RID=1057142147-020616-14243, Ca_Gal11 BLASTP 2.2.6 [Apr-09-2003]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

RID: 1057142147-020616-14243

Query= Ca_Gal11 (138 letters)

Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 1,463,011 sequences; 469,658,079 total letters

If you have any problems or questions with the results of this search
please refer to the BLAST FAQs




Results of PSI-Blast iteration 1

Distribution of 9 Blast Hits on the Query Sequence



Legend:

New sequence mark - means that the alignment score was below the threshold on the previous iteration

Checked mark - means that the alignment was checked on the previous iteration


Hit list size
Sequences with E-value BETTER than threshold
Score E Sequences producing significant alignments: (bits) Value

New sequence mark gi|7493813|pir||T18235 transcription activator GAL11 homolog - y... 177 3e-44
New sequence mark gi|28071313|dbj|BAC56002.1| P0705A05.20 [Oryza sativa (japonica ... 44 5e-04
New sequence mark gi|24461860|gb|AAN62347.1|AF506028_14 CTV.20 [Poncirus trifoliata] 42 0.001

Sequences with E-value WORSE than threshold

  gi|24461867|gb|AAN62354.1|AF506028_23 CTV.22 [Poncirus trifoliata] 39 0.012
  gi|15218294|ref|NP_173030.1| expressed protein [Arabidopsis thal... 38 0.025
  gi|22329593|ref|NP_173031.2| expressed protein [Arabidopsis thal... 36 0.082
  gi|19074594|ref|NP_586100.1| MADS domain [Encephalitozoon cunicu... 34 0.37
  gi|1169834|sp|P32257|GALY_KLULA TRANSCRIPTION REGULATORY PROTEIN... 33 0.73

Alignments
>gi|7493813|pir||T18235   transcription activator GAL11 homolog - yeast (Candida albicans)
 gi|3859719|emb|CAA21993.1|   possible regulatory protein [Candida albicans]
          Length = 1145

 Score =  177 bits (449), Expect = 3e-44
 Identities = 99/122 (81%), Positives = 99/122 (81%)

Query: 1   GGGSNPNASWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERS 60
           GGGSNPNASWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERS
Sbjct: 14  GGGSNPNASWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERS 73

Query: 61  ASKEDYLRAIKMKVHQLRVQKQQIAAXXXXXXXXXXXXXXXXXXXXXXXSMNPVNAQNVQ 120
           ASKEDYLRAIKMKVHQLRVQKQQIAA                       SMNPVNAQNVQ
Sbjct: 74  ASKEDYLRAIKMKVHQLRVQKQQIAANQGGQINPQQRQQQQQQQISNSNSMNPVNAQNVQ 133

Query: 121 FL 122
           FL
Sbjct: 134 FL 135
>gi|28071313|dbj|BAC56002.1|   P0705A05.20 [Oryza sativa (japonica cultivar-group)]
          Length = 1359

 Score = 43.5 bits (101), Expect = 5e-04
 Identities = 28/90 (31%), Positives = 46/90 (51%), Gaps = 8/90 (8%)

Query: 2   GGSNPNA--------SWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFE 53
           GG +PNA         WR+    E R ++V  I++TL +    +    +  L K+A  FE
Sbjct: 21  GGIDPNAPAPALAGGDWRSQLQSEARNRIVNKIMDTLKKHLPVSVPEGLNELQKIAVRFE 80

Query: 54  KLVYERSASKEDYLRAIKMKVHQLRVQKQQ 83
           + +Y  + S+ DYLR I +K+  +  + QQ
Sbjct: 81  EKIYTAATSQSDYLRKISLKMLSMETKTQQ 110
>gi|24461860|gb|AAN62347.1|AF506028_14   CTV.20 [Poncirus trifoliata]
          Length = 3148

 Score = 42.4 bits (98), Expect = 0.001
 Identities = 24/76 (31%), Positives = 42/76 (55%), Gaps = 2/76 (2%)

Query: 8  ASWRAMYSGEERQKVVQIIINTLT-ELHGSNPNFNVQRLSKMAQDFEKLVYERSASKEDY 66
          + WR   S + RQ++V  I++TL   L  S P   +  L ++A  FE+ ++  + S+ DY
Sbjct: 15 SDWRNPLSHDSRQRIVNKIMDTLKRHLPFSGPE-GLNELKRIADRFEEKIFTSATSQSDY 73

Query: 67 LRAIKMKVHQLRVQKQ 82
          LR I +K+  +  + Q
Sbjct: 74 LRKISLKMLSMESRSQ 89
>gi|24461867|gb|AAN62354.1|AF506028_23   CTV.22 [Poncirus trifoliata]
          Length = 1405

 Score = 39.3 bits (90), Expect = 0.012
 Identities = 25/82 (30%), Positives = 42/82 (51%), Gaps = 1/82 (1%)

Query: 2   GGSN-PNASWRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERS 60
           G SN     WR     + RQ++V  I++TL      +    +  L K+A  FE+ +Y  +
Sbjct: 28  GESNLDTGDWRTQLQPDSRQRIVNKIMDTLKRHLPFSGQDGLNELKKIAGRFEEKIYTAA 87

Query: 61  ASKEDYLRAIKMKVHQLRVQKQ 82
           +S+ DYLR I +K+  +  + Q
Sbjct: 88  SSQSDYLRKISLKMLSMESKSQ 109
>gi|15218294|ref|NP_173030.1|   expressed protein [Arabidopsis thaliana]
          Length = 1335

 Score = 38.1 bits (87), Expect = 0.025
 Identities = 22/76 (28%), Positives = 39/76 (51%)

Query: 10 WRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERSASKEDYLRA 69
          WR     + RQK+V  I+ TL +    +    +  L ++A  FE+ ++  + ++ DYLR 
Sbjct: 22 WRTQLPPDSRQKIVNKIMETLKKHLPFSGPEGINELRRIAARFEEKIFSGALNQTDYLRK 81

Query: 70 IKMKVHQLRVQKQQIA 85
          I MK+  +  + Q  A
Sbjct: 82 ISMKMLTMETKSQNAA 97
>gi|22329593|ref|NP_173031.2|   expressed protein [Arabidopsis thaliana]
 gi|25513497|pir||C86292   F7H2.13 protein - Arabidopsis thaliana
 gi|8927658|gb|AAF82149.1|AC034256_13   ESTs gb|AI995735, gb|T44391, gb|AA395434 come from this gene.
           [Arabidopsis thaliana]
 gi|12083256|gb|AAG48787.1|AF332424_1   unknown protein [Arabidopsis thaliana]
          Length = 179

 Score = 36.2 bits (82), Expect = 0.082
 Identities = 17/68 (25%), Positives = 37/68 (54%)

Query: 10  WRAMYSGEERQKVVQIIINTLTELHGSNPNFNVQRLSKMAQDFEKLVYERSASKEDYLRA 69
           WR     + RQK +  +++TL ++   +    +  L ++A   E+L++  + ++EDYL  
Sbjct: 112 WRTQQPQDSRQKNINALLDTLKKIVPHSGKEGIDELMRIAVSLEELIFNSAINQEDYLGK 171

Query: 70  IKMKVHQL 77
           I +K+  +
Sbjct: 172 ISLKMRTM 179

 Score = 34.7 bits (78), Expect = 0.28
 Identities = 22/77 (28%), Positives = 39/77 (50%), Gaps = 2/77 (2%)

Query: 10 WRAMYSGEERQKVVQIIINT-LTELHGSNPNFNVQRLSKMAQDFEKLVYERSASKEDYLR 68
          WR  +    R ++V  I+ T L +L    P      L K+A  FE+ ++  ++++ +YLR
Sbjct: 7  WRTQFPSASRSRIVNKIMETQLKQLPFIRPE-GTNELRKIAVRFEEKLFNNASNQTEYLR 65

Query: 69 AIKMKVHQLRVQKQQIA 85
           I MK+  +  + Q  A
Sbjct: 66 QICMKMLNMETKSQNAA 82
>gi|19074594|ref|NP_586100.1|   MADS domain [Encephalitozoon cuniculi]
 gi|19069236|emb|CAD25704.1|   MADS domain [Encephalitozoon cuniculi]
          Length = 776

 Score = 34.3 bits (77), Expect = 0.37
 Identities = 22/67 (32%), Positives = 36/67 (53%), Gaps = 5/67 (7%)

Query: 13 MYSGEERQKVVQIIINTLTELHGSN--PNFNVQRLSKMAQDFEKLVYERSASKEDYLRAI 70
          M S EER+   Q+I+     L  SN  P +   ++ + A   E+ +YE S SKE+YLR +
Sbjct: 1  MLSQEERK---QMILKLFVALKESNIFPGYTPVQIKEAAIRAEQRIYEESGSKEEYLRGM 57

Query: 71 KMKVHQL 77
            +  ++
Sbjct: 58 DERFQRI 64
>gi|1169834|sp|P32257|GALY_KLULA   TRANSCRIPTION REGULATORY PROTEIN GAL11
 gi|101140|pir||S19033   transcription activator GAL11 - yeast (Kluyveromyces marxianus
          var. lactis) (fragment)
 gi|295684|gb|AAA35254.1|   GAL11 transcription activator
          Length = 1008

 Score = 33.1 bits (74), Expect = 0.73
 Identities = 16/49 (32%), Positives = 29/49 (59%)

Query: 35 GSNPNFNVQRLSKMAQDFEKLVYERSASKEDYLRAIKMKVHQLRVQKQQ 83
          G   NFN+++L K A+ FE  +Y    SK+ YL A++ ++  +   K++
Sbjct: 7  GEKANFNLEKLKKSAEQFESSLYASCNSKDLYLDAMRKRIAAMDSAKRK 55
  Database: All non-redundant GenBank CDS
  translations+PDB+SwissProt+PIR+PRF
    Posted date:  Jul 1, 2003  3:46 AM
  Number of letters in database: 469,658,079
  Number of sequences in database:  1,463,011
  
Lambda     K      H
   0.314    0.128    0.351 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,146,130
Number of Sequences: 1463011
Number of extensions: 2252021
Number of successful extensions: 7831
Number of sequences better than  1.0: 8
Number of HSP's better than  1.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 7822
Number of HSP's gapped (non-prelim): 9
length of query: 138
length of database: 469,658,079
effective HSP length: 114
effective length of query: 24
effective length of database: 302,874,825
effective search space: 7268995800
effective search space used: 7268995800
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)