RID=1057142214-021300-11363, Ca_Gal11 BLASTP 2.2.6 [Apr-09-2003]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

RID: 1057142214-021300-11363

Query= Ca_Gal11 (138 letters)

Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 1,463,011 sequences; 469,658,079 total letters

If you have any problems or questions with the results of this search
please refer to the BLAST FAQs




Results of PSI-Blast iteration 2
Skip to the first new sequence


Distribution of 14 Blast Hits on the Query Sequence



Legend:

New sequence mark - means that the alignment score was below the threshold on the previous iteration

Checked mark - means that the alignment was checked on the previous iteration


Hit list size
Sequences with E-value BETTER than threshold
Score E Sequences producing significant alignments: (bits) Value

 Checked mark gi|7493813|pir||T18235 transcription activator GAL11 homolog - y... 181 1e-45
 Checked mark gi|24461860|gb|AAN62347.1|AF506028_14 CTV.20 [Poncirus trifoliata] 141 2e-33
 Checked mark gi|28071313|dbj|BAC56002.1| P0705A05.20 [Oryza sativa (japonica ... 124 3e-28
New sequence mark gi|24461867|gb|AAN62354.1|AF506028_23 CTV.22 [Poncirus trifoliata] 106 8e-23
New sequence mark gi|15218294|ref|NP_173030.1| expressed protein [Arabidopsis thal... 97 4e-20
New sequence mark gi|22329593|ref|NP_173031.2| expressed protein [Arabidopsis thal... 94 3e-19
New sequence mark gi|25511635|pir||B86292 F7H2.12 protein - Arabidopsis thaliana >... 82 2e-15
New sequence mark gi|27260984|dbj|BAC45101.1| OJ1705_C03.23 [Oryza sativa (japonic... 54 6e-07

Sequences with E-value WORSE than threshold

  gi|19074594|ref|NP_586100.1| MADS domain [Encephalitozoon cunicu... 40 0.008
  gi|15226772|ref|NP_178842.1| hypothetical protein [Arabidopsis t... 36 0.098
  gi|7511830|pir||T13828 CREB-binding protein homolog - fruit fly ... 35 0.28 LocusLink info
  gi|24640865|ref|NP_524642.2| nejire CG15319-PB [Drosophila melan... 34 0.34 LocusLink info
  gi|1169834|sp|P32257|GALY_KLULA TRANSCRIPTION REGULATORY PROTEIN... 33 0.71

Alignments
>gi|7493813|pir||T18235   transcription activator GAL11 homolog - yeast (Candida albicans)
 gi|3859719|emb|CAA21993.1|   possible regulatory protein [Candida albicans]
          Length = 1145

 Score =  181 bits (460), Expect = 1e-45
 Identities = 122/122 (100%), Positives = 122/122 (100%)

Query: 1   GGGSNPNASWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERS 60
           GGGSNPNASWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERS
Sbjct: 14  GGGSNPNASWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERS 73

Query: 61  ASKEDYLRAIKMKVHQLRVQKQQIAANQGGQINPQQRQQQQQQQISNSNSMNPVNAQNVQ 120
           ASKEDYLRAIKMKVHQLRVQKQQIAANQGGQINPQQRQQQQQQQISNSNSMNPVNAQNVQ
Sbjct: 74  ASKEDYLRAIKMKVHQLRVQKQQIAANQGGQINPQQRQQQQQQQISNSNSMNPVNAQNVQ 133

Query: 121 FL 122
           FL
Sbjct: 134 FL 135
>gi|24461860|gb|AAN62347.1|AF506028_14   CTV.20 [Poncirus trifoliata]
          Length = 3148

 Score =  141 bits (356), Expect = 2e-33
 Identities = 22/83 (26%), Positives = 44/83 (53%), Gaps = 1/83 (1%)

Query: 5  NPNASWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERSASKE 64
          +P + WR   S + RQ++V  I++TL      +    +  L ++A  FE+ ++  + S+ 
Sbjct: 13 DP-SDWRNPLSHDSRQRIVNKIMDTLKRHLPFSGPEGLNELKRIADRFEEKIFTSATSQS 71

Query: 65 DYLRAIKMKVHQLRVQKQQIAAN 87
          DYLR I +K+  +  + Q  + +
Sbjct: 72 DYLRKISLKMLSMESRSQNASGS 94
>gi|28071313|dbj|BAC56002.1|   P0705A05.20 [Oryza sativa (japonica cultivar-group)]
          Length = 1359

 Score =  124 bits (310), Expect = 3e-28
 Identities = 29/94 (30%), Positives = 47/94 (50%), Gaps = 8/94 (8%)

Query: 2   GGSNPNA--------SWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFE 53
           GG +PNA         WR+    E R ++V  I++TL +    +    +  L K+A  FE
Sbjct: 21  GGIDPNAPAPALAGGDWRSQLQSEARNRIVNKIMDTLKKHLPVSVPEGLNELQKIAVRFE 80

Query: 54  KLVYERSASKEDYLRAIKMKVHQLRVQKQQIAAN 87
           + +Y  + S+ DYLR I +K+  +  + QQ   N
Sbjct: 81  EKIYTAATSQSDYLRKISLKMLSMETKTQQNPGN 114
>gi|24461867|gb|AAN62354.1|AF506028_23   CTV.22 [Poncirus trifoliata]
          Length = 1405

 Score =  106 bits (264), Expect = 8e-23
 Identities = 33/123 (26%), Positives = 55/123 (44%), Gaps = 6/123 (4%)

Query: 9   SWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERSASKEDYLR 68
            WR     + RQ++V  I++TL      +    +  L K+A  FE+ +Y  ++S+ DYLR
Sbjct: 36  DWRTQLQPDSRQRIVNKIMDTLKRHLPFSGQDGLNELKKIAGRFEEKIYTAASSQSDYLR 95

Query: 69  AIKMKVHQLRVQKQQIAANQGGQINPQQRQQ-----QQQQQISNSNSMNPVN-AQNVQFL 122
            I +K+  +  + Q    N     NP    +       Q Q+ N     P+  + N   +
Sbjct: 96  KISLKMLSMESKSQNAMPNSLQSNNPGSSNRPPDPGSMQNQVHNQGQSLPIPLSANQSQV 155

Query: 123 RQQ 125
           RQQ
Sbjct: 156 RQQ 158
>gi|15218294|ref|NP_173030.1|   expressed protein [Arabidopsis thaliana]
          Length = 1335

 Score = 97.1 bits (240), Expect = 4e-20
 Identities = 22/79 (27%), Positives = 40/79 (50%)

Query: 9  SWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERSASKEDYLR 68
           WR     + RQK+V  I+ TL +    +    +  L ++A  FE+ ++  + ++ DYLR
Sbjct: 21 DWRTQLPPDSRQKIVNKIMETLKKHLPFSGPEGINELRRIAARFEEKIFSGALNQTDYLR 80

Query: 69 AIKMKVHQLRVQKQQIAAN 87
           I MK+  +  + Q  A +
Sbjct: 81 KISMKMLTMETKSQNAAGS 99
>gi|22329593|ref|NP_173031.2|   expressed protein [Arabidopsis thaliana]
 gi|25513497|pir||C86292   F7H2.13 protein - Arabidopsis thaliana
 gi|8927658|gb|AAF82149.1|AC034256_13   ESTs gb|AI995735, gb|T44391, gb|AA395434 come from this gene.
          [Arabidopsis thaliana]
 gi|12083256|gb|AAG48787.1|AF332424_1   unknown protein [Arabidopsis thaliana]
          Length = 179

 Score = 94.4 bits (233), Expect = 3e-19
 Identities = 23/90 (25%), Positives = 42/90 (46%), Gaps = 2/90 (2%)

Query: 5  NPNASWRAMYSGEERQKVVQIIINT-LTELHGSNPNFNVQRLSKMAQDFEKLVYERSASK 63
          +    WR  +    R ++V  I+ T L +L    P      L K+A  FE+ ++  ++++
Sbjct: 2  DTTGDWRTQFPSASRSRIVNKIMETQLKQLPFIRPE-GTNELRKIAVRFEEKLFNNASNQ 60

Query: 64 EDYLRAIKMKVHQLRVQKQQIAANQGGQIN 93
           +YLR I MK+  +  + Q  A +     N
Sbjct: 61 TEYLRQICMKMLNMETKSQNAAGSSSADDN 90

 Score = 73.2 bits (178), Expect = 7e-13
 Identities = 17/69 (24%), Positives = 37/69 (53%)

Query: 9   SWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERSASKEDYLR 68
            WR     + RQK +  +++TL ++   +    +  L ++A   E+L++  + ++EDYL 
Sbjct: 111 DWRTQQPQDSRQKNINALLDTLKKIVPHSGKEGIDELMRIAVSLEELIFNSAINQEDYLG 170

Query: 69  AIKMKVHQL 77
            I +K+  +
Sbjct: 171 KISLKMRTM 179
>gi|25511635|pir||B86292   F7H2.12 protein - Arabidopsis thaliana
 gi|8927657|gb|AAF82148.1|AC034256_12   EST gb|N38213 comes from this gene. [Arabidopsis thaliana]
          Length = 1366

 Score = 81.7 bits (200), Expect = 2e-15
 Identities = 22/110 (20%), Positives = 40/110 (36%), Gaps = 31/110 (28%)

Query: 9   SWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERSASK----- 63
            WR     + RQK+V  I+ TL +    +    +  L ++A  FE+ ++  + ++     
Sbjct: 21  DWRTQLPPDSRQKIVNKIMETLKKHLPFSGPEGINELRRIAARFEEKIFSGALNQRFVRQ 80

Query: 64  --------------------------EDYLRAIKMKVHQLRVQKQQIAAN 87
                                      DYLR I MK+  +  + Q  A +
Sbjct: 81  WTPQHGKELTFGICKAKPQYVGYEIHTDYLRKISMKMLTMETKSQNAAGS 130
>gi|27260984|dbj|BAC45101.1|   OJ1705_C03.23 [Oryza sativa (japonica cultivar-group)]
          Length = 414

 Score = 53.5 bits (127), Expect = 6e-07
 Identities = 17/71 (23%), Positives = 31/71 (43%)

Query: 2  GGSNPNASWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERSA 61
          G       WR     + R +V + I+ +L     +  +  +  L K+A   E+ +Y+ + 
Sbjct: 5  GIIEEGVDWRTRLGQDIRDRVKRDILFSLQMKLQTTTSTTLIDLQKVAARIEERIYKIAI 64

Query: 62 SKEDYLRAIKM 72
             DYLR I +
Sbjct: 65 DFGDYLRRISL 75
>gi|19074594|ref|NP_586100.1|   MADS domain [Encephalitozoon cuniculi]
 gi|19069236|emb|CAD25704.1|   MADS domain [Encephalitozoon cuniculi]
          Length = 776

 Score = 39.7 bits (91), Expect = 0.008
 Identities = 25/104 (24%), Positives = 45/104 (43%), Gaps = 14/104 (13%)

Query: 13  MYSGEERQKVVQIIINTLTE---LHGSNPNFNVQRLSKMAQDFEKLVYERSASKEDYLRA 69
           M S EER++++  +   L E     G  P     ++ + A   E+ +YE S SKE+YLR 
Sbjct: 1   MLSQEERKQMILKLFVALKESNIFPGYTPV----QIKEAAIRAEQRIYEESGSKEEYLRG 56

Query: 70  IKMKVHQLRVQKQQIAANQGGQINPQQRQQQQQQQISNSNSMNP 113
           +  +  ++       +   G       R +  Q+Q+ +     P
Sbjct: 57  MDERFQRIERVVSNRSRADG-------RGKSTQEQMGSYEHARP 93
>gi|15226772|ref|NP_178842.1|   hypothetical protein [Arabidopsis thaliana]
 gi|25411357|pir||E84491   hypothetical protein At2g10440 [imported] - Arabidopsis thaliana
 gi|4733973|gb|AAD28655.1|   hypothetical protein [Arabidopsis thaliana]
          Length = 935

 Score = 36.2 bits (82), Expect = 0.098
 Identities = 14/55 (25%), Positives = 25/55 (45%), Gaps = 3/55 (5%)

Query: 9  SWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERSASK 63
           WR+ +  E RQKV+  I+    E   ++  + +     +A  FE+  Y  +  K
Sbjct: 24 DWRSQHEPELRQKVLSKIVEKFKEKFHAHEEYKIND---IASKFEENFYSIATDK 75
>gi|7511830|pir||T13828   CREB-binding protein homolog - fruit fly (Drosophila melanogaster)
 gi|1916930|gb|AAB53050.1|  LocusLink info CREB-binding protein homolog [Drosophila melanogaster]
          Length = 3190

 Score = 34.7 bits (78), Expect = 0.28
 Identities = 14/76 (18%), Positives = 35/76 (46%), Gaps = 2/76 (2%)

Query: 5    NPNASWRAMYSGEERQKVVQIIINTL--TELHGSNPNFNVQRLSKMAQDFEKLVYERSAS 62
            +    WR   + + R  +V  ++  +  T    +  +  +  L   A+  EK +YE + S
Sbjct: 938  DNEKDWRESVTADLRNHLVHKLVQAIFPTSDPTTMQDKRMHNLVSYAEKVEKDMYEMAKS 997

Query: 63   KEDYLRAIKMKVHQLR 78
            + +Y   +  K+++++
Sbjct: 998  RSEYYHLLAEKIYKIQ 1013
>gi|24640865|ref|NP_524642.2|  LocusLink info nejire CG15319-PB [Drosophila melanogaster]
 gi|22833041|gb|AAF46516.2|  LocusLink info CG15319-PB [Drosophila melanogaster]
          Length = 3276

 Score = 34.3 bits (77), Expect = 0.34
 Identities = 14/76 (18%), Positives = 35/76 (46%), Gaps = 2/76 (2%)

Query: 5    NPNASWRAMYSGEERQKVVQIIINTL--TELHGSNPNFNVQRLSKMAQDFEKLVYERSAS 62
            +    WR   + + R  +V  ++  +  T    +  +  +  L   A+  EK +YE + S
Sbjct: 938  DNEKDWRESVTADLRNHLVHKLVQAIFPTSDPTTMQDKRMHNLVSYAEKVEKDMYEMAKS 997

Query: 63   KEDYLRAIKMKVHQLR 78
            + +Y   +  K+++++
Sbjct: 998  RSEYYHLLAEKIYKIQ 1013
>gi|1169834|sp|P32257|GALY_KLULA   TRANSCRIPTION REGULATORY PROTEIN GAL11
 gi|101140|pir||S19033   transcription activator GAL11 - yeast (Kluyveromyces marxianus
          var. lactis) (fragment)
 gi|295684|gb|AAA35254.1|   GAL11 transcription activator
          Length = 1008

 Score = 33.1 bits (74), Expect = 0.71
 Identities = 15/45 (33%), Positives = 28/45 (62%)

Query: 39 NFNVQRLSKMAQDFEKLVYERSASKEDYLRAIKMKVHQLRVQKQQ 83
          NFN+++L K A+ FE  +Y    SK+ YL A++ ++  +   K++
Sbjct: 11 NFNLEKLKKSAEQFESSLYASCNSKDLYLDAMRKRIAAMDSAKRK 55
  Database: All non-redundant GenBank CDS
  translations+PDB+SwissProt+PIR+PRF
    Posted date:  Jul 1, 2003  3:46 AM
  Number of letters in database: 469,658,079
  Number of sequences in database:  1,463,011
  
Lambda     K      H
   0.317    0.143    0.380 

Gapped
Lambda     K      H
   0.267   0.0436    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 108,415,154
Number of Sequences: 1463011
Number of extensions: 3887631
Number of successful extensions: 87123
Number of sequences better than  1.0: 100
Number of HSP's better than  1.0 without gapping: 459
Number of HSP's successfully gapped in prelim test: 329
Number of HSP's that attempted gapping in prelim test: 55391
Number of HSP's gapped (non-prelim): 10862
length of query: 138
length of database: 469,658,079
effective HSP length: 114
effective length of query: 24
effective length of database: 302,874,825
effective search space: 7268995800
effective search space used: 7268995800
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)