFMKSYTQGMFAQPWLGV 65
Query: 1188 AGEEGSRRYSWVSEEPLNYVGWQDGEPQQPGGCTYVDVD--GAWRTTSCDTKLQGAVC 1243
+ ++ W GEP G C R T C + +C
Sbjct: 66 TRNLTNNKWYNNDGTTPYSSWWIAGEPSLNGDCVSFKSTDKQGIRATQCWSIQ-PVIC 122
Score = 64.9 bits (156), Expect = 8e-12
Identities = 32/136 (23%), Positives = 49/136 (35%), Gaps = 15/136 (11%)
Query: 1261 CPQGLADSAWIPFRE--HCYSFHMELLLGHKEARQRCQRAGGAVLSILDEMENVFVWEHL 1318
CP G W+ E +CY + EA CQ GG+ +++ E F+ +
Sbjct: 1 CPDG-----WLLSNETSYCYLTSDR-YMTFAEADGYCQSLGGSQAFMMNSYEFTFMKSYT 54
Query: 1319 QSYEGQSRGAWLGMNFNPKGGTLVWQDNTAVNYSNWGPPGLGPSMLSHNSCYWIQSNSGL 1378
Q Q WLG+ N D T S W G L+ + + ++
Sbjct: 55 QGMFAQ---PWLGVTRNLTNNKWYNNDGTTPYSSWWIA---GEPSLNGDCVSFKSTDKQG 108
Query: 1379 WRPGACTNITMGVVCK 1394
R C + V+CK
Sbjct: 109 IRATQCWS-IQPVICK 123
>gi|1053218 [103..244] C-type lectin-like
Length = 142
Score = 103 bits (256), Expect = 2e-23
Identities = 26/142 (18%), Positives = 49/142 (34%), Gaps = 18/142 (12%)
Query: 382 CEPSWQPFQGHCYRLQAEKRSWQESKKACLR-GGGDLVSIHSMAELEFITKQIKQEVEEL 440
C + + G CY R+W ++ C L + S + F ++ +
Sbjct: 1 CPKNSIYYMGSCYVFDTTLRNWNDAALYCNNMNSATLPLVESAEDQAFFAGYLQAMIPSN 60
Query: 441 WIGL--------------NDLKLQMNFEWSDGS-LVSFTHWHPFEPNNFRDSLEDCVTIW 485
+ + ++ + GS LV+ T W P EPN + + + CV +
Sbjct: 61 PPADMRPPPDGIWTAVRGVNNVTRASWVYYPGSFLVTDTFWAPQEPNIYVNYNDVCVALQ 120
Query: 486 --GPEGRWNDSPCNQSLPSICK 505
W + C ++CK
Sbjct: 121 SDSFYREWTTALCTILKYTVCK 142
Score = 80.9 bits (197), Expect = 1e-16
Identities = 27/145 (18%), Positives = 44/145 (29%), Gaps = 22/145 (15%)
Query: 235 CETFWDKDQLTDSCYQFNFQSTLSWREAWASCEQ-QGADLLSITEIHEQTYINGLLTGYS 293
C SCY F + +W +A C A L + +Q + G L
Sbjct: 1 CPKNS--IYYMGSCYVF-DTTLRNWNDAALYCNNMNSATLPLVESAEDQAFFAGYLQAMI 57
Query: 294 STLWIGL--------------NDLDTSGGWQWSDNSPLK-YLNWESDQPDNP--SEENCG 336
+ + T W + S L W +P+ + C
Sbjct: 58 PSNPPADMRPPPDGIWTAVRGVNNVTRASWVYYPGSFLVTDTFWAPQEPNIYVNYNDVCV 117
Query: 337 VIRTESS-GGWQNRDCSIALPYVCK 360
++++S W C+I VCK
Sbjct: 118 ALQSDSFYREWTTALCTILKYTVCK 142
Score = 76.7 bits (186), Expect = 3e-15
Identities = 25/138 (18%), Positives = 42/138 (30%), Gaps = 15/138 (10%)
Query: 829 WLRFQEAEYKFFEHHSTWAQAQRICT-WFQAELTSVHSQAELDFL---------SHNLQK 878
+ + + Y F W A C A L V S + F S+
Sbjct: 5 SIYYMGSCYVFDTTLRNWNDAALYCNNMNSATLPLVESAEDQAFFAGYLQAMIPSNPPAD 64
Query: 879 FSRAQEQHW-WIGLHTSESDGRFRWTDGSIIN-FISWAPGKPRP-VGKDKKCVYMTASRE 935
+ W + + + + + GS + WAP +P V + CV + +
Sbjct: 65 MRPPPDGIWTAVRGVNNVTRASWVYYPGSFLVTDTFWAPQEPNIYVNYNDVCVALQSDSF 124
Query: 936 D--WGDQRCLTALPYICK 951
W C +CK
Sbjct: 125 YREWTTALCTILKYTVCK 142
Score = 75.5 bits (183), Expect = 5e-15
Identities = 30/142 (21%), Positives = 52/142 (36%), Gaps = 19/142 (13%)
Query: 521 CRKGWTWHSPSCYWLGEDQVTYSEARRLCTDH-GSQLVTITNRFEQAFVSSLI-----YN 574
C K ++ SCY +++A C + + L + + +QAF + + N
Sbjct: 1 CPKNSIYYMGSCYVFDTTLRNWNDAALYCNNMNSATLPLVESAEDQAFFAGYLQAMIPSN 60
Query: 575 WEG------EYFWTAL--QDLNSTGSFFWLSGDE-VMYTHWNRDQPGY---SRGGCVALA 622
+ WTA+ + + S+ + G V T W +P CVAL
Sbjct: 61 PPADMRPPPDGIWTAVRGVNNVTRASWVYYPGSFLVTDTFWAPQEPNIYVNYNDVCVALQ 120
Query: 623 TGSAMGLWEVKNCTSFRARYIC 644
+ S W CT + +C
Sbjct: 121 SDSFYREWTTALCTILK-YTVC 141
Score = 70.0 bits (169), Expect = 3e-13
Identities = 26/149 (17%), Positives = 43/149 (28%), Gaps = 15/149 (10%)
Query: 669 CPQGWASDTKLRYCYKVFSSERLQDKKSWVQAQGACQEL-GAQLLSLASYEEEHFVANML 727
CP+ CY ++W A C + A L + S E++ F A L
Sbjct: 1 CPKNSIYYM--GSCYVFD-----TTLRNWNDAALYCNNMNSATLPLVESAEDQAFFAGYL 53
Query: 728 NKIFGESEPEIHE--QHWFWIGLNRRDPRGGQSWRWSDGVGF-SYHNFDRSRHDDDDIRG 784
+ + P W + + SW + G + + +
Sbjct: 54 QAMIPSNPPADMRPPPDGIWTAVRGVNNVTRASWVYYPGSFLVTDTFWAPQEPNIYVNYN 113
Query: 785 --CAVLDLASL--QWVAMQCDTQLDWICK 809
C L S +W C +CK
Sbjct: 114 DVCVALQSDSFYREWTTALCTILKYTVCK 142
Score = 68.1 bits (164), Expect = 8e-13
Identities = 26/155 (16%), Positives = 46/155 (28%), Gaps = 31/155 (20%)
Query: 972 CPSDWIQFLNKCFQVQGQEPQSRVKWSEAQFSCEQQE-AQLVTITNPLEQAFITASLPNV 1030
CP + I ++ C+ W++A C A L + + +QAF L +
Sbjct: 1 CPKNSIYYMGSCYVFDTT----LRNWNDAALYCNNMNSATLPLVESAEDQAFFAGYLQAM 56
Query: 1031 TF------------DLWIGLH----ASQRDFQWVEQEPL-MYANWAPGEPSGPSPAPSGN 1073
+W + ++ + + L WAP EP+ N
Sbjct: 57 IPSNPPADMRPPPDGIWTAVRGVNNVTRASWVYYPGSFLVTDTFWAPQEPNIYV-----N 111
Query: 1074 KPTSCAVVLHSPSAHFTGRWDDRSCTEETHGFICQ 1108
C + W CT +C+
Sbjct: 112 YNDVCVALQSDSFY---REWTTALCTIL-KYTVCK 142
Score = 56.8 bits (135), Expect = 3e-09
Identities = 29/149 (19%), Positives = 48/149 (31%), Gaps = 22/149 (14%)
Query: 1261 CPQGLADSAWIPFREHCYSFHMELLLGHKEARQRCQRAGGAVL-SILDEMENVFVWEHLQ 1319
CP+ I + CY F L +A C A L + + F +LQ
Sbjct: 1 CPKN-----SIYYMGSCYVFDTT-LRNWNDAALYCNNMNSATLPLVESAEDQAFFAGYLQ 54
Query: 1320 SYEGQSR---------GAWLGMNFNPKG--GTLVWQDNTAVN-YSNWGPPGLGPSMLSHN 1367
+ + G W + + V+ + + + W P + ++
Sbjct: 55 AMIPSNPPADMRPPPDGIWTAVRGVNNVTRASWVYYPGSFLVTDTFWAPQEPNIYVNYND 114
Query: 1368 SCYWIQSNS--GLWRPGACTNITMGVVCK 1394
C +QS+S W CT VCK
Sbjct: 115 VCVALQSDSFYREWTTALCTI-LKYTVCK 142
Score = 42.0 bits (97), Expect = 7e-05
Identities = 23/130 (17%), Positives = 32/130 (23%), Gaps = 24/130 (18%)
Query: 1137 FRLLQKPLRWHDALLLCESHN-ASLAYVPDPYTQAFLTQAARGLRTPLWIGL-------- 1187
+ W+DA L C + N A+L V QAF + +
Sbjct: 13 YVFDTTLRNWNDAALYCNNMNSATLPLVESAEDQAFFAGYLQAMIPSNPPADMRPPPDGI 72
Query: 1188 ------AGEEGSRRYSWVSEEPLN-YVGWQDGEPQ----QPGGCTYVDVD---GAWRTTS 1233
+ + L W EP C + D W T
Sbjct: 73 WTAVRGVNNVTRASWVYYPGSFLVTDTFWAPQEPNIYVNYNDVCVALQSDSFYREWTTAL 132
Query: 1234 CDTKLQGAVC 1243
C VC
Sbjct: 133 CTILK-YTVC 141
>gi|1078939 [54..180] C-type lectin-like
Length = 127
Score = 103 bits (256), Expect = 2e-23
Identities = 27/129 (20%), Positives = 51/129 (38%), Gaps = 8/129 (6%)
Query: 382 CEPSWQPFQGHCYRLQA--EKRSWQESKKACLRGGGDLVSIHSMAELEFITKQIKQ-EVE 438
C W+ CY + A + +S ++ AC + G+L E F+ K +++ +
Sbjct: 1 CNEGWRGAFNFCYYISAFADIQSHSGAQAACKQQKGELFWPDLPYESFFLKKVLQRVKTS 60
Query: 439 ELWIGLNDLKLQMNFEWSDGS-LVSFTHWHPFEPNNFRDSLEDCVTIWGPEGRWNDSPCN 497
++ N K + W G + W P +P+ +C+ ++ G +D C
Sbjct: 61 TNFVWTNGEKHNGQWNWGTGHPAFTAPRWSPGQPDG----QGECLAVYTHSGFLDDKQCE 116
Query: 498 QSLPSICKK 506
ICK
Sbjct: 117 TQYNYICKT 125
Score = 89.9 bits (220), Expect = 3e-19
Identities = 34/131 (25%), Positives = 52/131 (38%), Gaps = 8/131 (6%)
Query: 235 CETFWDKDQLTDSCYQFN-FQSTLSWREAWASCEQQGADLLSITEIHEQTYINGLLTGY- 292
C W + CY + F S A A+C+QQ +L +E ++ +L
Sbjct: 1 CNEGW--RGAFNFCYYISAFADIQSHSGAQAACKQQKGELFWPDLPYESFFLKKVLQRVK 58
Query: 293 SSTLWIGLNDLDTSGGWQWSDNSPLK-YLNWESDQPDNPSEENCGVIRTESSGGWQNRDC 351
+ST ++ N +G W W P W QPD + C + T SG ++ C
Sbjct: 59 TSTNFVWTNGEKHNGQWNWGTGHPAFTAPRWSPGQPDG--QGECLAVYT-HSGFLDDKQC 115
Query: 352 SIALPYVCKKK 362
Y+CK K
Sbjct: 116 ETQYNYICKTK 126
Score = 84.0 bits (205), Expect = 2e-17
Identities = 32/142 (22%), Positives = 50/142 (34%), Gaps = 21/142 (14%)
Query: 972 CPSDWIQFLNKCFQVQGQEPQSRVKWSEAQFSCEQQEAQLVTITNPLEQAFITASLPNV- 1030
C W N C+ + S AQ +C+QQ+ +L P E F+ L V
Sbjct: 1 CNEGWRGAFNFCYYISAFA--DIQSHSGAQAACKQQKGELFWPDLPYESFFLKKVLQRVK 58
Query: 1031 --TFDLWIGLHASQRDFQWVEQEPLM-YANWAPGEPSGPSPAPSGNKPTSCAVVLHSPSA 1087
T +W + W P W+PG+P + C V
Sbjct: 59 TSTNFVWTNGEKHNGQWNWGTGHPAFTAPRWSPGQP---------DGQGECLAVYTH--- 106
Query: 1088 HFTGRWDDRSCTEETHGFICQK 1109
+G DD+ C + + +IC+
Sbjct: 107 --SGFLDDKQCETQ-YNYICKT 125
Score = 81.7 bits (199), Expect = 7e-17
Identities = 28/129 (21%), Positives = 48/129 (36%), Gaps = 9/129 (6%)
Query: 829 WLRFQEAEYKF--FEHHSTWAQAQRICTWFQAELTSVHSQAELDFLSHNLQKFSRAQEQH 886
W Y F + + AQ C + EL E FL LQ+ +
Sbjct: 5 WRGAFNFCYYISAFADIQSHSGAQAACKQQKGELFWPDLPYESFFLKKVLQRVKTSTN-- 62
Query: 887 WWIGLHTSESDGRFRWTDGS-IINFISWAPGKPRPVGKDKKCVYMTASREDWGDQRCLTA 945
++ + + +G++ W G W+PG+P +C+ + D++C T
Sbjct: 63 -FVWTNGEKHNGQWNWGTGHPAFTAPRWSPGQPD---GQGECLAVYTHSGFLDDKQCETQ 118
Query: 946 LPYICKRSN 954
YICK +
Sbjct: 119 YNYICKTKH 127
Score = 77.4 bits (188), Expect = 1e-15
Identities = 30/142 (21%), Positives = 52/142 (36%), Gaps = 19/142 (13%)
Query: 669 CPQGWASDTKLRYCYKVFSSERLQDKKSWVQAQGACQELGAQLLSLASYEEEHFVANMLN 728
C +GW +CY + + D +S AQ AC++ +L E F+ +L
Sbjct: 1 CNEGWRGAF--NFCYYISAFA---DIQSHSGAQAACKQQKGELFWPDLPYESFFLKKVLQ 55
Query: 729 KIFGESEPEIHEQHWFWIGLNRRDPRGGQSWRWSDGVG-FSYHNFDRSRHDDDDIRGCAV 787
++ ++ W + + W W G F+ + D C
Sbjct: 56 RVK-------TSTNFVWTNGEKHN----GQWNWGTGHPAFTAPRWSPG--QPDGQGECLA 102
Query: 788 LDLASLQWVAMQCDTQLDWICK 809
+ S QC+TQ ++ICK
Sbjct: 103 VYTHSGFLDDKQCETQYNYICK 124
Score = 76.2 bits (185), Expect = 4e-15
Identities = 24/127 (18%), Positives = 41/127 (31%), Gaps = 7/127 (5%)
Query: 521 CRKGWTWHSPSCYW--LGEDQVTYSEARRLCTDHGSQLVTITNRFEQAFVSSLIYNWEGE 578
C +GW CY+ D ++S A+ C +L +E F+ ++ +
Sbjct: 1 CNEGWRGAFNFCYYISAFADIQSHSGAQAACKQQKGELFWPDLPYESFFLKKVLQRVKTS 60
Query: 579 YFWTALQDLNSTGSFFWLSGDEVM-YTHWNRDQPGYSRGGCVALATGSAMGLWEVKNCTS 637
+ G + W +G W+ QP G + K C +
Sbjct: 61 TNFVWTNGEKHNGQWNWGTGHPAFTAPRWSPGQPDGQGECLAVYTH---SGFLDDKQCET 117
Query: 638 FRARYIC 644
YIC
Sbjct: 118 QY-NYIC 123
Score = 67.7 bits (163), Expect = 1e-12
Identities = 25/136 (18%), Positives = 42/136 (30%), Gaps = 14/136 (10%)
Query: 1261 CPQGLADSAWIPFREHCYSFHM-ELLLGHKEARQRCQRAGGAVLSILDEMENVFVWEHLQ 1319
C +G W CY + H A+ C++ G + E+ F+ + LQ
Sbjct: 1 CNEG-----WRGAFNFCYYISAFADIQSHSGAQAACKQQKGELFWPDLPYESFFLKKVLQ 55
Query: 1320 SYEGQSRGAWLGMNFNPKGGTLVWQDNTAV-NYSNWGPPGLGPSMLSHNSCYWIQSNSGL 1378
+ + ++ N G W W P P C + ++SG
Sbjct: 56 RVKTST--NFVWTNGEKHNGQWNWGTGHPAFTAPRWSPG--QPD--GQGECLAVYTHSGF 109
Query: 1379 WRPGACTNITMGVVCK 1394
C +CK
Sbjct: 110 LDDKQCET-QYNYICK 124
Score = 52.5 bits (124), Expect = 5e-08
Identities = 21/112 (18%), Positives = 39/112 (34%), Gaps = 4/112 (3%)
Query: 1136 TFRLLQKPLRWHDALLLCESHNASLAYVPDPYTQAFLTQAARGL-RTPLWIGLAGEEGSR 1194
A C+ L + PY FL + + + + ++ GE+ +
Sbjct: 14 YISAFADIQSHSGAQAACKQQKGELFWPDLPYESFFLKKVLQRVKTSTNFVWTNGEKHNG 73
Query: 1195 RYSWVSEEP-LNYVGWQDGEPQQPGGCTYVDV-DGAWRTTSCDTKLQGAVCG 1244
+++W + P W G+P G C V G C+T+ +C
Sbjct: 74 QWNWGTGHPAFTAPRWSPGQPDGQGECLAVYTHSGFLDDKQCETQY-NYICK 124
>gi|1109918 [24..147] C-type lectin-like
Length = 124
Score = 102 bits (253), Expect = 4e-23
Identities = 25/127 (19%), Positives = 44/127 (33%), Gaps = 7/127 (5%)
Query: 382 CEPSWQPFQGHCYRLQAEKRSWQESKKACLR---GGGDLVSIHSMAELEFITKQIKQEVE 438
C + + CY + + C + L + + AE F+T+Q++
Sbjct: 1 CPTGTKIYNAKCYAALGMDMTRDNALAYCRKTYGPYARLTTPLTYAENNFVTEQVQ-LHA 59
Query: 439 ELWIGLNDLKLQMNFEWSDGSLVSFTHWHPFEPNNFRDSLEDCVTIWGPEGRWNDSPCNQ 498
L + DG +T++ EP + C+TI G W PC
Sbjct: 60 NWHTWLEYVADGTYIVGDDGKPPIYTNFAVGEPFSVNV--GYCITIG-MNGYWYAQPCPD 116
Query: 499 SLPSICK 505
S +C+
Sbjct: 117 SHSVLCE 123
Score = 91.1 bits (223), Expect = 1e-19
Identities = 22/129 (17%), Positives = 42/129 (32%), Gaps = 9/129 (6%)
Query: 235 CETFWDKDQLTDSCYQFNFQSTLSWREAWASCEQQG---ADLLSITEIHEQTYINGLLTG 291
C T CY ++ A A C + A L + E ++ +
Sbjct: 1 CPTGT--KIYNAKCYAA-LGMDMTRDNALAYCRKTYGPYARLTTPLTYAENNFVTEQVQL 57
Query: 292 YSSTLWIGLNDLDTSGGWQWSDNSPLKYLNWESDQPDNPSEENCGVIRTESSGGWQNRDC 351
+++ L + D P Y N+ +P + + C I +G W + C
Sbjct: 58 HANW-HTWLEYVADGTYIVGDDGKPPIYTNFAVGEPFSVNVGYCITIGM--NGYWYAQPC 114
Query: 352 SIALPYVCK 360
+ +C+
Sbjct: 115 PDSHSVLCE 123
Score = 84.1 bits (205), Expect = 2e-17
Identities = 23/126 (18%), Positives = 40/126 (31%), Gaps = 10/126 (7%)
Query: 829 WLRFQEAEYKFFEHHSTWAQAQRICTWF---QAELTSVHSQAELDFLSHNLQKFSRAQEQ 885
+ Y T A C A LT+ + AE +F++ +Q +
Sbjct: 5 TKIYNAKCYAALGMDMTRDNALAYCRKTYGPYARLTTPLTYAENNFVTEQVQLHANW--- 61
Query: 886 HWWIGLHTSESDGRFRWTDGSIINFISWAPGKPRPVGKDKKCVYMTASREDWGDQRCLTA 945
L DG + ++A G+P V C+ + W Q C +
Sbjct: 62 --HTWLEYVADGTYIVGDDGKPPIYTNFAVGEPFSVNVG-YCITIG-MNGYWYAQPCPDS 117
Query: 946 LPYICK 951
+C+
Sbjct: 118 HSVLCE 123
Score = 81.7 bits (199), Expect = 8e-17
Identities = 27/141 (19%), Positives = 48/141 (33%), Gaps = 22/141 (15%)
Query: 972 CPSDWIQFLNKCFQVQGQEPQSRVKWSEAQFSCEQQ---EAQLVTITNPLEQAFITASLP 1028
CP+ + KC+ G + A C + A+L T E F+T +
Sbjct: 1 CPTGTKIYNAKCYAALGM----DMTRDNALAYCRKTYGPYARLTTPLTYAENNFVTEQVQ 56
Query: 1029 N-VTFDLWIGLHASQRDFQWVEQEPLMYANWAPGEPSGPSPAPSGNKPTSCAVVLHSPSA 1087
+ W+ A + +P +Y N+A GEP + + +
Sbjct: 57 LHANWHTWLEYVADGTYIVGDDGKPPIYTNFAVGEPF--------SVNVGYCITIGM--- 105
Query: 1088 HFTGRWDDRSCTEETHGFICQ 1108
G W + C + H +C+
Sbjct: 106 --NGYWYAQPCPDS-HSVLCE 123
Score = 74.3 bits (180), Expect = 1e-14
Identities = 23/128 (17%), Positives = 40/128 (30%), Gaps = 10/128 (7%)
Query: 521 CRKGWTWHSPSCYWLGEDQVTYSEARRLCTDHG---SQLVTITNRFEQAFVSSLIYNWEG 577
C G ++ CY +T A C ++L T E FV+ +
Sbjct: 1 CPTGTKIYNAKCYAALGMDMTRDNALAYCRKTYGPYARLTTPLTYAENNFVTEQVQLHAN 60
Query: 578 EYFWTALQDLNSTGSFFWLSGDEVMYTHWNRDQP-GYSRGGCVALATGSAMGLWEVKNCT 636
+ W G +YT++ +P + G C+ + G W + C
Sbjct: 61 WHTWLEYVADG--TYIVGDDGKPPIYTNFAVGEPFSVNVGYCITI---GMNGYWYAQPCP 115
Query: 637 SFRARYIC 644
+C
Sbjct: 116 DSH-SVLC 122
Score = 73.1 bits (177), Expect = 3e-14
Identities = 25/144 (17%), Positives = 40/144 (27%), Gaps = 24/144 (16%)
Query: 669 CPQGWASDTKLRYCYKVFSSERLQDKKSWVQAQGACQELG---AQLLSLASYEEEHFVAN 725
CP G CY + A C++ A+L + +Y E +FV
Sbjct: 1 CPTGTKIYN--AKCYAAL-----GMDMTRDNALAYCRKTYGPYARLTTPLTYAENNFVTE 53
Query: 726 MLNKIFGESEPEIHEQHWFWIGLNRRDPRGGQSWRWSDGVGFSYHNFDRSRHDDDDIRGC 785
+ H W+ DG Y NF ++ C
Sbjct: 54 QVQL---------HANWHTWLEYVADG----TYIVGDDGKPPIYTNFAVGEPFSVNVGYC 100
Query: 786 AVLDLASLQWVAMQCDTQLDWICK 809
+ + W A C +C+
Sbjct: 101 ITIGMNG-YWYAQPCPDSHSVLCE 123
Score = 68.1 bits (164), Expect = 1e-12
Identities = 27/137 (19%), Positives = 45/137 (32%), Gaps = 17/137 (12%)
Query: 1261 CPQGLADSAWIPFREHCYSFHMELLLGHKEARQRCQRAGGA---VLSILDEMENVFVWEH 1317
CP G + CY+ + A C++ G + + L EN FV
Sbjct: 1 CPTG-----TKIYNAKCYAALGM-DMTRDNALAYCRKTYGPYARLTTPLTYAENNFV--- 51
Query: 1318 LQSYEGQSRGAWLGMNFNPKGGTLVWQDNTAVNYSNWGPPGLGPSMLSHNSCYWIQSNSG 1377
+ + G +V D Y+N+ P ++ C I N G
Sbjct: 52 -TEQVQLHANWHTWLEYVADGTYIVGDDGKPPIYTNFAVG--EPFSVNVGYCITIGMN-G 107
Query: 1378 LWRPGACTNITMGVVCK 1394
W C + + V+C+
Sbjct: 108 YWYAQPCPD-SHSVLCE 123
Score = 55.6 bits (132), Expect = 5e-09
Identities = 19/112 (16%), Positives = 31/112 (26%), Gaps = 7/112 (6%)
Query: 1137 FRLLQKPLRWHDALLLCES---HNASLAYVPDPYTQAFLTQAARGLRTPLWIGLAGEEGS 1193
+ L + +AL C A L F+T+ + L
Sbjct: 13 YAALGMDMTRDNALAYCRKTYGPYARLTTPLTYAENNFVTEQVQLH-ANWHTWLEYVADG 71
Query: 1194 RRYSWVSEEPLNYVGWQDGEPQQPGG--CTYVDVDGAWRTTSCDTKLQGAVC 1243
+P Y + GEP C + ++G W C +C
Sbjct: 72 TYIVGDDGKPPIYTNFAVGEPFSVNVGYCITIGMNGYWYAQPCPDSH-SVLC 122
>gi|2706599 [57..185] C-type lectin-like
Length = 129
Score = 100 bits (246), Expect = 2e-22
Identities = 29/132 (21%), Positives = 55/132 (40%), Gaps = 11/132 (8%)
Query: 382 CEPSWQPFQGHCYRLQAEKRSWQESKKACLR-GGGDLVSIHSMAELEFITKQIKQEVEE- 439
C + C++L + ++ +AC+ GG L SI S E + +K + E
Sbjct: 1 CAVGFTYVNKKCWKLITGPQRRADADEACMNLGGSTLFSIRSDIENRALVDFVKDKRIEN 60
Query: 440 LWIGLNDLKLQMN---FEWSDGSLVSFTHWHPFEPNNFRDSLEDCVTIW---GPEGRWND 493
LW GL + ++ + G+ + ++ PNN DC+ G+W
Sbjct: 61 LWTGLICVGKNSFSCTWDVNSGTAAVYNNFAEGYPNN---VYGDCIHYMTTGTQAGQWAS 117
Query: 494 SPCNQSLPSICK 505
CN+++ +C+
Sbjct: 118 GSCNETMSFVCE 129
Score = 83.7 bits (204), Expect = 2e-17
Identities = 26/133 (19%), Positives = 47/133 (34%), Gaps = 11/133 (8%)
Query: 235 CETFWDKDQLTDSCYQFNFQSTLSWREAWASC-EQQGADLLSITEIHEQTYINGLLTGYS 293
C + + C++ +A +C G+ L SI E + +
Sbjct: 1 CAVGF--TYVNKKCWKL-ITGPQRRADADEACMNLGGSTLFSIRSDIENRALVDFVKDKR 57
Query: 294 ST-LWIGLNDLD---TSGGWQWSDNSPLKYLNWESDQPDNPSEENCGVIRTESS--GGWQ 347
LW GL + S W + + Y N+ P+N +C T + G W
Sbjct: 58 IENLWTGLICVGKNSFSCTWDVNSGTAAVYNNFAEGYPNNVY-GDCIHYMTTGTQAGQWA 116
Query: 348 NRDCSIALPYVCK 360
+ C+ + +VC+
Sbjct: 117 SGSCNETMSFVCE 129
Score = 82.9 bits (202), Expect = 3e-17
Identities = 22/130 (16%), Positives = 40/130 (29%), Gaps = 12/130 (9%)
Query: 829 WLRFQEAEYKFFEHHSTWAQAQRICTWFQAE-LTSVHSQAELDFLSHNLQKFSRAQEQHW 887
+ + +K A A C L S+ S E L ++
Sbjct: 5 FTYVNKKCWKLITGPQRRADADEACMNLGGSTLFSIRSDIENRALVDFVKDKRIEN---L 61
Query: 888 WIGLHTSE---SDGRFRWTDGSIINFISWAPGKPRPVGKDKKCVYMTASRED---WGDQR 941
W GL + G+ + ++A G P V D C++ + W
Sbjct: 62 WTGLICVGKNSFSCTWDVNSGTAAVYNNFAEGYPNNVYGD--CIHYMTTGTQAGQWASGS 119
Query: 942 CLTALPYICK 951
C + ++C+
Sbjct: 120 CNETMSFVCE 129
Score = 81.7 bits (199), Expect = 7e-17
Identities = 28/129 (21%), Positives = 52/129 (39%), Gaps = 6/129 (4%)
Query: 521 CRKGWTWHSPSCYWLGEDQVTYSEARRLCTD-HGSQLVTITNRFEQAFVSSLIYNWEGEY 579
C G+T+ + C+ L ++A C + GS L +I + E + + + E
Sbjct: 1 CAVGFTYVNKKCWKLITGPQRRADADEACMNLGGSTLFSIRSDIENRALVDFVKDKRIEN 60
Query: 580 FWTALQDLN---STGSFFWLSGDEVMYTHWNRDQPGYSRGGCVAL-ATGSAMGLWEVKNC 635
WT L + + ++ SG +Y ++ P G C+ TG+ G W +C
Sbjct: 61 LWTGLICVGKNSFSCTWDVNSGTAAVYNNFAEGYPNNVYGDCIHYMTTGTQAGQWASGSC 120
Query: 636 TSFRARYIC 644
++C
Sbjct: 121 NETM-SFVC 128
Score = 77.8 bits (189), Expect = 1e-15
Identities = 27/144 (18%), Positives = 52/144 (35%), Gaps = 22/144 (15%)
Query: 972 CPSDWIQFLNKCFQVQGQEPQSRVKWSEAQFSCEQQEAQ-LVTITNPLEQAFITASLPN- 1029
C + KC+++ + ++A +C L +I + +E + + +
Sbjct: 1 CAVGFTYVNKKCWKLITG----PQRRADADEACMNLGGSTLFSIRSDIENRALVDFVKDK 56
Query: 1030 VTFDLWIGLHA-----SQRDFQWVEQEPLMYANWAPGEPSGPSPAPSGNKPTSCAVVLHS 1084
+LW GL + +Y N+A G P+ N C + +
Sbjct: 57 RIENLWTGLICVGKNSFSCTWDVNSGTAAVYNNFAEGYPN--------NVYGDCIHYMTT 108
Query: 1085 PSAHFTGRWDDRSCTEETHGFICQ 1108
+ G+W SC ET F+C+
Sbjct: 109 GTQ--AGQWASGSC-NETMSFVCE 129
Score = 70.0 bits (169), Expect = 2e-13
Identities = 26/146 (17%), Positives = 49/146 (32%), Gaps = 22/146 (15%)
Query: 669 CPQGWASDTKLRYCYKVFSSERLQDKKSWVQAQGACQEL-GAQLLSLASYEEEHFVANML 727
C G+ + C+K+ + + A AC L G+ L S+ S E + + +
Sbjct: 1 CAVGFTYVN--KKCWKLIT-----GPQRRADADEACMNLGGSTLFSIRSDIENRALVDFV 53
Query: 728 NKIFGESEPEIHEQHWFWIGLNRRDPRG-GQSWRWSDGVGFSYHNFDRSRHDDDDIRGCA 786
W GL +W + G Y+NF + ++ C
Sbjct: 54 KD---------KRIENLWTGLICVGKNSFSCTWDVNSGTAAVYNNFA-EGYPNNVYGDCI 103
Query: 787 VLDLAS---LQWVAMQCDTQLDWICK 809
QW + C+ + ++C+
Sbjct: 104 HYMTTGTQAGQWASGSCNETMSFVCE 129
Score = 61.5 bits (147), Expect = 1e-10
Identities = 28/139 (20%), Positives = 48/139 (34%), Gaps = 15/139 (10%)
Query: 1261 CPQGLADSAWIPFREHCYSFHMELLLGHKEARQRCQRAGGAVL-SILDEMENVFVWEHLQ 1319
C G + + C+ +A + C GG+ L SI ++EN + + ++
Sbjct: 1 CAVG-----FTYVNKKCWKLITG-PQRRADADEACMNLGGSTLFSIRSDIENRALVDFVK 54
Query: 1320 -SYEGQSRGAWLGMNFNPKGGTLVWQDNTAVNYSNWGPPGLGPSMLSHNSCYWIQSNS-- 1376
+ + N T TA Y+N+ G + C +
Sbjct: 55 DKRIENLWTGLICVGKNSFSCTWDVNSGTAAVYNNFAE---GYPNNVYGDCIHYMTTGTQ 111
Query: 1377 -GLWRPGACTNITMGVVCK 1394
G W G+C TM VC+
Sbjct: 112 AGQWASGSCNE-TMSFVCE 129
Score = 49.4 bits (116), Expect = 4e-07
Identities = 27/117 (23%), Positives = 40/117 (34%), Gaps = 11/117 (9%)
Query: 1137 FRLLQKPLRWHDALLLCESHNAS-LAYVPDPYTQAFLTQAARGLR-TPLWIGL---AGEE 1191
++L+ P R DA C + S L + L + R LW GL
Sbjct: 13 WKLITGPQRRADADEACMNLGGSTLFSIRSDIENRALVDFVKDKRIENLWTGLICVGKNS 72
Query: 1192 GSRRYSWVSEEPLNYVGWQDGEPQ-QPGGCTYV----DVDGAWRTTSCDTKLQGAVC 1243
S + S Y + +G P G C + G W + SC+ + VC
Sbjct: 73 FSCTWDVNSGTAAVYNNFAEGYPNNVYGDCIHYMTTGTQAGQWASGSCNETM-SFVC 128
>gi|2502062 [25..167] C-type lectin-like
Length = 143
Score = 92.6 bits (227), Expect = 4e-20
Identities = 28/139 (20%), Positives = 49/139 (35%), Gaps = 19/139 (13%)
Query: 386 WQPFQGHCYRLQAEKRSWQESKKACLRGGGDLVSIHSMAELEFITKQIK--QEVEELWIG 443
Q C+ L + ++ ++ +AC R G L+++ S + I+ + WIG
Sbjct: 4 SQCVGNECFALFQDPVTFLDASQACQRLQGHLMTVRSSVAADVISLLVSDSSMDSRPWIG 63
Query: 444 L---------NDLKLQMNFEWSDGS-LVSFTHWHPFEPNNFRDSLEDCVTIWGP------ 487
L L F+W G S++ W + CVT+
Sbjct: 64 LQLPQGCGDPVHLGPLRGFQWVTGDNHTSYSRWARPNDQSPPLCGPLCVTVSTATEAAPG 123
Query: 488 EGRWNDSPCNQSL-PSICK 505
E W + PC +C+
Sbjct: 124 EPAWEEKPCENETKGFLCE 142
Score = 82.1 bits (200), Expect = 6e-17
Identities = 33/150 (22%), Positives = 59/150 (39%), Gaps = 22/150 (14%)
Query: 973 PSDWIQFLNKCFQVQGQEPQSRVKWSEAQFSCEQQEAQLVTITNPLEQAFITASLPNVT- 1031
P N+CF + V + +A +C++ + L+T+ + + I+ + + +
Sbjct: 1 PKGSQCVGNECFALFQD----PVTFLDASQACQRLQGHLMTVRSSVAADVISLLVSDSSM 56
Query: 1032 -FDLWIGLH-----------ASQRDFQWVEQEPLM-YANWAPGEPSGPSPAPSGNKPTSC 1078
WIGL R FQWV + Y+ WA +P P
Sbjct: 57 DSRPWIGLQLPQGCGDPVHLGPLRGFQWVTGDNHTSYSRWARPND----QSPPLCGPLCV 112
Query: 1079 AVVLHSPSAHFTGRWDDRSCTEETHGFICQ 1108
V + +A W+++ C ET GF+C+
Sbjct: 113 TVSTATEAAPGEPAWEEKPCENETKGFLCE 142
Score = 78.2 bits (190), Expect = 9e-16
Identities = 24/140 (17%), Positives = 43/140 (30%), Gaps = 18/140 (12%)
Query: 829 WLRFQEAEYKFFEHHSTWAQAQRICTWFQAELTSVHSQAELDFLSHNLQKFSRAQEQHW- 887
+ F+ T+ A + C Q L +V S D + L S + W
Sbjct: 4 SQCVGNECFALFQDPVTFLDASQACQRLQGHLMTVRSSVAADVI-SLLVSDSSMDSRPWI 62
Query: 888 -------WIGLHTSESDGRFRWTDGS-IINFISWAPGKPR-PVGKDKKCVYMTASRED-- 936
F+W G ++ WA + P CV ++ + E
Sbjct: 63 GLQLPQGCGDPVHLGPLRGFQWVTGDNHTSYSRWARPNDQSPPLCGPLCVTVSTATEAAP 122
Query: 937 ----WGDQRCLTAL-PYICK 951
W ++ C ++C+
Sbjct: 123 GEPAWEEKPCENETKGFLCE 142
Score = 75.8 bits (184), Expect = 4e-15
Identities = 25/138 (18%), Positives = 49/138 (35%), Gaps = 21/138 (15%)
Query: 243 QLTDSCYQFNFQSTLSWREAWASCEQQGADLLSITEIHEQTYINGLLTGY--SSTLWIGL 300
+ + C+ FQ +++ +A +C++ L+++ I+ L++ S WIGL
Sbjct: 6 CVGNECFAL-FQDPVTFLDASQACQRLQGHLMTVRSSVAADVISLLVSDSSMDSRPWIGL 64
Query: 301 ---------NDLDTSGGWQWSDNSPLK-YLNWESDQPDNP--SEENCGVIRT-----ESS 343
L G+QW Y W +P C + T
Sbjct: 65 QLPQGCGDPVHLGPLRGFQWVTGDNHTSYSRWARPNDQSPPLCGPLCVTVSTATEAAPGE 124
Query: 344 GGWQNRDCSIAL-PYVCK 360
W+ + C ++C+
Sbjct: 125 PAWEEKPCENETKGFLCE 142
Score = 72.7 bits (176), Expect = 3e-14
Identities = 32/140 (22%), Positives = 51/140 (35%), Gaps = 18/140 (12%)
Query: 523 KGWTWHSPSCYWLGEDQVTYSEARRLCTDHGSQLVTITNRFEQAFVSSLI-YNWEGEYFW 581
KG C+ L +D VT+ +A + C L+T+ + +S L+ + W
Sbjct: 2 KGSQCVGNECFALFQDPVTFLDASQACQRLQGHLMTVRSSVAADVISLLVSDSSMDSRPW 61
Query: 582 TALQD---------LNSTGSFFWLSGDEVM-YTHWNRDQP---GYSRGGCVALATG---- 624
LQ L F W++GD Y+ W R CV ++T
Sbjct: 62 IGLQLPQGCGDPVHLGPLRGFQWVTGDNHTSYSRWARPNDQSPPLCGPLCVTVSTATEAA 121
Query: 625 SAMGLWEVKNCTSFRARYIC 644
WE K C + ++C
Sbjct: 122 PGEPAWEEKPCENETKGFLC 141
Score = 58.7 bits (140), Expect = 7e-10
Identities = 21/140 (15%), Positives = 37/140 (26%), Gaps = 16/140 (11%)
Query: 1270 WIPFREHCYSFHMELLLGHKEARQRCQRAGGAVLSILDEMENVFVWEHLQSYEGQS---- 1325
C++ + + +A Q CQR G ++++ + + + S
Sbjct: 4 SQCVGNECFALFQD-PVTFLDASQACQRLQGHLMTVRSSVAADVISLLVSDSSMDSRPWI 62
Query: 1326 ----RGAWLGMNFNPKGGTLVWQDNTAV-NYSNWGPPGLGPSMLSHNSCYWI------QS 1374
W +YS W P L C +
Sbjct: 63 GLQLPQGCGDPVHLGPLRGFQWVTGDNHTSYSRWARPNDQSPPLCGPLCVTVSTATEAAP 122
Query: 1375 NSGLWRPGACTNITMGVVCK 1394
W C N T G +C+
Sbjct: 123 GEPAWEEKPCENETKGFLCE 142
Score = 58.7 bits (140), Expect = 7e-10
Identities = 29/157 (18%), Positives = 55/157 (34%), Gaps = 32/157 (20%)
Query: 670 PQGWASDTKLRYCYKVFSSERLQDKKSWVQAQGACQELGAQLLSLASYEEEHFVANMLNK 729
P+G C+ +F D +++ A ACQ L L+++ S ++ +++
Sbjct: 1 PKGSQCVG--NECFALFQ-----DPVTFLDASQACQRLQGHLMTVRSSVAADVISLLVSD 53
Query: 730 IFGESEPEIHEQHWFWIGLNRRDPRGGQS-------WRWSDGVG-FSYHNFDRSRHDDDD 781
+S WIGL G ++W G SY + R
Sbjct: 54 SSMDSR--------PWIGLQLPQGCGDPVHLGPLRGFQWVTGDNHTSYSRWARPNDQSPP 105
Query: 782 IRG--CAVLDLASL------QWVAMQCDTQLD-WICK 809
+ G C + A+ W C+ + ++C+
Sbjct: 106 LCGPLCVTVSTATEAAPGEPAWEEKPCENETKGFLCE 142
Score = 47.0 bits (110), Expect = 2e-06
Identities = 25/130 (19%), Positives = 39/130 (29%), Gaps = 23/130 (17%)
Query: 1137 FRLLQKPLRWHDALLLCESHNASLAYVPDPYTQAFLTQAARGLRTP--LWIGL------- 1187
F L Q P+ + DA C+ L V ++ WIGL
Sbjct: 12 FALFQDPVTFLDASQACQRLQGHLMTVRSSVAADVISLLVSDSSMDSRPWIGLQLPQGCG 71
Query: 1188 --AGEEGSRRYSWVSEEPL-NYVGWQDGEP----QQPGGCTYVDV-------DGAWRTTS 1233
R + WV+ + +Y W C V + AW
Sbjct: 72 DPVHLGPLRGFQWVTGDNHTSYSRWARPNDQSPPLCGPLCVTVSTATEAAPGEPAWEEKP 131
Query: 1234 CDTKLQGAVC 1243
C+ + +G +C
Sbjct: 132 CENETKGFLC 141
>gi|82035 [136..263] beta-Trefoil
Length = 128
Score = 44.4 bits (104), Expect = 1e-05
Identities = 23/94 (24%), Positives = 35/94 (36%), Gaps = 5/94 (5%)
Query: 44 FLIFSHGLQG-CLEAQGGQVRVTPACNTSLPAQRWKWVSRNRL-FNLGTMQCLGTGWPGT 101
F+ GL G CL+A G+V C + Q+W + + CL T
Sbjct: 5 FVTTIVGLYGMCLQANSGKV-WLEDCTSEKAEQQWALYADGSIRPQQNRDNCLTTDANIK 63
Query: 102 NTTASLGMYECDREALNLRWHCRTLGDQLSLLLG 135
T + + C + RW + G L+L G
Sbjct: 64 GTV--VKILSCGPVSSGQRWMFKNDGTILNLYNG 95
>gi|1655439 [28..139] C-type lectin-like
Length = 112
Score = 37.3 bits (85), Expect = 0.002
Identities = 34/111 (30%), Positives = 53/111 (47%), Gaps = 6/111 (5%)
Query: 399 EKRSWQESKKACLRGGGDLVS--IHSMAELEFITKQIKQEVEELWIGLNDL-KLQMNFEW 455
E ++ ++ C G LVS + ++ I K W+G ++L K NF W
Sbjct: 3 ETMNYADAGAYCQSRGMVLVSSAMRDSTMVKAILDFTKDRRYNYWVGADNLEKGAYNFLW 62
Query: 456 SDG-SLVSFTH-WHPFEPNNFRDSLEDCVTIWGPEGRWNDSPCNQSLPSIC 504
+DG SL + + W P EP+N + S E CV IW ++D+ C + IC
Sbjct: 63 NDGVSLPTDSDLWSPDEPSNPQSS-ELCVQIWNRYNLFDDTGCGNAKRVIC 112
Score = 30.3 bits (67), Expect = 0.21
Identities = 23/87 (26%), Positives = 43/87 (48%), Gaps = 8/87 (9%)
Query: 256 TLSWREAWASCEQQGADLLSITEIHEQTYINGLLTGYSS---TLWIGLNDLDTSG-GWQW 311
T+++ +A A C+ +G L+S + + + T + +L W+G ++L+ + W
Sbjct: 4 TMNYADAGAYCQSRGMVLVS-SAMRDSTMVKAILDFTKDRRYNYWVGADNLEKGAYNFLW 62
Query: 312 SD--NSPLKYLNWESDQPDNP-SEENC 335
+D + P W D+P NP S E C
Sbjct: 63 NDGVSLPTDSDLWSPDEPSNPQSSELC 89
Score = 28.3 bits (62), Expect = 0.93
Identities = 19/75 (25%), Positives = 31/75 (41%), Gaps = 3/75 (4%)
Query: 879 FSRAQEQHWWIGLHTSESDG-RFRWTDGSIINFIS--WAPGKPRPVGKDKKCVYMTASRE 935
F++ + ++W+G E F W DG + S W+P +P + CV +
Sbjct: 38 FTKDRRYNYWVGADNLEKGAYNFLWNDGVSLPTDSDLWSPDEPSNPQSSELCVQIWNRYN 97
Query: 936 DWGDQRCLTALPYIC 950
+ D C A IC
Sbjct: 98 LFDDTGCGNAKRVIC 112
>gi|1685117 [55..161] C-type lectin-like
Length = 107
Score = 37.2 bits (85), Expect = 0.002
Identities = 27/103 (26%), Positives = 43/103 (41%), Gaps = 7/103 (6%)
Query: 402 SWQESKKACLRGGGDLVSIHSMAELEFITKQIKQEVEEL-----WIGLNDLK--LQMNFE 454
S+ +++ C + GG LV A +I ++++ EL WIG ++
Sbjct: 5 SFDKAQAICKQTGGHLVHDFRGATSSYILSELERRKSELKPQLVWIGAQKEPGITSRTWK 64
Query: 455 WSDGSLVSFTHWHPFEPNNFRDSLEDCVTIWGPEGRWNDSPCN 497
W +G +V W +PNN+ V G WND CN
Sbjct: 65 WVNGDVVQKPTWGKDQPNNYNGEQNCVVLDGGRNWLWNDVGCN 107
Score = 34.5 bits (78), Expect = 0.013
Identities = 30/103 (29%), Positives = 45/103 (43%), Gaps = 8/103 (7%)
Query: 258 SWREAWASCEQQGADLLSITEIHEQTYINGLLTGYSSTL-----WIGLNDLD--TSGGWQ 310
S+ +A A C+Q G L+ +YI L S L WIG TS W+
Sbjct: 5 SFDKAQAICKQTGGHLVHDFRGATSSYILSELERRKSELKPQLVWIGAQKEPGITSRTWK 64
Query: 311 WSDNSPLKYLNWESDQPDN-PSEENCGVIRTESSGGWQNRDCS 352
W + ++ W DQP+N E+NC V+ + W + C+
Sbjct: 65 WVNGDVVQKPTWGKDQPNNYNGEQNCVVLDGGRNWLWNDVGCN 107
Score = 28.7 bits (63), Expect = 0.74
Identities = 22/99 (22%), Positives = 39/99 (39%), Gaps = 4/99 (4%)
Query: 848 QAQRICTWFQAELTSVHSQAELDFLSHNLQ-KFSRAQEQHWWIGLHTSE--SDGRFRWTD 904
+AQ IC L A ++ L+ + S + Q WIG + ++W +
Sbjct: 8 KAQAICKQTGGHLVHDFRGATSSYILSELERRKSELKPQLVWIGAQKEPGITSRTWKWVN 67
Query: 905 GSIINFISWAPGKPRPVGKDKKCVYMTASRE-DWGDQRC 942
G ++ +W +P ++ CV + R W D C
Sbjct: 68 GDVVQKPTWGKDQPNNYNGEQNCVVLDGGRNWLWNDVGC 106
Score = 27.5 bits (60), Expect = 1.8
Identities = 26/103 (25%), Positives = 40/103 (38%), Gaps = 9/103 (8%)
Query: 541 TYSEARRLCTDHGSQLVTITNRFEQAFVSSLIYNWEGE----YFWTALQDLNSTGS--FF 594
++ +A+ +C G LV +++ S + + E W Q S +
Sbjct: 5 SFDKAQAICKQTGGHLVHDFRGATSSYILSELERRKSELKPQLVWIGAQKEPGITSRTWK 64
Query: 595 WLSGDEVMYTHWNRDQPGYSRG--GCVALATGSAMGLWEVKNC 635
W++GD V W +DQP G CV L G LW C
Sbjct: 65 WVNGDVVQKPTWGKDQPNNYNGEQNCVVL-DGGRNWLWNDVGC 106
Score = 27.1 bits (59), Expect = 1.9
Identities = 15/70 (21%), Positives = 33/70 (46%), Gaps = 5/70 (7%)
Query: 695 KSWVQAQGACQELGAQLLSLASYEEEHFVANMLNKIFGESEPEIHEQHWFWIGLNRRDPR 754
+S+ +AQ C++ G L+ ++ + L + E +P++ WIG +
Sbjct: 4 ESFDKAQAICKQTGGHLVHDFRGATSSYILSELERRKSELKPQL-----VWIGAQKEPGI 58
Query: 755 GGQSWRWSDG 764
++W+W +G
Sbjct: 59 TSRTWKWVNG 68
>gi|438998 [1..297] S-adenosyl-L-methionine-dependent methyltransferases
Length = 297
Score = 28.7 bits (63), Expect = 0.73
Identities = 13/41 (31%), Positives = 19/41 (45%), Gaps = 2/41 (4%)
Query: 1125 APGTELSYLNGTFRLLQKPLRWHDALLLCESHNASLAYVPD 1165
APGT + YL F L ++W L+ H+ L + D
Sbjct: 70 APGTHIRYLRDHFYNLGVIIKW--MLIDGRHHDPILNGLRD 108
>gi|2507361 [53..298] Periplasmic binding protein-like I
Length = 246
Score = 28.4 bits (63), Expect = 0.87
Identities = 11/72 (15%), Positives = 22/72 (30%), Gaps = 6/72 (8%)
Query: 266 CEQQGADLLSITEIH------EQTYINGLLTGYSSTLWIGLNDLDTSGGWQWSDNSPLKY 319
+ G DL + +Q I+ + + +G + + + P+
Sbjct: 18 ARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSFPDLQKQVASLPVIE 77
Query: 320 LNWESDQPDNPS 331
L D P S
Sbjct: 78 LVNAIDAPQVKS 89
>gi|115715 [1..97] Ferredoxin-like
Length = 97
Score = 28.0 bits (62), Expect = 1.2
Identities = 16/84 (19%), Positives = 26/84 (30%), Gaps = 3/84 (3%)
Query: 815 DVREPDDSPQGRREWLRFQEAEY-KFFEHHSTWAQAQRICT--WFQAELTSVHSQAELDF 871
V+ P D + L+ E E + + TW RI + + V S +
Sbjct: 8 TVKLPVDMDPAKAARLKADEKELAQRLQREGTWRHLWRIAVGHYANYSVFDVSSVEACND 67
Query: 872 LSHNLQKFSRAQEQHWWIGLHTSE 895
L F + + H S
Sbjct: 68 TLMQLPLFPYMDIEVDGLCRHPSS 91
>gi|2500689 [29..252] Periplasmic binding protein-like I
Length = 224
Score = 27.3 bits (60), Expect = 1.8
Identities = 9/72 (12%), Positives = 21/72 (28%), Gaps = 7/72 (9%)
Query: 266 CEQQGADLLSITEIH----EQTYINGLLTGYSSTLWIGLNDLDTSGG---WQWSDNSPLK 318
++ G D + T + + +T + IG ++ + S N P+
Sbjct: 27 AQELGMDYVEFTSENSPAKQMDNARTAVTKGVDAIVIGPVSSTSTPPLLAYLKSQNIPIA 86
Query: 319 YLNWESDQPDNP 330
+
Sbjct: 87 FAGIGPQPGQTD 98
>gi|1536958 [63..316] Periplasmic binding protein-like I
Length = 254
Score = 27.3 bits (60), Expect = 2.0
Identities = 7/71 (9%), Positives = 17/71 (23%), Gaps = 5/71 (7%)
Query: 266 CEQQGADLLSITEIH----EQTYINGLLTGYSSTLWIGLNDLDTSGGWQWSDNS-PLKYL 320
Q G ++ + E YI +L I + + +
Sbjct: 27 ASQNGYQVMIGNSNYSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFF 86
Query: 321 NWESDQPDNPS 331
+ + +
Sbjct: 87 DSQLYEHRTSW 97
>gi|2650282 [2..154] P-loop containing nucleotide triphosphate hydrolases
Length = 153
Score = 27.1 bits (59), Expect = 2.1
Identities = 17/123 (13%), Positives = 30/123 (23%), Gaps = 8/123 (6%)
Query: 1355 GPPGLGPSMLSHNSCYWIQSNSGLWR---PGACTNITMGVVCKLPRAEQSSFSPSALPEN 1411
G G G + L + Y N +G + + +
Sbjct: 26 GQYGTGKTTLGLHFIYEGLKNGEACMIISFDEDEESIIGDAKSVGMDLTAFGDKVHIVRL 85
Query: 1412 PAALVVVLMAVLLLLALLTAA-----LILYRRRQSIERGAFEGARYSRSSSSPTEATEKN 1466
A+ V + L +L +E + RYS ++ E
Sbjct: 86 EASEVKKSLEKLESDLPEIVRSLGVSRMLVDSISVLETLFDDAGRYSMLAAFRKMLKENG 145
Query: 1467 ILV 1469
I
Sbjct: 146 ITA 148
>gi|1708629 [67..339] Periplasmic binding protein-like I
Length = 273
Score = 26.9 bits (59), Expect = 2.5
Identities = 7/71 (9%), Positives = 22/71 (30%), Gaps = 5/71 (7%)
Query: 266 CEQQGADLLSITEIH----EQTYINGLLTGYSSTLWIGLNDLDTSGGWQWSDNS-PLKYL 320
C+Q G ++ + E+ + L L + + +++ P +
Sbjct: 27 CDQYGYSIMVCNTDNSPEKEREMLLKLEAHSVEGLILNATGENKDVLRAFAEQQIPTILI 86
Query: 321 NWESDQPDNPS 331
+ + +
Sbjct: 87 DRKLPDLKLDT 97
>gi|797337 [17..308] Periplasmic binding protein-like I
Length = 292
Score = 26.1 bits (57), Expect = 3.7
Identities = 10/73 (13%), Positives = 21/73 (28%), Gaps = 8/73 (10%)
Query: 266 CEQQGADLLSITEIH----EQTYINGLLTGYSSTLWIG---LNDLDTSGGWQWSDNSPLK 318
G ++ T E+ + LL L I +++D + P+
Sbjct: 27 ASAHGFMVILATSDEDIALERDAVRVLLAKRVDGLIISPTSSSEVDHLISAS-ERDCPVV 85
Query: 319 YLNWESDQPDNPS 331
L+ +
Sbjct: 86 LLDRRVPVLRADT 98
>gi|729214 [63..305] Periplasmic binding protein-like I
Length = 243
Score = 25.7 bits (56), Expect = 5.4
Identities = 11/69 (15%), Positives = 17/69 (23%), Gaps = 3/69 (4%)
Query: 266 CEQQGADLLSITEIHEQT---YINGLLTGYSSTLWIGLNDLDTSGGWQWSDNSPLKYLNW 322
+ G + I E LL + L + + N
Sbjct: 26 ASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANC 85
Query: 323 ESDQPDNPS 331
+D P PS
Sbjct: 86 VADDPALPS 94
>gi|2244909 [587..959] beta/alpha (TIM)-barrel
Length = 373
Score = 25.7 bits (56), Expect = 5.6
Identities = 16/62 (25%), Positives = 23/62 (36%), Gaps = 12/62 (19%)
Query: 1158 ASLAYVPDPYTQAFL----------TQAARGLRTPLWIGLAGEEGSRRYS--WVSEEPLN 1205
A DP+ T+ R R L +G+ GE G S + +E L+
Sbjct: 293 AKGILQHDPFEVLDQQGVGQLIKMATEKGRAARPSLKVGICGEHGGDPSSVGFFAEAGLD 352
Query: 1206 YV 1207
YV
Sbjct: 353 YV 354
>gi|137196 [30..266] P-loop containing nucleotide triphosphate hydrolases
Length = 237
Score = 25.5 bits (54), Expect = 7.2
Identities = 3/23 (13%), Positives = 3/23 (13%)
Query: 1355 GPPGLGPSMLSHNSCYWIQSNSG 1377
GP S
Sbjct: 31 GPSKSFKSNFGLTMVSSYMRQYP 53
>gi|586847 [59..303] Periplasmic binding protein-like I
Length = 245
Score = 25.3 bits (55), Expect = 7.5
Identities = 9/70 (12%), Positives = 16/70 (22%), Gaps = 5/70 (7%)
Query: 266 CEQQGADLLSITEIH----EQTYINGLLTGYSSTLWIGLNDLDTSGGWQWSDNSPLKYLN 321
Q + + E Y+ L T L I + +
Sbjct: 27 AFQHEYATTLLPTNYNPDIEIKYLELLRTKKIDGLIITSRANHWDSILAYQEY-GPVIAC 85
Query: 322 WESDQPDNPS 331
++ D P
Sbjct: 86 EDTGDIDVPC 95
>gi|628933 [59..301] Periplasmic binding protein-like I
Length = 243
Score = 25.3 bits (55), Expect = 7.6
Identities = 7/71 (9%), Positives = 16/71 (21%), Gaps = 5/71 (7%)
Query: 266 CEQQGADLLSITEIH----EQTYINGLLTGYSSTLWIGLNDLDTSGGWQWSDNS-PLKYL 320
C+ G +S T+ E + + + ++ P +
Sbjct: 32 CKNHGYATISTTKGQSIEIELEVQEMIHYHSVDGFIVLYSKKSDPIIDILKSHAMPYVII 91
Query: 321 NWESDQPDNPS 331
D
Sbjct: 92 GKPLTDDDIIH 102
>gi|2500694 [73..586] Periplasmic binding protein-like II
Length = 514
Score = 25.3 bits (55), Expect = 8.3
Identities = 11/87 (12%), Positives = 22/87 (24%), Gaps = 21/87 (24%)
Query: 264 ASCEQQGADLLSITEIHEQTYINGLLTGYSSTLWIGLNDLDTS-GGWQWSDNSPLKYLN- 321
+ ++ G + + + Y N D D + W S + + +
Sbjct: 400 QNLKRIGIE-ARVRTVDPSQYTNRKRAF----------DYDVTWEVWGQSLSPGNEQADY 448
Query: 322 WESDQPDNPSEENCGVIRTESSGGWQN 348
W S N G +
Sbjct: 449 WGSAAATRQGSRN--------YAGISD 467
>gi|121769 [3..207] Class I glutamine amidotransferases
Length = 205
Score = 25.0 bits (54), Expect = 8.5
Identities = 9/49 (18%), Positives = 17/49 (34%), Gaps = 5/49 (10%)
Query: 702 GACQELGAQLLSLASYEEEHFVANMLNKIFGESEPEIHEQHWFWIGLNR 750
G C G Q +++ V + FG ++ E+ G+
Sbjct: 82 GVC--YGMQTMAMQLGGH---VEASNEREFGYAQVEVVNDSALVRGIED 125
>gi|1616941 [10..247] Metzincin-like
Length = 238
Score = 24.9 bits (54), Expect = 9.0
Identities = 3/19 (15%), Positives = 5/19 (25%)
Query: 176 YTIQGNSHGKPCTIPFKYD 194
+ G + P D
Sbjct: 219 QNTGQDFKGAYSSAPLLDD 237
>gi|1762781 [797..951] C2 domain-like
Length = 155
Score = 24.9 bits (54), Expect = 9.0
Identities = 6/27 (22%), Positives = 10/27 (36%), Gaps = 1/27 (3%)
Query: 769 YHNFDR-SRHDDDDIRGCAVLDLASLQ 794
+ D S +D L+SL+
Sbjct: 102 RCSSDGHSYNDKTLPLATYTAKLSSLK 128
>gi|2506563 [66..342] Periplasmic binding protein-like I
Length = 277
Score = 24.9 bits (54), Expect = 9.5
Identities = 8/72 (11%), Positives = 19/72 (26%), Gaps = 6/72 (8%)
Query: 266 CEQQGADLLSITEIH----EQTYINGLLTGYSSTLWIGLNDLDTSGGWQWSDNSPLKY-- 319
E QG + + + LL + I + + ++ +
Sbjct: 25 LEAQGRMVFLLHGGKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIF 84
Query: 320 LNWESDQPDNPS 331
+ S D +
Sbjct: 85 ASRASYLDDVDT 96
Underlying Matrix: BLOSUM62
Number of sequences tested against query: 1187
Number of sequences better than 10.0: 27
Number of calls to ALIGN: 75
Length of query: 1479
Total length of test sequences: 256703
Effective length of test sequences: 209547.0
Effective search space size: 301674868.1
Initial X dropoff for ALIGN: 25.0 bits