RID=1056971020-012121-3147, At1g15790 [1-145] BLASTP 2.2.6 [Apr-09-2003]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

RID: 1056971020-012121-3147

Query= At1g15790 [1-145] (145 letters)

Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 1,459,938 sequences; 468,778,868 total letters

If you have any problems or questions with the results of this search
please refer to the BLAST FAQs




Results of PSI-Blast iteration 1

Distribution of 14 Blast Hits on the Query Sequence



Legend:

New sequence mark - means that the alignment score was below the threshold on the previous iteration

Checked mark - means that the alignment was checked on the previous iteration


Hit list size
Sequences with E-value BETTER than threshold
Score E Sequences producing significant alignments: (bits) Value

New sequence mark gi|22329593|ref|NP_173031.2| expressed protein [Arabidopsis thal... 269 5e-72
New sequence mark gi|15218294|ref|NP_173030.1| expressed protein [Arabidopsis thal... 117 2e-26
New sequence mark gi|25511635|pir||B86292 F7H2.12 protein - Arabidopsis thaliana >... 102 9e-22
New sequence mark gi|24461860|gb|AAN62347.1|AF506028_14 CTV.20 [Poncirus trifoliata] 100 5e-21
New sequence mark gi|28071313|dbj|BAC56002.1| P0705A05.20 [Oryza sativa (japonica ... 95 2e-19
New sequence mark gi|24461867|gb|AAN62354.1|AF506028_23 CTV.22 [Poncirus trifoliata] 93 8e-19

Sequences with E-value WORSE than threshold

  gi|27260984|dbj|BAC45101.1| OJ1705_C03.23 [Oryza sativa (japonic... 37 0.049
  gi|7493813|pir||T18235 transcription activator GAL11 homolog - y... 33 0.60

Alignments
>gi|22329593|ref|NP_173031.2|   expressed protein [Arabidopsis thaliana]
 gi|25513497|pir||C86292   F7H2.13 protein - Arabidopsis thaliana
 gi|8927658|gb|AAF82149.1|AC034256_13   ESTs gb|AI995735, gb|T44391, gb|AA395434 come from this gene.
           [Arabidopsis thaliana]
 gi|12083256|gb|AAG48787.1|AF332424_1   unknown protein [Arabidopsis thaliana]
          Length = 179

 Score =  269 bits (688), Expect = 5e-72
 Identities = 145/145 (100%), Positives = 145/145 (100%)

Query: 1   MDTTGDWRTQFPSASRSRIVNKIMETQLKQLPFIRPEGTNELRKIAVRFEEKLFNNASNQ 60
           MDTTGDWRTQFPSASRSRIVNKIMETQLKQLPFIRPEGTNELRKIAVRFEEKLFNNASNQ
Sbjct: 1   MDTTGDWRTQFPSASRSRIVNKIMETQLKQLPFIRPEGTNELRKIAVRFEEKLFNNASNQ 60

Query: 61  TEYLRQICMKMLNMETKSQNAAGSSSADDNTPPLVPEPSVPNNEPAVNTGDWRTQQPQDS 120
           TEYLRQICMKMLNMETKSQNAAGSSSADDNTPPLVPEPSVPNNEPAVNTGDWRTQQPQDS
Sbjct: 61  TEYLRQICMKMLNMETKSQNAAGSSSADDNTPPLVPEPSVPNNEPAVNTGDWRTQQPQDS 120

Query: 121 RQKNINALLDTLKKIVPHSGKEGID 145
           RQKNINALLDTLKKIVPHSGKEGID
Sbjct: 121 RQKNINALLDTLKKIVPHSGKEGID 145

 Score = 62.4 bits (150), Expect = 1e-09
 Identities = 34/71 (47%), Positives = 47/71 (66%)

Query: 4   TGDWRTQFPSASRSRIVNKIMETQLKQLPFIRPEGTNELRKIAVRFEEKLFNNASNQTEY 63
           TGDWRTQ P  SR + +N +++T  K +P    EG +EL +IAV  EE +FN+A NQ +Y
Sbjct: 109 TGDWRTQQPQDSRQKNINALLDTLKKIVPHSGKEGIDELMRIAVSLEELIFNSAINQEDY 168

Query: 64  LRQICMKMLNM 74
           L +I +KM  M
Sbjct: 169 LGKISLKMRTM 179
>gi|15218294|ref|NP_173030.1|   expressed protein [Arabidopsis thaliana]
          Length = 1335

 Score =  117 bits (294), Expect = 2e-26
 Identities = 69/115 (60%), Positives = 79/115 (68%)

Query: 4   TGDWRTQFPSASRSRIVNKIMETQLKQLPFIRPEGTNELRKIAVRFEEKLFNNASNQTEY 63
           TGDWRTQ P  SR +IVNKIMET  K LPF  PEG NELR+IA RFEEK+F+ A NQT+Y
Sbjct: 19  TGDWRTQLPPDSRQKIVNKIMETLKKHLPFSGPEGINELRRIAARFEEKIFSGALNQTDY 78

Query: 64  LRQICMKMLNMETKSQNAAGSSSADDNTPPLVPEPSVPNNEPAVNTGDWRTQQPQ 118
           LR+I MKML METKSQNAAGSS+A           S+P N+  +  G   T Q Q
Sbjct: 79  LRKISMKMLTMETKSQNAAGSSAAIPAANNGTSIDSIPTNQGQLLPGSLSTNQSQ 133

 Score = 64.3 bits (155), Expect = 3e-10
 Identities = 31/48 (64%), Positives = 40/48 (83%)

Query: 98  PSVPNNEPAVNTGDWRTQQPQDSRQKNINALLDTLKKIVPHSGKEGID 145
           PS+PN EPA++TGDWRTQ P DSRQK +N +++TLKK +P SG EGI+
Sbjct: 8   PSLPNGEPAMDTGDWRTQLPPDSRQKIVNKIMETLKKHLPFSGPEGIN 55
>gi|25511635|pir||B86292   F7H2.12 protein - Arabidopsis thaliana
 gi|8927657|gb|AAF82148.1|AC034256_12   EST gb|N38213 comes from this gene. [Arabidopsis thaliana]
          Length = 1366

 Score =  102 bits (254), Expect = 9e-22
 Identities = 69/146 (47%), Positives = 79/146 (54%), Gaps = 31/146 (21%)

Query: 4   TGDWRTQFPSASRSRIVNKIMETQLKQLPFIRPEGTNELRKIAVRFEEKLFNNASNQ--- 60
           TGDWRTQ P  SR +IVNKIMET  K LPF  PEG NELR+IA RFEEK+F+ A NQ   
Sbjct: 19  TGDWRTQLPPDSRQKIVNKIMETLKKHLPFSGPEGINELRRIAARFEEKIFSGALNQRFV 78

Query: 61  ----------------------------TEYLRQICMKMLNMETKSQNAAGSSSADDNTP 92
                                       T+YLR+I MKML METKSQNAAGSS+A     
Sbjct: 79  RQWTPQHGKELTFGICKAKPQYVGYEIHTDYLRKISMKMLTMETKSQNAAGSSAAIPAAN 138

Query: 93  PLVPEPSVPNNEPAVNTGDWRTQQPQ 118
                 S+P N+  +  G   T Q Q
Sbjct: 139 NGTSIDSIPTNQGQLLPGSLSTNQSQ 164

 Score = 64.7 bits (156), Expect = 2e-10
 Identities = 31/48 (64%), Positives = 40/48 (83%)

Query: 98  PSVPNNEPAVNTGDWRTQQPQDSRQKNINALLDTLKKIVPHSGKEGID 145
           PS+PN EPA++TGDWRTQ P DSRQK +N +++TLKK +P SG EGI+
Sbjct: 8   PSLPNGEPAMDTGDWRTQLPPDSRQKIVNKIMETLKKHLPFSGPEGIN 55
>gi|24461860|gb|AAN62347.1|AF506028_14   CTV.20 [Poncirus trifoliata]
          Length = 3148

 Score =  100 bits (248), Expect = 5e-21
 Identities = 50/88 (56%), Positives = 66/88 (75%), Gaps = 5/88 (5%)

Query: 6  DWRTQFPSASRSRIVNKIMETQLKQLPFIRPEGTNELRKIAVRFEEKLFNNASNQTEYLR 65
          DWR      SR RIVNKIM+T  + LPF  PEG NEL++IA RFEEK+F +A++Q++YLR
Sbjct: 16 DWRNPLSHDSRQRIVNKIMDTLKRHLPFSGPEGLNELKRIADRFEEKIFTSATSQSDYLR 75

Query: 66 QICMKMLNMETKSQNAAGSSSADDNTPP 93
          +I +KML+ME++SQNA+GS     N PP
Sbjct: 76 KISLKMLSMESRSQNASGS-----NEPP 98

 Score = 41.2 bits (95), Expect = 0.003
 Identities = 17/39 (43%), Positives = 27/39 (69%)

Query: 107 VNTGDWRTQQPQDSRQKNINALLDTLKKIVPHSGKEGID 145
           ++  DWR     DSRQ+ +N ++DTLK+ +P SG EG++
Sbjct: 12  LDPSDWRNPLSHDSRQRIVNKIMDTLKRHLPFSGPEGLN 50
>gi|28071313|dbj|BAC56002.1|   P0705A05.20 [Oryza sativa (japonica cultivar-group)]
          Length = 1359

 Score = 95.1 bits (235), Expect = 2e-19
 Identities = 50/89 (56%), Positives = 67/89 (75%)

Query: 5   GDWRTQFPSASRSRIVNKIMETQLKQLPFIRPEGTNELRKIAVRFEEKLFNNASNQTEYL 64
           GDWR+Q  S +R+RIVNKIM+T  K LP   PEG NEL+KIAVRFEEK++  A++Q++YL
Sbjct: 35  GDWRSQLQSEARNRIVNKIMDTLKKHLPVSVPEGLNELQKIAVRFEEKIYTAATSQSDYL 94

Query: 65  RQICMKMLNMETKSQNAAGSSSADDNTPP 93
           R+I +KML+METK+Q   G++    N  P
Sbjct: 95  RKISLKMLSMETKTQQNPGNAQVIQNQNP 123

 Score = 40.0 bits (92), Expect = 0.006
 Identities = 24/70 (34%), Positives = 37/70 (52%), Gaps = 11/70 (15%)

Query: 76  TKSQNAAGSSSADDNTPPLVPEPSVPNNEPAVNTGDWRTQQPQDSRQKNINALLDTLKKI 135
           T+  + A S   D N P            PA+  GDWR+Q   ++R + +N ++DTLKK 
Sbjct: 12  TQGADPAASGGIDPNAPA-----------PALAGGDWRSQLQSEARNRIVNKIMDTLKKH 60

Query: 136 VPHSGKEGID 145
           +P S  EG++
Sbjct: 61  LPVSVPEGLN 70
>gi|24461867|gb|AAN62354.1|AF506028_23   CTV.22 [Poncirus trifoliata]
          Length = 1405

 Score = 92.8 bits (229), Expect = 8e-19
 Identities = 51/87 (58%), Positives = 66/87 (75%)

Query: 4   TGDWRTQFPSASRSRIVNKIMETQLKQLPFIRPEGTNELRKIAVRFEEKLFNNASNQTEY 63
           TGDWRTQ    SR RIVNKIM+T  + LPF   +G NEL+KIA RFEEK++  AS+Q++Y
Sbjct: 34  TGDWRTQLQPDSRQRIVNKIMDTLKRHLPFSGQDGLNELKKIAGRFEEKIYTAASSQSDY 93

Query: 64  LRQICMKMLNMETKSQNAAGSSSADDN 90
           LR+I +KML+ME+KSQNA  +S   +N
Sbjct: 94  LRKISLKMLSMESKSQNAMPNSLQSNN 120

 Score = 49.7 bits (117), Expect = 7e-06
 Identities = 23/48 (47%), Positives = 36/48 (75%)

Query: 98  PSVPNNEPAVNTGDWRTQQPQDSRQKNINALLDTLKKIVPHSGKEGID 145
           P+ P  E  ++TGDWRTQ   DSRQ+ +N ++DTLK+ +P SG++G++
Sbjct: 23  PTPPVGESNLDTGDWRTQLQPDSRQRIVNKIMDTLKRHLPFSGQDGLN 70
>gi|27260984|dbj|BAC45101.1|   OJ1705_C03.23 [Oryza sativa (japonica cultivar-group)]
          Length = 414

 Score = 37.0 bits (84), Expect = 0.049
 Identities = 19/64 (29%), Positives = 34/64 (53%)

Query: 6  DWRTQFPSASRSRIVNKIMETQLKQLPFIRPEGTNELRKIAVRFEEKLFNNASNQTEYLR 65
          DWRT+     R R+   I+ +   +L         +L+K+A R EE+++  A +  +YLR
Sbjct: 12 DWRTRLGQDIRDRVKRDILFSLQMKLQTTTSTTLIDLQKVAARIEERIYKIAIDFGDYLR 71

Query: 66 QICM 69
          +I +
Sbjct: 72 RISL 75
>gi|7493813|pir||T18235   transcription activator GAL11 homolog - yeast (Candida albicans)
 gi|3859719|emb|CAA21993.1|   possible regulatory protein [Candida albicans]
          Length = 1145

 Score = 33.5 bits (75), Expect = 0.60
 Identities = 23/85 (27%), Positives = 41/85 (48%), Gaps = 2/85 (2%)

Query: 7   WRTQFPSASRSRIVNKIMETQLKQLPFIRPE-GTNELRKIAVRFEEKLFNNASNQTEYLR 65
           WR  +    R ++V  I+ T L +L    P      L K+A  FE+ ++  ++++ +YLR
Sbjct: 23  WRAMYSGEERQKVVQIIINT-LTELHGSNPNFNVQRLSKMAQDFEKLVYERSASKEDYLR 81

Query: 66  QICMKMLNMETKSQNAAGSSSADDN 90
            I MK+  +  + Q  A +     N
Sbjct: 82  AIKMKVHQLRVQKQQIAANQGGQIN 106
  Database: All non-redundant GenBank CDS
  translations+PDB+SwissProt+PIR+PRF
    Posted date:  Jun 26, 2003  1:47 AM
  Number of letters in database: 468,778,868
  Number of sequences in database:  1,459,938
  
Lambda     K      H
   0.311    0.128    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 135,718,278
Number of Sequences: 1459938
Number of extensions: 5456488
Number of successful extensions: 15717
Number of sequences better than  1.0: 14
Number of HSP's better than  1.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 15697
Number of HSP's gapped (non-prelim): 26
length of query: 145
length of database: 468,778,868
effective HSP length: 121
effective length of query: 24
effective length of database: 292,126,370
effective search space: 7011032880
effective search space used: 7011032880
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)