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        Table of Previously Characterized and Novel Tumor Endothelial Markers (TEMs)

            The columns 1-7 have been derived from the SAGEnet Angiogenesis project. The entries referred to as "Description" are generated as part of the TEM @ IMP bioinformatics project.

            46 tags with the highest tumor EC (T-ECs) to normal EC (N-ECs) tag ratios are listed in descending order. Please click on the TEM number for detailed information on the sequence analysis. TEM tags underlaid in grey could not be linked with a reliable protein product.

           Please note the difference in TEM numbering compared to Croix et al, who considered only a subset of nine TEMs in their paper. The chosen TEM enumeration is corresponding to the numbering found in the table.

         
        N-ECs...number of SAGE tags found in normal EC SAGE libraries (contains 96,694 tags).
        T-ECs...number of SAGE tags found in tumor EC SAGE libraries (contains 96,588 tags).
        HUVEC...number of SAGE tags found in HUVEC SAGE libraries (contains 290,000 tags).
        HMVEC...number of SAGE tags found in HMVEC SAGE libraries (contains 111,000 tags).
        Cell Lines   ...number of SAGE tags found in non-endothelial cell line SAGE libraries (contains 1.8 million tags).
         ...Tag numbers for each group have been normalized to 100,000 transcripts.


        no. Tag Sequence N-ECs T-ECs HUVEC HMVEC Cell Lines Description
        1 GGGGCTGCCCA 0 28 0 2 0 Croix et al: TEM1: Receptor, internalization & potential ECM interacting
        2 GATCTCCGTGT 0 25 0 0 0 Croix et al: TEM2: Rap subfamily member
        3 CATTTTTATCT 0 23 0 0 0 Croix et al: TEM3, TEM7: Shares homology with two protein-families which have been implicated in axon migration. Sequence not confirmed, middle seq. part containing extracellular domains (PSI, NIDO) shared in homologues (Dros und C.el.) Potential type I TM protein
        4 CTTTCTTTGAG 0 22 6 20 1 Dkk-3: thyroid binding colipase fold
        5 TATTAACTCTC 0 21 1 3 1 Croix et al: TEM4: DH family Rho GEF potentially linking to MAPK patways
        6 CAGGAGACCCC 0 16 2 0 0 MMP-11 Group II MMP, with a wide range of substrate specificity: weak activity against non-fibrillar collagen, LM, FN
        7 GGAAATGTCAA 1 31 53 22 1 MMP-2 = type IV collagenase (Group III MMP): Gelatin, types IV, V and I collagen, LM, FN, proMMP-9,13
        8 CCTGGTTCAGT 0 15 0 0 0 HES family bHLH TF
        9 TTTTTAAGAAC 0 14 1 4 0 Croix et al: TEM5: Secretin-like receptor with potential SH3 binding sites
        10 TTTGGTTTTCC 5 139 0 16 0 COL1A2 major structural component of the ECM
        11 ATTTTGTATGA 0 13 4 8 0 nidogen, was originally thought to stabilize the basement membrane by connnecting the laminin and collagen IV networks; recent studies show that nidogen 1 is not required for basement membrane formation or maintenance neither in mice nor in C.elegans.
        12 ACTTTAGATGG 1 23 0 15 0 COL6A3 has a critical role in colVI assembly, colVI has both cell adhesion properties and a diverse range of ECM protein binding activities.
        13 GAGTGAGACCC 3 63 0 0 1 Thy-1 is a marker of adult but not embryonic angiogenesis, the function of Thy-1 is unknown.
        14 GTACACACACC 0 10 0 0 0 cystatin family member, cystein protease inhibitors
        15 CCACAGGGGAT 2 38 0 2 1 col3A1 component of the basement membrane
        16 TTAAAAGTCAC 1 19 1 3 1 Croix et al: TEM6: tensin-like, partial sequence
        17 ACAGACTGTTA 4 74 0 0 0 Croix et al: TEM7: Shares homology with two protein-families which have been implicated in axon migration. Sequence not confirmed, middle seq. part containing extracellular domains (PSI, NIDO) shared in homologues (Dros und C.el.) Potential type I TM protein
        18 CCACTGCAACC 1 18 0 1 0 protein sequence unknown
        19 CTATAGGAGAC 1 18 1 1 0 Croix et al: TEM8: hypothetical, partial sequence hinting on a transmembrane or extracellular protein with relation to adhesion
        20 GTTCCACAGAA 0 9 0 3 0 COL1A2 major structural component of the ECM
        21_1 TACCACCTCCC 0 9 4 1 1 pregnancy specific beta-1-glycoprotein 1
        21_2 TACCACCTCCC 0 9 4 1 1 formin family member
        22 GCCCTTTCTCT 1 17 3 1 2 Croix et al: TEM9: uPARAP, endocytic receptor interacting with uPAR and collagenV, potential role in ECM remodeling
        23 TTAAATAGCAC 2 33 0 4 0 COL1A1 (Tem23, 38), two alpha 1(I) and one alpha 2(I) chains (Tem10,20,40) form a collagen I fiber
        24 AGACATACTGA 1 16 1 0 0 human tensin, links cytoskeleton, plasma membrane and signaltransduction
        25 TCCCCCAGGAG 1 16 0 0 0 BMP-1 is involved in the processing of several procollagens to collagens
        26 AGCCCAAAGTG 0 8 0 0 0 Est sequence unknown
        27 ACTACCATAAC 0 8 0 0 0 hSlit-3 (MEGF5) provide a matrix for the migration axons, link multiple ligands and thereby mediate cell interactions
        28 TACAAATCGTT 0 8 0 0 0 nedrin related Rho-GAP with a potential role in the formation of macromolecular complexes
        29 TTGGGTGAAAA 0 8 0 0 0 protein sequence unknown
        30 CATTATCCAAA 0 8 0 0 0 Integrin alpha1, binds collagens I, collagen IV, laminin, regulates collagen synthesis, tumor vascularization was altered in the alpha1-null mice
        31_1 AGAAACCACGG 0 8 2 7 0 collagen, type IV, alpha 1
        31_2 AGAAACCACGG 0 8 2 7 0 KIAA1164
        32 ACCAAAACCAC 0 8 0 3 0 Est sequence unknown
        33 TGAAATAAAC 0 8 3 1 1 Est sequence unknown
        34 TTTGGTTTCC 1 15 0 0 0 protein sequence unknown
        35 GTGGAGACGGA 1 15 1 2 1 connexin 45, involved in vascular development
        36 TTTGTGTTGTA 1 14 2 0 0 COL12A1, three a1(XII) chains compose Type XII collagens, non-fibrillar collagens termed FACIT(fibril-associated) provide specific molecular bridges between fibrils and other matrix components
        37 TTATGTTTAAT 3 39 0 0 1 lumican, acts as a tissue organizers, orienting and ordering collagen fibrils
        38 TGGAAATGACC 15 179 0 40 0 COL1A1 (Tem23, 38), Tem23 and Tem38 are potentially derived from the same transcript, with only Tem38 being obtained by classical tag derivation.
        39 TGCCACACAGT 1 13 0 2 0 TGFbeta 3, potentially involved in ECM production
        40 GATGAGGAGAC 3 35 0 18 1 COL2A1, the tag is localized in the proposed CDS and thus potentially results in a transcript differing from tem20, tem40
        41 ATCAAAGGTTT 2 23 0 0 0 olfactomedin-like, OLF domain is localized C-terminally in resemblance to secreted olfactomedin-related glycoproteins
        42 AGTCACATAGT 1 11 2 0 0 RGS5 border-line reliability as the tag is unreliable: still considered due to the clear expression preference in aorta PolyA
        43 TTCGGTTGGTC 4 45 0 19 0 no Unigen cluster matching the tag
        44 CCCCACACGGG 2 21 0 0 0 unknown protein
        45 GGCTTGCCTTT 1 10 0 10 1 Tropomyosin1 (alpha)
        46 ATCCCTTCCCG 1 10 1 0 0 peanut-like protein 1


        Author: Maria Novatchkova (novatchkova@imp.ac.at)
        Last modified: May 2001